13 results about "Sanguinarine" patented technology

The invention discloses an application of sanguinarine chloride (SGC) in preparation of a medicine for treating a PRCC-TFE3 rearranged renal cell carcinoma (rRCC), and belongs to the technical field of biological medicine, in particular to an application of sanguinarine chloride (SGC) in preparation of a medicine for treating a PRCC-TFE3 rearranged renal cell carcinoma (rRCC). According to the application, it is found for the first time that SGC has a specific killing effect on PRCC-TFE3 rRCC cells and has extremely low toxicity on normal kidney cells; the SGC has multiple anti-tumor action mechanisms: on one hand, the SGC is directly combined with and antagonizes a VEGFR2 receptor on a cell membrane to block autocrine signal transduction of VEGFB; on the other hand, the SGC inhibits transcription of VEGFB (vascular endothelial growth factor B) through ROS / p-STAT3 axis epigenetics; besides, the SGC can inhibit the production of lactic acid and the acylation of H3K18, and down-regulate the secretion of chemotactic factors, so that the infiltration of polymorphic myeloid-derived suppressor cells (PMN-MDSCs) in a tumor microenvironment is remarkably inhibited; in an immune sound mouse model, the SGC shows an excellent in-vivo anti-tumor effect, and a brand new targeting and immunoregulation double-effect candidate drug is provided for clinically treating the PRCC-TFE3 rRCC lacking an effective standard therapy.

The invention discloses application of sanguinarine in preparation of a medicine for preventing and treating fatty liver or obesity, and belongs to the technical field of medicines. The invention is implemented in vitro and in vivo, and aims to solve the problem of lack of low-toxicity natural small molecule drugs for effectively preventing and treating fatty liver or obesity at present. The embodiment of the invention proves that sanguinarine can obviously reduce high-fat diet induced mouse fat deposition, reduce in-vivo and in-vitro liver TG content and lipid droplet accumulation, enhance glucose tolerance of the high-fat diet induced mouse and improve insulin resistance of the high-fat diet induced mouse. As a natural antioxidant, sanguinarine has an obvious relieving effect on energy metabolism disorder and oxidative stress induced by high fat. As a low-toxicity natural small molecule, sanguinarine can improve glucose and lipid metabolism disorder induced by high fat, is low in price, and has an application prospect in preparation of medicines for preventing and treating fatty liver or obesity.

The invention relates to the technical field of biology, in particular to novel application of sanguinarine (SAN), a method for inducing human amniotic mesenchymal stem cells (hAMSCs) to be differentiated into osteoblasts through COX2 protein and an osteogenic induction culture medium for the inducing method, and the method can be applied to the fields of bone disease treatment drug research and development and clinical transformation. It is proved for the first time that SAN mediates hAMSCs osteogenic differentiation through COX2 protein, SAN can significantly improve expression of COX2 gene (PTGS2) in hAMSCs, the osteogenic effect of SAN can be reversed by adding a COX2 inhibitor (such as celecoxib), and direct experimental evidence is provided for'COX2 serving as a small molecule drug to promote osteogenic differentiation '.

The present application relates to the application of sanguinarine in the preparation of drugs for preventing and treating rare clinical fungal infection diseases. Apiotrichum mycotoxinivorans has been confirmed to have the ability to infect humans, and current research confirms that the fungus is at least associated with postoperative pulmonary infection and blood infection diseases. Since the above fungus is still a rare clinical pathogenic bacterium, there is no clear and feasible treatment drug at present. Based on the advantages of low toxicity and side effects of natural drugs and mild efficacy, the present application screens natural drugs with bacteriostatic effect, and provides the application of sanguinarine as a fungus inhibitor, which has good clinical application prospect.

The invention discloses a sanguinarine sterilization composition containing a macleaya cordata extract and application of the sanguinarine sterilization composition. The sanguinarine sterilization composition mainly comprises sanguinarine and pydiflumetofen. The two components do not have cross resistance, the two medicaments have obvious synergistic effect after being combined according to a certain ratio, and a common agricultural bactericide dosage form is prepared by adding a proper amount of agricultural bactericide auxiliaries and has higher control effect on fungal diseases of crops. Through combined use of the formula, the dosage can be reduced, the drug resistance of germs to a single agent can be delayed, ecological damage and environmental pollution are effectively reduced, and the yield and quality of crops can be improved; the composition can efficiently prevent and control crop sickle root rot, increases a disease prevention spectrum, and can prevent common crop diseases such as anthracnose, leaf spot, early blight, brown blotch and gray mold at the same time.

The invention belongs to the technical field of plant extraction, and discloses influence of different extraction processes on yield of macleaya cordata extract and application of the macleaya cordata extract in grass carp feed. The invention discloses a macleaya cordata extract preparation method, which comprises: mixing macleaya cordata and an acidic ethanol solution, carrying out reflux extraction, collecting the extraction liquid, and concentrating to obtain the macleaya cordata extract. According to the method disclosed by the invention, the process conditions mainly involved in the extraction process of the macleaya cordata, such as the concentration of ethanol, the material-liquid ratio of acidic ethanol to macleaya cordata powder, the reflux extraction time and the temperature condition during concentration, are investigated. The extraction process of the macleaya cordata is moderately optimized by detecting the content of sanguinarine in the extract, so that the optimal extraction process of the macleaya cordata extract is obtained, and the extraction efficiency of alkaloid (sanguinarine) in the macleaya cordata can be effectively improved by the process.

The invention discloses a macleaya cordata recovery prescription medicine for preventing and treating fish nocardia disease as well as a preparation method and application of the macleaya cordata recovery prescription medicine. The compound medicine consists of 10 to 30 percent of macleaya cordata alkaloid hydrochloride compound, 5 to 15 percent of chitosan quaternary ammonium salt, 10 to 20 percent of mannan oligosaccharide and a medicinal carrier. The preparation method comprises the following steps: carrying out freezing and heat shock pretreatment on a macleaya cordata raw material, and carrying out ultrasonic-flash synergistic extraction by adopting acidified ethanol; performing concentration, macroporous resin purification and high-speed counter-current chromatography separation on the extracting solution to obtain a high-purity macleaya cordata alkaloid hydrochloride compound; and finally, mixing the compound with chitosan quaternary ammonium salt and a carrier, granulating, and carrying out enteric coating. According to the method, active alkaloids such as sanguinarine and chelerythrine in macleaya cordata can be efficiently extracted and refined. According to the compound medicine, through scientific compatibility, for example, macleaya cordata alkaloid is antibacterial, chitosan quaternary ammonium salt and mannan oligosaccharide are used for enhancing immunity and targeting, the bioavailability is improved by utilizing a modern preparation technology, and the compound medicine has a good effect of preventing and treating fish nocardia disease.

The present application relates to the field of medicine synthesis, and discloses a synthetic method of a sanguinarine intermediate compound bromide and application thereof. First, compared with the prior art, the synthetic method of the sanguinarine intermediate compound bromide can avoid using a difficult-to-prepare starting material, has short steps, mild reaction conditions, is easy to operate, has low cost, is environmentally friendly, has high yield and high product purity. Secondly, compared with the prior art, the sanguinarine hemisulfate synthesized by using the above bromide as a raw material can greatly reduce the amount of noble metal palladium catalyst, avoid using silver carbonate as an alkali (acid binding agent), and through conventional post-treatment operations such as extraction, beating and concentration, high-purity sanguinarine hemisulfate can be obtained without column chromatography separation.

The invention relates to a pesticide insecticidal composition and a preparation method and application thereof, and relates to the field of pesticides, the effective components comprise chlorantraniliprole and sanguinarine sulfate, and the weight ratio of chlorantraniliprole to sanguinarine sulfate is 99: 1-1: 99. According to the invention, chlorantraniliprole and sanguinarine sulfate are compounded together, so that pests can be killed to the greatest extent, and meanwhile, crops are stimulated to repair damage, so that the purposes of repairing after pests and rejuvenating are achieved. Subsequent growth of crops is guaranteed, and social value and economic value of stabilizing and increasing yield and improving quality are achieved.

The invention belongs to the technical field of biological medicines, and discloses a toxoplasma gondii biomarker and an application thereof in order to solve the technical problem of relatively less research on TgAP2IX-5 regulation of toxoplasma gondii at present, and particularly, a G-quadruplex nucleic acid sequence is formed in a TgAP2IX-5 gene promoter region, and the TgAP2IX-5 gene promoter region is constructed to obtain the toxoplasma gondii biomarker. And screening of toxoplasma gondii-resistant drugs is carried out by taking the synthesized G-quadruplex as a target spot. The screened small molecular ligands sanguinarine and TMPyP4 have obvious effects of inhibiting transcription of the TgAP2IX-5 gene and proliferation of toxoplasma gondii, and the action effect is superior to that of a clinically common therapeutic drug sulfadiazine; and a good treatment effect can be achieved at a relatively low concentration.

The invention discloses application of sanguinarine and simplified derivatives thereof in prevention and treatment of plant pathogenic bacteria, and relates to the technical field of medicinal chemistry. The sanguinarine and the simplified derivative thereof have a good inhibition effect on plant pathogenic bacteria such as rice bacterial blight pathogenic bacteria, citrus ulcer pathogenic bacteria and pseudomonas solanacearum pathogenic bacteria, the compound involved in the invention is low in preparation difficulty, the raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel antibacterial agent.

The application provides a preparation method of a natural product 6-HHS, which comprises the following steps: reacting a compound 1, sanguinare, with vinylmagnesium bromide to prepare a compound 2; and reacting the compound 2 with borane tetrahydrofuran complex to prepare 6-HHS. The application uses sanguinare as a raw material to prepare the natural product 6-HHS, has a short synthesis route, high synthesis efficiency, a yield of each step is more than 85%, the comprehensive yield of the natural product 6-HHS can reach 76%, and the operation is simple, the purity is high, and the application can provide a simple and efficient way for chemical synthesis of 6-HHS.