428 results about "ECM Protein" patented technology

The present invention relates to a preparation method of a collagen filler for injection. Non-crosslinked collagen, crosslinked collagen, and biodegradable polymer microspheres are compounded and mixed in a certain proportion to achieve an immediate filling effect. The biodegradable polymer microspheres play a long-term role, and the amino acid components degraded from collagen in the early and middle stages can become nutrients that stimulate the production of collagen regeneration by polyester microspheres. In the present invention, the crosslinked collagen has a high yield and less water loss through a gradient irradiation crosslinking technique. The non-crosslinked collagen gel can provide a microenvironment for nutritional repair and a filling effect in the early stage, the crosslinked extracellular matrix can provide a repair microenvironment in the middle stage of filling, and the degradation of polyester microspheres in the later stage promotes collagen regeneration, enabling the skin to have a good microenvironment throughout the repair cycle and increasing skin elasticity.

The invention belongs to the technical field of biomedical tissue engineering materials, and particularly relates to injectable composite plastic gel and a preparation method thereof. The injectable plastic gel provided by the invention is formed by suspending and compounding the cross-linked sodium hyaluronate gel particles and the biological tissue extracellular matrix particles, the two material particles in the injectable composite plastic gel are uniform in size and controllable in size, other toxic catalysts do not need to be added in the preparation process, and the injectable plastic gel is easy to prepare. The collagen / elastin composite material is rich in collagen and elastin, and shows good biomechanical properties. Meanwhile, the composite plastic gel contains a plurality of bioactive factors, and after the composite plastic gel is injected and filled into a body, the composite plastic gel is slowly degraded and can promote the generation and ingrowth of autologous fat cells and microvessels, so that the balance of degradation and remodeling is achieved. The preparation method of the injectable plastic gel provided by the invention can be widely applied to the fields of plastic surgery, medical beauty and the like.

The invention provides a superfine-diameter PCL / COL nanofiber membrane as well as a preparation method and application thereof, and relates to the technical field of skin wound repair materials. The PCL / COL nanofiber membrane material is prepared from PCL and I-type collagen as raw materials through electrostatic spinning and vacuum drying. According to the preparation method disclosed by the invention, an electrostatic spinning technology is combined with strict regulation and control on parameters, so that the stable and superfine 40-nanoscale PCL / COL nanofiber membrane material is prepared. The diameter of the superfine PCL / COL nanofiber membrane material reaches the bionic scale of the minimum natural collagen of an extracellular matrix, and meanwhile, the superfine PCL / COL nanofiber membrane material shows more excellent hydrophilicity and lower surface roughness, has good biological safety and mechanical stability, not only realizes high matching with a natural ECM hierarchical structure, but also has good application prospects. By enhancing the cell-material interface interaction, the adhesion, proliferation and migration capacities of fibroblasts are remarkably improved, the wound healing period can be effectively shortened, an excellent repair promoting effect is shown, and a solid foundation is laid for clinical transformation of materials.

The invention provides an in-vitro skin blood vessel immune model as well as a preparation method and application thereof. THP-1 human monocyte leukemia cells are induced to be differentiated into M0 type macrophages, and then the M0 type macrophages are respectively induced into M1 type macrophages and / or M2 type macrophages; then co-culturing the M1 type and / or M2 type macrophages and vascular endothelial cells to form a vascular immune model; finally, the 3D skin model is placed on the blood vessel immune model, external stimulation is conducted, and the in-vitro skin blood vessel immune model is constructed. The in-vitro skin blood vessel immune model can be used for repairing skin barriers, inflammation pathways, blood vessel metabolism, the expression level of genes or proteins related to extracellular matrixes, vascular endothelial cells, the proliferation and migration ability of activated macrophages and the like. The seven feature dimensions of the physiological structure feature of the skin model are used for screening the to-be-tested sample and exploring the action mechanism, and the method has the characteristics of rapidness, multiple screening dimensions, high accuracy, low construction difficulty, low cost and high universality.

The present invention provides: a drug which can improve the implantation rate of an embryo and can be used in conjunction with conventional drugs for infertility treatment; and a screening method and the like with which it is possible to reliably evaluate such a drug. Specifically, the integrin activator comprises: (1) a peptide composed of KFEERMRCKWMT, and (2) a peptide composed of KFEERMRCKWMT. (2) a peptide which is composed of KFEEERSRCKWMT; (3) a peptide comprising an amino acid sequence obtained by deleting, substituting and / or adding 1-3 amino acids in (1) or (2); or (4) a peptide obtained by binding any one of (1) to (3) to a membrane-permeable molecule. Provided are an artificial uterus and a screening method based on an implantation reproduction test system using a fertilized egg (for example, an embryo developed to an implanted blastocyst), the artificial uterus comprising: a hydrogel containing endometrial stromal cells and having an extracellular matrix as a main component; and a uterine epithelial organoid configured to be surrounded by the hydrogel.

The invention provides a polypeptide compound and application thereof, and belongs to the technical field of medical or dressing preparations. According to the invention, three specific polypeptides are synergistically proportioned to obtain the polypeptide compound, and the polypeptide compound has the effect of repairing a skin basement membrane in a targeted manner. The polypeptide compound has the advantages of being small in molecular weight, high in safety, low in allergy risk and high in permeability, and has excellent repairing and anti-aging functions. Experiments prove that the polypeptide compound provided by the invention, as an active peptide raw material, can remarkably promote the production of endogenous collagen IV, VII and XVII of skin, is also beneficial to repairing skin extracellular matrix, resisting DNA damage, improving skin cycle protein, inhibiting release of inflammatory factors and the like, can effectively repair a basement membrane, and has a wide application prospect.

The invention belongs to the technical field of biological medicine, and particularly relates to recombinant human XVII micromolecular collagen as well as a preparation method and application thereof. According to the invention, a recombinant human XVII type micromolecular collagen (Re-Hcol17) is constructed, and a stable triple helix structure is formed by connecting the 15th collagen domain, the 12th collagen domain, the 5th collagen domain and the 1st collagen domain in series. Experimental results prove that the Re-Hcol17 can significantly promote NIH / 3T3 fibroblast proliferation within the concentration range of 10-100 [mu] g / mL, the effect is better than that of natural human collagen, and extracellular matrix adhesion can be effectively promoted. Through genetic engineering and fermentation process innovation, the bottleneck that the human-derived XVII type collagen is difficult to efficiently express and unstable in structure is broken through, and a foundation is laid for wide application of the human-derived XVII type collagen in the fields of biological medicines and cosmetics.

The invention relates to the technical field of bone tissue materials, and discloses a metal-doped biomimetic mineralized dECM cryogel and a preparation method and application thereof.The preparation method of the cryogel comprises the steps that firstly, hyaluronic acid and methacrylic anhydride are mixed in a solvent, the pH value is adjusted, a reaction is conducted, and methacrylic acid hyaluronic acid is obtained; then under the condition of a photoinitiator, mixing the methacrylic acid hyaluronic acid with an acellular extracellular matrix, and performing freezing and crosslinking treatment to obtain the cryogel, and finally, soaking the cryogel in a mineralization solution containing Ca < 2 + > and / or Mg < 2 + >, adding a buffer solution containing phosphite ions, incubating, and washing to obtain the crystal gel. The biomimetic mineralized dECM cryogel prepared by the method disclosed by the invention is good in ion release stability, can significantly improve the migration ability of HUVECs, contributes to promoting proliferation of rMSCs and improving the osteogenic differentiation potential of the rMSCs, and can be used for regeneration and repair of bone defects.

A collagen membrane with an effect of inducing ordered remodeling of tissues, a preparation method therefor, and use thereof, relating to the technical field of surgical supply materials. The preparation method for the collagen membrane comprises the following steps: subjecting a fiber membrane to a heat pressing treatment to obtain the collagen membrane with the effect of inducing ordered remodeling of tissues. The fiber membrane is formed by cross-stacked fibers with a diameter of 0.5-15 μm. The fibers comprise collagen and poly(L-lactide-co-caprolactone). The mass ratio of the collagen to the poly(L-lactide-co-caprolactone) is 1:(1.5-2.5). By combining the collagen, which has good biocompatibility and an effect of promoting tissue repair, with PLCL, which is biodegradable and features excellent mechanical properties and good cell adhesion, the collagen membrane not only has an extracellular matrix-like structure, but also exhibits good structural stability, facilitating the regulation of cell behaviors and tissue regeneration, and effectively promoting the growth, adhesion, and proliferation of host cells, thereby inducing ordered remodeling of tissues.

The invention belongs to the technical field of biomedical materials and regenerative medicine, and relates to collagen-based injectable fiber hydrogel as well as a preparation method and application thereof. According to the injectable fiber hydrogel, methacrylated collagen and dithiothreitol are subjected to a thiol-ene click reaction rapidly under the action of a photoinitiator through illumination, a primary cross-linked network is formed, and the injection and in-situ gel forming requirements are met; then under the conditions that the pH is 6.5-8.0 and the temperature is 30-39 DEG C, collagen molecules are self-assembled into a nanofiber structure, and a bionic network similar to a natural extracellular matrix is obtained. The hydrogel disclosed by the invention has the advantages of rapid gelation, structural biomimetic property and biological activity; residual sulfydryl endows the hydrogel with active oxygen scavenging capacity, so that oxidative stress can be relieved, and immune response can be regulated; the fibrotic structure promotes cell adhesion, migration and tissue regeneration.

The invention belongs to the technical field of biology, and discloses a fusion type polypeptide for improving the activity of umbilical cord mesenchymal stem cells and application of the fusion type polypeptide. The amino acid sequence of the fusion type polypeptide provided by the invention is any one sequence as shown in SEQ ID NO.1-3. The fusion type polypeptide is obtained by integrating an extracellular matrix amino acid sequence and a heparin amino acid sequence. The umbilical cord mesenchymal stem cells obtained by culturing the fusion type polypeptide have good multiplication capacity, can promote expression of the Ki67 gene and inhibit aging of the umbilical cord mesenchymal stem cells, and have wide application prospects.

The invention discloses a culture system, culture method and application of gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system comprises: a basic culture medium, which is a DMEM / F12 culture medium, and is additionally added with a special growth promoting factor obtained from a natural extract; the multiple growth factors comprise an epidermal growth factor (EGF), a fibroblast growth factor (FGF), a bone morphogenetic protein (BMP) and a vascular endothelial growth factor receptor inhibitor (VEGFRi), and the concentration of each growth factor is accurately screened and optimized; the extracellular matrix is Matrigel modified by physical and chemical methods; the specific additive comprises an antioxidant N-acetylcysteine (NAC). According to the culture system, the culture method and the application of the gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system which is closer to a real environment in vivo is constructed by optimizing basic culture medium components, setting various growth factors and specific additive concentrations and adopting modified Matrigel as an extracellular matrix.

The invention belongs to the technical field of biological medicines, and particularly relates to a recombinant polypeptide for improving the activity of bone marrow mesenchymal stem cells and application of the recombinant polypeptide. The amino acid sequence of the recombinant polypeptide is as shown in SEQ ID NO.3. The recombinant polypeptide is obtained by integrating an extracellular matrix amino acid sequence and a heparin amino acid sequence. The recombinant polypeptide provided by the invention can promote the expression of the Ki67 gene and improve the proliferative activity of the bone marrow mesenchymal stem cells, also has the effect of inhibiting the replicative aging of the bone marrow mesenchymal stem cells, and has a wide application prospect.

The invention discloses a novel absorbable wound dressing and a preparation method thereof. The novel absorbable wound dressing is prepared from collagen, acellular extracellular matrix powder and purified water. The novel absorbable wound dressing with activity is prepared by compounding the acellular extracellular matrix powder and the collagen, the acellular extracellular matrix powder contains various growth factors, various growth factors do not need to be additionally added, and compared with a common collagen sponge dressing, the novel absorbable wound dressing has the better effect of promoting wound healing, and the wound healing effect is better. Meanwhile, collagen is directly adopted as a hemostasis carrier of the acellular extracellular matrix powder and is subjected to freeze-drying forming, seepage of the wound surface can be absorbed, the mechanical strength of the acellular extracellular matrix powder can be enhanced, and therefore the wound can be rapidly and effectively bonded, and the hemostasis effect is achieved. The safety is high; and the manufacturing method is simple.

The invention discloses a composite gel for skin correction and a preparation method thereof. The composite gel is formed by dissolving sodium hyaluronate, epidermal growth factors and a protein stabilizer in a buffer system; the content of the sodium hyaluronate is 1.0-50.0 g / L, the content of the epidermal growth factor is 1000-30000 IU / mL, and the content of the stabilizer is 5.0-100.0 mg / L; the pH value of the buffer system is 6.5-7.5. The preparation method comprises the following steps: S1, dissolving sodium hyaluronate and a stabilizer in a buffer system under a sterile condition, and stirring to obtain gel A; s2, adding epidermal growth factors into the gel A, and stirring to obtain the composite gel. The composite gel disclosed by the invention can activate epithelial fibroblasts, update and reconstruct ECM and maintain the stability of an ECM microenvironment. The composite gel disclosed by the invention integrates the functions of supplementing lost extracellular matrix, increasing the number of cells and promoting synthesis of collagen by a traditional filling material, can solve the skin problems caused by various factors, and achieves the effect of correcting the skin.

The invention discloses a multi-functional domain fusion extracellular matrix protein as well as a coding gene, a preparation method and application thereof, and belongs to the fields of biotechnology, medical cosmetology and wound repair. The multi-functional-domain fusion extracellular matrix protein provided by the invention contains core functional domains of human-derived III-type collagen, elastin and fibronectin, integrates key biological functions of the human III-type collagen, elastin and fibronectin, and is obtained by expression of mammalian cells. The method can promote formation of a collagen triple helix structure, is simple in process, high in yield and easy for industrial production, and can be widely applied to the fields of effective skin care, skin aging resistance, medical cosmetology, wound repair and the like.

The invention discloses a dual-network injectable hydrogel as well as a preparation method and application thereof. The hydrogel is methacrylated gelatin / oxidized hyaluronic acid loaded with metal polyphenol nano-particles, and the metal polyphenol nano-particles are copper-gallic acid nano-particles which contain quaternized chitosan coatings and are loaded with antibacterial peptide HHC36. After the hydrogel is injected into a periodontal pocket, the hydrogel can adapt to different defect shapes, and the CuGA-coated HHC36-coated QCS nanoparticles are released by responding to a periodontitis microenvironment, so that the effects of resisting periodontal pathogenic bacteria, removing local excessive ROS (reactive oxygen species), effectively promoting fibroblast proliferation, collagen deposition, extracellular matrix accumulation and angiogenesis and further promoting periodontal bone regeneration are achieved. The preparation process is simple, the injectable hydrogel is low in price and easy to obtain, the injectable hydrogel can be prepared through simple steps, and a new thought is provided for treatment of periodontitis.

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The invention relates to the technical field of medical nano-materials, and discloses an iron-manganese bimetallic MOFs nano-enzyme hydrogel compound and a preparation method and application thereof, and the method comprises the following steps: step 1, preparing FeMn bimetallic MOFs nano-enzyme MIL-101 by adopting a microwave-assisted solvothermal method; step 2, preparing oxidized hyaluronic acid; step 3, synthesizing a hydrogel precursor protocatechuic aldehyde and a complex PA-Fe of iron ions; and step 4, fully mixing the quaternary ammonium salt chitosan solution with the PA-Fe solution, adding the oxidized hyaluronic acid solution and the MIL-101 powder under continuous magnetic stirring, and finally forming the iron-manganese bimetal MOFs nano-enzyme hydrogel compound. The delivery efficiency of the bimetallic nano-enzyme is improved, the in-vivo retention time of the bimetallic nano-enzyme is prolonged, the bimetallic nano-enzyme has antibacterial, hemostatic, adhesion and anti-oxidation effects, the steady state of an extracellular microenvironment of the intervertebral disc is recovered, an extracellular matrix of intervertebral disc tissue is remodeled, and the effect of promoting the repair and regeneration of the intervertebral disc by the nano-enzyme is synergistically enhanced.

The present invention provides A method of producing a decellularised extracellular matrix (ECM) scaffold of at least a portion of a lobular organ with no common artery, the method comprising: a) closing afferent blood vessels to substantially seal a target lobular organ or portion thereof with no common and / or major artery within a non-human donor or a dead / brain dead human donor; b) optionally: (i) cleaning coagulum and / or blood from at least a portion of the closed afferent blood vessels; and / or (ii) perfusing the organ or portion thereof to confirm closure of the afferent blood vessels; c) removing the sealed organ or portion thereof from the donor; and d) perfusing the sealed organ or portion thereof with detergent and enzymatic solutions to obtain the decellularised ECM scaffold. Methods for producing an artificial organ, and artificial organs produced by the methods are also provided.

The invention provides an in-situ tissue engineering valve stent material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the invention, the surface of the acellular matrix is loaded with the super-particle coating formed by self-assembly of the polyphenol-mediated polypeptide aggregate and the Prussian blue nano-enzyme, so that the stent material is endowed with double ROS regulation and endothelial cell adhesion promoting functions, excessive deposition of collagenous fibers can be prevented, and fibrosis of valve tissues is avoided; the synergistic effect of the components of the super-particle coating promotes the endothelialization process of the stent and the remodeling of a functional extracellular matrix, so that the accelerated formation of the functional endodermis is finally realized, and a powerful guarantee is provided for the success of clinical transformation of an in-situ heart valve tissue engineering technology.

The invention discloses a carbon dot, hydrogel containing the carbon dot as well as a preparation method and application of the hydrogel, and relates to the technical field of carbon materials. The carbon dot is prepared from the following raw materials: a hydroxyl-containing flavonoid compound and epsilon-polylysine; the carbon dots contain aniline bonds formed by hydroxyl groups of the hydroxyl-containing flavonoid compounds and amino groups of the epsilon-polylysine. The carbon dot shows higher tumor cell killing property and lower normal cell toxicity than the raw material hydroxyl-containing flavonoid compound at the cellular level, can obviously induce immunogenic death of cancer cells and does not induce up-regulation of extracellular matrix collagen, and also has a better effect of regulating a tumor immune microenvironment.

The invention relates to the technical field of gel, in particular to exosome-loaded temperature-sensitive hydroxybutyl chitosan hydrogel as well as a preparation method and application thereof.The HBC-SH hydrogel is formed by self-assembly of hydroxybutyl chitosan and silk fibroin, and under the action of two components, the hydrogel is higher in gelation speed and better in mechanical property, so that the temperature-sensitive hydroxybutyl chitosan hydrogel can be used for preparing the hydrogel. According to the invention, Exo is wrapped in microspheres by adopting a microencapsulation technology, and then the microspheres are stably encapsulated in HBC-SH hydrogel, so that the residence time and the stability of the exosome are prolonged, the longer-time slow release is realized, and the wound repair effect is improved. HBC-SH hydrogel can simulate the environment of a natural extracellular matrix and promote cell adhesion, migration and proliferation, so that tissue repair is accelerated, genetic information can be transmitted through Exo, tissue regeneration is further promoted, growth factors and cell factors of Exo and bioactive components in hydroxybutyl chitosan and silk fibroin generate a synergistic effect, and the effect of promoting tissue regeneration is achieved. And wound repair, cartilage repair and nerve regeneration processes are promoted together.

An implantable medical product and method of use for substantially reducing or eliminating harsh biological responses associated with conventionally implanted medical devices, including inflammation, infection and thrombogenesis, when implanted in in a body of a warm blooded mammal. The bioremodelable pouch structure is configured and sized to receive, encase and retain an electrical medical device therein and to allow such device to be inserted into the internal region or cavity of the pouch structure; with the pouch structure formed from either: (a) first and second sheets, or (b) a single sheet having first and second sheet portions. After receiving the electrical device, the edges around the opening are closed by suturing or stapling. The medical device encased by the bioremodelable pouch structure effectively improves biological functions by promoting tissue regeneration, modulated healing of adjacent tissue or growth of new tissue when implanted in the body of the mammal.

The invention relates to the technical field of biology, in particular to a composition, a culture medium and application of the culture medium in intestinal organ culture. The glucosamine hydrochloride is applied to culture of intestinal organs for the first time, organ proliferation and differentiation can be effectively promoted, and the number of formed organs is remarkably increased. On the basis, a three-dimensional culture system for staged time sequence induction in a postpartum newborn period, a child period and an adult period is constructed, and accurate construction of intestinal organs of the mice in different development periods is realized by combining dynamic regulation and control of an extracellular matrix and combinatorial optimization of growth factors; and an in-vitro model covering multiple time sequence stages is provided for intestinal development mechanism research and disease modeling.

The invention discloses an anti-aging transdermal drug delivery microneedle preparation solution, a preparation method and application of the anti-aging transdermal drug delivery microneedle preparation solution and a transdermal drug delivery soluble microneedle, and belongs to the technical field of microneedle preparation. Comprising 0.25%-10% of an anti-sugar and anti-oxidation component, 0.1%-10% of an anti-ultraviolet damage component, 0.1%-10% of a cell and collagen regeneration component, 10%-30% of an extracellular matrix supplement component, 0.05%-3.1% of a nerve inhibition component and the balance of water. The transdermal drug delivery microneedle preparation solution disclosed by the invention can be used for preparing a transdermal drug delivery soluble microneedle, and the transdermal drug delivery soluble microneedle can be used for rapidly improving the intercellular capacity, so that the effects of improving the material transportation speed, the cell division and differentiation speed and the construction speed of extracellular matrixes such as collagen and the like are achieved; therefore, the purpose of recovering the tough and elastic young state of the skin is achieved.

The present disclosure provides a polypeptide complex comprising a target binding polypeptide that binds to a molecule on a target cell or a molecule in an extracellular matrix (ECM); and small latent complexes comprising in particular a dimer latent related polypeptide (LAP) or a fragment or derivative thereof, and a dimer mature transforming growth factor beta (TGF beta) family polypeptide or a fragment or derivative thereof. Polypeptides (e.g., fusion polypeptides), and related polynucleotides, vectors, cells, and pharmaceutical compositions are also provided. Also provided are methods of treating subjects using, for example, these polypeptide complexes and / or these fusion polypeptides or pharmaceutical compositions thereof.

A three-dimensional mammary gland model comprising a three-dimensional tissue comprising cells and fragmented extracellular matrix components, the cells comprising mature adipocytes and mammary gland epithelial cells.

The invention discloses an implantable acellular matrix material and a preparation method thereof, and belongs to the field of biomedical materials. According to the method, natural animal tissues (such as pericardium, achilles tendon or small intestine submucosa) are used as raw materials, a low-temperature chemical combined decellularization treatment technology is adopted, and the method comprises the multi-step synergistic process of pre-cooling soaking balancing, low-temperature enzymolysis, low-concentration surfactant gradient permeation cleaning, low-temperature nuclease degradation, non-crosslinking sterilization and the like. Under the condition that the treatment temperature is strictly kept at 0-8 DEG C, cell components and genetic materials (the residual DNA content is 1t, and the dry weight is 8 ng / mg) are efficiently removed, and meanwhile the natural three-dimensional fine structure of an extracellular matrix (ECM), the integrity of collagen fibrils and key bioactive components (such as glycosaminoglycans (GAGs)) are reserved to the maximum extent. The host immunological rejection reaction of the obtained material is remarkably reduced, host cell ingrowth, vascularization and tissue function reconstruction can be effectively promoted after implantation, and the material is suitable for high-end implantation scenes such as soft tissue repair and regenerative medical stents.

The present invention provides constructs including a tubular biodegradable polyglycolic acid scaffold, wherein the scaffold may be coated with extracellular matrix proteins and substantially acellular. The constructs can be utilized as an arteriovenous graft, a coronary graft, a peripheral artery bypass conduit, or a urinary conduit. The present invention also provides methods of producing such constructs.