72 results about "Extracellular ground substance" patented technology

The invention relates to the field of biomedical application of inorganic materials, in particular to application of lanthanum carbonate in preparation of drugs for resisting liver cirrhosis and inhibiting liver inflammations, the drugs comprise lanthanum carbonate and pharmaceutically acceptable auxiliary materials, the drugs are ground and then mixed with food to prepare a drug mixture, and the drug mixture is prepared into the drug for resisting liver cirrhosis and inhibiting liver inflammations. The mass ratio of the lanthanum carbonate in the medicine mixture is 1-10%, and the mechanism of the lanthanum carbonate for treating the liver cirrhosis is as follows: interference or blocking of activation and proliferation of hepatic stellate cells, inhibition of extracellular matrix generation and promotion of extracellular matrix degradation. According to the invention, a rat liver cirrhosis model is constructed through chemical induction, and the effects of reversing liver cirrhosis and inhibiting liver inflammation are found when a rat is treated by taking lanthanum carbonate orally, so that a new candidate drug is provided for clinical treatment of liver cirrhosis.

The invention relates to the technical field of medicines, in particular to application of alpha-ketoglutaric acid in preparation of a medicine for preventing and / or treating myopia. The invention provides application of alpha-ketoglutaric acid or pharmaceutically acceptable salt thereof in preparation of products for preventing and / or treating myopia, and the alpha-ketoglutaric acid is taken as an important intermediate metabolite of tricarboxylic acid cycle in mitochondrial aerobic respiration and has oral safety; researches show that alpha-ketoglutaric acid can inhibit myopia progress and ocular axis elongation by improving sclera fibroblast phenotype transformation and extracellular matrix remodeling, and a new idea is provided for preparation of myopia intervention drugs.

An oral care composition with retinoid compound, amino acid, and / or olive oil. An oral care composition with retinoid compound, a pentapeptide, such as palmitoyl KTTKS [SEQ ID NO. 2], and / or an olive oil. Methods of preventing gingival recession, stopping gingival recession, reversing gingival recession, decrease gum recession, increase gingival barrier protection, promoting collagen synthesis, promoting extracellular matrix synthesis, increasing gum resilience, increasing epidermal thickness and / or promoting fibrillin synthesis, in an oral cavity of an animal by contacting the oral care compositions with at least one surface of the oral cavity.

The invention relates to the technical field of biomedical material modification, in particular to a sulfhydrylation biocompatible polymer derivative, a preparation method thereof, extracellular matrix imitating injectable in-situ cross-linked hydrogel and application thereof. According to the invention, a pre-acylation strategy is firstly carried out on the biocompatible polymer containing both amino and carboxyl, so that a three-dimensional cross-linked byproduct formed by coupling of self amido bonds is avoided, meanwhile, the number of side chain carboxyl for sulfhydrylation modification is increased, and the accurate and adjustable pre-acylation rate can optimize the physical and chemical properties of the derivative; meanwhile, a disulfide bond is used as a protective precursor, active sulfydryl is introduced through amido bond coupling, self-oxidation of the active sulfydryl in the reaction process is avoided, sulfydryl activity is guaranteed, hydrazine-containing reagents are avoided, safety is further improved, virus residues and immunological rejection risks are avoided, universality is high, and the application prospect is wide; and in combination with a high-concentration preparation method, the method has the advantage of large-scale production, and further widens the industrial application potential.

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The invention relates to the technical field of medical nano-materials, and discloses an iron-manganese bimetallic MOFs nano-enzyme hydrogel compound and a preparation method and application thereof, and the method comprises the following steps: step 1, preparing FeMn bimetallic MOFs nano-enzyme MIL-101 by adopting a microwave-assisted solvothermal method; step 2, preparing oxidized hyaluronic acid; step 3, synthesizing a hydrogel precursor protocatechuic aldehyde and a complex PA-Fe of iron ions; and step 4, fully mixing the quaternary ammonium salt chitosan solution with the PA-Fe solution, adding the oxidized hyaluronic acid solution and the MIL-101 powder under continuous magnetic stirring, and finally forming the iron-manganese bimetal MOFs nano-enzyme hydrogel compound. The delivery efficiency of the bimetallic nano-enzyme is improved, the in-vivo retention time of the bimetallic nano-enzyme is prolonged, the bimetallic nano-enzyme has antibacterial, hemostatic, adhesion and anti-oxidation effects, the steady state of an extracellular microenvironment of the intervertebral disc is recovered, an extracellular matrix of intervertebral disc tissue is remodeled, and the effect of promoting the repair and regeneration of the intervertebral disc by the nano-enzyme is synergistically enhanced.

The application discloses a kind of high adhesion enzymatic hydrogel for promoting vascular intimal repair and a preparation method thereof, and belongs to the technical field of hydrogel preparation.The preparation method is as follows: S1, using methacrylic anhydride to modify the double bond of hyaluronic acid, then crosslinking with dopamine to obtain dopamine-modified double-bonded hyaluronic acid;S2, after removing oxygen from the hyaluronic acid solution, add a crosslinking agent and tyramine hydrochloride, and react in an acidic environment to obtain tyramine-modified hyaluronic acid;S3, mix the dopamine-modified double-bonded hyaluronic acid, tyramine-modified hyaluronic acid and deionized water to obtain a hydrogel precursor solution;S4, then use enzymatic reaction to obtain the hydrogel.The method designed by the application is simple, does not require additional light curing equipment, and the constructed hydrogel is similar to the extracellular matrix.The hydrogel can be coated into different shapes to adapt to different environments.The hydrogel is formed by enzymatic reaction, which is rapid, well adhered to the tissue, biocompatible, and suitable for application in vascular repair.

The invention relates to the technical field of biomedical materials and tissue engineering, and discloses a preparation method and application of adjustable porous fat foam. Comprising the following steps: fat tissue pretreatment and freeze-thaw circulation, multi-step combined decellularization and impurity removal, graded washing and purification, freeze grinding and freeze drying, preparation of a decellularized fat matrix suspension, porous foam scaffold forming and foam scaffold gradient rehydration. By adopting a multi-step combined decellularization and impurity removal process, residual lipid droplets in adipose tissues can be actively extruded out by mechanical squeezing assisted by isopropanol degreasing, a lipid wrapping phenomenon is broken, meanwhile, residual nucleic acid and adipose components can be thoroughly removed by virtue of a synergistic degradation effect of nuclease and lipase, the immunogenicity risk is reduced, and the immunogenicity of the adipose tissues is improved. All the steps have a synergistic effect under strict control of temperature, concentration and time parameters, so that the structural integrity of the extracellular matrix is ensured, the decellularization efficiency and thoroughness are improved, and the biological safety and biocompatibility of a final product are ensured.

Methods are disclosed for dissecting a mucosa and a submucosa from a muscularis propria from a region of an organ of a subject, wherein the organ is not the esophagus. In some embodiments, the organ is in the gastrointestinal tract. These methods include injecting submucosally into the organ of the subject a pharmaceutical composition comprising an extracellular matrix (ECM) hydrogel to form a cushion between the submucosa and the underlying muscularis propria at the region of the organ, wherein the ECM hydrogel has the following characteristics: a) a time to 50% gelation of less than 30 minutes at a temperature of about 37° C.; b) a flow viscosity suitable for infusion into the organ; and c) a stiffness of about 10 to about 400 Pascal (Pa).

The invention relates to the technical field of medical instruments for the ophthalmology department, and particularly discloses a preparation method of a biological membrane strip for sclera reinforcement after the ophthalmology department, the preparation method comprises multilayer stacking and edge folding fixing, and specifically comprises the steps that pretreated extracellular matrixes are stacked in multiple layers and placed in a mold, and then four corners are folded inwards to form a fusiform shape; then applying pressure to fix by using upper and lower plane clamping plates, wherein the pressure is 10-200kpa; drying with a mold to obtain a biofilm strip; the middle section of the biological membrane strip is widest and gradually narrows towards two ends in an arc-shaped transition manner; the total length of the biological membrane strip is 20-70mm, and the thickness of the biological membrane strip is 0.1-0.4 mm; the width of the middle section is 10-40 mm, and the length of the middle section is 10-60 mm; and the width of the two ends is 5-20mm. The edge mechanical strength of the prepared biofilm strip is superior to that of the middle section, and the biofilm strip can better meet the requirements of posterior sclera reinforcement surgery for material reliability and long-term stability.

The invention belongs to the technical field of biomedical materials, and discloses an anisotropic nanofiber yarn composite scaffold as well as a preparation method and application thereof. The anisotropic nanofiber yarn composite scaffold is formed by compounding methylacryloyl modified gelatin and a nanofiber yarn scaffold prepared through electrostatic spinning. The anisotropic nanofiber yarn composite scaffold not only has more excellent mechanical properties, but also can provide a more suitable microenvironment for cell adhesion and growth, can significantly improve the remodeling efficiency of an extracellular matrix, and has a wide application prospect in the field of heart valve repair.

The invention belongs to the technical field of tissue engineering, and particularly relates to a bio-ink system for osteochondral repair as well as a preparation method and application of the bio-ink system. The system comprises first bio-ink and second bio-ink which are respectively used for simulating cartilage and bone tissue. The first biological ink is prepared from methacrylic acid gelatin, acellular extracellular matrix powder, aldehyde modified natural polysaccharide, a photoinitiator and a transforming growth factor; the second biological ink is prepared from methacrylic acid gelatin, acellular extracellular matrix powder, nano-hydroxyapatite, aldehyde modified natural polysaccharide, a photoinitiator and human bone morphogenetic protein BMP-2. The bio-ink system can guarantee smooth extrusion and interlayer fusion in the printing process; the constructed bionic microenvironment and the affinity effect of the bionic microenvironment on growth factors are suitable for 3D printing construction of the osteochondral integrated bionic scaffold and have good clinical transformation potential.

The invention discloses a composite multi-oligopeptide composition for skin repair and an application of the composite multi-oligopeptide composition. The multi-oligopeptide composition comprises the following components in parts by weight: 1 to 5 parts of acetyl hexapeptide-8, 0.1 to 1.5 parts of acetyl tetrapeptide-3, 1 to 20 parts of acetyl tetrapeptide-9, 0.1 to 1.5 parts of acetyl tetrapeptide-11, 10 to 190 parts of carnosine, 10 to 100 parts of decarboxylated carnosine hydrochloride, 10 to 120 parts of tripeptide-1, 1 to 50 parts of tetrapeptide-4, 1 to 20 parts of hexapeptide-9, 1 to 10 parts of palmitoyl pentapeptide-4, 1 to 5 parts of palmitoyl tetrapeptide-7, 1 to 10 parts of palmitoyl tripeptide-1, 1 to 35 parts of palmitoyl tripeptide-8, 0.1 to 2.5 parts of oligopeptide-1, 0.1 to 0.5 part of oligopeptide-2 and 0.1 part of carnosine. And 1 part of oligopeptide-5. The multi-oligopeptide composition has excellent anti-wrinkle and anti-aging effects, has the effects of promoting tissue repair and wound healing, strengthening skin barrier and resisting inflammation and relieving, is applied to skin medicine and skin beauty, and has the effects of promoting regeneration and remodeling of an extracellular matrix (ECM), regulating inflammation, blocking inflammatory aging and strengthening a dermis-epidermis joint (DEJ).

The invention provides a small-molecule compound A3 with a good treatment effect on metabolism-related fatty liver diseases, the small-molecule compound A3 is innovatively designed and synthesized based on a pyrene skeleton, and the application of the small-molecule compound A3 in drugs for preventing and / or treating the metabolism-related fatty liver diseases. By establishing a standard in-vivo and in-vitro pharmacodynamic evaluation model, it is proved that the composition has a remarkable intervention effect on the metabolism-related fatty liver diseases. The compound improves the core pathological process of the metabolism-related fatty liver disease through multi-mechanism synergism, can effectively regulate abnormal lipid metabolism, inhibits hepatocyte vacuolation lesion and slows down excessive deposition of extracellular matrix, and shows the advantage of multi-channel treatment.

The application relates to an electrostatic spinning anti-adhesion membrane and a preparation method and application thereof. The electrostatic spinning anti-adhesion membrane comprises an inner layer, an intermediate layer and an outer layer which are sequentially stacked; the inner layer comprises polyglycolide-lactide-polyethylene glycol, the intermediate layer comprises polyglycolide-lactide, and the outer layer comprises polylactic acid-caprolactone. The electrostatic spinning anti-adhesion membrane has a three-dimensional nanofiber network structure of a biomimetic extracellular matrix, excellent mechanical properties, body temperature response softening and gradient degradation characteristics, can balance the degradation speed and the mechanical properties, reduce the mechanical damage of the material to the surrounding tissues, and can effectively prevent postoperative tissue adhesion.

The application provides a red light excitable photoelectric response heterostructure nanotube, a hydrogel and a preparation method and application thereof, and belongs to the technical field of nanomaterials. By integrating a heterostructure, a quantum confinement effect and morphological modification, a one-dimensional TiO2 / Bi2S3 quantum dot nanotube with visible red light excitation and high photoelectric conversion efficiency is prepared, which is combined with a hydrogel precursor to simulate an extracellular matrix, so that a high-performance red light response photoelectric hydrogel dressing is developed. The hydrogel dressing has excellent ability in promoting nerve ending reinnervation, promoting skin substructure repair and regulating immune response in a deep burn model, and has wide application prospects in overall skin repair.

The invention discloses application of water microspheres of modified polysaccharide in promoting wound skin regeneration and inhibiting wound skin fibrosis. In the invention, compared with a Control group (silicone oil) as a control and an unmodified polysaccharide water microsphere group (AVBEC), when the modified polysaccharide water microsphere group (AVBER) is externally applied to the skin of a bama miniature pig in a deep II-level scald model, the wound healing can be effectively promoted, the inflammation degree and duration can be controlled, and the extracellular matrix composition and plasticity can be regulated; expression of fibrosis marker protein is reduced, and the dual effects of promoting skin regeneration and inhibiting fibrosis are achieved. The problems that natural extracts and common polysaccharide microsphere products are low in biological activity and poor in stability are solved, and new functions of modified polysaccharide are found.

The invention relates to the technical field of livestock breeding and animal nutrition, in particular to fattening pig feed and application thereof.The fattening pig feed is prepared from, by weight, 50 parts of corn flour, 19-21 parts of concentrated feed, 9-11 parts of wheat bran and 20 parts of wild buckwheat straw powder. According to the formula, the wild buckwheat straw is used as a functional fiber source, through scientific matching and synergistic effect with conventional energy raw materials, the micro-ecological structure of the intestinal tract of the fattening pig is effectively optimized, enrichment of beneficial microbial communities such as lactobacilli in the phylum firmicalis is promoted, and therefore the steady state of the intestinal tract can be maintained. Meanwhile, in the liver of the fattening pigs fed with the formula, expression of various genes related to extracellular matrix structures and tissue homeostasis is up-regulated, and support is provided for tissue structure maintenance and healthy growth. The method provides a feasible path for resource utilization of wild buckwheat straws, and has double benefits of breeding health promotion and agricultural byproduct value increase.

The invention relates to the field of medical implant materials, and particularly discloses an absorbable patch for uterine muscular layer repair and a preparation method of the absorbable patch. The invention discloses an absorbable patch for repairing a uterine muscular layer. The absorbable patch is prepared by mixing 2-9.5 parts of poly (L-lactide-caprolactone) and 0.5-8 parts of a biomedical material, the molecular weight of the poly (L-lactide-caprolactone) is 5 to 30 thousand; the preparation method comprises the following steps: mixing a biomedical material stock solution with a poly (L-lactide-caprolactone) stock solution, performing electrostatic spinning, freeze drying or tape casting treatment, and drying to remove an organic solvent, thereby obtaining the absorbable patch for uterine muscular layer repair. The absorbable patch disclosed by the invention not only can be completely degraded in vivo, but also has a similar extracellular matrix structure, can provide muscle cell repair for an injured part of a uterine muscular layer, avoids scar tissues formed by fibrosis, and reduces the risk caused by a secondary operation required by a uterine scar diverticulum.

The present disclosure provides polymeric matrices comprising calreticulin (CRbT) and methods of producing and using such matrices. The polymeric matrices are useful in treatment of wounds (e.g., chronic diabetic wound).

The application relates to an antibacterial bionic absorbable wound dressing and a preparation method thereof, and comprises the following steps: (1) preparing a spinning precursor solution: 10-15 wt% of a decellularized extracellular matrix, 3-10 wt% of a natural polymer material, 0.1-3 wt% of a crosslinking agent, and the rest being glacial acetic acid; preparing a spray dispersion liquid: 0.15-2.5 wt% of antibacterial nanoparticles, 0.1-1 wt% of a crosslinking agent, and the rest being an alcohol solvent; the antibacterial nanoparticles are a composite of a natural polymer material embedding antibacterial drugs; (2) placing the spinning precursor solution in an electrostatic spinning injector, placing the spray dispersion liquid in an electrostatic spraying injector, simultaneously carrying out electrostatic spinning and electrostatic spraying after the power is turned on, and obtaining an antibacterial nanofiber membrane on a receiving device; (3) drying and crosslinking the antibacterial nanofiber membrane to obtain the antibacterial bionic absorbable wound dressing, and the microstructure is that the surface of each nanofiber uniformly loads the antibacterial nanoparticles. The dressing is beneficial to cell adhesion and achieves the effect of antibacterial particle slow release.

The present invention relates to a composition and a method for cryogenic grinding of tissue and, more specifically, to a composition and a method for cryogenic grinding of tissue, wherein the composition is applicable to both hard tissue and soft tissue and causes minimal damage to an extracellular matrix by comprising biologically derived tissue and a solid crystalline granular material which is used by being mixed with the biologically derived tissue during cryogenic grinding of the biologically derived tissue and is capable of decellularizing the tissue.

The invention provides an irradiation crosslinking collagen injection filler as well as a preparation method and application thereof. The collagen injection filler comprises collagen gel particles, and the collagen gel particles are of a porous structure. The collagen gel particles have connected fiber structures, the fiber structures are formed by collagen self-assembly, and the fiber structures comprise fiber bundles and / or fiber mesh sheet structures; the collagen gel particles in the collagen injection filler have fiber structures formed after collagen self-assembly, that is, collagen is spontaneously assembled from collagen molecules to fiber bundles and fiber mesh lamellar structures, the mechanical property of a fiber network is improved, and the degradation rate is delayed. The fiber network structure provided by the invention has good mechanical support performance and slow degradation rate, simulates a three-dimensional microenvironment of a natural extracellular matrix (ECM), and promotes cell attachment proliferation and tissue regeneration. The fiber structure is beneficial to creeping proliferation of cells and promotes regeneration and repair of skin cells.

The application belongs to the technical field of new materials, and discloses a transdermal drug delivery device and a preparation method and use method thereof. The transdermal drug delivery device comprises a flexible patch and a power supply. The flexible patch comprises a flexible substrate and a liquid metal electrode. The liquid metal electrode is embedded in the flexible substrate, and the flexible patch is modified by extracellular matrix proteins. The power supply is connected with the flexible patch. The use method is as follows: a drug is coated on the surface of skin, the transdermal drug delivery device is covered on the skin surface coated with the drug, the power supply is turned on, and the transdermal drug delivery device realizes the penetration of the drug into the skin through iontophoresis. The device simply connects the flexible patch with the power supply, realizes the miniaturization and portability of iontophoresis therapy, does not harm the skin, is efficient and safe, and has high patient compliance.

This invention relates to a biomimetic extracellular matrix hydrogel, its preparation method, and its application. The preparation method of this invention includes the following steps: (1) dissolving a natural high-molecular-weight polysaccharide in solvent A, then adding a halogenated oxyacid salt, and reacting in the dark; after the reaction is completed, adding ethylene glycol to terminate the reaction, dialyzing, and then freeze-drying to obtain GM-CHO powder; (2) dissolving the GM-CHO powder in solvent B, adding an amino acid polymer, and stirring at a constant temperature; then dialyzing and freeze-drying sequentially to obtain GMI glycopeptide; (3) dissolving the GMI glycopeptide and aminated glucomannan in solvent C, vortexing, and finally allowing it to stand at room temperature to form a biomimetic extracellular matrix hydrogel. The biomimetic extracellular matrix hydrogel of this invention can simultaneously achieve "correcting metabolic disorders, clearing pathogens, and simulating extracellular matrix", improving the retention time at the wound site and facilitating long-term therapeutic effects.

To provide a gel composition that is transparent and has a high modulus of elasticity, containing extracellular matrix components and / or fragmented extracellular matrix components. [Solution] A gel composition containing at least one selected from the group consisting of extracellular matrix components and fragmented extracellular matrix components, and ions of a metal element.

The present disclosure relates to a method for producing an extracellular matrix hydrogel, the method comprising (a) decellularizing a tissue sample to form a decellularized tissue mesh; (b) depolymerizing the decellularized tissue mesh to form a depolymerized protein dispersion, the depolymerizing comprising mixing the decellularized tissue mesh with a depolymerizing solution comprising a chaotropic agent to form a depolymerizing mixture; mechanically homogenizing the depolymerizing mixture at a chilled temperature to form a homogenized mixture; separating the homogenized mixture into (a) a waste precipitate and (b) a supernatant comprising the depolymerized protein dispersion; (c) filtering the depolymerized protein dispersion to form a sol; and (d) gelling the sol to form the extracellular matrix hydrogel, the gelling comprising combining the sol with a gelling solution comprising a kosmotropic agent; and separating a fluid phase away from the extracellular matrix hydrogel as it forms.

The invention discloses a pharmaceutical composition containing a tumor-derived acellular extracellular matrix and an application of the pharmaceutical composition. The invention provides a strategy for regulating and controlling tissue regeneration based on a tumor-derived acellular extracellular matrix microenvironment, a novel angiogenesis promoting biological material is constructed by combining the high similarity of a tumor microenvironment and a regeneration microenvironment such as angiogenesis, and high-efficiency regeneration is realized in various tissue defects. In addition, due to the characteristic of rapid proliferation of the tumor tissue, the productivity bottleneck of a traditional acellular extracellular matrix raw material source is broken through, and sustainable biological raw materials are provided for large-scale production. The method successfully solves the problem that a specific tissue-derived acellular extracellular matrix is difficult to regenerate in a complex damaged microenvironment.

This invention discloses a biomimetic extracellular matrix composition, its preparation method, application, and cosmetics, belonging to the field of cosmetic technology. The composition comprises, by weight percentage, the following components: Type I collagen: 0.01%-0.5%, Type III collagen: 0.01%-0.3%, Type VII collagen: 0.005%-0.2%, Type XVII collagen: 0.005%-0.2%, elastin: 0.01%-0.3%, fibronectin: 0.005%-0.2%, moisturizing ingredients: 0.2%-2.0%, repair factors: 0.061%-0.8%, polypeptide ingredients: 0.02%-0.8%, β-Xylane: 0.5%-3.0%, excipients: 0%-5%, and the balance being water. This invention uses four types of collagen, elastin, and fibronectin as the core structure, and combines them with moisturizing ingredients, repair factors, and peptides to form a complete synergistic system. This system forms a multi-pathway, multi-level synergistic enhancement system that can biomimetically reconstruct the extracellular matrix structure of the entire skin layer, achieving six effects in one: anti-aging, firming, wrinkle reduction, repair, moisturizing, and brightening.

This invention provides a camellia seed diglyceride microcapsule, its preparation method, and its application, belonging to the field of cosmetic raw material preparation technology. The invention uses a three-dimensional network cross-linked polymer of sea fennel extract, BOXER, and recombinant collagen as the wall material. Recombinant collagen provides the macromolecular framework, and BOXER can cross-link with the recombinant collagen, optimizing the three-dimensional network structure of the wall material and filling the cross-linking sites. The polyphenols contained in the sea fennel extract participate in hydrogen bonding / covalent cross-linking, forming a dense three-dimensional network structure, increasing the mechanical strength of the wall material and encapsulating the camellia seed diglyceride core material, thus improving the stability and antioxidant properties of the camellia seed diglyceride. Sea fennel extract has excellent repair and anti-aging effects, promoting skin cell regeneration; BOXER can promote the synthesis of extracellular matrix glycosaminoglycans, strengthening skin firmness; and recombinant collagen can replenish lost collagen in the skin, improve skin elasticity, and enhance the anti-aging performance of the camellia seed diglyceride microcapsules.