1915 results about "Staphyloccocus aureus" patented technology

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and / or Escherichia coli and / or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and / or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

An apparatus and methods for binding an analyte of interest in a sample are provided. The apparatus comprises a substrate with an exposed surface with an compound, that is electrostatically charged or capable of forming hydrogen bonds, provided bound to the solid substrate. A recombinant single chain antibody (scFv) molecule specific for the analyte of interest, having one or more amino acids with charged or hydrogen-bond forming sidechains in a linker polypeptide portion, is bound to the layer on the solid substrate. When the analyte of interest is present in the sample the scFv binds the analyte to the solid substrate. The apparatus can be used with an immunoglobulin layer to detect Fc receptors, so as to detect microorganisms such as Staphylococcus aureus having protein A or protein G.

The invention discloses a preparation method of a staphylococcus aureus CRISPR / Cas9 system and an application of the system in constructing a genetically modified mouse model. The staphylococcus aureus CRISPR / Cas9 system is composed of two components, namely Cas9 mRNA and gRNA, wherein a preparation method of the Cas9 mRNA is achieved by adding a T7 promoter to the upstream region of original Cas9 coding DNA, and a preparation method of the gRNA is achieved by adding the T7 promoter to the upstream region of an original gRNA coding sequence. The staphylococcus aureus Cas9 mRNA and gRNA, which are injected to mouse fertilized embryos through micro-injection, can achieve gene editing and modification of various types, such as single-gene knockout, multi-gene knockout and / or gene knock-in and the like, on the mouse fertilized embryos; therefore, the CRISPR / Cas9 system has a good application prospect in the aspects of fertilized embryo gene editing and modification of such animals as mouse and the like as well as construction of animal models.

The present invention is a substantially purified sortase-transamidase enzyme from Gram-positive bacteria, such as Staphylococcus aureus. The enzyme having a molecular weight of about 23,539 or about 29,076 daltons and catalyzing a reaction that covalently cross-links the carboxyl terminus of a protein having a sorting signal to the peptidoglycan of a Gram-positive bacterium, the sorting signal having: (1) a motif of LPX3X4G therein; (2) a substantially hydrophobic domain of at least 31 amino acids carboxyl to the motif; and (3) a charged tail region with at least two positively charged residues carboxyl to the substantially hydrophobic domain, at least one of the two positively charged residues being arginine, the two positively charged residues being located at residues 31-33 from the motif, wherein X3 is any of the twenty naturally-occurring L-amino acids and X4 is selected from the group consisting of alanine, serine, and threonine, and wherein sorting occurs by cleavage between the fourth and fifth residues of the LPX3X4G motif. Variants of the enzyme, methods for cloning the gene encoding the enzyme and expressing the cloned gene, and methods of use of the enzyme, including for screening for antibiotics and for display of proteins or peptides on the surfaces of Gram-positive bacteria, are also disclosed.

A method and composition for the passive immunization of patients infected with or susceptible to infection from Staphylococcus bacteria such as S. aureus and S. epidermidis infection is provided that includes the selection or preparation of a donor plasma pool with high antibody titers to carefully selected Staphylococcus adhesins or MSCRAMMs, or fragments or components thereof, or sequences with substantial homology thereto. The donor plasma pool can be prepared by combining individual blood or blood component samples which have higher than normal titers of antibodies to one or more of the selected adhesins or other proteins that bind to extracellular matrix proteins, or by administering carefully selected proteins or peptides to a host to induce the expression of desired antibodies, and subsequently recovering the enhanced high titer serum or plasma pool from the treated host. In either case, the donor plasma pool is preferably purified and concentrated prior to intravenous introduction into the patient, and the present invention is advantageous in that a patient can be immunized against a wide variety of potentially dangerous staphylococcal infections. Kits for identifying potential donor with high titers of the selected adhesins are also provided. The present invention thus provides methods and compositions which can be highly effective against infections associated with Staphylococcus bacteria.

The invention discloses a traditional Chinese medicine inclusion compound with an antibacterial effect and a textile finishing method of the traditional Chinese medicine inclusion compound. The traditional Chinese medicine inclusion compound is prepared from traditional Chinese medicine extract liquid with the total ingredient percentage being 100 percent and beta-cyclodextrin through a saturated water solution method, wherein the traditional Chinese medicine extract liquid comprises the following ingredients in percentage by mass: 25 percent to 60 percent of honeysuckle, 15 percent to 50 percent of folium Artemisiae argyi, 20 percent to 35 percent of liquorice and 5 percent to 15 percent of scutellaria baicalensis. The traditional Chinese medicine inclusion compound and cross linking agents are added into chitosan acetic acid solution to be prepared into antibacterial finishing liquid. A simple rolling baking process is adopted for carrying out antibacterial finishing on the cellulose fiber, the process is simple, the finished textile has the broad-spectrum antibacterial performance on common germs such as staphylococcus aureus, escherichia coli and the like, and in addition, the water washing resistance performance is good.

The invention discloses sgRNA for specific targeting of a human CXCR4 gene, a sgRNA-containing staphylococcus aureus CRISPR / SaCas9 system and a method for specific human CXCR4 gene knockout by the method. A target sequence of sgRNA is shown as any one of SEQ ID NO:1-4. sgRNA for specific targeting of the human CXCR4 gene and AAV-CRISPR / SaCas9 plasmids are connected to form a carrier and packaged into AAV infection cells, simplicity, convenience, high efficiency and specificity in CXCR4 gene knockout are realized, and accordingly the problem of limitation in adoption of SpCas9 targeted CXCR4 for treatment of acquired immune deficiency syndrome is effectively solved.

Disclosed herein are isolated and purified Staphylococcus aureus bi-component leukocidin, referred to herein as LukAB, and its components LukA and LukB, antibodies specific to LukA, antibodies specific to LukB, therapeutic compositions containing LukA and / or LukB, or anti-LukA and / or anti-LukB antibodies, uses of the compositions to treat acute inflammatory conditions or S. aureus infection, methods for identifying inhibitors of LukAB-mediated cytotoxicity of human phagocytes, and methods for using LukAB as a marker to predict severity of S. aureus infection.

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and / or Escherichia coli and / or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and / or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

A method and composition for the passive immunization of patients infected with or susceptible to infection from Staphylococcus bacteria such as S. aureus and S. epidermidis infection is provided that includes the selection or preparation of a donor plasma pool with high antibody titers to carefully selected Staphylococcus adhesins or MSCRAMMs, or fragments or components thereof, or sequences with substantial homology thereto. The donor plasma pool can be prepared by combining individual blood or blood component samples which have higher than normal titers of antibodies to one or more of the selected adhesins or other proteins that bind to extracellular matrix proteins, or by administering carefully selected proteins or peptides to a host to induce the expression of desired antibodies, and subsequently recovering the enhanced high titer serum or plasma pool from the treated host.

The invention relates generally to novel N-thiolated β-lactams. More specially, the invention relates to the use of these novel antibacterial agents in the treatment or inhibition of methicillin-resistant Staphylococcus aureaus.

Described herein are novel SCCmec right extremity junction (MREJ) sequences for the detection and / or identification of methicillin-resistant Staphylococcus aureus (MRSA). Disclosed are methods and compositions based on DNA sequences for the specific detection of MREJ sequences designated types xi, xii, xiii, xiv, xv, xvi, xvii, xviii, xix, and xx for diagnostic purposes and / or epidemiological typing.

The invention discloses a nano-silver loaded activated carbon fiber and a preparation method thereof. The preparation method comprises the following steps: (1) taking AgNO3 or CH3COOAg as a silver source; (2) utilizing a mixed solution of stearic acid, lauric acid and polyvinylpyrrolidone as a protecting agent; (3) taking the mixed solution of glycol, formaldehyde and glucose as a reducing agent; (4) fully dipping an activated carbon fiber in the AgNO3 or CH3COOAg solution, thereby loading silver ions on the surface of the activated carbon fiber; and (5) in an acetone system, utilizing the reducing agent to reduce the silver ions on the surface of the activated carbon fiber, thereby generating nano-silver particles on the surface of the activated carbon fiber. The product disclosed by the invention is characterized by adjustable silver loading capacity, monomer silver particle diameter of 10-20 nanometers, application to the filtering and sterilizing technology for drinking water and efficient function of killing escherichia coli and staphylococcus aureus in water. Besides, the nano-silver loaded activated carbon fiber has higher application value in improving water chrominance and turbidity and reducing residual chlorine in water.

The invention discloses a polylactic acid antibacterial nanofiber membrane and a preparation method thereof, which belong to the technical field of functional spinning. An antibacterial agent is added into polylactic acid, and an electrostatic spinning technology is adopted to prepare the polylactic acid antibacterial nanofiber membrane with high antibacterial activity. The antibacterial agent in the fiber membrane is triclocarban (TCC). The nanofiber membrane comprises 95 or 94 mass percent of polylactic acid and 5 or 6 mass percent of antibacterial activity TCC. The obtained antibacterial fiber membrane can inhibit over 93 percent of staphylococcus aureus, escherichia coli and candida albicans. The antibacterial fiber membrane can be applied to the fields of daily use, spinning, industry and medicaments.

The invention discloses a bacillus velezensis strain CSQXDZ26, which is bacillus velezensis (Bacillus velezensis) with the preservation number of CGMCC NO. 17935. The bacillus velezensis CSQXDZ26 hasremarkable antagonistic activity on bacterial plant diseases such as Xanthomonas oryzae pv. Oryzae, Xanthomonas oryzae pv. oryzicola, and xanthomonasaxonopodis pv. glycines AND THE LIKE; meanwhile, the bacillus velezensis CSQXDZ26 also has antibacterial activity on fungal plant diseases such as dithiorella gregaria, Fusarium graminearum, Fusarium oxysporum f.sp.Cucumerinum, Fusarium oxysporum f.sp. momordicae, Alternaria solani or Alternaria alternate, and also has excellent inhibition effect on common food spoilage bacteria such as bacillus subtilis, proteusbacillus vulgaris and staphylococcus aureus.

The invention provides an N, P and S-codoped fluorescent carbon quantum dot and a preparation method and application thereof, and belongs to the technical field of luminescent nanomaterials. The preparation method comprises the steps that a biological bacterium (saccharomycete or escherichia coli or staphylococcus aureus or aspergillus niger) is taken as a carbon source, water of the corresponding volume is added, and a hydrothermal reaction is performed to obtain a dark brown solution; after the reaction stops and a reaction kettle is naturally cooled, dialysis is performed to remove impurities, an aqueous carbon quantum dot solution is obtained, and after freeze drying is performed, the N, P and S-codoped fluorescent carbon quantum dot is obtained. According to the method, the biomass is taken as the carbon source, the raw material is wide in source, low in cost, green and environmentally friendly, the technology is simple, and the preparation condition requirement is low. The obtained carbon quantum dot can be applied to detection on Cr (VI) ions, MnO4<-> and ascorbic acid by serving as a switch-type fluorescent probe and also can be used for cell imaging.

One finishing solution of fabrics containing chitosan is composed of Chitosan (MW <= 5000, the degree of deacetylation of >= 90%, 1.00 - 5.00%), acetic acid (0.50 - 6.00%), epichlorohydrin (0.01 - 1.00%) and water. The process for the fabric finishing is: I,fabric Pretreatment: fabric by the alkali treatment (8 - 11% NaOH at room temperature), washed, acid and (2 - 4% acetic acid at room temperature), drying (85 - 100 deg.C). II,finishing: baptist, rolling (room temperature, 15 to 25 min, rolling rate of more than 80 - 90%), alkali (room temperature, 5 - 8% NaOH), acid and (room temperature, 1 to 3% acetic acid) and Drying (85 - 100 deg.C). The test results show that the inhibitory rate for staphylococcus aureus, Escherichia coli, Streptococcus white Nian is more than 99% which is the higher level compared to the state 3-A class standard. The fastness of fabrics reaches 4 after washing.

Staphylococcus aureus Hla polypeptides having various deletions, insertions, and / or mutations which are useful for immunisation are provided herein. Also provided herein are Hla heptamers which are non-haemolytic. Additionally, an effective Staphylococcus aureus vaccine may require several antigenic components, and so various combinations of S. aureus antigens, including Hla polypeptides having deletions, insertions, and / or mutations, are identified for use in immunisation. These polypeptides may optionally be used in combination with S. aureus saccharides.

A method and apparatus for preventing and treating septicemia in patient blood is provided. The extracorporeal system includes an antimicrobial device to inactivate at least 99% of bloodborne microorganisms, a hemoconcentrator / filtration unit to remove approximately 50-75% of target molecules from the patient blood and a filter unit to remove target molecules from patient blood from the sieved plasma filtrate. Target molecules are produced by microorganisms, as well as by the patient's cells. These molecules include endotoxins from Gram negative bacteria, exotoxins from Gram negative and Gram positive bacteria, as well as RAP protein mediator from Staphylococcus aureus, and cell mediators such as tumor necrosis factor-alpha, and interleukin 1-beta, interleukin 6, complement proteins C3a and C5a, and bradykinin.

The present invention provides methods to engineer microorganisms for the production of C30-aldehyde carotenoids. Specifically, various combinations of crtM, sqs, crtN and crtN2 genes from Staphylococcus aureus and Methylomonas sp. 16a can be co-expressed in transformant hosts, leading to the production of diaponeurosporene monoaldehyde, diapocarotene monoaldehyde, and / or diapocarotene dialdehyde. In a preferred embodiment, the genetically engineered pathway is introduced into a strain of Escherichia coli that has been engineered for the expression of carotenoids, and the C30-carotenoid product is diapocarotene dialdehyde.

The present invention discloses one plant lactobacillus strain and its application. The plant lactobacillus, Lactobacillus plantarum L323 CGMCC No. 1329, is separated from Chinese pregnant woman's vaginal secretion, and has relatively high bacteriotasis on common vaginal pathogens, such as staphylococcus aureus, candida albicans, colibacillus and vaginal Gardnar bacillus, and acid producing and H2O2 producing capacity higher than other lactobacillus. The plant lactobacillus, Lactobacillus plantarum L323 CGMCC No. 1329, may be used in preparing vaginal microbial preparation for preventing and / or treating vaginal infectious diseases.

The invention relates to the technical field of daily chemical products and in particular to a clean effervescent tablet with pesticide residue and bacteria removing effects and a preparation method of the clean effervescent tablet. According to the preparation method disclosed by the invention, a specific surfactant is combined with a special wet granulation process, so that the feasibility of the production process is ensured. The clean effervescent tablet has the advantages that no dryness or stickness is caused; the adhesion of foam to the surface of a disintegrating agent can be inhibited, the disintegration speed is increased and an effervescent effect is quickly exerted; in the disintegration and dispersion process, the efficient alkaline supplementing effect on a solution system is realized by synergizing a bacteria remover with an alkali-source supplement, fruit wax and pesticide left on vegetables and fruits can be effectively removed, and the aims of effectively removing escherichia coli and staphylococcus aureus are achieved. By using the alkali-source supplement with a special structural form, metal ions in the water body and on the surface of the vegetables and the fruits can be effectively chelated, the effects of softening the water body and chelating the metal ions are realized, and the deposition of calcium scales on the surfaces of cleaned materials is prevented; in addition, a variety of calcium scales or stains are dispersed, so that secondary pollution to tableware, the fruits and the vegetables caused by deposition is prevented.

The invention discloses an antibacterial polymer material which is polymerized by a plurality of components including antibacterial oligomer. The specific components comprise 30-99.5wt percent of unsaturated double bond monomer, 0.01-50wt percent of unsacturated double bond antibacterial oligomer, 0-10wt percent of hydrophilic monomer, 0.01-5.0wt percent of polymerization initiator and 0-80wt percent of fillings. The antibacterial polymer material of the invention is safe to the health of people and has long antibacterial performance and the antibacterial rates of the invention to staphyloccocus aureus rosenbach and escherichia coli are both more than 99percent. The invention is applicable for medical treatment and hygiene industries, including the wound dressing material, dental recovery materials used in dentistry (such as tooth bonder, tooth recovery coating, tooth recovery substrate and tooth filling materials, etc.), surgery seam and materials for daily use, etc.

The invention relates to the field of biology, particularly to novel Staphylococcus aureus phages and a composition thereof, and applications of the composition, and provides 4 Staphylococcus aureus phages such as Podoviridae sp .BP-13 with the preservation number of CCTCC NO:M2015142, Staphylococcus aureus phage BP-13A with the preservation number of CCTCC NO:M2016535, Podoviridae sp .BP-14 withthe preservation number of CCTCC NO:M2015143, and Podoviridae picovirinae Ahjdlikevirus BP-39 with the preservation number of CCTCC NO:M2015144. According to the present invention, the four phages arethe strict potent phages, can effectively kill various types of Staphylococcus aureus such as MRSA, MSSA, BORSA and the like, have wide host ranges, and have no toxic effect on normal microbial flora, and the DNA of the phages cannot encode virulence genes, such that the Staphylococcus aureus phages can be used for preventing or treating human and animal bacterial infections caused by Staphylococcus aureus, can provide excellent strain resources for the development of novel antibacterial preparations, and have good application development prospects; and the host range and the antibacterial efficiency of the cocktail mixture of the present invention are further greatly improved.

The invention discloses lactobacillus plantarum CQ02-108 and application thereof to preparation of fermented sausages. The lactobacillus plantarum CQ02-108 is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 17, 2018; and the preservation number is CGMCC No. 16121. The lactobacillus plantarum CQ02-108 can be used for producing proteinase and rapidly producing acid, does not cause viscosity, produce gas, produce biological amine, produce H2O2, produce H2S and produce pigments, and also has the advantages of high-salt concentration tolerance, high acidity tolerance, inhibition of growth of escherichia coli and staphylococcus aureus, bile salt resistance and gastric juice tolerance. When the lactobacillus plantarum CQ02-108 is used for fermenting the sausages, the quantity of viable bacteria of lactic acid bacteria in the fermented sausages can be remarkably improved and the pH (Potential of Hydrogen) value of the sausages is reduced; and the growth ofharmful bacteria is inhibited and the quality of the fermented sausages can be remarkably improved.

The invention discloses a preparation method for a silver-containing carbon dot and application of the silver-containing carbon dot to preparation of an antibacterial agent. The preparation method comprises the following steps: with polyethyleneimine (PEI), citric acid and silver nitrate as raw materials, synthesizing a silver-doped functionalized carbon dot solution (a PEI-Ag carbon dot solution)by using a hydrothermal process or microwave process; and then carrying out centrifugation and drying so as to obtain the solid PEI-Ag carbon dot. The preparation method provided by the invention isenvironment-friendly in preparation and uses cheap and easily available raw materials; and the prepared PEI-Ag carbon dot has good biocompatibility and high quantum yield, can be individually used orcooperatively used with antibacterial agents like tetracycline, gentamycin and cefoxitin, and show inhibitory effect on Staphylococcus aureus, Escherichia coli, Candida albicans and the like.

The invention discloses a water-based UV anti-bacterial coating. The water-based UV anti-bacterial coating comprises, by weight, 60-80 parts of urethane acrylate dispersoid, 5-15 parts of nano-silver collosol, 5-20 parts of reactive diluent, 3-6 parts of photoinitiator, 0.1-1 part of flatting agent, 0.1-1 part of dispersing agent and 0.1-1 part of antifoaming agent. The invention further provides a preparing method of the water-based UV anti-bacterial coating. In the water-based UV anti-bacterial coating and the preparing method thereof, the sterilization rate of the water-based UV anti-bacterial coating on escherichia coli and staphylococcus aureus can be 95% or above, and the anti-bacterial effect is good. By means of the water-based UV anti-bacterial coating, automatic assembly line coating can be achieved, construction is convenient, the curing speed is high, and broad application prospects are achieved.

The invention belongs to the field of biotechnology, and relates to a MntC recombinant protein of staphylococcus aureus (SA), a carrier comprising the recombinant protein, a host, a composition or a kit, application, preparation, fermentation and purification method of the protein. The MntC recombinant protein prepared by the method has strong immunogenicity, is safe and non-toxic, and is proved by animal tests to be able to effectively stimulate an organism to generate high efficient humoral immune response and good immune protection.