39 results about "Allose" patented technology

Disclosed is a process for producing a crystalline sugar comprising D-psicose and D-allose. Also disclosed is a process for producing the sugar.

A complex crystalline sugar comprising D-psicose and D-allose. The compositional ratio between D-psicose and D-allose in the sugar is about 1:1 to 1:4. A process for producing a complex crystalline sugar comprising D-psicose and D-allose, the process comprising producing a complex crystalline sugar comprising D-psicose and D-allose from a sugar solution containing D-psicose and p-allose and collecting the complex crystalline sugar. The solvent of the sugar solution used in the production of the complex crystalline sugar is water or a mixture of water and ethanol. The sugar solution containing D-psicose and D-allose is produced by a process comprising reacting D-psicose with L-rhamnose isomerase to convert D-psicose into D-allose. The L-rhamnose isomerase is derived from a strain (IPOD FERM BP-08593) belonging to Pseudomonas stutzeri.

The invention provides methods for the production of erythrose and/or erythritol. Preferably, the methods include the step of electrolytic decarboxylation of a ribonic acid or arabinonic acid reactant to produce erythrose. Optionally, the reactant can be obtained from a suitable hexose sugar, such as allose, altrose, glucose, fructose or mannose. The erythrose product can be hydrogenated to produce erythritol.

A method of utilizing the physiological activity of a rare saccharide, wherein physiological-activity sensitive cells are treated with the rare saccharide to modify the function of the cells. A composition containing, as an active ingredient, a rare saccharide which is introduced into physiological-activity sensitive cells and has an effect of modifying the function of the cells. The cells are human cells. The composition is a functional food, a drug, or a cosmetic. The rare saccharide is a rare saccharide belonging to aldose and/or ketose. The aldose is D-allose, and the cells are selected from the group consisting of cancer-cell proliferation inhibitory activity sensitive cells and active-oxygen production inhibitory activity sensitive cells. The ketose is D-psicose, and the cells are selected from the group consisting of chemokine secretion inhibitory activity sensitive cells, microglia migration inhibitory activity sensitive cells, and hypoglycemic activity sensitive cells.

[Problem] To provide a preservative solution for cells, tissues and organs using a rare sugar and a preservation method using the solution.

[Means for Resolution] A preservative solution for cryopreservation of animal or human organs and animal or plant tissues or cells, the solution containing a rare sugar D-allose. A preservative solution for cryopreservation of the human kidney, cells derived from animals or humans, or human or animal sperm and/or ovum and/or fermented ovum in which the cryopreservation is conducted at from −5 to 20° C. A preservative solution for cryopreservation of cells derived from animals or humans, or human or animal sperm and/or ovum and/or fermented ovum in which the cryopreservation is conducted at a temperature at which to start freezing to −196° C. A method for cryopreservation of an animal or human organ and an animal or plant tissue or cell at a low temperature, which comprises (a) refluxing or immersing a preservative solution for cryopreservation containing a rare sugar D-allose and the organ or the tissue or mixing the cell therewith, and (b) cooling the organ, the tissue or the cell to a low temperature of from −5 to 20° C.

[PROBLEMS] To find out a specific rare sugar having effects of inhibiting the proliferation of vascular endothelial cells and lumen formation and utilize these effects. To provide this rare sugar as a preventive/remedy for diseases with angiogenesis, a cosmetic or a functional food.

[MEANS FOR SOLVING PROBLEMS] A method of controlling the proliferation of vascular endothelial cells characterized by utilizing the vascular endothelial cell proliferation-controlling effect of D-mannose, D-allose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, L-sorbose, 2-deoxy-D-ribose and/or 2-deoxy-L-ribose. A method of inhibiting lumen formation of vascular endothelial cells characterized by utilizing the vascular endothelial cell lumen formation-inhibiting effect of D-allose, D-altrose, D-gulose, D-talose, L-allose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, D-ribose, L-ribose, 2-deoxy-D-ribose and/or 2-deoxy-L-ribose.

The invention relates to the medicine technology field, in particular relates to a new application of cardiac glycoside chemical compound having general formula (I) in preparing oncotherapy drug, wherein, R equals to 2-O-methyl-Beta-D-mycose, 6-deoxidation-Beta-D-gulose or 6-deoxidation-2-O-methyl-Beta-D-allose. The cardiac glycoside chemical compound can be obtained through a separation from upas in various conventional separation methods or be obtained through a synthesis or semisynthesis method.

A chewing gum base comprises food acceptable substituted polysaccharides wherein substituents on the saccharide units in the polysaccharides produce a degree of substitution of at least I.0. The polysaccharides may have branches with an average length of 1 to 15 saccharide units per branch. The polysaccharides may be linked saccharide units such as allose, altrose, mannose, gulose, idose, galactose, 3,6 anhydro galactose, glucuronic acid, mannuronic acid, galacturonic acid, aldobiouronic acid, fucose, rhamnose, arabinose, xylose, talose, acyl substituted glucose, fructose, lactose and combinations thereof.

The present invention relates to a recombinant strain for producing an allose from a fructose, a composition for producing an allose which produces an allose from a fructose-containing raw material comprising the strain, and a method for preparing an allose using the same.

The invention discloses a method for preparing D-allose by reducing ketose by a catalytic hydrogenation process, which comprises the following steps: 1) subjecting the compound of formula (II) to reduction reaction in the presence of a transitional metal catalyst and hydrogen to obtain a compound of a formula III, wherein R1 and R2 are dihydroxyl protective groups; and 2) removing the R1 and R2 protective groups from the compound of the formula III to obtain D-allose. The method has the advantages of high stereoselectivity, high cleanness, convenience for post treatment and the like and is a preparation method suitable for industrial production.

The invention discloses a method for biosynthesizing D-allose by using D-glucose, belongs to the field of escherichia coli metabolic engineering, and aims to synthesize D-allose by using organisms. Asthe content of D-allose in nature is extremely low and the extraction rate is low, the synthesis of D-arabinose in engineering escherichia coli is a content worthy of research. Three exogenous genesgi, dpe and rpiB are introduced into escherichia coli, and finally D-glucose is isomerized to synthesize D-allose. In order to stabilize the yield, during pathway construction, six genes including FruA, ptsg, pfkB, glk, mak and pfkA are knocked out, and Galp is overexpressed, so that during D-allose synthesis, the co-utilization of various carbohydrates can be realized, glycerol is used for straingrowth, and D-glucose is used for synthesizing D-allose at a higher conversion rate.

[PROBLEMS] To provide the sequence of a thermotolerant L-rhamnose isomerase gene. [MEANS FOR SOLVING PROBLEMS] A DNA comprising the base sequence represented by SEQ ID NO:1. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A protein originating in Bacillus pallidus strain 14a (FERM AP-20172) and having an L-rhamnose isomerase activity. A protein having an L-rhamnose isomerase activity which is specified as having the following characteristics: optimum temperature and working temperature: showing the maximum enzymatic activity at 80° C. (the optimum temperature) and working temperature ranging from 30 to 80° C.; heat stability: concerning the effect of temperature on the enzymatic activity, being stable at up to 50° C. in the case of heating for 1 hour; and catalyzing the isomerization from D-psicose to D-allose. A method of producing D-allose which comprises treating a solution containing D-psicose with the above protein having the L-rhamnose isomerase activity as a catalyst at 35 to 80° C. to thereby convert D-psicose into D-allose.

[PROBLEMS] To control neuropathic pain produced by various mechanisms.

[MEANS FOR SOLVING PROBLEMS] Disclosed is a composition for elimination, relief or reduction of neuropathic pain comprising, as an active ingredient, at least one substance selected from the group consisting of D-allose, a derivative of D-allose, D-psicose and a derivative of D-psicose preferably in an amount of 0.01 to 90% by weight. The composition may comprise a mixture of D-allose and/or a derivative thereof and D-psicose and/or a derivative thereof at a ratio of 1:1 to 10:1. The neuropathic pain may be one induced by a disease selected from the group consisting of trigeminal neuralgia, postoperative pain, periodontitis, gingivitis, gingivostomatitis, oral ulcer, herpes zoster, postherpetic neuralgia, diabetic neuritis, causalgia, phantom limb pain and malignant tumor. Also disclosed is use of the composition for elimination, relief or reduction of neuropathic pain by administering the composition to a patient with neuropathic pain in such an amount that 0.01 to 100 g of the at least one substance can be taken by the patient per day.

The invention discloses a detection method for rapidly determining the sugar mixing ratio of Dangshan pear syrup, and belongs to the technical field of food detection. The specific method is as follows: establishing a function model according to data of different soluble solid concentration degrees Brix, different sucrose doping ratios and D-allose contents of the known multiple groups of Dangshanpear syrup, obtaining a soluble solid concentration-sugar doping ratio-allose content fitting surface function equation, and obtaining a sugar doping ratio calculation equation through a transformation equation; respectively measuring the content of D-allose and the concentration degree Brix of soluble solids in the pear syrup to be measured; and substituting the D-allose content and the solublesolid concentration degree Brix into the obtained sugar mixing ratio calculation equation, and calculating to obtain the sugar mixing ratio of the pear syrup. The method is suitable for all pear syrupand pear syrup cans prepared by taking Dangshan pears as raw materials, and is not limited by a processing mode, batches of the raw materials and production places of the raw materials. The method ishigh in practicability and high in accuracy in actual product detection.

The object is to provide a novel application of D-allose (e.g., use for prevention of hypertension or hypercardia). Thus, disclosed is a composition for preventing the increase in blood pressure which comprises D-allose as an active ingredient; or a composition containing D-allose and/or a derivative thereof, preferably in an amount of 0.1 to 50% by weight. Preferably, the composition is in a form selected from the group consisting of a food additive, a food material, a beverage/food, a health beverage/food, a pharmaceutical and a feeding stuff which can be used for the prevention and treatment of a cardiovascular system disorder (e.g., hypertension, hypercardia). The increase in blood pressure may be caused by salt-sensitive hypertension. Also disclosed is use of D-allose for preventing the increase in blood pressure (excluding medical practices).

The invention relates to a chemical preparation method of L-ribose. The chemical preparation method is characterized by comprising the following steps: formulating mesityl-alpha-D-furan allose, sodium bicarbonate and distilled water into a reaction system, and adding sodium periodate for reaction at a certain temperature to obtain oxidative breakdown products; heating the oxidative breakdown products with a methanol solution of methoxyamine for a reflux reaction to obtain an oxime protected object; reacting the oxime protected object and dichlorodicyanobenzoquinone with acetonitrile-aqueous solution, then adding sodium borohydride liquid for reaction to obtain a demesityl oxime protected object; then reacting the demesityl oxime protected object with a tetrahydrofuran solution of titanium trichloride to obtain the L-ribose.

Object: To provide a thermostable L-ribose isomerase.

Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.