14 results about "Extracellular ground substance" patented technology

The application provides a red light excitable photoelectric response heterostructure nanotube, a hydrogel and a preparation method and application thereof, and belongs to the technical field of nanomaterials. By integrating a heterostructure, a quantum confinement effect and morphological modification, a one-dimensional TiO2 / Bi2S3 quantum dot nanotube with visible red light excitation and high photoelectric conversion efficiency is prepared, which is combined with a hydrogel precursor to simulate an extracellular matrix, so that a high-performance red light response photoelectric hydrogel dressing is developed. The hydrogel dressing has excellent ability in promoting nerve ending reinnervation, promoting skin substructure repair and regulating immune response in a deep burn model, and has wide application prospects in overall skin repair.

The application belongs to the technical field of new materials, and discloses a transdermal drug delivery device and a preparation method and use method thereof. The transdermal drug delivery device comprises a flexible patch and a power supply. The flexible patch comprises a flexible substrate and a liquid metal electrode. The liquid metal electrode is embedded in the flexible substrate, and the flexible patch is modified by extracellular matrix proteins. The power supply is connected with the flexible patch. The use method is as follows: a drug is coated on the surface of skin, the transdermal drug delivery device is covered on the skin surface coated with the drug, the power supply is turned on, and the transdermal drug delivery device realizes the penetration of the drug into the skin through iontophoresis. The device simply connects the flexible patch with the power supply, realizes the miniaturization and portability of iontophoresis therapy, does not harm the skin, is efficient and safe, and has high patient compliance.

This invention relates to a biomimetic extracellular matrix hydrogel, its preparation method, and its application. The preparation method of this invention includes the following steps: (1) dissolving a natural high-molecular-weight polysaccharide in solvent A, then adding a halogenated oxyacid salt, and reacting in the dark; after the reaction is completed, adding ethylene glycol to terminate the reaction, dialyzing, and then freeze-drying to obtain GM-CHO powder; (2) dissolving the GM-CHO powder in solvent B, adding an amino acid polymer, and stirring at a constant temperature; then dialyzing and freeze-drying sequentially to obtain GMI glycopeptide; (3) dissolving the GMI glycopeptide and aminated glucomannan in solvent C, vortexing, and finally allowing it to stand at room temperature to form a biomimetic extracellular matrix hydrogel. The biomimetic extracellular matrix hydrogel of this invention can simultaneously achieve "correcting metabolic disorders, clearing pathogens, and simulating extracellular matrix", improving the retention time at the wound site and facilitating long-term therapeutic effects.

This invention discloses a biomimetic extracellular matrix composition, its preparation method, application, and cosmetics, belonging to the field of cosmetic technology. The composition comprises, by weight percentage, the following components: Type I collagen: 0.01%-0.5%, Type III collagen: 0.01%-0.3%, Type VII collagen: 0.005%-0.2%, Type XVII collagen: 0.005%-0.2%, elastin: 0.01%-0.3%, fibronectin: 0.005%-0.2%, moisturizing ingredients: 0.2%-2.0%, repair factors: 0.061%-0.8%, polypeptide ingredients: 0.02%-0.8%, β-Xylane: 0.5%-3.0%, excipients: 0%-5%, and the balance being water. This invention uses four types of collagen, elastin, and fibronectin as the core structure, and combines them with moisturizing ingredients, repair factors, and peptides to form a complete synergistic system. This system forms a multi-pathway, multi-level synergistic enhancement system that can biomimetically reconstruct the extracellular matrix structure of the entire skin layer, achieving six effects in one: anti-aging, firming, wrinkle reduction, repair, moisturizing, and brightening.

This invention provides a camellia seed diglyceride microcapsule, its preparation method, and its application, belonging to the field of cosmetic raw material preparation technology. The invention uses a three-dimensional network cross-linked polymer of sea fennel extract, BOXER, and recombinant collagen as the wall material. Recombinant collagen provides the macromolecular framework, and BOXER can cross-link with the recombinant collagen, optimizing the three-dimensional network structure of the wall material and filling the cross-linking sites. The polyphenols contained in the sea fennel extract participate in hydrogen bonding / covalent cross-linking, forming a dense three-dimensional network structure, increasing the mechanical strength of the wall material and encapsulating the camellia seed diglyceride core material, thus improving the stability and antioxidant properties of the camellia seed diglyceride. Sea fennel extract has excellent repair and anti-aging effects, promoting skin cell regeneration; BOXER can promote the synthesis of extracellular matrix glycosaminoglycans, strengthening skin firmness; and recombinant collagen can replenish lost collagen in the skin, improve skin elasticity, and enhance the anti-aging performance of the camellia seed diglyceride microcapsules.

This invention relates to the fields of biomedical technology and pharmaceutical formulations, and discloses a sustained-release powder spray for oral ulcers based on milk exosomes and its preparation method. The spray uses milk exosomes as the active ingredient, combined with an extracellular matrix-simulated network composed of sodium hyaluronate and collagen, and the stabilizer trehalose. It is prepared into a dry powder with a particle size of 2-15 μm through a microdroplet freeze-drying process. During preparation, the components are mixed and formed into microdroplets using a microfluidic device, then instantaneously frozen in liquid nitrogen at -80°C, and finally freeze-dried to obtain the powder. This formulation can be stably stored for a long time at 2-8°C. When used, it is sprayed onto the ulcer surface using a quantitative spray device. Upon contact with moisture, the gel network is reconstructed in situ, achieving sustained release and protection of the exosomes. It has the advantages of convenient use, accurate dosage, and long-lasting healing promotion.

The application discloses a 3D printing high-strength supramolecular hydrogel and application thereof in bone defect repair. The high-strength supramolecular hydrogel is formed by hydrogen bond, ion interaction and hydrophobic association non-covalent cross-linking of a positively charged high-molecular polymer, a negatively charged functional monomer and a photoinitiator, and has a dynamic network structure. The hydrogel has a structure similar to an extracellular matrix, has self-healing, viscoelasticity and dynamics, and is beneficial to cell adhesion, proliferation, migration and differentiation. The hydrogel has superfast gelation, realizes second-level gelation solidification, and can accurately 3D print a microporous structure due to triple forces of strong hydrogen bond, electrostatic interaction and hydrophobic interaction. The hydrogel has good biodegradability, and the degradation rate can be designed by adjusting ingredients and cross-linking density, so that the degradation rate is matched with the speed of new bone formation, and finally, the scaffold material is completely replaced by new bone tissue.

To provide a colored, fragmented extracellular matrix component capable of forming a structure with high visibility and suppressed color transfer. [Solution] A colored fragmented extracellular matrix component comprising a fragmented extracellular matrix component and a coloring agent adsorbed to the fragmented extracellular matrix component, wherein the coloring agent comprises at least one selected from the group consisting of water-insoluble and ethanol-soluble dyes and lipid-soluble dyes.

This invention discloses a preparation process for Poria cocos extracellular vesicle hydrogel for the treatment of androgenetic alopecia. By introducing SA, CMC-Na, and L-HA to construct an interpenetrating network hydrogel, it not only possesses excellent mechanical strength and anti-degradation ability but also effectively simulates the extracellular matrix environment. This invention significantly improves the structural stability of PCEVs through the loading of the interpenetrating network hydrogel, preventing premature inactivation during application. Simultaneously, leveraging the natural "medicinal and edible homology" properties of the raw materials, it ensures extremely high biosafety of the formulation, with no irritation or immunogenicity risks. This invention, through the synergistic effect of the "carrier-drug" combination, provides an innovative treatment solution for androgenetic alopecia with significant clinical application prospects.

This invention discloses a nanodelivery carrier composite extracellular matrix microsphere, its preparation method, and its applications. The method involves preparing microspheres from extracellular matrix and a photoinitiator, forming ice crystal templates through freezing, followed by photocrosslinking at low temperatures. After removing the ice crystals by heating, a second crosslinking process is performed to construct microspheres with a three-dimensional interconnected nanoporous structure. These microspheres are co-incubated with a nanodelivery carrier loaded with active ingredients, utilizing the salt charge shielding effect to induce aggregation and physically trap the nanodelivery carrier within the pores. The microspheres possess a high specific surface area and spatially confined structure, improving the loading efficiency of the nanodelivery carrier and maintaining particle size and structural stability during storage and reconstitution. The nanodelivery carrier enables controlled release of active ingredients, while the extracellular matrix itself participates in the regulation of the hair follicle microenvironment, forming a dual mechanism with the nanodelivery system to synergistically promote hair growth, making it suitable for hair loss treatment.

A method for obtaining decellularized extracellular matrix of the pancreas, that it comprises the following steps, in which: the pancreas is incubated in a disinfecting solution, advantageously containing betadine at a concentration of 2% (v / v), then in a solution with an antimicrobial agent, advantageously streptomycin at a concentration of 0.01% (w / v), and then frozen; the pancreas, warmed to a temperature of 4°C ± 2°C, is subjected to homogenization in a PBS solution and 5 mM EDTA-Na2 in a 3: 1 ratio, and the homogenized material is centrifuged, after which the hydrophobic fat phase is removed; the material is suspended in a solution of NaCl at a concentration of 0.5 M + 5 mM EDTA-Na2 (pH = 7.4), and then subjected to mixing, after which the material is filtered; the material is immersed in a PBS solution and incubated, then sieved, and the sieved material is mechanically minced; the material is immersed in a PBS solution with 0.01% (w / v) streptomycin and incubated, then sieved, and the sieved material is mechanically minced; the material is immersed in a PBS solution with 0.01% (w / v) streptomycin and incubated for 2 hours at 4°C ± 2°C, the step being repeated at least twice, and the sieved material is mechanically minced after each filtration; the obtained extracellular matrix is sieved, frozen, and then subjected to lyophilization and mechanical milling.

The utility model discloses a kind of skin cell regeneration beauty instrument based on multiple technologies, including base, support seat, placement plate, plasma probe and several ultrasonic probes;Support seat is connected with base, support seat and base interior are opposite, and placement plate is connected with support seat;Plasma probe one end is placed on placement plate, and several ultrasonic probe one end is placed on placement plate, and plasma probe and several ultrasonic probe are sequentially arranged and placed on placement plate;Plasma releases a large number of charged particles and free radicals to make skin surface bacteria lose activity, while let skin obtain the penetration of charged free radicals, it is favorable to promote the penetration ability after using multiple high-frequency ultrasound and cosmetics;1 / 3MHz, 3 / 10MHz and so on Dual-frequency probe of ultrasonic wave mixture, using different double ultrasonic wavelength technology, so that high-density ultrasonic wave can penetrate target area, let the extracellular matrix in effective area occur homochromatic vibration, save the expenditure of consumer, enhance the use effect of beauty instrument.

The application discloses a high-strength extracellular matrix film and a preparation method and application thereof, and belongs to the field of biomedical materials. The application provides a preparation method of the high-strength extracellular matrix film, which comprises the following steps: decellularization treatment and freeze-drying, grinding the freeze-dried decellularized pig organs into powder and adding the powder into a pepsin-hydrochloric acid solution for digestion, forming a pre-gel, forming an extracellular matrix hydrogel, obtaining an extracellular matrix film, obtaining a high-strength extracellular matrix film wet film, and obtaining a high-strength extracellular matrix film. The extracellular matrix film prepared by the foregoing method has the characteristics of high strength, and the strength far exceeds that of the currently reported natural polymer gel film, and even exceeds that of most synthetic high-molecular polymer gel films and composite films added with a crosslinking agent.