The utility model provides an autologous adipose-
derived stem cell kit which comprises a cuboid layered box body, and the layered box body is formed by sequentially connecting a sterile collection layer, an enzymolysis filtering layer, a
centrifugal separation layer, a culture amplification layer and a low-temperature
cryopreservation layer from top to bottom; the adjacent upper-layer structure and lower-layer structure are separately connected through a sealing buckle, and directional flowing of liquid is achieved through a one-way valve. An
injector preloaded with
anticoagulant and a blunt-end fat collection needle are arranged in the sterile collection layer, so that
tissue damage is reduced. The autologous adipose-
derived stem cell kit provided by the utility model can enable the existing kit to have the technical effect of multiple functions, can be used for extracting, amplifying and storing autologous adipose-
derived stem cells, and has the technical effects that the whole process is closed, the operation is simple and convenient, and the
cell viability can be improved.