214 results about "Extracellular ground substance" patented technology

The invention discloses a preparation method of a controllable silk fibroin extracellular skeleton gel material for injection. The composite filler is prepared from the following raw materials in parts by mass: 5-50 parts of silk fibroin, 0.01-5 parts of cellulose and 0.7-10 parts of hyaluronate. The preparation method comprises the following steps: (1) preparation of silk fibroin microfiber gel: adding cellulose into a silk fibroin aqueous solution to prepare gel, crushing and sieving to obtain the silk fibroin microfiber gel; and (2) preparation of an extracellular skeleton gel material: mixing hyaluronate with the silk fibroin microfiber gel, and carrying out physical crosslinking to form the silk fibroin extracellular skeleton gel material. After the modified silk fibroin hydrogel prepared by the method is injected, in-situ tissue regeneration can be promoted, the molecular weight is controllable, the crosslinking degree is controllable, the structure of the modified silk fibroin hydrogel is similar to that of an extracellular matrix (ECM), the degradation process is matched with the in-situ tissue regeneration process, and the modified silk fibroin hydrogel is well improved in the aspects of mechanical property, degradation performance, biocompatibility and the like and has good application prospects. No chemical cross-linking agent is used, and the injection has injectability, and has a very good application prospect in the field of medical beauty injection.

The invention relates to a double-layer dressing for promoting wound healing and inhibiting scars and a preparation method and application thereof.The double-layer dressing comprises a hydrogel layer and a piezoelectric film layer compounded on the hydrogel layer, and the piezoelectric film layer is prepared by mixing an organic piezoelectric material, a ferroelectric material and an organic solvent and then conducting an electrostatic spinning technology; the hydrogel layer is formed by curing a methacrylic anhydride acellular extracellular matrix, methacrylic anhydride catechol chitosan and conductive nanoparticles under the action of a curing agent. The lower skin contact layer of the double-layer dressing is the hydrogel layer, so that the directional growth of fibroblasts is favorably induced; the upper dressing is a piezoelectric material composite ferroelectric material, is beneficial to piezoelectric stimulation re-induction function recovery after final skin repair and guidance of directional growth of wound cells, and has good skin wound induction repair and scar inhibition effects.

OF THE INVENTION Provided herein is a method of preparing an extracellular matrix (ECM) material, including incubating vaginal tissue in a protease, incubating the vaginal tissue in a solution including at least one detergent, incubating the vaginal tissue in a composition including at least one disinfecting agent, thereby producing a decellularized ECM material, lyophilizing the decellularized ECM material, thereby producing a lyophilized ECM material, comminuting the lyophilized ECM material, partially or completely solubilizing the comminuted, lyophilized ECM material with an acid protease to produce solubilized ECM, lyophilizing the solubilized ECM to produce lyophilized, solubilized ECM.

The invention discloses an ultrasonic molecular probe. The ultrasonic molecular probe comprises a targeted contrast agent and siRNA of fibronectin, wherein the targeted contrast agent and the siRNA are connected through G0-C14; the targeted contrast agent comprises gas vesicles covalently linked with annular polypeptide, and the annular polypeptide targets an atherosclerotic plaque region. The cyclic polypeptide can be combined with the outer structural domain B of fibronectin with high specificity, and the atherosclerotic plaque extracellular matrix is rich in the outer structural domain B of the fibronectin, so that enough targeted ultrasonic contrast agent can be quickly combined in the plaque region to generate strong signal enhancement in ultrasonic imaging, and on the basis, the targeted ultrasonic contrast agent can be quickly combined with the plaque region to generate strong signal enhancement in ultrasonic imaging. The ultrasonic molecular probe releases siRNA for atherosclerotic plaque treatment, and atherosclerotic plaque diagnosis and treatment integration is achieved. The invention further discloses a preparation method of the ultrasonic molecular probe and application of the ultrasonic molecular probe in preparation of atherosclerotic plaque diagnostic agents, drugs and drug delivery agents.

The present invention relates to a preparation method of a collagen filler for injection. Non-crosslinked collagen, crosslinked collagen, and biodegradable polymer microspheres are compounded and mixed in a certain proportion to achieve an immediate filling effect. The biodegradable polymer microspheres play a long-term role, and the amino acid components degraded from collagen in the early and middle stages can become nutrients that stimulate the production of collagen regeneration by polyester microspheres. In the present invention, the crosslinked collagen has a high yield and less water loss through a gradient irradiation crosslinking technique. The non-crosslinked collagen gel can provide a microenvironment for nutritional repair and a filling effect in the early stage, the crosslinked extracellular matrix can provide a repair microenvironment in the middle stage of filling, and the degradation of polyester microspheres in the later stage promotes collagen regeneration, enabling the skin to have a good microenvironment throughout the repair cycle and increasing skin elasticity.

The invention discloses an extracellular matrix-imitated anti-inflammatory nerve repair-promoting conductive hydrogel as well as a preparation method and application thereof, and belongs to the technical field of high polymer materials. The preparation method of the conductive hydrogel comprises the following steps: preparing an MXene nanosheet dispersion liquid, and sequentially adding polyphenol and magnesium ions into the MXene nanosheet dispersion liquid to obtain a modified nanosheet solution; mixing the modified chitosan with polyvinyl alcohol to obtain a solution A; mixing and reacting the modified nanosheet solution with phenylboronic acid modified gelatin to obtain a solution B; mixing the solution A and the solution B for reaction, refrigerating at low temperature, and unfreezing to obtain the conductive hydrogel. The hydrogel prepared by the invention has excellent conductivity, and the obtained conductive hydrogel has the functions of resisting inflammation and promoting nerve repair. The bionics method not only improves the diversity and functionality of biological materials, but also provides new possibility for future tissue engineering and regenerative medicine.

An inflated and suspension dried decellularized extracellular matrix of a mammalian organ or tissue, or a vascularized portion thereof, and methods of making and using the inflated and suspension dried material, are provided.

The invention relates to the field of biomedical application of inorganic materials, in particular to application of lanthanum carbonate in preparation of drugs for resisting liver cirrhosis and inhibiting liver inflammations, the drugs comprise lanthanum carbonate and pharmaceutically acceptable auxiliary materials, the drugs are ground and then mixed with food to prepare a drug mixture, and the drug mixture is prepared into the drug for resisting liver cirrhosis and inhibiting liver inflammations. The mass ratio of the lanthanum carbonate in the medicine mixture is 1-10%, and the mechanism of the lanthanum carbonate for treating the liver cirrhosis is as follows: interference or blocking of activation and proliferation of hepatic stellate cells, inhibition of extracellular matrix generation and promotion of extracellular matrix degradation. According to the invention, a rat liver cirrhosis model is constructed through chemical induction, and the effects of reversing liver cirrhosis and inhibiting liver inflammation are found when a rat is treated by taking lanthanum carbonate orally, so that a new candidate drug is provided for clinical treatment of liver cirrhosis.

The invention relates to the technical field of bone tissue materials, and discloses a metal-doped biomimetic mineralized dECM cryogel and a preparation method and application thereof.The preparation method of the cryogel comprises the steps that firstly, hyaluronic acid and methacrylic anhydride are mixed in a solvent, the pH value is adjusted, a reaction is conducted, and methacrylic acid hyaluronic acid is obtained; then under the condition of a photoinitiator, mixing the methacrylic acid hyaluronic acid with an acellular extracellular matrix, and performing freezing and crosslinking treatment to obtain the cryogel, and finally, soaking the cryogel in a mineralization solution containing Ca < 2 + > and / or Mg < 2 + >, adding a buffer solution containing phosphite ions, incubating, and washing to obtain the crystal gel. The biomimetic mineralized dECM cryogel prepared by the method disclosed by the invention is good in ion release stability, can significantly improve the migration ability of HUVECs, contributes to promoting proliferation of rMSCs and improving the osteogenic differentiation potential of the rMSCs, and can be used for regeneration and repair of bone defects.

The invention discloses a high-adhesion enzymatic hydrogel for promoting vascular intima repair and a preparation method of the high-adhesion enzymatic hydrogel, and belongs to the technical field of hydrogel preparation. The preparation method comprises the following steps: S1, carrying out double-bond modification on hyaluronic acid by utilizing methacrylic anhydride, and then cross-linking with dopamine to prepare dopamine modified double-bond hyaluronic acid; s2, deoxidizing the hyaluronic acid solution, adding a cross-linking agent and tyramine hydrochloride, and reacting in an acidic environment to prepare tyramine modified hyaluronic acid; s3, mixing the dopamine modified double-bond hyaluronic acid, the tyramine modified hyaluronic acid and deionized water to prepare a hydrogel precursor liquid; and S4, carrying out enzymatic reaction to obtain the hydrogel. The method designed by the invention is simple in process, no extra photocuring equipment needs to be added, and the constructed hydrogel is similar to an extracellular matrix. And the coating can be coated into different shapes to adapt to different environments. The gel is formed through enzymatic reaction, and the gel is rapid in gel forming, good in tissue adhesion, good in biocompatibility and suitable for being applied to vascular repair.

The invention relates to the technical field of medicines, in particular to application of alpha-ketoglutaric acid in preparation of a medicine for preventing and / or treating myopia. The invention provides application of alpha-ketoglutaric acid or pharmaceutically acceptable salt thereof in preparation of products for preventing and / or treating myopia, and the alpha-ketoglutaric acid is taken as an important intermediate metabolite of tricarboxylic acid cycle in mitochondrial aerobic respiration and has oral safety; researches show that alpha-ketoglutaric acid can inhibit myopia progress and ocular axis elongation by improving sclera fibroblast phenotype transformation and extracellular matrix remodeling, and a new idea is provided for preparation of myopia intervention drugs.

An anti-aging composition for remodeling a dermal structure, a preparation method therefor and a use thereof, relating to the technical field of cosmetics. The anti-aging composition provided comprises the following components in parts by mass: 0.1-5 parts of an alpinia zerumbet leaf extract, 0.01-2 parts of a mixed solution of sodium mannose phosphate and mannose, and 0.01-0.2 parts of retinol substances. The composition provided can promote the generation of collagen and elastin in a reticular layer, enhance the cohesion, enable the reticular layer to have a more compact structure and fiber bundles to be more tightly woven, and promote fibroblast proliferation and metabolism to prevent the extracellular matrix from disintegration, thereby completing remodeling of the dermal structure, toughening the dermal-epidermal junction, and achieving the effect of lifting and firming the skin. The composition can also be prepared into an essence to achieve the effects of ameliorating skin dullness and repairing the skin barrier. The preparation methods for the anti-aging composition and the essence are simple, the raw materials are easily obtained, and the actual production is easy.

The method of treating obesity and targeting adipose tissue in a patient may use injection of a polycationic polymer, such as polyamidoamine (PAMAM) generation 3 (P-G3) or a PCL-g-PAMAM Denpol, that when delivered intraperitoneally, selectively targets visceral adiposity due to its high charge density. P-G3 treatment of obese mice inhibits visceral adiposity, increases energy expenditure, prevents obesity, and alleviates associated metabolic dysfunctions. The extracellular matrix of adipose tissue is enriched with glycosaminoglycans, the known biomacromolecules with the strongest negative charge. P-G3 uncouples adipocyte lipid synthesis and storage from adipocyte development, creating adipocytes with normal functions but that are deficient in hypertrophic growth. The visceral fat distribution of P-G3 is further enhanced by modifying P-G3 with cholesterol to form lipophilic nanoparticles, effective in treating obesity. This strategy provides a method to target visceral adiposity, and cationic nanomaterials useful for treating metabolic diseases or for delivery of additional treating agents.

The invention relates to an anti-aging composition and application thereof. The anti-aging composition is prepared from the following raw materials: dendrobium officinale, camellia japonica and polygonatum kingianum. Dendrobium officinale, camellia japonica and polygonatum kingianum cooperate with one another to achieve a synergistic effect, secretion of type I collagen (COL-1) can be promoted, secretion of matrix metalloproteinase-1 (MMP-1) can be inhibited, and decomposition of collagen in extracellular matrixes can be slowed down. The anti-aging composition can increase skin elasticity, improve the skin barrier function, reduce the percutaneous water loss rate and delay skin aging.

The invention discloses hydrogel bio-ink, a preparation method of the hydrogel bio-ink, a 3D printing biodegradable airway stent and application of the 3D printing biodegradable airway stent. The preparation method comprises the following steps that S1, lung tissue is subjected to freeze thawing, slicing, sequential decellularization of SDS and TritonX-100, air drying, smashing and cryopreservation, and a lung extracellular matrix is obtained; s2, performing sterilization and pepsin treatment on the lung extracellular matrix prepared in S1 to obtain a pretreated lung extracellular matrix; and S3, mixing methyl propionylated gelatin and an alginate solution, pouring the mixture into solid gel, gelatinizing the solid gel, and adding the pretreated lung extracellular matrix to prepare the hydrogel bio-ink. The prepared pig lung acellular extracellular matrix does not have an obvious nuclear structure, and DNA (Deoxyribonucleic Acid) determination shows that the concentration of an acellular group is obviously reduced (P is less than 0.01); along with the increase of the dECM concentration, the porosity is reduced, and the degradation time is prolonged; the airway stent is printed in a 3D biological printing mode, no obvious cytotoxicity (P is larger than 0.05) exists in a cytotoxicity experiment, and the mechanical property part of the airway stent is similar to that of a silicone stent.

The invention belongs to the technical field of medical biological material preparation, and particularly relates to a sterile medical wound repair hydrogel dressing which takes dual-modified hyaluronic acid, polyvinyl alcohol, nano zinc, a humectant and a lubricant as main raw materials. The preparation method comprises the following steps: firstly, utilizing dual-modified hyaluronic acid as a core skeleton of a hydrogel system, and jointly constructing a double-layer network structure through free radical polymerization and crosslinking of boric acid ester bonds and carbon-carbon double bonds formed between phenylboronic acid groups and polyvinyl alcohol; meanwhile, polyvinyl alcohol and zinc ions form coordinate bonds which are interpenetrated in a network, so that the hydrogel dressing with a triple network structure is formed. The unique network structure can simulate an extracellular matrix microenvironment and is beneficial to promoting cell proliferation. In the using process, the hydrogel dressing can play a role in integrating multiple functions. Firstly, the hydrogel dressing can be attached to the surface of the skin to form a breathable physical barrier, meanwhile, invasion of bacteria is effectively isolated, secondly, moisture of the skin can be supplemented through the moisturizing performance of the hydrogel dressing, and due to the flexibility and elasticity of the hydrogel dressing, the use comfort of the hydrogel dressing is further improved, and painless dressing change is achieved. More importantly, long-acting release of the zinc ions can inhibit bacterial infection at the wound, promote wound repair and accelerate wound healing.

An oral care composition with retinoid compound, amino acid, and / or olive oil. An oral care composition with retinoid compound, a pentapeptide, such as palmitoyl KTTKS [SEQ ID NO. 2], and / or an olive oil. Methods of preventing gingival recession, stopping gingival recession, reversing gingival recession, decrease gum recession, increase gingival barrier protection, promoting collagen synthesis, promoting extracellular matrix synthesis, increasing gum resilience, increasing epidermal thickness and / or promoting fibrillin synthesis, in an oral cavity of an animal by contacting the oral care compositions with at least one surface of the oral cavity.

Methods are disclosed for dissecting a mucosa and a submucosa from a muscularis propria from a region of an organ of a subject, wherein the organ is not the esophagus. In some embodiments, the organ is in the gastrointestinal tract. These methods include injecting submucosally into the organ of the subject a pharmaceutical composition comprising an extracellular matrix (ECM) hydrogel to form a cushion between the submucosa and the underlying muscularis propria at the region of the organ, wherein the ECM hydrogel has the following characteristics: a) a time to 50% gelation of less than 30 minutes at a temperature of about 37° C.; b) a flow viscosity suitable for infusion into the organ; and c) a stiffness of about 10 to about 400 Pascal (Pa).

The invention relates to the technical field of biomedical material modification, in particular to a sulfhydrylation biocompatible polymer derivative, a preparation method thereof, extracellular matrix imitating injectable in-situ cross-linked hydrogel and application thereof. According to the invention, a pre-acylation strategy is firstly carried out on the biocompatible polymer containing both amino and carboxyl, so that a three-dimensional cross-linked byproduct formed by coupling of self amido bonds is avoided, meanwhile, the number of side chain carboxyl for sulfhydrylation modification is increased, and the accurate and adjustable pre-acylation rate can optimize the physical and chemical properties of the derivative; meanwhile, a disulfide bond is used as a protective precursor, active sulfydryl is introduced through amido bond coupling, self-oxidation of the active sulfydryl in the reaction process is avoided, sulfydryl activity is guaranteed, hydrazine-containing reagents are avoided, safety is further improved, virus residues and immunological rejection risks are avoided, universality is high, and the application prospect is wide; and in combination with a high-concentration preparation method, the method has the advantage of large-scale production, and further widens the industrial application potential.

The invention discloses a dual-network injectable hydrogel as well as a preparation method and application thereof. The hydrogel is methacrylated gelatin / oxidized hyaluronic acid loaded with metal polyphenol nano-particles, and the metal polyphenol nano-particles are copper-gallic acid nano-particles which contain quaternized chitosan coatings and are loaded with antibacterial peptide HHC36. After the hydrogel is injected into a periodontal pocket, the hydrogel can adapt to different defect shapes, and the CuGA-coated HHC36-coated QCS nanoparticles are released by responding to a periodontitis microenvironment, so that the effects of resisting periodontal pathogenic bacteria, removing local excessive ROS (reactive oxygen species), effectively promoting fibroblast proliferation, collagen deposition, extracellular matrix accumulation and angiogenesis and further promoting periodontal bone regeneration are achieved. The preparation process is simple, the injectable hydrogel is low in price and easy to obtain, the injectable hydrogel can be prepared through simple steps, and a new thought is provided for treatment of periodontitis.

Composite matrix materials including a plurality of microgel particles having a mean particle size in a range of about 1 μm to about 100 μpm, and an interstitial matrix, where the composite matrix is characterized by reversible yield-stress behavior at 4°C; methods of preparation of the composite matrix materials; and methods of preparing organoids from the composite matrix materials are disclosed.

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The invention relates to the technical field of medical nano-materials, and discloses an iron-manganese bimetallic MOFs nano-enzyme hydrogel compound and a preparation method and application thereof, and the method comprises the following steps: step 1, preparing FeMn bimetallic MOFs nano-enzyme MIL-101 by adopting a microwave-assisted solvothermal method; step 2, preparing oxidized hyaluronic acid; step 3, synthesizing a hydrogel precursor protocatechuic aldehyde and a complex PA-Fe of iron ions; and step 4, fully mixing the quaternary ammonium salt chitosan solution with the PA-Fe solution, adding the oxidized hyaluronic acid solution and the MIL-101 powder under continuous magnetic stirring, and finally forming the iron-manganese bimetal MOFs nano-enzyme hydrogel compound. The delivery efficiency of the bimetallic nano-enzyme is improved, the in-vivo retention time of the bimetallic nano-enzyme is prolonged, the bimetallic nano-enzyme has antibacterial, hemostatic, adhesion and anti-oxidation effects, the steady state of an extracellular microenvironment of the intervertebral disc is recovered, an extracellular matrix of intervertebral disc tissue is remodeled, and the effect of promoting the repair and regeneration of the intervertebral disc by the nano-enzyme is synergistically enhanced.

A regenerative tissue repair product and method of making includes a decellularized extracellular matrix derived from animal liver. The decellularized extracellular matrix retains the collagen morphology of the animal liver prior to decellularization and having less than 500 ng DNA / mg dry extracellular matrix, greater than 760 microgram total collagen per milligram, the collagen comprising soluble and insoluble collagen, and greater than 1.6 μg glycosaminoglycans per milligram of dry extracellular matrix.

The invention relates to a decellularized acellular dermal tissue prepared by supercritical fluid extraction. More specifically, the present invention relates to a cell-free dermal tissue having improved extracellular matrix retention levels and optimized mechanical properties (e.g., critical loading and elastic restoring force) by extraction using supercritical fluids. The acellular dermal tissue of the present invention is obtained by decellularization, is not treated with a surfactant, thus retains mechanical properties, does not have toxicity caused by a residual surfactant, and minimizes transplant rejection, and thus can be effectively used for treating a patient with damaged skin tissue.

The invention belongs to the technical field of surgical article materials, and particularly relates to a collagen membrane with an effect of inducing ordered remodeling of tissues as well as a preparation method and application of the collagen membrane. The collagen membrane is obtained by performing hot pressing treatment on a fiber membrane; the fiber membrane is formed by crossing and laminating fibers with the diameter of 0.5-15 microns; the fiber comprises collagen and poly (L-lactide-caprolactone), and the mass ratio of the collagen to the poly (L-lactide-caprolactone) is 1: (1.5-2.5). The collagen membrane provided by the invention combines collagen with good biocompatibility and a tissue repair promoting effect with biodegradable PLCL with excellent mechanical properties and good cell adhesion, not only has an extracellular matrix-like structure, but also has good structural stability, is beneficial to regulation of cell behaviors and tissue regeneration, and can be used for preparing the collagen membrane. The growth, adhesion and proliferation of host cells can be effectively promoted, so that ordered remodeling of tissues is induced.

The invention discloses an anti-aging transdermal drug delivery microneedle preparation solution, a preparation method and application of the anti-aging transdermal drug delivery microneedle preparation solution and a transdermal drug delivery soluble microneedle, and belongs to the technical field of microneedle preparation. Comprising 0.25%-10% of an anti-sugar and anti-oxidation component, 0.1%-10% of an anti-ultraviolet damage component, 0.1%-10% of a cell and collagen regeneration component, 10%-30% of an extracellular matrix supplement component, 0.05%-3.1% of a nerve inhibition component and the balance of water. The transdermal drug delivery microneedle preparation solution disclosed by the invention can be used for preparing a transdermal drug delivery soluble microneedle, and the transdermal drug delivery soluble microneedle can be used for rapidly improving the intercellular capacity, so that the effects of improving the material transportation speed, the cell division and differentiation speed and the construction speed of extracellular matrixes such as collagen and the like are achieved; therefore, the purpose of recovering the tough and elastic young state of the skin is achieved.

The invention discloses polycaprolactone / acellular extracellular matrix composite hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological tissue engineering. The amino-modified polycaprolactone nanofiber and the fat acellular matrix are compounded in proportion for the first time, genipin is used as a cross-linking agent of the composite hydrogel, and the hydrogel of an injectable composite material is prepared. The crosslinking degree, the gelation time, the degradation performance, the swelling performance and the mechanical performance of the composite hydrogel with different proportions are studied, and the influence of the composite hydrogel on angiogenesis and fat regeneration in vivo is studied. According to the novel injectable composite hydrogel disclosed by the invention, the 1% PCL / DAT group composite hydrogel has good adipogenesis and vascularization effects, and a new thought is provided for subsequent soft tissue repair and regeneration research.

To provide a compound and the use thereof for the treatment for regeneration of connective tissue following the degeneration process of articular cartilage due to osteoarthritis, in the case of humans, the degeneration process of articular cartilage caused by excessive stress, for the treatment of skin aging, and for the prevention of skin cancer.SOLUTION: For example, an amino acid derivative of glucosamine having the following structure is provided.SELECTED DRAWING: None

1. Name of the Design Product: Packaging Box (Extracellular Matrix). 2. Use of the Design Product: For product outer packaging. 3. Design Key Points of the Design Product: The combination of shape and pattern. 4. Picture or Photograph Most Indicating the Design Key Points: 3D View of Design 1. 5. Design 1 is designated as the basic design.