Plant-source antifungal composition and application thereof in cosmetics

Abstract

The invention discloses a plant-derived antifungal composition and application thereof in cosmetics, and belongs to the field of biological antibiosis, the composition takes pinosine (P) and sanguinarine (S) as composition components, and by accurately controlling the mass ratio of the pinosine (P) to the sanguinarine (S) to be (1-4): 1 and the solid-to-liquid ratio to be 0.002%-0.01%, the performance limitation of a single component is effectively overcome, and synergistic interaction is realized; experiments prove that under a specific ratio, the compound has a strong inhibition effect on various fungi and bacteria, such as staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, candida albicans and aspergillus niger, and has outstanding antifungal, antibacterial and antiseptic capabilities. The solvent of the composition accords with cosmetic regulations, can be prepared into various cosmetic dosage forms such as essence, emulsion and cream, and is widely applied to the field of cosmetics.

Description

Technical Field

[0001] The present invention belongs to the field of biological antibacterial, and particularly relates to a plant-derived antifungal composition and its application in cosmetics. Background Art

[0002] In recent years, the cosmetics industry has paid increasing attention to the issue of anti-corrosion. According to the current relevant regulations on cosmetics, its microbial detection indicators cover three types of bacteria and two types of fungi. The bacteria to be detected include Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa; the fungal detection mainly targets Candida albicans of the genus Candida and Aspergillus niger of the genus Aspergillus. Compared with bacteria, the cell wall composition and structure of fungi are different, which makes it more difficult for preservatives to penetrate and play a role. Especially for the genus Candida, yeast has diverse metabolic pathways and strong adaptability to adverse environments. When exposed to preservatives, yeast can adjust its own metabolism to resist the influence of preservatives. In contrast, most bacteria have weaker coping abilities in this regard. Taking the common cosmetics preservative benzoic acid as an example, it has a good inhibitory effect on bacteria such as Escherichia coli, but a poor inhibitory effect on yeast such as Candida albicans. It should be noted that its representative Candida albicans, as a conditional pathogenic fungus, is extremely harmful to the skin. It usually exists in parts of the human body such as the skin surface, oral cavity, and intestine. When the skin microecological balance is disrupted, such as using inappropriate cosmetics, damaged skin barrier, or decreased human immunity, Candida albicans may multiply in large numbers and cause skin infections. In the initial stage of infection, the skin may show symptoms such as erythema and itching. As the condition develops, the area of erythema will gradually expand, and the skin surface may also show desquamation and blisters. In severe cases, erosions will form, which not only affects the beauty of the skin but also brings great pain to the patient. If the infection spreads throughout the body, it may cause more serious health problems.

[0003] At present, consumers' attention to the green safety of cosmetics is increasing day by day. Therefore, natural plant-derived preservatives with both efficacy and anti-corrosion effects are highly favored. Such preservatives are derived from natural plants, which can not only effectively play the role of anti-corrosion but also highly meet consumers' pursuit of green and safe cosmetic ingredients, showing extremely promising application potential and broad market prospects in the cosmetics industry.

[0004] However, for plant crude extracts, even if they have anti-corrosion functions, due to their complex components, in the actual application process, they are often significantly affected by factors such as plant origin differences and extraction efficiency fluctuations, making it difficult to ensure the stability of the anti-corrosion function between batches. This problem seriously restricts the wide application of plant crude extracts in the field of cosmetics anti-corrosion. Summary of the Invention

[0005] The purpose of this section is to outline some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section, as well as in the abstract and title of the present application, to avoid obscuring the purpose of this section, the abstract, and the title. However, such simplifications or omissions shall not be used to limit the scope of the present invention.

[0006] In view of the above and / or problems existing in the prior art, the present invention is proposed.

[0007] Therefore, an object of the present invention is to overcome the deficiencies in the prior art and provide a plant-derived antifungal composition.

[0008] To solve the above technical problems, the present invention provides the following technical solution: a plant-derived antifungal composition, comprising pinosylvin and sanguinarine, wherein, by mass ratio, the mass ratio of pinosylvin to sanguinarine is 1-4:1.

[0009] As a preferred embodiment of the plant-derived antifungal composition of the present invention, further comprising a solvent, the solvent comprising pure water, 1,4-butanediol, 1,2-pentanediol, 1,2-hexanediol, and PEG-40 hydrogenated castor oil.

[0010] As a preferred embodiment of the plant-derived antifungal composition of the present invention, the mass ratio of the total mass of pinosylvin and sanguinarine to the mass of the solvent is 0.002-0.01:100.

[0011] As a preferred embodiment of the plant-derived antifungal composition of the present invention, the mass ratio of pinosylvin to sanguinarine is 2:1, 4:1, 1:1.

[0012] As a preferred embodiment of the plant-derived antifungal composition of the present invention, the mass ratio of pinosylvin to sanguinarine is 1:1.

[0013] As a preferred embodiment of the plant-derived antifungal composition of the present invention, the plant-derived antifungal composition has an inhibitory effect on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger.

[0014] Another object of the present invention is to overcome the deficiencies in the prior art and provide an application of a plant-derived antifungal composition in the preparation of cosmetics.

[0015] As a preferred embodiment of the application of the present invention, it includes adding the plant-derived antifungal composition to cosmetics to obtain the cosmetics.

[0016] As a preferred embodiment of the application of the present invention, the addition amount of the composition in the cosmetics is 1.58%-3.15% by mass fraction.

[0017] As a preferred embodiment of the application of the present invention, wherein: the cosmetics include essence lotion, emulsion, and cream.

[0018] Advantages of the present invention:

[0019] (1) Through scientific formulation, the present invention successfully invented a composition with excellent antifungal and antibacterial preservation functions. With pinosylvin (P) and sanguinarine (S) as the core components, precisely regulating the mass ratio and solid-liquid ratio of the two effectively overcomes the limitations of single-component and poor effect in current antifungal preservatives, realizes the synergistic effect between components, and significantly improves the comprehensive performance of the composition.

[0020] (2) The present invention is not a simple combination of components, but is verified through rigorous experiments. Experimental data show that under specific mass ratio and solid-liquid ratio conditions, the composition exhibits a strong inhibitory effect on a variety of fungi and bacteria. Its antifungal and antibacterial preservation ability scores far exceed those of similar products, showing a significant synergistic enhancement effect; moreover, by selecting solvents that comply with cosmetic regulations, a stable dosage form suitable for various cosmetics such as essence lotion, emulsion, and cream can be prepared, and it can be widely applied to the fields of antifungal and antibacterial preservation of cosmetics.

[0021] (3) The composition provided by the present invention has a simple and easy preparation process. The raw materials are natural pinosylvin and sanguinarine, which are safe, non-irritating, and have no toxic side effects. It not only ensures the efficacy of the product but also meets consumers' pursuit of safe and natural cosmetic ingredients, and has extremely high promotion and application value. Description of the Drawings

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings. Among them:

[0023] Figure 1 It is the time-kill curve graph of Candida albicans in Example 2 of the present invention;

[0024] Figure 2 It is the morphological observation graph of Candida albicans observed by scanning electron microscope in Example 2 of the present invention;

[0025] Figure 3 It is the synergistic efficacy graph of pinosylvin (P) and sanguinarine (S) combined against C.a in Example 3 of the present invention;

[0026] Figure 4This is the inhibitory effect diagram of the combined use of pinosylvin (P) and sanguinarine (S) on Candida albicans biofilm in Example 3 of the present invention;

[0027] Figure 5 This is the erythrocyte hemolysis rate diagram of pinosylvin (P) and sanguinarine (S) in Example 4 of the present invention. Detailed implementation manners

[0028] To make the above objects, features, and advantages of the present invention more obvious and understandable, the following will describe the detailed implementation manners of the present invention in combination with the embodiments of the specification.

[0029] In the following description, many specific details are set forth to fully understand the present invention. However, the present invention can also be implemented in other ways different from those described herein. Those skilled in the art can make similar generalizations without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0030] Secondly, the so-called "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that can be included in at least one implementation manner of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment that excludes other embodiments.

[0031] The raw materials in the present invention are all ordinary commercially available products. Pinosylvin and sanguinarine were both purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Pinosylvin (purity ≥ 97%, product number P168709), sanguinarine (purity ≥ 97%, product number S303178).

[0032] Example 1

[0033] Screening of plant-derived monomeric compounds:

[0034] Strains: Bacteria: Staphylococcus aureus (S.a, BNCC 186158), Escherichia coli (E.c, BNCC186653), Pseudomonas aeruginosa (P.a, BNCC 337005);

[0035] Fungi: Candida albicans (C.a, BNCC 336485), Aspergillus niger (A.n, BNCC 336539) were all purchased from Beijing NaChuangLian Biotechnology Co., Ltd.;

[0036] Preparation of bacterial suspensions: Bacteria were cultured in LB medium at 37 °C, and fungi were cultured in YPD medium at 30 °C. The bacterial suspensions in the logarithmic growth phase were transferred to centrifuge tubes, centrifuged, and the supernatant was removed and then redispersed in the liquid medium. After resuspending twice in this way, the bacterial suspensions of the five bacteria were obtained and reserved for use.

[0037] Preparation of samples: Five stilbene-like structures and five alkaloids, all from plant monomer components, were selected for screening. Additionally, two positive reference standards ( Figure 1 ) were added for comparison. All were dissolved in 50% butanediol, and the concentration after dissolution was 10 mg / mL to obtain the stock solution of the experimental samples for use.

[0038] This screening was carried out using the minimum inhibitory concentration (MIC) method: The minimum inhibitory concentration was determined by the micro-dilution method. The samples were serially diluted two-fold in a 96-well plate, and then the diluted bacterial suspension was added so that each well contained 10 6 CFU / mL of bacteria, 100 μL of the sample dilution (the sample was first dissolved in DMSO to prepare the stock solution and diluted with water to the required concentration during use, with DMSO content ≤ 5%) and 100 μL of the bacterial suspension in each well, with a total volume of 200 μL.

[0039] The 96-well plate was placed in an incubator. Bacteria were cultured at 37 °C for 24 h, and fungi were cultured at 30 °C for 24 h. The lowest concentration of the sample without visible bacterial growth was taken as the MIC, and three parallel experiments were performed each time.

[0040] The results showed that among the stilbene substances, pinosylvin exhibited significant antibacterial activity and a broad-spectrum antibacterial effect;

[0041] Among the alkaloid substances, sanguinarine (except against P.a) also showed good antibacterial effects. And the antibacterial ability of these two compounds against C.a and A.n of fungi was the most significant compared to other compounds. Therefore, pinosylvin (P; Pinosylvin) and sanguinarine (S; Sanguinarine) were used as the composition below.

[0042] Table 1. Primary screening of natural antibacterial compounds

[0043]

[0044] Note: ND = Not determined. All the above compounds were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0045] Example 2

[0046] Determination of the synergistic efficacy of the composition against C.a:

[0047] (1) Time-kill curve of the composition against C.a

[0048] To explore whether the combination of P and S has the possibility of synergistic anti-C.a, the time-kill curve against C.a was carried out.

[0049] Add different concentrations of the sample and diluted Candida albicans suspension into centrifuge tubes; among them, the sample is first dissolved in DMSO to prepare a stock solution, and diluted to the required concentration (aqueous solutions containing 5% DMSO at 200 μg / mL and 100 μg / mL) when in use;

[0050] After Candida albicans is cultured to the logarithmic growth phase, the cells are collected by centrifugation, and the microorganism is redispersed into the liquid medium to prepare a bacterial suspension with a concentration of about 2×10 6 ;

[0051] Add different sample solutions and an equal volume of the bacterial suspension into centrifuge tubes respectively.

[0052] Incubate in a shaker at 30 °C. At the specified time (within 24 hours), further incubate the diluent in the above test tubes with YPD agar medium for 48 hours;

[0053] The cell density of each sample is estimated by colony counting;

[0054] Time-kill curve tests of C.a cells treated with various samples and their combinations of P and S, and the control group are performed.

[0055] See Figure 1 (where P-2 is 100 μg / mL pinosylvin, S-2 is 100 μg / mL sanguinarine, P-1 is 50 μg / mL pinosylvin, and S-1 is 50 μg / mL sanguinarine). It is found that the number of cells in the control group (without antibacterial agent) reaches about 10 8 CFU / mL at 24 h, while the number of cells treated with P or S is lower than that of the control group.

[0056] Moreover, for the cells treated with P alone, there is an obvious trend of significant decrease. Compared with the initial cell number, P-2MIC only decreases by three orders of magnitude. In addition, the drug combination shows a significant bactericidal effect after 2 h. However, P alone only starts to show a bactericidal effect after 10 h of treatment. S alone only shows the effect of inhibiting growth and reproduction;

[0057] The bactericidal efficiency is improved after treatment with the P and S combination; after 24 h, its antibacterial rate against C.a reaches 99.99%, which indicates that there is a synergistic antifungal effect between P and S.

[0058] (2) Morphological observation by scanning electron microscope

[0059] Culture Candida albicans cells with the P and S combination and the control respectively. After 24 h, take the cells, rinse them twice with phosphate buffered saline (PBS, pH = 7), fix them on the carrier by freeze-drying, sputter gold in an argon environment, and observe the cells with a scanning electron microscope (SEM, S-4800) at a magnification of 10000×.

[0060] The results are shown in Figure 2 , and it can be seen that, compared with the individual administration of P or S, the combination of P and S causes serious collapse, shrinkage, distortion, etc. on the surface of C.a. Morphologically, it proves the possibility of potential synergistic effects of the combination.

[0061] Example 3

[0062] Determination of the optimal synergistic ratio of the combination against C.a:

[0063] (1) Determination of the synergistic ratio by checkerboard experiment

[0064] Its synergistic effect against C.a was determined by the checkerboard method. In a 96-well plate, compound P was added to the first well and then diluted along the horizontal coordinate;

[0065] Subsequently, two consecutive dilutions of compound S were added to each row;

[0066] Then the prepared suspension of Candida albicans was added so that each well contained 10 6 CFU / mL of Candida albicans;

[0067] The microplate was incubated at 30 °C for 24 hours;

[0068] The optical density (OD) at 600 nm was detected by a microplate reader (SpectraMax M2).

[0069] MIC is defined as the lowest concentration at which turbidity can be observed (OD600 < 0.1). The fractional inhibitory concentration (FIC) is calculated by the following formula:

[0070] FIC index = MIC PS / MIC P +MIC SP / MIC S ;

[0071] MIC P is the MIC of the compound alone, MIC PS is the MIC of the combination of compound P and compound S, MIC S is the MIC of compound S alone, MIC SP is the MIC of the combination of compound S and compound P.

[0072] FIC ≤ 0.5 indicates a synergistic interaction, while a range of 0.5 to 1.0 indicates an additive interaction;

[0073] FIC values from 1.0 to 4.0 indicate no differential effect, while FIC > 4.0 indicates antagonistic interaction.

[0074] The synergistic or antagonistic effects of the drugs were analyzed using Combenefit software of the Bliss independence standard model.

[0075] As Figure 3 shown, when the combination of P-1 / 2MIC and S-1 / 4MIC or P-1 / 4MIC and S-1 / 2MIC completely inhibited Candida albicans, it indicated an additive effect on Candida albicans based on the FIC index, and the FIC values were both 0.75. And OD 600 values reflected the quantity of Candida albicans;

[0076] In addition, the absorbance of P-1 / 2MIC + S-1 / 2MIC (OD600 = 0.022) was lower than that of P-1MIC (OD 600 = 0.066) and S-1MIC (OD 600 = 0.089). This indicated that at the same concentration, the antibacterial effect of this combination was stronger than that of using P or S alone; that is, the combination of P and S was superior to using them alone.

[0077] In Figure 3 shown in the right figure of the Bliss independence detection standard model, the deeper blue meant a stronger synergistic effect. The results showed that at three P:S mass ratios (2:1, 4:1, and 1:1), the combination had a strong synergistic effect. Among them, the synergistic effect was the strongest when the combination was P-1 / 2MIC and S-1 / 4MIC, followed by P-1 / 2MIC + S-1 / 8MIC and P-1 / 4MIC + S-1 / 4MIC.

[0078] (2) Determining the synergistic ratio by crystal violet staining biofilm experiment

[0079] The obtained C.a solution was diluted to 2×10 6 CFU / mL with liquid medium;

[0080] 100 μL of the above bacterial suspension and 100 μL of the sample were added to each well of a 96-well plate, and the total volume was 200 μL;

[0081] Then, the inoculated microplate was incubated at 30 °C for 24 hours;

[0082] The biofilm was gently washed 3 times with sterile PBS, and then fixed with 2.5% glutaraldehyde for 2 hours. The sample was immersed in 0.1% crystal violet solution for 15 minutes for staining;

[0083] After that, it was rinsed 3 times with PBS to thoroughly wash away the excess crystal violet. Then, 150 μL of absolute ethanol was added for decolorization;

[0084] The formation of biofilm was reflected by measuring the OD590 in 100 μL of the supernatant. The calculation formula for the biofilm formation rate was as follows:

[0085] Biofilm production (%) = (ODsample - ODblank) / (ODcontrol - ODblank) × 100%

[0086] ODsample was the absorbance of the sample group treated with the sample, ODcontrol was the absorbance of the control group without the sample, and ODblank was the absorbance of the blank group with the sample / PBS but without bacteria.

[0087] Figure 4 The results of crystal violet staining for the determination of Candida albicans cell biofilm formation showed that, compared with the control group, the biofilm formation rates of each treatment group decreased, and decreased with the increase in drug concentration. When P and S were used alone against Candida albicans, they both had obvious inhibitory effects on Candida albicans biofilm at a concentration as high as 1 / 2 × MIC, and the biofilm formation rates were 43.78% and 20.88% respectively. The combination of P-1 / 4MIC + S-1 / 4MIC had a significant inhibitory effect on Candida albicans biofilm, and the biofilm formation rate was as low as 8.95%, which was significantly lower than that of P-1 / 2MIC or S-1 / 2MIC at equivalent concentrations. This indicated that P and S could jointly inhibit the formation of Candida albicans biofilm, and the combined use was more effective than the single use.

[0088] Example 4

[0089] In order to take into account the population with skin problems such as acne and wounds during application, the present invention conducted an erythrocyte hemolysis experiment to evaluate its safety.

[0090] The specific method was to wash 4% rabbit erythrocytes (purchased through the Titan Technology platform, product brand Sbjbio, numbered Sbjbio#RBC-RAB003-100mL) with PBS and centrifuge until the supernatant became clear.

[0091] 500 μL of rabbit erythrocytes and 500 μL of the sample (the sample solutions of different concentrations were prepared by dissolving pinosylvin and sanguinarine in 20% DMSO + 80% PBS to form 300 μg / mL pinosylvin solution and sanguinarine solution respectively, and then diluting) were added to the centrifuge tube.

[0092] 0.1% triton X-100, DMSO solution and PBS were used as positive, negative and blank controls respectively.

[0093] After incubating at 37 °C for 1 hour, the mixture was centrifuged at 1000 g for 5 minutes.

[0094] Subsequently, 200 μL of the supernatant was transferred into a 96-well plate;

[0095] Hemoglobin release was measured by determining its OD at 576 nm;

[0096] All experiments were performed in triplicate.

[0097] The hemolysis rate was calculated as follows: Hemolysis rate (%) = (ODsample - ODblank) / (ODpositive - ODblank) × 100%

[0098] ODsample is the absorbance of the sample group treated with the sample, ODcontrol is the absorbance of the control group without the sample, and ODblank is the absorbance of the blank group with the sample / PBS.

[0099] The results were as Figure 5 shown that at concentrations up to 150 μg / mL, P / S alone did not induce erythrocyte hemolysis, and the hemolysis rate was less than 10%. In contrast, when P and S were combined at mass ratios of 1:1 (50 μg / mL:50 μg / mL) and 2:1 (66.6 μg / mL:33.3 μg / mL), at a concentration of 100 μg / mL, the hemolysis rate was only close to 10%, within the safe range. After combination, the concentration causing hemolysis decreased, but it has been proven in the time-kill curve that all Candida albicans can be killed at the concentration of P-1MIC + S-1MIC. And from this result, it can also be found that there is a synergistic effect after the two are combined.

[0100] Example 5

[0101] Antiseptic challenge test:

[0102] Preparation of bacterial suspension: Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were cultured at 37 °C until the logarithmic growth phase;

[0103] The bacteria were diluted with 0.85% normal saline to ensure that the number of bacteria was about 10 8 CFU / mL. The above three kinds of bacterial suspensions were mixed in equal volumes and reserved;

[0104] Preparation of fungal suspension: Candida albicans was cultured at 30 °C until the logarithmic growth phase, and the bacteria were diluted to about 10 7 CFU / mL with sterilized 0.85% normal saline;

[0105] Aspergillus niger was inoculated on a solid medium and cultured at 30 °C for 5 days. The bacterial lawn was rinsed with sterilized 0.85% normal saline containing 0.05% Tween 80 and diluted to the number of bacteria about 10 7 CFU / mL. The two kinds of fungal suspensions were mixed in equal volumes and reserved.

[0106] Sample inoculation: Take the weighed cosmetics, add a mixed bacterial suspension or a mixed fungal suspension equivalent to about 1% of the weight of the cosmetics, and mix evenly. Ensure that in the sample with the added bacterial suspension, the bacterial content per gram of the sample is about 10 6 CFU / mL;

[0107] In the sample with the added fungal suspension, the fungal content per gram of the sample is about 10 5 CFU / mL. Place the sample at room temperature (22.5 ± 2.5)°C for storage.

[0108] Sample detection: Detect the number of colonies in the cosmetics on the 1st, 7th, 14th, 21st, and 28th days (judge whether the anti-corrosion composition is excessive on the 1st day);

[0109] Each time, take 1 portion of the sample to be tested, add it to 9 portions of SCDLP medium (neutralizer), mix well, neutralize at room temperature for 30 min ± 15 min, then perform serial dilution, and use TSA to culture at 37°C for 24 h to determine the total number of colonies, and use SDA to culture at 30°C for 48 h to determine the total number of fungi;

[0110] If molds and yeasts grow on TSA, the actual number of bacteria growth needs to subtract the number of molds and yeasts.

[0111] Evaluation criteria: In this experiment, referring to the CTFA method, if the total number of colonies in the sample with the added bacterial mixture decreases by 99.9% on the 7th day and shows no growth by the 28th day, and the total number of colonies in the sample with the added fungal mixture decreases by 90.0% on the 7th day and shows no growth by the 28th day, it is judged that the anti-corrosion effect is qualified.

[0112] Among them, if one of the bacteria and fungi fails to meet the above criteria, it is judged that the anti-corrosion effect of the cosmetics is unqualified;

[0113] And conduct an anti-corrosion challenge experiment by preparing lotion (pH = 6.4) according to Table 2 below. The composition of this invention is added as described in Table 3. The specific method:

[0114] Use 1,4-butanediol:1,2-pentanediol:1,2-hexanediol:PEG-40 hydrogenated castor oil = 3:2:1:0.3 as the solvent to dissolve pinosylvin (P): sanguinarine (S) = 2:1. Among them, in the composition, the concentration of pinosylvin is 7.94×10 -2 wt%, and the concentration of sanguinarine is 3.97×10 -2 wt%;

[0115] The final result of its anti-corrosion challenge is shown in Table 3, and the results of the anti-corrosion challenges against bacteria and fungi are shown in Tables 4 and 5 respectively.

[0116] Table 2 Formulation of lotion for anti-corrosion challenge

[0117]

[0118] Table 3. Addition amount of anti-corrosion composition

[0119]

[0120] Table 4. Results of anti-corrosion challenge against bacteria

[0121]

[0122] Table 5. Results of anti-corrosion challenge against fungi

[0123]

[0124]

[0125] Through in-depth research and exploration, the inventors of the present invention innovatively proposed to use a complex of plant monomer components to achieve the anti-corrosion effect. This technical solution is expected to break through the limitations of existing plant-derived preservatives, provide a more stable, efficient and green-safe anti-corrosion solution for the cosmetics industry, and effectively resist the invasion of microorganisms such as Candida albicans on the skin.

[0126] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered within the scope of the present invention.

Claims

1. A plant-derived antifungal composition, characterized in that: include, Pinus erythrogenin and sanguinarine, wherein, by mass ratio, the mass ratio of pinus erythrogenin to sanguinarine is 1 to 4:

1.

2. The botanical antifungal composition according to claim 1, characterized in that: Also included are solvents including purified water, 1,4-butylene glycol, 1,2-pentanediol, 1,2-hexanediol, and PEG-40 hydrogenated castor oil.

3. The botanical antifungal composition according to claim 2, characterized in that: The mass ratio of the total mass of pinus erythrorhizon and sanguinarine to the solvent is 0.002-0.01:

100.

4. The botanical antifungal composition according to any one of claims 1 to 3, characterized in that: The mass ratios of pinus erythritol and sanguinarine are 2:1, 4:1 and 1:

1.

5. The botanical antifungal composition according to claim 4, characterized in that: The mass ratio of pinus erythritol to sanguinarine is 1:

1.

6. The botanical antifungal composition according to any one of claims 1 to 3 and 5, characterized in that: The plant-derived antifungal composition has an inhibitory effect on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger.

7. Use of the plant-derived antifungal composition according to any one of claims 1 to 6 in the preparation of cosmetics.

8. The use according to claim 7, characterized in that: The method comprises adding the plant-derived antifungal composition into cosmetics to prepare the cosmetics.

9. The use according to claim 7, characterized in that: The added amount of the composition in cosmetics is 1.58% to 3.15% by mass.

10. The use according to claim 8, characterized in that: The cosmetics include essence water, lotion and cream.

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