Detection of acid-producing bacteria

A technology of acid-producing bacteria and culture medium, which is applied in the fields of body and surface, and can solve problems such as consumption

Active Publication Date: 2012-11-28
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods can take up to four days to detect or identify acid producing bacteri

Method used

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  • Detection of acid-producing bacteria
  • Detection of acid-producing bacteria
  • Detection of acid-producing bacteria

Examples

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preparation example Construction

[0125] Kits of the invention may additionally include sample preparation accessories to aid in sample preparation and / or inoculation. Non-limiting examples of sample preparation accessories include diluents, sample acquisition devices (eg, swabs, sponges, spatulas), and pipettes.

[0126] The invention will now be further illustrated with reference to the following non-limiting examples. All parts and percentages are expressed by weight unless otherwise indicated.

example 1

[0129] Detection of Lactic Acid Bacteria Isolated from Processed Meat Samples Using Bromocresol Purple pH Indicator

[0130] 3M PETRIFILM Aerobic Count plate was obtained from 3M Company (St. Paul, MN). DIFCO MRS Broth was obtained from BD-Diagnostic Systems (Sparks, MD).

[0131] Four strains of lactic acid producing bacteria were isolated from processed meat products. Two species of this culture were identified as Leuconostoc mesenteroides and Weisella viridescens, Lactococcus lactis subspecies lactis and Streptococcus oralis.

[0132] Bromocresol Purple (BCP) pH indicator was obtained from Eastman Kodak (Rochester, NY). Chlorophenol red (CPR) (CAS NO. 4430-20-0) was obtained from Sigma-Aldrich (St. Louis). The pH indicators were added separately to the MRS broth to achieve a final concentration of 0.5 mM BCP or CPR. After dissolving the pH indicator, the solution is sterilized by filtration.

[0133] Bacterial cultures were prepared by inoculating pure cultures onto ...

example 2

[0135] Detection of Lactic Acid Bacteria Isolated from Processed Meat Samples Using Chlorophenol Red pH Indicator

[0136] A concentrated solution of sterile chlorophenol red was added to the MRS dilution to a final concentration of 0.21 g / L (0.5 mM). Lactic acid bacteria cultures were prepared and diluted into the MRS / chlorophenol red solution as described in Example 1, except that Streptococcus oralis was not tested in this experiment. The diluted bacterial suspension was used to inoculate PETRIFILM Aerobic Microbial Counting Plates as described in Example 1. The plates were incubated at 30°C and visually inspected for signs of bacterial growth. The results are shown in Table 3.

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Abstract

The disclosure provides culture devices and methods useful for detecting acid-producing bacteria in a sample. The devices include a nutrient medium and a pH indicator to detect and differentiate acid-producing microorganisms, such as lactic acid bacteria. Methods of use include detecting or enumerating acid-producing microorganisms. The methods further provide for the detection of gas-producing acid-producing bacteria.

Description

[0001] Cross references to related patent applications [0002] This patent application claims the benefit of U.S. Provisional Patent Application No. 61 / 187,107, filed June 15, 2009, and U.S. Provisional Patent Application No. 61 / 314,140, ​​filed March 15, 2010, combining both provisional patent applications The application is hereby incorporated by reference in its entirety. Background technique [0003] Acidogens comprise a relatively diverse group of Gram-positive microorganisms that share common metabolic and physiological characteristics. This group of bacteria produces acid as the main end product of carbohydrate fermentation. This group is divided into two metabolic subgroups - homolactic fermenters, which essentially convert carbohydrates into acids; and heterolactic fermenters, which, in addition to producing acids, also convert carbohydrates into other metabolites, including e.g. ethanol and carbon dioxide. [0004] Although some acidogenic bacteria such as lacti...

Claims

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Application Information

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IPC IPC(8): C12Q1/04G01N33/84
CPCC12Q1/04C12M23/04C12M23/20C12M23/34C12M25/06C12M41/36
Inventor 罗伯特·F·杨帕特里克·A·马赫迈克尔·E·休吉斯克里斯蒂娜·A·宾斯费尔德贾森·W·比约克玛拉·S·赖夫-温纳亨利·J·吕布朗特
Owner 3M INNOVATIVE PROPERTIES CO
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