86 results about "Cell sorter" patented technology

The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.

A cell sorting chip and a cell sorting technology are to be established which can positively detect and sort a specified cell for cell separation and detection using micro flow paths formed on a substrate, whereby a cell analyzing/sorting device is provided which uses an inexpensive disposable chip replaceable for each sample. To this end, micro flow paths formed on the substrate are formed, and cells are roughly sorted in a first stage and then finely sorted in a second stage. More specifically, cells are sorted roughly by using scattered light or according to intensity of luminescence in the first stage. In the second stage, the roughly sorted cells are sorted with high precision using image recognition.

The invention discloses a cell sorter micro-fluidic chip based on the immunomagnetic separation technology and application thereof in the aspect of enrichment of rare cells, belonging to the technicalfield of micro-fluidic chips. The micro-fluidic chip, improved on the basis of the existing Y-type micro-fluidic chip, comprises a substrate and a cover-slip, wherein a Y-shaped micro-channel, a sample cell (1) and a buffer cell (2) respectively located at two end points of the upper part of the Y-shaped micro-channel, and an effluent cell (3) located at the end point of the lower part of the Y-shaped micro-channel are arranged on the substrate; and an antigen/antibody immunological reaction chamber (4) located on the micro-channel between the cross point (5) of the Y-shaped micro-channel andthe effluent cell (3) is further arranged on the substrate, wherein the width of the antigen/antibody immunological reaction chamber (4) is 2 to 5 times that of the micro-channel. The chip of the invention can effectively and rapidly fix the magnetic beads, increase the sample size of cells, improve the analysis flux, reduce the dead volume of sample introduction and reduce the retention and cross infection.

Methods and apparatus are provided for culturing cells under conditions for determining cellular differentiation and for separating cells from culture media based on differentiation. The apparatus comprises a bioreactor, media reservoir, a magnetic cell separator, an inlet port for adding magnetic particles to the bioreactor, and a circulating pump, wherein the bioreactor, media reservoir, magnetic cell separator and inlet port are on a single fluid circuit. The present method and apparatus provides a method for separating cells from culture without removing the cells from culture, so that un-selected cells may be returned to the bioreactor for further culture. The method employs magnetic labeling in culture, where the magnetic label specifically identifies cells to be distinguished, either by separation or retention in the culture. The method and apparatus are further designed to comprise means for electromechanical stimulation of hum embryonic stem cells for preparation of electrically responsive tissue.

A disposable rapid cell sorter comprises of microfluidic chip with electrodes and sorts biological cells of interest through magnetic field and electric field based on biological cell functional antibody bonded magnetic beads and luminescent labeling.

The present invention provides a cell enrichment/purification device having a function of continuously enriching cells, a function of locating the cells in a particular area of a flow path in a continuous array after the cell enrichment, a function of recognizing the shape of the cells and fluorescence emission from the cells at the same time in units of one cell based on an image, and a function of recognizing the cells based on the information on the shape and fluorescence emission to separate/purify the cells.

The cell-delivery unit of a high-throughput electrophysiological testing system is implemented as a reusable movable unit suitable for repetitive delivery of cells to a disposable, multi-aperture patch-clamp tray. An electric field emanating from the patch aperture is used to align the dispenser with the aperture. A set of electrodes in the nozzle of the dispenser is used to detect the electric field and effect the alignment. According to another aspect of the invention, dielectrophoretic fields produced by sets of electrodes in the nozzle form a retaining cage that is used first to suspend a test cell directly above the patch aperture and then to urge the cell toward it. A movable cell sorter may also be coupled to the cell-delivery unit.

A microfluidic cell sorter has a microfluidic structure (500) with a sample input channel (1 1 0) leading into an observation region (214), two buffer channels (1 1 8) configured to hydrodynamically focus a sample target cell (208) within the observation region, and at least two output channels (1 1 4). Apparatus directs the target cell into a selected output channel based on a cell sorting control signal (61 6). A CARS pulse source (524) generates CARS pulses (526), which are directed to the target cell within the observation region. A detector (530) detects CARS illumination scattered from the target signal and generates a spectrum signal based on the detected illumination. A processor (61 4) identifies the target cell based on the spectrum signal and generates the cell sorting control signal based on the identity of the target cell.

The present invention relates generally to the field of diagnostic and detection assays. More particularly, the present invention provides methods, and reagents including biochips for detecting the presence of, or distinguishing between, one or more analytes in a sample.

The present invention provides HIDE1 as novel monocyte markers. Since HIDE1 are membrane proteins, monocytes can be specifically detected by using antibodies that bind to HIDE1. Further, HIDE1-positive monocytes can also be collected from peripheral blood or the like using a cell sorter, magnet, or such. Monocytes that can be prepared based on the present invention are useful in cell immunotherapy.

A 3-dimensional PDMS cell sorter having multiple passages in a PDMS layer that follow the same path in a DEP separation region and that are in fluid communication with each other within that region. The passages may differ in width transverse to the flow direction within the passages. Flat plates may sandwich the PDMS layer; each plate may have a planar electrode used to generate a DEP field within a sample fluid flowed within the passages. The DEP field may concentrate target cells or particulates within one of the passages within the DEP separation region. The passages may diverge after the DEP-separation region, leaving one passage with a high concentration of target cells or particulates. Techniques for manufacturing such structures, as well as other micro-fluidic structures, are also provided.

Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.

The invention relates to a cell sorter and a cell sorting method. The cell sorter includes a measuring electrode, a working electrode, a detection electrode, and an output section. The measuring electrode forms a measuring electric field in a flow path to measure a complex dielectric constant of each cells flowing through the flow path. The working electrode forms, in the flow path, a working electric field to sort the cells by imparting a dielectrophoretic force to the cells and using the flow path. The detection electrode detects the presence of the cell in the fluid flowing through the flow path. The output section acquires a sorting signal based on information about the measured complex dielectric constant and a detection signal indicating the detection of the cell by the detection electrode. The output section outputs a working signal adapted to form the working electric field to the working electrode when the detection signal is acquired if the sorting signal is acquired.

A method for controlling the speed of releasing the fluorescent probe molecules in the latex emulsion belongs to the material controlled release technical field. The art is as follows: a fluorescent probe molecule water solution or a polymer-fluorescent probe molecule water solution taken as the water phase is added into a mineral oil phase containing a polylol non-ionic surface promoting agent, the volume ratio of the polylol non-ionic surface promoting agent to the mineral oil ranges from 1:19 to 1:10, the solution is evenly stirred at a rotating speed of 8000 to 12000 turns per minute under 273 to 298 K for 1 to 5 minutes to get a water-in-oil latex emulsion, a 0.8 milliliters of water solution containing two to four percent of polyoxyethylene sorbitan laurate is added into to the prepared water-in-oil latex emulsion to be emulsionized for the second time for 2 to 5 minutes to obtain the water-in-oil or oil-in-water latex double emulsion. The sample is analyzed by a fluorescent energetic cell sorter and the release speed of the fluorescent probe molecules is calculated by a formula. The invention has the advantages of high speed, high efficiency, directness and accuracy.

The invention discloses a method for preparing foot-and-mouth disease virus-resistant (FMDV) transgenic livestock by expressing FMDV-resistant ribonucleic acid interference (RNAi), which comprises the following steps of: linearizing an FMDV-resistant RNAi recombinant Lenti-virus vector which carries a short hairpin RNA (shRNA) base sequence and a green fluorescent protein (GFP) reporter gene and transfecting fetus fibroblasts of normal livestock; sieving transgenic nuclear donor cells by using the GFP reporter gene and a flow cell sorter and introducing nuclear donor nuclei into denucleated oocytes of the livestock to obtain a reconstructed embryo; and immigrating the reconstructed embryo into the uterus of surrogate livestock to obtain an offspring, namely the FMDV-resistant RNAi heterozygote transgenic livestock. Heterozygote transgenic male livestock and heterozygote transgenic female livestock are normally mated or reproduced by artificial insemination so as to obtain homozygote transgenic livestock. The transgenic livestock prepared by the method can express the FMDV-resistant RNAi, obviously reduces the FMDV infection rate and has the capability of resisting foot-and-mouth disease (FMD).

The invention discloses an efficient PD-1-CD8+T cell culture method, which specifically comprises: extracting TIL cells; screening the TIL cell suspension by using a flow cell sorter to obtain purified PD-1-CD8+T cells; adding the purified PD-1-CD8+T cells to a G-Rex gas permeable bottle, and culturing for 1 week with a complete culture medium; and rapidly amplifying the PD-1-CD8+T cells. The efficient PD-1-CD8+T cell culture method of the present invention has advantages of effective microbial contamination rate reducing, high amplification efficiency, high cytotoxic activity, and the like.

A cell sorter system that includes a precision pump that causes fluid containing cells to enter an inlet port where the fluid is exposed to source light of one or more wavelengths and where cells scatter light or produce fluorescence by known means, and the scattered light and/or fluorescence is used to detect the presence and position of desired cells. The fluid is passed into a select gate that can be magnetostrictive where it is cause to exit one of a plurality of ports, at least one of these ports receiving the desired cells. A controller controls the precision pump, the detection system, and the select gate so that cells are selected from the fluid. An optional vacuum system can be used to pull fluid or cells out of the selected exit port.

An embodiment relates to a cell sorter that separates fine particles such as specific cells from a sample liquid. The cell sorter separates specific particles in the sample liquid without damage on particles such as cells in the sample liquid. The cell sorter according to the embodiment includes a flow channel (20) which supplies a sample liquid containing particles from one end side; a pluralityof branch channels (21, 22) which are connected to the other end side of the flow channel (20); an image sensor (40) which has a pixel region covering the flow channel (20) and the branch channels (21, 22); a determination unit (50) which determines the characteristics of the particles in the sample liquid from a measurement signal of the pixel region of the portion of the flow channel (20) of theimage sensor (40); and a separation unit (60) which is provided adjacently to the connection of the flow channel (20) and the branch channels (21, 22) and guides the particles in the sample liquid toany of the branch channels (21, 22) based on the determination result of the determination unit (50).

A modular monitor and control system which monitors the fluid jet and droplet formation region in a stream-in-air cell or particle sorter and controls sheath fluid and sample flows via the first and second valves. The system includes a processor which receives digital video image signals of the fluid jet and droplet formation region in the sorter; continuously analyzes the digital image signals to determine a significant change in the digital image signal; and triggers at least a relay switch activation signal in response to a determined significant change. At least one relay switch which is separate from the cell sorter and in communication with the processor is connected with the first and second valves and configured to close the first and second valves upon activation of the relay switch via the relay switch activation signal.