5 results about "Schwann cell" patented technology

The present invention relates to a pharmaceutical composition and cell therapy agent for the prevention or treatment of neurological diseases, comprising Schwann cell precursors (SCPs) or Schwann cells differentiated therefrom, derived from pluripotent stem cells (PSCs) or somatic cells, and natural killer (NK) cells as active ingredients. Specifically, the Schwann cell precursors (SCPs) express at least one selected from the group consisting of GAP43, SOX10, IGFBP2, and combinations thereof; the Schwann cells (SCs) express at least one selected from the group consisting of S100B, SOX10, and combinations thereof; and the natural killer (NK) cells express CD56 + CD16 + It is characterized by expressing at least one selected from the group consisting of the and combinations thereof.

The application discloses application of FTY720 as a PP2A phosphatase activator in preparation of a medicine for treating type I neurofibromatosis. The application first uses FTY720 for treatment of type I neurofibromatosis, and proves that FTY720 significantly inhibits formation of neurofibromas by non-specifically activating PP2A phosphatase. In-vivo experimental results show that FTY720 as a single drug can significantly inhibit tumor growth, and a synergistic effect is presented when FTY720 is combined with a MEK inhibitor, and tumor growth is almost completely inhibited. In-vitro cell experiments show that FTY720 as a single drug or in combination with MEKi treatment can inhibit tumor Schwann cells from forming tumor spheres, inhibit cell proliferation and migration, and induce tumor cell apoptosis. The application overcomes the drug resistance problem existing in the prior art MEK inhibitor, and provides a new treatment strategy for type I neurofibromatosis. FTY720 is an FDA-approved drug, and has good safety and drugability, and has high clinical conversion potential.

This invention discloses a self-generating microneedle nerve conduit that releases NGF in response to ultrasound and its preparation method, comprising: preparing a mixed solution containing a substrate material, a piezoelectric material, and a conductive material; pouring the mixed solution into a microneedle array mold, vacuum drying, and then annealing; removing the treated microneedle patch; immersing the microneedle patch in a mixed solution containing growth factors for half an hour, then removing it and drying it overnight at room temperature; immersing the growth factor-loaded microneedle patch in a coating solution and quickly removing it and placing it in a low-temperature environment overnight; drying the obtained microneedle patch and rolling it into a tubular shape to obtain the desired nerve conduit. The microneedle array structure on the surface of the nerve conduit of this invention can achieve efficient piezoelectricity through the tip effect principle, and can simultaneously achieve electrically controlled release of growth factors, promoting cell proliferation and differentiation, and accelerating the formation of Schwann cell Bunner bands.

The application discloses a kind of recombinant type I humanized collagen and its preparation method and application, belong to genetic engineering field, by bioinformatics method to the core function fragment of human type I collagen alpha 1 chain as shown in SEQ ID NO.1 is analyzed, design and construct the length of 330 amino acids of recombinant type I humanized collagen rCol1, sequence as shown in SEQ ID NO.3;Recombinant type I humanized collagen rCol1 is hydrophilic protein, with good biocompatibility, and with lower antigenicity, can efficiently promote Schwann cell and neuron neurite regeneration;The recombinant type I humanized collagen rCol1 prepared by the method has high purity, no virus hidden danger, has obvious expression abundance;This protein has important application in biomedical, regenerative medicine, tissue engineering, beauty care and cosmetic preparation and the like.

The invention relates to the technical field of biological materials, in particular to a bionic nerve conduit and a preparation method and application thereof. Through layered functional design, the multifunctional nerve conduit which sequentially comprises an inner layer, a middle layer and an outer layer from inside to outside is prepared. Wherein the inner layer is used for constructing parallel oriented nanofibers through an electrostatic spinning technology, and is connected with divalent metal ions after being modified by connecting molecules, so as to regulate and control the behaviors of endothelial cells and blood vigor cells; the middle layer adopts a melt spinning technology to form micron fibers which are arranged in a crossed manner, so that sufficient radial and circumferential mechanical support is provided to enhance the deformation resistance; the outer layer utilizes an electrostatic spinning technology to construct compact and randomly arranged nanofibers, so that on one hand, fibrosis infiltration can be prevented; on the other hand, the structural integrity can be effectively ensured. The bionic nerve conduit provides a new material and a new strategy with clinical transformation potential for peripheral nerve defect repair in a complex environment.