The invention provides a method for sterile
sowing and efficient propagation of
cymbidium faberi. The method comprises the following steps: pretreatment of
cymbidium faberi seeds, sterile
germination of the
cymbidium faberi seeds, protocorm proliferation, differentiation,
seedling strengthening and
acclimatization and
transplantation. The pretreatment comprises the steps of refrigerating
hybrid seeds of cymbidium orchid at 4 DEG C, performing secondary disinfection with
sodium hypochlorite, performing dark culture for 10-30 days after
sowing, and then performing light culture, and the
germination culture medium is prepared by taking 1 / 4 MS as a basic culture medium and adding 100-200ml / L of coconut water, 0.5-1.0 mg / L of 6-benayl
aminopurine, 0.1-0.5 mg / L of
naphthylacetic acid, 0.5-2.0 g / L of peptone and 0.5-1.0 g / L of
activated carbon. According to the method, the seed
germination rate can be effectively increased, the 60d seed germination rate reaches up to 85%, the
pollution rate is reduced to 5% or below, 50.0 g / L
inositol, 2.0 mg / L
glycine and 2.0 mg / L nicotinic acid are added into a differentiation culture formula, the differentiation rate can be effectively increased, the leaf
bud differentiation rate reaches up to 90%, meanwhile, rooting can be induced, the
growth time of
cymbidium faberi from seeds to seedlings is greatly shortened, a large number of high-quality seedlings can be obtained within a short time, and the method is suitable for large-scale popularization and application. Technical data are accumulated for cymbidium
resource protection and new variety cultivation.