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101 results about "Interstitial cell" patented technology
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Komórki interstycjalne, komórki śródmiąższowe – niewyspecjalizowane, totipotencjalne komórki o charakterze embrionalnym, mogące poruszać się między warstwami oraz przekształcać w inne typy komórek. U jamochłonów występujące głównie między komórkami nabłonkowo-mięśniowymi epidermy, rzadziej między komórkami gastrodermy. Komórki interstycjalne większości parzydełkowców mają formę pojedynczych amebocytów, a u stułbiopławów tworzą zgrupowania.
Schwann cells originating in myeloid interstitial cells
There is provided a method of inducing bone marrow stromal cells to differentiate into bone marrow stromal cell-derived Schwann cells in vitro, comprising the steps of: collecting bone marrow stromal cells from bone marrow and culturing the cells in a standard essential culture medium supplemented with a serum; adding a reducing agent to the culture medium and further culturing the cells; adding a differentiation inducing agent to the culture medium and further culturing the cells; and adding a cyclic AMP-augmenting agent or a cyclic AMP analogue and / or a glial cell differentiation and survival stimulating factor to the culture medium, and further culturing the cells to obtain the bone marrow stromal cell-derived Schwann cells. There are also provided bone marrow stromal cell-derived Schwann cells obtained thereby and a pharmaceutical composition for neural regeneration that comprises them.
Owner:SANBIO
Quercetin crosslinking method for preparing artificial bioprosthesis heart valve materials
The invention discloses a cross-linking preparing method for heart valve prosthesis material, comprising a cross-linker, cross-linking steps and preserving treatment after cross-linking. The method is characterized in that the collected cardiac valve of a pig is timely and appropriately treated and cleaned, and then the cells are completely removed and cleaned, then the obtained cells are cross-linked by quercetin, thus improving the mechanical strength, further eliminating the antigenicity and enhancing the stability. The acellular bioprothesis cardiac valve of the pig adopts heart valve prosthesis prepared by the method has the advantages of non-toxic valve, high tensile strength, resistance to enzymic degradation and minimum release of the cross-linker and self-protein of valves. The cross-linking properties of the acellular bioprothesis cardiac valve made of cardiac valve material of the pig are beneficial to transplanting, proliferation, covering and long-term anti-calcification of interstitial cells of valves of the body of man and vascular endothelial cells after being transplanted into the human body.
Owner:SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
Aculeus type tissue slice glassivation refrigeration carrier and refrigeration method for ovary tissue
ActiveCN101314757AEasy to operatePrevent unstable freezing effectBioreactor/fermenter combinationsBiological substance pretreatmentsNeedle penetrationCell-Extracellular Matrix
An acupuncture-type tissue slice vitrification freezing carrier is composed of a freezing tube with one end being closed and the other end being open, and an acupuncture device. The acupuncture device comprises a needle head and a needle disposed on the needle head and used for threading tissue slices. A section matching with the internal shape and size of the open end of the freezing tube is disposed on the needle head. After the acupuncture device and the freezing tube are combined, the needle is positioned in the freezing tube, and the needle head is adopted as a tube plug of the freezing tube to seal the open end of the freezing tube. A plurality of tissue slices (such as ovary tissue slices and liver tissue slices) can be threaded in a row on the needle at intervals by the carrier so as to be subjected to each operation of the vitrification freezing and storage, and a plurality of sample tissues can be subjected to the vitrification freezing and storage simultaneously, with simple operation and high efficiency, so that the sample tissues can be dehydrated and refrigerated under the same conditions. The storage of the ovary multicellular ingredients (such as the storage of interstitial cells and extracellular matrix ingredients) is superior to the programmed slow freezing and the carrier-free vitrification freezing method.
Owner:THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
Establishment of humanized three-dimensional intestinal mucosa model and application thereof
ActiveCN103255097ALoose connectionReduce expressionMicrobiological testing/measurementArtificial cell constructsCell-Extracellular MatrixECM Protein
The invention provides an establishment of humanized three-dimensional intestinal mucosa model and an application thereof. The model improves a traditional Caco-2 monolayer cell model mainly based on normal physiological structure characteristics of small intestinal epithelium, wherein on basis of adding extracellular matrix, the Caco-2 cells are co-cultured with mucus-secreting cells, interstitial cells and immune cells for a period of time to form a three-dimensional intestinal mucosa model being similar with structure and function of the small intestinal epithelium in vivo. By using the model, a membrane permeating capability at small intestine epithelium as well as a metabolism degree of oral drug molecules can be predicted. The model is mainly used for researching transmembrane transportation and absorption, and metabolism process of medicament molecules at the small intestine in vitro.
Owner:ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
Space cell robot used for truss in-orbit assembly
PendingCN109015614AWith the function of installationLight in massProgramme-controlled manipulatorMetal working apparatusExecution unitEngineering
The invention relates to a space cell robot used for truss in-orbit assembly. The space cell robot consists of interstitial cells, rotating cells, bidirectional interstitial cells, rotating joint cells, claw function cells, a ball head, a passive connecting head, a truss rod, an active connecting head, a groove, and a flange; the interstitial cells and the bidirectional interstitial cells form a connecting unit of the robot, various space cell robot configurations can be formed, the rotating cells and the rotating joint cells form a motion unit of the robot, and the mutual matching of the rotating cells and the rotating joint cells can achieve rotation work of any range in space; and the claw function cells are an end execution unit of the robot, through the synergy matching between the claw function cells and the motion unit, the groove of the active connecting head can be embedded into the flange of the passive connecting head, the assembly work of the truss rod is completed, magnetic automatic locking devices are designed on the active connecting head and the passive connecting head, and the reliability of the connection is ensured.
Owner:HARBIN UNIV OF SCI & TECH
Use of nobiletin in treatment of calcific aortic valvular disease
ActiveCN109464437AReduce calcificationAvoid thickeningOrganic active ingredientsCardiovascular disorderNon surgical treatmentInterstitial cell
The invention relates to the field of prevention and / or treatment of calcific aortic valvular disease. The inventors research and find that nobiletin can effectively down-regulate expression of osteogenic differentiation marker genes BMP2 and RUNX2 in valvular interstitial cells, and inhibit calcification of the valvular interstitial cells, thereby inhibiting or delaying valvular calcification, and further provide use of the nobiletin in preparing medicines for prevention and / or treatment of individual calcific aortic valvular disease. The invention provides a new option for non-surgical treatment of the calcific aortic valvular disease.
Owner:XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
Separation, storage culture and amplification method of pouch fat interstitial cell
InactiveCN102586180APromote wound healingPromote healingDead animal preservationSkeletal/connective tissue cellsLiver functionDiabetic ulcer
The invention relates to a separation and storage method of a pouch fat interstitial cell, which comprises the following steps of: cutting to obtain a pouch fat tissue by adopting a surgical knife, extracting an interstitial tissue of the pouch fat tissue and separating the cell by using an adherent method. The culture and amplification method of the pouch fat interstitial cell comprises the following steps of: carrying out large-scale tissue culture by adopting a paper carrier technology, freezing and storing by using a definite and non-specific genetic component and preserving the cell for a long time. Through the separation and storage method, the pouch interstitial cell can be extracted, massively cultured and preserved for a long time; and the cultured interstitial cell can be used for promoting the healing of skin wound, the healing of diabetic ulcer, the repair of myocardial injury and the repair of hepatic injury.
Owner:EASTERN UNION STEM CELL & GENE ENG
Pericardial heart valve replacement and methods of constructing the same
Owner:STRAKA FRANTISEK +1
Progenitor cells from urine and methods for using the same
Provided herein are urine progenitor cells and methods for producing a culture of urine progenitor cells from a urine sample. The cells may be selected based upon the use of a selective cell medium, based upon morphology, and / or by selecting cell-specific markers. Also provided is an isolated urine progenitor cell that is c-kit positive and can differentiate into urothelium, smooth muscle, endothelium or interstitial cells. Methods of use of urine progenitor cells are provided, wherein cell are seeded onto a tissue scaffold are provided. Methods of treating a subject in need thereof are also provided, including providing a bladder tissue substrate that includes differentiated UPCs and transplanting the substrate into the patient. Finally, kits are provided that include a container suitable for the transport of a urine sample; media; one or more antibiotics; a package for holding said container, media, and antibiotics; and optionally, instructions for use.
Owner:WAKE FOREST UNIV HEALTH SCI INC
Ectonucleotidase pyrophosphate/phosphodiesterase-1 (enpp-1) as a target for the treatment of aortic valve stenosis and cardiovascular calcification
InactiveUS20110098243A1Elevated level of ENPP-High magnitudeBiocideSugar derivativesPhosphodiesteraseEctonucleotidase
Aortic valve stenosis (AS) is a chronic process related to a progressive mineralization of the aortic root and valve cusps. We found in human AS valves a high level of expression and enzymatic activity of ectonucleotide pyrophosphatase / phosphodiesterase-1 (ENPP-1), which correlated to the degree of mineralization. In vitro, inhibition of ENPP activity with ARL 67156 significantly reduced calcification of isolated valve interstitial cells. In a rat model of cardiovascular calcification, ARL 67156 significantly reduced calcification of the aortic root and valve cusps. This is the first study to demonstrate that increased expression and activity of ENPP-1 promotes the mineralization process in AS valves. Hence, inhibition of ectonucleotidase may represent a novel target of therapy for this frequent and serious cardiovascular disease.
Owner:UNIV LAVAL
Culturing method for improving in-vitro differentiation phenotype and function of hepatic cells
InactiveCN105695392ABiocompatibleSupport growthHepatocytesArtificial cell constructsCell-Extracellular MatrixECM Protein
The invention discloses a culturing method for improving the in-vitro differentiation phenotype and function of hepatic cells. The culturing method is characterized by comprising the following steps that the hepatic cells are adopted as seed cells, a porous silk protein scaffold is taken as an in-vitro culturing carrier of the hepatic cells, the hepatic cells, hepatogenic interstitial cells and an extracellular matrix are inoculated to the porous silk protein scaffold according to a certain proportion, and the porous silk protein scaffold is put in a simulated hepatic cell in-vitro culturing environment to be cultured. A three-dimensional hepatic cell co-culturing system built through the method not only can be applied to tissue-engineered hepatic cell function units, but also can be applied to in-vitro evaluation of an influence of drugs on a hepatic metabolism enzyme and hepatic toxicity detection, and therefore a novel screening and evaluating tool is supplied to new drug research and development.
Owner:DALIAN MEDICAL UNIVERSITY
Osteosarcoma circulating tumor cell identification kit
InactiveCN107475356ATyping implementationAvoid false negative resultsMicrobiological testing/measurementLymphatic SpreadInterstitial cell
The invention discloses an osteosarcoma circulating tumor cell identification kit. The kit comprises capture probes for marker gene mRNAs and a signal amplification system. The marker genes comprise at least two epithelial cell marker genes selected from EpCAM, CDH1, KRT8, KRT16, KRT17, KRT18, KRT19 and KRT20 and at least two osteosarcoma metastasis-related interstitial cell marker genes selected from MMP2, MMP9, VIM, CDH2, FN1, HIF-1a, SERPINE1, SURVIVIN, TWIST1, AKT2 and SNAIL. The kit can completely detect osteosarcoma circulating tumor cells, evaluate their metastatic ability to avoid the possibility of the false negative result caused by different expression levels of CTCs in different types of cancers and further improve the accuracy of detection.
Owner:SUREXAM BIO TECH
Method for preparing adriamycin-dipeptide complexes and applications
InactiveCN101274100ALow toxicityHigh selectivityOrganic active ingredientsMacromolecular non-active ingredientsDipeptideReaction rate
The invention discloses a preparation method of an adriamycin-dipeptide compound. Dipeptide or polypeptide containing proline residue is connected to amino of the adriamycin by the acylation through the technique of peptide modification; the toxicity of the adriamycin can be reduced and at the same time the pro-drug can release the adriamycin under the action of tumor interstitial cells so as to ensure that the adriamycin can enrich around tumor tissue to improve the selectivity of the adriamycin to the tumor tissue. The preparation method of the invention has the advantages of fast reaction rate, short term, relatively high productive rate and purity.
Owner:河南省健康伟业生物医药研究股份有限公司
Method for establishing simulated valvular stromal cell calcification model
ActiveCN112725266AImprove stabilityEasy to storeCell dissociation methodsArtificial cell constructsStromal cellInterstitial cell
The invention discloses a method for establishing a simulated valvular stromal cell calcification model, which belongs to the technical field of biomedicine and comprises the following steps: S1, separating primary calcified VICs; S2, preparing a conditional induction culture medium; S3, performing isolated culture on normal VICs; and S4, performing calcification induction. According to the invention, primary-generation VICs of a human or a rat are induced by a collected primary-generation cell-derived conditioned medium of the VICs of a calcified patient, so that the primary-generation VICs of the human or the rat are converted into osteoblast-like cells from the VICs. Therefore, a set of model capable of reflecting the pathological process of a clinical CAVD patient is established, more favorable conditions are provided for researchers, and the model is good in stability, easy to store, simple to prepare, capable of simulating a clinical pathological model to a greater extent and higher in simulation performance.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV
Pericardial heart valve replacement and methods of constructing the same
InactiveUS20150335422A1Minimal exposureControl pressureHeart valvesDead animal preservationCapacitanceCell-Extracellular Matrix
The pericardial heart valve replacement (1) is constructed from living tissue of autologous pericardium containing living pericardial interstitial cells (PICs) and a living extracellular matrix (ECM) in the following way: in the first step the size and shape of the patient's native pathological heart valve is determined using real-time three-dimensional transesophageal echocardiography (TEE) or computer tomography (CT angiography) or magnetic resonance imaging (MRI); in the next step a stented (2) or a stentless pericardial heart valve replacement (1) is constructed from a single sheet of living autologous pericardium obtained from the patient; the size and shape of the pericardial heart valve replacement (1) corresponds to the size and shape of the patient's native heart valve and aortic or pulmonary root. The subject matter of the invention also includes the development of a device (5) for the conditioning and modification of the living autologous pericardial heart valve replacement (1) consisting of a ipulsatile pump (6) driving the flow of culture medium in the device (5) to perfuse the cells in the pericardium, a reservoir (7) regulating the fluid capacitance and function of the pericardial heart valve replacement (1), a chamber (8) containing a holder (9) for the placement of the pericardial heart valve replacement (1) during dynamic conditioning, and a gas exchanger (10a, b) for perfusing C02 into the culture medium. The reservoir (7), the chamber (8) and the gas exchanger (10a, b) are placed in an incubator (11) with an inner atmosphere of air mixed with 5% C02 and a temperature of 36 to 37° C., the individual components of the device (5) being connected by tubing (12).
Owner:STRAKA FRANTISEK +1
Selective cell therapy for the treatment of renal failure
ActiveUS20160002603A1Reduce productionPharmaceutical delivery mechanismBiological particle analysisIn vivoInterstitial cell
Owner:WAKE FOREST UNIV HEALTH SCI INC
Transmembrane anti-inflammatory peptide modified tumor-targeting multi-drug co-loaded liposome and preparation method thereof
PendingCN110548152AGood curative effectSuppresses the inflammatory microenvironmentAntipyreticAnalgesicsTherapeutic effectMedicine
The invention belongs to the technical fields of biomedicine technology and nanomedicine, and discloses a tumor-targeted liposome drug delivery system with three drugs co-loaded. A multifunctional drug delivery system which actively targets tumors is prepared by the steps that cationic liposome is used as a basic carrier, anti-inflammatory drugs and anti-tumor drugs are loaded in the liposome at the same time, a transmembrane anti-inflammatory peptide is covalently linked to the surface of the liposome, and then the outer layer of the cationic liposome is coated with hyaluronic acid. The liposome simultaneously has the functions such as targeted accumulation and deep penetration of tumor tissue, specific recognition of tumor cells and efficient uptake of the tumor cells and interstitial cells thereof. The multifunctional liposome carrier can co-deliver the transmembrane anti-inflammatory peptide, the anti-inflammatory drugs and the anti-tumor drugs to the tumors, fully exerts the synergistic therapeutic effect of the combined drugs, blocks the key signaling pathways of excessive activation of the tumor cells and the interstitial cells, can reduce the level of inflammation and immunosuppression in the tumors, destroy tumor microenvironment, cuts off tumor cell migration pathways, and finally clears the tumor cells and tumor stem cells.
Owner:FUDAN UNIV
Application of XCT790 in preparation of medicine for treating calcified aortic valve diseases
InactiveCN111557937AImproved osteogenic differentiation in vitroOrganic active ingredientsCulture processAntioxidant proteinDisease
The invention relates to application of XCT790 in preparation of a medicine for treating calcified aortic valve diseases and a culture method for inhibiting valve stromal cells from differentiating into osteoblasts. In an osteogenesis-induced in-vitro calcification model, it is shown that XCT790 can inhibit valve interstitial cells from being converted into osteoblasts, and it is proved that the XCT790 plays a role in protection by up-regulating antioxidant protein Hmox1. The beneficial effects of the present invention are mainly reflected in that: application of XCT790 in preparation of the medicine for treating calcified aortic valve diseases is provided, and the method for remarkably improving in-vitro osteogenic differentiation of valve interstitial cells after XCT790 treatment is provided, so that a new medicine choice is provided for calcified aortic valve diseases.
Owner:ZHEJIANG UNIV
Economical, simple and convenient method for inducing lung organs and establishment of experimental model
ActiveCN113151149ASolve hard-to-get problemsReduce the cost of trainingCell dissociation methodsCompound screeningBronchial epitheliumExogenous growth
The invention provides an economic, simple and convenient lung organ culture method and an experimental model, and relates to the technical field of biology. The method comprises the following steps: marking a transgenic mouse with pulmonary epithelial cells by using GFP (Green Fluorescent Protein), sorting the pulmonary epithelial cells and interstitial cells by using a flow cytometry, then co-culturing the sorted lung epithelial cells and mesenchymal cells in an upper-layer small chamber and a lower-layer small chamber divided by Transwell, and promoting the epithelial cells in the upper-layer small chamber to grow and develop into lung organs by using growth factors and biological components secreted by the mesenchymal cells in the lower-layer small chamber. According to the method of the invention, the natural growth environment and mode of in-vivo cells are simulated, and the lung bronchial organ can be obtained under the condition that exogenous growth factors are not added. The mouse lung organ can be used as the experimental model, and can be used for research on lung development process, cell differentiation, lung toxicology, drug screening, organ regeneration and transplantation, etc.
Owner:ANHUI UNIVERSITY
Anti-breeding rodenticide
InactiveCN103125530AReduce in quantityImprove palatabilityBiocideChemosterilantsInterstitial cellBiology
The invention discloses an anti-breeding rodenticide. Melia azedarach oil is served as an anti-breeding agent, and the weight percent of the melia azedarach oil in the anti-breeding rodenticide accouts for 0.08%-0.1%. Through researching the intrinsic rules and hormone regulation mechanism of mouse breeding as well as studying the toxicological and function mechanism of a vegetable anti-breeding agent, ideal anti-breeding agent-melia azedarach oil is screened. The anti-breeding rodenticide is low in effective dosage, makes mice infertile, and has the advantages of low toxicity index, high safety, no harm to human being and animals and no secondary poisoning. After the anti-breeding rodenticide is eaten 10 days later, main reproductive organs-testicles of male mice generate the following variation: the organ coefficient gets small, the sperm amount is reduced, the seminiferous tubule diameter is thinned, lumens are reduced, germinal epithelium thickness is thinned, and interstitial cells are decreased; and ovarial organ coefficient of female mice is obviously reduced, and the corpus luteum in an ovarial structure is contracted. Dead fetus are increased after male and female mice coition, and the breeding number is greatly decreased.
Owner:XIAN UNVERSITY OF ARTS & SCI
CD146 and antibody diagnosis thereof, and application in treating triple negative breast cancer
The invention relates to CD146 and antibody diagnosis thereof, and an application thereof in treating triple negative breast cancers. For a first time, the invention provides that CD146 is a novel target of triple negative breast cancer, and an anti-CD146 antibody might become a novel targeting medicine for treating the disease. Therefore, the invention provides an application of CD146 or anti-CD146 antibody or a functional form of the antibody in preparing medicines used for diagnosing and / or treating triple negative breast cancers. CD146 molecules are subjected to specific high expression in triple negative breast cancer tissues, and induce the occurrences of transformation of epithelial cell to mesenchymal cell in tumor cells. Therefore, tumor invasion migration is promoted. Therefore, the mechanism for CD146 antibody to treat triple negative breast cancer is mainly that the CD146 antibody inhibits the mesenchymal cell characteristics of triple negative breast cancer, and reduces the metastasis invasion capacity thereof. Compared with common chemotherapy medicines, the anti-CD146 antibody has the advantages of low side effects and clear target. The anti-CD146 antibody does not cause whole-body side effect.
Owner:INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
Method for simulating mechanical stimulation of abnormal blood flow to calcification of valvular cells
InactiveCN107488700AShorten the timeHigh simulationMicrobiological testing/measurementElectrical/wave energy microorganism treatmentMagnetic beadCalcification
The invention discloses a method for simulating mechanical stimulation of abnormal blood flow to calcification of valvular cells and relates to the technical field of biological model construction. The method comprises the following steps: S1) adopting a collagenase digestion method for extracting valvular interstitial cells in an aortic valve tissue and preparing into a cell supernatant culture medium; S2) mixing a magnetic bead with an RGD peptide stoste and preparing into a RGD peptide attached magnetic bead; S3) adding the RGD peptide attached magnetic bead into the cell supernatant culture medium acquired in the step S1), abandoning supernatant, cleaning with PBS and then adding into a first culture medium for culturing, putting the first culture medium into a magnetic distortion instrument, simulating the mechanical stimulation of the abnormal blood flow to the valvular interstitial cells by regulating the magnitude, frequency and acting time of the magnetic force of the magnetic distortion instrument and observing the calcification state of the valvular interstitial cells. According to the invention, the micro-simulation for the action of the mechanical stimulation of the abnormal blood flow to the calcification of valvular cells is realized, and meanwhile, the time for constructing a calcification model by a pure calcification culture medium can be shortened.
Owner:XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
Compound stypticum
ActiveCN101138632AReduce dosageGuaranteed curative effectPeptide/protein ingredientsBlood disorderBone Marrow Stromal CellWhite blood cell
A compound medicine for arresting bleeding is characterized in that the compound medicine is a mixture of a batroxobin and an interleukin-11; the clinical dosage of the batroxobin is 0.01-10u and that of IL-11 is 0.06-50mg; the batroxobin is the batroxobin from snake venom of Brazilian spearhead adder, white-eyebrow adder or acutus adder, or the batroxobin from recombinant Brazilian spearhead adder, white-eyebrow adder or acutus adder obtained by genetic engineering; the interleukin-11 is produced by human bone-marrow stromal cells (fibroblasts) and interstitial cells, or the recombinant IL-11 by genetic engineering. The medicine for arresting bleeding can not only ensure the curative effect but also decrease the amount of protease so as to reduce the rate of adverse immune response.
Owner:GRAND LIFE SCI (LIAONING) CO LTD
Extraction analysis and application method of mair-cona active constituents
The invention relates to an extraction analysis and application method of mair-cona active constituents. The extraction analysis and application method of the mair-cona active constituents belongs to the technical field of bioengineering technology and comprises the following steps of (1) preparation of a mair-cona total extract; (2) preparation of five polarity extracts; (3) analysis of the mair-cona extract on DPPH free radical scavenging ability; (4) analysis of the mair-cona extract on testis interstitial cell proliferation and the function in promoting testis to prohibit cells from secreting testosterone; (5) analysis of monoamine oxidase immunocompetence in the mair-cona extract; (6) analysis of the acetylcholin esterase function in the mair-cona extract; (7) analysis of the anti-Glu-SY5Y damage function in the mair-cona extract. According to the extraction analysis and application method of the mair-cona active constituents provided by the invention, active parts of the mair-cona extract are analyzed, the best therapeutic effects of the active constituents on different diseases are obtained, the application of the mair-cona active constituents is promoted, and meanwhile, an effective reference is provided for orientating function development and chemical separation of mair-cona.
Owner:CHANGCHUN UNIV OF CHINESE MEDICINE
Separation and purification and cultivation method of testicular interstitial cells of naked mole rat
ActiveCN106906177ACell state maintenanceHigh purityCell dissociation methodsArtificial cell constructsEnzymatic digestionPurification methods
The invention relates to the field of cell cultivation and in particular relates to a separation and purification method of testicular interstitial cells of a naked mole rat. The interstitial cells of the naked mole rat are separated and purified by adopting enzymatic digestion and a Percoll continuous density gradient method. The method provided by the invention can obtain a lot of testicular interstitial cells of the naked mole rat with normal functional activity simply, efficiently and economically, and cultivation under a low oxygen condition can ensure that the cells still can keep the biological characteristics in an in-vivo state in an in-vitro environment, so that the special physiological functions of the testicular interstitial cells of the naked mole rat can be conveniently and directly researched in a pure in-vitro cell cultivation model further, thereby providing an important theoretical basis for exploring the biological mechanism and applying the same to the clinical related fields.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Interstitial cell for bidirectional connection of spatial cell robots
InactiveCN108890635ACompact structureCapable of bidirectional active connectionProgramme-controlled manipulatorSet screwCam
The invention relates to an interstitial cell for bidirectional connection of spatial cell robots. The interstitial cell for bidirectional connection of spatial cell robots consists of a servo motor,an L-shaped plate, a supporting plate, a transmission shaft, a cam, an incomplete bevel gear, a cross-shaped guide track, a sliding block, a guide track strut, a groove bevel gear, cover plates, a setscrew, connecting surfaces and bosses. The servo motor is a source power device of the interstitial cell, the electrifying phase sequence can be changed, and power for connection and disconnection ofcells is provided; the cam is in contact with the surface of the cross-shaped guide track, the sliding block is connected with the guide track strut to form a guide track assembly, the cam rotates, the cross-shaped guide track can be driven to move forwards, and meanwhile, the guide track assembly is driven to move linearly. The guide track strut is always tangential to a sinusoidal groove in thegroove bevel gear, and by rotation of the groove bevel gear, the guide track assembly can be driven to move in the radial direction. Two connecting mechanisms are fixedly connected through the set screw and the supporting plate to form a hexahedron structure of the interstitial cell. The connecting surfaces are connected with the cover plates through the bosses so as to jointly form a connectingsurface mechanism of the interstitial cell.
Owner:HARBIN UNIV OF SCI & TECH
Method for inducing differentiation of embryonic stem cells into liver tissue structure
ActiveCN105543175ASufficient adhesionGood dispersionGenetically modified cellsCulture processCell adhesionConceptus
The invention belongs to the technical field of cell culture and particularly relates to a method for inducing differentiation of embryonic stem cells into the liver tissue structure. E-cadherin genes serve as exogenous genes to transfect the embryonic stem cells, and high-efficiency expression embryonic stem cells of the E-cadherin genes are obtained; then, culture and differentiation are conducted to obtain an embryoid body, the embryoid body is transplanted into a three-dimensional cell culture system containing an EMT inhibitor for induced culture, and the three-dimensional growing liver tissue structure is obtained. According to the method, ESC in-vitro liver tissue structure differentiation is researched in the perspectives of cell adhesion and the EMT level for the first time, the expression level is stably controlled after E-cadherin transfection, and enough cell adhesion can be kept in cell differentiation, and the cells can not be dispersed into monolayer cells easily. The excessively quick EMT level of the embryonic stem cells in-vitro differentiation is reduced, synchronous differentiation of differentiated parenchymal liver cells and hepatic interstitial cells is controlled to form liver tissue, and the method meets the requirement of an in-vivo tissue development mechanism better.
Owner:THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
Method for producing recombined streptokinase
InactiveCN1796550AThe expression process is simpleHigh expressionEnzymesVector-based foreign material introductionEscherichia coliStreptokinase
This invention describes a process for producing a streptokinase r-SK, comprising the steps of: a) under appropriate expression conditions, cultivating colibacillus engineering cells that carry an expression carrier selected from the following group: the carrier that contains arabinose-induced promoters, or the carrier that not only contains arabinose-induced promoters but also possess the function of directing the targeting proteins to interstitial cells. The coding sequence of the streptokinase is inserted into the expression carrier in order to express soluble and highly active r-SK; b) separating and purifying the step a) wherein said r-SK. In the process of this invention, the expression amount is high, and the separation and purification steps are simple. This invention also describes corresponding expression carriers and engineering cells.
Owner:SHANGHAI NEWSUMMIT BIOPHARMA
In-vitro culture device for highly simulating cancer cell migration
PendingCN109082379AAbility to inhibit metastasisSimple structureTissue/virus culture apparatusCancer cellCell layer
The invention relates to an in-vitro culture device for highly simulating cancer cell migration. The in-vitro culture device comprises a first culture container body, a second culture container body and an osmosis supporting film and is characterized in that the second culture container body is clamped to the first culture container body through an annular retainer block arranged at the top of thesecond culture container body, part of the second culture container body extends into the first culture container body, and the osmosis supporting film is arranged at the bottom end of the second culture container body. The in-vitro culture device has the advantages that the in-vitro culture device is simple in structure and high in practicality, tumor cell nutrition supply, interstitial cells, blood vessels and vascular endothelial cells are considered, the device is close to the growth and transfer environment of in-vivo tumor, tumor cells are transferred into the first culture container body through the vascular endothelial cell so as to simulate the tumor cells entering blood vessels through capillary wall cell layers, and cell migration ability is judged by calculating the number ofthe tumor cells in the first culture container body after culture is performed for a certain period of time.
Owner:WUYI UNIV +1
Application of caffeic acid phenethyl ester in treatment of CAVD
ActiveCN111012772AInhibit calcificationCalcification inhibition or delayOrganic active ingredientsCardiovascular disorderRUNX2Pharmaceutical drug
The invention relates to the field of prevention and / or treatment of CAVD. The inventor finds that caffeic acid phenethyl ester (CAPE) can effectively down-regulate the expression of osteogenic differentiation marker genes RUNX2 and ALP in valvular interstitial cells to inhibit the calcification of the valvular interstitial cells, thereby suppressing or delaying the valve calcification; and thus application of caffeic acid phenethyl ester in preparation of the medicine for preventing and / or treating the individual CAVD is provided. Therefore, novel selection is provided for treating the CAVD by using a non-surgical method.
Owner:XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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