76 results about "Regenerative medicine" patented technology

The main objective of this invention is to provide a novel manufacturing method capable of efficiently and directly converting or inducing somatic cells into insulin-producing cells. As an example, a method for manufacturing insulin-producing cells is provided, which involves directly differentiating and inducing insulin production from somatic cells. This method includes a step of culturing somatic cells in a serum-free differentiation-induction medium; or a step of culturing somatic cells in a differentiation-induction medium containing 5 μg / mL or more of insulin. According to this invention, insulin-producing cells with high insulin secretion capacity can be efficiently and directly manufactured from somatic cells. The insulin-producing cells obtained by this invention are useful in regenerative medicine and the like.

The application discloses a method for inducing exosomes by adopting starvation combined gradient hypoxia and the exosomes prepared by the method and application, and belongs to the technical field of biological medicine. In view of the problems of low yield and insufficient activity of the existing exosome preparation method, a gradient hypoxia domestication culture strategy is provided. The method comprises sequentially performing stage hypoxia starvation culture on seed cells under a higher oxygen concentration and a lower oxygen concentration. Through the combined gradient induction mode, the yield of exosomes can be significantly improved, which is more than 2 times that of a normal oxygen group and more than 30% that of a single hypoxia group, and the repair promotion and anti-inflammatory activity thereof is enhanced. The exosomes prepared by the application are uniform in particle size, high in purity and activity, the process is stable and controllable, and are suitable for large-scale production, and are suitable for use in the fields of regenerative medicine, drug delivery and clinical treatment.

This invention provides an application of a quantitative partial reprogramming system in the preparation of drugs to reverse skin aging, belonging to the fields of regenerative medicine and medical aesthetics. The quantitative partial reprogramming system includes a nucleic acid delivery system or a small molecule induction system; skin aging includes photoaging-induced skin aging; reversing skin aging includes increasing the thickness of the dermis, increasing the density of type I collagen, and improving skin elasticity. The quantitative partial reprogramming system provided by this invention can fundamentally reverse the cellular aging clock and achieve structural regeneration, and can be applied to the preparation of drugs to reverse skin aging.

The application belongs to the field of regenerative medicine and nano drug delivery system, and particularly relates to a drug-loaded exosome for improving the testosterone secretion capacity of testicular interstitial cells and a preparation method and application thereof. The application links a targeting FATE1 polypeptide to the surface of MSC-derived exosomes by a chemical coupling method, and loads a small molecule compound in the exosomes by electroporation, so as to obtain modified MSC-derived exosomes. The obtained exosomes can obtain the targeting capacity in testicular interstitial cells, and the natural repair capacity of MSC-derived exosomes and the release of small molecule drugs promote the repair of damaged cells, improve the testosterone secretion capacity of testicular interstitial cells, and relieve male erectile dysfunction caused by diseases or aging.

This invention provides a method for rapid extraction of collagen, belonging to the field of regenerative medicine technology. The method includes the following steps: (1) taking animal tissue material as the extraction raw material, crushing it to remove fat and impurities, and obtaining a precipitate; (2) placing the precipitate into a molding mold to obtain a tissue module, freezing and storing the tissue module to obtain a pretreated material; (3) mixing an acidic aqueous solution and pepsin to obtain an extract; placing the pretreated material in the extract in a suspended state, first allowing it to stand and then extracting it in suspension, then stirring, filtering or centrifuging, and retaining the supernatant to obtain a crude extract; (4) adding inorganic salts to the crude extract, allowing it to stand, filtering, collecting the precipitate, washing it, and then transferring the precipitate into a buffer solution to swell it. Filtering the swollen precipitate and dialyzing it to obtain a collagen aqueous solution, followed by post-processing to obtain collagen. The method for extracting collagen provided by this invention can significantly shorten the extraction time.

The application belongs to the technical field of cell culture and organoid, and particularly relates to a culture medium and a culture method for inducing primitive pluripotent stem cells into amnion-like cells. The application provides a culture medium for inducing primitive pluripotent stem cells into amnion-like cells, and through accurate regulation of key signal pathways such as TGF-beta, Wnt and FGF / ERK, efficient and specific directional differentiation of primitive pluripotent stem cells into amnion-like cells can be achieved. The application has the advantages of clear composition and good repeatability, avoids the use of uncertain components such as serum, and provides a reliable tool for stably obtaining high-quality amnion-like cells. The cells produced thereby are an ideal starting point for constructing amnion organoids and isolating amnion mesenchymal stem cells, and lay an important technical foundation for embryonic development research, disease modeling and regenerative medicine applications.

This invention provides a treatment system for promoting dental pulp repair using oral stem cells, belonging to the field of oral treatment technology. The system utilizes a dynamic gene regulation module, an adaptive intelligent scaffold, and a bio-digital fusion interface in synergy. Through the dynamic gene regulation module and the adaptive intelligent scaffold, the efficiency and therapeutic effect of dental pulp regeneration are significantly improved. The dynamic gene regulation module employs a light-controlled CRISPR system to perform real-time gene editing on dental pulp stem cells, dynamically adjusting according to the needs of the regeneration stage. The adaptive intelligent scaffold, based on 3D printing technology and manufactured using a digital model of the patient's teeth, can dynamically release growth factors and optimize the microenvironment. This combination of personalized design and dynamic regulation allows for adaptive adjustments to meet the specific needs of the patient, not only improving the repair efficiency of dental pulp tissue but also enhancing the success rate of functional reconstruction. This provides patients with dental pulp injury with a more efficient and precise treatment option, promoting the personalized development of oral regenerative medicine.

A silicon-anchored hardware enforcement system enables low-latency, cryptographically gated execution of artificial intelligence for stem cell therapy and regenerative medicine. A Sovereign Identity Token derived from a Physical Unclonable Function (PUF) permanently binds model decryption to a specific hardware instance and restricts execution to a Trusted Execution Environment synchronized to a hardware-protected Safety Epoch. Encrypted AI model weights are decryptable only upon successful hardware validation. Multimodal clinical inputs, including genetic, imaging, and structured patient data, are integrated through a hardware-constrained fusion architecture that operates under enforced biological safety parameters. FPGA-implemented predicate logic evaluates defined biological safety conditions at sub-millisecond latency, and an ASIC-based nullification circuit irreversibly suppresses outputs that violate hardware-defined thresholds within a bounded millisecond response time. A distributed revocation protocol propagates credential invalidation across networked nodes within sub-second latency while preserving reduced-capacity safe mode operation. A permissioned provenance ledger records hardware-attested execution events and supports automated regulatory documentation. The system provides secure clinical deployment, federated research enablement, and verifiable auditability for high-risk therapeutic environments.

This invention belongs to the field of biomedicine and regenerative medicine technology. It discloses a chimeric polypeptide, related biomaterials, and their application in inducing cardiomyocytes. The chimeric polypeptide is obtained through family homologous domain substitution and is selected from the following groups: (1) a MEF2 chimeric polypeptide with MEF2C protein as the backbone, wherein the N-terminal and C-terminal transcriptional activation domains are replaced by homologous regions of MEF2B and MEF2D, respectively; (2) a GATA chimeric polypeptide with GATA4 protein as the backbone, wherein the N-terminal transcriptional activation domain is replaced by homologous regions of GATA1 and GATA6. This invention also provides the nucleic acid encoding the polypeptide, the construct, and a method for efficiently inducing fibroblast reprogramming into cardiomyocytes under in vitro conditions. When the chimeric polypeptide of this invention is used alone or in combination, the induced cells exhibit calcium ion oscillations earlier and have higher maturity.

This application discloses a hydrogel composition, its preparation method, and its applications, relating to the fields of regenerative medicine and biomedical materials technology. The hydrogel composition comprises o-nitrobenzyl alcohol-modified tetra-arm polyethylene glycol and methacrylated hyaluronic acid. The hydrogel composition provided in this application addresses the problems in related technologies that rely on tissue flaps or prostheses for repair, such as large trauma, easy foreign body reaction, asymmetrical shape, unnatural feel, and the inability of tissue engineering materials to be rapidly molded intraoperatively, requiring secondary surgery.

本发明公开了一种CD34+造血干细胞化学重编程为诱导多能干细胞的方法。所述方法包括：将CD34+造血干细胞分散为单细胞，接种于包被细胞外基质的培养表面，在无血清培养基中依次进行三阶段诱导培养：第一阶段培养基包含丙戊酸、CHIR99021、RepSox、EPZ6438、PY60、OAC1和OAC2；第二阶段培养基包含丙戊酸、CHIR99021、RepSox、反苯环丙胺、TTNPB、Forskolin、OAC1和OAC2；第三阶段培养基包含CHIR99021、Forskolin、Y‑27632、SP600125、OAC1和OAC2。最后挑取形成的iPSC克隆进行扩增培养，获得诱导多能干细胞。本发明针对CD34+造血干细胞优化了小分子组合和诱导时序，全程无病毒、无转录因子、无血清，重编程效率可达3%以上，细胞凋亡率低，可获得具有完整多能性和正常核型的iPSC，适用于临床级细胞制备和再生医学应用。

This invention belongs to the field of stem cell sorting technology and discloses a method for preparing canine adipose-derived MUSE cells using magnetic beads and its application. The method first involves digesting canine adipose tissue with collagenase and then expanding it by adhesion. Subsequently, FITC-labeled SSEA-3 antibody is combined with anti-FITC magnetic beads, and SSEA-3 positive cells are captured using EasySep™ column-free magnetic bead sorting technology. The sorted positive cells are then seeded into a microsphere culture apparatus containing 81 microwells for induction culture. Experiments show that the purity of the MUSE cells obtained by this method is over 90%, and the spontaneously reconstructed colonies (M-clusters) in the microwell system exhibit strong SSEA-3 fluorescent expression. This invention effectively solves the problems of low initial content of canine MUSE cells and significant damage during flow cytometry sorting, providing a high-quality source of seed cells for canine regenerative medicine treatment.

The objective is to discover novel substances that have higher proliferation-promoting activity for stem cells, and to provide stem cell proliferation promoters, methods for promoting stem cell proliferation, and cosmetics, pharmaceuticals, quasi-drugs, or food and beverages containing stem cell proliferation promoters. [Solution] We have invented a stem cell proliferation promoter containing an extract of cocoa husk using a polar solvent as an active ingredient. That is, the present invention can be used as a method for preparing stem cells and / or as a stem cell proliferation promoter in regenerative medicine and regenerative beauty.

The application relates to the field of tissue engineering and regenerative medicine, and particularly discloses a cell-extracellular matrix complex for hair follicle regeneration and a preparation method thereof. The complex comprises autologous hair follicle stem cells, a human scalp-derived acellular ECM scaffold, time-sequentially added growth factors and an anti-scarring component. In preparation, the autologous hair follicle stem cells are first induced by BMP4 and TGF-beta 1 in cooperation, then inoculated into the human scalp-derived acellular ECM scaffold, and the complex is constructed under dynamic culture conditions, and VEGF is added to promote vascularization. The complex can efficiently form a structure-intact hair papilla-like tissue, has good biocompatibility, low immunogenicity and anti-scarring characteristics, and is significantly superior to a traditional collagen scaffold system. The application provides a new regeneration treatment strategy with clear clinical transformation prospects for scaleable preparation for androgenetic alopecia and other hair follicle deficiency diseases.

The application relates to the field of biomaterials and regenerative medicine, in particular to a preparation method and application of fibronectin and collagen protein-loaded PLGA nanoparticles, wherein the preparation method adopts a complex emulsion solvent evaporation method, a buffer solution containing fibronectin, collagen protein and trehalose is used as an inner water phase, a dichloromethane solution of PLGA is used as an oil phase, and a polyvinyl alcohol and trehalose-containing aqueous solution is used as an outer water phase; and the nanoparticles are prepared through emulsification, high-pressure homogenization, dialysis and freeze-drying. The method has the beneficial effects of stable process and effective protection of protein activity. The obtained nanoparticles have uniform particle sizes (100-200 nm), and can realize synergistic controllable release of double proteins. The product organically combines the physical filling support function of PLGA and the biological repair function of active factors, realizes the integration of "filling-regeneration", can significantly reduce the risk of inflammation, and can be safely applied to soft tissue filling, wrinkle improvement and skin wound repair in the field of medical cosmetology.

The present application relates to the technical field of biomaterials and regenerative medicine, and particularly relates to a preparation method of a whole-fish-scale-based bone repair material, comprising the following steps: S1, fish scale fiber preparation: collected fish scales are washed and subjected to decellularization treatment, and then ground into fibrous form; S2, fish scale collagen adhesive preparation: collagen is extracted from the decellularized fish scales by using at least two different extraction methods, the obtained collagen products are mixed, freeze-dried, and then dissolved in deionized water to prepare collagen adhesives with different concentrations; S3, composite material preparation: the collagen adhesive prepared in step S2 is mixed with the fish scale fibers obtained in step S1, and then extruded into a shape; the whole-fish-scale-based bone tissue engineering material can be prepared by combining fish scale collagen and fish scale fibers, the material is not only safer and more reliable in composition, but also allows accurate regulation of the composition, thereby providing more innovative possibilities for the application of fish scale collagen adhesive.

This method for estimating the therapeutic effect of stem cell transplantation comprises: a step (S11) for acquiring a plurality of cell images in which a stem cell group in culture is imaged at a plurality of predetermined time points within a cell culturing period for stem cell transplantation for regenerative medicine purposes; a step (S12) for identifying stem cell regions respectively in the plurality of cell images; a step (S13) for acquiring, on the basis of the identified stem cell regions, a plurality of types of index values associated with the stem cell group with respect to each of the plurality of cell images; and a step (S14) for estimating, before the transplantation of the stem cell group obtained by the cell culturing, the therapeutic effect of the transplantation of the stem cell group on the basis of the acquired plurality of types of index values. The plurality of types of index values acquired in step (S13) include a plurality of types of index values associated with the morphologies of the stem cells.

The application discloses a multifunctional hydrogel patch, a preparation method and application thereof, and belongs to the field of tissue engineering and regenerative medicine. The biological patch comprises a decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs). The dFCT is obtained by decellularization and freeze-drying treatment of tumor tissue, and retains abundant natural extracellular matrix components. The HNPs are extracted from hair and have excellent active oxygen scavenging capacity and photothermal conversion performance. The hydrogel layer is preferably methacrylated gelatin (GelMA). The patch shows significant cell activity promotion and antibacterial capacity in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch has the advantages of simple preparation process, natural component source, multiple functions such as antibacterial, antioxidant and tissue regeneration promotion, and is suitable for wound repair treatment.

The present application relates to polymers, conjugates, hydrogels, and kits comprising 2- methacryloyloxyethyl phosphorylcholine (MPC) and / or copolymers comprising MPC, and collagen or a collagen-like peptide (CLP). The polymers, conjugates, hydrogels, and kits may be used as fillers and sealants for various applications, for example for treating a wound or tissue injury, such as corneal injury or perforation. Devices and systems for the administration of the polymers, conjugates, and hydrogels are also disclosed.

The application provides a preparation method of pericyte-like mesenchymal stem cell exosome and application thereof in a diabetic wound surface, and relates to the fields of biotechnology, nanomedicine and regenerative medicine. The preparation method of the pericyte-like mesenchymal stem cell exosome comprises the following steps: providing pericyte-like mesenchymal stem cells (P-MSC), and inducing culture of mesenchymal stem cells (MSC) in a culture medium containing an effective concentration of a nitric oxide (NO) donor to obtain the pericyte-like mesenchymal stem cells (P-MSC). The application has the advantages of high targeting and precise repair. The P-MSC-Exo prepared by the application is rich in PDGFR-beta on the surface, so that the P-MSC-Exo can be specifically targeted and enriched at the damaged blood vessel endothelium expressing the ligand PDGF-BB, and precise delivery is achieved. The content can directly repair the mitochondrial damage of endothelial cells, and restore the cell function from the root.

The application discloses a three-dimensional cell scaffold based on a leaf vein of nandina domestica and a preparation method and application thereof, relates to the technical field of biomedical materials and tissue engineering, and discloses the following steps: taking the leaf vein of nandina domestica as a base material, and preparing the scaffold through alkali boiling, sodium periodate selective oxidation and amino-silica / chitosan composite modification. The scaffold retains the three-dimensional structure of the natural leaf vein completely, and significantly improves the cell compatibility of the material. Cell experiments prove that the scaffold has no cytotoxicity, can support the three-dimensional growth of mesenchymal stem cells and PC12 cells and the like with high survival rate, and effectively promotes cell proliferation. The method is simple, green, environmentally-friendly and low in cost, and the obtained scaffold has wide application prospects in the fields of tissue engineering, regenerative medicine and drug screening and the like.

The present application relates to the technical fields of cell bioengineering and regenerative medicine, and particularly relates to a mesenchymal stem cell aseptic engineering production device, which comprises a biological reaction tank, the sidewall of the biological reaction tank is provided with a liquid supplementing hole, the inner top wall of the biological reaction tank is fixedly connected with a fixing seat, the bottom of the fixing seat is provided with a driving assembly for outputting rotary power, the driving assembly comprises a gear ring, the bottom of the fixing seat is fixedly connected with a driving part, the output end of the driving part is coaxially fixedly connected with a first gear, the first gear is engaged with a plurality of second gears, the second gears are all engaged with the gear ring, and the second gears are all rotationally matched with fixing shafts, and the top ends of the fixing shafts are fixedly connected with the fixing seat. The present application drives the filter cage to rotate through the gear ring, drives the guide vanes to rotate through the rotating shaft, reduces the retention and accumulation of the cell carriers at the filter holes through the rotation of the filter cage, so that stable separation and drainage are realized in the long-period perfusion culture process, and the risk of filter unit blockage is reduced.

This invention belongs to the field of tissue engineering and regenerative medicine technology, specifically a multi-layered biomimetic skin structure and its synchronous bio-3D printing preparation method and system, comprising a sequentially combined epidermal layer and dermal layer. This invention highly mimics natural skin through a multi-layered structure. A micropillar array in the dermal layer provides a strong mechanical anchor between the epidermis and dermis, while embedded PLGA microspheres can sustainably release VEGF and EGF for 3-4 weeks, continuously promoting repair. Integrated vascular network channels and nerve guidance structures address the challenges of nutrient delivery and the lack of neural perception function, respectively, filling the functional gaps in traditional biomimetic skin. The synchronous bio-3D printing process enables integrated fabrication of the multi-layered structure. Combined with a four-channel printhead and a multi-physics coupling module, along with a visual positioning and pressure feedback system, printing accuracy and stability are ensured, preventing filament breakage or ink overflow. An intelligent cultivation system simulates the physiological environment, further enhancing skin maturity. This invention is applicable to the repair of skin defects such as burns and chronic ulcers.

The present application belongs to the technical field of regenerative medicine. Provided is a multi-zone microlens for corneal implantation. The microlens has a circular curved surface and comprises two zones, namely, a first correction zone and a plano zone. The microlens preferably comprises three zones, namely, two vision correction zones and one plano zone, wherein the two vision correction zones each have the function of correcting vision, the diopter of the plano zone is 0D, and a through hole is provided in the center of the microlens. The applicant has discovered through extensive experimentation that providing the through hole in the center of the multi-zone microlens for corneal implantation can reduce the occurrence of post-operative corneal clouding and opacity. After the multi-zone microlens for corneal implantation provided in the present application is implanted, the patient will have clear vision for both near and distant objects, achieving full-range vision clarity. Further provided in the present application is an application of the multi-zone microlens for corneal implantation in vision correction.

This invention provides the application of T-5224 in the expansion and / or functional enhancement of human circulating hematopoietic stem / progenitor cells, relating to the fields of stem cell biology and regenerative medicine. It is the first time T-5224 has been applied to the expansion of cHSPCs, opening up a new pathway to improve stem cell culture efficacy by regulating downstream transcriptional hubs of inflammatory / stress signals. Accompanied by the upregulation of HOXB4 gene expression, it significantly improves LIN... ‑ CD45RA ‑ CD34 + CD38 low / ‑ The cell expansion factor, maintaining cell stemness and regeneration capacity, provides a new approach to solving the technical problem of the difficulty in rapidly expanding human circulating hematopoietic stem / progenitor cells in in vitro culture while maintaining cell stemness and regeneration potential.