430 results about "Arabinose" patented technology

The invention discloses an attenuated salmonella typhimurium EN-VNP (Enhanced Natriuretic Peptide) strain as well as a preparation method and application of the attenuated salmonella typhimurium EN-VNP strain. Comprising the following steps: on the basis of a salmonella VNP20009 strain, flagellin genes fliC and fljB, a phosphoethanolamine transferase gene eptA, a 4-amino-4-deoxy-L-arabinose transferase gene arnT and phosphotransferase yeiU are sequentially knocked out or silenced, and a strain is formed after a phosphotransferase gene lpxE is knocked in or overexpressed, so that the attenuated salmonella typhimurium EN-VNP strain is prepared. According to the attenuated salmonella typhimurium EN-VNP with low toxicity and the T cell adjuvant effect and an EN-OMV antigen delivery system prepared from the attenuated salmonella typhimurium EN-VNP, an improved strain does not contain flagellin, an LPS molecular structure is changed, the attenuated salmonella typhimurium EN-VNP has the low toxicity and the T cell adjuvant effect, and normal multiplication culture can be achieved.

The invention belongs to the field of new application of medicines, and particularly relates to gastrodia elata polysaccharide and application thereof. The gastrodia elata polysaccharide component GEP-2 is separated from crude gastrodia elata polysaccharide, structural characterization detection is conducted on the GEP-2, the result shows that the gastrodia elata polysaccharide GEP-2 is prepared from, by mass, 89.08% of glucose, 2.40% of galactose, 7.12% of galacturonic acid, 0.86% of arabinose and 0.54% of rhamnose, and the animal experiment result shows that the GEP-2 relieves the symptoms of mouse colitis, relieves weight loss and shortens the colon length; the traditional Chinese medicine composition has the advantages of reducing serum TNF-alpha, IL-1beta and IL-6 proinflammatory cytokine levels, improving the intestinal barrier function, reducing inflammatory cell infiltration, regulating the intestinal flora composition structure of mice and relieving ulcerative colitis.

This invention provides an in vitro cell-free protein synthesis system, its reagent kit, and its applications, belonging to the field of protein synthesis technology. This invention improves an in vitro cell-free protein synthesis system based on Kluyveromyces lactis by adding exogenous L-arabinose, significantly enhancing the system's protein synthesis capacity. Furthermore, it provides a more efficient and higher-throughput in vitro protein synthesis reagent kit and a method for synthesizing exogenous proteins. The improvements provided by this invention can be achieved without molecular modification, are simple and convenient to operate, and save costs.

The invention discloses application of a ganoderma lucidum strain SS30 in preparation of a product for treating cancers, and belongs to the technical field of biology. The invention provides ganoderma lucidum strain SS30 mycelium polysaccharide. The ganoderma lucidum strain SS30 mycelium polysaccharide is prepared from glucose, galactose, mannose, fucose, xylose, arabinose, rhamnose, glucuronic acid, glucosamine, ribose and lacturonic acid. The polysaccharide has a remarkable effect of inhibiting transplantable pancreatic cancer and primary colorectal cancer, can achieve the effect of treating cancer by improving the immune function and reducing the oxidation product generation level, provides a new material for development and utilization of lucid ganoderma and products thereof, and has a wide application prospect. And a research basis is provided for research and development of cancer-related drugs and research of a cancer treatment mechanism.

Proposed are recombinant entomopathogenic bacteria transformed using promoter replacement technique, a preparation method, and uses thereof for producing pesticides and controlling pests. Provided are recombinant entomopathogenic bacteria in which the promoter of the GXP synthase gene (gxpS gene) is replaced in the entomopathogenic bacteria with an arabinose inducible promoter using a promoter replacement technique. The recombinant entomopathogenic bacteria prepared in the present disclosure can produce large quantities of GXPs, which acts to suppress immunity of pests, by controlling the expression of the GXP synthetase gene (gxpS gene) through an addition of an inducer. The recombinant entomopathogenic bacteria prepared in the present disclosure can also effectively control pests through a response that suppresses the immunity of pests.

The utility model belongs to the technical field of sugar alcohol preparation, and relates to a system for co-producing L-arabinose and caramel pigment by using xylose chromatography raffinate, which comprises a raw material tank, a nanofiltration membrane separator, a chromatography separation device, an evaporation concentration tank, a crystallization tank, a centrifugal separator and a dryer which are sequentially communicated by pipelines, and the browning reaction assembly is used for mixing the raffinate and the centrifugal mother liquor and then carrying out browning reaction treatment to prepare caramel pigment. The chromatographic separation device is used for separating an extracting solution and raffinate from the flowing nanofiltration permeate, the evaporation concentration tank and the crystallizing tank are used for concentrating and crystallizing the flowing extracting solution into massecuite, the centrifugal separator is used for separating the flowing massecuite into moist sugar and centrifugal mother liquor, and the dryer is used for drying the flowing moist sugar into L-arabinose crystals. According to the utility model, raffinate and centrifugal mother liquor are mixed to prepare a caramel pigment liquid product, so that a caramel pigment additional product is obtained while the utilization rate of saccharide resources is improved.

The application provides a Huangdache oligosaccharide with GLP-1 agonist activity and application thereof, and belongs to the technical field of medicines. 3 The oligosaccharide component ELYP-3 has a molecular weight of 3.2*10 3 Da, and the molar ratio of monosaccharides is rhamnose:galacturonic acid:glucose:galactose:arabinose = 1:32.42:45.37:9.11:6.54. The Huangdache oligosaccharide fragment obtained by glycosidase degradation has significant hypoglycemic and weight loss activities, the preparation process is simple, specific and green, and provides scientific basis and theoretical support for the development of natural GLP-1 agonists. Meanwhile, the functional oligosaccharide provides a wide application prospect for the development of medicines with blood glucose regulation.

The invention belongs to the technical field of biology, and particularly relates to a method for producing xylitol through two-step enzyme catalysis of hemicellulose hydrolysate, and the method comprises the following steps: taking hemicellulose hydrolysate as a raw material, adding arabinose dehydrogenase, NADH oxidase and coenzyme NADH, and carrying out first-step fermentation until no L-arabinose residue exists in fermentation liquor to obtain fermentation reaction liquor I; and adding xylose reductase and glucose dehydrogenase into the fermentation reaction liquid I, and carrying out second-step fermentation to obtain a fermentation product containing xylitol. According to the two-step enzyme catalysis method provided by the invention, xylitol is prepared, and high-purity xylose does not need to be used as a raw material; according to the present invention, the cheap hemicellulose hydrolysate can be directly adopted as the raw material, the xylose conversion rate and the xylitol yield can exceed 99%, the impurity arabitol content in the product is low, the other impurity saccharic acid is easy to separate, and the problems of complex process, large separation difficulty and high cost in the xylitol refining process are successfully solved.

The present invention discloses an anti-non-small cell lung cancer active birch inonotus polysaccharide IOP-1 and its application, belonging to the field of biomedicine. The present invention adopts a method of boiling water extraction and ethanol precipitation of birch inonotus polysaccharide, and combines DEAE cellulose column purification technology, and utilizes the principle that macromolecular polysaccharides are insoluble in organic solvents to successfully achieve the separation of birch inonotus polysaccharide and obtain a birch inonotus polysaccharide IOP-1 with anti-non-small cell lung cancer activity, wherein the monosaccharide composition of the birch inonotus polysaccharide IOP-1 includes fucose, rhamnose, arabinose, galactose, glucose, xylose and mannose with a molar mass ratio of 2.41:0.58:1.03:2.17:82.20:4.33:7.29. Its significant effect against non-small cell lung cancer was verified by in vitro and in vivo experiments, providing a new treatment method for the clinical treatment of non-small cell lung cancer.

The invention discloses a preparation method of avocado soup rich in eucommia ulmoides seeds, which comprises the following steps: modifying chickpea protein isolate (CPI) through dynamic high-pressure microjet (DHPM), assisting grafting modification of CPI and arabinose (AB) by DHPM, preparing nano-emulsion rich in eucommia ulmoides seed oil by using the modified chickpea protein isolate (CPI) as an emulsifier, and adding the nano-emulsion into the avocado soup to improve the fat-soluble nutrient content of the avocado soup.

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention discloses application of uncaria acidic polysaccharide URP-2 in preparation of an anti-esophageal cancer drug, and belongs to the technical field of antitumor of medicinal plant polysaccharide. According to the method, a method for extracting the uncaria total polysaccharide by boiling water and precipitating the uncaria total polysaccharide by ethanol is adopted, and a DEAE DE-52 cellulose column and sephadex column purification technology is combined, so that the separation of the uncaria acidic polysaccharide component is realized, and the uncaria acidic polysaccharide URP-2 is obtained. The uncaria acidic polysaccharide URP-2 comprises the following components in mole percent: 14.11% of arabinose, 12.64% of rhamnose, 6.48% of galactose, 5.00% of glucose and 61.77% of galacturonic acid. Experiments show that the uncaria acidic polysaccharide URP-2 has remarkable anti-esophageal cancer activity, so that a potential experimental basis and a new strategy are provided for applying the uncaria acidic polysaccharide URP-2 to clinical treatment of esophageal cancer.

The invention discloses a preparation method and application of roasted barley and malt polysaccharide with an anti-cancer effect. The roasted barley malt polysaccharide is a neutral heteropolysaccharide which mainly takes-> 3)-beta-D-Glcp-(1-> and->-beta-D-Glcp-(1->) as main chains, branches exist at O-3 / O-4 positions, and the branch chains are mainly composed of-> 4)-beta-D-Xylp-(1->,-> 3, 4)-beta-D-Xylp-(1-> and alpha-D-Glcp-(1->. The monosaccharide composition comprises arabinose, glucose and xylose in a molar mass ratio of 1.86: 33.43: 1.00, and the molecular weight is 23.81 kDa. In-vitro activity experiments prove that the roasted barley malt polysaccharide disclosed by the invention has a remarkable inhibition effect on proliferation of liver cancer cells, lung cancer cells, breast cancer cells and colorectal cancer cells, particularly on the liver cancer cells, and shows outstanding anti-tumor activity, so that the roasted barley malt polysaccharide is expected to be developed and applied in the fields of foods, health-care products or medicines.

The invention relates to the technical field of plant extraction, in particular to a multi-product preparation method based on an arabinoxylan-lignin compound. The method comprises the following steps: treating a lignocellulose raw material by adopting a weak base extracting solution and a strong base extracting solution, and separating an obtained strong base extracting and dealkalizing suspension into an arabinoxylan-lignin compound dispersion liquid and arabinoxylan; performing enzymolysis on the dispersion liquid, and filtering enzymatic hydrolysate to obtain a precipitate which is lignin and a filtrate which is xylooligosaccharide liquid; and separating and purifying the xylooligosaccharide liquid to obtain xylooligosaccharide, xylose and arabinose. According to the method, on the basis of a weak base-strong base two-step extraction process, obtained arabinoxylan-lignin compound dispersion liquid is subjected to enzymolysis, so that lignin, xylooligosaccharide, xylose and arabinose can be continuously prepared, and the reutilization of the arabinoxylan-lignin compound is realized.

The invention discloses a composition for regulating body weight through satiety-sugar control double channels. The composition is prepared from the following components in parts by weight: 5-25 parts of cinnamon extract, 1-20 parts of ganoderma lucidum EGCG composite powder, 10-40 parts of L-arabinose, 5-40 parts of composite fruit and vegetable fiber powder, 5-25 parts of fructus cannabis protein peptide, 1-20 parts of apple polyphenol, 1-20 parts of white kidney bean dietary fiber powder and 1-20 parts of agaricus bisporus powder. The composition is characterized in that digestion and absorption of carbohydrate are inhibited, the swelling ratio larger than or equal to 35 times is achieved through a composite fiber system, gastric emptying is remarkably prolonged, satiety hormones such as GLP-1 are adjusted, and the composition can be used as a non-alternative weight management product and is good in safety.

The invention discloses a polysaccharide compound of Alsophila spinulosa and an extraction method and application thereof, wherein the polysaccharide compound is composed of rhamnose, arabinose, galactose and glucuronic acid. The polysaccharide compound of the invention is derived from mature leaves of Alsophila spinulosa, and is extracted by water extraction and alcohol precipitation, crude polysaccharide impurity removal, DEAE seplife FF ion column separation, and Sephacryl S‑400HR gel column purification. The method is simple and fast to operate, and water extraction and anhydrous ethanol precipitation are mainly used in the extraction and separation process. The process method is environmentally friendly, and the purity of the compound separated by the method is relatively high, all greater than 92%. The extracted polysaccharide compound has been found to have a significant hypoglycemic effect through research, and can be used as a raw material for new drug development and pharmacological activity research, and can also be used for food additives or health care product development.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention discloses allium fistulosum and bolete polysaccharide as well as a preparation method and application thereof. The allium fistulosum and bolete polysaccharide is a glycopolymer composed of arabinose, xylose, mannose, glucose and galactose. The allium fistulosum bolete polysaccharide disclosed by the invention has strong antioxidant capacity, has a stimulation and activation effect on macrophage immune response, has an inhibition effect on alpha-glucosidase, has the effects of resisting oxidation, enhancing immunity and reducing blood sugar, and can be applied to development of health-care foods and functional foods.

The invention relates to the technical field of plant polysaccharides, and particularly discloses a red-knees herb polysaccharide and a preparation method and application thereof, the peak top molecular weight of the red-knees herb polysaccharide is 33.9 KDa, the weight-average molecular weight of the red-knees herb polysaccharide is 42.8 KDa, the number-average molecular weight of the red-knees herb polysaccharide is 10.0 KDa, and the red-knees herb polysaccharide is mainly composed of galacturonic acid, galactose, arabinose, rhamnose and glucuronic acid. According to the preparation method of the red-knees herb polysaccharide, by optimizing process parameters and controlling the reasonable solid-liquid ratio, heating temperature and heating time, the dissolution rate of the polysaccharide is increased, the dissolution rate of impurities is reduced, and the yield of the red-knees herb polysaccharide is increased. The red-knees herb polysaccharide is used as a raw material, ethanol is used for alcohol precipitation, polysaccharide components are precipitated, impurities are preliminarily removed, then macroporous resin is used for purification, the recovery rate of polysaccharide and the removal rate of impurities such as pigment and protein are improved by screening optimization and pretreatment of the macroporous resin, and the total sugar content of the purified red-knees herb polysaccharide is 80% or above. Use of toxic chemical reagents is avoided, and the preparation method is safe and efficient.

The invention provides application of non-starch Chinese chestnut polysaccharide, the non-starch Chinese chestnut polysaccharide comprises polysaccharide CNP-1, polysaccharide CNP-2 and polysaccharide CNP-1, the weight-average molecular weight of the polysaccharide CNP-1 is 4.82 * 10 < 3 > kDa, the polysaccharide CNP-1 comprises glucose, galactose, arabinose, rhamnose, fructose, fucose, xylose and glucosamine hydrochloride, the molar ratio of the glucose to the galactose to the arabinose to the rhamnose to the fructose to the fucose to the xylose to the glucosamine hydrochloride is 0.626: 0.121: 0.084: 0.071: 0.061: 0.023: 0.011: 0.003; the polysaccharide CNP-2b has the weight-average molecular weight of 1.25 * 10 < 4 > kDa and comprises glucose, galactose, arabinose, rhamnose, fructose, fucose, xylose and glucosamine hydrochloride according to the molar ratio of (0.505): (0.161): (0.148): (0.077): (0.052): (0.025): (0.027): 0.003; the non-starch polysaccharide can regulate the intestinal flora abundance caused by colitis, improve the intestinal flora structure, maintain the intestinal steady state, relieve the injury degree of colon and ensure the body health.

The present invention discloses roxburghii polysaccharide and its preparation method and application. Wherein roxburghii crude polysaccharide is purified to obtain two polysaccharides RP1 and RP3, RP1 monosaccharide types include galacturonic acid, galactose, rhamnose and arabinose, and its molar ratio is 1.00:3.45:1.88:1.82, RP3 monosaccharide types include galactose, rhamnose and arabinose, galacturonic acid, galactose, rhamnose and arabinose, and its molar ratio is 1.00:0.37:0.37:0.25. It has been confirmed by in vitro experiments that the above-mentioned roxburghii polysaccharide has the ability to significantly inhibit the proliferation of MCF-7 breast cancer cells, as well as the effect of inducing apoptosis of MCF-7 breast cancer cells, and can be developed to prepare breast cancer therapeutic drugs; At the same time, the above-mentioned roxburghii polysaccharide has no effect on normal L929 cells. Therefore, the above-mentioned roxburghii polysaccharide has broad application prospects and has good social and economic benefits.

The invention provides an antibiotic-free host-plasmid TA maintenance system and application thereof, and relates to the technical field of gene engineering. According to the invention, a toxin gene CcdB is accurately integrated to a host escherichia coli chromosome fhuA site through a gene editing technology, and accurate control of toxin expression is realized by adopting an inducible promoter. The antitoxin gene is simplified to a plasmid skeleton, and the toxin inhibition function is maintained through constitutive expression. According to the method, plasmid-free cells are eliminated through arabinose-induced toxin expression, so that stable maintenance of plasmids is realized (the loss rate is lt; and the method completely avoids the use of antibiotics, meets the biological safety requirements, is suitable for large-scale production of vaccines, recombinant proteins and other biological products, and has the advantages of high plasmid yield, low environmental leakage risk, process compatibility and the like.

The invention discloses low-molecular-weight enteromorpha polysaccharide with antioxidant activity and a preparation method of the low-molecular-weight enteromorpha polysaccharide, and belongs to the technical field of polysaccharide preparation. The preparation method comprises the following steps: taking an enteromorpha crude polysaccharide solution, adding H2O2 to enable the concentration of the enteromorpha crude polysaccharide solution to be 0.04-0.08 M, and degrading for 90-120 minutes under ultraviolet rays to obtain the low-molecular-weight enteromorpha polysaccharide, the low-molecular-weight enteromorpha polysaccharide is composed of monosaccharides of rhamnose, glucose, galactose, xylose, mannose and arabinose according to the ratio of 1.08: 1.00: 0.83: 0.58: 0.69: 0.06, the scavenging capacity and the iron reducing capacity of DPPH, ABTS and hydroxyl free radicals are improved, and the low-molecular-weight enteromorpha polysaccharide is obtained. The compound has excellent antioxidant activity and can be used as or used for preparing an antioxidant active preparation. The preparation method disclosed by the invention is efficient and stable, can be used for preparing the low-molecular-weight enteromorpha polysaccharide with lighter color, lower molecular weight and higher antioxidant activity, and has higher potential application value.

This application relates to a class of seven-membered ring mimetic sugar compounds, their preparation methods, and applications. The compounds have the structure shown in Formula I. #imgabs0# This application synthesizes the compounds shown in Formula I and their pharmaceutically acceptable salts using D-arabinose as a raw material. The compounds and their pharmaceutically acceptable salts exhibit strong glycosidase inhibitory activity and are used to treat diseases caused by coronaviruses, influenza viruses, and the like.

The invention discloses arabinose glycosyl transferase PnUGT73B4 and application thereof to preparation of panax japonicus saponin IV, the amino acid sequence of the arabinose glycosyl transferase is shown as SEQ ID NO: 1, under the catalysis of the arabinose glycosyl transferase PnUGT73B4, UDP-arabinose is added to a glucuronic acid group on the C3 site of panax japonicus saponin IVa, and the panax japonicus saponin IV is generated; according to the method, the requirement for raw material plants can be reduced, agricultural land is saved, the product is single, preparation of the panax japonicus saponin IV is facilitated, the biosynthesis route of oleanane type triterpenoid saponin is analyzed, and a breakthrough solution is provided for biosynthesis of the panax japonicus saponin IV.

The invention discloses purified barley seedling polysaccharide as well as a preparation method and application thereof. The polysaccharide is prepared from six monosaccharides, namely rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 2.88: 3.39: 1.51: 1: 0.76: 3.2. The relative molecular weight is 254.09 kDa. A fermentation method is combined with an innovative purification process, so that the barley seedling polysaccharide monomer with high purity and uniform structure is obtained for the first time. Through a multi-dimensional characterization technology, fine structure characteristics such as monosaccharide composition, glucosidic bond connection mode and branching degree are systematically analyzed, and a reliable material basis is provided for establishing structure-function accurate association. The purified barley seedling polysaccharide has a remarkable effect of promoting intestinal peristalsis, not only lays a scientific foundation for deep processing of barley seedlings and research and development of related functional products, but also provides a new theoretical support for relieving intestinal peristalsis disorder, and has a wide application prospect.

The invention discloses a composite plant polypeptide composition with the effects of repairing pancreas and reducing blood sugar. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of ginsenoside, 5 to 30 parts of radix astragali seu hedysari extract, 3 to 20 parts of radix rehmanniae polysaccharide, 2 to 15 parts of radix trichosanthis alkaloid, 5 to 25 parts of rhizoma dioscoreae polysaccharide, 5 to 30 parts of bitter gourd polypeptide, 3 to 20 parts of folium mori alkaloid, 8 to 40 parts of rhizoma polygonati polysaccharide, 5 to 25 parts of fructus hippophae flavone, 10 to 35 parts of wheat germ peptide, 2 to 15 parts of coptis berberine, 10 to 45 parts of corn stigma polysaccharide and 3 to 20 parts of saussurea involucrata polyphenol. 1-10 parts of sophora flavescens total alkaloids, 5-25 parts of a dendrobium extract, 5-30 parts of a radix puerariae extract, 8-35 parts of L-arabinose and 10-40 parts of small molecule peptides; 20 to 100 parts of collagen peptide and 15 to 80 parts of albumin peptide; 5-50 parts of microcrystalline cellulose, 3-30 parts of fructo-oligosaccharide and 0.5-5 parts of magnesium stearate; the limitation of single sugar control or simple component mixing in the prior art is broken through, a synergistic system of plant active components and functional polypeptides is constructed, and the purity and retention rate of the plant active components are improved by optimizing the extraction process.

The invention discloses neutral homogeneous lycium barbarum polysaccharide as well as a preparation method and application thereof. Through systematic structure characterization, it is clarified for the first time that the polysaccharide NLBPE1 is atypical arabinogalactan with 1, 5-alpha-L-arabinose as a bridging unit, and the polysaccharide NLBPE1 has a uniform molecular weight and a definite branched chain topological structure. The NLBPE1 serving as an adjuvant and an antigen are jointly entrapped in various delivery systems (such as PLGA nanoparticles, lipidosome, hydrogel and the like) to form the vaccine composition, so that the immunogenicity of the vaccine can be remarkably improved, and the antigen-specific T cell response can be enhanced. The invention provides an application scheme of the polysaccharide as a vaccine adjuvant in tumor immunotherapy and tumor prevention, and particularly shows a remarkable synergistic anticancer effect when the polysaccharide is combined with an immune checkpoint inhibitor for use. The polysaccharide has a clear structure, good biocompatibility and remarkable immunological enhancement activity, and has a wide application prospect of tumor vaccine adjuvants.

The invention provides flash-soluble direct-drinking fungus powder and a preparation method thereof. The flash-soluble direct-drinking bacterial powder comprises the following components in parts by weight: 65-75 parts of erythritol, 10-16 parts of stachyose, 2-5 parts of L-arabinose, 1-3 parts of mono-and diglycerides of fatty acids, 2-5 parts of resistant dextrin and 2-10 parts of probiotics or metabiotics. By adopting the stachyose and the L-arabinose in parts by weight, the sweetness of the flash-soluble direct-drinking fungus powder is effectively reduced; on the premise of being matched with other components, the oligosaccharide shows the performance completely different from that of conventional oligosaccharide, particularly erythritol, stachyose and L-arabinose can be fully dispersed under the action of mono-and diglycerides of fatty acids, so that the effect advantages of the oligosaccharide can be better played, and the flash-soluble direct-drinking fungus powder can keep the flash-soluble characteristic and can be used for preparing the instant instant fungus powder. The oral liquid is quick to melt in the oral cavity, easy to swallow, strong in impact feeling and cool feeling, weak in mouth choking degree and mouth stickiness, weak in granularity, and weak in oral cavity covering feeling and oral cavity drying feeling of whole residues after swallowing.

Hippophae rhamnoides peel polysaccharides, and a preparation method therefor and a use thereof. The hippophae rhamnoides peel polysaccharides comprise the following monosaccharides: rhamnose, mannose, galactose, glucose, galacturonic acid, and arabinose. The preparation method comprises: degreasing, extracting, concentrating and performing alcohol precipitation to obtain water-soluble crude polysaccharides; and then using a non-polar macroporous adsorption resin to perform purification to obtain hippophae rhamnoides peel polysaccharides. The yield and content of the polysaccharides are high. By using the non-polar microporous adsorption resin to replace a relatively costly DEAE-cellulose and Sephadex gel, active polysaccharides in the Hippophae rhamnoides peel are enriched, the yield and purity of the polysaccharides are also greatly improved, and the polysaccharides have low production costs and are used for industrial batch production. The hippophae rhamnoides peel polysaccharides have significant effects of resisting ulcerative colitis, and alleviating constipation, indigestion, and intestinal flora disorder.