637 results about "Regenerative medicine" patented technology

The invention provides an anticoagulant coating for medical instruments, which can be used in the field of regenerative medical instruments. The prepared anticoagulant coating has short-term and long-term anticoagulant performance, and in addition, the thrombin responsive nanoparticles wrapped in the hydrogel coating can selectively release a factor Xa (FXa) inhibitor apixaban according to needs, so that thrombosis is inhibited in the middle stage of implantation. In-vitro and in-vivo experimental evidences show that after the material is implanted for a long time, an organized endodermis can be seen on the surface of the material, and the material has a special antithrombotic characteristic. The dynamic adjusting system has a multi-layer protection mechanism, and a new thought can be provided for designing the surface of a contact blood material adapting to environmental conditions.

The present invention provides a method and culture medium for promoting adipogenic differentiation of stem cells in vitro, and belongs to the field of stem cell technology. The present invention inhibits the expression of ENST00000566297.1 gene by designing specific siRNA, thereby significantly promoting the adipogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs). The experimental results show that after transfection of siRNA, the intracellular lipid droplet content is significantly increased, and the adipogenic differentiation efficiency is significantly improved. In addition, the present invention also provides an optimized culture medium formula, including transfection culture medium, adipogenic induction liquid A and adipogenic induction liquid B. This culture medium can better support the adipogenic differentiation process of adipose-derived mesenchymal stem cells, thereby providing new strategies and technical support for adipose tissue engineering and regenerative medicine.

The invention belongs to the technical field of regenerative medicine, and provides a circular cornea embedded micro lens, which comprises two areas, namely a first correction area and a plano area, preferably comprises three areas, namely two vision correction areas and a plano area, the two vision correction areas have the vision correction effect, the diopter of the plano area is 0D, and the diopter of the first correction area is 0 D, and the diopter of the plano area is 0 D; wherein the flat light area is provided with a through hole, and the applicant summarizes and finds that the through hole is formed in the center of the cornea embedded microlens, so that the phenomena of nebula and turbidity of the cornea after operation can be reduced. After the cornea embedded microlens provided by the invention is implanted, a patient can clearly see near objects and distant objects, so that clear vision is realized. The invention further provides a preparation method and application of the cornea embedded microlens.

Provided is a use of Rhodococcus ruber cell wall skeleton in promoting the proliferation of stem cells, promoting the growth of stem cells, promoting the differentiation of stem cells, promoting the migration of stem cells, and improving the survival rate of stem cells; the stem cells are selected from: adult stem cells, iPSCs, and mesenchymal stem cells.

The invention discloses an extracellular matrix-imitated anti-inflammatory nerve repair-promoting conductive hydrogel as well as a preparation method and application thereof, and belongs to the technical field of high polymer materials. The preparation method of the conductive hydrogel comprises the following steps: preparing an MXene nanosheet dispersion liquid, and sequentially adding polyphenol and magnesium ions into the MXene nanosheet dispersion liquid to obtain a modified nanosheet solution; mixing the modified chitosan with polyvinyl alcohol to obtain a solution A; mixing and reacting the modified nanosheet solution with phenylboronic acid modified gelatin to obtain a solution B; mixing the solution A and the solution B for reaction, refrigerating at low temperature, and unfreezing to obtain the conductive hydrogel. The hydrogel prepared by the invention has excellent conductivity, and the obtained conductive hydrogel has the functions of resisting inflammation and promoting nerve repair. The bionics method not only improves the diversity and functionality of biological materials, but also provides new possibility for future tissue engineering and regenerative medicine.

The invention belongs to the technical field of regenerative medicine, and provides a circular partitioned micro lens for cornea implantation, which comprises two areas, namely a first correction area and a plano area, preferably comprises three areas, namely two vision correction areas and a plano area, the two vision correction areas have the vision correction effect, and the plano area has the vision correction effect. The diopter of the plano area is 0D, the plano area is provided with a through hole, and the applicant summarizes and finds that the through hole is formed in the center of the micro lens for the partitioned cornea implantation, so that the phenomena of nebula and turbidity of the cornea after operation can be reduced. After the partitioned micro lens for cornea implantation provided by the invention is implanted, a patient can clearly see near objects and distant objects, so that clear vision is realized. The invention further provides application of the partitioned micro lens for cornea implantation in vision correction.

Provided herein are devices and methods that represent a dual function approach to tissue engineering and regenerative medicine, particularly for bone fracture or segmental defect healing. The devices include an implantable substrate; a first active composition, and a second active composition that comprises one or more oxygen-modulating compositions. The incorporation of dual bioactive compositions on the substrate offers a versatile platform for manipulating cells and modulating the local tissue oxygen environment at the site of implantation.

The present application relates to polymers, conjugates, hydrogels, and kits comprising 2- methacryloyloxyethyl phosphorylcholine (MPC) and / or copolymers comprising MPC, and collagen or a collagen-like peptide (CLP). The polymers, conjugates, hydrogels, and kits may be used as fillers and sealants for various applications, for example for treating a wound or tissue injury, such as corneal injury or perforation. Devices and systems for the administration of the polymers, conjugates, and hydrogels are also disclosed.

The present invention discloses a method for enhancing cardiomyocyte production from induced pluripotent stem cells (iPSCs) by adjusting the culture medium exchange process during the transition from WNT activation to inhibition for differentiation. Instead of completely replacing the culture medium, this innovative technique involves partially changing the medium, which allows for a controlled exposure to WNT activators concurrent with the introduction of WNT inhibitors. This key modification in the protocol markedly increases the consistency and efficiency of cardiomyocyte generation, reducing variability between batches. By fine-tuning the medium exchange process, this method streamlines the creation of cardiomyocytes for use in regenerative medicine, emphasizing the critical adjustment phase within the culture conditions.

The invention belongs to the technical field of biomedical materials and regenerative medicine, and relates to collagen-based injectable fiber hydrogel as well as a preparation method and application thereof. According to the injectable fiber hydrogel, methacrylated collagen and dithiothreitol are subjected to a thiol-ene click reaction rapidly under the action of a photoinitiator through illumination, a primary cross-linked network is formed, and the injection and in-situ gel forming requirements are met; then under the conditions that the pH is 6.5-8.0 and the temperature is 30-39 DEG C, collagen molecules are self-assembled into a nanofiber structure, and a bionic network similar to a natural extracellular matrix is obtained. The hydrogel disclosed by the invention has the advantages of rapid gelation, structural biomimetic property and biological activity; residual sulfydryl endows the hydrogel with active oxygen scavenging capacity, so that oxidative stress can be relieved, and immune response can be regulated; the fibrotic structure promotes cell adhesion, migration and tissue regeneration.

The invention belongs to the technical field of cross culture of biological materials and organoids, and particularly relates to an ECM protein for three-dimensional culture of vascular organoids. HA-; the invention relates to a preparation method and application of Gel composite hydrogel. In order to solve the problems that in the 3D culture process of existing cell spheres or organoid spheres, matrigel derived from mouse sarcoma seriously depends on, components are undefined, the price is high, and immunogenicity exists, the composite gel with the definite components is provided and used for replacing Matrigel to conduct 3D culture of vascular organoids. The composite hydrogel prepared by the invention has the characteristics of short gelation time, good biocompatibility, adjustable gel components and the like under the synergistic effect of all the raw materials, can solve the problem of matrigel used for 3D culture of organoids, can replace the potential of matrigel in the future, reduces the price of 3D culture cell scaffold materials, and has broad application prospects. Meanwhile, the method can also be used in the regenerative medicine field of other two-dimensional or three-dimensional cell and organoid culture.

The invention discloses stem cell freeze-dried powder for promoting skin healing and a preparation method of the stem cell freeze-dried powder. The freeze-dried powder comprises an effective component, a freeze-drying protective agent group and a polymer protective agent group, the effective component is umbilical cord mesenchymal stem cell conditioned medium freeze-dried supernatant, the protective agent group is selected from one or more of cane sugar, trehalose, lactose and mannitol, and the polymer protective agent group is selected from one or more of polyvinylpyrrolidone (PVP) and glucan. The preparation method comprises the following steps: collection of a conditioned culture medium, preparation of a freeze-drying protective agent solution, mixing treatment, low-temperature pre-freezing and freeze-drying, collection and packaging of freeze-dried powder and the like. The method can effectively retain stem cell-derived active factors and enhance the stability and redissolvability of the freeze-dried powder, and is suitable for skin wound repair, regenerative medicine and other scenes.

In various embodiments, the present invention provides well-defined biodegradable poly(lactone-b-propylene fumarate) diblock and triblock polymers formed using a novel one-pot, scalable ring-opening block-order copolymerization (ROBOCOP) technique that utilizes magnesium 2,6-di-tert-butyl-4-methylphenoxide (Mg(BHT)2(THF)2) to “switch” from the ROP of cyclic esters to the ROCOP of maleic anhydride (MAn) and propylene oxide (PO) to produce PPF based block copolymers for application in additive manufacturing and patient specific regenerative medicine. These block copolymers are fully resorbable and can be photochemically crosslinked in a number of applications, including 3D printing. By adding the lactone block to the PPF polymer, the viscosity of the resulting block copolymer at working temperatures can be precisely controlled and the quantity of the reactive diluent in printable resins can be reduced or eliminated.

A construction and use of an environmentally adaptive co-regulated mRNA nanodelivery system. By incorporating EACR molecules into mRNA nanoparticles, the microenvironment is remodeled to be suitable for robust and sustained mRNA expression, while tissue damage associated with the self-immunogenicity of mRNA drugs is avoided. The nanodelivery system is compatible with ionizable lipid nanoparticles, cationic liposomes, cationic nanoemulsions, and polymeric nanoparticles. The EACR molecules include: anti-inflammatory drugs, tyrosine kinases / adaptors, JAK / STAT and MAPK pathway inhibitors, nutrients and metabolites, membrane transporters / ion channels, phosphodiesterase and cellular stress inhibitors, and natural viral proteins. The therapeutic mRNAs may encode tumor, viral, or bacterial antigens, immunomodulatory factors, therapeutic antibodies, or functional proteins / enzymes, and can play a role in the fields of regenerative medicine, protein supplementation / replacement therapy, targeted gene editing, and immunotherapy.

The invention discloses a culture method of high-activity human urine-derived stem cells, which relates to the technical field of biological medicine, and is technically characterized by comprising the following steps: collecting urine samples of pregnant and lying-in women, coating a culture plate with gelatin, inducing adherence, performing passage amplification, performing immunofluorescence identification, performing function detection and other optimized conditions, and separating and culturing the stem cells. Compared with urine-derived stem cells of normal adults, the stem cells derived from pregnant women have stronger activity, higher dryness and faster multiplication capacity. The invention provides a novel and superior cell source for tissue engineering and regenerative medicine.

The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.

The invention relates to a mesenchymal stem cell immunomodulatory function enhancing technology based on gene editing. The invention relates to the field of biomedical engineering and regenerative medicine, and particularly provides a method for enhancing the immune regulation function of mesenchymal stem cells through a CRISPR-Cas9 gene editing technology, and aims at up-regulating IDO1, IL-10 and other key genes in a targeted manner and remarkably improving the excessive immune response inhibition capability of MSCs. The technology can be used for developing high-efficiency and low-toxicity novel cell drugs for treating autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus.

The invention belongs to the technical field of biological analysis and evaluation, and particularly relates to a stem cell differentiation process multi-modal image fusion and intelligent evaluation method, which comprises a stem cell differentiation process multi-modal image fusion method: on the basis of constructing a multi-modal near-physiological mechanics microenvironment, carrying out image analysis by adopting the multi-modal image fusion method; an intelligent evaluation method for the stem cell differentiation process: developing an intelligent evaluation method based on deep learning driving on the basis of constructing a multi-modal near-physiological mechanical microenvironment, and revealing a mechanical-epigenetic regulation mechanism; the synergistic application of the two solves the core observation and regulation problems in stem cell differentiation research, and provides an industrialized intelligent platform for regenerative medicine. According to the method, the data integrity can be improved, the resolution limitation is broken through, the experiment process is accelerated, accurate mechanism analysis can be realized, automatic decision making is promoted, and the research and development cost is reduced.

The invention provides a culture medium composition and a method for preparing mesenchymal stem cells. The culture medium combination comprises a culture medium A, a culture medium B and a differential culture medium, the culture medium A comprises a basic culture medium and a first small molecule compound combination, the culture medium B comprises a basic culture medium and a second small molecule compound combination, and the differential culture medium comprises a basic culture medium and a third small molecule compound combination. According to the culture medium and the method, the MSC can be prepared through ES or iPSC, and the culture medium and the method can be widely applied to the fields of regenerative medicine, tissue engineering, immunotherapy and the like.

The invention relates to a nerve repair guide catheter, a preparation method and application, an electrostatic spinning technology is adopted to construct a nanofiber membrane which is directionally and orderly arranged so as to guide nerve axons to directionally grow, and cell energy metabolism is promoted and tissue repair and regeneration are accelerated through metabolism and conversion of degradation products. Therefore, the nerve injury repairing effect better than that of traditional matrix materials without biological activity, such as PCL, is achieved. According to the invention, a novel solution is provided for solving the problem of insufficient biological activity of a traditional nerve conduit material by constructing a degradable high polymer material with biological activity. Therefore, the regeneration efficiency of peripheral nerve injury repair is improved, and the application prospect of the bioactive material in the fields of tissue engineering and regenerative medicine is expanded.

The technology disclosed herein relates to compositions and methods for improving the electrophysiological maturity of in vitro-differentiated cardiomyocytes. Media that promote electrophysiological maturation are described, as well as methods for the use of such media and the electrophysiologically matured cardiomyocytes in e.g., transplant and regenerative medicine procedures. The technology described herein is directed to cardiomyocyte maturation medium. Also described herein are associated compositions, kits, and matured cardiomyocytes. The cardiomyocyte maturation medium disclosed here can be used to modulate at least one electrophysiologic property of a cardiomyocyte. The electrophysiologically matured cardiomyocytes can engraft into cardiac tissue and can reduce or inhibit engraftment arrythmia in a subject.

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The invention provides a treatment system for promoting dental pulp repair by using oral stem cells, and relates to the technical field of oral treatments.According to the treatment system, through the synergistic effect of a dynamic gene regulation and control module, a self-adaptive intelligent support and a biological digital fusion interface, the dental pulp repair is promoted through the dynamic gene regulation and control module and the self-adaptive intelligent support; the dynamic gene regulation and control module adopts a light-operated CRISPR system, carries out real-time gene editing on dental pulp stem cells and carries out dynamic adjustment according to regeneration stage requirements, and the self-adaptive intelligent stent is based on a 3D printing technology, is manufactured in combination with a patient tooth digital model and can dynamically release growth factors and optimize a microenvironment; through combination of personalized design and dynamic regulation and control, adaptive adjustment can be performed according to specific requirements of patients, so that not only is the repair efficiency of dental pulp tissues improved, but also the success rate of functional reconstruction is increased, more efficient and accurate treatment selection is provided for the patients suffering from dental pulp injury, and personalized development of oral regenerative medicine is promoted.

The invention is suitable for the technical field of bioengineering, and provides an endometrial organ model based on a micro-fluidic chip technology and a construction method thereof. According to the invention, an endometrial organ dynamic culture system based on a micro-fluidic chip technology is constructed, and the system can accurately simulate the in-vivo physiological microenvironment of endometrium, including key factors such as periodic mechanical stress, fluid shear force and intercellular interaction. Meanwhile, by comparing traditional 3D static culture with a micro-fluidic chip dynamic culture system, the remarkable advantages of dynamic culture in the aspects of organoid morphology, function and the like are defined, and the unique value of a micro-fluidic technology in construction of a highly-bionic endometrial organoid model is deeply reflected. An endometrial organ model constructed by the dynamic culture system can provide a reliable in-vitro platform closer to an in-vivo real state for pathogenesis research, drug screening, regenerative medicine research and the like of endometrial related diseases, and basic research and transformation application in related fields are assisted.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The invention relates to the field of gel matrices and scaffold development; in particular for use in the field of tissue engineering and regenerative medicine (TERM) and provides for a water insoluble, thermo- stable, crosslinked gelatin matrix. The fabricated matrices of the invention can be applied directly into or onto the defect and the regenerative processes are allowed to take place according to the natural healing steps.

Provided herein are methods for manufacturing and using engineered tissues. The methods and compositions described herein may be useful for biocomputing, regenerative medicine, non-clinical trials, or cellular agriculture.

Osteoinductive materials and modular bioactive synthetic bone craft (MST) devices are provided. The MST devices comprise the osteoinductive materials for applications in regenerative medicine and treatment of diseases. The osteoinductive material can be characterized by a scaffold filament, a hydrogel layer cross-linked with the scaffold filament, and an osteoinductive growth factor. The hydrogel layer contains the osteoinductive growth factor, a covalent cross-linking polymer, a covalent cross-linking monomer, and a physical cross-linking polymer.

The invention relates to the technical field of bone tissue engineering and regenerative medicine, and discloses a stem cell-loaded bionic bone scaffold material, which comprises a bone scaffold main body, the bone scaffold main body is formed by compounding hydroxyapatite and a biodegradable high-molecular polymer and has a porous structure, the porosity is 70%-90%, the aperture range is 100-500 microns, and pores are communicated with one another; a vascularization induction factor slow release system is arranged in the porous structure of the bone scaffold main body, the stem cell-loaded bionic bone scaffold material does not need to take the autologous bone of a patient, and the problems of donor injury and limited sources are avoided; the composite scaffold is matched with low-immunogenicity stem cells, so that the risks of immunological rejection and disease transmission are avoided; the material has the advantages of good biocompatibility, mechanical matching with human bones, degradability, no secondary operation, promotion of vascular regeneration and stem cell osteogenesis, and realization of bone regeneration integration.

The invention belongs to the technical field of cytobiology and regenerative medicine, and particularly relates to a porcine-derived corneal limbus epithelial stem cell culture method and application, during culture, pretreatment is firstly carried out, then a culture dish is coated, cells are cultured and passaged after centrifugation, dryness identification is carried out through cell immunofluorescence color, and the porcine-derived corneal limbus epithelial stem cells are obtained. The stem cell engineering vector can be applied to damage repair of corneal diseases including corneal limbal stem cell deficiency, during repair, the stem cell engineering vector is compounded with a biological material to form the stem cell engineering vector, the stem cell engineering vector is implanted into a damaged part, the separation and culture method is simple in step, the cell obtaining amount is large, and the stem cell engineering vector is suitable for being separated from eyeballs of a large animal-pig; the corneal limbus epithelial stem cells cultured in vitro can form cell clusters called stem cell islands, the obtained corneal limbus epithelial stem cells have phenotypes of BMI1 + and delta Np63 alpha +, and CK14 + and ABCG < 2 + >, and a new way can be provided for cell transplantation based on experimental research and clinical research.