185 results about "Elastin" patented technology

The invention belongs to the technical field of biomedical tissue engineering materials, and particularly relates to injectable composite plastic gel and a preparation method thereof. The injectable plastic gel provided by the invention is formed by suspending and compounding the cross-linked sodium hyaluronate gel particles and the biological tissue extracellular matrix particles, the two material particles in the injectable composite plastic gel are uniform in size and controllable in size, other toxic catalysts do not need to be added in the preparation process, and the injectable plastic gel is easy to prepare. The collagen / elastin composite material is rich in collagen and elastin, and shows good biomechanical properties. Meanwhile, the composite plastic gel contains a plurality of bioactive factors, and after the composite plastic gel is injected and filled into a body, the composite plastic gel is slowly degraded and can promote the generation and ingrowth of autologous fat cells and microvessels, so that the balance of degradation and remodeling is achieved. The preparation method of the injectable plastic gel provided by the invention can be widely applied to the fields of plastic surgery, medical beauty and the like.

The invention relates to the technical field of gel separation and extraction, in particular to a precipitation and separation device for elastin gel preparation, which comprises a precipitation cylinder, a liquid outlet is arranged above the circumferential side wall of the precipitation cylinder, and a cleaning ring for cleaning attached gel is arranged in the precipitation cylinder. A cleaning ring is arranged in the precipitation cylinder, a filtering mechanism for intercepting gel is arranged at the center of the cleaning ring, the filtering mechanism is rotatably connected with the cleaning ring, and a driving mechanism for driving the cleaning ring to slide up and down is arranged at the top end of the cleaning ring. The cleaning ring is arranged on the bottom of the precipitation cylinder, elastin gel attached to the inner wall of the precipitation cylinder is effectively scraped, meanwhile, the filtering mechanism moves downwards along with the cleaning ring, the water-phase solution is allowed to penetrate through the filtering mechanism to intercept gel particles, floating and floating of the gel particles in the water-phase solution are avoided, it is ensured that the gel particles can be smoothly precipitated to the bottom of a container, and the recovery efficiency of the gel is improved.

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

The invention relates to a recombinant elastin and a preparation method thereof as well as a recombinant elastin raw material and application thereof, and belongs to the technical field of recombinant proteins. The amino acid sequence of the recombinant elastin is an amino acid sequence as shown in SEQ ID No.1 or a derivative amino acid sequence of which the sequence identity with the SEQ ID No.1 is more than 90%. The protein is high in expression level, high in purity and relatively high in biological activity, and has the effects of supplementing skin collagen, resisting aging and repairing skin barriers.

The invention provides a pine pollen fermentation product with anti-wrinkle and anti-aging functions as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The pine pollen extract is prepared by fermenting lactobacillus plantarum, the total polysaccharide content of the obtained pine pollen fermentation product is maximally increased by 77.3%, the FRAP total antioxidant capacity is maximally increased by 2.85 times compared with that of the unfermented pine pollen fermentation product, the anti-wrinkle effect of human skin cells can be effectively improved, and the pine pollen extract has a relatively strong proliferation effect on an ultraviolet damage model of human skin fibroblasts; the pine pollen extract has the advantages that the pine pollen extract is fermented, the elastin content is remarkably increased, the life of nematodes is effectively prolonged, lipofuscin deposition is reduced, and compared with unfermented pine pollen extract, the anti-aging capacity of the pine pollen can be effectively improved; the preparation method provided by the invention is low in price and simple in process, and the active ingredients and the efficacy of the traditional Chinese medicine composition are improved in a simplest and effective manner.

The invention discloses a preparation method of vascular tissue-derived extracellular vesicles, and belongs to the field of cytobiology. According to the preparation method of the extracellular vesicles, the artery blood vessel tissue is directly subjected to enzymolysis through collagenase, the extracellular vesicles are obtained by combining low-speed centrifugation and ultra-speed centrifugation methods and utilizing a differential centrifugation mode, and the technical blank of obtaining the extracellular vesicles from the blood vessel tissue is filled. Experimental results show that the extracellular vesicles can be successfully prepared from collagenase I, collagenase II, collagenase IV, collagenase V or collagenase IV combined with elastin, the prepared extracellular vesicles are different in particle size, form, yield, protein quantity, purity and the like, and the quality of the extracellular vesicles obtained by enzymolysis of collagenase IV combined with elastin is optimal. The extracellular vesicles are extracted from vascular tissues with normal physiological characteristics for the first time, a new source is provided for preparation of the extracellular vesicles, and the extracellular vesicles have great significance in expanding application of the extracellular vesicles.

The present invention relates to a hydrogel-based drug delivery system that can efficiently deliver the required amount of a drug to a desired location for a longer period of time. The hydrogel-based drug delivery system includes: an amphipathic block copolymer including a mixture of a first poloxamer and a second poloxamer that have different molecular weights; and a protein extract (containing elastin and collagen. A drug delivery composition for promoting tissue regeneration, according to the present invention, is mixed with a drug and introduced into the body, thus increasing the excretion time of the drug in the body, has phase transition properties of a liquid (sol) state at room temperature while being gelled at body temperature, thus being convenient to inject as an injectable agent, can be applied to various body parts.

The application discloses a kind of elastin-like polypeptide V 20 K 40 L, mRNA vaccine and preparation method and application in preparation tumor prevention, mitigation or treating drug.The elastin-like polypeptide carrier V 20 K 40 L provided by the application has good biocompatibility, and has no adverse reaction in vivo.The elastin-like polypeptide V 20 K 40 L provided by the application has amphiphilic structure, can be loaded Melan-A mRNA by hydrophobic self-assembly and electrostatic adsorption, and is in the state of shrinkage when higher than Tt temperature, which can prevent the uncontrollable leakage of mRNA.Furthermore, V 20 K 40 L contains lysosome escape peptide, which can further improve the transfection efficiency of mRNA by promoting lysosome escape.The elastin-like polypeptide V 20 K 40 L in the application can deliver Melan-A mRNA efficiently and safely, and realize the prevention and treatment of melanoma.

The invention provides a pedicel and inflorescence extract ZTM100 of Gastrodia elata Blume, a preparation method of the pedicel and inflorescence extract ZTM100 and application of the pedicel and inflorescence extract ZTM100 in the technical field of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The ZTM100 (100 [mu] g / mL) provided by the invention can significantly improve the expression quantity of human dermal fibroblast elastin gene Elastin under UVA radiation, and does not show obvious cytotoxicity to human fibroblasts in a concentration range of 800 [mu] g / mL. The ZTM100 can be used for preparing medicines or cosmetics for resisting skin aging caused by UVA, reducing scar formation, repairing skin injury and repairing wounds.

A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes and method of manufacture. A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes, comprising: a first layer of ADM of biological origin arranged inferiorly to delimit a cavity with a lower edge; a filling material disposed in the cavity surrounded by the first layer of ADM consisting of a ground ADM having the consistency of a fibrous paste; and a second layer of ADM of biological origin arranged on the upper part covering the filling material and whose upper edge rests peripherally on the lower edge of the first layer of ADM, thus defining a rim;where the first layer of ADM and the second layer of ADM are joined by an eyebrow overlap along the upper edge of the first layer of ADM with the lower edge of the peripheral rim using a biocompatible adhesive, or by absorbable suture; where the first layer of ADM, the second layer of ADM and the ADM filling material are not immunogenic; and where the ADM comprises collagen fibers, fibronectin, elastin, laminin, glycosaminoglycans and hyaluronic acid. Method for its manufacture.

The invention discloses a blood vessel cross-linking agent and a medical device, and relates to the technical field of blood vessel treatment. The blood vessel cross-linking agent comprises a photosensitizer and an anti-inflammatory composition, and the mass ratio of the photosensitizer to the anti-inflammatory composition is (1: 0.02)-(1: 100); wherein the anti-inflammatory composition comprises an anti-inflammatory drug and a temperature-sensitive hydrogel, and the mass ratio of the anti-inflammatory drug to the temperature-sensitive hydrogel is (1: 1)-(1: 100). According to the blood vessel cross-linking agent disclosed by the invention, collagen and elastin in a blood vessel wall can be induced to be rapidly combined and form a stent in situ by exciting the photosensitizer, so that healing and repairing of blood vessels are realized; the anti-inflammatory composition disclosed by the invention comprises the anti-inflammatory drug and the temperature-sensitive hydrogel, also has the effects of durably resisting inflammation and eliminating side effects of active oxygen, and also can reduce the risk of degradation of the anti-inflammatory drug under illumination and ensure the activity and concentration of the anti-inflammatory drug.

The present invention provides a pharmaceutical composition comprising an exosome encapsulating one or more cargo RNAs containing package RNA and one or more fusion proteins, wherein the nucleotide sequence of the package RNA is at least about 80%, about 85%, about 90%, about 95%, or about 100% identical to the nucleotide sequence of an elastin (ELN) gene or elastin messenger RNA (mRNA) also containing any isoform. Additionally, the present invention provides a method for treating an elastin deficiency-related disease in a subject using the pharmaceutical composition of the present invention.

The invention belongs to the field of chemical synthesis, and particularly relates to a chemical synthesis method of elastin-like polypeptide. The invention provides a chemical synthesis method of elastin-like, which comprises the following steps of: constructing a pentapeptide repetitive sequence (valine-proline-glycine-X-glycine, X is amino acid except proline, and the amino acid except glycine is respectively and independently selected from levoamino acid or dextroamino acid) of elastin-like polypeptide through liquid phase reaction; the elastin-like polypeptide can be prepared with the target molecular weight of 1000-500000 by adding amino groups and carboxyl groups into an organic solvent, forming orthogonal protection on the amino groups and the carboxyl groups, then selectively removing amino or carboxyl end protecting groups respectively, accurately coupling the amino groups and the carboxyl groups in a solution and repeating the deprotection and coupling processes for multiple times. The preparation method can realize accurate regulation and control of the molecular weight, sequence, chirality and topological structure of the elastin-like protein, improves the synthesis efficiency of the high-molecular-weight elastin-like polypeptide, has the advantages of large synthesis scale, low cost and the like, and has a wide application prospect.

This invention discloses a polypeptide with anti-wrinkle and oil-controlling effects, belonging to the field of cosmetic polypeptide technology. The polypeptide is heptapeptide-6, with the amino acid sequence Asn-Asp-Glu-Gly-Leu-Tyr-Leu. At concentrations of 0.001 mg / mL to 0.1 mg / mL, heptapeptide-6 significantly promotes the synthesis of type I collagen and elastin by human skin fibroblasts, exhibiting excellent anti-wrinkle and firming activity. At concentrations of 1 ppm to 50 ppm, it significantly inhibits lipid accumulation in *C. elegans* and lipid expression in zebrafish, with a maximum lipid inhibition rate of 24.38%, and also demonstrates clear oil-controlling activity. This invention's polypeptide has a single structure, overcoming the shortcomings of existing skincare polypeptides with single efficacy and complex formulations. It features stable processing, high safety, and is suitable as a core active ingredient in anti-wrinkle and oil-controlling cosmetics, providing a new avenue for the development and application of multifunctional skincare polypeptides.

This invention discloses a layered anti-aging composition and its application. It comprises 4-10 parts of *Vigna angularis* fruit extract, 0.01-0.2 parts of soluble collagen, and 10-20 parts of *Fucus vesiculosus* extract, all parts by weight. The composition of this invention employs a layered anti-aging skincare strategy ("layered" generally refers to different skin areas, i.e., "cellular layer-dermis-epidermal layer"). This composition can promote the expression of LOR (lower retinal occlusion) and the relative expression level of TGM5 (epidermis), increase the content of type I procollagen C-terminal peptide and elastin (dermis), enhance mitochondrial activity, and increase ATP content (cellular layer). That is, it works progressively from the epidermis to the dermis to the cellular layer, having a relevant effect on each layer of skin, and possessing the functions of enhancing skin elasticity, firming, anti-aging, and repairing the skin barrier.

The present invention relates to a dispersion comprising acellular dermal matrix microparticles, a filler comprising the same, and uses thereof. More specifically, the present invention relates to a filler comprising a dispersion containing acellular dermal matrix microparticles obtained by supercritical fluid extraction and high pressure dispersion operations, and a method for preparing the same. The acellular dermal filler according to the present invention has excellent biological stability and biocompatibility, and extracellular matrix components such as collagen and elastin are significantly preserved therein. In addition, the filling agent also shows excellent biochemical stability, the pH value or the collagen content and the elastin content are kept unchanged in the storage process, and the filling agent also shows physical stability and can continuously maintain injectability. Therefore, the acellular dermal filler can be effectively used as an injectable filler for repair or volume expansion of soft tissue and reduction of wrinkles in the field of plastic and regenerative medicine.

This invention relates to a sustained-release eye drop and its preparation method, belonging to the field of ophthalmic drug technology. The invention provides a self-assembled microparticle comprising an elastin-like peptide and sodium hyaluronate; the molar ratio of the elastin-like peptide to sodium hyaluronate is 5-7:1-2; the amino acid sequence of the elastin-like peptide is shown in SEQ ID NO.1. The self-assembled microparticles of this invention have a sustained-release effect and exhibit synergistic properties with hydroxypropyl methylcellulose. The eye drops prepared by combining these two components possess excellent sustained-release performance, adhesion, and stability, allowing for prolonged application to the eye, effectively solving the problem of short duration of action in existing eye drops.

The invention discloses a preparation method and application of elastin nano-microsphere liposome, and belongs to the technical field of biology. According to the invention, epsilon-polylysine and elastin are selected to be combined, and the epsilon-polylysine and the elastin can be synergistically driven to be self-assembled through electrostatic interaction, hydrophobic interaction and hydrogen bonds to form stable elastin nano-microspheres; the formed elastin nano-microspheres are beneficial to liposome encapsulation, so that the encapsulation efficiency of the liposome is improved; by utilizing the bacteriostatic activity of epsilon-polylysine, the invasion of microorganisms to elastin is relieved; and the transdermal efficiency is improved. The liposome disclosed by the invention can be used for encapsulating various elastin nano microspheres, particularly self-assembled elastin which is used in the invention and has a function of promoting cell proliferation and is shown in SEQ ID NO.1, and the elastin nano microsphere liposome is prepared from the self-assembled elastin, so that the application of the self-assembled elastin in the field of cosmetics is facilitated.

The application belongs to the technical field of cosmetics and skin care, and provides a polypeptide compound combination for strengthening the resistance to free radicals for skin anti-aging and tightening. The application constructs a multi-pathway efficacy system with five active polypeptides as the core, which respectively covers free radical scavenging, stress resistance, anti-glycation / anti-carbonylation, collagen promotion and repair promotion nodes. In the obtained in-vitro and 3D full-thickness skin model data, the product shows multiple synergistic effects such as antioxidant, anti-carbonylation, anti-glycation, collagen promotion, elastin promotion and repair promotion, and can be used in various types and functions of cosmetics.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

An object of the present invention is to prepare a novel food-derived exosome composition and to provide a cancer inhibitor or an agent for preventing decrease in tissue elasticity or an agent for improving elasticity containing a novel active ingredient. As a result of intensive studies on the purification process, the inventors have found that an exosome can be extracted from yeast mash and moromi by a unique production method. The inventors have found that the exosome recovered from the yeast mash and the moromi has a cancer cell proliferation inhibition ability, an ability to promote increase in the amount of granzyme, and an ability to promote expression of collagen and elastin. Accordingly, the inventors provide a composition for treating or preventing cancer and an agent for preventing decrease in tissue elasticity or an agent for improving elasticity, containing exosomes derived from yeast mash and moromi of sake.

This invention belongs to the field of cosmetic technology, specifically relating to an anti-aging composition containing lactoferrin and its application. The anti-aging composition provided by this invention, through scientific compounding, achieves synergistic effects in anti-aging among four components: medicinal Fomitopsis thunbergii extract, butterfly pea flower extract, Artemisia annua extract, and lactoferrin. This results in excellent elastase inhibition, preventing the degradation of elastin in the dermis and effectively strengthening the elastin scaffold, thereby enhancing the anti-aging effect of the composition. When applied to a prepared emulsion, this anti-aging composition can improve skin firmness (R0 change rate), skin elasticity (R2 change rate), and facial wrinkles (wrinkle change rate), exhibiting anti-aging effects of improving wrinkle reduction and firmness, and promoting skin elasticity. Furthermore, it does not cause skin irritation, possessing safe and gentle characteristics, a wide range of applications, and high consumer acceptance.

The invention discloses application of cyclic peptide or salt thereof in cosmetics, and belongs to the technical field of cosmetic peptides, the natural cyclic peptide comprises alanine (Ala), glycine (Gly), proline (Pro), valine (Val) and tyrosine (Tyr), and is a compound with the following formula I. The invention further discloses a preparation method of the natural cyclic peptide. The cyclopeptide compound disclosed by the invention has the effects of promoting generation of type I collagen and elastin, inhibiting generation of matrix metalloproteinase I (MMP-1) under UVA irradiation and preventing collagen degradation, so that the cyclopeptide compound has the effects of resisting wrinkles, tightening skin and the like.

The invention belongs to the technical field of cosmetics, and discloses a composition with tightening, moisturizing and repairing effects as well as a preparation method and application thereof. The composition is prepared from the following components in parts by mass: 1 to 30 parts of elastin, 1 to 30 parts of fibronectin, 1 to 40 parts of milk protein and 1 to 35 parts of agilawood extract. The composition disclosed by the invention is mild and non-irritant, is good in formula stability, and has multiple skin care effects of tightening, moisturizing and repairing, so that the composition has a wide application prospect in the development of cosmetics.

This invention belongs to the technical field of 3D printing of biological tissues and organs, and discloses a biomimetic valve multi-nozzle 3D bioprinted scaffold and its application. The scaffold, from bottom to top, comprises a firmly connected elastin layer, a polysaccharide layer, and a collagen fiber layer. Each layer is constructed by alternating 3D printing of at least two materials; one of these materials is a photocurable hydrogel material providing mechanical support, and the other is a photocurable hydrogel material mixed with bioactive substances. Furthermore, the bioactive substances used in the elastin layer, polysaccharide layer, and collagen fiber layer are different from each other. This invention improves the structure and composition of the 3D bioprinted scaffold, creating a biomimetic three-layer structure and composition for a valve. The scaffold, designed with a three-layer structure of elastin, polysaccharide, and collagen fiber layers, provides cells with a specific sensory microenvironment, stimulating them to secrete corresponding extracellular matrix and completing extracellular matrix remodeling.

The invention belongs to the technical field of protein engineering, and discloses a humanized collagen XVII type polypeptide as well as a preparation method and application thereof. The amino acid sequence of the humanized collagen XVII type polypeptide is as shown in SEQ ID NO.1, and the polynucleotide sequence for coding the humanized collagen XVII type polypeptide is as shown in SEQ ID NO.2. The invention also provides application of the polypeptide in preparation of a protein product with functions of preventing hair loss and growing hair and / or repairing, resisting wrinkles and tightening skin. The application proves that the humanized collagen XVII type polypeptide can promote proliferation of hair papilla cells, keratinocytes and fibroblasts and promote up-regulation of expression of beta-Catenin signal channel protein closely related to hair growth in the hair papilla cells; the up-regulation expression of fibroblast type III collagen, type IV collagen, type VII collagen, fibronectin and elastin is promoted, so that the polypeptide is proved to have the effects of preventing hair loss and growing hair and / or repairing, resisting wrinkles and tightening skin.

The invention relates to the technical field of collagen, in particular to expression and application of recombinant humanized collagen. The recombinant collagen comprises AXBYCZ (AXBYCZ); wherein X and Z are independently selected from 0 or 1, and Y is an integer greater than or equal to 1; x and Z are not 0 at the same time; the structural domain A is a collagen N-terminal peptide or a peptide fragment thereof, the structural domain B is a Gly-Xaa-Yaa structure, and the structural domain C is a collagen C-terminal peptide or a peptide fragment thereof; the recombinant collagen not only shows excellent cell compatibility including relatively high cell viability, cell adhesion rate and cell proliferation rate, but also has the effects of resisting inflammation and inhibiting melanogenesis, and can promote synthesis of type I collagen, type III collagen and elastin, so that the recombinant collagen can be used for preparing the anti-inflammatory and anti-melanogenesis collagen. The method can be widely applied to the technical fields of biological medicine, medical equipment, cosmetics and biological engineering.

The present application relates to the technical field of wound care, and in particular to a responsive release microcapsule and a preparation method and application thereof.The preparation method comprises the following steps: S1, dissolving polylactic acid-glycolic acid copolymer in an organic solvent, adding an emulsifier, and ultrasonic emulsification to form a water-in-oil emulsion; S2, mixing a polyphenol compound, elastin, vegetable fat and a probiotic bacteria suspension to form a four-component mixed solution, and slowly adding the four-component mixed solution to the water-in-oil emulsion, continuing ultrasonic emulsification to form a multiple emulsion, evaporating and removing the organic solvent to obtain a four-component active ingredient mixed solution; S3, dissolving PDMAEMA in deionized water, adding a crosslinking agent and stirring, slowly adding the four-component active ingredient mixed solution, crosslinking reaction and freeze-drying to obtain the responsive release microcapsule.The microcapsule can release corresponding active ingredients according to the pH value change in different stages of wound healing, effectively shorten the wound healing time, improve the healing quality, and reduce scar formation.

The invention provides a safe and efficient animal skin biological degreasing method. The method is characterized in that esterase or protease with weak affinity to collagen and elastin and strong affinity to fat cell membranes is selected for synergistic effect, on the basis of not excessively damaging collagen and elastin, the permeation efficiency of lipase in animal skin and the full contact efficiency of lipase and grease are improved, and the catalytic effect of lipase is improved. And then through the synergistic effect of multiple lipases-alkalis, the hydrolysis rate of the grease is increased, and hydrolysates are effectively removed, so that efficient degreasing is realized. Through the technology, the grease content of the animal grease can be reduced to 2% or below, a petroleum-based surfactant is not used, and good energy-saving and emission-reducing effects are achieved. The method has important guiding significance for developing animal skin processing biotechnology and complex enzyme preparations.

According to the composition, hydrolyzed collagen, soluble collagen and elastin serve as core repairing components, active components such as a bifidus yeast fermentation product lysate, rosa damascena flower water and a centella asiatica extract are compounded, and auxiliary components such as 1, 2-hexanediol are matched, so that the collagen repairing composition is prepared. And a synergistic effect is realized through scientific proportioning. The composition disclosed by the invention can be quickly absorbed by skin, effectively replenishes skin moisture, locks moisture, improves skin dryness, cares skin barrier and makes skin soft, moist and glossy; the preparation method is simple in process, high in stability and suitable for industrial production.