42 results about "Cell sorter" patented technology

According to the sorting control system of the flow cytometry sorting instrument, liquid drop images are shot through a camera, and liquid drop states are quantitatively evaluated and monitored on the basis of image content information. In order to determine the liquid drop breaking state, a liquid drop image stroboscopic shooting mode is designed to observe the liquid drop state, and the STM32H7 provides a synchronous control signal. When the droplet generation state is stable, the droplet breaking image is close to a stationary state. The stroboscope lamp is controlled to be turned on at different moments in a period, so that different states of liquid drop generation can be observed. The time difference between the detection time and the charging time is quantitatively determined through liquid drop delay control, and accurate delay control over sorting is achieved. And finally, a full-automatic algorithm is adopted to calibrate the liquid drop time delay, the liquid drop time delay is continuously tried to be changed, and when the shooting brightness of the sorted CCD camera under a certain liquid drop time delay is the highest, the time delay is considered to be the current liquid drop time delay, so that full-automatic calibration of the liquid drop time delay is realized.

A system includes a flow cell and a fluidics system under pressure causing sheath and sample biological fluids to flow. The fluidics system includes a gas bubble remover to remove and eliminate gas bubbles in the sheath fluid. The flow cell receives sheath fluid from the fluidics system, wherein the sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives drops of the sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths. A droplet deposition unit system in communication with the deflection chamber receives the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.

An automatic annotation method is implemented as part of an image activated cell sorter. A user inputs descriptive information about the events the user is trying to purify. Unsupervised clustering is used to group events with similar image features. Once clustering is complete, the automatic annotation algorithm uses the prior information and the features extracted during clustering to predict the identity of the events in each cluster and annotate the events.

The present application provides a method for screening cells, the method comprising: a step of preparing a plurality of cells, the plurality of cells labeled with a first barcode nucleic acid and treated with a test target, the first barcode nucleic acid associated with the test target; a step of selecting the plurality of cells based on a cell phenotype using an imaging cytometer; and, a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.

In one embodiment, a system includes a flow cell with a conductive electrode and a base having an opening allowing variable charged sheathed sample fluid to flow out along a fluid axis, a droplet deflection unit with a pair of charge plates and a deflection chamber with a pivot door closing over the chamber a hardware triggered camera mounted to the back of the deflection chamber, and an LED array strobe light mounted to the pivotal door opposite the camera. The LED array strobe light generates a backlighting for various droplet streams. Triggering of the camera is synchronized with the generation of the backlighting to periodically capture a brightfield still image of deflected droplets and center droplets in the deflection chamber. The captured images can be analyzed for droplet deflection angles and center line for visual feedback control of the deflected droplets and the center droplets in respective droplet streams.

1. Name of the product in this design: Main body of a droplet microfluidic cell sorter. 2. Application of this design: The main body of the droplet microfluidic cell sorter is used for sorting and screening cells. 3. The key design features of this product are its main shape and the pattern of its main view. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The utility model discloses a cell sorter roll-over stand which comprises a fixed frame, the fixed frame is fixedly or detachably installed on an operation base station, a cell sorter supporting frame and a material receiving groove are arranged on the fixed frame, the fixed frame is of a semi-surrounding structure, and the material receiving groove is arranged on the fixed frame. The cell sorter supporting frame is arranged on the fixing frame and connected with the fixing frame in a hinged mode, the material receiving groove is connected with the fixing frame in a hinged mode, a cell sorter is arranged on the cell sorter supporting frame, and a test tube fixing opening used for installing a test tube is formed in the material receiving groove. The cell sorting device has the advantages that the structure is simple, the operation time of experimenters is saved, the overall working efficiency is improved, and in the use state, the poured liquid is poured into the test tubes to be directly collected, so that the operation is more efficient and convenient.

The invention relates to a cell sorter based on a sacrificial-layer-free laser-induced forward transfer technology, relates to the technical field of cell sorting, and solves the technical problems that fragments are easily generated and target cells are polluted during cell sorting in the prior art. The cell sorter sequentially comprises a femtosecond laser, a beam shaper, a sorting chip and a cell receiver in the light path direction, wherein the femtosecond laser and the beam shaper are respectively and electrically connected with the control system. According to the cell sorter based on the sacrificial-layer-free laser-induced forward transfer technology, target cells sorted based on the sacrificial-layer-free laser-induced forward transfer technology do not contain sacrificial-layer fragment pollution, and no pollution interference is caused to follow-up research and treatment of the obtained target cells.

The utility model relates to the technical field of biological medicine engineering discloses a cell sorter sample filling structure, including the placement platform, the rear end fixed mounting of placement platform top has the support frame, and the fixed mounting of the frame body of installation frame has the installation frame, the top rotation of installation frame is connected with the rotating disc, and the disc body of rotating disc is with the center point as array state fixed mounting has the sample hopper, the table body of placement platform is provided with the rotating component, and the middle position department of placement platform table body is provided with the placement component, the inside of sample hopper is provided with cleaning component, and the bottom fixed mounting of sample hopper has the electromagnetic valve. The utility model can be through many groups of sample hopper and can preinstall different samples, and through rotation can realize seamless link, and also make through the program fast switching sequence, flexible adjustment process, guarantee the continuity of realization, through the lifting of cleaning scraper ring help to remove the residue, prevent the cross -contamination between different samples.

A method for enhancing gating performance of a cell sorter to prepare an enriched specimen for optical tomography cell analysis includes introducing a specimen into a FACS to generate 2D event data; generating a first scatterplot of the 2D data; identifying target objects; constructing a boundary within the first scatterplot to produce a first gate; counting target objects within the first gate; comparing the number of target objects within the first gate to a first predetermined value and adjusting the first gate as necessary. A boundary around a set of target objects is constructed in a second scatterplot to produce a subset second gate and target objects within the second gate are counted and the count compared to a second predetermined value. When a boundary around target objects meets specifications the first and second gates are stored in memory and used to enrich patient specimens.

The invention discloses a cell sorting device for tumor cell detection and a use method of the cell sorting device, and belongs to the technical field of cell sorting. The liquid level in a test tube is monitored in real time through an optical liquid level sensor, and a rotating seat drives the test tube to rotate by 90 degrees through a stand column and a fixed disc; the empty test tubes are transferred to one end of the optical liquid level sensor, uninterrupted liquid receiving work is carried out by adjusting the positions of the test tubes in the isolation frame, the test tubes filled with liquid do not need to be disassembled and assembled frequently, and all the test tubes in the isolation frame are moved out of the flow cytometry sorter by holding the grip and pulling the deflection frame downwards. One or more isolation frames are independently moved out of the flow cytometry sorter, and the rotating seats and the test tubes are all taken out of the isolation frames, so that batch detection work can be conveniently carried out on the test tubes, and meanwhile, the situation that all the test tubes in the base are exposed to pollute liquid in other test tubes is prevented; the influence on cell sorting and subsequent detection work is not easily caused.

The present disclosure provides a method for screening cells, the method including a step of preparing a plurality of cells which are tagged with a first barcode nucleic acid associated with a test target and treated with the test target, a step of sorting the plurality of cells based on cellular phenotype using an imaging cell sorter, and a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.

In one embodiment, a system includes a flow cell with a conductive electrode and a base having an opening allowing variable charged sheathed sample fluid to flow out along a fluid axis, a droplet deflection unit with a pair of charge plates and a deflection chamber with a pivot door closing over the chamber a hardware triggered camera mounted to the back of the deflection chamber, and an LED array strobe light mounted to the pivotal door opposite the camera. The LED array strobe light generates a backlighting for various droplet streams. Triggering of the camera is synchronized with the generation of the backlighting to periodically capture a brightfield still image of deflected droplets and center droplets in the deflection chamber. The captured images can be analyzed for droplet deflection angles and center line for visual feedback control of the deflected droplets and the center droplets in respective droplet streams.

1. The name of the design product: cell sorter. 2. The use of the design product: for cell sorting. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view 1.

The invention provides a flow-type separation droplet delay determination method and system and a flow-type cell sorter, and the method comprises the following steps: enabling calibration particles to pass through a detection area and be identified by a laser detector, and generating a trigger signal; acquiring a first induction signal and a second induction signal; obtaining a first time difference based on the trigger signal and the first induction signal; obtaining a second time difference based on the first induction signal and the second induction signal; acquiring an image of a liquid column between the position of the second sensor and a liquid drop breaking point, and determining a period number of a waveform formed by a liquid drop between the second sensor and the liquid drop breaking point based on the image; the total liquid drop delay time is obtained, the total delay is decomposed into the time difference capable of being directly measured with high precision and the waveform period capable of being directly counted, the problems that a traditional trial-and-error method is low in efficiency, depends on experience and is insufficient in precision are solved, and rapid, direct, accurate and automatic calibration of the liquid drop delay time is achieved.

A collecting system with a magnetically coupled sample mover is provided for flow cytometry and cell sorter systems. The collecting system uses magnets in a driver carriage to control the position of other magnets in a follower carriage. The driver carriage can thereby control the position of the follower carriage without physically touching the follower carriage.

The utility model discloses a cell sorter of preventing overheat failure relates to cell sorter technical field, including cell sorting platform, cell sorting platform outer wall one side fixedly connected with a plurality of upper electromagnetic valve, and cell sorting platform top surface one side fixedly connected with a plurality of lower electromagnetic valve simultaneously, cell sorting platform top outer wall fixedly connected with a plurality of liquid bag hooks, and cell sorting platform top surface one side fixedly connected with display screen, cell sorting platform top surface one side fixedly connected with peristaltic pump, the utility model discloses when cell sorting platform continuous screening discernment cell, if temperature rises to need heat dissipation, operating personnel pass through the both sides sliding module of pushing, and the stable movement of adjusting component along with guide slide rail, with the gradual alignment of through groove and rectangular window, after the impurity of outside air through filter screen, through the through groove and enter the inside of instrument, quickly take away the heat of accumulation, reduce the signal fluctuation of sensor and appear the situation of overheat.

The invention discloses a culture method of cornu cervi pantotrichum stem cells, and belongs to the technical field of tissue engineering. The culture method comprises the following steps: taking antler stem periosteum tissue, refrigerating and transporting the antler stem periosteum tissue through a special transportation preserving fluid, cutting, cleaning and centrifuging to obtain tissue precipitate; resuspending the precipitate by using an optimized serum-free culture medium, inoculating to a culture dish pre-coated with gelatin according to a specific density, standing and culturing, and removing non-adherent cells; after trypsinization and centrifugation, cells are resuspended by using a sorting buffer solution, and a cell population of a specific surface marker is screened by using a flow cytometry sorter, so that the antler stem cells are obtained; and finally, carrying out passage amplification under a low-oxygen condition to obtain the pilose antler stem cells after passage amplification. By optimizing a transportation and preservation system, a culture environment and a sorting process, the purity, the activity and the proliferation and differentiation capacity of the pilose antler stem cells are effectively improved, the process standardization degree is high, and reliable technical support is provided for application of the pilose antler stem cells in the fields of tissue engineering, regenerative medicine and the like.

A circular nozzle assembly is disclosed. A cuvette nozzle subsystem (assembly) for flow cytometry systems is disclosed including a cuvette assembly and a circular nozzle assembly selectively engaged with the cuvette assembly. The cuvette assembly includes a cuvette having a pocket and a flow channel, and a receptacle coupled within the pocket to the cuvette. The receptacle has a through-hole with a tapered conical portion and a circular cylindrical portion. The circular nozzle assembly includes an o-ring gasket coupled to a nozzle body with a flow channel. A tapered conical portion of the nozzle body engages the tapered conical portion of the through-hole to align the respective flow channels of the cuvette and the nozzle body together.

This invention discloses a cell sorting device and its usage method for tumor cell detection, belonging to the field of cell sorting technology. An optical liquid level sensor monitors the liquid level in the test tubes in real time. The rotating seat rotates the test tubes 90 degrees via a column and a fixed plate, transferring empty test tubes to one end of the optical liquid level sensor. Continuous liquid collection is achieved by adjusting the positions of multiple test tubes in the isolation rack, eliminating the need for frequent disassembly and reassembly of full-filled test tubes. By holding the handle and pulling down the deflector, all test tubes in the isolation rack are removed from the flow cytometer. By individually removing one or more isolation racks from the flow cytometer, the rotating seat and all test tubes are removed from the isolation racks, facilitating batch testing of the test tubes. This also prevents all test tubes inside the base from being exposed and contaminating the liquid inside other test tubes, minimizing the impact on cell sorting and subsequent testing.

An integrated biological protection cell sorter is disclosed that isolates portions of the cell sorter that may cause contamination and includes two protection systems. The main cabinet protection system contains an input sample. The aerosol management guard includes a nozzle chamber having a nozzle, and a sorting chamber having a sorting plate and a collection medium that collects a stream of droplets from the nozzle. The main cabinet is maintained at a first low pressure, and clean air circulates at a positive pressure. The aerosol management guard zone is maintained at a second low pressure that is lower than the first pressure such that contaminants do not leak from the aerosol management guard zone to the main cabinet guard zone. A sliding sash is located above an access opening in the main cabinet and is movable to enter different portions of the main cabinet without changing a substantially constant first low voltage in the main cabinet.

In one embodiment, a system includes a flow cell assembly having a flow cell body with a base having an opening allowing sheathed sample fluid to flow out, a bracket coupled to the flow cell body, a flat mirror mounted to the bracket, a hardware triggered camera coupled to the bracket on one side, and a diffused light emitting diode strobe light coupled to the bracket on an opposite side. The camera and strobe light are pointed at the mirror on opposite sides. Activation of the diffused LED strobe light generates a diffused strobe light into the mirror backlighting a droplet stream. Triggering of the camera is synchronized with the generation of the strobe light to periodically capture a brightfield still image of the droplet stream. The image can be analyzed for measured jet breakoff point and measured droplet interval point for visual feedback control of the droplet stream.

1. Name of the product in this design: Cell Sorter. 2. Purpose of this design: For sorting cells. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 1.

1. The name of the design product: droplet microfluidic cell sorting operation control graphical user interface for electronic equipment. 2. The use of the design product: for displaying program and graphical information of droplet microfluidic cell sorter, realizing human-computer interaction; for displaying on the display panel matched with the droplet microfluidic cell sorter. 3. The design points of the design product: in the front view. 4. The picture or photo that best indicates the design points: front view. 5. The use of the graphical user interface: for the droplet microfluidic cell sorter, for the user to operate on the operation interface. 6. The human-computer interaction mode of the graphical user interface: the front view is the user operation interface; the interface change state diagram is the interface after clicking the video source button in the upper left corner of the user operation interface.

Improved mounts and other elements are provided to facilitate alignment of multi-tube sample holders with multi-stream cell- and / or other particle-sorting apparatus. These elements are able to adjust the location of a tube holder relative to the particle sorting apparatus in several degrees of freedom, allowing the alignment of the tubes to the sorter to be easily and accurately adjusted, thereby improving sorted sample capture and reducing technician effort and. time. Additionally, these elements can include a laser or laser mount to facilitate visualization of the streams relative to the tubes, thereby facilitating adjustment of the relative location of the tubes. Such a laser mount can be adjustable to control the location and orientation of a laser mounted thereto relative to the tubes and / or particle-bearing droplet streams, facilitating visualization of the streams.

The invention provides a flow cell sorter, a method and a sound wave particle control micro-channel chip, and relates to the field of flow sorting, a liquid flow system of the flow cell sorter comprises a sound wave particle spacing adjusting unit, the sound wave particle spacing adjusting unit is configured to generate a sound standing wave field in a sample flow channel, the pitch line or the pitch surface of the sound standing wave field is perpendicular to the liquid flow direction, so that the particles move towards the pitch line or the pitch surface under the action of sound radiation force, and the particles are arranged at preset intervals in the liquid flow direction. According to the invention, under the action of the specially designed micro-channel and the piezoelectric acoustic wave device, the spacing of particles in a sample flow is adjusted, so that the particles are uniformly distributed, the probability that the particles are wrapped by the same liquid drop is reduced, and the sorting yield is improved; the particles are focused to the position of a sound wave nodal line in advance by adopting a sound wave focusing technology before sheath fluid focusing, and the width of the sound wave nodal line is relatively small, so that the consistency of the positions of the sample particles and the consistency of the flow velocity at a high sample flow velocity can be ensured, and the sorting purity and the yield are improved.

The invention relates to the technical field of droplet microfluidics, and discloses a signal synchronous acquisition method, a microfluidic cell sorter and a storage medium wherein the signal synchronous acquisition method comprises the following steps: obtaining a position offset delta X of a fluorescence detection area relative to an image detection area at a preset droplet flow rate, the position offset delta X is related to the image processing time of the image data; adjusting the position of a fluorescence detection area; image data and fluorescence signals of the liquid drops at the preset liquid drop flow speed are synchronously collected. The microfluidic cell sorter comprises a camera, an adjustable excitation light spot module, a PMT and a control unit, wherein the control unit controls starting of the camera and the PMT. A computer program is stored in the storage medium. When a computer program is operated by a processor, the signal synchronous acquisition method is executed. The technical effect of synchronously collecting image data and fluorescence signals is achieved.

An automatic annotation method is implemented as part of an image activated cell sorter. A user inputs descriptive information about the events the user is trying to purify. Unsupervised clustering is used to group events with similar image features. Once clustering is complete, the automatic annotation algorithm uses the prior information and the features extracted during clustering to predict the identity of the events in each cluster and annotate the events.

The invention discloses a method for constructing a 293F constant expression cell line by using lentivirus, and relates to the technical field of biological pharmacy. Comprising the following steps: S1, lentivirus packaging: co-transfecting an expression vector containing a target gene and a selection marker gene and packaging plasmids into packaging cells, culturing, and collecting virus supernatant; s2, cell infection: infecting Expi293F cells which grow in a suspension manner with the virus supernatant obtained in the S1; and S3, flow cytometry sorting: sorting the cells infected in the step S2 by using a flow cytometry sorter to obtain a positive cell population expressing the screening marker gene. According to the invention, a target gene is integrated into a genome of an Expi293F cell, so that a constant expression cell line is successfully constructed; the cell line can stably express the target protein for a long time, the core defect of unstable transient expression is overcome, and a reliable source is provided for application needing to continuously obtain a large amount of recombinant protein.

Disclosed herein are devices, systems, related methods, computing devices, and computer-readable media relating to cell sorting by embedding using a real-time cell sorter. For example, in some embodiments, a method may include receiving first cell sorter data. A first cell sorter data meter may include cell sorter data including microscope data, hyperspectral imaging data, multispectral data, high dimensional vector data, or one or more combinations thereof. In some embodiments, the cell sorter data may include quantitative fluorescence data representative of one or more antibodies bound per cell, antibody binding capacity (ABC), equivalent soluble fluorescent dye molecules (MESF), one or more other fluorescent quantitative indicators, or one or more combinations thereof. In some embodiments, the quantitative fluorescence data comprises one or more fluorescence signals: one or more fluorescent proteins, one or more fluorescent dyes, one or more fluorescently labeled antibodies, or one or more combinations thereof.