182 results about "Extracellular ground substance" patented technology

The invention relates to a tissue engineering material and a preparation method thereof, in particular to a nano fiber tissue engineering blood vessel and a preparation method thereof. The invention consists of a three-dimensional reticular non-woven film formed by an inner layer of nano fiber and an outer layer of nano fiber; the inner layer of the blood vessel is natural polymer, wherein, calculated by weight, 40 percent to 80 percent is fibroin, 20 percent to 50 percent is gelatine, 0 percent to 20 percent is extracellular matrix protein; while the outer layer of the blood vessel is synthetic polymer. The preparation method is that the natural polymer is dissolved in trifluroroethyl and other solution, while the synthetic polymer is dissolved in hexafluoroisopropanol and other solution, which are respectively prepared into spinning solution; the static electricity spinning technique is adopted to subsequently form the inner and the outer layers on a gather roller; cross-linked treatment is conducted after the inner and the outer layers are taken down, to prepare the nano fiber tissue engineering vessel. The inner layer can simulate the structure of the extracellular matrix, provide good environment for endothelial cells to grow, support adhesion, proliferation and differentiation of the cells, and is good for endothelization of the blood vessel; and the outer layer has good mechanical performance.

The invention discloses a humanization active forging bone and a preparation method thereof. The humanization active forging bone has main components of hydroxyapatite crystal, natural animal cancellous bones, particles of which the particle size is between 0.1 and 5 millimeters and having three-dimensional netlike porous structures, and a coating of which the pore-size distribution is between 50 and 600 microns and the surface is compounded with extracellular matrix synthesized and excreted by human cells. The forging bone not only has a similar structure with a human bone and is advantageous for cell ingrowth, formation of new bones, and bone structure reconstruction at a transplantation position, but also can be firmly combined with the coating of which the surface is coated with the extracellular matrix synthesized and excreted by the human cells and cell growth factors, promotes the attached growth of in vivo osteoblasts, and accelerates the formation of the new bones; and simultaneously, the forging bone has the advantages of molding randomly and not causing immunological rejection reactions, and can satisfy treatment needs of clinical bone defect and nonunion.

The invention relates to a preparation method of a porous scaffold material and discloses a preparation method of a nano-fibrous silk fibroin-based porous scaffold. The preparation method comprises the following steps: blending a silk fibroin solution and a collagen solution under the temperature of 0-20 DEG C, standing to ensure the complete reaction of collagen and silk fibroin and generate nano-fiber; freezing the mixed solution to obtain a freezing body; performing freeze drying treatment on the freezing body to obtain a nano-fibrous porous scaffold material; and placing the nano-fibrous porous scaffold material in a vacuum drier with water and methanol, or an ethanol aqueous solution at the bottom, vacuumizing, and then standing to obtain the water-insoluble nano-fibrous porous scaffold. The obtained porous scaffold has 10-1000 microns of communicated pores. The pore walls consist of 10-100nm of nano-fibers; and the porous scaffold has similar nanoscale structures and micronscalestructures to extracellular matrix, is suitable for the adhesion and growth of cells and has good biocompatibility.

The invention discloses oxidized graphene doped nano-fibers, as well as a preparation method and application thereof. The nano-fibers are continuous nano-fibers with smooth surfaces and mainly formed by oxidized graphene and poly(lactic-co-glycolic acid) using an electrostatic spinning method. The nano-fibers provided by the invention can be used to form a porous three-dimensional structured nano-fiber scaffold material for tissue engineering. The scaffold material for tissue engineering simulates cell natural extracellular matrix, wherein the diameter of the nano-fiber ranges from a dozen nanometers to several micrometers, and the nano-fiber is similar with the collagen fiber in the extracellular matrix; the scaffold material provided by the invention has a porous topology structure with a high specific surface area and simulating the extracellular matrix, can provide better growth environment for the cells, can remarkably promote the adhesion, proliferation and differentiation of the stem cells; at the same time, the preparation technology of the nano-fibers is simple without using complicated equipment; mass production can be achieved, and the manufacturing cost is low.

An articular cartilage graft and a preparation method thereof are provided. The prepared articular cartilage graft is composed of a superficial layer, a middle layer and a deep layer from outside to inside; the thickness, shape and size of each layer are all matched with a cartilage injury part, and thus preoperative shaping is not required; after grafting, the articular cartilage graft has layer distribution corresponding to distribution of each layer of surrounding normal cartilages, is conducive to intercellular signal transmission, transduction and regulation, and can be better integrated with surrounding normal cartilage tissues; the articular cartilage graft has structure characteristics consistent with those of the natural cartilages, the collagen type II content is gradually decreased from the superficial layer to the deep layer, the GAG content is increased gradually from the superficial layer to the deep layer, and compression resistance and wear resistance are good; and chondrocytes in the cartilage graft are wrapped with an extracellular matrix, have low immunogenicity, allow generation of immunologic rejection to be avoided after grafting, can survive for a long term and exert functions, and improve cartilage repair long-term curative effects.

The invention provides a preparation method for a novel biomimetic matrix type biological wound healing material. According to the method, small intestinal submucosa with a three-dimensional scaffold structure is compounded with platelet-rich blood plasma containing a specific proportion of bioactive factors, and a specific process is adopted to carry out modification to form the novel material containing biological induction factors, wherein the novel material has a three-dimensional biomimetic structure. With the biomimetic matrix type biological wound healing material prepared by the method of the present invention, the healing can be promoted, the physiological repair can be promoted, the pathological repair can be inhibited, and the regeneration repair efficiency and the repair quality of the damaged tissue can be improved. The novel biological wound healing material of the present invention can be used for repairs of various human soft tissues, membranous tissues or cavity walls, and for cavity filling, and can be used as the ideal extracellular matrix biomimetic material and the cell scaffold material for wound repairs of the tissues and the organs, promotion of wound healing, prevention of adhesion and the like.

The invention discloses a nerve repairing film and a preparation method thereof. The method mainly comprises the steps of treating raw materials, decellularizing, dissolving matrix, drying to form a film, neutralizing and washing and the like. According to the nerve repairing film prepared by the method, active ingredients of the extracellular matrix are maintained, the peripheral nerve suture is directly coated, so that an excellent microenvironment is provided to nerve regeneration, nerves are prevented from adhering to peripheral tissues, and nerve regeneration is promoted.

The invention discloses a method for preparing a nanofiber by electrostatic spinning. The method comprises the following steps: (1) preparing a pullulan-gelatin mixed solution, namely dissolving a pullulan and gelatin mixture in de-ionized water and magnetically stirring until fully dissolving the pullulan and gelatin mixture; (2) electrostatically spinning, namely performing electrostatic spinning on the pullulan-gelatin mixed solution obtained in the step (1), adjusting process parameters, and receiving by adopting a plane receiving device, wherein the process parameters are as follows: voltage in electrostatic field is 15-25 kv, the spinning speed is 0.3-0.7 ml/h, the receiving distance is 8-20 cm and the relative humidity is 20-50%. The method adopts water as a unique solvent, is free of organic solvent residues, prepares the nanofiber through the electrostatic spinning process, and can carry out biomimetic treatment on a natural extracellular matrix in composition and structure.

Provided is an organic/inorganic hybrid-porous biodegradable polymer scaffold, which includes basic ceramic nanoparticles, an animal tissue or cell-derived bioactive first extracellular matrix material, and a biodegradable polymer and in which inner walls of pores are coated with a second extracellular matrix material.

The invention provides a preparation method of an animal bladder decellularizing matrix. The preparation method comprises the steps of extracting a strata submucosum of an animal bladder, and carrying out virus inactivation, degreasing and decellularization, wherein the decellularization step sequentially comprises a repeated freeze thawing step and a decellularization solution decellularization step, the freezing temperature in the freeze thawing step is (-70)-(-90) DEG C, and the freezing time is 5-15 hours. The invention further provides the animal bladder decellularizing matrix prepared by virtue of the preparation method and the application of the animal bladder decellularizing matrix. According to the preparation method, the bladder decellularizing matrix is prepared by combining the repeated freeze thawing with the decellularization solution decellularization, elastic fibers and a collagen net structure of an extracellular matrix are furthest preserved, and meanwhile, antigens are thoroughly removed. The animal bladder decellularizing matrix can be applied to the repairing fields of bladders, urethrae and ureters.

The invention discloses a short peptide. The sequence of the short peptide is (arginine-alanine-aspartic acid-alanine)4-arginine-isoleucine-lysine-valine-alanine-valine, (arginine-alanine-aspartic acid-alanine)4-lysine-isoleucine-lysine-valine-alanine-valine or (arginine-alanine-aspartic acid-alanine)4-histidine-isoleucine-lysine-valine-alanine-valine. The invention simultaneously discloses a functional self-assembling nano-polypeptide hydrogel material prepared from the short peptide. The hydrogel material has a shear thinning characteristic, can immediately form hydrogel after being injected to a bleeding point through an injector, can be applied to rapid hemostasis and is particularly applied to the situation that the bleeding point environment is complicated; and the short peptide can form nano-fiber hydrogel at the room temperature when the pH value is neutral and can simulate a natural extracellular matrix structure and has good physical and chemical properties and biocompatibility.

A hydrogel-forming composition is provided that comprises an extracellular matrix protein, hyaluronic acid, and a thermosensitive biocompatible polymer such as methylcellulose. The hydrogels can provide a therapeutic effect; further, the hydrogels may comprise an optional therapeutic agent such as cells or a pharmaceutical composition. The composition may be injected to an area in need of treatment by the therapeutic agent. The composition may form a gel at about 37° C., such that the gel maintains the therapeutic agent in the area of the body in need of such treatment.

The invention relates to a fibroin three-dimensional bracket for nervus centralis remediation, and belongs to the field of biological medicinal materials. The fibroin three-dimensional bracket comprises fibroin protein fiber with grooves, fibroin protein/hyaluronic acid plural gel and fibroin membrane conduits. By adopting the fibroin three-dimensional bracket, defects that a conventional bracket is low in mechanical property, low in biodegradability and the like are overcome. By adopting the fibroin three-dimensional bracket, physiological states of an extracellular matrix can be simulated, adhesion, migration, amplification and differentiation of cells can be facilitated, and furthermore orientated growth of axon to fracture parts can be guided; due to connection of the bracket, glial scars can be inhibited, and thus remediation of a nervus centralis system can be facilitated; and the bracket can be applied to basic research on neural injury remediation, can be also applied to bridging remediation of human body spinal cord injury or deficiency, and has good biomedical prospects.

The invention discloses a hydroxypropyl tetrahydropyranitol compound and a preparation method and application thereof. The hydroxypropyl tetrahydropyranitol compound comprises hydroxypropyl tetrahydropyranitol, hyaluronic acid and/or salts thereof, hydrolyzed hyaluronic acid and/or salts thereof, acetyl chitin (hyaluronic acid precursor), deionized water and a component with a preservative effector a preservative. The compound is capable of strengthening the connection between epidermis and dermis of skin, thereby improving the expression and content of fibroblast in dermis to extracellular matrix, such as collagen, proteoglycan and glycosaminoglycan and improving the skin elasticity and the skin firmness to achieve the anti-aging function of the skin. Through the addition of a certain proportion of low-content hyaluronic acid and/or salts thereof, hydrolyzed hyaluronic acid and/or salts thereof and acetyl chitin (hyaluronic acid precursor) into the compound of employing the hydroxypropyl tetrahydropyranitol as a main component, and through an appropriate proportion, the cooperativity of various components in efficacy can be put into a full play, and a moisturizing function, an anti-aging function and other functions of the components for skin care are developed to a greater extent.

The invention discloses a preparation method of a silk fibroin tissue engineering scaffold based on a near-field electrospinning technology. The preparation method comprises the steps that a regenerated silk fibroin aqueous solution is mixed with a polyoxyethylene aqueous solution, the ratio of the mixed materials is adjusted, the viscosity characteristic of the whole mixture is changed, and a silk fibroin/PEO solution system which can be used for near-field electrospinning is prepared; then the near-field electrospinning printing technology is adopted, printing is carried out according to a scaffold model of a cross-shaped net structure circulating in a periodic cycle, and finally, the preparation of the silk fibroin tissue engineering scaffold is realized through a post-treatment processsuch as chemical modifier soaking. The preparation method is simple, the production efficiency is high, the inherent characteristics of the raw materials are not affected, the porosity of the prepared tissue engineering scaffold is high and adjustable, the pore size is proper, the mechanical property is good, and the specific surface area is large; and the biocompatibility and the biological activity of the scaffold material are good, the extracellular matrix can be simulated well, and the scaffold material is suitable for cell adhesion, proliferation and differentiation.

The invention belongs to the field of biological materials for tissue engineering repair and tissue regeneration promotion, and particularly relates to a preparation method and application of a blended hydrogel biological scaffold material with biological activity. The blended hydrogel biological scaffold material is prepared by sequentially mixing an acellular bone meal matrix and methacrylate-modified gelatin according to a certain proportion. The preparation method of the scaffold material provided by the invention is safe, non-toxic and easy to operate, the acellular bone meal with low immunogenicity is obtained by performing acellular treatment on bones of alpha-1,3-galactoside gene knockout pigs, and the acellular bone meal has the characteristic of low immunogenicity after being implanted into a living body. The tissue engineering scaffold material is obtained by blending the acellular bone meal with biological activity and low immunogenicity with the GelMA hydrogel, the hydrogel material mixed with the acellular bone meal reserves the extracellular matrix of a natural material, the hydrogel can also provide a good supporting effect for the scaffold material, the biocompatibility is good, and thereby the scaffold material can be used as a bone tissue engineering material to be applied to hard bone defects in vivo, skull repair and the like. Compared with a traditional treatment method, the scaffold material has wide application prospects.

The invention discloses an antibacterial wound-protecting hydrogel dressing and a preparation method thereof. The antibacterial wound-protecting hydrogel dressing comprises the following main components: a water-soluble chitosan derivative, matrix glue and a water-soluble high-molecular polymer. The preparation method comprises the following steps: preparing a chitosan derivative from purified water, fully and uniformly mixing the components, adding the chitosan derivative into a mold, adding a liquid matrix gel and an acrylamide solution, sequentially adding a crosslinking agent, an initiatorand an accelerator to form gel, and rinsing the gel with deionized water to remove the redundant crosslinking agent, thereby obtaining the hydrogel dressing. The dressing can absorb wound exudate andsecreta. The chitosan derivative has polycation, so that when being combined with negatively charged protein, teichoic acid and the like on the surface of thallus, the chitosan has strong antibacterial activity, and has unique advantages in prevention and treatment of wound infection. Matrix glue is one of extracellular matrixes, contains various bioactive components and plays an important role in the wound healing process. The hydrogel dressing can be applied to protection and repair of various types of wounds such as burns, wounds, pressure sores and the like.

The invention relates to an intelligent anti-aging composition, a preparation process thereof, and application thereof in cosmetics. According to the composition, camellia oil, components such as a radix angelicae sinensis extract and a citrus extract are inserted into a lipidosome bilayer, an intelligent anti-aging component is wrapped, and the intelligent slow release of anti-aging function components is realized through the change of illumination intensity; the anti-aging components wrapped by lipidosome is innovatively compounded aiming at multiple mechanisms produced by skin aging, has the efficacies of moisturizing and protecting skin, promoting cell proliferation, resisting ultraviolet light, scavenging free radical, inhibiting MMPs expression, moisturizing, complementing extracellular matrix and the like, and produces a synergistic interaction effect. The intelligent anti-aging composition provided by the invention realizes the aims of intelligently slow releasing the anti-aging function components, improving the product efficacy and the safety, prolonging the action time, reducing the skin load, and the like; the composition can be widely applied in cream milk agents, emulsion and solution cosmetics.

The invention discloses a preparation method of medical PLLA (poly-l-lactic acid) melt-spun fibers. The preparation method comprises steps as follows: medical PLLA with good biodegradability and biocompatibility is sliced, dried, extruded by a screw extruder with a single-orifice plate, a 36-orifice plate or a 24-orifice plate for melt spinning and drafting winding, and the medical PLLA melt-spun fibers are prepared. The medical PLLA melt-spun fibers prepared with the method has smaller fiber diameter, smooth surface, circular cross section and higher melting point. The medical PLLA melt-spun fibers have better application prospect, can be used for simulating the fiber network structure in an ECM (extracellular matrix), and can be used for preparing fiber scaffolds applied to tissue engineering as well as woven fabric and knitted fabric scaffolds applied to blood vessels, nerves, ligaments, muscle, knees, tendons, bone tissue engineering, drug release and loading and the like.

The invention discloses a preparation method of antagonist functionalized poly-L-lactic acid porous microspheres. The preparation method comprises the following steps: (1) preparing antagonist-loadedBSA nanoparticles; (2) preparing poly-L-lactic acid porous microspheres: taking L-polylactic acid, gelatin and polyvinyl alcohol as raw materials, preparing the microspheres by using a microfluidic device, treating the microspheres in alkaline water, and cleaning the microspheres with deionized water to obtain the poly-L-lactic acid porous microspheres; and (3) preparing the antagonist functionalized poly-L-lactic acid porous microspheres: coupling BSA nanoparticles loaded with an antagonist with the poly-L-lactic acid porous microspheres by adopting an EDC/NHS method to prepare the antagonistfunctionalized L-polylactic acid porous microspheres. A series of material science tests, cell experiments and in-vivo experiments prove that the porous microspheres have good biocompatibility and anti-inflammatory performance, extracellular matrix metabolic imbalance caused by inflammation is corrected, and tissue function recovery is promoted.