Preparation method of nano-fibrous silk fibroin-based porous scaffold

A technology of silk fibroin and nanofibers, applied in medical science, prostheses, etc., can solve the problems of lack of nanofiber structure, limitations of tissue engineering scaffold materials for tissue repair, difficulty in forming porous structures, etc., and achieve the goal of improving interaction degree, prolonging the resting time, and improving biocompatibility

Inactive Publication Date: 2011-04-13
SUZHOU UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, researchers have studied a variety of methods for preparing porous silk fibroin scaffolds, including freeze-drying, phase separation, salting-out, and electrospinning, but each of the above methods has its own insurmountable shortcomings.
For example, the pore size of the silk fibroin scaffold prepared by the salting-out method is generally above 400 microns, while the pore size of the porous material prepared by the electrospinning method is generally below 100 microns. limit
Freeze-drying method can prepare porous scaffolds with a large pore size range, but in the freezing process, silk fibroin is easy to self-assemble into a sheet-like structure, and it is difficult to form a well-structured porous structure
Moreover, the silk fibroin-based porous scaffolds prepared by all the above methods do not have the nanofibrous structure similar to the extracellular matrix, and the tissue compatibility still needs to be improved.

Method used

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  • Preparation method of nano-fibrous silk fibroin-based porous scaffold

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Embodiment 1

[0033] The preparation steps of the silk fibroin porous scaffold provided in this embodiment are as follows:

[0034] 1) Add 5 grams of silkworm cocoons into 2 liters of 0.5% sodium carbonate solution, treat at 98-100°C for 40 minutes, remove the external sericin, and obtain pure silk fibroin after washing thoroughly. The dried silk fibroin is dissolved in a 9.3 mol / liter lithium bromide solution at about 60° C. to obtain a silk fibroin mixed solution.

[0035] 2) Add the silk fibroin mixed solution into the cellulose dialysis membrane, and perform dialysis with deionized water to remove lithium bromide to obtain a pure silk fibroin aqueous solution.

[0036] 3) Adjust the concentration of silk fibroin to 1%, and place it at 4°C for 1 hour to stabilize the temperature of the solution at 4°C.

[0037]4) Dilute the collagen solution to 0.075%, and place it at 4°C for 1 hour to stabilize the solution temperature at 4°C.

[0038] 5) Blend the same volume of silk fibroin solution...

Embodiment 2

[0045] Add 5 grams of silkworm cocoons into 2 liters of sodium carbonate solution with a mass concentration of 0.5%, and treat them at 98-100°C for 40 minutes to remove the external sericin, and obtain pure silk fibroin after fully washing. The dried silk fibroin is dissolved in a 9.3 mol / liter lithium bromide solution at about 60° C. to obtain a silk fibroin mixed solution.

[0046] The silk fibroin mixed solution is added to the cellulose dialysis membrane, and deionized water is used for dialysis to remove lithium bromide to obtain a pure silk fibroin aqueous solution.

[0047] Place the silk fibroin solution at 60°C for 24 hours, then adjust the concentration of silk fibroin to 2%, and place it at 4°C for 1 hour to stabilize the temperature of the solution at 4°C.

[0048] Dilute the collagen solution to 0.2%, and place it at 4°C for 1 hour to stabilize the temperature of the solution at 4°C.

[0049] Under the condition of 4°C, the same volume of silk fibroin solution an...

Embodiment 3

[0054] Add 5 grams of silkworm cocoons into 2 liters of sodium carbonate solution with a mass concentration of 0.5%, and treat them at 98-100°C for 40 minutes to remove the external sericin, and obtain pure silk fibroin after fully washing. The dried silk fibroin is dissolved in a 9.3 mol / liter lithium bromide solution at about 60° C. to obtain a silk fibroin mixed solution.

[0055] The silk fibroin mixed solution is added to the cellulose dialysis membrane, and deionized water is used for dialysis to remove lithium bromide to obtain a pure silk fibroin aqueous solution.

[0056] Place the silk fibroin solution at 60°C for 24 hours, then adjust the concentration of silk fibroin to 1%, and place it at 4°C for 1 hour to stabilize the temperature of the solution at 4°C.

[0057] Dilute the collagen solution to 0.075%, and place it at 4°C for 1 hour to stabilize the solution temperature at 4°C.

[0058] Under the condition of 4°C, the same volume of silk fibroin solution and col...

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Abstract

The invention relates to a preparation method of a porous scaffold material and discloses a preparation method of a nano-fibrous silk fibroin-based porous scaffold. The preparation method comprises the following steps: blending a silk fibroin solution and a collagen solution under the temperature of 0-20 DEG C, standing to ensure the complete reaction of collagen and silk fibroin and generate nano-fiber; freezing the mixed solution to obtain a freezing body; performing freeze drying treatment on the freezing body to obtain a nano-fibrous porous scaffold material; and placing the nano-fibrous porous scaffold material in a vacuum drier with water and methanol, or an ethanol aqueous solution at the bottom, vacuumizing, and then standing to obtain the water-insoluble nano-fibrous porous scaffold. The obtained porous scaffold has 10-1000 microns of communicated pores. The pore walls consist of 10-100nm of nano-fibers; and the porous scaffold has similar nanoscale structures and micronscalestructures to extracellular matrix, is suitable for the adhesion and growth of cells and has good biocompatibility.

Description

technical field [0001] The present invention relates to a method for preparing a porous scaffold material, in particular to a technique for preparing a nanofibrous porous scaffold with a structure similar to that of an extracellular matrix using silk fibroin as a raw material. The prepared material can be used in biomedicine, Tissue repair, drug release, tissue engineering and other technical fields. Background technique [0002] Biomedical materials are the research direction and application field with the most development potential in the next ten years. Biomaterial porous scaffolds for tissue engineering and tissue repair should not only have good biocompatibility and degradability, but also have a suitable multi-level structure, so as to regulate cell behavior and promote normal tissue regeneration. The extracellular matrix is ​​the best template for the preparation of porous scaffolds, which are mainly composed of nanofibers, and further form a porous structure with po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/24A61L27/22
Inventor 吕强罗峥
Owner SUZHOU UNIV
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