66 results about "Acid producing bacteria" patented technology

The present invention relates to a method for preparing chitin and chitosan oligosac charide. The method comprises the steps that usual raw materials such as the crust of shrimp and crab, the insect crust or the fungal mycelia, etc. are micronized through the dry process or wet process; the carapace material of the obtained fine powder raw material is decalcified with the chemical process, and then is defatted and deproteinized with the method of micro-organism compound enzyme coarse enzyme liquid co-enzymolysis, and the insect and fungus fine powder thereof is directly defatted and deproteinized; a whole cell immobilizing bioreactor of a chitin deacetylase high-yield producing strain is prepared, to perform the circulatory deacetylation to the chitin and then obtain chitosan with corresponding degree of deacetylatoion; obligate anaerobic acid-producing bacterium and high-yield producing chitosan bacterium are utilized, the chitosan is submerged and fermented in the liquid, to obtain chitosan oligosaccharide with high water solubility. The present invention has the advantages that the method is helpful to fully utilize the resources, and makes the waste to the worth, at the same time, the default of the manufacturing process of the chemical process can be avoided, the production efficiency is improved, the energy is saved, the consumption is reduced, the byproduct with corresponding high value added can be produced, the comprehensive economic benefits of the relative secondary industry are obviously improved, the industrial development is promoted, and the multi-win effect is attained.

A mixture of probiotics effective to reduce the contamination of enteric bacteria in humans and other monogastric animals. The mixture of probiotics includes one or more acid-producing bacteria strains and one or more yeast strains, and may advantageously be supplemented with a source of nutrients, such as prebiotics including fructo-oligosaccharides. In a preferred embodiment, said one or more bacteria strains contain Enterococcus faecium strain NCIMB #10415, and said one or more yeast strains contain NCYC #47 and CNCM I-1079.

The present invention discloses a method for producing chitin/chitosan from the shell of silkworm chrysalis, fly larvina, which comprises: to break into pieces to progress the shell of silkworm chrysalis, fly larvina by dry or wet method; React the fine grits obtained with the fat enzyme and protein enzyme from micro-organism by a enzymolysis reaction in order to to eliminate the fat and protein contained in the chrysalis / larvina raw material; bleach using two-step method; Collect the materials after bleach and to wash, dry materials to obtain chitin product; Obtain chitosan by to circulate to cast off Acetyl reaction in a Rhizopus oryzae full cell immobilization biology reaction vessel; Obtain height hydrosoluble shel oligosaccharide by liquid submerged fermentation chitosan using acid formers. The present invention improves the quality of the product and the efficiency of production; reduces greatly the energy cost, the usage of acid, alkali, oxidant and other harmful chemistry thing and the discharge of effluent, waste residue; produces byproducts of high value at the same time; utilizes the resources integratedly; and increases the benefits of production.

The disclosure provides culture devices and methods useful for detecting acid-producing bacteria in a sample. The devices include a nutrient medium and a pH indicator to detect and differentiate acid-producing microorganisms, such as lactic acid bacteria. Methods of use include detecting or enumerating acid-producing microorganisms. The methods further provide for the detection of gas-producing acid-producing bacteria.

The invention relates to a sludge micro oxygen hydrolytic acidizing method, which includes: inoculating sludge containing acid-producing bacteria in a hydrolytic acidizing reactor, intermittently inputting air to the middle of the hydrolytic acidizing reactor for air stirring, simultaneously supplementing intermittent mechanical stirring, performing hydrolytic acidizing on sludge of a sewage treatment plant under micro oxygen conditions with dissolved oxygen (DO) kept to be 0.4-0.8 mg/L, leading the sludge after hydrolytic acidizing treatment to enter a settling pond for sludge-water separation, and leading liquid supernatant to serve as carbon sources for municipal sewage biological de-nitrification and dephosphorization treatment. The sludge micro oxygen hydrolytic acidizing method is conducted at normal temperature, is not needed to be added with acid and alkali for adjusting potential of hydrogen (pH) value, is simple in operation, stable in running and capable of restraining growth of methane bacteria and improving stability of a sludge hydrolytic acidizing system, and simultaneously leads sludge to obtain reduction and stabilization treatment to the maximum extent. Sludge acid yield is improved by 38%-50%.

The invention relates to a method which utilizes organisms of straws, chicken manure, cattle manure, pig manure, sheep manure and horse manure to mix and agitate with zymogeneous bacteria. The method comprises the following steps: firstly filling the organisms in a ground pool, covering the upper part of the organisms for 3 to 5 days by using a plastic film; then refilling the organisms in a marsh gas generator after the temperature is up to 60 DEG C; adding 1 to 2 percent of limewater, so as to neutralize organic acid generated; controlling the pH value to be within 6.8 to 7.5, so that acid-producing bacteria and methane bacteria are subjected to the dry fermentation of anaerobic exudate and marsh gas; after the speed is balanced, generating anaerobic bacteria, and generating mixed gas; and generating pure march gas after the mixed gas is desulfurized, dehydrated and purified. The gas production amount of the organisms per kilogram averagely reach 0.35m<3>, the gas production rate averagely is 0.398m<3>. The method is widely applied to cooking and illuminating in a family.

The invention discloses a composite coating liquid of fibroin and a preparation method and an application thereof. The composite coating liquid of fibroin is composed of the following components: 2 to 10 wt percent of crosslinking agent, 0.5 to 2 wt percent of acetate acid, 10 to 20 percent of fibroin and water which accounts for the rest weight proportion. The preparation method of the invention comprises the steps of (1) the preparation of the fibroin and (2) the preparation of the composite coating liquid of fibroin. The fibroin of the invention has the functions of naturally moisturizing the skin, antisepsis, antianaphylaxis, etc. and is applicable to the preparation of lotions for women, infants or children; thus achieving the purposes of moistening the skin, absorbing moisture, resisting bacteria and treating and preventing skin allergy; the product prepared by the invention has significant functions of moistening the skin, absorbing moisture, venting, preventing skin allergy and inhibiting bacteria, especially has remarkable inhibited effect on pathogenic anaerobion and acid-producing bacteria.

The invention relates to an organic waste water anaerobic treatment method, which belongs to the waste water treatment technology field. The invention adopts a processing apparatus composed by a tower type container with and a water distribution device, a three phase separator and a matched gas-liquid pressure balancer doubled as a water power flame arrester disposed therein; an acid-producing bacteria gathering area and a methane-producing bacteria gathering area are disposed above the water distribution device. the processed water is formed into homogeneously distributed rising water flow on the cross section of the apparatus by passing through the water distribution device; the organic compounds in the water is decomposed into methane and carbon dioxide gas in the acid-producing bacteria gathering area and the methane-producing bacteria gathering area; all the gases and liquid are passed through the three phase separator to be separated and the gas-liquid pressure balancer doubled as a water power flame arrester to be discharged; and the anaerobic bacteria sludge is entrapped in the apparatus effectively without loss. The invention needs not to dispose hydrolysis-acidification pool or water outlet backflow, has a simple process, can operate stably under the volume load of 1-30kgCOD/M.d, and can reach the organic compounds removal rate of waste water to about 90%.

The invention discloses a technological method of the parallel-connected sequencing batch dry type anaerobic digestion treatment of organic solid wastes, belonging to the technical field of environmental protection. Four sequencing batch dry type anaerobic digestion reaction pots are in parallel connected; each sequencing batch dry type anaerobic digestion reaction pot is correspondingly provided with a biogas slurry collection pool, i.e. the four sequencing batch dry type anaerobic digestion reaction pots (A, B, C, D) respectively correspond to four biogas slurry collection pools (a, b, c, d); and each biogas slurry collection pool is connected with a submerged pump and a valve. Biogas slurry enters a main biogas slurry regurgitated pipe through the submerged pump, enters the corresponding main biogas slurry regurgitated pipe by opening the corresponding valve according to the digestion process of materials and is uniformly sprayed to the materials through a spraying device. The invention forms different technological conditions to furthest activate the activity of two anaerobic nitrifying bacteria to accelerate the degradation of the organic solid wastes and enhance the aerogenesis speed by utilizing the difference of optimal growing environments of acid-producing bacteria and methanogens through the recharge of the biogas slurry.

The invention belongs to the field of feed production, and particularly relates to a fermented biological feed and a preparation method thereof. The preparation method of the fermented biological feed comprises the following steps: respectively carrying out strain rejuvenation activation and primary propagation on amylase-producing bacteria, proteinase-producing bacteria and acid-producing bacteria to obtain an amylase-producing bacteria solution, a proteinase-producing bacteria solution and an acid-producing bacteria solution; proportionally inoculating the amylase-producing bacteria solution, proteinase-producing bacteria solution and acid-producing bacteria solution into a co-culture medium, and carrying out co-culture to obtain a mixed bacteria solution; and adding the mixed bacteria solution and water into a fermentation raw material, uniformly mixing, carrying out trough fermentation, and obtaining the fermented biological feed after the fermentation finishes. The fermented biological feed is partially substituted for the traditional animal feed or animal feed additive, is capable of effectively enhancing the feed nutritive value and utilization ratio, improving the problem in intestinal tracts in livestock/poultry breeding, enhancing the disease resistance of the bred animals, and reduces the use of antibiotic drugs.

The invention relates to a method for preparing D-tagatose and L-tagatose from dulcitol in the technical field of biology, which comprises the following steps: preparing a culture medium; inoculating acid-producing bacteria into the culture medium to prepare primary seed liquid and secondary seed liquid; culturing the second seed liquid to obtain a water solution containing the D-tagatose and the L-tagatose. The invention also relates to a preparation method adopting resting cell technology, which comprises the following steps: inoculating the acid-producing bacteria into the culture medium to be cultured; centrifugating the cultured bacteria after finishing the to form somatic cells, suspending the somatic cells by using the water solution containing the D-tagatose, and culturing the water solution to obtain the water solution containing the D-tagatose and the L-tagatose. The invention also relates to a preparation method adopting immobilized cell technology, which comprises the following steps: inoculating the acid-producing bacteria into the culture medium to be cultured; centrifugating the cultured bacteria after finishing the culture to obtain somatic cells; obtaining the water solution containing the D-tagatose and the L-tagatose by adopting the immobilized cell technology. The methods of the invention are green and safe, have high conversion efficiency, and can prepare the D-tagatose and the L-tagatose of pharmaceutical grade.

The invention relates to biological fertilizer and a preparation method and a use method thereof. Acid producing bacteria are added into organic fertilizer to obtain the biological organic fertilizer.Through the addition of the acid producing bacteria, organic matters can be fast decomposed; the pathogenic bacterium breeding and crop disease occurrence can be reduced; the crop yield is improved.

The present invention is directed to a process for producing a dairy flavour ingredient, with an intense dairy flavour and low pH especially suitable for flavouring dairy products which are produced by “rapid” dairy technologies. The process includes blending a dairy based protein with an acid producing producing bacteria, fermenting at ph<5.5, further blending with ground cheese, further fermentation with and enzyme and heat inactivating the enzyme.

The invention discloses a method for anaerobic synthesis of volatile fatty acid by using wood carbon source sewage mixed bacteria. The method comprises the following steps of (1) performing hydrolyzing and enzymolysis on a xyloid biological resource by a high-temperature high-pressure hydrothermal pretreatment method; (2) using wood hydrolysis-enzymatic hydrolysate (reducing sugar) as a carbon source, performing anaerobic domesticizing on the sewage containing activated sludge, and performing enriching to synthetize acid-producing bacteria of volatile fatty acid; and (3) performing anaerobic fermentation on domesticized sewage to generate acid, through regulating factors of the concentration of the reducing sugar, pH value, fermentation temperature, the number of fermentation days and thelike, increasing the yield of the volatile fatty acid, finally, determining the volatile fatty acid as mixed acid of acetic acid, propanoic acid and butyric acid. Through contrast of total amount of the volatile fatty acid and the proportion of the components namely acid in various experiment groups, the optimal acid generation condition can be obtained. By the method, the purpose of synthetizingthe volatile fatty acid with low-price carbon sources can be realized, the best acid generating time is greatly shortened, and the energy consumption and the cost can be saved.

The invention relates to methods for preparing L-tagatose, which belongs to the field of biotechnology. A method for preparing the L-tagatose comprises the following steps: preparing a culture medium; culturing acid-producing bacteria to obtain primary seed liquid; obtaining secondary seed liquid; inoculating secondary seed liquid to a fermentation culture medium to obtain fermentation liquor; obtaining primary yeast seed liquid and secondary yeast seed liquid and centrifuging the primary yeast seed liquid and the secondary yeast seed liquid to obtain yeast cells; culturing the yeast cells to obtain clear fermentation liquor; concentrating, decolorizing and filtering the clear fermentation liquor to obtain solution; and passing the solution through an ion exchange resin, performing crystallization, washing crystals and drying the crystals to obtain the L-tagatose. A method for preparing the L-tagatose by converting D-dulcitol by using resting cells comprises the following steps: preparing a culture medium; culturing bacteria; suspending somatic cells of the bacteria to obtain solution; inoculating candida ssp. CGMCC No.3268 to obtain fermentation liquor; and subjecting the fermentation liquor to decolorizing and desalting processing to obtain aqueous solution, concentrating the aqueous, performing crystallization, washing crystals and drying the crystals to obtain the L-tagatose. The methods of the invention can be used for preparing pharmaceutical L-tagatose.

The invention provides Baijiu capable of strengthening bodies and a preparation method thereof, and is characterized in that the Baijiu is prepared from the following raw materials in parts by weight: 400-450 parts of waste distiller's grains of yellow wine, 100-110 parts of rice, 100-110 parts of rice husks, 10-11 parts of broomcorn millet, 10-11 parts of orange peel, 3-4 parts of radix salviae miltiorrhizae, 2-3 parts of schisandra chinensis, 1-2 parts of cordyceps sinensis, 3-4 parts of white paeony root, 25-30 parts of Daqu, 0.5-1 part of tea polyphenol, and 0.5-0.7 part of xylooligosaccharide. The waste distiller's grains of the yellow wine are adopted as the raw material to produce the Baijiu, so that a production link of a yellow wine factory is extended, and the produced Baijiu is mellow and full and is relatively long in aftertaste; the rice husks are subjected to puffing treatment, so that the raw materials are looser; the tea polyphenol having selective bacteriostasis is added, has an inhibitory effect on acid-producing bacteria lactobacillus, has a role of maintaining the bacterial colony balance on mould and other fungi, and avoids fast acid rising of easy-fermentation materials during accumulation and fermentation; the added xylooligosaccharide can protect liver; and in addition, the Baijiu can strengthen the bodies.

The invention discloses a hydrolytic acidification complex microbial inoculant for water treatment and a preparation method thereof. The hydrolytic acidification complex microbial inoculant comprisesa composite microbial flora and nutrient substances. The composite microbial flora is composed of clostridium butyricum, bacillus subtilis, yeast, white rot fungi, rhodopseudomonas, lactobacillus acidophilus, denitrifying bacillus, acinetobacter, lampropedia and thiobacillus denitrificans, and the strains are obtained by a commercial approach. The invention relates to the water treatment field. According to the hydrolytic acidification complex microbial inoculant for water treatment and the preparation method thereof, high-efficiency acid-producing bacteria, high-efficiency hydrolytic bacteriaand water treatment functional bacteria can be reasonably collocated without mutual antagonism, the bioactivity is high, the bacteria content is high, the complex microbial inoculant has rapid propagation speed, can be added into a sewage treatment system according to certain amount, and can enrich the variety and abundance of microorganisms in a hydrolytic acidification tank, and improve the treatment efficiency and operating stability of the biochemical system. The complex microbial inoculant is suitable for the hydrolytic acidification process section of water treatment, and can enhance the capacity and quality of water treatment and lower the operating cost.

The invention discloses a compound preparation capable of effectively improving the disease resistance of aquatic animals and a preparation method thereof. The compound preparation comprises the following components in parts by mass: 55-65 parts of microbial powder, 10-15 parts of astragalus polysaccharide, 10-15 parts of yeast cell walls, 5-10 parts of plant essential oil and 1-5 parts of vitaminB6. The coordination of the components is capable of reforming the intestinal flora structure, changing the proportion of unsaturated fatty acids in metabolic products of intestinal acid-producing bacteria, inhibiting enteric pathogenic bacteria and other harmful substances, restoring intestinal mucous membranes, promoting digestion and absorption of nutrients, simultaneously enabling animal bodies to have enhanced nonspecific immunity, enhanced stress resistance and stronger resistivity to viruses, pathogenic bacteria, parasites and polluted environments, comprehensively improving the utilization ratio of feed and survival rate of the animal bodies, reducing residual feed, purifying excrement and optimizing and normalizing an intensive culture mode.

The invention provides a subtropical zone karst region degenerated site vegetation recovery soil restoration material, and a production method thereof. The subtropical zone karst region degenerated site vegetation recovery soil restoration material is produced through mixing fermenting of acid producing microorganism with an organic-inorganic mixed material with certain fertilizer and water retention functions for a certain period of time. Loading of the acid producing bacteria is adopted, so that organic acid substances produced by the acid producing bacteria possess a plurality of active groups including carboxyl group, hydroxyl group, alcoholic hydroxyl group, sulfonic group, and amino acid group; and weak acidity, colloid adsorption performance, ion exchange performance, complexing performance, chelating performance, and surface activity are achieved; the subtropical zone karst region degenerated site vegetation recovery soil restoration material is capable of reacting with carbonates, degrading carbonates and releasing nutrients immobilized in the carbonates for using by plants and microorganisms; according to applications of the subtropical zone karst region degenerated sitevegetation recovery soil restoration material, organic acids generated in adhered microorganisms in growth process are capable of reducing soil pH value, increasing soil porosity, and improving soil physical and chemical performance; the subtropical zone karst region degenerated site vegetation recovery soil restoration material also possesses relatively excellent water retention and fertilizer conservation functions, and is capable of promoting vegetation recovery effectively.

The invention discloses a method for treating wastewater produced during extraction of saponin from dioscorea zingiberensis. The method comprises steps as follows: (1), pretreatment: H<+> in the wastewater is sequentially neutralized with limestone and lime milk, and colloids, suspended solids and sulfate are removed; (2) two-stage anaerobic treatment: at the first stage of anaerobic treatment, the pretreated wastewater is subjected to hydrolytic acidification through hydrolytic acid-producing bacteria, macromolecular organic compounds are decomposed to be micromolecular organic compounds, and insoluble organic matters are hydrolyzed to form soluble organic matters; at the second stage of anaerobic treatment, an acidized product is further decomposed through methanogenic bacteria to generate methane, CO2 and H2O; (3) aerobiotic biochemical treatment: organic pollutants in anaerobic effluent are further decomposed under the action of activated sludge formed through air and aerobic microorganisms; (4) advanced treatment: aerobic effluent is treated under the synergistic effect of ozone and activated carbon. Treatment process units with different functions are reasonably combined and are successfully applied to treatment of produced wastewater dioscorea zingiberensis saponin.

The invention provides a rural biological waste carbon-bottom dual pit and a composting treatment method. The carbon-bottom dual pit is characterized by being a deep pit excavated below a ground surface; a carbon bottom is arranged at the bottom of the deep pit; the carbon bottom is prepared by mixing 35 to 45 percent of biomass carbon, 20 to 30 percent of lime, 20 to 25 percent of cement and 5 to 10 percent of wood tar oil to form the pit bottom, and is compacted; a partition plate is arranged in the deep pit, and perpendicularly divides the deep pit into at least two pits; one deep pit is excavated at a low-lying position, or multiple deep pits are excavated in a high-to-low flowing direction; the carbon bottom is arranged at the bottom of each deep pit; solid-liquid wastes fed into the deep pit comprise biological wastes, animal manure and the like; after being filled with the wastes, the first deep pit is covered with a wood board cover for composting, and then the second deep pit starts to be used; 1 to 2 percent of saccharomycetes and 1 to 2 percent of malt acid-producing bacteria are added into the deep pits to accelerate composting; the wastes are dug out and returned to fields, are used as a special fertilizer and can be recycled; the biological wastes are composted, so that the problem of accumulation of the rural biological wastes is solved; the countryside ecological environment is improved; the living quality and the health of countryside inhabitants are improved.

A method for rapidly screening silicate bacteria comprises the following steps: (1) obtaining a soil bacterium suspension; (2) performing enrichment culture, namely employing a method of gradually improving the addition amount of bauxite to perform enrichment culture on silicate bacteria; (3) performing screening culture, namely selecting a mucus-shaped raised transparent bacterium colony on a culture medium plate according to bacterium colony growing time and bacterium colony form, and performing once-more culture until a pure culture is obtained; and (4) performing secondary screening. By employing the method of gradually improving the addition amount of bauxite, bacterial relatively easily adapt to silicate environment, and thus more silicate bacteria are enriched. By employing the method of smearing CaCO3 powder on plate surface for enabling CaCO3 to not be precipitated to the bottom of the plate, screening of acid-producing bacteria is visualized. By replacing solid FeCl3 added into the culture medium in a trace amount with a FeCl3 solution, the FeCl3 solution is relatively easy to utilize by bacteria. The method is capable of rapidly conveniently screening and domesticating silicate bacteria in a laboratory.

The invention discloses a heating type integrated sewage treatment device. In the sewage treatment device, a reactor (1) is divided to a hydrolysis-acidification tank (1a), a methane-producing tank (1b) and a transition zone (1c) by baffles (6) which are arranged longitudinally, wherein the upper part of the hydrolysis-acidification tank (1a) is communicated with the lower part of the methane-producing tank (1b) through the transition zone (1c); and the sewage treatment device also comprises a mixer (7) and a solar heat transfer system (8). By adopting the device disclosed by the invention, hydrolysis-acidification and methane producing are combined in a reactor; the transition zone is arranged between the hydrolysis-acidification tank and the methane-producing tank to ensure that sewage can flow into the lower part of the methane-producing tank from top to bottom; and the solar heat transfer system is combined, thus the sewage temperature can be increased, the mass transfer effect between sludge and water can be increased, and the device is good for acid-producing bacteria and methanogens and is very energy-saving and environmentally friendly.

The invention relates to preparation of response release materials, in particular to a dual-response release sterilization material based on mixed wrapping of gelatin and Cu-BTA complex and a preparation method thereof. Halloysite nanotubes and a gelatin solution with a dispersed complex are mixed and subjected to cross-linking treatment under the action of a cross-linking agent, and the precipitate is collected, thus obtaining a complex mixed wrapping material. The release material prepared according to the invention makes use of the sulfur ions and hydrogen ions released by common sulfate reducing bacteria and some acid-producing bacteria in a metabolic process in the environment as signal molecules, and based on the point, the dual-response release material based on mixed wrapping of gelatin and Cu-BTA complex can be obtained. The principle of response release lies in that sulfur ions can take away Cu ions to form CuS precipitate, thus destroying a Cu-BTA film layer in a mixture wrapping layer; under the acidic condition, a gelatin film layer can be protonated, thus avoiding rejecting protonated halloysite nanotubes, and when the gelatin layer is dissociated, a bactericide is released. The preparation method for constructing the drug loading system is simple, and has strong pertinence.