117 results about "Staphyloccocus aureus" patented technology

The application discloses a super-sensitive detection composition of aquatic product pathogenic bacteria and an application and a detection method thereof, and belongs to the technical field of biotechnology.The composition comprises an amplification primer with a sequence shown as SEQ ID No.1-2, a hairpin probe H1 with a fluorescent group and a quenching group modified on a nucleotide sequence shown as SEQ ID No.3, and a hairpin probe H2 with a sequence shown as SEQ ID No.4.Compared with conventional detection technologies such as a bacterial culture method, an instrument analysis method, a molecular biology method and an immunology method, the application provides a set of amplification primers and hairpin probes based on HCR, has higher specificity when detecting escherichia coli of aquatic products, and can avoid the influence of staphylococcus aureus, streptococcus pyogenes, pseudomonas aeruginosa and salmonella; meanwhile, the application has better sensitivity, and the detection limit can reach 0.4 CFU / mL; in addition, compared with other hairpin probes, the application can avoid false positives and improve reaction efficiency.

This invention relates to the field of rose separation and purification technology, specifically a novel benzofuran compound from rose petals, its extraction method, and its application. The method involves extracting the total rose extract obtained by heating and reflux of dried rose petals, then extracting the petroleum ether fraction. After elution and separation, the third fraction is further eluted with silica gel, the second fraction is eluted again, and the third fraction is purified by elution. The novel benzofuran compound from rose petals is obtained at 21.0 minutes. This invention discloses for the first time the extraction and isolation of a benzofuran compound from rose petals, and its antibacterial pharmacodynamic effects were tested. The experiments clearly showed that the compound has a strong inhibitory effect on Staphylococcus aureus and Escherichia coli, thus enabling its application in the preparation of antibacterial drugs and antibacterial health products.

The application provides a method and a kit for detecting Staphylococcus aureus. The method embeds multivalent binding probes on microspheres through strain-promoted azide-alkyne cycloaddition reaction, thereby forming microspheres loaded with multivalent binding probes. The microspheres loaded with multivalent binding probes are aggregated in a large size through multivalent binding between the probes and the genome of Staphylococcus aureus, so as to qualitatively and quantitatively identify Staphylococcus aureus through fluorescence. The method not only shortens the detection period and reduces the cost, but also has the advantages of high sensitivity and high specificity.

The invention belongs to the technical field of biological medicine, and particularly relates to a PDHC mutant, an immunogenic fragment and application, the PDHC mutant has amino acid mutation at at least one of the 214th site, the 274th site or the 294th site of the amino acid sequence shown in SEQ ID NO: 1, and the PDHC mutant is used for preventing or treating staphylococcus aureus (SA) infection and is an mRNA vaccine for preventing or treating staphylococcus aureus (SA) infection. The stability and immunogenicity of the staphylococcus aureus PDHC antigen are greatly improved, efficient targeted delivery of the antigen is achieved, and the immune effect of the vaccine is remarkably improved.

This invention relates to a method for simultaneously detecting *Salmonella typhimurium* and *Staphylococcus aureus* in chicken meat based on an instantaneous steady-state upconversion fluorescence sensor. First, a water-soluble upconversion material is encapsulated in a hollow cylinder to prepare an upconversion fluorescence cuvette. Next, a fluorescence sensor is constructed, comprising magnetic graphene oxide, aptamers specifically recognizing *Salmonella typhimurium* and *Staphylococcus aureus*, and enzyme modifications. By measuring the fluorescence signals, a detection standard curve for the two target bacteria is established. This method can determine the content of *Salmonella typhimurium* and *Staphylococcus aureus* in chicken meat. This invention improves the reusability of materials and the accuracy of detection results through the prepared upconversion fluorescence cuvette, simplifies the operation process and reduces costs by utilizing magnetic graphene oxide, and enhances the sensitivity of the sensor by utilizing enzyme catalysis. This detection method has a wide linear detection range and a low detection limit, showing promising application prospects.

The application provides a fluorescent rapid detection reagent and application thereof, and belongs to the technical field of bacterial detection.The fluorescent rapid detection reagent provided by the application comprises bacterial specific aptamer, priming chain and hairpin structures H1, H2 and H3.The application establishes a fluorescent specific amplification detection system with high specificity and sensitivity, develops fluorescent signal amplification labels for susceptible staphylococcus aureus and pseudomonas aeruginosa of rodents and handheld visual detection technology, and is prepared into a detection reagent, so that rapid and sensitive detection of target bacteria is realized, and the application has important significance for screening and detection of pathogenic microorganisms of experimental animals and quality control.

An AgCuTe nanowire composite material, its preparation method, and its application in medical antibacterial gauze are disclosed. The material consists of two phases: Cu7Te4 and Ag2Te, exhibiting a one-dimensional nanowire morphology with uniform silver distribution and nanowire diameters ranging from 7 to 9 nm. This material is prepared via a tellurium nanowire template combined with a two-step liquid-phase displacement reaction. First, tellurium nanowires are synthesized hydrothermally, followed by sequential reactions with copper and silver sources at room temperature to obtain the product. The AgCuTe nanowires exhibit concentration-dependent and power-dependent photothermal heating effects under 808 nm near-infrared light irradiation, with good photothermal stability. Under near-infrared light irradiation, the material demonstrates significant synergistic antibacterial activity against Staphylococcus aureus and Escherichia coli. Loading this material onto medical gauze can produce a near-infrared responsive photothermal antibacterial dressing for wound care and infection control, without antibiotic dependence, thus helping to reduce the risk of bacterial resistance.

This invention belongs to the field of biomedical functional materials technology, and relates to an antibacterial and healing-promoting photosensitizer, its preparation method, and its uses. The photosensitizer is a defective MIL-88B@MIL-88C composite material, wherein defective MIL-88B serves as the substrate, and MIL-88C grows on the surface and / or in the pores of the defective MIL-88B. This photosensitizer significantly improves the photogenerated carrier separation efficiency, reduces impedance, enhances photocurrent response, and can efficiently generate reactive oxygen species under light irradiation. Antibacterial experiments show that it has excellent bactericidal effects against Staphylococcus aureus, Escherichia coli, and methicillin-resistant Staphylococcus aureus, and is not prone to inducing drug resistance. Simultaneously, this photosensitizer has good biocompatibility, promotes cell migration, and has significant hemostatic function. Animal models show that it can effectively accelerate the healing of skin defects.

This application belongs to the field of biomaterials, specifically relating to an antibacterial polymeric derivative of F127 with terminal modification of acetyl bromide, its preparation method, and its application. This application obtains F127AcBr by terminal modification of polymeric F127 with acetyl bromide via an acylation reaction. Its good antibacterial effect and biocompatibility were verified in vitro using plate antibacterial assays against Staphylococcus aureus and Escherichia coli, as well as live / dead staining assays of L929 cells and HUVEC cells. This application optimized the concentration of F127AcBr and loaded it into an MS hydrogel system. Excellent biocompatibility was confirmed in a diabetic mouse wound model using HE staining of mouse organs. Immunofluorescence staining against Staphylococcus aureus further confirmed the good antibacterial activity of F127AcBr.

This invention belongs to the field of biomedical materials technology, and discloses a cellulose-based long-acting antibacterial material, its preparation method, and its application. The cellulose-based long-acting antibacterial material includes: a cellulose backbone; EDTA groups covalently grafted onto the cellulose backbone via ester bonds; and an ionic liquid anchored to the EDTA groups via multiple hydrogen bonds. The EDTA groups act as molecular bridges connecting the cellulose backbone and the ionic liquid, which remains in a monomeric state and is stably immobilized on the material surface through hydrogen bonds. This invention utilizes EDTA groups as molecular bridges to stably anchor the ionic liquid to the cellulose backbone surface through multiple hydrogen bonds, avoiding the problem of easy loss of physical load and overcoming the influence of covalent polymerization on antibacterial activity. After a 95-day accelerated aging test, the material still exhibits an antibacterial rate exceeding 99.9% against *Escherichia coli* and *Staphylococcus aureus*, meeting the needs of chronic wound care throughout the entire process.

The present invention belongs to the technical field of microorganisms, and particularly relates to a Lactobacillus plantarum NXU0011 and application thereof. The Lactobacillus plantarum NXU0011 is preserved in the China General Microbiological Culture Collection Center, with the preservation number of CGMCC NO: 26970. The bacterium has a good inhibition effect on Escherichia coli and Staphylococcus aureus. The Lactobacillus plantarum NXU0011 also has good antioxidant performance, and can be used for preparing hypoglycaemic drugs. At the same time, the Lactobacillus plantarum NXU0011 has a high survival rate in saliva, gastric juice or intestinal juice, and has the potential to become a good probiotic effect regulator.

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 9I and 16F replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-11. Compared to the template peptide PL-0, the stapling peptide PL-11 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

This invention discloses an antibacterial agent containing acrylic acid and a halogenated amine copolymer, a PVA antibacterial film containing the antibacterial agent, and a preparation method thereof. The antibacterial agent is synthesized by aqueous copolymerization of acrylic acid and halogenated amine monomers. The copolymer is then mixed with polyvinyl alcohol at a mass ratio of (2-10):5, stirred, coated onto a substrate, dried, and then sprayed with a neutralized dilute sulfuric acid and bleach mixture for chlorination. After standing and cleaning, the film is ready. This antibacterial agent possesses excellent water solubility and stability, overcoming the migration defects of small-molecule antibacterial agents. When blended with polyvinyl alcohol to prepare an antibacterial film, the intermolecular interactions significantly improve the flexibility and extensibility of the film material. Furthermore, it achieves a 100% bactericidal rate against Staphylococcus aureus and Escherichia coli within 10 minutes. It is suitable for antibacterial coating protection on solid and fabric surfaces, and is convenient and efficient to use.

The application provides a copper ion doped carbon nanotube composite material and a preparation method and application thereof, and belongs to the technical field of functional materials. In the composite material, copper ions are doped in the carbon nanotube skeleton in an atomic dispersion mode or a nano cluster form, the content of the copper ions in the composite material is 0.1-5wt%, the conductivity of the composite material is greater than or equal to 1.0*10 5 S / m, and the bacteriostatic rate on escherichia coli and staphylococcus aureus is greater than or equal to 98.5%. The composite material has excellent conductivity and good antibacterial property, good dispersibility in polymers such as PA6, and is not easy to agglomerate, and has good spinning performance.

This invention provides a recombinant Staphylococcus aureus enterotoxin B nanoparticle protein vaccine, mSEB-mi3, its preparation method, and its applications. This invention utilizes a SpyTag / SpyCather protein linker system to connect the recombinant protein to the nanoparticle protein, allowing mSEB to be displayed on the surface of self-contained nanoparticles, thus obtaining the recombinant Staphylococcus aureus enterotoxin B nanoparticle protein mSEB-mi3. Both mSEB-SpyTag and SpyCather-mi3 proteins in this invention are induced to be expressed in soluble form in Escherichia coli. The mSEB-SpyTag and SpyCather-mi3 of this invention can be covalently bound via isopeptide bonds to form the mSEB-mi3 nanoparticle protein vaccine. The binding conditions are simple, and when combined with the adjuvant MF59, it can induce rapid antibody production in mice, generating a high-level immune response and exerting a protective effect. Furthermore, its antibody response rate and protective efficiency are superior to those of the monomeric protein mSEB.

This invention relates to the field of fluorescent probe technology, specifically to a triphenylamine amphiphilic fluorescent probe, its preparation method, and its applications. The triphenylamine amphiphilic fluorescent probe is synthesized in three steps: bromination, coupling, and condensation to construct a D-π-A molecular structure, producing far-red light emission at 650-660 nm and exhibiting aggregation-induced emission (AIE) properties. The pyridine group endows it with hydrophilicity and cationic characteristics, while the triphenylamine group endows it with lipophilicity and AIE properties. It can specifically bind to bacterial surface anions, enabling wash-free, high-contrast real-time imaging, and can also disrupt bacterial biofilms to exert antibacterial effects. The probe has a MIC as low as 1.5625 μM against bacteria such as Staphylococcus aureus and Escherichia coli, can be co-stained with acridine orange for verification, is compatible with conventional detection equipment, and achieves integrated bacterial diagnosis and treatment, solving the problems of fluorescence quenching and low emission peaks in traditional probes.

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 6V and 13V replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-10. Compared to the template peptide PL-0, the stapling peptide PL-10 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

The application provides a sensor with antioxidant and antifouling properties and a preparation method and application thereof, and belongs to the technical field of marine chemistry. The sensor takes a glassy carbon electrode (GC) as a substrate, adopts polyaniline as a solid contact layer, and takes a calcium ion selective membrane (ISM) as a detection core. The innovation of the sensor is that a fucoidan-based functional polypeptide (PFp) is modified on the ISM to construct an antifouling layer. The PFp layer forms a triple synergistic defense mechanism of "anti-adhesion-sterilization-antioxidation". Experiments prove that the sensor can effectively inhibit the adhesion and colonization of bacteria such as escherichia coli and staphylococcus aureus and chlorella, and significantly slow down the oxidative decay of the sensing membrane. In addition, when the sensor is used for calcium ion detection in actual seawater, it exhibits excellent long-term stability and high precision, and provides an innovative strategy for continuous in-situ monitoring in complex media.

The application discloses five kinds of high-throughput detection primer groups, kits and application of LAMP method for foodborne pathogenic bacteria, and belongs to the technical field of foodborne pathogenic bacteria detection. The primer groups correspond to five kinds of bacteria, including Salmonella, Vibrio parahaemolyticus, Listeria monocytogenes, Escherichia coli O157:H7 and Staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No. 1-30. The complete process detection can be completed at 62 DEG C constant temperature for 45 minutes, the result does not need complex instruments, the positive gray and the negative purple are judged by naked eyes, and the operation is simple. The detection sensitivity reaches 1-75 copies / reaction, the artificially contaminated aquatic product sample does not need to be subjected to bacterial enrichment, is subjected to simple lysis and enrichment, has strong specificity and no cross reaction. The anti-interference ability is outstanding, the complex matrix of aquatic products is adapted, the detection coincidence rate is high, the cost is low, only a conventional constant temperature device is needed, and the application is suitable for multiple scenes, such as detection institutions, food enterprises and on-site law enforcement on-site detection of primary food, agriculture, disease control, customs and the like, and provides an efficient and economical technical means for aquatic product safety control.

The application discloses a kind of magnetic capture material based on phage display, preparation method and application thereof, through the synergistic effect of specific capture and short-time proliferation, the detection limit of staphylococcus aureus is improved to 10 4 CFU / mL, reaches the detection limit of portable ATP bioluminescence instrument itself, ensures the reliability of detection result. At the same time, the whole detection process can be completed within 1 hour, only needs simple equipment such as magnetic separation frame and portable ATP detector, easy to operate, fast, suitable for food safety on-site detection, medical institution environment monitoring and various scenes. In addition, compared with traditional antibody, phage-derived binding polypeptide can be produced in large quantities by microbial fermentation, with lower cost and better batch consistency, and the directional display strategy based on T4 capsid avoids the activity loss caused by chemical coupling, further improves the stability of reagent, and has good application and promotion prospect.

This invention provides an atypical tetracycline antibiotic derivative, its preparation method, and its application. The atypical tetracycline antibiotic derivative has the compound structure shown in Formula II: wherein, R 1’ Represents any one of H, amino group and its substituents or their hydrochloride salts, amine group, amide group, sulfonamide group, hydroxyl group and its substituents, and ester group; R 2’ Represents any one of H, alkyl, aryl, acyl, nitroso, amino, and amide. Based on a series of intermediates with structures shown in Formula I, this invention further synthesizes atypical tetracycline derivative structures with multiple site modifications. Cell activity tests show that they have varying degrees of inhibitory effects on *Escherichia coli* and *Staphylococcus aureus*, demonstrating potential for development into antibacterial therapies.

This invention provides a method for preparing and applying a long-lasting photocatalytic antifouling coating material, belonging to the field of marine antifouling coating technology. This invention uses an improved wet chemical reduction method to prepare an Ag@CuGaO heterojunction photocatalytic antifouling agent. This material is a nanocubic structure of CuGaO modified with Ag nanoparticles, with a silver loading of 0.5~10wt%. It combines the small size effect of Ga doping with the surface plasmon properties of Ag, which can broaden the light absorption range and improve carrier separation efficiency. 1~5 parts by weight of this antifouling agent are compounded with 100 parts by weight of organosilicon resin, and an organosilicon antifouling coating is prepared after substrate pretreatment, ultrasonic dispersion, curing and degassing, and spin coating curing. The coating of this invention achieves integrated antifouling and antibacterial properties, with an immediate bactericidal rate of 99% against Staphylococcus aureus and Pseudomonas aeruginosa, and an antibacterial rate of >90% after 60 days in a simulated marine environment. It effectively solves the problems of photocorrosion, short antifouling cycle, and poor mechanical properties of traditional photocatalytic materials, and is suitable for preventing biofouling on metal surfaces of marine facilities. The process is green and economical.

The application provides an earthworm antibacterial peptide derived from a popular cavity earthworm and application thereof in broad-spectrum antibacterial aspects. The amino acid sequence of the antibacterial peptide is shown in SEQ ID NO. 1 or SEQ ID NO. 2, and both have 13 amino acid residues and isoelectric points of 10.31 or 10.06. The application adopts a bioinformatics method, finds a new earthworm antibacterial peptide through a machine learning algorithm, optimizes the amino acid sequence according to the prediction result, and finally obtains an antibacterial peptide with better antibacterial effect. The obtained antibacterial peptide has good antibacterial activity on gram-negative bacteria represented by escherichia coli and gram-positive bacteria represented by staphylococcus aureus, and various drug-resistant bacterial variants of the two kinds of bacteria, and can be applied to the preparation of antibacterial products, including antibacterial drugs, additives and preservatives and the like.

The application discloses a kind of concrete anticorrosive coating with antibacterial and self-repairing and preparation method thereof, the anticorrosive coating includes the following weight parts of raw materials: 20-50 parts alicyclic epoxy resin, 100-150 parts vinyl resin, 30-50 parts multifunctional curing agent, 5-15 parts polyvinylamine resin, 10-25 parts silicone resin, 1-3 parts silane coupling agent, 0.5-2 parts initiator, 3-8 parts epoxy resin curing agent and 30-50 parts filler;The multifunctional curing agent includes the following weight parts of raw materials: 10-15 parts vinyl epoxy, 15-20 parts allyl thiocyanate and 5-15 parts allyl methyl disulfide.The concrete anticorrosive coating of the application contains multifunctional curing agent, which has thiocyanate group and disulfide group, the thiocyanate group can effectively inhibit the activity of escherichia coli and staphylococcus aureus, prevent escherichia coli and staphylococcus aureus from corroding the coating, and improve the service life of the anticorrosive coating;Disulfide group can undergo reversible rupture and recombination, with self-repairing ability.

The application discloses an antibacterial peptide derived from the skin of bullfrog and application thereof, and the amino acid sequence of the antibacterial peptide is GSVGPVGPR (as shown in SEQ ID NO:1), and the molecular weight is 824.4504 g / mol. The antibacterial peptide has obvious inhibiting effect on Staphylococcus aureus, and can be used for preparing a medicine for preventing or inhibiting Staphylococcus aureus infection. Meanwhile, the antibacterial peptide and vitamin A have a significant synergistic bactericidal effect on Staphylococcus aureus. The antibacterial peptide and vitamin A can significantly reduce the use concentration of single component, improve the antibacterial efficiency, overcome the bacterial drug resistance problem, and can be used for preparing antibacterial medicines, skin external preparation, medical dressings, feed additives and food preservatives.

This invention belongs to the field of biomedical materials technology, and relates to a piezoelectrically enhanced nanoenzyme composite material, its preparation method, and its antibacterial application. Cobalt-doped molybdenum disulfide is grown in situ on the surface of bismuth titanate nanosheets to obtain a BTO@Co-MoS2 composite material. This composite material exhibits a piezoelectric effect under pressure stimulation. Electrons, under the influence of the built-in electric field at the BTO@Co-MoS2 interface, promote the self-cycling of the molybdenum disulfide redox pair, thereby improving the catalytic activity of halogen-like peroxidases. Simultaneously, the piezoelectric catalysis of hydrogen peroxide generation alleviates the problem of insufficient endogenous hydrogen peroxide. In antibacterial applications, the piezoelectrically enhanced nanoenzyme composite material prepared by this invention achieves bactericidal rates of 98.39% and 99.95% against Staphylococcus aureus and Escherichia coli, respectively.

The application discloses an antibody-antibiotic conjugate and application thereof, and a structural formula of the antibody-antibiotic conjugate is shown as formula (I) and is composed of a payload antibiotic, a monoclonal antibody targeting Kdo monosaccharide and an enzyme-sensitive linker; the antibody-antibiotic conjugate has strong affinity to Kdo antigen and can be combined with Staphylococcus aureus of different serotypes. Enzymatic treatment of the antibody-antibiotic conjugate can release free antibiotics to improve the bactericidal efficiency on Staphylococcus aureus; the antibody-antibiotic conjugate can effectively kill intracellular bacteria which are difficult to be affected by traditional small-molecule antibiotics through the mediation of the antibody; in the application of a Staphylococcus aureus-induced mouse pneumonia and nephritis model, the antibody-antibiotic conjugate can effectively remove bacteria in the lung and kidney, and the therapeutic effect is better than that of vancomycin. Therefore, the antibody-antibiotic conjugate has potential application in the development of Staphylococcus aureus infection drugs.

The application discloses application of BMS-833923 and derivatives thereof and a medicine, and belongs to the technical field of antibiotics. The BMS-833923 and the derivatives thereof can be used as colistin adjuvants to jointly inhibit or eliminate gram-negative bacteria, and can also independently inhibit or eliminate gram-positive bacteria. The gram-negative bacteria can include at least one of Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, pan-drug-resistant strains BAA-1800, BAA-1794 and BAA-1792, and the gram-positive bacteria can include at least one of Staphylococcus aureus and Bacillus subtilis. By taking the BMS-833923 and the derivatives thereof as adjuvants of colistin, the therapeutic index of the colistin can be effectively expanded, and lower, non-toxic doses of the colistin can be used in clinical treatment of effective treatment of drug-resistant bacterial infections.