4 results about "Acid producing bacteria" patented technology

The screening method comprises the following steps: S1, taking a high-temperature yeast sample, adding sterile normal saline, soaking and oscillating, taking supernate, inoculating the supernate into an acetic acid bacteria enrichment culture medium, and culturing at constant temperature to obtain enriched liquid; s2, carrying out gradient dilution on the enrichment liquid, taking a diluent with a proper concentration, coating a calcium plate isolation culture medium with the diluent, and selecting a single colony generating a transparent circle to carry out multi-generation streak purification; s3, inoculating the purified strain to an acidogenic qualitative culture medium, carrying out shake cultivation, centrifuging the fermentation broth, taking the supernatant, neutralizing the supernatant with a NaOH solution until the pH value is 7, adding a FeCl3 solution, and heating in a boiling water bath to generate a rufous flocculent precipitate which is an acidogenic bacterium; and S4, preparing bacterial suspension from the bacterial strains which are qualitatively identified as acid-producing bacteria, inoculating the bacterial suspension to a basic culture medium, and selecting the bacterial strain with the highest acid yield as a target bacterial strain. The strain with high acid yield and high stress tolerance obtained by screening provides a high-quality microbial resource for improving the quality of Maotai-flavor liquor.

A vegan yoghurt comprises a filamentous fungus and mesophilic bacteria and / or its residue. Preferably, the bacteria is live lactic-acid producing bacteria, e.g., mesophilic Lactococci or Lactobacilli,

The present invention relates to a dry sourdough composition and a method of making the dry sourdough composition. In particular, the present invention relates to a dry sourdough composition comprising dried and milled brewer's spent grains, wheat flour, one or more acid producing bacteria and one or more yeasts. Furthermore, the present invention relates to a method of making a dry sourdough composition comprising fermentation of a slurry of dried and milled brewer's spent grain, wheat flour, water, acid producing bacteria and yeast, followed by drying of the fermented slurry.

The invention belongs to the technical field of microorganisms, and particularly relates to an acid-producing strain for intestinal tracts of rocky mountain leafy monkeys and a separation and purification method of the acid-producing strain for the intestinal tracts of the rocky mountain leafy monkeys. The separation and purification method of the acid-producing strain comprises the following steps: (1) putting 0.1-0.6 g of monkey excrement into a centrifugal tube, adding 1-2 mL of sterile water, uniformly mixing in a vortex oscillator, taking 1000 mu L of turbid liquid by using a pipette, adding the turbid liquid into 50 mL of sterilized acid-producing bacterium enrichment culture medium, and culturing for 24 hours to obtain enriched bacterium liquid; the method comprises the following steps: 1) preparing a culture medium, 2) culturing with a bromocresol green culture medium, and 3) culturing with a CaCO3 selective culture medium, the strains B-5, B-13 and B-17 obtained through separation are smooth in surface, tidy in edge and thick in texture, acid tolerance test results show that when the pH value is 4.0 and 5.0, the survival rate of the strains is higher than 78%, and good acid tolerance is achieved.