19 results about "Autologous fat" patented technology

The invention provides an autologous fat-derived mesenchymal stem cell composition as well as a preparation method and application thereof, and belongs to the technical field of medical auxiliary materials. The method comprises the following steps: separating mesenchymal stem cells from autologous fat particles, culturing in a stem cell culture medium added with cell stimulation particles and fetal calf serum, separating to obtain stem cells and an extracellular matrix, preparing the stem cells into stem cell lipidosome, and preparing the stem cell lipidosome into the mesenchymal stem cells. Sanguinarine and curcumin are embedded in a composite shell material of chitosan and beta-cyclodextrin to prepare the sustained release microspheres. The mesenchymal stem cell composition of the autologous fat source prepared by the invention has good air permeability, softness, certain mechanical property and good biocompatibility, can quickly promote wound healing, is convenient to remove, and does not cause secondary injury.

The invention discloses a monitoring system for fat necrosis risk after fat transplantation, and belongs to the technical field of autologous fat transplantation. The fat necrosis risk monitoring system comprises an oxygen saturation detection device used for obtaining oxygen saturation information of a transplantation area and a contrast area; the temperature detection device is used for acquiring temperature information of the transplantation area and the contrast area; the pressure detection device is used for acquiring pressure information of the transplantation area and the contrast area; the data acquisition device is electrically connected to the oxygen saturation detection device, the temperature detection device and the pressure detection device and is used for acquiring information detected by the oxygen saturation detection device, the temperature detection device and the pressure detection device; and the upper computer is electrically connected to the data acquisition device and is used for acquiring the data acquired by the data acquisition device and processing the acquired data. According to the invention, the fat necrosis comprehensive risk coefficient of the transplantation area can be obtained, so that an accurate quantitative index is provided for evaluation and nursing work after fat transplantation.

The invention discloses a medical fat collection device with separation and purification functions, and relates to the technical field of fat extraction and collection, the medical fat collection device comprises an absorption gun and a collection assembly, a connecting pipe is arranged between the absorption gun and the collection assembly, the absorption gun and the collection assembly are connected with a negative pressure pump, and the negative pressure pump is started; by means of the absorption gun, autologous fat is absorbed through the absorption gun, fat mixed liquid is primarily separated in the absorption gun, fat and most blood are separated, the primarily separated fat is conveyed into the collection assembly through the connection pipe by the absorption gun, and after the fat is conveyed to the collection assembly, the fat is separated again in the collection assembly. The fat is further purified, residual blood water in the fat is separated, so that the fat separation and extraction effect is achieved, the rejection problem caused by fat transplantation is avoided by sucking the fat, treating the fat and then transplanting the fat to other parts, and the quality of plastic surgery medicine and wound repair medicine surgery is improved.

The invention relates to the technical field of cell culture, in particular to a device and method for preparing platelet-rich plasma and autologous fat gum composite graft material.The device comprises a touch console and a base which are vertically arranged at intervals, and a pair of sampling centrifugal parts and a pair of mixing parts are symmetrically arranged between the touch console and the base; the pair of sampling centrifugal parts are used for respectively and independently sampling autologous fat and venous blood, and the pair of mixing parts are used for mixing purified fat and platelet-rich plasma to form the composite graft material. By means of the closed pipeline system integrating the liposuction module, the centrifugation module and the mixing module, the whole-process isolation operation from collection to mixing of fat and blood is completed, and the effect of reducing the infection risk is achieved; a liquid layering interface after centrifugation is monitored in real time through a laser sensor, an ARM main control module is combined to dynamically control opening and closing of an electromagnetic valve, automatic separation of middle-layer purified fat and upper-layer PRP is completed, and the effects of automatic fat recovery and platelet plasma recovery are achieved.

A system for automatically completing the enrichment and cleaning of fat matrix components, which includes a transmission path 1 from a buffer container to a fat processing structure, which is composed of an output pipeline 3, a main pipeline and an input pipeline 1; a transmission path 2 from the fat processing structure to a waste liquid collection container, which is composed of an output pipeline 1, a main pipeline and an input pipeline 3; a transmission path 3 from the fat processing structure to a centrifugal enrichment structure, which is composed of an output pipeline 1, a main pipeline and an input pipeline 2; a transmission path 4 from the centrifugal enrichment structure to a waste liquid collection container, which is composed of an output pipeline 2, a main pipeline and an input pipeline 3; and a transmission path 5 from a buffer container to a centrifugal enrichment structure, which is composed of an output pipeline 3, a main pipeline and an input pipeline 2. Each transmission path uses a power pump to provide energy transmission force at different times to ensure the completion of the processing after the autologous fat is extracted, and finally complete the enrichment and cleaning of fat matrix components.

The specification relates to a method of manufacturing a diabetic foot patient-specific skin regeneration sheet, and a diabetic foot patient-specific skin regeneration sheet. The method includes extracting autologous adipose tissue, removing fibers in the autologous adipose tissue, crushing the autologous adipose tissue, and acquiring an adipose tissue extract by filtrating the crushed autologous adipose tissue.

The present invention provides a composition of mesenchymal stem cells derived from autologous fat, a preparation method thereof, and an application thereof, belonging to the technical field of medical adjuvants. Mesenchymal stem cells are isolated from autologous fat particles and cultured in a stem cell culture medium supplemented with cell stimulating particles and fetal bovine serum. Stem cells and extracellular matrix are obtained by separation. The stem cells are prepared into stem cell liposomes, and sanguinarine and curcumin are embedded in a composite shell material of chitosan and β-cyclodextrin to prepare sustained-release microspheres. The composition of mesenchymal stem cells derived from autologous fat prepared by the present invention has good air permeability, softness, certain mechanical properties and good biocompatibility, can rapidly promote wound healing, is convenient to remove, and will not cause secondary damage.

The application discloses a kind of composite protein gels and preparation method thereof, its preparation method includes the following steps: with the blood collection tube with anticoagulant is collected blood;The above-mentioned blood is centrifuged at 300-700g centrifugal acceleration at room temperature 10-20min;After centrifugation, blood is transferred to biological safety cabinet, and the upper plasma layer is extracted, and then it is placed in-80 ℃ ultra-low temperature refrigerator and rapidly frozen, and the freezing time is 24-72h;Then, the frozen plasma is taken out, and freeze-drying treatment is carried out under the condition that the temperature is-55-65 ℃ and the pressure is 50-100Pa, and the freeze-dried plasma is prepared;Calcium chloride injection is added to the freeze-dried plasma at a volume ratio of 1:0.5-2, then reacted in a water bath at 60-90 ℃ for 5-15min, and removed to prepare.The protein gel can effectively solve the problem of easy skin stiffening, collapse, high in vivo absorption rate and poor filler stability caused by hyaluronic acid and autologous fat.

The utility model discloses an autologous fat component separation filler, and relates to the technical field of fat filling. Comprising a main body, the main body is composed of a push-injection cylinder and a filling cylinder, the lower inner wall of the push-injection cylinder is fixedly sleeved with a sealing plate, one end, away from the sealing plate, of the push-injection cylinder is rotatably provided with a push-injection knob, the surface of the push-injection knob is sleeved with a lead screw in a penetrating and threaded mode, and a push rod is fixedly installed at the end of the lead screw and slides at the bottom of the sealing plate in a limited mode; a piston head is fixedly installed at the end, away from the lead screw, of the push rod and sleeved with the inner wall of the filling cylinder. The push injection knob is rotated to drive the screw rod in threaded engagement with the push injection knob to move up and down, and in the process, the sliding groove slidably sleeves the surface of the convex block to play a limiting role, so that the piston head makes piston motion on the inner wall of the filling cylinder, constant-speed separation and filling of autologous fat are achieved, and the filling speed can be accurately controlled; therefore, the survival rate of fat cell transplantation is improved.

The present invention relates to a fat processing device, specifically a fat processing device for autologous reinjection in aesthetic and reconstructive medicine. The fat processing device comprising a container, a lid, a filter element and an impeller.

The utility model discloses an autologous fat transplantation needle, and relates to the technical field of transplantation needles. The end, away from the connecting piece, of the needle tube is of a conical structure, the end, away from the connecting piece, of the needle tube is in a round and blunt state, two transplanting through holes are symmetrically formed in the left side and the right side of the side wall of the needle tube, an auxiliary separation mechanism is arranged on the inner side of the needle tube, and the cross section of the needle tube is oval. Two groups of transplantation through holes symmetrically formed in the side wall of the needle tube and an auxiliary separation mechanism arranged in the needle tube jointly promote uniform distribution and effective transplantation of fat. The oval design and size distribution of the auxiliary separation beads are beneficial to better dispersing fat in the injection process, formation of fat masses is avoided, and therefore the transplantation effect is improved, the end, away from the connecting piece, of the needle tube is in a round and blunt state, the overall cross section of the needle tube is designed to be of an oval structure, and the injection efficiency is improved. By means of the design, damage to the tissue in the operation process can be reduced.

The invention discloses autologous fat transplantation equipment for increasing the survival rate of fat cells, and relates to the technical field of fat cell transplantation. Comprising an adipocyte transplantation processing mechanism, the adipocyte transplantation processing mechanism comprises a cell transplantation box, and a control panel is installed on the surface of the cell transplantation box; the device can conveniently extract transplanted and extracted fat cells, is beneficial to efficiently treating the transplanted fat cells, improves the fat cell transplantation and extraction effect, reduces the probability of errors caused by manual extraction of the fat cells by medical staff, can ensure that the fat cells are transplanted and extracted in a closed and sterile environment, and improves the survival rate of the fat cells. The fat cell transplantation and extraction device is simple in structure and convenient to use, the survival rate of fat cell transplantation and extraction is increased, medical articles for fat cell extraction can be stored, medical staff can conveniently operate in the fat cell transplantation and extraction work, the fat cell transplantation efficiency of the medical staff is improved, and convenience is provided for the staff.

The invention provides a method for assisting personalized lying silkworm operation reconstruction by evaluating the eye shape and the lying silkworm form. The method comprises the steps of collecting basic information of a beauty seeker, recording basic data information of eyes, and selecting a proper operation design scheme. The basic information of the beauty seeker comprises name, age, gender, eye surgery, trauma history and expected effect of the beauty seeker. The eye basic data information of the beauty seeker comprises an eyeball position, an upper and lower eyelid distance, an inner and outer canthus connecting line distance, a lying silkworm width, and an included angle formed by the inner and outer canthus connecting line and an eye crack horizontal line; according to the acquired basic information of the beauty seeker and the eye basic data information, the beauty seeker is assisted to select a proper lying silkworm reconstruction form. Scientific guidance can be provided for lying silkworm and periocular rejuvenation operations in the future, and a plastic surgeon can formulate a special autologous fat gel injection scheme according to facial features, skin states and personal requirements of a patient, so that the most natural and satisfactory effect is achieved.

The utility model provides an autologous adipose-derived stem cell kit which comprises a cuboid layered box body, and the layered box body is formed by sequentially connecting a sterile collection layer, an enzymolysis filtering layer, a centrifugal separation layer, a culture amplification layer and a low-temperature cryopreservation layer from top to bottom; the adjacent upper-layer structure and lower-layer structure are separately connected through a sealing buckle, and directional flowing of liquid is achieved through a one-way valve. An injector preloaded with anticoagulant and a blunt-end fat collection needle are arranged in the sterile collection layer, so that tissue damage is reduced. The autologous adipose-derived stem cell kit provided by the utility model can enable the existing kit to have the technical effect of multiple functions, can be used for extracting, amplifying and storing autologous adipose-derived stem cells, and has the technical effects that the whole process is closed, the operation is simple and convenient, and the cell viability can be improved.

The invention discloses a physical preparation method of purified adipose-derived stem cell gel with high shape plasticity and the adipose-derived stem cell gel. A physical method is adopted in the whole preparation process, exogenous pollution is avoided to a great extent, and the safety and effectiveness of a purified product are guaranteed. According to the method, fat gum prepared from autologous fat tissue is purified, grease contained in the fat gum and liquid components left when autologous fat is obtained are further removed, the inflammatory reaction after fat filling injection is relieved, and pSVF-gel has better appearance plasticity and can improve the filling effect after injection. According to the method, known SVF cell components and extracellular matrixes of SVF-gel are reserved, and the treatment effect of the further concentrated SVF cells on the wound surface is further improved compared with traditional fat and fat gum.

This invention relates to a medical device that generates a bioactive membrane using autologous fat and plasma. Belly fat is gently extracted using a syringe and passed through filters of different sizes to remove impurities. This purified fat is then mixed with platelet-rich plasma, which is made from the patient's blood. The mixture is placed in a special temperature-controlled incubator where it's shaken and exposed to red light. This apparatus facilitates the nano fat cells and iPRF to bind together, creating a thin, stretchy membrane. This membrane is then placed on the skin to cover injuries, scars, or create new skin. The fat membrane contains stem cells and growth factors, which promote healing and regeneration. It's a natural and safe way to improve skin health and appearance. This device has the potential to revolutionize plastic surgery and dermatology by offering new solutions for skin issues and enhancing recovery from procedures.

This invention discloses a medical fat collection device with separation and purification functions, relating to the field of fat extraction and collection technology. It includes an absorption gun and a collection component, with a connecting tube between the absorption gun and the collection component. A negative pressure pump connects the absorption gun and the collection component. When the negative pressure pump is activated, autologous fat is drawn in through the absorption gun. The fat mixture undergoes preliminary separation within the absorption gun, separating the fat from most of the blood. The pre-separated fat is then transported from the absorption gun to the collection component through the connecting tube. Once in the collection component, the fat undergoes further separation, purifying it further while separating any remaining blood. This achieves the separation and extraction of fat. By aspirating and processing the fat, it can be transplanted to other areas, avoiding rejection problems associated with fat transplantation and improving the quality of plastic surgery and trauma repair procedures.

The invention relates to a method for performing an autologous adipose tissue transplant in a patient. The method may include extracting a first amount of adipose tissue from the patient into a first syringe and extracting a second amount of adipose tissue from the patient into a second syringe. The first amount of adipose tissue can be decanted to isolate the fat from the first amount of adipose tissue. The second amount of adipose tissue can be transferred into a collection tube. A stromal vascular fraction can be isolated from the second amount of adipose tissue, the stromal vascular fraction being collected in a distal portion of the collection tube. The stromal vascular fraction can then be combined with the fat, and the fat and stromal vascular fraction can be deposited into the patient.