58results about How to "Orderly growth" patented technology

The invention discloses a preparation method of a bionic artificial nerve scaffold established by a collagen. The method comprises the following steps: firstly preparing the collagen in three different shapes for later use: 1) dissolving the frozen and dried collagen by using hexafluoroisopropanol and preparing a nanofiber thin film by using an electrospinning technique; 2) preparing a linear scaffold containing an ordered fine pipeline by the collagen by combining freezing and drying with a mechanical stretching technology; and 3) dissolving the collagen in pure water to prepare a hydrogel; then, coiling the nanofiber thin film into a tube to simulate perilemma, wherein the ordered pipeline linear scaffold is filled in the tube to simulate perineurium; and finally, filling the hydrogel in the pipeline of the linear scaffold and the gap of the scaffold to simulate endoneurium. The bionic artificial nerve scaffold based on a bionic theory better simulates the structure of peripheral nerves and can promote and guide axon and Schwann cells to grow orderly and pass through damaged peripheral nerves based on a good mechanical property.

The invention provides a device for controlling interaction of various cells as well as a manufacturing method and application of the device. The device provided in the invention comprises a glass substrate (1) and a polydimethylsiloxane seal (2) with a plurality of grooves, wherein the polydimethylsiloxane seal is attached to the surface of the glass substrate to respectively form a plurality of micro-channels, the glass substrate in each micro-channel can be selectively modified to be a substrate for promoting cell adhesion by incubating extracellular matrix proteins or can be modified to be a cell adhesion-resistant substrate by assembling polyethylene glycol solution or is not modified. The device is simple and easy to operate, can control fixation, all movement and partial movement of various cells to achieve the purpose of arbitrarily controlling various cells, can be used for researching the interaction among various cells, the pathology or the cell biological development and can also be used for screening medicines.

The invention belongs to the technical field of ecological restoration and particularly relates to a water body ecological restoration method. The water body ecological restoration method comprises the steps that the water surface is cleaned, then submerged plants, emergent aquatic plants and floating-leaved plants are planted, and aquatic animals are put in. According to the water body ecological restoration method, sludge at the water bottom is treated with a plant blanket in-situ coverage method, root systems of the emergent aquatic plants are fixed with pollution-free clay, and a putting-in type planting method is adopted for the floating-leaved plants and the emergent aquatic plants. The aqueous plants can provide an adhesion symbiotic space for microorganisms, and provide a food source for aqueous animals, fish and shellfish are put in when the aquatic plants grow stably, and a rapid and simple water body forest system is formed. By means of the water body ecological restoration method, the effects of fixing sludge at the water bottom, restoring the natural ecological environment and maintaining the water to be clear permanently can be achieved; meanwhile, a dwelling site can be provided for fish and birds, and certain river landscape can be formed.

The invention relates to a device and a method for adhering multiple cells to the same substrate and operating the cells, which divide a gold surface of the substrate into a modifiable region and an unmodifiable region by using a microflow pipeline of PDMS. The method comprises the following steps: introducing different molecular modifications to different pipeline regions to ensure that the substrate is divided into a cell adhesive region and a cell nonadhesive region; inoculating different cells in different adhesive regions, taking away the PDMS, and adhering multiple cells to the surface in sequence; electrochemically desorbing to destroy combination of molecules and gold in the nonadhesive region to ensure that all kinds of cells can move freely; and selectively modifying two sides of one or more cells as the nonadhesive region, separating other cells by using PBS, removing PDMS, and fixing the selected cells to lead other cells to move freely. The method and the device have simple and easy operation, can control fixation, complete motion or partial motion of different cells to achieve the aim of operating multiple cells at will, and can be used for studying interaction among multiple cells and medicinal screening.

The invention relates to a device and an absorption method for adhering a plurality of types of cells on a set position of a same substrate, the device comprises: the substrate with the upper surface which is evaporated with a titanium/chromium adhesion layer and a metal layer and a polydimethylsiloxane seal with a hydrophilic surface and the lower surface which comprises at least one group of micro-groove units; the lower surface of the seal is adhered on the metal layer to form a sealed pipeline by reversible combination; FN is introduced to the second and the fifth grooves to form extracellular matrix proteins within 10min; ethanol water solution of block copolymer of poly (L-lysine)-polyethylene glycol-poly (L-lysine) is introduced to the first and the third grooves to form absorption layers; EG6 is introduced to the fourth and the sixth grooves to form inert mono-molecular film layers in and around the fourth and the sixth grooves. The device and the absorption method can lead a plurality of types of cells to be adjustably and orderly adhered on the same substrate, thereby understanding the effects of a drug to the different types of the cells, screening the drugs and providing a new route for founding the drugs and carrying out the analysis of toxic substances.

The invention discloses a preparation method for a flocculus-shaped SnS<2> negative electrode material of a lithium ion battery. The preparation method comprises the steps of 1) dissolving sodium thiosulfate into deionized water to prepare a solution A, and dissolving stannic chloride pentahydrate into deionized water to prepare a solution B; 2) adding the solution B into the solution A dropwise, continuously stirring to obtain a uniform mixed solution C, and gradually adding ethylene glycol into the mixed solution C to form a uniformly-mixed solution D; 3) adjusting the pH of the mixed solution D to be 2-9 to form a solution E; 4) putting the solution E into a homogeneous hydrothermal reaction kettle to be sealed, and then putting into a homogeneous hydrothermal reactor to be reacted; and 5) after the reaction is completed, taking out a precursor, and performing centrifuging and washing by deionized water and absolute ethyl alcohol separately, and then performing freezing and drying to obtain the flocculus-shaped SnS<2> negative electrode material of the lithium ion battery. The preparation method is low in cost, simple in operation and short in preparation period; and the prepared flocculus-shaped SnS<2> negative electrode material of the lithium ion battery has relatively high cycling stability at high-current density.

The invention belongs to the technical field of biomedicines, and particularly relates to a scar silicone gel. The scar silicone gel is prepared from silicone, bioactive mineral materials, a thickenerand an antioxidant. According to the scar silicone gel, the shortcomings of single structural components and limited effects of existing silicone gel products are overcome. The invention further relates to a preparation method of the scar silicone gel. The preparation method includes the steps that the silicone is weighed according to the formula, and after uniformly dispersing is carried out, the formula mass parts of the bioactive mineral materials, the thickener and the antioxidant are sequentially added, fully and evenly dispersing is carried out, and the obtained mixture is filled in a packaging container to obtain the scar silicone gel. The preparation method is simple in process and convenient for industrialization.

The invention belongs to the field of medical dressings, and particularly relates to a liquid wound spray dressing and a preparation method thereof. The liquid wound spray dressing comprises raw material components of silver sulfadiazine, lidocaine, chitosan, polyvinyl alcohol, ethanol, a preservative, purified water and the like. By optimal design of the component types, proportion and preparation method, long-term bacteriostasis and hemostasis of the liquid wound dressing can be improved, invasion of bacteria can be resisted for a long time, orderly growth and texture repair of wound tissuecan be prompted, and the wound healing process can be accelerated. The liquid wound dressing has definite curative effect, is safe and reliable to use and non-irritant to skin, and has better practical value and popularization and application significance, and the preparation method is quick and simple.

The invention provides a micro-fluidic chip used for reconstructing the bone cell microenvironment and a use method thereof. The micro-fluidic chip is characterized in that a two-dimensional grid pattern with gold as a substrate is constructed, the surface of the pattern is subjected to hydrophobic modification through soaking sulfur alcohol, and therefore cells orderly grow along the pattern; meanwhile, bone cell bodies are covered with calcium alginate polymers, and cell synapsis is exposed. In this way, fluid stress is amplified at the synapsis portions, the problem that in a traditional bone cell fluid stress stimulating experiment, same fluid stress is formed on the surfaces of the cell bodies and the surfaces of the cell synapsis is solved, and the biological characteristics of the bone cells in the physiological status can be more truly reflected.

The invention relates to a method for regulating and controlling the crystal face orientation of a metal material, the metal material obtained through the method and application, and the method comprises the following two steps that the crystal face orientation of the metal material is regulated and controlled through introduction of residual stress and low-temperature annealing, preferred even single crystal face orientation is obtained, and the crystal face orientation of the metal material is regulated and controlled. The method is used for regulating and controlling the electrochemical electron-ion exchange process of a metal material to obtain a high-activity and high-uniformity metal interface, and is successfully applied to the fields of battery metal negative electrode materials, metal negative electrode current collector materials and electro-catalysis.

An autogenous wound tissue regeneration and repairing ointment is characterized by being composed of the following raw materials (by weight): 10-40 parts of a regenerative medical material, 5-20 partsof polyethylene glycol 4000, 30-80 parts of polyethylene glycol 400, 5-25 parts of glycerin, and 0.5-3 parts of medical grade vitamin E oil. By mixing the regenerative medical material, polyethyleneglycol, glycerin and medical grade vitamin E oil according to a certain ratio in a specific way, the effect of guiding ordered creeping substitution of cells, extracellular matrix, blood vessels andother tissues can be achieved, acceleration of wound healing can be promoted, the repair of the nervous system of the wound is synchronized, the orderly growth of the new tissue fiber layer is realized, the regeneration of capillaries and hair follicles is achieved, and autogenous wound tissue regeneration and repairing are realized, and the formation of scars is inhibited. Meanwhile, the PH valueof the mixture is 7.3 to 7.8, which is closer to the pH value of the human body and is less irritating to the human body during use, thereby reducing the pain of patients.

The invention provides a hydrophobic spherulite, a hydrophobic material, a hydrophobic composite material, a Janus composite material as well as preparation methods and application thereof. The hydrophobic spherulite is a hydrophobic spherulite of a micro-nano structure formed by crystallizing a hydrophobic substance, and the micro-nano structure comprises a micron-sized crystal nucleus and a nano-sized crystal whisker growing on the surface of the crystal nucleus, wherein the hydrophobic spherulite is of the micro-nano structure formed by the hydrophobic substance, has a relatively large specific surface area, can load more hydrophobic groups, and has better hydrophobic performance and proper filtering efficiency; according to the hydrophobic material, the hydrophobic spherulite is endowed with multifunctionality by coating the hydrophobic spherulite with a drug layer; and according to the Janus composite material containing the hydrophobic material, a hydrophilic layer is arranged on the surface of one side of the hydrophobic composite material, so that the Janus hydrophilic layer is convenient for water vapor adsorption, the hydrophobic layer improves the concentration gradient of the water vapor, and directional conveying of the water vapor is realized.

The invention relates to the technical field of tissue culture seedling planting, and discloses a strip-shaped tissue culture seedling inoculation device. The strip-shaped tissue culture seedling inoculation device comprises a workbench, a visual detection unit, a stem segmentation unit, a to-be-grafted seedling pickup unit and an inoculation unit, wherein the workbench is provided with a cuttingarea and an inoculation area; the visual detection unit and the stem segment segmentation unit are both mounted in the cutting area of the workbench; the stem segmentation unit is located below the visual detection unit, and the to-be-grafted seedling pickup unit comprises a first manipulator and a tissue culture seedling pickup mechanism; the first mechanical arm is mounted on the workbench, thetissue culture seedling pickup mechanism is mounted on the first mechanical arm, and the inoculation unit comprises second mechanical arms and inoculation handles; and the second mechanical arms are mounted on the workbench, and each inoculation handle is mounted on the corresponding second mechanical arm. The strip-shaped tissue culture seedling inoculation device has the beneficial effects thatthe inoculation work of strip-shaped tissue culture seedlings can be automatically completed, the labor intensity of workers is reduced, the number of operators is reduced, and the production cost issaved for enterprises.

The invention discloses a string bean plantation method, and belongs to the field of agricultural plantation. The string bean plantation method comprise following steps: seed pretreatment, sowing seedling growing, soil preparation fertilization and seedling transplanting, field management, and harvesting. The string bean plantation method is different from conventional trellis building method, trellis is prepared from plastic rods or metal rods instead of bamboo poles or wood rods, can be recycled, and can be inserted into soil directly every year in using, and complex trellis building processis avoided; a triangular pyramid structure is formed, so that trellis stability is increased; short rods are adopted, so that ordered growth of string bean vines is realized, it is convenient for string bean harvesting, string bean harvesting efficiency is increased, and string bean disease resistance and insect resistance are improved.

The invention relates to a method for constructing three-dimensional engineered nervous tissue, the three-dimensional engineered nervous tissue and an application thereof. Process. The method comprises the following steps: 1, carrying out isolated culture on animal neural stem cells; 2, applying a physiological electric field; subculturing the neural stem cells isolated and cultured in the step 1to the fifth generation, centrifuging neural spheres with a diameter of 100-200 microns for later use, pre-melting matrix gel Matrigel on ice for later use, and continuously applying a physiological electric field to stimulate for 7-14 days through physiological electric field application equipment for 1-2 hours every day; 3, optimizing a culture solution suitable for engineering nerve tissue growth: adding 1/50 of B27, 1/100 of N2, 10-20 ng/ml of bFGF and 1% of fetal calf serum into DMEM/F12; and 4, applying the physiological electric field for 7-14 days to obtain the three-dimensional engineered nervous tissue. The technical problems that in the application process of neural tissue engineering, the number of neuronal differentiation of existing neural stem cells is extremely small, and neurite cannot be induced to grow orderly in a three-dimensional growth environment to form a developed neural network are solved.

The invention discloses a preparation method of a full-concentration gradient positive electrode material precursor, a full-concentration gradient positive electrode material and a preparation method of the full-concentration gradient positive electrode material. The method comprises the following steps: (1) adding a nickel-poor solution into a nickel-rich solution to obtain a metal salt solution, adding the metal salt solution, a precipitator a and a complexing agent a into a base solution containing the precipitator and the complexing agent at the same time, and controlling the pH in a reaction system and the concentration of the complexing agent to be gradually reduced along with the reduction of the nickel concentration in the added metal salt solution; and (2) separating, washing and drying the product obtained in the step (1) to prepare the full-concentration gradient positive electrode material precursor with excellent crystallinity. And then preparing the full-concentration gradient positive electrode material with a lithium source. The positive electrode material inherits the excellent crystallinity of the precursor, has high nickel content, high specific discharge capacity and stable structure, is not easy to generate microcracks in the charge-discharge process, and avoids additional side reaction, so that the positive electrode material also has excellent cycle performance.