15 results about "Human kidney" patented technology

This invention discloses the application of a reagent that inhibits ADNP gene expression or ADNP protein activity in the preparation of drugs for treating renal fibrosis, belonging to the field of biomedical technology. The application of the reagent that inhibits ADNP gene expression or ADNP protein activity in the preparation of drugs for treating renal fibrosis. This invention effectively knocks down or knocks out the ADNP gene in renal tubular epithelial cells, objectively investigates the effects of ADNP deficiency on a series of phenotypes in HK-2 cells, and confirms that ADNP can inhibit EMT and renal fibrosis in HK-2 cells. Drugs that target and knock down or knock out the ADNP gene in human kidneys can be developed to delay the progression of renal fibrosis.

The present application discloses and claims a kidney phantom consisting of a silicone rubber model featuring the anatomical shape of a human kidney, the renal artery, the renal vein, and a total of 11 tumors and 2 cysts. Each tumor is represented by a solid mass, while cysts are empty three-dimensional shapes. In order to accommodate the needs of both novice and advanced surgeons, tumors were designed with increasing surgical difficulty. The kidney phantom disclosed in the present application can be combined with a commercially available ensemble of laparoscopic tools to provide localized and remote training to surgeons and medical staff practicing laparoscopic and robotic partial nephrectomy.

The invention relates to an active polypeptide and a preparation method and application thereof, and belongs to the technical field of protein. The invention provides an active polypeptide. The amino acid sequence of the active polypeptide is as shown in SEQ ID NO. 1. Cell experiments prove that the active polypeptide has the effect of inhibiting the growth of human kidney tumor related cells, so that the effect of treating kidney cancer is achieved.

The utility model belongs to the technical field of medical equipment, and provides a home body position calculus removal guiding device which comprises a fixed base, a movable base and a guide rod. The kidney model is arranged on the fixed base, a ureter is arranged on the kidney model, and the kidney model is a transparent material model; a renal pelvis cavity, a kidney large calyx cavity and a kidney small calyx cavity are formed in the kidney model, and the ureter, the renal pelvis cavity, the kidney large calyx cavity and the kidney small calyx cavity are used for simulating the ureter, the renal pelvis, the kidney large calyx and the kidney small calyx of the human kidney respectively. The transparent kidney model is convenient for the patient to intuitively observe the movement track of the stone-like member in the kidney model under different body positions. When the kidney model is used, a patient can wear the kidney model at the preset position of the waist, and the body position is changed according to the dynamic condition of the stone-like piece in the kidney model until the stone-like piece is discharged out of the pelvis. Therefore, the cost of the body position drainage bed for the patient is reduced, the hospital return frequency of the patient is reduced, and the body position calculus removing efficiency of the patient is improved.

Disclosed herein include methods and compositions for pig-to-human organ xenotransplantation using genetically modified pigs with triple-gene knockouts and human gene insertions. In some embodiments, the organ xenotransplantation is a pig-to-human kidney xenotransplantation demonstrating the restoration of renal function. Also disclosed are methods and compositions for preventing acute rejection and / or reactivation of porcine herpesvirus, which contributes to advancing xenotransplantation as a viable solution to the global organ shortage.

Provided herein is an adeno-associated virus susceptible cell strain and its application. Specifically, provided herein is an adeno-associated virus susceptible cell strain, and the application of the cell strain. The cell strain is human kidney epithelial cell 293T-AH10 cell with preservation number CCTCC NO: C202331, which moderately and stably expresses three genes of adeno virus (Ad5): E4 gene (Orf6), E2A gene and VARNA gene, and is highly susceptible to adeno-associated virus (AAV) of different serotypes, and can be used for infection titer quantitative test of AAV.

The present application belongs to the field of biomedical technology, and relates to a construction method of an experimental animal model and a constructed experimental model, in particular to a method for constructing a kidney humanized mouse model based on human cells and a constructed kidney humanized mouse model. The construction method comprises the following steps: injecting mesenchymal stem cells, human umbilical vein endothelial cells and human renal tubular epithelial cells separated from urine into immunodeficient mice subcutaneously or subcapsularly to obtain a humanized kidney vascular unit mouse model communicating with the host. The model construction has the following characteristics: the cells used are derived from urine, have the characteristics of sufficient clinical source and convenient sampling, and the kidney source cells derived from urine can represent a unique individual alone, can be used to establish an individualized kidney humanized mouse model based on patients, and have the potential to be applied to individualized precision medical research; the model can form a functional human kidney vascular unit in mice, and has great application prospects in individualized precision medical treatment of kidney disease and human kidney specific virus infection research.

The application belongs to the technical field of biology, and discloses a culture method for large-scale human kidney organoids. The application discloses a culture method for large-scale kidney organoids, which comprises the following steps: using a culture solution to induce culture of stem cells to generate developing organoids; applying fluid shear stress to the developing organoids for culture, i.e. obtaining kidney organoids; the method for applying fluid shear stress comprises placing the developing organoids in a fluid perfusion system, adjusting the fluid flow rate, and making the fluid shear force be 0.02-0.3 dyn / cm 2 , or stirring or rotating a culture system containing the developing organoids. The culture method provided by the application can significantly promote targeted enrichment and maturation of distal renal units, greatly improves the physiological fidelity of the organoids, successfully remodels the metabolic microenvironment, breaks through the glycolysis development bottleneck, and realizes large-scale preparation with high molding rate and high uniformity.

COL3A1 as a marker for detecting kidney stones and its application. The application discloses a new use of COL3A1 as a specific detection marker for kidney stones, and a kit, a detection method and application for detecting kidney stones based on the expression level of COL3A1. Through cross-species transcriptome sequencing, qRT-PCR, Western blot and immunofluorescence verification of human kidney stone clinical samples and mouse kidney stone models, it is confirmed that COL3A1 is significantly up-regulated in kidney tissues of kidney stone lesions, and can be used as a molecular marker for distinguishing kidney stone patients from healthy people. The detection method provided by the application has high specificity and sensitivity, and can realize early screening, auxiliary diagnosis, disease monitoring and recurrence risk assessment of kidney stones, and provides a new molecular target and non-invasive detection scheme for clinical diagnosis of kidney stones.

This invention discloses an anti-Vibrio polypeptide, Lvvibriocin-GK, and its applications, the amino acid sequence of which is shown in SEQ ID NO. 01. This invention is a positively charged cationic, highly effective anti-Vibrio polypeptide that exhibits significant antibacterial effects against common aquatic pathogens such as Vibrio. Furthermore, Lvvibriocin-GK showed no cytotoxic effects on zebrafish embryonic fibroblast cell line (ZF4) and human kidney epithelial cell line (HEK-293T); its antibacterial activity is unaffected by high temperature, salt ion concentration, or pH changes, demonstrating good environmental stability.

This invention relates to the fields of regenerative medicine, disease models, and drug screening, and particularly to a high-throughput method and application for constructing human kidney organoids that simulate the pathological characteristics of lupus nephritis. The kidney organoids are mixed and cultured with plasma from lupus nephritis patients in low-adsorption multi-well plates. This invention utilizes a standardized process to prepare human kidney organoids in high throughput, and then stimulates them with plasma from lupus nephritis patients, thereby rapidly and in large quantities constructing disease models in vitro that simulate the severity of individual patients' diseases and reproduce key pathological features (such as podocyte damage and inflammatory fibrosis). This platform provides a closed-loop research tool for lupus nephritis research, from "clinical samples" to "in vitro models" and then to "clinical validation."

According to the laminaria proteoglycan purified extract and the preparation method and application thereof, in the preparation process of the purified extract, an ultrasonic-assisted water extraction method and anion exchange chromatography are adopted for purification, the extraction efficiency is high, the purification effect is good, laminaria proteoglycan effective ingredients in laminaria can be effectively enriched, and the content of laminaria proteoglycan in laminaria is increased. The obtained extract is high in purity and high in activity, high-value utilization of kelp resources is achieved, kelp proteoglycan is used as a whole for extraction, purification and activity research, and the synergistic effect of kelp proteoglycan is fully exerted. In the invention, a caenorhabditis elegans model, a zebra fish model and a human renal epithelial cell (HK-2) model verify that the laminarin purified extract has a remarkable protective effect on the body injury caused by the microplastics, and a new candidate medicine and a scientific basis are provided for developing the microplastic injury protection medicine.

The invention discloses a 3D culture method of vascularized human kidney organoid and application thereof. The 3D culture method of the human kidney organoid comprises the following steps: S1, preparing a pig kidney CdECM gel solution; s2, resuspending the hPSCs by using a first differential culture medium, performing induced differentiation for 36-60 hours, and continuously culturing for 12-36 hours after half amount of the liquid is changed; replacing the culture system with a second differential culture medium, culturing for 3-7 days, and replacing half of the liquid every other day during the culture process to obtain primary human kidney organs; and S3, uniformly mixing the primary human kidney organ obtained in the step S2 and the pig kidney CdECM gel solution obtained in the step S1, standing for 10-15 minutes, transferring into a cell culture plate after gel formation, culturing for 6-8 days, and changing a half amount of the solution every other day in the period, so as to obtain the human kidney organ. According to the method, a 3D growth environment closer to the in-vivo environment is provided for kidney organ differentiation, and kidney organ blood vessel differentiation is promoted.

The invention discloses andrias davidianus bone peptide for relieving renal injury and application of the andrias davidianus bone peptide, a polypeptide sequence comprises an amino acid sequence shown in any one of SEQ ID NOs: 1-10 or an amino acid sequence shown in any one of SEQ ID NOs: 1-10, and the polypeptide can improve the renal injury. For example, hyperuricemia-induced ferroptosis and fibrosis injury of human kidney proximal tubular HK-2 cells can be relieved.

The application provides a laminarin proteoglycan purified extract and a preparation method and application thereof. In the preparation process of the purified extract, ultrasonic-assisted water extraction and anion exchange chromatography are used for purification, the extraction efficiency is high, the purification effect is good, the effective components of laminarin proteoglycan in laminaria can be effectively enriched, the obtained extract has high purity and strong activity, the high-value utilization of laminaria resources is realized, the laminarin proteoglycan is extracted and purified as a whole and activity research is conducted, and the synergistic effect is fully exerted. In the application, through the Caenorhabditis elegans model, the zebra fish model and the human kidney epithelial cell (HK-2) model, it is verified that the laminarin proteoglycan purified extract has a significant protection effect on the damage of the organism caused by microplastics, and a new candidate drug and scientific basis are provided for the development of a microplastic damage protection drug.