2816 results about "Cell based" patented technology

The present invention discloses devices and methods of performing high throughput screening of the physiological response of cells to biologically active compounds and methods of combining high-throughput with high-content spatial information at the cellular and subcellular level as well as temporal information about changes in physiological, biochemical and molecular activities. The present invention allows multiple types of cell interactions to be studied simultaneously by combining multicolor luminescence reading, microfluidic delivery, and environmental control of living cells in non-uniform micro-patterned arrays.

Tracking cells are used in a memory system to improve the read process. The tracking cells can provide an indication of the quality of the data and can be used as part of a data recovery operation if there is an error. The tracking cells provide a means to adjust the read parameters to optimum levels in order to reflect the current conditions of the memory system. Additionally, some memory systems that use multi-state memory cells will apply rotation data schemes to minimize wear. The rotation scheme can be encoded in the tracking cells based on the states of multiple tracking cells, which is decoded upon reading.

Tracking cells are used in a memory system to improve the read process. The tracking cells can provide an indication of the quality of the data and can be used as part of a data recovery operation if there is an error. The tracking cells provide a means to adjust the read parameters to optimum levels in order to reflect the current conditions of the memory system. Additionally, some memory systems that use multi-state memory cells will apply rotation data schemes to minimize wear. The rotation scheme can be encoded in the tracking cells based on the states of multiple tracking cells, which is decoded upon reading.

Stem cells, including mammalian, and particularly primate primordial stem cells (pPSCs) such as human embryonic stem cells (hESCs), hold great promise for restoring cell, tissue, and organ function. However, cultivation of stem cells, particularly undifferentiated hESCs, in serum-free, feeder-free, and conditioned-medium-free conditions remains crucial for large-scale, uniform production of pluripotent cells for cell-based therapies, as well as for controlling conditions for efficiently directing their lineage-specific differentiation. This instant invention is based on the discovery of the formulation of minimal essential components necessary for maintaining the long-term growth of pPSCs, particularly undifferentiated hESCs. Basic fibroblast growth factor (bFGF), insulin, ascorbic acid, and laminin were identified to be both sufficient and necessary for maintaining hESCs in a healthy self-renewing undifferentiated state capable of both prolonged propagation and then directed differentiation. Having discerned these minimal molecular requirements, conditions that would permit the substitution of poorly-characterized and unspecified biological additives and substrates were derived and optimized with entirely defined constituents, providing a “biologics”-free (i.e., animal-, feeder-, serum-, and conditioned-medium-free) system for the efficient long-term cultivation of pPSCs, particularly pluripotent hESCs. Such culture systems allow the derivation and large-scale production of stem cells such as pPSCs, particularly pluripotent hESCs, in optimal yet well-defined biologics-free culture conditions from which they can be efficiently directed towards a lineage-specific differentiated fate in vitro, and thus are important, for instance, in connection with clinical applications based on stem cell therapy and in drug discovery processes.

A design method for an LSI includes: generating a delay library for use in a statistical STA, wherein the delay library provides a delay function that expresses a cell delay value as a function of model parameters of a transistor; generating a layout data; and calculating a delay value of a target cell based on the delay library and the layout data. The calculating includes: referring to the layout data to extract a parameter specifying a layout pattern around a target transistor; modulating model parameters of the target transistor such that the characteristics corresponding to the extracted parameter is obtained in a circuit simulation; calculating, by using the delay function, a reference delay value of the target cell; and calculating, by using the delay function and the modulation amount of the model parameter, a delay variation from the reference delay value depending on the modulation amount.

A two-way cable network offering high-speed broadband communications delivered via virtual private networks over a multi-channel shared media system. Bi-directional transmission of packet to ATM cell based communications is established between a head end communication controller and a number of subscriber terminal units, whereby individual cells are prioritized and routed according to a virtual connection. Virtual connections are organized to support multiple virtual private networks in a shared media CATV system. The virtual private network to which a particular STU belongs is user selectable and has the flexibility of handling multi up / downstream channels with different MAC domains. The present invention can also handle non-ATM MAC domains via the same common ATM switch. To overcome the limited number of addresses inherent to common ATM switches, a mapping / remapping function is implemented in the port cards. Furthermore, downstream as well as upstream traffic are filtered at each STU. In one embodiment, information pertaining to downstream traffic is used to implement predictive scheduling in order to improve the timing associated with the request / grant cycle. In another embodiment, a user has the ability to select a quality of service that best suits the needs of the current application. In a further embodiment, the scheduling function is associated with each of the receivers in order to provide improved scalability.

Timing synchronization between base stations of uncoordinated communication networks includes obtaining timing synchronization information from one base station, and adjusting a clock of the other station in response to the synchronization information. The timing synchronization information can be identified from a strongest synchronization signal from nearby uncoordinated base stations. The timing synchronization can accommodate clock offsets and frequency offsets.

This invention provides for a novel means for obtaining human idiologs for any non-human antibody to any target by epitope guided replacement of variable regions using competitive cell-based methods in which the competitor can be either the reference antibody or a ligand that binds to the same epitope on the target as the reference antibody.

Stem cells, including mammalian, and particularly primate primordial stem cells (pPSCs) such as human embryonic stem cells (hESCs), hold great promise for restoring cell, tissue, and organ function. However, cultivation of stem cells, particularly undifferentiated hESCs, in serum-free, feeder-free, and conditioned-medium-free conditions remains crucial for large-scale, uniform production of pluripotent cells for cell-based therapies, as well as for controlling conditions for efficiently directing their lineage-specific differentiation. This instant invention is based on the discovery of the formulation of minimal essential components necessary for maintaining the long-term growth of pPSCs, particularly undifferentiated hESCs. Basic fibroblast growth factor (bFGF), insulin, ascorbic acid, and laminin were identified to be both sufficient and necessary for maintaining hESCs in a healthy self-renewing undifferentiated state capable of both prolonged propagation and then directed differentiation. Having discerned these minimal molecular requirements, conditions that would permit the substitution of poorly-characterized and unspecified biological additives and substrates were derived and optimized with entirely defined constituents, providing a “biologics”-free (i.e., animal-, feeder-, serum-, and conditioned-medium-free) system for the efficient long-term cultivation of pPSCs, particularly pluripotent hESCs. Such culture systems allow the derivation and large-scale production of stem cells such as pPSCs, particularly pluripotent hESCs, in optimal yet well-defined biologics-free culture conditions from which they can be efficiently directed towards a lineage-specific differentiated fate in vitro, and thus are important, for instance, in connection with clinical applications based on stem cell therapy and in drug discovery processes.

The present invention provides a multi-component hybrid electrode for use in an electrochemical super-hybrid energy storage device. The hybrid electrode contains at least a current collector, at least an intercalation electrode active material storing lithium inside interior or bulk thereof, and at least an intercalation-free electrode active material having a specific surface area no less than 100 m2 / g and storing lithium on a surface thereof, wherein the intercalation electrode active material and the intercalation-free electrode active material are in electronic contact with the current collector. The resulting super-hybrid cell exhibits exceptional high power and high energy density, and long-term cycling stability that cannot be achieved with conventional supercapacitors, lithium-ion capacitors, lithium-ion batteries, and lithium metal secondary batteries.

The present invention describes methods and cassettes for cell-based toxin detection and organ localization. The cassettes includes an array containing cells and a matrix of openings or depressions, wherein each region of the substrate enclosed by the opening or depression in the matrix forms a domain individually addressable by microfluidic channels in the device.

A Fibre Channel switch is presented that tracks the congestion status of destination ports in an XOFF mask at each input. A mapping is maintained between virtual channels on an ISL and the destination ports to allow changes in the XOFF mask to trigger a primitive to an upstream port that provides virtual channel flow control. The XOFF mask is also used to avoid sending frames to a congested port. Instead, these frames are stored on a single deferred queue and later processed in a manner designed to maintain frame ordering. A routing system is provided that applies multiple routing rules in parallel to perform line speed routing. The preferred switch fabric is cell based, with techniques used to manage path maintenance for variable length frames and to adapt to varying transmission rates in the system. Finally, the switch allows data and microprocessor communication to share the same crossbar network.

A method and apparatus for controlling inter-cell interference in an evolved Node-B for a cellular communication system with a frequency reuse factor of 1 are provided. The apparatus includes a bitmap generator for receiving scheduling information from evolved Node-Bs of a plurality of neighbor cells, and for generating scheduling information including its cell's bitmap information for the resource allocation using the received neighbor cells' scheduling information, and a scheduler for scheduling the resource allocation for UEs in its cell based on the scheduling information provided from the bitmap generator and power allocation information of the UEs in its cell.

Method and apparatus for designing an integrated circuit. A new layout is generated for at least one standard cell that incorporates an auxiliary pattern on a gate layer to facilitate cell-based optical proximity correction. An original placement solution is modified for a plurality of standard cells to permit incorporation of cells containing auxiliary patterns while improving an objective function of a resulting placement solution for the plurality of standard cells.

A method and an apparatus for scheduling transmissions of a plurality of cells in a wireless communications system including one or more base stations is provided. The method comprises providing a set of virtual channels to enable an intra-cell transmission orthogonal to another transmission within each cell of the plurality of cells and inter-cell transmissions orthogonal to other transmissions across a cluster of cells associated with the one or more base stations. The method further comprises exchanging signaling messages between two or more base stations to coordinate scheduling of the intra-cell transmission with the inter-cell transmissions for the cluster of cells. For optimizing a parameter associated with scheduling of a plurality of users from a cluster of cells in a wireless communication system, an optimal power level for the parameter of each active user may be searched to maximize an indication of system capacity of the wireless communication system. This enables a coordinated jointly scheduling of the users active in the cluster of cells based on the optimal power levels such that the total interference within the cluster may be minimized to maximize the system throughput / capacity.

The present invention comprises compositions and methods for treating a tumor or neoplastic disease in a host, The methods employ conjugates comprising superantigen polypeptides or nucleic acids with other structures that preferentially bind to tumor cells and are capable of inducing apoptosis. Also provided are superantigen-glycolipid conjugates and vesicles that are loaded onto antigen presenting cells to activate both T cells and NKT cells. Cell-based vaccines comprise tumor cells engineered to express a superantigen along with glycolipids products which, when expressed, render the cells capable of eliciting an effective anti-tumor immune response in a mammal into which these cells are introduced. Included among these compositions are tumor cells, hybrid cells of tumor cells and accessory cells, preferably dendritic cells. Also provided are T cells and NKT cells activated by the above compositions that can be administered for adoptive immunotherapy.

Microfluidic devices and methods that use cells such as cancer cells, stem cells, blood cells for preprocessing, sorting for various biodiagnostics or therapeutical applications are described. Microfluidics electrical sensing such as measurement of field potential or current and phenomena such as immiscible fluidics, inertial fluidics are used as the basis for cell and molecular processing (e.g., characterizing, sorting, isolation, processing, amplification) of different particles, chemical compositions or biospecies (e.g., different cells, cells containing different substances, different particles, different biochemical compositions, proteins, enzymes etc.). Specifically this invention discloses a few sorting schemes for stem cells, whole blood and circulating tumor cells and also extracting serum from whole blood. Further medical diagnostics technology utilizing high throughput single cell PCR is described using immiscible fluidics couple with single or multi cells trapping technology.

This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a Group H nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.

In a cell based EUI, existing display container cells nested within a “host” display container cell are automatically shifted and / or downsized, if necessary, to increase available space to facilitate the creation of another display container cell nested within the “host” display container cell, in response to a request to perform the creation. Similar shifting and / or downsizing are performed to facilitate expansion of one of the nested display container cells; and shifting and upsizing are performed to facilitate contraction of one of the nested display container cells. In one embodiment, shifting and / or downsizing / upsizing are performed in view re-sizing priorities of the display container cells and attributes of a host display container cell governing placement and / or alignment of immediately nested display container cells. In one embodiment, an efficient extended boundary method is employed.

A terminal 211 measures pilot signals transmitted in directional (beam) patterns from an adjacent cell and an own cell, and estimates reception qualities with the presence (high) / absence (low) of the interference from the adjacent cell. The terminal 211 requests data rate request values (DRC1, DRC2) corresponding to the reception qualities with the presence / absence of the interference to the base station 201. The base station 201 shares beam direction schedule with an adjacent cell base station, and confirms the presence / absence of interference to the terminal 211 for each slot. In accordance with the presence / absence of the interference, the base station 201 selects a suitable one of the two values of the data rates requested from the terminal 211, and modulates the data and transmits it.

We describe herein a cell-based multiplexing technique called detectable cell barcoding (DCB). In DCB, each individual sample is labeled with a different DCB signature that distinguishes each sample by one or both of detected intensity or type of detection characteristic. The samples are then combined and analyzed for a detectable characteristic of interest (e.g., presence of an analyte). By employing multiple distinct DCB labels at varying concentrations, one can perform multiplex analyses on up to hundreds or thousands (or more) of cell samples in a single reaction tube. DCB reduces reagent consumption by factors of 100-fold or more, significantly reduces data acquisition times and allows for stringent control sample analysis.

This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.

A system and method for providing cell-based statistics for messages related to the same call. The system and method can receive messages into a message coverage area, link the messages with a call with which they are associated, determine a radio link associated with the messages, create a data record if the radio link has been added, and providing the cell-based statistics that are associated with the messages and the message coverage area to the data record.

Stem cells products having the potential to support cells of a soft tissue lineage, and methods of preparation and use of those stem cell products are disclosed. The invention also relates to methods for the use of such stem cells products in the regeneration and repair of soft tissue, and in cell-based therapies for of soft tissue conditions.

The present invention provides embodiments of methods for directing traffic between cells of different sizes. One embodiment of the method includes determining, at a mobile unit, whether to hand off from a source cell to a target cell based on information indicating sizes of coverage areas of the source cell and the target cell.