39 results about "Allose" patented technology

An antiseptic cleansing composition comprising an antimicrobial agent, an effective amount of an alkylpolysaccharide surfactant, at least one alkyl alcohol and at least one aryl alcohol. Suitable surfactant alkylpolysaccharides may contain one or more sugar units selected from the group consisting of maltose, arabinose, xylose, mannose, galactose, gulose, idose, talose, allose, altrose, sucrose, fructose, sorbose, levulose, lactose, allulose, tagatose, alloheptulose, sedoheptulose, glucoheptulose, mannoheptulose, guloheptulose, idoheptulose, galactoheptulose, taloheptulose and derivatives thereof. Suitable antimicrobial agents include chlorhexidine, chlorhexidine salt, chlorophenol derivative, octenidindihydrochloride (CH3—(CH2)7—NHON—(CH2)10—NO—NH(CH2)7—CH2 or any other salt thereof, and quaternary ammonium compounds.

It has been discovered that fracturing fluid breaker mechanisms are improved by the inclusion of a catalyzed polyol alone that directly degrades the polysaccharide backbone, and optionally additionally by removing the crosslinking ion, if present. That is, viscosity reduction (breaking) occurs by breaking down the chemical bonds within the backbone directly. The gel does not have to be crosslinked for the method of the invention to be successful, although it may be crosslinked. In one non-limiting embodiment, the polyol has at least two hydroxyl groups on adjacent carbon atoms. In another embodiment, the polyols are simple sugars and sugar alcohols, and may include mannitol, sorbitol, glucose, fructose, galactose, mannose, lactose, maltose, allose, etc. and mixtures thereof. The catalyzing metal ion may employ a metal selected from Groups VIB, VIIB, VIII, IB, and IIB of the Periodic Table (previous IUPAC American Group notation).

A method of utilizing the physiological activity of a rare saccharide, wherein physiological-activity sensitive cells are treated with the rare saccharide to modify the function of the cells. A composition containing, as an active ingredient, a rare saccharide which is introduced into physiological-activity sensitive cells and has an effect of modifying the function of the cells. The cells are human cells. The composition is a functional food, a drug, or a cosmetic. The rare saccharide is a rare saccharide belonging to aldose and / or ketose. The aldose is D-allose, and the cells are selected from the group consisting of cancer-cell proliferation inhibitory activity sensitive cells and active-oxygen production inhibitory activity sensitive cells. The ketose is D-psicose, and the cells are selected from the group consisting of chemokine secretion inhibitory activity sensitive cells, microglia migration inhibitory activity sensitive cells, and hypoglycemic activity sensitive cells.

[OBJECT] To provide an agricultural chemical and the like with the use of an effect of inducing systemic acquired resistance in a plant. To provide a growth inhibitor of not only a plant pathogenic bacterium but also a harmful microorganism.[MEANS FOR RESOLUTION] Utilization of a rare sugar for inducing systemic acquired resistance in a plant or inhibiting the growth of a microorganism. Utilization thereof as an agricultural chemical with the use of the effect of inducing systemic acquired resistance in a plant, a plant disease inhibitor, an inducer of a plant growth regulatory factor (i.e., an inducer of plant hormone-like actions consisting of disease resistance, insect resistance, fruit maturation, breaking of dormancy, regulation of germination, drying resistance, and other than this, resistance to environmental stresses such as low temperature resistance, high temperature resistance, salt resistance and heavy metal resistance and promotion of flowering) and a microorganism growth inhibitor. The rare sugar is an aldose (D-allose, D-altrose or L-galactose) or a ketose (D-psicose or a mixture of D-psicose and D-fructose).

It has been discovered that fracturing fluid breaker mechanisms are improved by the inclusion of a polyol alone that directly degrades the polysaccharide backbone, and optionally additionally by removing the crosslinking ion, if present. That is, viscosity reduction (breaking) occurs by breaking down the chemical bonds within the backbone directly, rather than by merely removing the crosslinking ion, if present. The gel does not have to be crosslinked for the method of the invention to be successful, although it may be crosslinked. In one non-limiting embodiment, the polyol has at least two hydroxyl groups on adjacent carbon atoms. In another embodiment, the polyols are monosaccharides such as glycerols and sugar alcohols, and may include mannitol, sorbitol, glucose, fructose, galactose, mannose, lactose, maltose, allose, etc. and mixtures thereof.

[Problem] To provide a preservative solution for cells, tissues and organs using a rare sugar and a preservation method using the solution.[Means for Resolution] A preservative solution for cryopreservation of animal or human organs and animal or plant tissues or cells, the solution containing a rare sugar D-allose. A preservative solution for cryopreservation of the human kidney, cells derived from animals or humans, or human or animal sperm and / or ovum and / or fermented ovum in which the cryopreservation is conducted at from −5 to 20° C. A preservative solution for cryopreservation of cells derived from animals or humans, or human or animal sperm and / or ovum and / or fermented ovum in which the cryopreservation is conducted at a temperature at which to start freezing to −196° C. A method for cryopreservation of an animal or human organ and an animal or plant tissue or cell at a low temperature, which comprises (a) refluxing or immersing a preservative solution for cryopreservation containing a rare sugar D-allose and the organ or the tissue or mixing the cell therewith, and (b) cooling the organ, the tissue or the cell to a low temperature of from −5 to 20° C.

[PROBLEMS] To find out a specific rare sugar having effects of inhibiting the proliferation of vascular endothelial cells and lumen formation and utilize these effects. To provide this rare sugar as a preventive / remedy for diseases with angiogenesis, a cosmetic or a functional food.[MEANS FOR SOLVING PROBLEMS] A method of controlling the proliferation of vascular endothelial cells characterized by utilizing the vascular endothelial cell proliferation-controlling effect of D-mannose, D-allose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, L-sorbose, 2-deoxy-D-ribose and / or 2-deoxy-L-ribose. A method of inhibiting lumen formation of vascular endothelial cells characterized by utilizing the vascular endothelial cell lumen formation-inhibiting effect of D-allose, D-altrose, D-gulose, D-talose, L-allose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, D-ribose, L-ribose, 2-deoxy-D-ribose and / or 2-deoxy-L-ribose.

The invention relates to the medicine technology field, in particular relates to a new application of cardiac glycoside chemical compound having general formula (I) in preparing oncotherapy drug, wherein, R equals to 2-O-methyl-Beta-D-mycose, 6-deoxidation-Beta-D-gulose or 6-deoxidation-2-O-methyl-Beta-D-allose. The cardiac glycoside chemical compound can be obtained through a separation from upas in various conventional separation methods or be obtained through a synthesis or semisynthesis method.

The invention relates to gelidium pacificum okam polysaccharide as well as a preparation method and application thereof, and belongs to the field of natural polymers. The invention provides a method for extracting and separating polysaccharide from gelidium pacificum okam, as well as a chemical structure and application of the polysaccharide. The average relative molecular weight is determined byutilizing GPC and the monosaccharide composition is analyzed by using GC-MS; the average relative molecular weight of the gelidium pacificum okam polysaccharide A-11 disclosed by the invention is found to be 28807Da and the gelidium pacificum okam polysaccharide A-11 is mainly prepared from xylose and galactose; in addition, trace amounts of rhamnose, arabinose, fucose, allose, fructose, glucose and mannose are found. Meanwhile, the gelidium pacificum okam polysaccharide A-11 disclosed by the invention has the advantages that the cytotoxicity of LPS-induced THP-1 is significantly inhibited, sothat the cells are prevented from being damaged; besides, the expression of signal factors MyD88 and TRAF-6 in an immune signaling pathway can be regulated so as to enhance human immunity.

A chewing gum base comprises food acceptable substituted polysaccharides wherein substituents on the saccharide units in the polysaccharides produce a degree of substitution of at least I.0. The polysaccharides may have branches with an average length of 1 to 15 saccharide units per branch. The polysaccharides may be linked saccharide units such as allose, altrose, mannose, gulose, idose, galactose, 3,6 anhydro galactose, glucuronic acid, mannuronic acid, galacturonic acid, aldobiouronic acid, fucose, rhamnose, arabinose, xylose, talose, acyl substituted glucose, fructose, lactose and combinations thereof.

A method of utilizing the physiological activity of a rare saccharide, wherein physiological-activity sensitive cells are treated with the rare saccharide to modify the function of the cells. A composition containing, as an active ingredient, a rare saccharide which is introduced into physiological-activity sensitive cells and has an effect of modifying the function of the cells. The cells are human cells. The composition is a functional food, a drug, or a cosmetic. The rare saccharide is a rare saccharide belonging to aldose and / or ketose. The aldose is D-allose, and the cells are selected from the group consisting of cancer-cell proliferation inhibitory activity sensitive cells and active-oxygen production inhibitory activity sensitive cells. The ketose is D-psicose, and the cells are selected from the group consisting of chemokine secretion inhibitory activity sensitive cells, microglia migration inhibitory activity sensitive cells, and hypoglycemic activity sensitive cells.

The present invention relates to a recombinant strain for producing an allose from a fructose, a composition for producing an allose which produces an allose from a fructose-containing raw material comprising the strain, and a method for preparing an allose using the same.

The invention discloses a method for preparing D-allose by reducing ketose by a catalytic hydrogenation process, which comprises the following steps: 1) subjecting the compound of formula (II) to reduction reaction in the presence of a transitional metal catalyst and hydrogen to obtain a compound of a formula III, wherein R1 and R2 are dihydroxyl protective groups; and 2) removing the R1 and R2 protective groups from the compound of the formula III to obtain D-allose. The method has the advantages of high stereoselectivity, high cleanness, convenience for post treatment and the like and is a preparation method suitable for industrial production.

To provide an agricultural chemical and the like with the use of an effect of inducing systemic acquired resistance in a plant. To provide a growth inhibitor of not only a plant pathogenic bacterium but also a harmful microorganism.Utilization of a rare sugar for inducing systemic acquired resistance in a plant or inhibiting the growth of a microorganism. Utilization thereof as an agricultural chemical with the use of the effect of inducing systemic acquired resistance in a plant, a plant disease inhibitor, an inducer of a plant growth regulatory factor (i.e., an inducer of plant hormone-like actions consisting of disease resistance, insect resistance, fruit maturation, breaking of dormancy, regulation of germination, drying resistance, and other than this, resistance to environmental stresses such as low temperature resistance, high temperature resistance, salt resistance and heavy metal resistance and promotion of flowering) and a microorganism growth inhibitor. The rare sugar is an aldose (D-allose, D-altrose or L-galactose) or a ketose (D-psicose or a mixture of D-psicose and D-fructose).

The invention discloses a method for biosynthesizing D-allose by using D-glucose, belongs to the field of escherichia coli metabolic engineering, and aims to synthesize D-allose by using organisms. Asthe content of D-allose in nature is extremely low and the extraction rate is low, the synthesis of D-arabinose in engineering escherichia coli is a content worthy of research. Three exogenous genesgi, dpe and rpiB are introduced into escherichia coli, and finally D-glucose is isomerized to synthesize D-allose. In order to stabilize the yield, during pathway construction, six genes including FruA, ptsg, pfkB, glk, mak and pfkA are knocked out, and Galp is overexpressed, so that during D-allose synthesis, the co-utilization of various carbohydrates can be realized, glycerol is used for straingrowth, and D-glucose is used for synthesizing D-allose at a higher conversion rate.

The invention relates to a method for quickly hydrolyzing 6-deoxy-D-allose in tylosin. The method comprises the following steps: immobilizing sulfo-containing carbon-based nano solid acid in a primarymicroreactor with active functional groups, then injecting an aqueous solution of tylosin hydrochloride into the primary microreactor, and carrying out a cyclic reaction, when the residual amount ofthe tylosin is less than 4%, pumping the above-mentioned reaction solution into a secondary microreactor, fully mixing the reaction solution with a halogenated hydrocarbon organic solvent and an alkali solution, and separating out a light phase by using a centrifugal extractor to obtain high-purity 6-deoxy-D-allocatolactone. According to the method, 6-deoxy-D-allose on tylosin lactone molecules can be rapidly and efficiently hydrolyzed, and production efficiency of full-hydrolyzed byproducts is reduced, so that the purposes of optimizing a reaction route of tildipirosin, shortening a reactionperiod, reducing production cost and improving product quality are achieved.

The invention relates to a method for preparing tylosin macrolide and derivatives thereof. The method for preparing the tylosin macrolide and derivatives thereof comprises the following steps: in the presence of a fatty hydrocarbon polar solvent or aromatic hydrocarbon solvent, mixing tylosin A or salt of tylosin A and a piperidyl compound I with formic acid or aqueous solution of formic acid, so as to generate a 20-piperidyl-tylosin compound; then mixing and stirring with HCOOH or aqueous solution of HCOOH, so as to generate a 23-O-(6-deoxidation-2,3-bis-O-methyl-D-allose)-20-piperidyl-5-O-mycaminose-tylosin lactone compound; then reacting with an inorganic acid for 3-8 hours to form a 23-hydroxyl-20-piperidyl-5-O-mycaminose-tylosin lactone compound; then oxidizing to obtain an active compound by adopting a selective combined oxidizer system; reacting the active compound with a piperidyl compound II and formic acid or aqueous solution of formic acid, thus obtaining a tylosin macrolide and derivative of the tylosin macrolide. In the preparation process of the compounds, application amount of inorganic acid is reduced to 25-36 mol equivalent weight, less byproducts and high-concentration waste water are produced, and yield is increased.

[PROBLEMS] To provide the sequence of a thermotolerant L-rhamnose isomerase gene. [MEANS FOR SOLVING PROBLEMS] A DNA comprising the base sequence represented by SEQ ID NO:1. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A protein originating in Bacillus pallidus strain 14a (FERM AP-20172) and having an L-rhamnose isomerase activity. A protein having an L-rhamnose isomerase activity which is specified as having the following characteristics: optimum temperature and working temperature: showing the maximum enzymatic activity at 80° C. (the optimum temperature) and working temperature ranging from 30 to 80° C.; heat stability: concerning the effect of temperature on the enzymatic activity, being stable at up to 50° C. in the case of heating for 1 hour; and catalyzing the isomerization from D-psicose to D-allose. A method of producing D-allose which comprises treating a solution containing D-psicose with the above protein having the L-rhamnose isomerase activity as a catalyst at 35 to 80° C. to thereby convert D-psicose into D-allose.

[PROBLEMS] To control neuropathic pain produced by various mechanisms.[MEANS FOR SOLVING PROBLEMS] Disclosed is a composition for elimination, relief or reduction of neuropathic pain comprising, as an active ingredient, at least one substance selected from the group consisting of D-allose, a derivative of D-allose, D-psicose and a derivative of D-psicose preferably in an amount of 0.01 to 90% by weight. The composition may comprise a mixture of D-allose and / or a derivative thereof and D-psicose and / or a derivative thereof at a ratio of 1:1 to 10:1. The neuropathic pain may be one induced by a disease selected from the group consisting of trigeminal neuralgia, postoperative pain, periodontitis, gingivitis, gingivostomatitis, oral ulcer, herpes zoster, postherpetic neuralgia, diabetic neuritis, causalgia, phantom limb pain and malignant tumor. Also disclosed is use of the composition for elimination, relief or reduction of neuropathic pain by administering the composition to a patient with neuropathic pain in such an amount that 0.01 to 100 g of the at least one substance can be taken by the patient per day.

The invention discloses a detection method for rapidly determining the sugar mixing ratio of Dangshan pear syrup, and belongs to the technical field of food detection. The specific method is as follows: establishing a function model according to data of different soluble solid concentration degrees Brix, different sucrose doping ratios and D-allose contents of the known multiple groups of Dangshanpear syrup, obtaining a soluble solid concentration-sugar doping ratio-allose content fitting surface function equation, and obtaining a sugar doping ratio calculation equation through a transformation equation; respectively measuring the content of D-allose and the concentration degree Brix of soluble solids in the pear syrup to be measured; and substituting the D-allose content and the solublesolid concentration degree Brix into the obtained sugar mixing ratio calculation equation, and calculating to obtain the sugar mixing ratio of the pear syrup. The method is suitable for all pear syrupand pear syrup cans prepared by taking Dangshan pears as raw materials, and is not limited by a processing mode, batches of the raw materials and production places of the raw materials. The method ishigh in practicability and high in accuracy in actual product detection.

The object is to provide a novel application of D-allose (e.g., use for prevention of hypertension or hypercardia). Thus, disclosed is a composition for preventing the increase in blood pressure which comprises D-allose as an active ingredient; or a composition containing D-allose and / or a derivative thereof, preferably in an amount of 0.1 to 50% by weight. Preferably, the composition is in a form selected from the group consisting of a food additive, a food material, a beverage / food, a health beverage / food, a pharmaceutical and a feeding stuff which can be used for the prevention and treatment of a cardiovascular system disorder (e.g., hypertension, hypercardia). The increase in blood pressure may be caused by salt-sensitive hypertension. Also disclosed is use of D-allose for preventing the increase in blood pressure (excluding medical practices).

The invention discloses a culture medium used for performing selective separation on pathogenic listeria. The culture medium comprises base components including tryptone, peptone, beef extract powder, sodium chloride and disodium hydrogen phosphate, the culture medium also comprises beta-D-allose; more preferably, the culture medium also comprises acid trypaflavine, nalidixic acid and lithium chloride. Compared with a traditional enriched culture medium, the culture medium provided by the invention is increased in sensitivity by 16 percent when separating the pathogenic listeria, and a culture step is reduced, and 48 hours of culture time is shortened.

The invention relates to a chemical preparation method of L-ribose. The chemical preparation method is characterized by comprising the following steps: formulating mesityl-alpha-D-furan allose, sodium bicarbonate and distilled water into a reaction system, and adding sodium periodate for reaction at a certain temperature to obtain oxidative breakdown products; heating the oxidative breakdown products with a methanol solution of methoxyamine for a reflux reaction to obtain an oxime protected object; reacting the oxime protected object and dichlorodicyanobenzoquinone with acetonitrile-aqueous solution, then adding sodium borohydride liquid for reaction to obtain a demesityl oxime protected object; then reacting the demesityl oxime protected object with a tetrahydrofuran solution of titanium trichloride to obtain the L-ribose.

The invention relates to the medicine technology field, in particular relates to a new application of cardiac glycoside chemical compound having general formula (I) in preparing oncotherapy drug, wherein, R equals to 2-O-methyl-Beta-D-mycose, 6-deoxidation-Beta-D-gulose or 6-deoxidation-2-O-methyl-Beta-D-allose. The cardiac glycoside chemical compound can be obtained through a separation from upasin various conventional separation methods or be obtained through a synthesis or semisynthesis method.

Object: To provide a thermostable L-ribose isomerase.Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

The invention discloses a method for further synthesizing acetyl coenzyme A by reacting glycolaldehyde with 4-erythrose phosphate under the catalysis of aldolase to generate 6-allose phosphate. The method disclosed by the invention is high in catalytic rate, the theoretical carbon yield of a reaction route is 100%, carbon loss is avoided, the 4-erythritol phosphate, the enzyme and the coenzyme can be recycled, and the reaction efficiency is relatively high. The method disclosed by the invention can play more obvious advantages in the production processes of in-vitro continuous multi-enzyme catalysis, fed-batch fermentation, continuous fermentation and the like capable of controlling the substrate level.

The invention discloses a method for extracting D-allose from nut shells by a microwave radiation process. The method comprises the following steps of crushing the nut shells, carrying out microwave radiation on the crushed nut shells, carrying out solid-liquid separation on a product obtained after microwave radiation, carrying out rotary evaporation separation on a liquid obtained after solid-liquid separation, washing a viscous solid phase obtained after rotary evaporation separation with a polar solvent, and carrying out water dissolution recrystallization on insoluble substances obtainedafter washing with the polar solvent. According to the method, plant wastes, namely the nut shells, are used as raw materials, and a green reaction process is adopted, so that rare sugar, namely the D-allose, can be rapidly and efficiently extracted from the nut shells, the production cost of the D-allose is greatly reduced, and the additional value and the resource utilization rate of the nut shells are improved.