24 results about "Human kidney" patented technology

The invention belongs to the technical field of biomedicine, and discloses an immortalized cell line for human kidney suspected cell carcinoma as well as a culture method and application of the immortalized cell line. The immortalized cell line for the human kidney suspected cell carcinoma, disclosed by the invention, is named as a human kidney suspected cell carcinoma cell line Loya a-710 (Homo sapiens), the classification name of the Latin name of the immortalized cell line is Chromophore sensor cell carcino: Loya a-710, and the preservation number of the immortalized cell line is CCTCC (China Center for Type Culture Collection) NO: C2025123. The human kidney suspected cell carcinoma cell line Loya-710 disclosed by the invention is used as an in-vitro model to rapidly grow in tissue culture, and mitochondrial mutation characteristics, a vesicle structure and a classical immunohistochemical marker of ChRCC are reserved. The invention provides a precious tool for further research on ChRCC heredity, molecules and biological characteristics, and also provides a powerful new model for mitochondrial diseases.

Described herein are methods for treating antibody mediated rejection (ABMR), especially chronic active ABMR (cABMR), of transplanted organs using clazakizumab. Human kidney transplant recipients with biopsy-proven cABMR, transplant glomerulopathy and who are donor-specific antibody positive showed stabilization of renal function and lowered DSA levels following clazakizumab treatment. The estimated glomerular filtration rate of the patients at six, 12 or even 18 months were stabilized, inflammatory markers of cABMR were reduced or stabilized, and inflammatory blood markers were reduced, since clazakizumab treatment.

The invention belongs to the technical field of biological medicine, and particularly relates to an in-vitro fusion technology for multi-lineage human kidney organs and application thereof. The invention provides a method for realizing culture of multilineage kidney organs by fusing kidney units and ureteral bud organs in vitro. According to the in-vitro fusion technology of the kidney organoid reported by the invention, two organoid with different pedigree sources, namely the kidney unit and the ureteral bud, can be efficiently fused, a UB tubular structure is integrated into the kidney unit organoid, and the UB tubular structure starts from a single ureteral bud and is wrapped by kidney unit precursor cells in a similar in-vivo development process, and finally develops into a kidney with glomerulus and a plurality of kidney cells. The multi-lineage renal organ comprises a catheter, a proximal tube, a marrow tab thin section, a distal tube, a connecting tube and a ureteral bud with a single outlet. A framework is provided for an engineered renal unit and a smooth collection system, and the method is an important step for generating the functional renal tissue from the beginning.

This invention discloses the application of a reagent that inhibits ADNP gene expression or ADNP protein activity in the preparation of drugs for treating renal fibrosis, belonging to the field of biomedical technology. The application of the reagent that inhibits ADNP gene expression or ADNP protein activity in the preparation of drugs for treating renal fibrosis. This invention effectively knocks down or knocks out the ADNP gene in renal tubular epithelial cells, objectively investigates the effects of ADNP deficiency on a series of phenotypes in HK-2 cells, and confirms that ADNP can inhibit EMT and renal fibrosis in HK-2 cells. Drugs that target and knock down or knock out the ADNP gene in human kidneys can be developed to delay the progression of renal fibrosis.

The present application discloses and claims a kidney phantom consisting of a silicone rubber model featuring the anatomical shape of a human kidney, the renal artery, the renal vein, and a total of 11 tumors and 2 cysts. Each tumor is represented by a solid mass, while cysts are empty three-dimensional shapes. In order to accommodate the needs of both novice and advanced surgeons, tumors were designed with increasing surgical difficulty. The kidney phantom disclosed in the present application can be combined with a commercially available ensemble of laparoscopic tools to provide localized and remote training to surgeons and medical staff practicing laparoscopic and robotic partial nephrectomy.

The invention relates to an active polypeptide and a preparation method and application thereof, and belongs to the technical field of protein. The invention provides an active polypeptide. The amino acid sequence of the active polypeptide is as shown in SEQ ID NO. 1. Cell experiments prove that the active polypeptide has the effect of inhibiting the growth of human kidney tumor related cells, so that the effect of treating kidney cancer is achieved.

The utility model belongs to the technical field of medical equipment, and provides a home body position calculus removal guiding device which comprises a fixed base, a movable base and a guide rod. The kidney model is arranged on the fixed base, a ureter is arranged on the kidney model, and the kidney model is a transparent material model; a renal pelvis cavity, a kidney large calyx cavity and a kidney small calyx cavity are formed in the kidney model, and the ureter, the renal pelvis cavity, the kidney large calyx cavity and the kidney small calyx cavity are used for simulating the ureter, the renal pelvis, the kidney large calyx and the kidney small calyx of the human kidney respectively. The transparent kidney model is convenient for the patient to intuitively observe the movement track of the stone-like member in the kidney model under different body positions. When the kidney model is used, a patient can wear the kidney model at the preset position of the waist, and the body position is changed according to the dynamic condition of the stone-like piece in the kidney model until the stone-like piece is discharged out of the pelvis. Therefore, the cost of the body position drainage bed for the patient is reduced, the hospital return frequency of the patient is reduced, and the body position calculus removing efficiency of the patient is improved.

Disclosed herein include methods and compositions for pig-to-human organ xenotransplantation using genetically modified pigs with triple-gene knockouts and human gene insertions. In some embodiments, the organ xenotransplantation is a pig-to-human kidney xenotransplantation demonstrating the restoration of renal function. Also disclosed are methods and compositions for preventing acute rejection and / or reactivation of porcine herpesvirus, which contributes to advancing xenotransplantation as a viable solution to the global organ shortage.

Provided herein is an adeno-associated virus susceptible cell strain and its application. Specifically, provided herein is an adeno-associated virus susceptible cell strain, and the application of the cell strain. The cell strain is human kidney epithelial cell 293T-AH10 cell with preservation number CCTCC NO: C202331, which moderately and stably expresses three genes of adeno virus (Ad5): E4 gene (Orf6), E2A gene and VARNA gene, and is highly susceptible to adeno-associated virus (AAV) of different serotypes, and can be used for infection titer quantitative test of AAV.

According to the human kidney physiological age prediction method based on machine learning, five blood detection indexes of creatinine, albumin, urea, uric acid and total protein closely related to the kidney are collected in a targeted manner, and computer model modeling is performed on male and female data according to kidney health data; and constructing a computer model for predicting the physiological ages of the kidneys of the male and female human bodies to evaluate the physiological ages of the kidneys of the individual bodies with different genders. According to the human kidney physiological age prediction method based on machine learning, core data only uses data indexes of creatinine, albumin, urea, uric acid and total protein related to the human kidney for modeling, a physiological age model of the human kidney is constructed, and the physiological age of the human kidney can be evaluated in a targeted mode; moreover, according to the human kidney physiological age prediction method based on machine learning, modeling is performed on the male and the female based on the data difference of the male and the female genders, so that the prediction result is more accurate.

The present application belongs to the field of biomedical technology, and relates to a construction method of an experimental animal model and a constructed experimental model, in particular to a method for constructing a kidney humanized mouse model based on human cells and a constructed kidney humanized mouse model. The construction method comprises the following steps: injecting mesenchymal stem cells, human umbilical vein endothelial cells and human renal tubular epithelial cells separated from urine into immunodeficient mice subcutaneously or subcapsularly to obtain a humanized kidney vascular unit mouse model communicating with the host. The model construction has the following characteristics: the cells used are derived from urine, have the characteristics of sufficient clinical source and convenient sampling, and the kidney source cells derived from urine can represent a unique individual alone, can be used to establish an individualized kidney humanized mouse model based on patients, and have the potential to be applied to individualized precision medical research; the model can form a functional human kidney vascular unit in mice, and has great application prospects in individualized precision medical treatment of kidney disease and human kidney specific virus infection research.

The application belongs to the technical field of biology, and discloses a culture method for large-scale human kidney organoids. The application discloses a culture method for large-scale kidney organoids, which comprises the following steps: using a culture solution to induce culture of stem cells to generate developing organoids; applying fluid shear stress to the developing organoids for culture, i.e. obtaining kidney organoids; the method for applying fluid shear stress comprises placing the developing organoids in a fluid perfusion system, adjusting the fluid flow rate, and making the fluid shear force be 0.02-0.3 dyn / cm 2 , or stirring or rotating a culture system containing the developing organoids. The culture method provided by the application can significantly promote targeted enrichment and maturation of distal renal units, greatly improves the physiological fidelity of the organoids, successfully remodels the metabolic microenvironment, breaks through the glycolysis development bottleneck, and realizes large-scale preparation with high molding rate and high uniformity.

COL3A1 as a marker for detecting kidney stones and its application. The application discloses a new use of COL3A1 as a specific detection marker for kidney stones, and a kit, a detection method and application for detecting kidney stones based on the expression level of COL3A1. Through cross-species transcriptome sequencing, qRT-PCR, Western blot and immunofluorescence verification of human kidney stone clinical samples and mouse kidney stone models, it is confirmed that COL3A1 is significantly up-regulated in kidney tissues of kidney stone lesions, and can be used as a molecular marker for distinguishing kidney stone patients from healthy people. The detection method provided by the application has high specificity and sensitivity, and can realize early screening, auxiliary diagnosis, disease monitoring and recurrence risk assessment of kidney stones, and provides a new molecular target and non-invasive detection scheme for clinical diagnosis of kidney stones.

This invention discloses an anti-Vibrio polypeptide, Lvvibriocin-GK, and its applications, the amino acid sequence of which is shown in SEQ ID NO. 01. This invention is a positively charged cationic, highly effective anti-Vibrio polypeptide that exhibits significant antibacterial effects against common aquatic pathogens such as Vibrio. Furthermore, Lvvibriocin-GK showed no cytotoxic effects on zebrafish embryonic fibroblast cell line (ZF4) and human kidney epithelial cell line (HEK-293T); its antibacterial activity is unaffected by high temperature, salt ion concentration, or pH changes, demonstrating good environmental stability.

This invention relates to the fields of regenerative medicine, disease models, and drug screening, and particularly to a high-throughput method and application for constructing human kidney organoids that simulate the pathological characteristics of lupus nephritis. The kidney organoids are mixed and cultured with plasma from lupus nephritis patients in low-adsorption multi-well plates. This invention utilizes a standardized process to prepare human kidney organoids in high throughput, and then stimulates them with plasma from lupus nephritis patients, thereby rapidly and in large quantities constructing disease models in vitro that simulate the severity of individual patients' diseases and reproduce key pathological features (such as podocyte damage and inflammatory fibrosis). This platform provides a closed-loop research tool for lupus nephritis research, from "clinical samples" to "in vitro models" and then to "clinical validation."

Systems and methods for culture of human kidney organoids as a model system for characterizing kidney pathologies and implementing therapeutics for conditions that affect the kidney. A model system for characterization of a pathophysiology includes a culture of human kidney organoids and an organoid culture medium. The organoids may include one or more genome edits as part of an approach for studying genetic factors involved with a pathological process. The culture may include one or more other factors, such as pathological agents and / or anti-pathological agents, for development and evaluation of therapeutics. The approaches may be used for implementation of compositions and methods for virally transducing the kidney or a subset of cells or cell types thereof.

According to the laminaria proteoglycan purified extract and the preparation method and application thereof, in the preparation process of the purified extract, an ultrasonic-assisted water extraction method and anion exchange chromatography are adopted for purification, the extraction efficiency is high, the purification effect is good, laminaria proteoglycan effective ingredients in laminaria can be effectively enriched, and the content of laminaria proteoglycan in laminaria is increased. The obtained extract is high in purity and high in activity, high-value utilization of kelp resources is achieved, kelp proteoglycan is used as a whole for extraction, purification and activity research, and the synergistic effect of kelp proteoglycan is fully exerted. In the invention, a caenorhabditis elegans model, a zebra fish model and a human renal epithelial cell (HK-2) model verify that the laminarin purified extract has a remarkable protective effect on the body injury caused by the microplastics, and a new candidate medicine and a scientific basis are provided for developing the microplastic injury protection medicine.

The invention discloses a 3D culture method of vascularized human kidney organoid and application thereof. The 3D culture method of the human kidney organoid comprises the following steps: S1, preparing a pig kidney CdECM gel solution; s2, resuspending the hPSCs by using a first differential culture medium, performing induced differentiation for 36-60 hours, and continuously culturing for 12-36 hours after half amount of the liquid is changed; replacing the culture system with a second differential culture medium, culturing for 3-7 days, and replacing half of the liquid every other day during the culture process to obtain primary human kidney organs; and S3, uniformly mixing the primary human kidney organ obtained in the step S2 and the pig kidney CdECM gel solution obtained in the step S1, standing for 10-15 minutes, transferring into a cell culture plate after gel formation, culturing for 6-8 days, and changing a half amount of the solution every other day in the period, so as to obtain the human kidney organ. According to the method, a 3D growth environment closer to the in-vivo environment is provided for kidney organ differentiation, and kidney organ blood vessel differentiation is promoted.

The invention relates to the field of biotechnology and environmental toxicology research, and particularly discloses a construction method of a GR reporter gene stably transfected cell strain based on human renal epithelial cells HEK-293T, and the construction method comprises the following steps: S1, constructing a GAL 4 / USA-ffucc-GFP-Puro reporter gene vector; s2, a GAL4DBD-GRLBD-BSD gene overexpression vector is constructed, and the GAL4DBD- And S3, carrying out lentivirus transduction and cell strain drug screening on the 293T cell, namely introducing a GAL4 / USA-ffuc-GFP-Puro reporter gene vector and a GAL4DBD-GRLBD-BSD gene overexpression vector into the cell, and carrying out screening, so as to construct the HEK-293T-GAL4 / USA-ffuc-GFP-GAL4DBD-GRLBD monoclonal stably transfected cell strain. The stably transfected cell strain can be used for evaluating the transcriptional activation or inhibition effect of an exogenous compound on a GR receptor, so that the stably transfected cell strain has important application prospects in the aspects of GR receptor function research, drug activity test, environmental endocrine disrupter (EDCs) screening and the like.

The present invention belongs to the field of biopharmaceutical technology, and specifically relates to a method and application for preparing cucurbitacin B derivatives by strong acid dehydration and peroxyacid oxidation. The present invention utilizes strong acid to catalyze the dehydration of cucurbitacin B and peroxyacid to oxidize the carbon-carbon double bond of cucurbitacin B, provides a variety of structural modification methods for cucurbitacin B, and synthesizes a series of novel cucurbitacin B derivatives. Subsequently, by using human breast cancer MCF-7 cells and human kidney epithelial HEK-293 cells to verify its proliferation inhibition effect and specificity, it was found that the anti-tumor activity of the compound was retained, and the selectivity index was improved compared to cucurbitacin B, which is worthy of further study.

The present invention discloses a high-throughput human kidney organoid induction differentiation method, including: adding ROCK pathway inhibitor to human induced pluripotent stem cells for 18 to 24 hours; replacing with GSK-3 inhibitor for 1 to 4 days; replacing with culture medium B containing GSK-3 inhibitor, FGF-9 and Heparin for 1 to 3 days; carrying out high-throughput 3D culture, efficiently obtaining a large number of organoid spheres; after treatment with GSK-3 inhibitor, culture medium B containing FGF-9 and Heparin for 1 to 6 days; replacing with culture medium B without addition for 1 to 10 days to obtain mature kidney organoids. The method is simple to operate, highly targeted, improves the efficiency and differentiation stability of induced differentiation, reduces the time required for differentiation and maturation, increases the proliferation efficiency of kidney organoids, and solves the problem that the differentiation efficiency in conventional methods is uneven and the operation is cumbersome.

Microfluidic systems, kits, and methods for characterization of polycystic kidney disease (PKD) are described. In an embodiment, the microfluidic system includes a flow device comprising an inlet, an outlet, and a channel comprising a functionalized site configured for cell culture. In an embodiment, a genetically modified (GM) human kidney organoid is cultured at the functionalized site, optionally in the presence of a fluidic flow, to produce PKD cysts for use as a model system for characterization of mechanisms of PKD onset, progression, diagnosis, and response to treatment.

The invention discloses andrias davidianus bone peptide for relieving renal injury and application of the andrias davidianus bone peptide, a polypeptide sequence comprises an amino acid sequence shown in any one of SEQ ID NOs: 1-10 or an amino acid sequence shown in any one of SEQ ID NOs: 1-10, and the polypeptide can improve the renal injury. For example, hyperuricemia-induced ferroptosis and fibrosis injury of human kidney proximal tubular HK-2 cells can be relieved.

The application provides a laminarin proteoglycan purified extract and a preparation method and application thereof. In the preparation process of the purified extract, ultrasonic-assisted water extraction and anion exchange chromatography are used for purification, the extraction efficiency is high, the purification effect is good, the effective components of laminarin proteoglycan in laminaria can be effectively enriched, the obtained extract has high purity and strong activity, the high-value utilization of laminaria resources is realized, the laminarin proteoglycan is extracted and purified as a whole and activity research is conducted, and the synergistic effect is fully exerted. In the application, through the Caenorhabditis elegans model, the zebra fish model and the human kidney epithelial cell (HK-2) model, it is verified that the laminarin proteoglycan purified extract has a significant protection effect on the damage of the organism caused by microplastics, and a new candidate drug and scientific basis are provided for the development of a microplastic damage protection drug.