142 results about "Autologous fat" patented technology

A method is disclosed for preparing a soft tissue site, and augmenting the soft tissue site, such as the breast(s) of a subject through use of devices that exert a distractive force on the breast(s) and grafting of autologous fat tissue such as domes with sealing rims for surrounding each of the breasts and a regulated pump. The method for preparing the soft tissue site, and enhancing fat graft results, entails application of the distracting force to the targeted soft tissue site for several weeks prior to the graft procedure. A related aspect of the invention includes following the preparation steps by transfer of fat from other areas of the subject to the subject's breast(s), and then reapplication of the distractive force to the breast(s) that received the autologous fat graft. Alternatively, fat from genetically related sources may be used, and the fat may be further processed prior to injection. Substantial breast augmentation, high rates of graft survival and negligible graft necrosis (data demonstrating 80% survival and only 20% necrosis is presented) or calcification result from the practice of these methods.

A novel human breast implant and method for using the same comprising a bioabsorbable implant into which native, autologous vascularized tissue and autologous fat is placed and propagated within a patient's chest as a breast implant.

A novel human breast implant and method for using the same comprising a bioabsorbable implant into which native, autologous vascularized tissue and autologous fat is placed and propagated within a patient's chest as a breast implant.

A novel human breast implant and method for using the same comprising a bioabsorbable implant into which native, autologous vascularized tissue and autologous fat is placed and propagated within a patient's chest as a breast implant.

One purpose of the invention is to provide a method and a formula, which is characterized in that mesenchymal stem cells from an autologous fat cell tissue are proliferated by in-vitro culture, then is mixed into a support material and finally is transplanted into the body of a patient; the fat mesenchymal cell in a transplant is differentiated to derma fibroblast under the action of the local microenvironment, the newly generated derma fibroblast can further activate secretion of cell collagenstroma at an injection part so as to carry out reproductive repair on coloboma and aging of human skin tissue. The application scope of the invention includes: subcutaneous padding for face lift, subcutaneous padding cosmetology, subcutaneous padding for repairing depressed deletion or impairment and the like. Seed cells utilized in the invention are prepared by separating and purifying the fat tissues of the patients, so that ethical disputes and immunological rejection reaction are avoided. The fat tissues can be obtained by the instrument suction method; and the operation of the method is simple, patients suffer from minor damages and pains.

Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called “spun fat”. That “spun fat” can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the “spun fat”. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).

The invention provides a method for preparing adipose derived stem cell bioactive peptide freeze-dried powder. The preparation method includes the following steps that S1, autologous fat tissue is extracted; S2, primary-generation fat stem cells are prepared from the autologous fat tissue; S3, fifth-generation fat stem cells are prepared from the primary-generation fat stem cells, and cell culture supernatant fluid is obtained; S4, the cell culture supernatant fluid is centrifuged, a supernatant solution is collected, and the supernatant solution is filtered; S5, after ultrafiltration and concentration are carried out, a freeze-drying protective additive is added for freeze drying, and the adipose derived stem cell bioactive peptide freeze-dried powder is obtained. Through a series of operating steps, an active factor mixture in adipose derived stem cell culture supernatant is preserved in the form of freeze-dried powder, obtained factors are completely derived from autografts, use is safe and reliable, and the possibility of allergy and infection of exogenous pathogens in the using process is eliminated.

The invention provides an autologous fat collecting, cleaning and transplanting device. Extraction and adsorption equipment is composed of a double-layer injector, a three-way liposuction needle assembly, an adsorption bag, a first connection hose and a negative pressure connecting pipe, an openings are formed in the surface of an inner needle cylinder body of the double-layer injector, and the three-way liposuction needle assembly comprises a first needle head and a first three-way plastic pipe. A liquid outlet end of a needle cylinder is sleeved with the first three-way plastic pipe, one end of the first connection hose is inserted into the first three-way plastic pipe, and the other end of the first connection hose is inserted into a liquid inlet pipe of the adsorption bag. One end of the negative pressure connecting pipe is inserted into a negative-pressure liposuction machine, and the other end of the negative pressure connecting pipe is inserted into a liquid outlet pipe on the left lower part of the needle cylinder. Filtration and injection equipment is composed of an injector, a three-way fat injecting needle assembly, a second connection hose and a filter, and the three-way fat injecting needle assembly comprises a second needle head and a second three-way plastic pipe. The liquid outlet end of a needle cylinder of the injector is sleeved with the lower end of the second three-way plastic pipe, one end of the second connection hose is inserted into the right end of the second three-way plastic pipe, and the other end of the second connection hose is inserted into a liquid outlet pipe of the filter. By means of the autologous fat collecting, cleaning and transplanting device, convenience is brought to liposuction, cleaning and injection.

The invention belongs to the field of biomedicine, and particularly discloses a preparation method of an adipose tissue breast augmentation material enriched with autologous stem cells. In the present invention, only 5ml of tissue is extracted from the patient, and a large number of adipose stem cells are obtained through separation, purification, and in vitro culture and expansion, and finally added to the newly prepared SVF components, mixed with adipose tissue for breast augmentation transplantation. The invention can reduce the amount of adipose tissue used for preparing SVF during fat transplantation, so as to make up for the disadvantage that a large number of women cannot obtain a large amount of adipose tissue to prepare SVF due to low body mass index and cannot perform autologous fat breast augmentation.

The present invention discloses one kind of cartilage graft, which includes pharmaceutically acceptable biodegradable material and fat stem cell and is in the shape matching the cartilage defect part to be repaired. The tissue engineering cartilage constructed with the autologous fat stem cell may be used in treating cartilage impairment effectively. The present invention also provides the preparation process and use of the tissue engineering cartilage.

The invention discloses an autologous fat filler and a preparation method thereof, wherein the autologous fat filler is constructed by simulating a tissue engineering method. The autologous fat filler comprises the following ingredients of a cytoskeleton, a cellular constituent, a growth factor and a scar resistant factor at the volume ratio of (109-115):(15-30):4:0.5. The effective ingredients are combined, the cytoskeleton part provides a skeleton made of a plant cell ingredient, an injectable gel is favorable for the adhesion of a cell, a washing fat occupies most of a filling volume, a stem cell and an SVF (Stromal Vascular Fraction) component are differentiated into an adipose cell and a vascular endothelial cell, and various excreted factors are good for maintaining a transplanting microenvironment and generating a blood vessel. According to the provided autologous fat filler and the preparation method thereof, the stability of the transplanting fat is effectively improved, the absorption of the transplanted fat is reduced, the untoward effect of fibrosis of a transplant is reduced, and the development of an autologous fat face filling plasty is promoted.

The invention discloses a self-purified fat quantitative transplanting device and relates to the field of autologous fat transplantation plastic surgery instruments, which is in particular suitable for a little or quantitative fat transplantation during autologous fat transplantation plastic surgery and acts on separation of fat and liquid outside the fat and implantation of purified fat. According to the self-purified fat quantitative transplanting device disclosed by the invention, injector cylinders of different volumes are respectively and uniquely matched, and an injector cylinder piston head replaces an existing injector cylinder piston push rod so that the original changeless push rod is changed to be a channel with a pipe, the channel is provided with a filter screen with the certain aperture, a piston, a negative-pressure regulating hole and a negative-pressure pump connection pipe joint; a product is directly inserted into the injector cylinder, a negative-pressure pump provides negative pressure, a fat extracting needle is directly inserted into a fat-extracted part of a human body, fat enters the injector cylinder after leaving from the human body, the fat is left in the injector cylinder after a fat and water mixture in the injector cylinder passes through the filter screen under negative pressure, other liquids are separated from the fat after passing through a negative-pressure pipe connected with the negative-pressure pump connection pipe joint, and the purified fat is used for being directly transplanted to a required position; and therefore, purposes of saving time and labor and realizing safety and effectiveness are achieved.

The present invention belongs to the technical field of medical devices and particularly discloses an autologous fat living cell extraction device. The autologous fat living cell extraction device comprises a syringe cylinder, a piston is slidably arranged in the syringe cylinder, a bottom part of the piston is fixedly provided with a sliding rod, a bottom end of the sliding rod extends outside the syringe cylinder, the sliding rod is fixedly provided with gear racks, one side of the syringe cylinder is fixedly provided with a first fixing block and a second fixing block, the first fixing block and the second fixing block are fixedly provided with a same support rod, the support rod is slidably provided with a mounting rod, the support rod is slidably sleeved with a first spring, a top endof the first spring is fixedly connected with the mounting rod, and a bottom end of the first spring is fixedly connected with the second fixing block. The autologous fat living cell extraction device is simple in operations and strong in practicability, can effectively prevent liposuction needles from invading patient's body to cause harms to the patient due to slippage of medical staff's hands,eliminates safety hazard and reduces medical risks.

The invention discloses exosome freeze-drying powder, a preparation method of the exosome freeze-drying powder and a preparation comprising the exosome freeze-drying powder. The preparation method of the exosome freeze-drying powder comprises the following steps of: preparing platelet factor-rich plasma: collecting autologous blood for centrifugation, preparing exosome: collecting autologous fat to prepare fat mesenchymal stem cells, and culturing the mesenchymal stem cells, preparing a freeze-drying liquid: mixing the platelet factor-rich plasma and the exosome and adding trehalose for mixing, and performing freeze-drying: performing freeze-drying on the freeze-drying solution. The preparation comprises the exosome freeze-drying powder, hyaluronic acid and saline. The preparation can effectively solve the problems that the existing cosmetic preparation has a poor filling effect, short maintenance time and high immunological rejection response.

The invention belongs to the technical field of construction of artificial organs by a tissue engineering method in biomedical engineering and relates to a tissue engineered breast transplant and a constructing method thereof. The tissue engineered breast transplant of the invention takes injectable material I type collagen gel as a carrier bracket and takes adipose-derived stem cells as seed cells; the seed cells are attached to the carrier bracket to form an adipose-derived stem cell / I type collagen gel compound; the final concentration of collagen is 2 mg / ml; and the cell density is 2*10<7> / ml. The tissue engineered breast transplant of the invention consists of injectable material-loaded autologous adipose-derived stem cells and is simple and convenient to prepare. Experiments prove that a new adipose tissue can be produced and the tissue engineered breast transplant is finally converted into autologous fat without an immunological rejection problem, so a new solution is provided for the filling and reconstruction of a breast.

The invention relates to the application field of adipose-derived stem cells, and in particular to a method for improving fat filling effect of face and other parts by using adipose-derived stem cells(ADSCs). The method mainly comprises extraction of fat and extraction and concentration of ADSCs and adipose cells, wherein the extraction of fat can be performed by selecting upper arms, abdomen, inner sides and outer sides of thighs as supply areas and marking according to subjective requirements and self conditions of experimenters. By controlling in-vitro treatment time of the ADSCs and combining with ASCs assisted fat transplantation technology, the method improves survival rate of transplanted fat cells on face, effectively improves user satisfaction after facial filling, and reduces morbidity of fat transplantation complications and sequelae.

The method includes novel applications for liposuction, lipectomy, liposculpting, and ultrasonic fat harvesting and emulsification devices, especially those devices that provide in situ visualization. The application includes immediately transferring harvested autologous fat from the liposuction, lipectomy, liposculpting, or ultrasonic device to an injector portion on the fat harvesting and emulsifying device or to a separate injector unit that injects it into muscle to provide a higher value, more desirable, use for and positioning of the fat in the form of muscle volume expansion.

A method and a device for autografting of fat microparticles containing stem cells of various types, injected into scars, or stretch marks, and / or other cutaneous injuries, at epidermis-dermis level is presented. Fat is taken from the patient in the periumbilical area, where large quantities of mesenchymal stem cells exist. After reduction in size by means of a thin grid emulsifier filter the fat microparticles are loaded into a tank of a multi-needle gun, such as used for tattoos. The multi-needle tattoo gun injects the fractionated fat microparticles into the epidermis-dermis of scars, and / or stretch marks and / or other skin defects or even internal injuries. The injected fat particles trigger tissue regeneration, deleting scars, stretch marks, wrinkles and / or other defects or skin damages in 3-4 weeks. This method for regeneration of tissues can be extended to all surgeries, including vital organs of the human body; always by means of tattooing the target organ with autologous fat microparticles containing stem cells, wherein access is through open, endoscopic, and / or laparoscopic surgery.

Methods and kits for producing cellular fractions enriched in adipose derived stem cells. Methods are provided where adipose tissue obtained from liposuction is enzymatically treated using a solution containing collagenase and divalent cations prior to the application of traditional methods of stromal-vascular fraction isolation. The enzymatic solutions may contain collagenase types I and II to a final concentration of about 0.001 mg / ml to 0.010 mg / ml. The divalent cations may be present as calcium, magnesium, and zinc chloride. The final concentration of calcium, magnesium, and zinc may range from about 0.001 to 0.1 micromolar; about 0.005 to 0.5 micromolar; and about 0.0015 to 0.15 micromolar, respectively. The enzymatic solutions may be generated using a kit where the collagenase and divalent components are held in separate containers until just prior to use. The cellular fractions isolated in this manner may be used in autologous fat grafts in therapeutic applications.

The invention discloses an autologous fat active cell filtration device for cosmetic surgery. The autologous fat active cell filtration device comprises a cell collection box of which the top part isopened, wherein a filter plate is arranged on the top of the cell collection box; a through groove which penetrates through the filter plate in the left -right direction is formed in the filter plate;the filter plate is also provided with a filter hole which penetrates through the filter plate in the thickness direction and penetrates through the through groove; a driving wheel and a driven wheelare respectively arranged on the left side and the right side of the cell collection box; a conveyor belt is wound between the driving wheel and the driven wheel and penetrates through the through groove; the conveyor belt comprises a plurality of elastic filter screens having mesh holes of different sizes; the elastic filter screens are connected together through a rubber belt; the width of eachof the elastic filter screens is consistent with the width of the through groove; the driving wheel is driven through a driving structure to be rotated; a cleaning liquid spray nozzle is formed abovethe driven wheel; and a cleaning liquid recycling box is arranged below the cleaning spray nozzle. Through the filtration device of the structure, various impurities in the active cells can be effectively removed, and the filtration efficiency and effect are effectively improved.

The invention discloses a method for acquiring EPCs (endothelial progenitor cells) from autologous fat tissue of human beings or animals. The method comprises steps of efficient separation and enrichment of the fat EPCs, a culture dish is not required to be enveloped in advance in the separation process, multiplication culture is performed in vitro, the purity of the obtained EPCs is high, the dryness and the proliferation capability of the cells are high, and the EPCs have the typical EPC form and cell phenotype as well as low-density lipoprotein uptake and blood vessel formation capability.

The invention relates to an application of an interstitial vascular cell and a mesenchymal progenitor cell in the prevention or treatment of rheumatoid arthritis. A medicinal composition containing the interstitial vascular cell and the mesenchymal progenitor cell from autologous fat is applied to a necessary object in order to substantially prevent or treat rheumatoid arthritis. The mesenchymal progenitor cell has a very high cytokine secretion ability, and can restore in-vivo body damages; and the interstitial vascular cell and the mesenchymal progenitor cell have chondroblasting and ossification ability. The invention also provides the medicinal composition containing the interstitial vascular cell and the mesenchymal progenitor cell, and a method for preventing and treating the rheumatoid arthritis.

The invention discloses an autologous fat multi-component full-automatic separation, preparation and extraction device and method. The autologous fat multi-component full-automatic separation, preparation and extraction device comprises a dry-wet separation unit, a fat disruption and cell extraction unit, an exosome preparation unit and a centrifugal extraction unit. According to the full-automatic separation, preparation and extraction method for multiple components of autologous fat, connective tissues, fat and mixed liquid are obtained by the dry-wet separation unit, and filling particles, fat particles, adipose stem cells or vascular matrix components are obtained by the fat crushing and cell extraction unit; an exosome concentrated solution is obtained from the mixed liquid or an exosome culture solution obtained from adipose-derived stem cells by the exosome preparation unit, and the exosome concentrated solution is centrifugally separated by the centrifugal extraction unit and then is sucked and sampled. The method is complete in prepared and extracted component variety, high in extraction efficiency, high in automation degree, capable of efficiently utilizing autologous fat, high in extracted component activity, low in pollution, and suitable for clinical application or commercial mass production.

The invention discloses a liposuction device for autologous fat transplantation of the human body and a usage method thereof and belongs to the field of medical surgical operation devices. A fat quantification device and a fat storage device are arranged in an incubator, and a temperature sensor I and a temperature sensor II are arranged in a liposuction end head and the incubator respectively. A pressure pump switch for starting a pressure pump or not through controlling, a pressure display screen and a pressure regulating knob are all arranged on the pressure pump. A thin water body impact tube is arranged on a delivery pipeline between the fat quantification device and the fat storage device. A primary filter layer and a secondary filter layer are sequentially arranged in the fat storage device from top to bottom. A more suitable storage environment can be provided for fat cells, and keeping of the activity of the fat cells is promoted. Misoperations produced by excessive and easily mistaken switching and the situation that normal operation cannot be performed in time can be prevented. The keeping of the survival rate of the fat cells is promoted. The accuracy of fat quantification can be ensured, and collection and reutilization of the fat cells and residual materials are promoted.

The invention relates to an autologous adipose cell cryopreservation solution and a cryopreservation method of substances such as adipose tissue-derived stromal cells. Every 100 mL of the autologous adipose cell cryopreservation solution comprises the following content components: 20 to 38 mL of mesenchymal stem cell culture supernatant, 3 to 5 g of polyvinylpyrrolidone, 3 to 5 mL of glycerinum, 1.5 to 2 g of hydroxyapatite nanoparticles, 1.5 to 2 g of trehalose, 0.5 to 1 g of polyvinyl alcohol, 0.01 to 0.1 g of human serum albumin, 0.05 to 0.3 g of adenosine triphosphate, 0.5 to 1.5 mg of all-trans retinoic acid, 0.3 to 0.8 g of vitamin C, 0.2 to 0.6 g of vitamin E and 0.2 to 0.6 g of lipoic acid. Compared with the prior art, ice crystal formation can be effectively reduced, and cell damage caused by uneven temperature conduction and sharp ice crystal formation is prevented.

The invention provides a multifunctional autologous fat transplantation device maintaining fat cell activity for a short period. The device comprises a needle guard, a syringe needle, and an injector body. The injector body comprises a needle cylinder and a plunger. The front end of the needle cylinder is provided with a needle tubing connected with the syringe needle. The needle cylinder is provided with scales. The needle cylinder is internally provided with a filter screen fixed on the inner wall of the needle cylinder. The needle cylinder is provided with a cell culture fluid storage tank. The cell culture fluid storage tank is internally provided with a fat cell culture fluid. The cell culture fluid storage tank and the needle cylinder are connected through a first solenoid valve. The invention provides the multifunctional device which integrates fat cell extraction, analysis, and injection, and the device provides stable environment which maintains fat cell activity for a short period. One-time extraction of fat cells and low-intensity and high-frequency injection can be performed, and steps of repeated extraction are reduced, and effect of low-intensity and high-frequency injection is improved.

The invention discloses mesenchymal stem cells and exosomes of the mesenchymal stem cell and application of the mesenchymal stem cells in treating anal fistula, and further discloses a method for treating anal fistula by using an autologous-fat-derived mesenchymal stem cell mixed preparation. The stem cell preparation includes autologous-adipose-derived mesenchymal stem cells (ADSCs) and exosomes(EXO-ADSCs) of the ADSCs, autologous platelet-rich plasma (PRP) and synthetic bacteriostatic peptides (AMP). The stem cells have immunoregulatory functions, the exosomes create a micro-environment which is conducive to recovery, meanwhile, the stem cells are stimulated to release various growth factors, and wound healing is promoted. PROP gel provides support and nutrients and works synergistically with stem cells, the survival rate after stem cell transplantation is improved, and the stem cells can better play a role. The synthetic peptides are single in components and controllable and reducethe incidence of infection. The combined use greatly improves the cure rate of anal fistula.

The invention relates to application of stromal vascular fraction cells and mesenchymal progenitor cells to the prevention or treatment of osteoarthritis. Specifically, remarkable prevention or treatment effects on osteoarthritis are achieved by applying a medicinal composition containing the stromal vascular fraction cells and the mesenchymal progenitor cells from autologous fat to an object with requirements. The mesenchymal progenitor cells have high cytokine secretion capability and the effect of repairing damage to organisms in a body. The stromal vascular fraction cells and the mesenchymal progenitor cells have chondrogenic differentiation and bone formation capability. The invention also provides the medicinal composition containing the stromal vascular fraction cells and the mesenchymal progenitor cells and a method for preventing and treating osteoarthritis.