The invention relates to an
animal origin-free low-
protein culture medium suitable for animal
cell product production, comprising 24 basic
metabolism nutrients, 11 vitamins, 3
transferrin substitute compounds, 5 lipid compounds, 2
nucleic acid compounds, 4 hormones and growth factors, 3 antioxidants, 1 shear-resistant protective agent, 1
pH indicator, 2 pH buffers, 9 other
inorganic salts, soy hydrolysates adopted to substitute
animal origin component, and composition of
ferrous sulfate,
ferric nitrate and EDTA-2Na adopted to substitute
transferrin. The culture medium can be made by dissolving the aforementioned components in triply
distilled water. The positive effects of the culture medium are as follows: the culture medium contains no
animal origin component, the
total protein content is lower than 10mg / L, which helps separate and purify products and is suitable for production of recombinant
protein medicaments; the culture medium supports
normal growth and long-term subculturing of animal cells; the culture medium can be used without
adaptation, is easily prepared and is suitable for massive production of animal
cell products.