277 results about "Arabinose" patented technology

This invention provides an in vitro cell-free protein synthesis system, its reagent kit, and its applications, belonging to the field of protein synthesis technology. This invention improves an in vitro cell-free protein synthesis system based on Kluyveromyces lactis by adding exogenous L-arabinose, significantly enhancing the system's protein synthesis capacity. Furthermore, it provides a more efficient and higher-throughput in vitro protein synthesis reagent kit and a method for synthesizing exogenous proteins. The improvements provided by this invention can be achieved without molecular modification, are simple and convenient to operate, and save costs.

The utility model belongs to the technical field of sugar alcohol preparation, and relates to a system for co-producing L-arabinose and caramel pigment by using xylose chromatography raffinate, which comprises a raw material tank, a nanofiltration membrane separator, a chromatography separation device, an evaporation concentration tank, a crystallization tank, a centrifugal separator and a dryer which are sequentially communicated by pipelines, and the browning reaction assembly is used for mixing the raffinate and the centrifugal mother liquor and then carrying out browning reaction treatment to prepare caramel pigment. The chromatographic separation device is used for separating an extracting solution and raffinate from the flowing nanofiltration permeate, the evaporation concentration tank and the crystallizing tank are used for concentrating and crystallizing the flowing extracting solution into massecuite, the centrifugal separator is used for separating the flowing massecuite into moist sugar and centrifugal mother liquor, and the dryer is used for drying the flowing moist sugar into L-arabinose crystals. According to the utility model, raffinate and centrifugal mother liquor are mixed to prepare a caramel pigment liquid product, so that a caramel pigment additional product is obtained while the utilization rate of saccharide resources is improved.

The application provides a Huangdache oligosaccharide with GLP-1 agonist activity and application thereof, and belongs to the technical field of medicines. 3 The oligosaccharide component ELYP-3 has a molecular weight of 3.2*10 3 Da, and the molar ratio of monosaccharides is rhamnose:galacturonic acid:glucose:galactose:arabinose = 1:32.42:45.37:9.11:6.54. The Huangdache oligosaccharide fragment obtained by glycosidase degradation has significant hypoglycemic and weight loss activities, the preparation process is simple, specific and green, and provides scientific basis and theoretical support for the development of natural GLP-1 agonists. Meanwhile, the functional oligosaccharide provides a wide application prospect for the development of medicines with blood glucose regulation.

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention discloses application of uncaria acidic polysaccharide URP-2 in preparation of an anti-esophageal cancer drug, and belongs to the technical field of antitumor of medicinal plant polysaccharide. According to the method, a method for extracting the uncaria total polysaccharide by boiling water and precipitating the uncaria total polysaccharide by ethanol is adopted, and a DEAE DE-52 cellulose column and sephadex column purification technology is combined, so that the separation of the uncaria acidic polysaccharide component is realized, and the uncaria acidic polysaccharide URP-2 is obtained. The uncaria acidic polysaccharide URP-2 comprises the following components in mole percent: 14.11% of arabinose, 12.64% of rhamnose, 6.48% of galactose, 5.00% of glucose and 61.77% of galacturonic acid. Experiments show that the uncaria acidic polysaccharide URP-2 has remarkable anti-esophageal cancer activity, so that a potential experimental basis and a new strategy are provided for applying the uncaria acidic polysaccharide URP-2 to clinical treatment of esophageal cancer.

The invention discloses a preparation method and application of roasted barley and malt polysaccharide with an anti-cancer effect. The roasted barley malt polysaccharide is a neutral heteropolysaccharide which mainly takes-> 3)-beta-D-Glcp-(1-> and->-beta-D-Glcp-(1->) as main chains, branches exist at O-3 / O-4 positions, and the branch chains are mainly composed of-> 4)-beta-D-Xylp-(1->,-> 3, 4)-beta-D-Xylp-(1-> and alpha-D-Glcp-(1->. The monosaccharide composition comprises arabinose, glucose and xylose in a molar mass ratio of 1.86: 33.43: 1.00, and the molecular weight is 23.81 kDa. In-vitro activity experiments prove that the roasted barley malt polysaccharide disclosed by the invention has a remarkable inhibition effect on proliferation of liver cancer cells, lung cancer cells, breast cancer cells and colorectal cancer cells, particularly on the liver cancer cells, and shows outstanding anti-tumor activity, so that the roasted barley malt polysaccharide is expected to be developed and applied in the fields of foods, health-care products or medicines.

The invention relates to the technical field of plant extraction, in particular to a multi-product preparation method based on an arabinoxylan-lignin compound. The method comprises the following steps: treating a lignocellulose raw material by adopting a weak base extracting solution and a strong base extracting solution, and separating an obtained strong base extracting and dealkalizing suspension into an arabinoxylan-lignin compound dispersion liquid and arabinoxylan; performing enzymolysis on the dispersion liquid, and filtering enzymatic hydrolysate to obtain a precipitate which is lignin and a filtrate which is xylooligosaccharide liquid; and separating and purifying the xylooligosaccharide liquid to obtain xylooligosaccharide, xylose and arabinose. According to the method, on the basis of a weak base-strong base two-step extraction process, obtained arabinoxylan-lignin compound dispersion liquid is subjected to enzymolysis, so that lignin, xylooligosaccharide, xylose and arabinose can be continuously prepared, and the reutilization of the arabinoxylan-lignin compound is realized.

The invention discloses a composition for regulating body weight through satiety-sugar control double channels. The composition is prepared from the following components in parts by weight: 5-25 parts of cinnamon extract, 1-20 parts of ganoderma lucidum EGCG composite powder, 10-40 parts of L-arabinose, 5-40 parts of composite fruit and vegetable fiber powder, 5-25 parts of fructus cannabis protein peptide, 1-20 parts of apple polyphenol, 1-20 parts of white kidney bean dietary fiber powder and 1-20 parts of agaricus bisporus powder. The composition is characterized in that digestion and absorption of carbohydrate are inhibited, the swelling ratio larger than or equal to 35 times is achieved through a composite fiber system, gastric emptying is remarkably prolonged, satiety hormones such as GLP-1 are adjusted, and the composition can be used as a non-alternative weight management product and is good in safety.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention relates to the technical field of plant polysaccharides, and particularly discloses a red-knees herb polysaccharide and a preparation method and application thereof, the peak top molecular weight of the red-knees herb polysaccharide is 33.9 KDa, the weight-average molecular weight of the red-knees herb polysaccharide is 42.8 KDa, the number-average molecular weight of the red-knees herb polysaccharide is 10.0 KDa, and the red-knees herb polysaccharide is mainly composed of galacturonic acid, galactose, arabinose, rhamnose and glucuronic acid. According to the preparation method of the red-knees herb polysaccharide, by optimizing process parameters and controlling the reasonable solid-liquid ratio, heating temperature and heating time, the dissolution rate of the polysaccharide is increased, the dissolution rate of impurities is reduced, and the yield of the red-knees herb polysaccharide is increased. The red-knees herb polysaccharide is used as a raw material, ethanol is used for alcohol precipitation, polysaccharide components are precipitated, impurities are preliminarily removed, then macroporous resin is used for purification, the recovery rate of polysaccharide and the removal rate of impurities such as pigment and protein are improved by screening optimization and pretreatment of the macroporous resin, and the total sugar content of the purified red-knees herb polysaccharide is 80% or above. Use of toxic chemical reagents is avoided, and the preparation method is safe and efficient.

The invention discloses arabinose glycosyl transferase PnUGT73B4 and application thereof to preparation of panax japonicus saponin IV, the amino acid sequence of the arabinose glycosyl transferase is shown as SEQ ID NO: 1, under the catalysis of the arabinose glycosyl transferase PnUGT73B4, UDP-arabinose is added to a glucuronic acid group on the C3 site of panax japonicus saponin IVa, and the panax japonicus saponin IV is generated; according to the method, the requirement for raw material plants can be reduced, agricultural land is saved, the product is single, preparation of the panax japonicus saponin IV is facilitated, the biosynthesis route of oleanane type triterpenoid saponin is analyzed, and a breakthrough solution is provided for biosynthesis of the panax japonicus saponin IV.

The invention discloses purified barley seedling polysaccharide as well as a preparation method and application thereof. The polysaccharide is prepared from six monosaccharides, namely rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 2.88: 3.39: 1.51: 1: 0.76: 3.2. The relative molecular weight is 254.09 kDa. A fermentation method is combined with an innovative purification process, so that the barley seedling polysaccharide monomer with high purity and uniform structure is obtained for the first time. Through a multi-dimensional characterization technology, fine structure characteristics such as monosaccharide composition, glucosidic bond connection mode and branching degree are systematically analyzed, and a reliable material basis is provided for establishing structure-function accurate association. The purified barley seedling polysaccharide has a remarkable effect of promoting intestinal peristalsis, not only lays a scientific foundation for deep processing of barley seedlings and research and development of related functional products, but also provides a new theoretical support for relieving intestinal peristalsis disorder, and has a wide application prospect.

The invention discloses a composite plant polypeptide composition with the effects of repairing pancreas and reducing blood sugar. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of ginsenoside, 5 to 30 parts of radix astragali seu hedysari extract, 3 to 20 parts of radix rehmanniae polysaccharide, 2 to 15 parts of radix trichosanthis alkaloid, 5 to 25 parts of rhizoma dioscoreae polysaccharide, 5 to 30 parts of bitter gourd polypeptide, 3 to 20 parts of folium mori alkaloid, 8 to 40 parts of rhizoma polygonati polysaccharide, 5 to 25 parts of fructus hippophae flavone, 10 to 35 parts of wheat germ peptide, 2 to 15 parts of coptis berberine, 10 to 45 parts of corn stigma polysaccharide and 3 to 20 parts of saussurea involucrata polyphenol. 1-10 parts of sophora flavescens total alkaloids, 5-25 parts of a dendrobium extract, 5-30 parts of a radix puerariae extract, 8-35 parts of L-arabinose and 10-40 parts of small molecule peptides; 20 to 100 parts of collagen peptide and 15 to 80 parts of albumin peptide; 5-50 parts of microcrystalline cellulose, 3-30 parts of fructo-oligosaccharide and 0.5-5 parts of magnesium stearate; the limitation of single sugar control or simple component mixing in the prior art is broken through, a synergistic system of plant active components and functional polypeptides is constructed, and the purity and retention rate of the plant active components are improved by optimizing the extraction process.

The invention discloses neutral homogeneous lycium barbarum polysaccharide as well as a preparation method and application thereof. Through systematic structure characterization, it is clarified for the first time that the polysaccharide NLBPE1 is atypical arabinogalactan with 1, 5-alpha-L-arabinose as a bridging unit, and the polysaccharide NLBPE1 has a uniform molecular weight and a definite branched chain topological structure. The NLBPE1 serving as an adjuvant and an antigen are jointly entrapped in various delivery systems (such as PLGA nanoparticles, lipidosome, hydrogel and the like) to form the vaccine composition, so that the immunogenicity of the vaccine can be remarkably improved, and the antigen-specific T cell response can be enhanced. The invention provides an application scheme of the polysaccharide as a vaccine adjuvant in tumor immunotherapy and tumor prevention, and particularly shows a remarkable synergistic anticancer effect when the polysaccharide is combined with an immune checkpoint inhibitor for use. The polysaccharide has a clear structure, good biocompatibility and remarkable immunological enhancement activity, and has a wide application prospect of tumor vaccine adjuvants.

The invention provides application of pitaya peel polysaccharide in preparation of a medicine for repairing skin wounds. The monosaccharide components of the pitaya peel polysaccharide comprise rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. Research finds that the dragon fruit peel polysaccharide remarkably accelerates the wound healing process and promotes granulation tissue formation and collagen deposition. Meanwhile, the pitaya peel polysaccharide can also promote cell proliferation and neovascularization at the skin wound part, exert an anti-inflammatory effect, remodel skin microbiota and provide a more suitable microenvironment for skin wound healing. The invention provides a new drug choice for clinically repairing skin wounds, and can be used for developing new skin wound dressings.

The invention belongs to the technical field of medicine and health care, and discloses oleander oligosaccharide and a preparation method and application thereof, the molecular weight of the oleander oligosaccharide is 2900 Da, and the oleander oligosaccharide is composed of glucose, mannose, galactose and arabinose; the preparation method comprises the following steps: degreasing oleander roots with ethanol, respectively carrying out water extraction and gradient concentration alcohol extraction, and then sequentially carrying out ceramic membrane-ultrafiltration-nanofiltration enrichment, ion exchange column chromatography and molecular sieve gel column purification on the alcohol extract to obtain the oleander extract. The oligosaccharide can be applied to preparation of immunological enhancement drugs, corresponding tumor immune drugs or health care products. The preparation method is efficient and safe, and the prepared product is good in quality and can be applied to preparation of immunological enhancement drugs, corresponding tumor immune drugs or health care products.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

This invention discloses an arabinogalactan sulfate derived from *Streptococcus filamentosa*, its preparation method, and its applications, belonging to the fields of natural product chemistry and marine biological resource development technology. The technical solution includes an arabinogalactan sulfate derived from *Streptococcus filamentosa*, with a monosaccharide composition comprising arabinose, galactose, and rhamnose, the molar percentage of arabinose being not less than 70%; the main chain backbone is modified with natural sulfate groups, with the sulfate groups replacing at least one hydroxyl site of the arabinose residues. This invention, when applied to anticoagulation, exhibits good safety and a significant anticoagulant effect.

The invention discloses lotus leaf homogeneous polysaccharide LLP3-1 as well as a separation and purification method and application thereof, and belongs to the technical field of plant polysaccharide. The lotus leaf homogeneous polysaccharide LLP3-1 is composed of rhamnose, arabinose, galactose and galacturonic acid, the molar percentage of monosaccharide is 21.95: 25.33: 21.10: 31.62, the lotus leaf homogeneous polysaccharide LLP3-1 is acidic pectin polysaccharide with RG-I as a main structural domain and with part of side chains, the main chain is mainly composed of galacturonic acid and rhamnose, and the side chains are mainly composed of arabinose and galactose. The lotus leaf homogeneous polysaccharide LLP3-1 has the beneficial effects that the lotus leaf homogeneous polysaccharide LLP3-1 can obviously improve the symptoms of ulcerative colitis; the compound can be widely applied to preparation of medicines for treating ulcerative colitis, medicines for inhibiting proinflammatory factor secretion, antioxidation medicines, medicines for improving intestinal barrier integrity and functions, medicines for promoting short-chain fatty acid generation and medicines for improving intestinal flora disorder of ulcerative colitis.

Intumescent composition comprising an acid donor (A), a carbon donor (B), a binder polymer (C) and a blowing agent (D). The acid donor (A) is selected from the group consisting of ammonium polyphosphate, ammonium dihydrogen phosphate, ethylenediamine phosphate, ammonium pentaborate, guanylurea phosphate, diguanidine phosphate and mixtures thereof. The carbon donor (B) is selected from the group consisting of glucose, arabinose and other monosaccharides, lactose, maltose and other disaccharides, starch, cellulose, dextrin and other polysaccharides, sorbitol, erythritol, pentaerythritol, dipentaerythritol, mannitol and other polyhydric alcohols and mixtures thereof. The blowing agent is provided in the form of microsphere particles comprising a thermoplastic polymer shell encapsulating a propellant, which microsphere particles are expandable microspheres.

This invention provides a Shoumei polysaccharide, its preparation method, and its application, belonging to the field of biomedical technology. The Shoumei polysaccharide is a homogeneous polysaccharide with the repeating sequence →4)-α-D-Gal. p A‑(1→, →4)‑β‑D‑Gal p ‑(1→,→4,6)‑β‑D‑Gal p ‑(1→,→6)‑β‑D‑Gal p -(1→、α-L-Ara f ‑(1→,→5)‑α‑L‑Ara f ‑(1→,→4)‑β‑D‑Glc p -(1→; The molecular weight of the Shoumei polysaccharide is 1100 kDa; The monosaccharide composition of the Shoumei polysaccharide is arabinose, galactose, glucose and galacturonic acid; The molar ratio of arabinose, galactose, glucose and galacturonic acid is 10.9:12.1:4.8:72.2, and the degree of galacturonic acid esterification is 33%. The Shoumei polysaccharide of the present invention can treat and / or prevent depression.

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The application discloses a ginseng polysaccharide, a preparation method and application, and the ginseng polysaccharide is ginseng polysaccharide GP60, which is composed of glucose, galactose and arabinose, and the molar ratio of each monosaccharide is glucose: galactose: arabinose = 77.1: 16.8: 6.1; a new preparation method is adopted; after soaking and twice decocting, different proportions of 95% ethanol are added into the filtrate in batches; the obtained precipitate is added with water to prepare a solution with a relative density of 1.1 g / ml; a certain amount of the solution is taken, a certain proportion of chloroform and n-butanol is added, and a polysaccharide aqueous solution is prepared; the polysaccharide aqueous solution is added with 95% ethanol until the alcohol content reaches 60%; the solution is statically placed for 12 hours; centrifugal separation is carried out; the precipitate is collected; the collected precipitate is sequentially washed with 95% ethanol, anhydrous ethanol, isopropyl alcohol and ether; the solvent is volatilized; and the ginseng polysaccharide GP60 is obtained. The ginseng polysaccharide GP60 is non-toxic, has the effect of preventing light aging, and can be widely applied in the fields of food or health food, anti-light aging skin care products, washing and caring products and anti-light aging medicines.

The invention belongs to the technical field of biological medicine, and particularly relates to wheat bran araboxylan and application thereof. The invention provides wheat bran AX with specific molecular weight and monosaccharide composition. The wheat bran AX comprises the following monosaccharides: 0.20%-0.22% of glucuronic acid, 0.58%-0.62% of galacturonic acid, 0.21%-0.23% of rhamnose, 0.6%-0.7% of glucose, 1.4%-1.5% of galactose, 18%-19.2% of xylose and 21%-23% of arabinose. Compared with commercially available wheat bran AX, inulin, fructo-oligosaccharide and other common dietary fibers, the self-made wheat bran AX has higher diversity of microbial communities in the fermentation process, has a better intestinal flora regulating effect, further has a good relieving effect on the abnormal glucose and lipid metabolism of the body, and can be used for preventing and treating the abnormal glucose and lipid metabolism of the body. Therefore, the traditional Chinese medicine composition has a good regulation effect on various diseases such as non-alcoholic fatty liver and the like caused by abnormal glucose and lipid metabolism of the organism.

The embodiment of the invention discloses citrus peel pectin, citrus peel pectin emulsion and application. The content of the citrus peel pectin is 66-67%, the protein content is 13-14%, and the esterification degree is 72-73%; the sugar comprises the following components in percentage by mole: 58-59% of galacturonic acid, 25-26% of arabinose, 5-6% of galactose, 4-5% of rhamnose, 2-3% of glucose and 2-3% of xylose. The citrus peel pectin provided by the invention has excellent emulsibility; the citrus peel pectin emulsion has excellent emulsion stability, and shows a better encapsulation effect on LBE. The LBE emulsion obtained by encapsulating LBE by using CP has the characteristics of high encapsulation efficiency, low toxicity and high biocompatibility, and the synergistic effect of CP obviously enhances the antioxidant activity of LBE and the activity inhibition rate of tyrosinase, elastase and hyaluronidase, so that the LBE emulsion can be used as a novel anti-aging emulsion formula to be applied to cosmetics, and has a good application prospect. Good application prospects are realized.

The invention relates to the technical field of enzyme engineering, in particular to a xylose reductase XR21 mutant and application thereof. Specifically, the invention provides a novel xylose reductase mutant aiming at the defect of poorer thermal stability of the existing wild type xylose reductase, and particularly provides a xylose reductase mutant which is obtained by performing single or multiple site amino acid mutation on the 19th site, the 48th site, the 79th site, the 208th site and the 300th site of a xylose reductase sequence shown as SEQ ID NO: 2, compared with wild type xylose reductase, the obtained xylose reductase mutant has the advantages that the activity of catalyzing an epimer substrate arabinose into arabitol can be obviously reduced, and meanwhile, the selectivity to xylose is improved, so that the operation process of downstream separation and purification is simplified, and the biological catalysis requirement of green industry is met.

The application discloses Ixeris sonchifolia Hance polysaccharides, a preparation method and application thereof, belongs to the technical field of plant polysaccharides, and provides polysaccharides ISP-1a and ISP-2a, wherein the ISP-1a has a molecular weight of 2086 Da and is composed of seven monosaccharides of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose; the ISP-2a has a molecular weight of 1781 Da and is composed of five monosaccharides of mannose, rhamnose, galacturonic acid, xylose and arabinose. The whole grass of Ixeris sonchifolia Hance is subjected to hot water extraction after ethanol degreasing, neutral enzyme + Sevage method is used to remove protein, macroporous resin is used for decolorization, column chromatography elution is carried out, purification is carried out, and two kinds of uniform polysaccharides are obtained after freeze-drying. The polysaccharides ISP-1a and ISP-2a have good antioxidant and lipid-lowering effects, and can be applied to the preparation of food, health products or medicines with antioxidant and lipid-lowering functions.

The present application relates to the technical field of Chinese medicine beverage preparation, in particular to a Chinese medicine fermentation sugar control and stomach nourishing beverage and a preparation method thereof, which is prepared from the following raw material components by weight: Ganoderma lucidum 1-5 parts, Momordica grosvenori 1-5 parts, Cinnamomum cassia 1-3 parts, Foeniculum vulgare 5-10 parts, Herba Massa Medicata Fermentata 15-25 parts, Poria cocos 10-25 parts, Astragalus membranaceus 15-25 parts, milk 600-1000 parts, pure water 200-800 parts, Momordica grosvenori glycoside powder 15-25 parts, L-arabinose 10-15 parts, xylitol 20-30 parts, glucose 10-20 parts, and corn flour 30-50 parts. The technology uses bidirectional fermentation technology and innovatively combines traditional Chinese medicine processing technology and modern fermentation technology. The technical scheme successfully integrates traditional wisdom and modern technology, and finally obtains a beverage with the dual health functions of sugar control and stomach nourishing.

The application belongs to the technical field of natural products, and discloses purified fermentation dendrobium polysaccharide and application thereof in skin barrier repair. The application provides a fermentation dendrobium polysaccharide DOP-1A, which comprises mannose, arabinose, gluconic acid, glucose, galactose and fucose; and a fermentation dendrobium polysaccharide DOP-2A, which comprises mannose and glucose. The fermentation dendrobium polysaccharide with high purity and uniform structure is obtained by using a fermentation dendrobium powder extraction solution, alcohol precipitation and column chromatography purification. Research proves that the fermentation dendrobium polysaccharide obtained by separation and purification has the effects of skin moisturizing and skin barrier repair, and also has anti-inflammatory activity. The extraction and separation method provided by the application can efficiently extract and purify polysaccharide from fermentation dendrobium, is suitable for the fields of skin barrier repair and anti-inflammatory, and has good application prospect and value.