140 results about "Autologous fat" patented technology

A novel human breast implant and method for using the same comprising a bioabsorbable implant into which native, autologous vascularized tissue and autologous fat is placed and propagated within a patient's chest as a breast implant.

A method is disclosed for preparing a soft tissue site, and augmenting the soft tissue site, such as the breast(s), scar, depression, or other defect, of a subject through use of devices that exert a distractive force on the breast(s) and grafting of autologous fat tissue such as domes with sealing rims for surrounding each of the soft tissue site and a regulated pump. The method for preparing the soft tissue site, and enhancing fat graft results, entails application of the distracting force to the targeted soft tissue site at least intermittently for some period of time and preferably several weeks prior to the graft procedure. A related aspect of the invention includes following the preparation steps by transfer of fat from other areas of the subject to the subject's soft tissue site, and then reapplication of the distractive force to the soft tissue site that received the autologous fat graft. Alternatively, fat from genetically related sources may be used, and the fat may be further processed prior to injection. Substantial soft tissue augmentation, high rates of graft survival and negligible graft necrosis (data demonstrating 80% survival and only 20% necrosis is presented) or calcification result from the practice of these methods.

A novel human breast implant and method for using the same comprising a bioabsorbable implant into which native, autologous vascularized tissue and autologous fat is placed and propagated within a patient's chest as a breast implant.

One purpose of the invention is to provide a method and a formula, which is characterized in that mesenchymal stem cells from an autologous fat cell tissue are proliferated by in-vitro culture, then is mixed into a support material and finally is transplanted into the body of a patient; the fat mesenchymal cell in a transplant is differentiated to derma fibroblast under the action of the local microenvironment, the newly generated derma fibroblast can further activate secretion of cell collagenstroma at an injection part so as to carry out reproductive repair on coloboma and aging of human skin tissue. The application scope of the invention includes: subcutaneous padding for face lift, subcutaneous padding cosmetology, subcutaneous padding for repairing depressed deletion or impairment and the like. Seed cells utilized in the invention are prepared by separating and purifying the fat tissues of the patients, so that ethical disputes and immunological rejection reaction are avoided. The fat tissues can be obtained by the instrument suction method; and the operation of the method is simple, patients suffer from minor damages and pains.

System and methods for collecting and preparing fat for autologous fat tissue transplantation. The system includes syringes, filtering and transferring assemblies coupled with the syringes, a centrifuge and a centrifuge insert releasably coupled with the centrifuge. Substances, including oil, fat, and denser substances, such as tumescent fluid, connective tissue, hormones and / or other non-fat substances, are harvested through a cannula into a syringe. Syringes are placed in the centrifuge insert, and subject to centrifugation. Centrifugation stratifies the substances into a top oil stratum, a middle fat stratum, and a bottom denser substance stratum. Denser substances are filtered out of the syringes during or after centrifugation. Oil is removed through an aspiration port in the plunger head of the syringe plunger. Fat is transferred from the first syringe into one or more smaller syringes through an adapter in the assembly coupled with the first syringe and the smaller syringe.

The invention provides an autologous fat collecting, cleaning and transplanting device. Extraction and adsorption equipment is composed of a double-layer injector, a three-way liposuction needle assembly, an adsorption bag, a first connection hose and a negative pressure connecting pipe, an openings are formed in the surface of an inner needle cylinder body of the double-layer injector, and the three-way liposuction needle assembly comprises a first needle head and a first three-way plastic pipe. A liquid outlet end of a needle cylinder is sleeved with the first three-way plastic pipe, one end of the first connection hose is inserted into the first three-way plastic pipe, and the other end of the first connection hose is inserted into a liquid inlet pipe of the adsorption bag. One end of the negative pressure connecting pipe is inserted into a negative-pressure liposuction machine, and the other end of the negative pressure connecting pipe is inserted into a liquid outlet pipe on the left lower part of the needle cylinder. Filtration and injection equipment is composed of an injector, a three-way fat injecting needle assembly, a second connection hose and a filter, and the three-way fat injecting needle assembly comprises a second needle head and a second three-way plastic pipe. The liquid outlet end of a needle cylinder of the injector is sleeved with the lower end of the second three-way plastic pipe, one end of the second connection hose is inserted into the right end of the second three-way plastic pipe, and the other end of the second connection hose is inserted into a liquid outlet pipe of the filter. By means of the autologous fat collecting, cleaning and transplanting device, convenience is brought to liposuction, cleaning and injection.

The invention discloses an autologous fat filler and a preparation method thereof, wherein the autologous fat filler is constructed by simulating a tissue engineering method. The autologous fat filler comprises the following ingredients of a cytoskeleton, a cellular constituent, a growth factor and a scar resistant factor at the volume ratio of (109-115):(15-30):4:0.5. The effective ingredients are combined, the cytoskeleton part provides a skeleton made of a plant cell ingredient, an injectable gel is favorable for the adhesion of a cell, a washing fat occupies most of a filling volume, a stem cell and an SVF (Stromal Vascular Fraction) component are differentiated into an adipose cell and a vascular endothelial cell, and various excreted factors are good for maintaining a transplanting microenvironment and generating a blood vessel. According to the provided autologous fat filler and the preparation method thereof, the stability of the transplanting fat is effectively improved, the absorption of the transplanted fat is reduced, the untoward effect of fibrosis of a transplant is reduced, and the development of an autologous fat face filling plasty is promoted.

The invention discloses exosome freeze-drying powder, a preparation method of the exosome freeze-drying powder and a preparation comprising the exosome freeze-drying powder. The preparation method of the exosome freeze-drying powder comprises the following steps of: preparing platelet factor-rich plasma: collecting autologous blood for centrifugation, preparing exosome: collecting autologous fat to prepare fat mesenchymal stem cells, and culturing the mesenchymal stem cells, preparing a freeze-drying liquid: mixing the platelet factor-rich plasma and the exosome and adding trehalose for mixing, and performing freeze-drying: performing freeze-drying on the freeze-drying solution. The preparation comprises the exosome freeze-drying powder, hyaluronic acid and saline. The preparation can effectively solve the problems that the existing cosmetic preparation has a poor filling effect, short maintenance time and high immunological rejection response.

Methods and kits for producing cellular fractions enriched in adipose derived stem cells. Methods are provided where adipose tissue obtained from liposuction is enzymatically treated using a solution containing collagenase and divalent cations prior to the application of traditional methods of stromal-vascular fraction isolation. The enzymatic solutions may contain collagenase types I and II to a final concentration of about 0.001 mg / ml to 0.010 mg / ml. The divalent cations may be present as calcium, magnesium, and zinc chloride. The final concentration of calcium, magnesium, and zinc may range from about 0.001 to 0.1 micromolar; about 0.005 to 0.5 micromolar; and about 0.0015 to 0.15 micromolar, respectively. The enzymatic solutions may be generated using a kit where the collagenase and divalent components are held in separate containers until just prior to use. The cellular fractions isolated in this manner may be used in autologous fat grafts in therapeutic applications.

The invention discloses a method for acquiring EPCs (endothelial progenitor cells) from autologous fat tissue of human beings or animals. The method comprises steps of efficient separation and enrichment of the fat EPCs, a culture dish is not required to be enveloped in advance in the separation process, multiplication culture is performed in vitro, the purity of the obtained EPCs is high, the dryness and the proliferation capability of the cells are high, and the EPCs have the typical EPC form and cell phenotype as well as low-density lipoprotein uptake and blood vessel formation capability.