79 results about "Matrix metalloproteinase" patented technology

The invention discloses an abdominal aortic aneurysm progress prediction method and system based on cross-modal knowledge distillation. The method comprises the following steps: collecting blood of a target object; the method comprises the following steps: detecting the concentration of blood protein fingerprint markers of blood of a target object, wherein the blood protein fingerprint markers comprise matrix metalloproteinase-12, calcitonin-related polypeptide-alpha, uromodulin, nerve injury induction protein-1, glycosylated hemoglobin A1c and fibroblast growth factor-9; carrying out abdominal aortic aneurysm risk calculation by calling Student-net of cross-modal knowledge distillation based on the blood protein fingerprint marker concentration; wherein the input of the Teaser-net of the cross-modal knowledge distillation comprises a CTA image feature and a pathological section feature, and a category probability vector output by the Student-net is aligned with a category probability vector output by the Teaser-net; and outputting the risk level of the abdominal aortic aneurysm and the probability of occurrence / progress / rupture of the abdominal aortic aneurysm in the preset year. And the development of the aortic aneurysm disease is accurately predicted.

The invention discloses a tightening and whitening composition containing EGCG (epigallocatechin gallate) as well as a preparation method and application of the tightening and whitening composition, and relates to the technical field of cosmetics. The invention provides a tightening and whitening composition containing EGCG (epigallocatechin gallate). The tightening and whitening composition is prepared from the following components in parts by weight: 0.1 to 2 parts of an epigallocatechin gallate nano composition, 0.05 to 0.5 part of a nucleic acid tetrahedron and 0.5 to 5 parts of glassine. When the EGCG nano composition, the nucleic acid tetrahedron and the glassine are cooperatively used, the stability of a formula can be improved, meanwhile, the EGCG can be accurately released to an epidermal layer due to the enzyme-responsive degradation characteristic (such as matrix metalloproteinase MMP-2 triggered depolymerization) of the nucleic acid tetrahedron, and the glassine is released after deeply penetrating into a corium layer along with a nucleic acid tetrahedron carrier, so that the EGCG can be accurately released to the epidermal layer. The synergistic effects of the three components on whitening and tightening are better exerted.

The invention discloses an oligonucleotide intracellular nano delivery system based on polypeptide modification and application thereof. The system is composed of a periostin targeting sequence (SDSSD), a matrix metalloproteinase 2 (MMP2) response sequence (GPAGLLG), a cell penetrating sequence (RRRRRRRR, R9), a reactive oxygen species (ROS) scavenging and adhesion enhancing group (Gly-DOPA)) and a terminal dibenzocyclooctyne (DBCO) modified engineered polypeptide SDSSD-PEG5-YGFGG-GPAGLLG-R9-(G-DOPA) 3-K4-C-DBCO, and a target oligonucleotide miRNA-26a-A5-Azido modified by 5-polyadenylic acid (AAAAA) and an azide group (Azido), and the target oligonucleotide miRNA-26a-A5-Azido, the target oligonucleotide and assembling through a click chemical reaction and a non-covalent interaction. The nano system has good bone targeting, enzyme responsiveness, intracellular delivery effect and biological safety, can realize stable and efficient delivery of therapeutic oligonucleotides in vivo, and significantly improves the utilization efficiency and therapeutic potential of oligonucleotides. The oligonucleotide intracellular nano delivery system has a wide application prospect in the fields of clinical transformation and precise treatment of oligonucleotide drugs.

The invention belongs to the field of biological medicines, and finds and verifies that taquinimod or pharmaceutically acceptable salts thereof can be used for preparing medicines for treating endometriosis for the first time, and is particularly suitable for progestogen-resistant, recurrent or deep infiltration type endometriosis. The action mechanism of the taquinimod is as follows: the taquinimod can down-regulate the expression of S100A9 protein and reduce the infiltration of S100A9 + macrophages by inhibiting an NF-kappa B signal channel so as to further inhibit the expression of a vascular endothelial growth factor receptor 1 (VEGFR1) and a matrix metalloproteinase 2 (MMP2), finally block pathological angiogenesis and inhibit the growth of ectopic lesions. Animal experiments show that the taquinimod can significantly reduce the number and weight of nidus of a mouse model with endometriosis. The invention provides a brand-new non-hormone treatment choice with a clear action mechanism for clinical application, provides a pharmaceutical composition containing a specific dosage and a drug combination scheme, and has an important clinical value.

The invention relates to the technical field of biological active peptides, in particular to a method for rapidly and effectively screening light protection active peptides from collagen hydrolysate. The invention relates to an application of a photoprotective polypeptide in preparation of a product. The product has at least one of the following purposes: (1) maintaining or increasing the water content of skin; (2) preventing and / or slowing down skin aging; (3) prevention and / or alleviation of skin UVB injury; and (4) promoting healing of skin wounds or wound surfaces. Two key signal factors (NF-kB, AP-1) and a key matrix metalloproteinase (MMP-9) are screened from a mitogen-activated protein kinase (MAPK) and nuclear factor-kB (NF-kB) pathway to be used as docking targets, and a mimic peptide and the three targets act synergistically to make up for the defect that a single target screening peptide is difficult to comprehensively cope with a light damage multivariate pathway, so that the application of the mimic peptide to the light damage multivariate pathway is realized, and the application of the mimic peptide to the light damage multivariate pathway is realized. And the collagen peptide with excellent light protection activity is obtained through screening.

The invention discloses iron oxide nanoparticles capable of responding to particle size change in a tumor microenvironment and a preparation method of the iron oxide nanoparticles, and belongs to the technical field of biomedical nano materials. The nano-particle comprises an iron oxide nano-crystal core with the particle size of 3-5nm and a tumor microenvironment response layer coating the surface of the iron oxide nano-crystal core. The response layer contains groups, such as disulfide bonds, enzyme digestion peptide fragments and the like, which can respond to acidic pH, high-concentration reduced glutathione or matrix metalloproteinase and other tumor characteristic stimuli to generate structural changes. In a tumor microenvironment, the hydration particle size of the nanoparticles can be directionally and controllably converted from 3-20 nm to 20-100 nm (or reversely). The preparation method mainly comprises two steps of preparing the core by a thermal decomposition method and modifying the surface response layer. The technical problems of insufficient tumor targeted enrichment capacity, limited imaging contrast and difficulty in balancing permeation and retention caused by fixed particle size of the existing iron oxide nanoparticles are solved, and the accuracy and efficiency of tumor diagnosis and treatment can be remarkably improved.

The invention relates to the field of nutritional health food, and discloses a casein modified active peptide for promoting bone repair and a preparation method thereof.The structure of the active peptide sequentially comprises a bone targeting sequence with high affinity to hydroxyapatite, a casein phosphopeptide core sequence capable of chelating calcium ions, a casein phosphopeptide core sequence capable of chelating calcium ions, a casein phosphopeptide core sequence capable of chelating calcium ions, a casein phosphopeptide core sequence capable of chelating calcium ions and a casein phosphopeptide core sequence capable of chelating calcium ions from the C-terminal to the N-terminal. The digestion sequence can be specifically hydrolyzed by matrix metalloproteinase, and the alkynyl functional group is used for covalent cross-linking. The active peptide can be mixed with an azido-containing multi-arm cross-linking agent, and hydrogel can be rapidly formed under physiological conditions through cycloaddition reaction. The system can be gelated in situ and anchored to a bone defect part in a targeted manner, and is triggered by a specific enzyme in a pathological microenvironment to controllably release active peptide fragments capable of chelating calcium ions, so that the concentration of local mineral ions is adjusted, the cell living environment is improved, and an effective new way is provided for promoting bone repair.

The invention discloses a functional lipid compound for nano-liposome wrapping and a preparation method, and relates to the technical field of functional lipid compounds, the functional lipid compound comprises (a) a polyphenol-lipid covalent conjugate which is composed of a polyphenol active unit selected from curcumin, dihydroquercetin or derivatives thereof, and (b) a lipid covalent conjugate which is composed of a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof and a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof; the conjugate is covalently linked to a phosphatidyl ethanolamine or cholesterol skeleton through a cleavable linking group, and the conjugate can be integrated into a nano-liposome membrane and has the functions of structural support and biological activity; (b) a microenvironment-responsive functional phospholipid, which is a phospholipid derivative having a functional group responsive to an inflammatory microenvironment specific stimulus modified on a hydrophilic head group or a hydrophobic chain of a phospholipid molecule, the specific stimulus being selected from local pH < = 6.5, reactive oxygen species (ROS) concentration > = 10 [mu] M or matrix metalloproteinase MMP-2 / 9 overexpression; and (c) a bionic exosome membrane lipid component, wherein the bionic exosome membrane lipid component comprises sphingosine-based glycolipid or sulfated cholesterol separated from the plant-derived exosome.

The invention provides an antibody targeting synergistic MMP intelligent response drug delivery system as well as a preparation method and application thereof. Based on the characteristics of a tumor microenvironment, a B7H3 targeting / MMP responsive drug delivery system is constructed, a B7H3 targeting antibody is modified to actively target a tumor cell surface antigen B7H3 to be combined with the B7H3 targeting antibody, the enrichment degree of a nano-drug in a tumor focus is improved through precise targeting, and the drug delivery efficiency is improved. Further, under the action of matrix metalloproteinase with high specificity expression in a tumor microenvironment, the designed and screened intelligent responsive polypeptide is subjected to enzymolysis to break so as to expose cationic groups, so that charge overturning of the nano-drug is promoted, and the stable nano-drug with negative charges in a physiological blood circulation system is converted into a nano-drug with positive charges; the medicine is endowed with the capacity of deeply penetrating into solid tumors, the treatment effect of the medicine on tumors is improved by breaking through a matrix barrier, and a new method is provided for treating solid tumors represented by pancreatic cancer.

The invention discloses an application of eclipta source extracellular vesicles. The extracellular vesicles are extracted from the eclipta by adopting an overspeed combined ultrafiltration centrifugation method, and the extracted vesicles are complete in morphology and good in dispersity, and have the characteristics of high yield, high separation speed, low cost and easiness in large-scale production. The extracted vesicles can be successfully taken by animal cells. The yerbadetajo herb alcohol extract has the advantages that the yerbadetajo herb alcohol extract has antioxidant and anti-aging effects after being taken in, ultraviolet-induced matrix metalloproteinase 1 (MMP1) expression can be obviously reduced, collagen I (COL-I) generation can be promoted, ultraviolet-induced skin light aging can be improved, and the effect is better than that of the yerbadetajo herb alcohol extract, so that the yerbadetajo herb alcohol extract can be used for preparing the skin The eclipta-derived extracellular vesicles provided by the invention provide a new strategy for skin aging and anti-aging treatment.

The present invention relates to a novel Lactobacillus acidophilus strain. Also, the present invention relates to uses of the Lactobacillus acidophilus strain for antibacterial activity, antioxidation, the suppression of periodontal tissue damage or degradation, and anti-inflammation. Also, the present invention relates to a composition comprising the Lactobacillus acidophilus strain. The Lactobacillus acidophilus strain of the present invention can exhibit antibacterial activity against oral harmful bacteria that cause oral diseases, suppress the production of reactive oxygen species (ROS), inhibit the expression or activity of matrix metalloproteinase-3 (MMP-3) that causes periodontal tissue damage or degradation, and inhibit the expression or activity of inflammatory cytokines IL-6 and IL-8. Therefore, the strain and the composition comprising same, of the present invention, can be effectively used for antibacterial activity, antioxidation, the suppression of periodontal tissue damage or degradation, and anti-inflammation, and can also be used for treating, ameliorating or preventing oral diseases.

The invention discloses an application of senkyunolide I in preparation of a medicine for treating hepatic fibrosis, which can obviously inhibit HSC (hepatic stellate cell) activation in vitro and evaluate the treatment effect of senkyunolide I on hepatic fibrosis in vivo by adopting a C57BL / 6 mouse hepatic fibrosis model induced by CCl4. The expression levels of liver alpha-smooth muscle actin, type I collagen and matrix metalloproteinase tissue inhibition factor 1 of individuals needing anti-hepatic fibrosis are obviously reduced in vivo and in vitro, and the hepatic fibrosis progress can be effectively intervened. Therefore, according to the application of the senkyunolide I in the hepatic fibrosis, the provided compound senkyunolide I has huge potential for inhibiting the hepatic fibrosis, an effective traditional Chinese medicine monomer component medicine is provided for treating the hepatic fibrosis, the new clinical adaptation application of the senkyunolide I is expanded, and the application prospect is good.

The invention relates to the technical field of electrochemical sensing, in particular to a multi-channel electrochemical aptamer sensor and a preparation method and application thereof.The multi-channel electrochemical aptamer sensor adopts a commercial silk-screen printing electrode and is combined with a polymer substrate material for amplifying electrochemical signals, and the electrochemical aptamer sensor is prepared. According to the present invention, the corresponding aptamer is fixed by using the gold nanoparticle through the chemical bond so as to specifically detect the three hemorrhage conversion biomarkers after acute cerebral infarction, such as human matrix metalloproteinase (MMP-9), fibrinogen (FN) and plasminogen activation inhibitor (PAI-1); wherein the FN and the PAI-1 are detected through an electrochemical aptamer sensor method for the first time, and simultaneous detection of the three biomarkers is realized through integration with commercial multi-channel micro electrochemical equipment.

The invention relates to the technical field of skin care products, in particular to a turnip extracting solution and a preparation method and application thereof. The preparation method comprises the following steps: mixing turnip with water, decocting to obtain a decoction, decolorizing, filtering and concentrating to obtain the turnip extracting solution. The turnip extracting solution is used for effectively inhibiting B16 cells from generating melanin and inhibiting the activity of tyrosinase in a melanin synthesis path; the increase of the active oxygen level in HaCaT cells is effectively prevented, and the antioxidant effect is obvious; the inflammation of the SDS surfactant on HaCaT cells is effectively prevented, and the generation of an inflammatory factor TNF-alpha is reduced; the expression of cell natural moisturizing factors, ceramide de novo synthesis rate-limiting enzymes and epidermal barrier related genes is up-regulated; the composition can effectively promote the generation of cell collagen and elastin, reduces the synthesis of matrix metalloproteinase 1 for degrading collagen under ultraviolet radiation, and has an anti-aging effect.

This invention discloses an enzyme- and pH-responsive nucleic acid loading and delivery material and its preparation method. The material can be used for efficient loading and delivery of nucleic acid molecules. The preparation method uses amphiphilic zwitterionic monomers to improve the reverse emulsion polymerization system, enhancing polymerization at the two-phase interface and avoiding the extrusion effect of polymerization in the aqueous core on nucleic acid molecules, thereby improving the loading efficiency of nucleic acid molecules. Further preparation of cross-linking agents MP-CL and CB-CL, which can cleave in response to matrix metalloproteinase II or cathepsin B, endows the nanogel with the ability to respond to charge reversal in the tumor matrix and to release nucleic acid molecules from tumor cells. The acid-sensitive blocks on the amphiphilic monomers give the nanogel lysosomal escape capability. The delivery material can efficiently deliver nucleic acid molecules into cells and exhibits excellent stability and biosafety.

This invention relates to the field of biomedicine and discloses a composition for treating non-small cell lung cancer metastasis that targets replication stress vulnerability and its application. The composition includes a dual-responsive polymer prodrug, which consists of a block copolymer backbone, a matrix metalloproteinase cleavage peptide sequence coupled to the hydrophilic end, and an active molecular group coupled to the hydrophobic end based on an asymmetric grafting structure. The active molecular group comprises a replication stress inducer and an ATR kinase inhibitor. This invention maintains the amorphous phase of the micelle core through the asymmetric grafting structure, eliminating the permeation barrier caused by spontaneous crystallization of components. This amorphous core provides a proton permeation channel, ensuring the synchronous in-situ release of multiple target drugs, thereby blocking the DNA replication fork repair pathway and inducing mitotic catastrophe, maintaining the spatiotemporal overlap of multiple drug interventions in the pathological target area, and avoiding the risk of drug resistance.

This invention discloses a sodium alginate cross-linked matrix metalloproteinase-sensitive peptide-loaded dexamethasone sodium phosphate hydrogel and its preparation method. The dexamethasone-loaded hydrogel has good injectability. After injection, it is cleaved by MMPs at the site of inflammation in an inflammatory environment to release dexamethasone, exhibiting good sustained-release effect. It achieves targeted and long-acting treatment of inflammatory arthritis, osteoarthritis or other inflammatory diseases, and reduces the frequency of repeated intra-articular injections of glucocorticoids.

The present invention discloses engineered platelets carrying platelet activation inhibitor nanoparticles, a preparation method and application thereof, which belongs to the field of pharmaceutical technology and relates to a preparation method of engineered platelets carrying ticagrelor nanoparticles and its therapeutic use in malignant solid tumors. The preparation of the engineered platelets described in the present invention includes the steps of extracting and purifying platelets, preparing gelatin nanoparticles loaded with platelet activation inhibitors, anchoring the nanoparticles to the outer surface of platelets, etc. The engineered platelets of the present invention are targeted to reach the tumor site by recruiting tumor-associated platelets in the body, and specifically respond to matrix metalloproteinases to release the carried platelet activation inhibitor, thereby inhibiting the activation of platelets in the tumor microenvironment, enhancing tumor vascular leakage, alleviating the immunosuppressive microenvironment, and effectively enhancing the effects of chemotherapy and immunotherapy.

The application relates to the field of biological medicine, and discloses ascorbyl palmitate nanoliposomes capable of inducing tumor cell oncosis, a preparation method and application thereof. The nanoliposomes are constructed by inserting ascorbyl palmitate as a lipid component into a phospholipid bilayer, and are prepared by a film dispersion method from lecithin, cholesterol, pegylated phospholipid and ascorbyl palmitate. The average particle size of the nanoliposomes is 80-150 nm, the encapsulation rate is high, and the stability is good. The nanoliposomes can be administered through a tail vein, utilize the specific hydrolysis of matrix metalloproteinase MMP-2 which is highly expressed by tumor cells to hydrolyze ascorbyl palmitate, release palmitic acid and ascorbic acid, thereby inducing Caspase-independent oncosis of tumor cells, effectively overcoming apoptosis-related drug resistance, and reversing a tumor immunosuppressive microenvironment. The nanoliposomes avoid the cumulative toxicity of metal-type inducers, and provide a new safe and effective option for reversing chemotherapy resistance.

The invention discloses a Weissella sinus strain as well as a fermentation product filtrate and a biological product thereof, and belongs to the technical field of cosmetics. The strain can be used in the field of cosmetics, has excellent HAS inhibition capability, also has remarkable DPPH and ABTS free radical scavenging capability, and can realize a good antioxidant effect; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of various matrix metalloproteinases can be remarkably inhibited, and a certain effect of reducing cell senescence markers is achieved; in addition, the fermentation product filtrate can inhibit the activity of tyrosinase in vitro, can also significantly inhibit the generation of melanin in a zebra fish model, and has the potential of being used as a tyrosinase inhibitor or a melanin generation inhibitor; the fermentation product filtrate can also significantly inhibit the secretion amount of a cell inflammatory factor NO, has a good soothing effect, and has a certain ability to regulate the skin micro-ecology.

The invention discloses a flora-friendly selective antifungal peptide HRI and application thereof, and belongs to the technical field of novel antibacterial materials. The antifungal peptide HRI provided by the invention can only selectively resist pathogenic fungi instead of bacteria and especially does not resist symbiotic bacteria on the premise of maintaining flora balance, has relatively high biological safety, and has a certain inhibition effect on matrix metalloproteinase (MMP-9).

This invention provides a highly stable recombinant type XVII collagen, its construction method, and its applications, belonging to the field of bioengineering technology. The invention selects the amino acid sequence of natural human type XVII collagen as the basic unit and repeatedly replicates it to obtain recombinant type XVII collagen. The design process of the recombinant type XVII collagen avoids unstable GXY triplets, eliminates potential matrix metalloproteinase cleavage sites and glycosylation sites, and retains the KGD active sequence, thus reducing the probability of containing easily degraded sites. The sequence fragment of the recombinant type XVII collagen is derived from the full-length sequence of the natural collagen gene, exhibiting excellent solubility, bioactivity, and high stability. It remains stable under high-temperature storage conditions and without alkaline conditions, making it suitable as a raw material for the production of pharmaceuticals, medical devices, biomaterials, or cosmetics, demonstrating significant practicality.

The application relates to the technical field of genetic engineering, in particular to a sucrose phosphorylase mutant and application thereof in synthesis of alpha-maronia. Streptococcus parasanguinis The application is characterized in that the histidine at the 152th position of the wild-type sucrose phosphorylase from Streptococcus sanguis is mutated into threonine, and the isoleucine at the 336th position is mutated into proline, so that the sucrose phosphorylase mutant is obtained, the catalytic activity and stability of the sucrose phosphorylase are significantly improved. When sucrose and hydroquinone are used as substrates, the yield of alpha-maronia is increased by more than 40% compared with the wild type, and the catalytic reaction time can be greatly shortened.

The application discloses oleanane triterpene saponin compounds with improved mitochondrial energy anti-aging, a preparation method and application thereof, and belongs to the technical field of medicines and cosmetics. Through systematic chemical component research on Zhejiang Hongshan tea flowers, two new oleanane triterpene saponin compounds CK and CL are separated, and the chemical structures are determined by using high-resolution mass spectrometry and nuclear magnetic technology. In-vitro anti-photoaging activity research shows that the new compounds CK and CL provided in the application have good protection effects on ultraviolet-induced fibroblast photoaging, can significantly improve the content of type I collagen, reduce the expression of matrix metalloproteinases mmp1 and mmp3 , increase the generation of mitochondrial autophagosomes and lysosomes, promote mitochondrial autophagy, relieve mitochondrial damage and improve cell senescence. The preparation method provided in the application is simple, and the activity is significant, so that the application is expected to be developed into a new anti-skin aging medicine or cosmetic.

The invention provides a stem cell composition for treating male erectile dysfunction and a preparation method thereof, and belongs to the technical field of medicines. Adding the magnetic particles loaded with the platelet-derived growth factors and the matrix metalloproteinase into a culture medium, activating the amniotic mesenchymal stem cells, and separating to obtain the activated amniotic mesenchymal stem cells, paracrine compositions and magnetic nutrient particles, and mixing the stem cells with an intercalation sustained-release drug compound and a penetration enhancing and activating agent, and adding the mixture into a temperature-sensitive hydrogel sustained-release system to prepare the stem cell composition for treating male erectile dysfunction. The stem cell composition for treating male erectile dysfunction plays a role through multiple ways, promotes angiogenesis, improves penis hemodynamics, inhibits smooth muscle cell apoptosis, adjusts neurological functions, enhances endothelial progenitor cell recruitment and the like, so that damaged penis tissues are more comprehensively repaired and regenerated, and the male erectile dysfunction treatment effect is improved. Wide application prospects are realized.

This invention provides the application of costunolide in the preparation of a drug for preventing posterior capsule opacification. Costunolide effectively reduces posterior capsule opacification after extracapsular lens extraction (ECLE) and exhibits significant anti-inflammatory and anti-fibrotic effects in both in vitro and in vivo models. Mechanistic studies have identified matrix metalloproteinase 8 (MMP8) as a key downstream effector of costunolide, confirming that costunolide inhibits MMP8 expression, thereby blocking the transformation of lens epithelial cells (LECs) into mesenchymal cells and subsequent fibrotic processes. This lays a solid foundation for developing targeted therapies for posterior cataract, a common surgical complication.

Disclosed is an in vitro method for assessing whether a human subject has periodontitis. The method comprises detecting, in a sample of saliva from said subject, the concentrations of the proteins Hepatocyte Growth Factor (HGF) and Matrix Metalloproteinase 8 (MMP-8). Based on the concentrations determined, and adding age, and possibly other demographic markers such as sex and / or BMI, a testing value reflecting the joint concentrations is determined for said proteins, in combination with one or more demographic markers. The testing value is compared with a threshold value. The threshold reflects in the same manner the joint concentrations and the age, and possibly other demographic markers, as associated with periodontitis and may be seen as an upper limit of testing values as seen in a population of subjects without periodontitis. Thereby a testing value at or above the threshold value is indicative for periodontitis in said subject.

The invention discloses a functionalized acellular matrix material and a preparation method thereof, and relates to the technical field of biomedical treatment, the functionalized acellular matrix material comprises: a composite ECM skeleton, which is prepared by compounding squid sheaths and amniotic membranes and performing acellular treatment; the multi-stage response degradation switch comprises a matrix metalloproteinase sensitive peptide cross-linking bridge and a polythioether-polylactic acid block copolymer oxidation response chain segment; the natural biological conductive network is formed by compounding an in-situ polymerization product of a mussel byssus protein conductive fragment and amino-modified graphene quantum dots, the characteristics of squid sheaths and amniotic membranes are fused to construct a composite ECM skeleton, a multi-stage response degradation switch is integrated, and the comprehensive performance of the material is optimized; meanwhile, a natural biological conductive network is introduced, so that the electroactivity is improved, and a safe, efficient and powerful novel stent solution is provided for tissue engineering.

This invention relates to a ZIF-8-based composite nanoparticle with a core-shell structure. The core is a zeolite imidazole ester backbone ZIF-8 carrier, and the pores of the ZIF-8 core are loaded with a photothermal agent. Liposomes are coated on the outer surface of the core as a shell, and CD90 antibodies are coupled to the surface of the liposomes. The composite nanoparticle targets mesenchymal stem cells, thereby more effectively delivering zinc ions into mesenchymal stem cells (MSCs) to regulate MMP-10 (matrix metalloproteinase-10) expression. The composite nanoparticle can be used to prepare drugs or medical dressings for treating bone defects and / or promoting vascularized bone regeneration.

The invention relates to a recombinant protein, printing ink, a 4D scaffold and a preparation method and application thereof, and belongs to the technical field of biology. The recombinant protein provided by the invention can provide extracellular matrix-like structure support, catalyzes generation of dopamine groups, endows the recombinant protein with bionic adhesion performance, and meanwhile can regulate and control the activity of matrix metalloproteinase, maintain balance of scaffold degradation and tissue regeneration, target inflammatory factor genes and relieve local inflammatory response. The recombinant protein, the P (NIPAM-co-AA) copolymer and the KLT peptide-loaded mesoporous SiO2 jointly form the printing ink, and the printing ink can be used for printing a 4D biological scaffold, not only has the effect of the recombinant protein, but also has the effects of temperature responsiveness and promoting cell proliferation and vascularization. Meanwhile, due to the fact that a TPMS structure model is adopted in the 4D stent, directional curling can be achieved, and the better mechanical property is achieved; the hydrogel can be used for tissue repair and filling, tissue regeneration is promoted, and wounds are rapidly healed.