118 results about "Extracellular ground substance" patented technology

The invention relates to a double-layer dressing for promoting wound healing and inhibiting scars and a preparation method and application thereof.The double-layer dressing comprises a hydrogel layer and a piezoelectric film layer compounded on the hydrogel layer, and the piezoelectric film layer is prepared by mixing an organic piezoelectric material, a ferroelectric material and an organic solvent and then conducting an electrostatic spinning technology; the hydrogel layer is formed by curing a methacrylic anhydride acellular extracellular matrix, methacrylic anhydride catechol chitosan and conductive nanoparticles under the action of a curing agent. The lower skin contact layer of the double-layer dressing is the hydrogel layer, so that the directional growth of fibroblasts is favorably induced; the upper dressing is a piezoelectric material composite ferroelectric material, is beneficial to piezoelectric stimulation re-induction function recovery after final skin repair and guidance of directional growth of wound cells, and has good skin wound induction repair and scar inhibition effects.

The invention relates to the field of biomedical application of inorganic materials, in particular to application of lanthanum carbonate in preparation of drugs for resisting liver cirrhosis and inhibiting liver inflammations, the drugs comprise lanthanum carbonate and pharmaceutically acceptable auxiliary materials, the drugs are ground and then mixed with food to prepare a drug mixture, and the drug mixture is prepared into the drug for resisting liver cirrhosis and inhibiting liver inflammations. The mass ratio of the lanthanum carbonate in the medicine mixture is 1-10%, and the mechanism of the lanthanum carbonate for treating the liver cirrhosis is as follows: interference or blocking of activation and proliferation of hepatic stellate cells, inhibition of extracellular matrix generation and promotion of extracellular matrix degradation. According to the invention, a rat liver cirrhosis model is constructed through chemical induction, and the effects of reversing liver cirrhosis and inhibiting liver inflammation are found when a rat is treated by taking lanthanum carbonate orally, so that a new candidate drug is provided for clinical treatment of liver cirrhosis.

The invention relates to the technical field of bone tissue materials, and discloses a metal-doped biomimetic mineralized dECM cryogel and a preparation method and application thereof.The preparation method of the cryogel comprises the steps that firstly, hyaluronic acid and methacrylic anhydride are mixed in a solvent, the pH value is adjusted, a reaction is conducted, and methacrylic acid hyaluronic acid is obtained; then under the condition of a photoinitiator, mixing the methacrylic acid hyaluronic acid with an acellular extracellular matrix, and performing freezing and crosslinking treatment to obtain the cryogel, and finally, soaking the cryogel in a mineralization solution containing Ca < 2 + > and / or Mg < 2 + >, adding a buffer solution containing phosphite ions, incubating, and washing to obtain the crystal gel. The biomimetic mineralized dECM cryogel prepared by the method disclosed by the invention is good in ion release stability, can significantly improve the migration ability of HUVECs, contributes to promoting proliferation of rMSCs and improving the osteogenic differentiation potential of the rMSCs, and can be used for regeneration and repair of bone defects.

The invention relates to the technical field of medicines, in particular to application of alpha-ketoglutaric acid in preparation of a medicine for preventing and / or treating myopia. The invention provides application of alpha-ketoglutaric acid or pharmaceutically acceptable salt thereof in preparation of products for preventing and / or treating myopia, and the alpha-ketoglutaric acid is taken as an important intermediate metabolite of tricarboxylic acid cycle in mitochondrial aerobic respiration and has oral safety; researches show that alpha-ketoglutaric acid can inhibit myopia progress and ocular axis elongation by improving sclera fibroblast phenotype transformation and extracellular matrix remodeling, and a new idea is provided for preparation of myopia intervention drugs.

The invention discloses hydrogel bio-ink, a preparation method of the hydrogel bio-ink, a 3D printing biodegradable airway stent and application of the 3D printing biodegradable airway stent. The preparation method comprises the following steps that S1, lung tissue is subjected to freeze thawing, slicing, sequential decellularization of SDS and TritonX-100, air drying, smashing and cryopreservation, and a lung extracellular matrix is obtained; s2, performing sterilization and pepsin treatment on the lung extracellular matrix prepared in S1 to obtain a pretreated lung extracellular matrix; and S3, mixing methyl propionylated gelatin and an alginate solution, pouring the mixture into solid gel, gelatinizing the solid gel, and adding the pretreated lung extracellular matrix to prepare the hydrogel bio-ink. The prepared pig lung acellular extracellular matrix does not have an obvious nuclear structure, and DNA (Deoxyribonucleic Acid) determination shows that the concentration of an acellular group is obviously reduced (P is less than 0.01); along with the increase of the dECM concentration, the porosity is reduced, and the degradation time is prolonged; the airway stent is printed in a 3D biological printing mode, no obvious cytotoxicity (P is larger than 0.05) exists in a cytotoxicity experiment, and the mechanical property part of the airway stent is similar to that of a silicone stent.

An oral care composition with retinoid compound, amino acid, and / or olive oil. An oral care composition with retinoid compound, a pentapeptide, such as palmitoyl KTTKS [SEQ ID NO. 2], and / or an olive oil. Methods of preventing gingival recession, stopping gingival recession, reversing gingival recession, decrease gum recession, increase gingival barrier protection, promoting collagen synthesis, promoting extracellular matrix synthesis, increasing gum resilience, increasing epidermal thickness and / or promoting fibrillin synthesis, in an oral cavity of an animal by contacting the oral care compositions with at least one surface of the oral cavity.

The invention relates to the technical field of biomedical material modification, in particular to a sulfhydrylation biocompatible polymer derivative, a preparation method thereof, extracellular matrix imitating injectable in-situ cross-linked hydrogel and application thereof. According to the invention, a pre-acylation strategy is firstly carried out on the biocompatible polymer containing both amino and carboxyl, so that a three-dimensional cross-linked byproduct formed by coupling of self amido bonds is avoided, meanwhile, the number of side chain carboxyl for sulfhydrylation modification is increased, and the accurate and adjustable pre-acylation rate can optimize the physical and chemical properties of the derivative; meanwhile, a disulfide bond is used as a protective precursor, active sulfydryl is introduced through amido bond coupling, self-oxidation of the active sulfydryl in the reaction process is avoided, sulfydryl activity is guaranteed, hydrazine-containing reagents are avoided, safety is further improved, virus residues and immunological rejection risks are avoided, universality is high, and the application prospect is wide; and in combination with a high-concentration preparation method, the method has the advantage of large-scale production, and further widens the industrial application potential.

The invention discloses a dual-network injectable hydrogel as well as a preparation method and application thereof. The hydrogel is methacrylated gelatin / oxidized hyaluronic acid loaded with metal polyphenol nano-particles, and the metal polyphenol nano-particles are copper-gallic acid nano-particles which contain quaternized chitosan coatings and are loaded with antibacterial peptide HHC36. After the hydrogel is injected into a periodontal pocket, the hydrogel can adapt to different defect shapes, and the CuGA-coated HHC36-coated QCS nanoparticles are released by responding to a periodontitis microenvironment, so that the effects of resisting periodontal pathogenic bacteria, removing local excessive ROS (reactive oxygen species), effectively promoting fibroblast proliferation, collagen deposition, extracellular matrix accumulation and angiogenesis and further promoting periodontal bone regeneration are achieved. The preparation process is simple, the injectable hydrogel is low in price and easy to obtain, the injectable hydrogel can be prepared through simple steps, and a new thought is provided for treatment of periodontitis.

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The invention relates to the technical field of medical nano-materials, and discloses an iron-manganese bimetallic MOFs nano-enzyme hydrogel compound and a preparation method and application thereof, and the method comprises the following steps: step 1, preparing FeMn bimetallic MOFs nano-enzyme MIL-101 by adopting a microwave-assisted solvothermal method; step 2, preparing oxidized hyaluronic acid; step 3, synthesizing a hydrogel precursor protocatechuic aldehyde and a complex PA-Fe of iron ions; and step 4, fully mixing the quaternary ammonium salt chitosan solution with the PA-Fe solution, adding the oxidized hyaluronic acid solution and the MIL-101 powder under continuous magnetic stirring, and finally forming the iron-manganese bimetal MOFs nano-enzyme hydrogel compound. The delivery efficiency of the bimetallic nano-enzyme is improved, the in-vivo retention time of the bimetallic nano-enzyme is prolonged, the bimetallic nano-enzyme has antibacterial, hemostatic, adhesion and anti-oxidation effects, the steady state of an extracellular microenvironment of the intervertebral disc is recovered, an extracellular matrix of intervertebral disc tissue is remodeled, and the effect of promoting the repair and regeneration of the intervertebral disc by the nano-enzyme is synergistically enhanced.

To provide a compound and the use thereof for the treatment for regeneration of connective tissue following the degeneration process of articular cartilage due to osteoarthritis, in the case of humans, the degeneration process of articular cartilage caused by excessive stress, for the treatment of skin aging, and for the prevention of skin cancer.SOLUTION: For example, an amino acid derivative of glucosamine having the following structure is provided.SELECTED DRAWING: None

The application discloses a kind of high adhesion enzymatic hydrogel for promoting vascular intimal repair and a preparation method thereof, and belongs to the technical field of hydrogel preparation.The preparation method is as follows: S1, using methacrylic anhydride to modify the double bond of hyaluronic acid, then crosslinking with dopamine to obtain dopamine-modified double-bonded hyaluronic acid;S2, after removing oxygen from the hyaluronic acid solution, add a crosslinking agent and tyramine hydrochloride, and react in an acidic environment to obtain tyramine-modified hyaluronic acid;S3, mix the dopamine-modified double-bonded hyaluronic acid, tyramine-modified hyaluronic acid and deionized water to obtain a hydrogel precursor solution;S4, then use enzymatic reaction to obtain the hydrogel.The method designed by the application is simple, does not require additional light curing equipment, and the constructed hydrogel is similar to the extracellular matrix.The hydrogel can be coated into different shapes to adapt to different environments.The hydrogel is formed by enzymatic reaction, which is rapid, well adhered to the tissue, biocompatible, and suitable for application in vascular repair.

One embodiment of the present invention provides an extracellular matrix-based hybrid ink for 3D printing, which comprises an extracellular matrix having an amine group and a modified hyaluronic acid having an ethylenically unsaturated bond functional group introduced therein, characterized in that the extracellular matrix and the modified hyaluronic acid are in a crosslinked state due to a chemical bond formed between at least a portion of the amine groups present in the extracellular matrix and the ethylenically unsaturated bond functional group present in the modified hyaluronic acid. The hybrid ink according to one embodiment of the present invention has mechanical properties suitable for 3D printing due to the chemical crosslinking between the components, the extracellular matrix and the modified hyaluronic acid, and can control the drug release rate by adjusting the chemical crosslinking density.

The invention relates to the technical field of biomedical materials and tissue engineering, and discloses a preparation method and application of adjustable porous fat foam. Comprising the following steps: fat tissue pretreatment and freeze-thaw circulation, multi-step combined decellularization and impurity removal, graded washing and purification, freeze grinding and freeze drying, preparation of a decellularized fat matrix suspension, porous foam scaffold forming and foam scaffold gradient rehydration. By adopting a multi-step combined decellularization and impurity removal process, residual lipid droplets in adipose tissues can be actively extruded out by mechanical squeezing assisted by isopropanol degreasing, a lipid wrapping phenomenon is broken, meanwhile, residual nucleic acid and adipose components can be thoroughly removed by virtue of a synergistic degradation effect of nuclease and lipase, the immunogenicity risk is reduced, and the immunogenicity of the adipose tissues is improved. All the steps have a synergistic effect under strict control of temperature, concentration and time parameters, so that the structural integrity of the extracellular matrix is ensured, the decellularization efficiency and thoroughness are improved, and the biological safety and biocompatibility of a final product are ensured.

Methods are disclosed for dissecting a mucosa and a submucosa from a muscularis propria from a region of an organ of a subject, wherein the organ is not the esophagus. In some embodiments, the organ is in the gastrointestinal tract. These methods include injecting submucosally into the organ of the subject a pharmaceutical composition comprising an extracellular matrix (ECM) hydrogel to form a cushion between the submucosa and the underlying muscularis propria at the region of the organ, wherein the ECM hydrogel has the following characteristics: a) a time to 50% gelation of less than 30 minutes at a temperature of about 37° C.; b) a flow viscosity suitable for infusion into the organ; and c) a stiffness of about 10 to about 400 Pascal (Pa).

The invention relates to the technical field of medical instruments for the ophthalmology department, and particularly discloses a preparation method of a biological membrane strip for sclera reinforcement after the ophthalmology department, the preparation method comprises multilayer stacking and edge folding fixing, and specifically comprises the steps that pretreated extracellular matrixes are stacked in multiple layers and placed in a mold, and then four corners are folded inwards to form a fusiform shape; then applying pressure to fix by using upper and lower plane clamping plates, wherein the pressure is 10-200kpa; drying with a mold to obtain a biofilm strip; the middle section of the biological membrane strip is widest and gradually narrows towards two ends in an arc-shaped transition manner; the total length of the biological membrane strip is 20-70mm, and the thickness of the biological membrane strip is 0.1-0.4 mm; the width of the middle section is 10-40 mm, and the length of the middle section is 10-60 mm; and the width of the two ends is 5-20mm. The edge mechanical strength of the prepared biofilm strip is superior to that of the middle section, and the biofilm strip can better meet the requirements of posterior sclera reinforcement surgery for material reliability and long-term stability.

The invention belongs to the technical field of biomedical materials, and discloses an anisotropic nanofiber yarn composite scaffold as well as a preparation method and application thereof. The anisotropic nanofiber yarn composite scaffold is formed by compounding methylacryloyl modified gelatin and a nanofiber yarn scaffold prepared through electrostatic spinning. The anisotropic nanofiber yarn composite scaffold not only has more excellent mechanical properties, but also can provide a more suitable microenvironment for cell adhesion and growth, can significantly improve the remodeling efficiency of an extracellular matrix, and has a wide application prospect in the field of heart valve repair.

The application discloses a synthesis method of a composite gel based on in-situ mineralized bone-like hydroxyapatite, which comprises the following steps: taking animal periosteum, and sequentially treating the animal periosteum with an immunostaining permeating liquid and a lysis liquid; sterilizing, detoxifying and washing the treated periosteum tissue, and then freeze-drying the periosteum tissue; grinding the periosteum tissue into powder, and then digesting the powder in a solution to obtain extracellular matrix; dissolving calcium chloride in ethanol, adding triethylamine under stirring, then adding an ethanol solution of phosphoric acid under stirring, centrifuging to discard the supernatant, and washing and resuspending the calcium phosphate mineralization precursor with ethanol to obtain the calcium phosphate mineralization precursor; uniformly mixing the calcium phosphate mineralization precursor with the extracellular matrix to obtain a gel, and in-situ biomimetic mineralizing the gel to obtain bone-like hydroxyapatite, which is used as the composite gel. The application further provides the composite gel prepared by the above synthesis method and application of the composite gel in preparation of a bone defect repair product. The composite gel has excellent biocompatibility, bone conduction, bone induction, blood vessel formation capacity and mechanical performance in vivo and in vitro.

The invention belongs to the technical field of tissue engineering, and particularly relates to a bio-ink system for osteochondral repair as well as a preparation method and application of the bio-ink system. The system comprises first bio-ink and second bio-ink which are respectively used for simulating cartilage and bone tissue. The first biological ink is prepared from methacrylic acid gelatin, acellular extracellular matrix powder, aldehyde modified natural polysaccharide, a photoinitiator and a transforming growth factor; the second biological ink is prepared from methacrylic acid gelatin, acellular extracellular matrix powder, nano-hydroxyapatite, aldehyde modified natural polysaccharide, a photoinitiator and human bone morphogenetic protein BMP-2. The bio-ink system can guarantee smooth extrusion and interlayer fusion in the printing process; the constructed bionic microenvironment and the affinity effect of the bionic microenvironment on growth factors are suitable for 3D printing construction of the osteochondral integrated bionic scaffold and have good clinical transformation potential.

The invention discloses a composite multi-oligopeptide composition for skin repair and an application of the composite multi-oligopeptide composition. The multi-oligopeptide composition comprises the following components in parts by weight: 1 to 5 parts of acetyl hexapeptide-8, 0.1 to 1.5 parts of acetyl tetrapeptide-3, 1 to 20 parts of acetyl tetrapeptide-9, 0.1 to 1.5 parts of acetyl tetrapeptide-11, 10 to 190 parts of carnosine, 10 to 100 parts of decarboxylated carnosine hydrochloride, 10 to 120 parts of tripeptide-1, 1 to 50 parts of tetrapeptide-4, 1 to 20 parts of hexapeptide-9, 1 to 10 parts of palmitoyl pentapeptide-4, 1 to 5 parts of palmitoyl tetrapeptide-7, 1 to 10 parts of palmitoyl tripeptide-1, 1 to 35 parts of palmitoyl tripeptide-8, 0.1 to 2.5 parts of oligopeptide-1, 0.1 to 0.5 part of oligopeptide-2 and 0.1 part of carnosine. And 1 part of oligopeptide-5. The multi-oligopeptide composition has excellent anti-wrinkle and anti-aging effects, has the effects of promoting tissue repair and wound healing, strengthening skin barrier and resisting inflammation and relieving, is applied to skin medicine and skin beauty, and has the effects of promoting regeneration and remodeling of an extracellular matrix (ECM), regulating inflammation, blocking inflammatory aging and strengthening a dermis-epidermis joint (DEJ).

The invention provides a small-molecule compound A3 with a good treatment effect on metabolism-related fatty liver diseases, the small-molecule compound A3 is innovatively designed and synthesized based on a pyrene skeleton, and the application of the small-molecule compound A3 in drugs for preventing and / or treating the metabolism-related fatty liver diseases. By establishing a standard in-vivo and in-vitro pharmacodynamic evaluation model, it is proved that the composition has a remarkable intervention effect on the metabolism-related fatty liver diseases. The compound improves the core pathological process of the metabolism-related fatty liver disease through multi-mechanism synergism, can effectively regulate abnormal lipid metabolism, inhibits hepatocyte vacuolation lesion and slows down excessive deposition of extracellular matrix, and shows the advantage of multi-channel treatment.

The invention provides a preparation method of acellular extracellular matrix nanofiber, which comprises the following steps: selecting fresh biological tissue, scraping with a blade to remove fat, cleaning with distilled water, and cutting the cleaned biological tissue into small pieces for later use; putting the diced biological tissue into a phosphate buffer solution for treatment, then treating the diced biological tissue with a TritonX-100 solution, cleaning the decellularized biological tissue with ultrapure water, finally freeze-drying the biological tissue and crushing the biological tissue with a grinding machine to prepare a decellularized extracellular matrix, and preserving the decellularized extracellular matrix at low temperature for later use; the preparation method comprises the following steps: taking a proper amount of an extracellular matrix and lithium hydroxide, adding the extracellular matrix and lithium hydroxide into dimethyl sulfoxide, then carrying out rapid magnetic stirring, gradually dissolving and permeating lithium hydroxide into the extracellular matrix in the stirring process so as to swell the extracellular matrix, gradually disintegrating the swelled extracellular matrix along with the increase of the stirring time so as to form nanofibers, then dispersing into dimethyl sulfoxide to form a faint yellow turbid liquid; and dialyzing the obtained faint yellow suspension in water, and removing lithium hydroxide and dimethyl sulfoxide to obtain the acellular extracellular matrix nanofiber dispersion liquid.

The invention belongs to the technical field of biomedical materials, and particularly discloses chitosan / polydopamine / copper sulfide composite hydrogel as well as a preparation method and application thereof. According to the hydrogel, through the synergistic effect of the chitosan, the polydopamine and the copper sulfide nanoparticles, a multifunctional composite system with rapid hemostasis, photo-thermal antibiosis, shape adaptability and biocompatibility is constructed. Chitosan promotes platelet aggregation and thrombin generation, polydopamine enhances adhesiveness and simulates an extracellular matrix, copper sulphide endows photo-thermal response ability, and heat energy can be efficiently converted to kill bacteria under near-infrared light irradiation. The preparation method comprises the following steps: firstly preparing polydopamine, then synthesizing copper sulfide nanoparticles, and then obtaining the composite hydrogel. The hydrogel has excellent injectability, self-repairability and conductivity, the hemolysis rate is smaller than 5%, the blood coagulation index is remarkably increased, the hydrogel is suitable for treatment of body surface wounds, deep tissue bleeding and infectious wounds, and a low-cost and efficient solution is provided for clinical hemostasis.

The invention relates to an anti-wrinkle whitening composition and a preparation method thereof, and belongs to the technical field of cosmetics, the composition comprises the following raw materials: composite peptide, hydroxypropyl tetrahydropyrantriol, a plant extract, vegetable oil, an emulsifier, a preservative and a solvent. According to the application, acetyl hexapeptide-7 and tripeptide-10 citrulline are selected as composite peptides, and the composite peptides, the Xixia lotus bulb extract and the hydroxypropyl tetrahydropyrantriol are compounded to serve as main anti-wrinkle components, so that different dimensions of reducing dynamic lines, increasing the collagen content and increasing the extracellular matrix content so as to assist in enhancing collagen deposition can be started; due to the synergistic effect, the finished product of the composition has an excellent anti-wrinkle effect; in addition, a patrinia scabiosaefolia extract, a pepperweed root extract, grape seed oil and a physalis alkekengi extract are combined for use, and the outstanding whitening effect is synergistically exerted from different dimensions of inhibiting melanocyte activity, reducing melanogenesis and reducing pigmentation.

The application relates to an electrostatic spinning anti-adhesion membrane and a preparation method and application thereof. The electrostatic spinning anti-adhesion membrane comprises an inner layer, an intermediate layer and an outer layer which are sequentially stacked; the inner layer comprises polyglycolide-lactide-polyethylene glycol, the intermediate layer comprises polyglycolide-lactide, and the outer layer comprises polylactic acid-caprolactone. The electrostatic spinning anti-adhesion membrane has a three-dimensional nanofiber network structure of a biomimetic extracellular matrix, excellent mechanical properties, body temperature response softening and gradient degradation characteristics, can balance the degradation speed and the mechanical properties, reduce the mechanical damage of the material to the surrounding tissues, and can effectively prevent postoperative tissue adhesion.

The application provides a red light excitable photoelectric response heterostructure nanotube, a hydrogel and a preparation method and application thereof, and belongs to the technical field of nanomaterials. By integrating a heterostructure, a quantum confinement effect and morphological modification, a one-dimensional TiO2 / Bi2S3 quantum dot nanotube with visible red light excitation and high photoelectric conversion efficiency is prepared, which is combined with a hydrogel precursor to simulate an extracellular matrix, so that a high-performance red light response photoelectric hydrogel dressing is developed. The hydrogel dressing has excellent ability in promoting nerve ending reinnervation, promoting skin substructure repair and regulating immune response in a deep burn model, and has wide application prospects in overall skin repair.

The invention discloses application of water microspheres of modified polysaccharide in promoting wound skin regeneration and inhibiting wound skin fibrosis. In the invention, compared with a Control group (silicone oil) as a control and an unmodified polysaccharide water microsphere group (AVBEC), when the modified polysaccharide water microsphere group (AVBER) is externally applied to the skin of a bama miniature pig in a deep II-level scald model, the wound healing can be effectively promoted, the inflammation degree and duration can be controlled, and the extracellular matrix composition and plasticity can be regulated; expression of fibrosis marker protein is reduced, and the dual effects of promoting skin regeneration and inhibiting fibrosis are achieved. The problems that natural extracts and common polysaccharide microsphere products are low in biological activity and poor in stability are solved, and new functions of modified polysaccharide are found.

The invention relates to the technical field of livestock breeding and animal nutrition, in particular to fattening pig feed and application thereof.The fattening pig feed is prepared from, by weight, 50 parts of corn flour, 19-21 parts of concentrated feed, 9-11 parts of wheat bran and 20 parts of wild buckwheat straw powder. According to the formula, the wild buckwheat straw is used as a functional fiber source, through scientific matching and synergistic effect with conventional energy raw materials, the micro-ecological structure of the intestinal tract of the fattening pig is effectively optimized, enrichment of beneficial microbial communities such as lactobacilli in the phylum firmicalis is promoted, and therefore the steady state of the intestinal tract can be maintained. Meanwhile, in the liver of the fattening pigs fed with the formula, expression of various genes related to extracellular matrix structures and tissue homeostasis is up-regulated, and support is provided for tissue structure maintenance and healthy growth. The method provides a feasible path for resource utilization of wild buckwheat straws, and has double benefits of breeding health promotion and agricultural byproduct value increase.

The invention discloses a multi-gradient step-by-step degradation micro-nanofiber artificial tendon scaffold which is of a multi-layer skin-core structure which is formed by winding and coating a plurality of layers of nanofiber membranes layer by layer and is provided with a core layer and a plurality of skin layers, the plurality of layers of nanofiber membranes are synthetic polymer / natural polymer blended micro-nanofiber membranes with different proportions, and the synthetic polymer / natural polymer blended micro-nanofiber membranes are obtained by performing an electrostatic spinning process on a blended spinning solution formed by mixing a synthetic polymer and a natural polymer according to different proportions; the winding and coating rule of the synthetic polymer / natural polymer blended micro-nano fiber membrane in the multi-layer skin-core structure is that the proportion of the synthetic polymer is gradually reduced from the core layer to each skin layer. The method not only can truly simulate an extracellular matrix and provide a favorable environment for cell repair and regeneration, but also can provide continuous mechanical support for regenerated tissues in a long period of tendon repair, and is suitable for industrial production and worthy of popularization and application.

The invention relates to the field of medical implant materials, and particularly discloses an absorbable patch for uterine muscular layer repair and a preparation method of the absorbable patch. The invention discloses an absorbable patch for repairing a uterine muscular layer. The absorbable patch is prepared by mixing 2-9.5 parts of poly (L-lactide-caprolactone) and 0.5-8 parts of a biomedical material, the molecular weight of the poly (L-lactide-caprolactone) is 5 to 30 thousand; the preparation method comprises the following steps: mixing a biomedical material stock solution with a poly (L-lactide-caprolactone) stock solution, performing electrostatic spinning, freeze drying or tape casting treatment, and drying to remove an organic solvent, thereby obtaining the absorbable patch for uterine muscular layer repair. The absorbable patch disclosed by the invention not only can be completely degraded in vivo, but also has a similar extracellular matrix structure, can provide muscle cell repair for an injured part of a uterine muscular layer, avoids scar tissues formed by fibrosis, and reduces the risk caused by a secondary operation required by a uterine scar diverticulum.