47 results about "Soybean cyst nematode" patented technology

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased soybean cyst nematode (SCN) resistance are provided. Also provided are methods of breeding plants having alleles associated with increased SCN resistance and methods of identifying and selecting plants having alleles associated with increased SCN resistance. Transgenes and genome-edited plants comprising gene alleles associated with increased SCN resistance are provided.

The present application relates to "application of a compound in soybean cyst nematode chitinase inhibitor", belong to the technical field of leguminous plant symbiotic protection, the application of the compound and its pharmaceutically acceptable salt in soybean cyst nematode chitinase inhibitor, the present application is evaluated and researched through the inhibitory activity of compound by screening work, the inhibitor is screened finally, the inhibitor is likely to have good application prospect in controlling soybean cyst nematode disease aspect.The present application also provides a kind of chitinase inhibitor with the function of inhibiting the establishment of symbiosis of soybean cyst nematode to destroy, the number of nodule obtained by processing is flat with the number when only inoculating rhizobium, the inhibitory effect of Hg-CHI-1 on rhizobium nodule is completely restored, and it has good application prospect in the aspect of leguminous plant symbiotic protection.

The invention provides a primer combination for identifying a single-copy gene of Australian cyst nematode. The primer combination comprises an upstream outer primer HAUS-F3, a downstream outer primer HAUS-B3, an upstream inner primer HAUS-FIP, a downstream inner primer HAUS-BIP and a downstream loop primer HAUS-LB. The primer combination can specifically distinguish the Australian cyst nematode from other common cyst nematodes such as cereal cyst nematode, Philipse cyst nematode, corn cyst nematode and soybean cyst nematode, the detection limit is low, the detection time is short, and the detection result is identified by means of dye color development or electrophoretogram. The technology is easy to operate, good in sensitivity, high in specificity and high in reaction speed, the result can be directly observed without additional instruments, and the method has important significance in the aspects of rapid detection and early field diagnosis of the Australian cyst nematode.

The invention provides bacillus megatherium for preventing and treating soil-borne diseases of crops, a microbial inoculum and application of the bacillus megatherium and the microbial inoculum. The bacillus megaterium HN-2 disclosed by the invention is classified and named as bacillus megaterium, the bacillus megaterium HN-2 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the bacillus megaterium HN-2 is CGMCC No.34657. A test result shows that the antibacterial effect of the bacillus megaterium HN-2 on four kinds of fusarium and tobacco black shank bacteria reaches 60.98%-71.12%; the indoor nematode killing effect on wheat cyst nematode and tomato root-knot nematode reaches 100%. The optimized binder, wetting agent, stabilizing agent and disintegrating agent are respectively starch, lauryl sodium sulfate, CaCO3 and AlCl3; after seed dressing and broadcasting, the field cyst decline rates of wheat cyst nematode and soybean cyst nematode are 60.22% and 52.58% respectively, the field prevention effects of wheat root rot and soybean root rot are 69.50% and 54.44% respectively, and the biocontrol inoculant has wide application potential.

The invention discloses a soybean cyst nematode No.5 physiological race resistance associated KASP molecular marker, a primer and application, and relates to the technical field of gene engineering. The soybean cyst nematode 5 physiological race resistance associated KASP molecular markers are SNP molecular markers SCN2.1, SCN2.2 and SCN2.3, the SCN2.1 has a pair of primers including a forward primer and a reverse primer, the SCN2.2 has a pair of primers including a forward primer and a reverse primer, the SCN2.3 has a pair of primers including a forward primer and a reverse primer, and the kit contains the primers of the KASP molecular markers. The KASP molecular marker is applied to the breeding of the soybean cyst nematode No.5 physiological race. The invention provides a set of complete technical solution which comprises a KASP molecular marker directly associated with resistance, a matched specific detection primer combination and a rapid detection and application method, early-stage, high-throughput and accurate identification of soybean anti-soybean cyst nematode 5 physiological race traits is realized, and the disease-resistant breeding efficiency is remarkably improved.

The invention provides bacillus methylotrophicus N7, a preparation and application of the bacillus methylotrophicus N7 to prevention and control of soybean cyst nematode, and belongs to the technical field of microorganisms. The preservation number of the bacillus methylotrophicus N7 is CGMCC (China General Microbiological Culture Collection Center) No.31391, and the bacillus methylotrophicus N7 is obtained by mutagenizing an original strain The strain is suitable for soybean low-temperature producing areas at 5-20 DEG C, has high soybean cyst nematode resistance, the cyst inhibition rate of a high-concentration group in the seedling stage is over 70%, the soybean yield in the mature stage is increased by 24.6% compared with a blank control, and the strain is non-toxic, free of residues and eco-friendly to soil.

Provided are plants, cells, tissues, and germplasm thereof comprising gene and marker alleles associated with increased resistance to soybean cyst nematode (SCN). Also provided are breeding methods and methods of identifying and selecting plants having alleles associated with increased resistance to SCN. Transgenic and genome edited plants comprising the gene alleles associated with increased SCN resistance are provided.

The invention provides an SNP molecular marker related to soybean cyst nematode disease resistance, a detection primer and application of the SNP molecular marker and the detection primer, and belongs to the technical field of plant breeding and molecular biology. The molecular marker is located at the 797th site of the ninth exon region of the soybean SNAP18 gene, and Ggt exists; a single nucleotide polymorphism (SNP). A specific KASP primer is designed on the basis of the site, the resistance of soybeans to cyst nematode can be accurately identified through genotype detection, and excellent phenotypes with scab-free characters can be screened at the same time. The method is high in detection accuracy, has the advantages of being accurate, rapid and low in cost, can be used for screening soybean cyst nematode resistance and / or scab-free excellent germplasm resources and molecular marker-assisted selective breeding, and remarkably improves the disease-resistant breeding efficiency.

Compositions and methods for conferring nematicidal activity upon bacteria, plants, plant cells, tissues, and seeds are provided. Methods for killing or controlling populations of nematode pests, such as cyst nematode species, for example, *Heterodera glycines* (soybean cyst nematode), root-knot nematodes, kidney-shaped nematodes, short-bodied nematode species, or lanceolate nematode populations, are also provided. These methods further include contacting the nematode pest with a pest-killing amount of a polypeptide containing a nematicidal toxin. Further methods include increasing plant yield by expressing genes of the embodiments in plants.

The invention discloses application of soybean GmVQ70 to improvement of soybean cyst nematode resistance, relates to the technical field of genetic engineering, and solves the problems that existing chemical control pollutes the environment and is difficult to implement crop rotation, and traditional breeding is long in period and prone to loss of resistance. The method comprises the following steps: 1, constructing an overexpression vector of the GmVQ70 gene to obtain a transformed strain; and 2, infecting soybean plant tissues by the transformed strain. The invention proves that the soybean GmVQ70 gene has the effect of regulating and controlling soybean cyst nematode resistance of soybeans for the first time. A receptor soybean plant is subjected to genetic transformation by using an overexpression vector of the GmVQ70 gene, and after soybean cyst nematode infection, the number of soybean root surface female insects can be remarkably reduced by 38.4% compared with a wild type, so that the resistance of soybeans to soybean cyst nematode is improved, and a gene resource is provided for soybean resistance breeding. The application and the method of the GmVQ70 gene play a key role in improving the resistance process of soybean cyst nematode, and a new molecular target can be provided for soybean precise molecular design breeding.

The invention belongs to the technical field of plant molecular breeding, and discloses a molecular marker related to soybean cyst nematode disease resistance and application thereof. The CAPS molecular marker provided by the invention is simple to match and operate, and can quickly distinguish three genotypes of susceptibility, disease resistance and excellent phenotype and disease resistance and scabs. The molecular marker does not need expensive detection equipment, is simple and convenient to operate, low in cost and accurate in typing, is suitable for screening soybean cyst nematode-resistant filial generations, identifying transgenic lines and detecting the purity of disease-resistant varieties, can remarkably improve the breeding efficiency and shorten the breeding period, and provides a practical technical tool for soybean cyst nematode resistance breeding.

Compositions and methods for conferring nematodic activity to bacteria, plants, plant cells, tissues, and seeds are provided. Furthermore, nematode pest populations, particularly Platylenchus species (Pratylenchus spp.), such as pineapple root-knot nematode (Pratylenchus brachyurus), northern root-knot nematode (Pratylenchus penetrans), scribneri, neglectus nematode (Pratylenchus neglectus), Platylenchus zeae, long-tailed lance nematode (Belonolaimus longicaudatus), Hoplolainus galeatus, Helicotylenchus dihystera, spiral nematode (Helicotylenchus pseudorobustus), or Xiphinema nematode (Xiphinema Methods are provided for killing or controlling populations of Americanum nematodes, root-knot nematodes, false sedge nematodes, soybean cyst nematodes, or lance nematodes. The methods include contacting nematode pests with a pesticidal effective amount of a polypeptide containing a nematode toxin. Furthermore, methods for increasing plant yield by expressing the genes of the present invention in plants are also provided.

Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Further provided are methods for killing or controlling a population of nematode pests, in particular Pratylenchus species, the present invention relates to a population of nematodes, such as Pratylenchus brachycaeus, Pratylenchus punctatus, Pratylenchus schriberi, Pratylenchus neglectus, Pratylenchus zeae, Blonolaimus longicaudatus, Hoplolaimus galeae, Helicida dihysteria, Helicida pseudomonas, or Xiphinea americana, a root-knot nematode, a reniform nematode, a soybean cyst nematode, or a spear nematode population, for example, a population of nematodes, such as Pratylenchus brachycaeus, Pratylenchus sp., Pratylenchus sp. The methods comprise contacting the nematode pest with a pesticidally effective amount of a polypeptide comprising a nematicidal toxin. Further included are methods of increasing plant yield by expressing a gene of an embodiment in a plant.

Provided are plants, cells, tissues, and germplasm thereof comprising gene and marker alleles associated with increased resistance to soybean cyst nematode (SCN). Also provided are breeding methods and methods of identifying and selecting plants having alleles associated with increased resistance to SCN. Transgenic and genome edited plants comprising the gene alleles associated with increased SCN resistance are provided.

This invention relates to the application of the soybean GmWAK9 gene in improving soybean resistance to soybean cyst nematode disease, belonging to the field of genetic engineering technology. To identify genes in soybeans that possess resistance to soybean cyst nematode disease, and subsequently utilize these genes to enhance soybean's resistance to the disease and cultivate new soybean varieties resistant to soybean cyst nematode disease, this invention cloned the GmWAK9 gene from soybean, constructed a recombinant expression vector containing the GmWAK9 gene, and then obtained soybean roots overexpressing the GmWAK9 gene using Agrobacterium-mediated transformation. Inoculation with soybean cyst nematodes demonstrated that overexpression of the GmWAK9 gene can improve soybean resistance to soybean cyst nematodes. Applying GmWAK9 to soybean breeding through hybridization or genetic transformation can improve the disease resistance of soybean varieties, thereby reducing disease incidence and achieving the goals of increased and stable yields.

The application discloses application of a Glyma.04G199400 gene in regulation of soybean cyst nematode resistance and belongs to the technical field of genetic engineering. The CDS sequence of the Glyma.04G199400 gene is shown as SEQ ID NO. 3. The application mines the gene Glyma.04G199400 which regulates soybean cyst nematode 3 physiological race resistance. It is confirmed that overexpression of the gene can significantly reduce the cyst number on soybean hairy roots and improve the soybean cyst nematode resistance, thereby providing theoretical support for soybean disease resistance mechanism research and providing new gene resources and technical support for soybean trait improvement and high-resistance soybean breeding.

The invention belongs to the technical field of molecular marker-assisted selective breeding, and particularly relates to a method for breeding an anti-cyst nematode No.4 physiological race soybean variety. The invention provides a PCR (Polymerase Chain Reaction) primer combination for screening an anti-cyst nematode No.4 physiological race soybean variety, wherein the PCR primer combination comprises a first PCR primer group, a second PCR primer group and a third PCR primer group; the first PCR primer group consists of primers with nucleotide sequences as shown in SEQ ID NO: 1-3; the second PCR primer group consists of primers with nucleotide sequences as shown in SEQ ID NO: 4-6; and the third PCR primer group consists of primers with nucleotide sequences as shown in SEQ ID NO: 7-9. The invention further provides a method for breeding the cyst nematode-resistant No.4 physiological race soybean variety. When the method is used for marker-assisted selection, the efficiency and accuracy of soybean cyst nematode-resistant breeding can be greatly improved.

The application discloses application of bacillus velezensis LH-BLS107 (CGMCC No. 34785) or an engineering bacterium modified through natural, artificial mutagenesis or gene editing in biological control of plant nematode diseases. Fermentation culture solution, bacterium powder and / or secondary metabolites of the LH-BLS107 or the engineering bacterium thereof are used in wheat, soybean, rice, fruit trees, vegetables and the like. The fermentation culture solution, the bacterium powder and / or the secondary metabolites can effectively prevent and control wheat cyst nematode disease, soybean cyst nematode disease, rice paratylenchus pseudohollandei disease, fruit tree and vegetable root knot nematode disease, and the mortality of the above-mentioned nematodes can reach more than 95%, and when the fermentation culture solution, the bacterium powder and / or the secondary metabolites are compounded with chemical nematocide, the chemical agent dosage can be reduced by 25%-30%.

The invention provides actinomycetes for promoting crop growth and preventing and treating nematode diseases, a microbial inoculum and application of the actinomycetes and the microbial inoculum. According to the present invention, the bivorin-producing streptomyces sp. HN-11 is classified and named as the bivorin-producing streptomyces sp. HN-11, the bivorin-producing streptomyces sp. HN-11 has been preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34658. The bivorin-producing streptomyces sp. HN-11 can be used for preparing the bivorin-producing streptomyces sp. HN-11, and can be used for preparing the bivorin-producing streptomyces sp. HN-11, researches show that the bixenogenic streptomyces HN-11 disclosed by the invention has an antibacterial effect of 53.27%-58.54% on four kinds of fusarium, and can be used for preparing the bixenogenic streptomyces HN-11. The nematode killing effect on wheat cyst nematode and tomato root-knot nematode indoors reaches 100%. The optimized binder, wetting agent, stabilizer and disintegrating agent are respectively polyvinyl alcohol, lauryl sodium sulfate, sodium carboxymethyl cellulose and AlCl3; after seed dressing and broadcasting, the field cyst decline rates of wheat cyst nematode and soybean cyst nematode are 35.56% and 56.70% respectively, the field prevention effects of wheat root rot and soybean root rot are 70.42% and 41.70% respectively, the growth promoting effect is remarkable, and the biocontrol fungicide has wide application potential.

The application relates to the field of biological control, and discloses a root-knot nematode biocontrol microcapsule for inducing soybean immune response. The microcapsule is a concentric spherical core-shell structure, and sequentially comprises an active core layer loaded with a nematicidal metabolite, an intermediate barrier layer composed of PLGA, a biocontrol bacteria carrier layer embedded with biocontrol bacteria spores, and a functional protective layer with pH response and light avoidance functions. Through the above structure, the application realizes rapid directional release of the nematicidal component and long-acting slow release of the biocontrol bacteria, builds a composite defense system composed of exogenous killing and endogenous immunity in the soybean rhizosphere, and improves the biological control efficiency and the plant stress resistance level.

The invention discloses a primer group for detecting a KASP molecular marker and application of the primer group in preparation of a preparation for identifying soybean cyst nematode disease, and belongs to the technical field of molecular assisted breeding. The primer group for detecting the KASP molecular marker disclosed by the invention is as shown in SEQ ID NO. 3 to SEQ ID NO. 5. The KASP molecular marker is located at the 8361148bp position of the No.8 chromosome of the soybean reference genome, and the basic group is C / G. The molecular marker is closely linked with soybean cyst nematode disease, and can be used for preliminary screening of soybean cyst nematode disease resistance, so that molecular marker-assisted breeding is achieved, and the breeding process is accelerated. In production, the molecular marker can be used for detecting and screening soybean cyst nematode resistant plants in the seedling stage, the screened germplasm carrying excellent disease-resistant alleles provides a basis for further phenotype identification and rapid screening and utilization of resistant sources, and the yield per unit area of soybeans is increased.

The invention discloses application of a KASP molecular marker in preparation of a preparation for identifying soybean cyst nematode disease, and belongs to the technical field of molecular assisted breeding. According to the application of the KASP molecular marker in preparing the preparation for identifying the soybean cyst nematode disease, the KASP molecular marker is located at the 32969916bp position of the 11th chromosome of a soybean reference genome, and the basic group is C / A. The molecular marker is closely linked with soybean cyst nematode disease, and can be used for preliminary screening of soybean cyst nematode disease resistance, so that molecular marker-assisted breeding is achieved, and the breeding process is accelerated. In production, the molecular marker can be used for detecting and screening soybean cyst nematode resistant plants in the seedling stage, the screened germplasm carrying excellent disease-resistant alleles provides a basis for further phenotype identification and rapid screening and utilization of resistant sources, and the yield per unit area of soybeans is increased.

The invention provides a soybean cyst nematode identification tracking method and system based on a time sequence image, and relates to the technical field of time sequence identification, and the method comprises the steps: collecting a continuous time sequence microscopic image of a root sample through a microscopic imaging device, carrying out the enhancement standardization of a preprocessing model, executing a multi-scale adaptive segmentation algorithm, and constructing a spatial-temporal feature map, and feature similarity and spatial displacement constraints between adjacent frames are calculated based on a Monte Carlo tree search method, and accurate identification and dynamic tracking of the soybean cyst nematode are realized. The soybean cyst nematode identification accuracy and tracking efficiency are improved, and a scientific basis is provided for crop pest control.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased soybean cyst nematode (SCN) resistance are provided. Also provided are methods of breeding plants having alleles associated with increased SCN resistance, as well as methods of identifying and selecting plants having alleles associated with increased SCN resistance. Transgenic and genome-edited plants comprising gene alleles associated with increased SCN resistance are provided.

The invention discloses application of soybean GmVQ5 in improving soybean cyst nematode resistance, and relates to the technical field of gene engineering. The method solves the problems that in existing soybean cyst nematode prevention and control, a chemical method pollutes the environment, rotation implementation is difficult, a traditional breeding period is long, and resistance is prone to loss. The method comprises the steps that 1, a GmVQ5 gene overexpression vector is constructed to obtain a transformed strain; and 2, infecting soybean plant tissues with the transformed strain to obtain a transgenic soybean plant. The invention proves that the soybean GmVQ5 gene has the effect of regulating and controlling soybean cyst nematode resistance of soybeans for the first time. Infecting soybean root tissues by utilizing the transformed engineering bacteria, and carrying out tissue culture to obtain successfully transformed soybean hair roots; after soybean cyst nematode infection, the number of first-generation female insects at soybean roots is obviously reduced. The application of the GmVQ5 gene and the method thereof play a key role in improving the resistance process of soybean cyst nematode, and a new molecular target can be provided for soybean precise molecular design breeding.

The application provides a methylotrophic bacillus N7, a preparation and application thereof in prevention and control of soybean cyst nematode, and belongs to the technical field of microorganisms. The methylotrophic bacillus N7 has a preservation number of CGMCC No.31391, and is obtained by mutagenizing an original strain N1 with 0.4% ethyl methanesulfonate. The methylotrophic bacillus N7 is suitable for a low-temperature soybean production area with a temperature of 5 to 20 DEG C, has high resistance to soybean cyst nematode, a cyst inhibition rate of a high-concentration group in a seedling stage is higher than 70%, a soybean yield in a mature stage is increased by 24.6% compared with a blank control, is non-toxic and non-residual, and is friendly to soil ecology.

The invention belongs to the technical field of plant genetic engineering, and provides an application of a GmGTT1 or GmTGA15 gene and a protein coded by the GmGTT1 or GmTGA15 gene in resisting soybean paratocyst nematode No.4 race. The nucleotide sequence of the GmGTT1 gene CDS is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the gene is as shown in SEQ ID NO. 2; the nucleotide sequence of the GmTGA15 gene CDS is as shown in SEQ ID NO.3, and the amino acid sequence coded by the gene is as shown in SEQ ID NO.4. The over-expression of the soybean GmTGA15 or GmGTT1 gene can improve the tolerance of the soybean to the SCN4, and the knockout of the gene can reduce the resistance of the soybean to the SCN4. The discovery of the functions of the soybean GmTGA15 or GmGTT1 gene provides a gene resource for soybean cyst nematode disease resistance genetic breeding, and also provides a gene target for soybean SCN resistance molecular breeding.

The application belongs to the technical field of microorganisms, and provides a bacillus cereus with high toxic killing activity to plant parasitic nematodes and application thereof. The strain has been preserved in the China Typical Culture Collection Center of Wuhan University in Hongshan District of Wuhan City in Hubei Province on June 15, 2022, and the preservation number is CCTCC NO: M2022893. The isolated strain of the application has high toxic killing effect on root-knot nematodes such as southern root-knot nematode and cyst nematodes such as soybean cyst nematode, and can be used for biological control of root-knot nematode and cyst nematode diseases.

The invention discloses application of a Glyma. 04G199400 gene in regulation and control of soybean cyst nematode resistance, and belongs to the technical field of gene engineering. The CDS sequence of the Glyma. 04G199400 gene is as shown in SEQ ID NO. 3, and the CDS sequence is as shown in SEQ ID NO. The gene Glyma. 04G199400 for regulating and controlling the resistance of the physiological race 3 of the soybean cyst nematode is excavated, it is proved that overexpression of the gene can remarkably reduce the number of cysts on hairy roots of soybeans, the resistance of the soybean cyst nematode is improved, theoretical support is provided for research of a soybean disease-resistant mechanism, and the gene Glyma. And new gene resources and technical support are provided for soybean character improvement and high-resistance soybean breeding.

The application discloses application of a Glyma.06G319700 gene in regulation of soybean cyst nematode resistance and belongs to the technical field of genetic engineering. The CDS sequence of the Glyma.06G319700 gene is shown as SEQ ID NO. 3. The application mines the gene Glyma.06G319700 related to soybean cyst nematode 3 physiological race resistance. It is confirmed that overexpression of the gene can significantly reduce the cyst number on soybean hairy roots and improve the soybean cyst nematode resistance, thereby providing theoretical support for soybean disease resistance mechanism research and providing new gene resources and technical support for soybean trait improvement and high-resistance soybean breeding.