60 results about "Cell sorter" patented technology

According to the sorting control system of the flow cytometry sorting instrument, liquid drop images are shot through a camera, and liquid drop states are quantitatively evaluated and monitored on the basis of image content information. In order to determine the liquid drop breaking state, a liquid drop image stroboscopic shooting mode is designed to observe the liquid drop state, and the STM32H7 provides a synchronous control signal. When the droplet generation state is stable, the droplet breaking image is close to a stationary state. The stroboscope lamp is controlled to be turned on at different moments in a period, so that different states of liquid drop generation can be observed. The time difference between the detection time and the charging time is quantitatively determined through liquid drop delay control, and accurate delay control over sorting is achieved. And finally, a full-automatic algorithm is adopted to calibrate the liquid drop time delay, the liquid drop time delay is continuously tried to be changed, and when the shooting brightness of the sorted CCD camera under a certain liquid drop time delay is the highest, the time delay is considered to be the current liquid drop time delay, so that full-automatic calibration of the liquid drop time delay is realized.

A system includes a flow cell and a fluidics system under pressure causing sheath and sample biological fluids to flow. The fluidics system includes a gas bubble remover to remove and eliminate gas bubbles in the sheath fluid. The flow cell receives sheath fluid from the fluidics system, wherein the sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives drops of the sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths. A droplet deposition unit system in communication with the deflection chamber receives the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.

The invention discloses a liquid flow sample loading pressure control method of a flow cytometry sorter, which belongs to the field of cell sorting, and is characterized in that a liquid flow sample loading system of the flow cytometry sorter is built, a gas supply gas path is connected with a sample flow path and a sheath liquid flow path, and the sample flow path and the sheath liquid flow path are communicated with a dropping liquid generation flow path; the sample flow path is provided with a first electric proportional valve, the first electric proportional valve is used for adjusting the air pressure in the sample tube, the sheath fluid flow path is provided with a second electric proportional valve, and the second electric proportional valve is used for adjusting the air pressure in the sheath fluid barrel; according to the Bernoulli's theorem in fluid mechanics, as # imgabs0 reflects a pressure difference corresponding to a sample loading flow, an input and output pressure difference is adopted to replace the pressure difference corresponding to the sample loading flow to obtain a theoretical value of a first electric proportional valve, and an incremental digital PID control algorithm is adopted to control to obtain kth and (k-1) th pressure output values of a PID controller; therefore, the control precision of the sample loading flow is improved.

An automatic annotation method is implemented as part of an image activated cell sorter. A user inputs descriptive information about the events the user is trying to purify. Unsupervised clustering is used to group events with similar image features. Once clustering is complete, the automatic annotation algorithm uses the prior information and the features extracted during clustering to predict the identity of the events in each cluster and annotate the events.

The invention discloses a method for enriching and identifying tumor cells with different phenotypes based on double-antibody double-particle-size magnetic beads. Epithelial cell specific cell adhesion molecules and interstitial cell specific waveform proteins are combined with magnetic beads with different particle sizes, so that epithelial, interstitial and mixed specific markers of the same cell in a living cell state can be enriched and identified at one time. The magnetic beads specifically capture target cells with different phenotypes, the captured target cells are separated in a cell sorter under the action of a magnetic field, the phenotypes of the target cells are identified according to different particle sizes of the magnetic beads adhered to the target cells, and on the basis, subsequent analysis of more markers can be further performed. According to the invention, epithelial, mesenchymal and mixed target cells can be simultaneously captured from various body fluids in a living cell state, typing and counting can be rapidly carried out through the particle sizes of the magnetic beads adhered to the surfaces of the cells, the living target cells are analyzed more accurately, and the clinical and scientific research application values are improved.

The present application provides a method for screening cells, the method comprising: a step of preparing a plurality of cells, the plurality of cells labeled with a first barcode nucleic acid and treated with a test target, the first barcode nucleic acid associated with the test target; a step of selecting the plurality of cells based on a cell phenotype using an imaging cytometer; and, a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.

The utility model discloses cell screening treatment equipment, which relates to the technical field of cell screening, and comprises an automatic cell sorter and a sealing cover, a buffer top plate is horizontally erected on the top of the automatic cell sorter, and anti-skid supporting legs are arranged at four opposite angles of the bottom of the automatic cell sorter. The sealing cover is mounted at the left end of the vertical face of the front side of the automatic cell sorter, and side protection plates are mounted on the left side and the right side of the automatic cell sorter. According to the cell screening treatment equipment, the sealing cover made of a transparent material is mounted on one side of the opening of the working bin through the damping hinge, so that the sorting table in the working bin can be prevented from external interference as much as possible in the using and operating process, and the effectiveness of equipment operation is guaranteed, and cell materials are not affected; in addition, the side protection plates and the buffer top plate are mounted on the left side, the right side and the top of the automatic cell sorter respectively, so that a certain degree of structural protection can be provided for the automatic cell sorter, and equipment can be conveniently carried through a handle.

In accordance with one embodiment, a method comprises exciting differing fluorochromes to fluorescence that mark a plurality of moving cells; collecting a broadband light signal having a broadband wavelength range from the fluorescence of the differing fluorochromes; splitting out wavelength ranges in the broadband light signal into smaller wavelength ranges in light signal paths; separately detecting, with differing photo detectors, the light signals in each of the smaller wavelength ranges in the light signal paths; generating digital signals, with analog to digital converters, from the detected light signals in the smaller wavelength ranges in the light signal paths; aligning the digital signals over the broadband wavelength range; and combining the aligned digital signals together into a full spectral response based on the broadband light signal having the broadband wavelength range.

The present invention discloses a flow cell sorter nozzle clogging monitoring and cleaning method, belonging to the field of cell sorting. The flow cell sorter nozzle clogging monitoring and cleaning method of the present invention connects a flow resistance element to a sheath liquid output pipeline to increase the flow resistance of the entire flow path, and analyzes the nozzle clogging state by pressure detection of a flow chamber. To facilitate the evaluation of the flow resistance of each component in the liquid path, a flow meter is connected between the sheath liquid flow path and the droplet generator flow path. The flow path between the sheath liquid tube inlet and the nozzle outlet is subjected to circuit equivalence according to the flow path-circuit equivalence principle, and the flow resistance of each component in the liquid path is calculated. The increase ratio of the nozzle flow resistance at time t is calculated, and the nozzle clogging state and whether automatic cleaning is possible are determined based on the error limit and the increase ratio of the nozzle flow resistance.

In one embodiment, a system includes a flow cell with a conductive electrode and a base having an opening allowing variable charged sheathed sample fluid to flow out along a fluid axis, a droplet deflection unit with a pair of charge plates and a deflection chamber with a pivot door closing over the chamber a hardware triggered camera mounted to the back of the deflection chamber, and an LED array strobe light mounted to the pivotal door opposite the camera. The LED array strobe light generates a backlighting for various droplet streams. Triggering of the camera is synchronized with the generation of the backlighting to periodically capture a brightfield still image of deflected droplets and center droplets in the deflection chamber. The captured images can be analyzed for droplet deflection angles and center line for visual feedback control of the deflected droplets and the center droplets in respective droplet streams.

1. Name of the product in this design: Main body of a droplet microfluidic cell sorter. 2. Application of this design: The main body of the droplet microfluidic cell sorter is used for sorting and screening cells. 3. The key design features of this product are its main shape and the pattern of its main view. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The utility model discloses a cell sorter roll-over stand which comprises a fixed frame, the fixed frame is fixedly or detachably installed on an operation base station, a cell sorter supporting frame and a material receiving groove are arranged on the fixed frame, the fixed frame is of a semi-surrounding structure, and the material receiving groove is arranged on the fixed frame. The cell sorter supporting frame is arranged on the fixing frame and connected with the fixing frame in a hinged mode, the material receiving groove is connected with the fixing frame in a hinged mode, a cell sorter is arranged on the cell sorter supporting frame, and a test tube fixing opening used for installing a test tube is formed in the material receiving groove. The cell sorting device has the advantages that the structure is simple, the operation time of experimenters is saved, the overall working efficiency is improved, and in the use state, the poured liquid is poured into the test tubes to be directly collected, so that the operation is more efficient and convenient.

The invention relates to a cell sorter based on a sacrificial-layer-free laser-induced forward transfer technology, relates to the technical field of cell sorting, and solves the technical problems that fragments are easily generated and target cells are polluted during cell sorting in the prior art. The cell sorter sequentially comprises a femtosecond laser, a beam shaper, a sorting chip and a cell receiver in the light path direction, wherein the femtosecond laser and the beam shaper are respectively and electrically connected with the control system. According to the cell sorter based on the sacrificial-layer-free laser-induced forward transfer technology, target cells sorted based on the sacrificial-layer-free laser-induced forward transfer technology do not contain sacrificial-layer fragment pollution, and no pollution interference is caused to follow-up research and treatment of the obtained target cells.

The utility model relates to the technical field of biological medicine engineering discloses a cell sorter sample filling structure, including the placement platform, the rear end fixed mounting of placement platform top has the support frame, and the fixed mounting of the frame body of installation frame has the installation frame, the top rotation of installation frame is connected with the rotating disc, and the disc body of rotating disc is with the center point as array state fixed mounting has the sample hopper, the table body of placement platform is provided with the rotating component, and the middle position department of placement platform table body is provided with the placement component, the inside of sample hopper is provided with cleaning component, and the bottom fixed mounting of sample hopper has the electromagnetic valve. The utility model can be through many groups of sample hopper and can preinstall different samples, and through rotation can realize seamless link, and also make through the program fast switching sequence, flexible adjustment process, guarantee the continuity of realization, through the lifting of cleaning scraper ring help to remove the residue, prevent the cross -contamination between different samples.

A method for enhancing gating performance of a cell sorter to prepare an enriched specimen for optical tomography cell analysis includes introducing a specimen into a FACS to generate 2D event data; generating a first scatterplot of the 2D data; identifying target objects; constructing a boundary within the first scatterplot to produce a first gate; counting target objects within the first gate; comparing the number of target objects within the first gate to a first predetermined value and adjusting the first gate as necessary. A boundary around a set of target objects is constructed in a second scatterplot to produce a subset second gate and target objects within the second gate are counted and the count compared to a second predetermined value. When a boundary around target objects meets specifications the first and second gates are stored in memory and used to enrich patient specimens.

Disclosed is an integrated biocontainment cell sorter that isolates portions of the cell sorter that can create contamination. Two containment systems are utilized. A main cabinet containment system contains input samples. An aerosol management containment area includes a nozzle chamber with a nozzle and a sort chamber with sort plates and collection media that collect a droplet stream from the nozzle. The main cabinet is maintained at a first low pressure and clean air is recirculated under a positive pressure. The aerosol management containment area is kept at a second low pressure, which is lower than the first pressure, so that contamination does not leak from the aerosol management containment area into the main cabinet containment area. A sliding sash window is located over an access opening in the main cabinet and can be moved to access different portions of the main cabinet without changing the substantially constant first low pressure in the main cabinet.

The invention discloses a cell sorting device for tumor cell detection and a use method of the cell sorting device, and belongs to the technical field of cell sorting. The liquid level in a test tube is monitored in real time through an optical liquid level sensor, and a rotating seat drives the test tube to rotate by 90 degrees through a stand column and a fixed disc; the empty test tubes are transferred to one end of the optical liquid level sensor, uninterrupted liquid receiving work is carried out by adjusting the positions of the test tubes in the isolation frame, the test tubes filled with liquid do not need to be disassembled and assembled frequently, and all the test tubes in the isolation frame are moved out of the flow cytometry sorter by holding the grip and pulling the deflection frame downwards. One or more isolation frames are independently moved out of the flow cytometry sorter, and the rotating seats and the test tubes are all taken out of the isolation frames, so that batch detection work can be conveniently carried out on the test tubes, and meanwhile, the situation that all the test tubes in the base are exposed to pollute liquid in other test tubes is prevented; the influence on cell sorting and subsequent detection work is not easily caused.

The invention provides a single cell sorter and a single cell sorting method for rare cells or viruses, which are applied to the technical field of biomedical instruments. The single cell sorter comprises: a droplet injection pump for inputting a mixed solution to be sorted; the oil phase injection pump is used for inputting an oil phase; the liquid drop sample inlet is respectively connected with the liquid drop injection pump and the sorting channel; the oil sample inlet is respectively connected with the oil phase injection pump and the sorting channel; the sorting channel is respectively connected with the liquid drop sample inlet and the oil sample inlet and is used for conveying each liquid drop in the plurality of liquid drops; the sorting electrode is used for applying voltage to the current liquid drop for dielectric sorting when receiving the target voltage signal, so that the current liquid drop is sorted into the sorting pool as a target liquid drop; the sorting tank is connected with the sorting channel and is used for receiving the target liquid drops; the waste liquid pool is connected with the sorting channel and used for receiving the waste liquid drops which are not subjected to voltage application by the sorted electrodes, and cell sorting with differential functions can be achieved through the cell sorting device.

The present disclosure provides a method for screening cells, the method including a step of preparing a plurality of cells which are tagged with a first barcode nucleic acid associated with a test target and treated with the test target, a step of sorting the plurality of cells based on cellular phenotype using an imaging cell sorter, and a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.

In one embodiment, a system includes a flow cell with a conductive electrode and a base having an opening allowing variable charged sheathed sample fluid to flow out along a fluid axis, a droplet deflection unit with a pair of charge plates and a deflection chamber with a pivot door closing over the chamber a hardware triggered camera mounted to the back of the deflection chamber, and an LED array strobe light mounted to the pivotal door opposite the camera. The LED array strobe light generates a backlighting for various droplet streams. Triggering of the camera is synchronized with the generation of the backlighting to periodically capture a brightfield still image of deflected droplets and center droplets in the deflection chamber. The captured images can be analyzed for droplet deflection angles and center line for visual feedback control of the deflected droplets and the center droplets in respective droplet streams.

1. The name of the design product: cell sorter. 2. The use of the design product: for cell sorting. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view 1.

The invention provides a flow-type separation droplet delay determination method and system and a flow-type cell sorter, and the method comprises the following steps: enabling calibration particles to pass through a detection area and be identified by a laser detector, and generating a trigger signal; acquiring a first induction signal and a second induction signal; obtaining a first time difference based on the trigger signal and the first induction signal; obtaining a second time difference based on the first induction signal and the second induction signal; acquiring an image of a liquid column between the position of the second sensor and a liquid drop breaking point, and determining a period number of a waveform formed by a liquid drop between the second sensor and the liquid drop breaking point based on the image; the total liquid drop delay time is obtained, the total delay is decomposed into the time difference capable of being directly measured with high precision and the waveform period capable of being directly counted, the problems that a traditional trial-and-error method is low in efficiency, depends on experience and is insufficient in precision are solved, and rapid, direct, accurate and automatic calibration of the liquid drop delay time is achieved.

A collecting system with a magnetically coupled sample mover is provided for flow cytometry and cell sorter systems. The collecting system uses magnets in a driver carriage to control the position of other magnets in a follower carriage. The driver carriage can thereby control the position of the follower carriage without physically touching the follower carriage.

The utility model discloses a cell sorter of preventing overheat failure relates to cell sorter technical field, including cell sorting platform, cell sorting platform outer wall one side fixedly connected with a plurality of upper electromagnetic valve, and cell sorting platform top surface one side fixedly connected with a plurality of lower electromagnetic valve simultaneously, cell sorting platform top outer wall fixedly connected with a plurality of liquid bag hooks, and cell sorting platform top surface one side fixedly connected with display screen, cell sorting platform top surface one side fixedly connected with peristaltic pump, the utility model discloses when cell sorting platform continuous screening discernment cell, if temperature rises to need heat dissipation, operating personnel pass through the both sides sliding module of pushing, and the stable movement of adjusting component along with guide slide rail, with the gradual alignment of through groove and rectangular window, after the impurity of outside air through filter screen, through the through groove and enter the inside of instrument, quickly take away the heat of accumulation, reduce the signal fluctuation of sensor and appear the situation of overheat.

The invention discloses a culture method of cornu cervi pantotrichum stem cells, and belongs to the technical field of tissue engineering. The culture method comprises the following steps: taking antler stem periosteum tissue, refrigerating and transporting the antler stem periosteum tissue through a special transportation preserving fluid, cutting, cleaning and centrifuging to obtain tissue precipitate; resuspending the precipitate by using an optimized serum-free culture medium, inoculating to a culture dish pre-coated with gelatin according to a specific density, standing and culturing, and removing non-adherent cells; after trypsinization and centrifugation, cells are resuspended by using a sorting buffer solution, and a cell population of a specific surface marker is screened by using a flow cytometry sorter, so that the antler stem cells are obtained; and finally, carrying out passage amplification under a low-oxygen condition to obtain the pilose antler stem cells after passage amplification. By optimizing a transportation and preservation system, a culture environment and a sorting process, the purity, the activity and the proliferation and differentiation capacity of the pilose antler stem cells are effectively improved, the process standardization degree is high, and reliable technical support is provided for application of the pilose antler stem cells in the fields of tissue engineering, regenerative medicine and the like.

A circular nozzle assembly is disclosed. A cuvette nozzle subsystem (assembly) for flow cytometry systems is disclosed including a cuvette assembly and a circular nozzle assembly selectively engaged with the cuvette assembly. The cuvette assembly includes a cuvette having a pocket and a flow channel, and a receptacle coupled within the pocket to the cuvette. The receptacle has a through-hole with a tapered conical portion and a circular cylindrical portion. The circular nozzle assembly includes an o-ring gasket coupled to a nozzle body with a flow channel. A tapered conical portion of the nozzle body engages the tapered conical portion of the through-hole to align the respective flow channels of the cuvette and the nozzle body together.

This invention discloses a cell sorting device and its usage method for tumor cell detection, belonging to the field of cell sorting technology. An optical liquid level sensor monitors the liquid level in the test tubes in real time. The rotating seat rotates the test tubes 90 degrees via a column and a fixed plate, transferring empty test tubes to one end of the optical liquid level sensor. Continuous liquid collection is achieved by adjusting the positions of multiple test tubes in the isolation rack, eliminating the need for frequent disassembly and reassembly of full-filled test tubes. By holding the handle and pulling down the deflector, all test tubes in the isolation rack are removed from the flow cytometer. By individually removing one or more isolation racks from the flow cytometer, the rotating seat and all test tubes are removed from the isolation racks, facilitating batch testing of the test tubes. This also prevents all test tubes inside the base from being exposed and contaminating the liquid inside other test tubes, minimizing the impact on cell sorting and subsequent testing.

An integrated biological protection cell sorter is disclosed that isolates portions of the cell sorter that may cause contamination and includes two protection systems. The main cabinet protection system contains an input sample. The aerosol management guard includes a nozzle chamber having a nozzle, and a sorting chamber having a sorting plate and a collection medium that collects a stream of droplets from the nozzle. The main cabinet is maintained at a first low pressure, and clean air circulates at a positive pressure. The aerosol management guard zone is maintained at a second low pressure that is lower than the first pressure such that contaminants do not leak from the aerosol management guard zone to the main cabinet guard zone. A sliding sash is located above an access opening in the main cabinet and is movable to enter different portions of the main cabinet without changing a substantially constant first low voltage in the main cabinet.

In one embodiment, a system includes a flow cell assembly having a flow cell body with a base having an opening allowing sheathed sample fluid to flow out, a bracket coupled to the flow cell body, a flat mirror mounted to the bracket, a hardware triggered camera coupled to the bracket on one side, and a diffused light emitting diode strobe light coupled to the bracket on an opposite side. The camera and strobe light are pointed at the mirror on opposite sides. Activation of the diffused LED strobe light generates a diffused strobe light into the mirror backlighting a droplet stream. Triggering of the camera is synchronized with the generation of the strobe light to periodically capture a brightfield still image of the droplet stream. The image can be analyzed for measured jet breakoff point and measured droplet interval point for visual feedback control of the droplet stream.

1. Name of the product in this design: Cell Sorter. 2. Purpose of this design: For sorting cells. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 1.