410 results about "Brown spot" patented technology

The invention relates to a synthesis and fungicidal activity of an aryl ether based triazole compound which comprises a structural formula I, the general expression is shown in the figure, in which R represents a hydrogen atom, an alkyl, a methoxyl, a nitro or a halogen. The synthesis and fungicidal activity of an aryl ether based triazole compound has the advantages of strong specificity inhibitory activity to peanut brown spot pathogen at 50ppm to 200ppm and ability to be used for preparing agricultural fungicides.

A comprehensive, single agent cosmetic cream or lotion containing a high number of ingredients that target anti-aging in a defined manner. The cream or lotion contains a high number and variety of active substances that demonstrate excellent safety and efficacy in all of the various defined categories of skin aging, including but not limited to wrinkles, abnormal pigment or brown spots due to aging of the skin and an unexpectedly high efficacy in the reduction of redness and acne and rosacea blemishes.

The invention discloses a streptomyces microflavus. The streptomyces microflavus is the streptomyces microflavus NMG2-4-8, and the preservation number thereof is CGMCG No.3442. The streptomyces microflavus is separated and screened from alfalfa healthy tree rhizosphere soil for the first time in the invention, and experiments prove that the streptomyces microflavus has remarkable antagonistic action for 18 pathogenic fungi and 9 bacteria and broad antibacterial spectrum. The streptomyces microflavus has strong inhabiting action for crop pathogenic bacteria, such as alfalfa downy mildew and the like, shows a good disease prevention effect for the alfalfa downy mildew, powdery mildew, brown spots, stemphylium leaf spot disease, root rot disease and the like, and indicates that the bacterial strain has broad application prospect in the biological prevention and cure fields of alfalfa diseases.

The invention discloses a selective breeding method of a high-resistance tobacco variety and belongs to the technical field of resistive breeding of tobacco. According to the selective breeding method, the tobacco high-yield varieties Yunyan 85, Yunyan 87 and Zhongyan 100 or K326 are taken as recurrent parents, and the cold-resistant variety Honghua Dajinyuan, the drought-resistant variety Zhongyan 14, the brown spot and blackleg resistant variety Zhongyan 86, the root-knot nematode-resistant variety TI1706, the common mosaic virus-resistant variety Taiyan No.7 and the tobacco bacterial wilt-resistant variety D101 as resistant resource parents, and the seven major drought, cold and disease resistant resource genes of the tobacco are pyramided and inserted into the good-quality high-yield tobacco variety with the same genetic background by use of a synchronous backcross and convergent cross method, so that a new high-resistance, high-yield and good-quality tobacco variety, which is highly resistant to 7 drought, cold and disease damage factors and highly consistent with the original recurrent parents in other agricultural and yield traits, is obtained.

The invention belongs to the field of biotechnology and particularly relates to Bacillus subtilis and application thereof. The Bacillus subtilis is Bacillus subtilis HS5B5. The strain is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6088. The Bacillus subtilis HS5B5 provided by the invention has a very strong restraining effect on the pathogen Cladosporiwm paeoniae of brown spot of peony, and can effectively prevent and cure brown spot of peony. The Bacillus subtilis HS5B5 is harmless to the ecological environment and cannot cause the resistance of the pathogen. The Bacillus subtilis HS5B5 is cultured under simple conditions, is easy to preserve, facilitates industrial production and has a favorable development and application prospect.

The invention provides Bacillus siamensis and application thereof, and belongs to the technical field of microbial prevention and control. Research shows that Bacillus siamensis LZ88 provided by the invention has very good effects on the biological control of plant diseases such as tobacco brown spot, tobacco black shank, wheat scab, grape anthracnose, cucumber fusarium wilt, peanut fusarium and the like, the induction of the resistance to tobacco plant diseases and the like, and provides a new strain resource for biological prevention and control of main diseases of tobacco, such as brown spot, and the like, thereby having good practical application value.

The invention discloses a bacillus firmus strain. The bacillus firmus strain is bacillus firmus Bf-02 CGMCC No.3129, and can prevent and control a plurality of plant diseases, such as the anthracnose of walnuts, the gray mold of cucumber, wheat sharp eyespots, the ring rot of apples, tobacco brown spots, corn southern leaf blight, Dongzao jujube black patch, cotton fusarium wilt, cotton red rot, wheat scab and the like. Tests prove that the zymotic fluid of the bacillus firmus Bf-02 CGMCC No.3129 has remarkable effect of preventing and controlling vegetables, particularly the gray mold of the cucumber with the healthy growth of the vegetables and without hazards, and also remarkable growth promotion and yield increasing effects on the cucumber with the growth rate of 29.95 percent after one-time application.

The invention provides bacillus amyloliquefaciens cd5bc. The strain is classified and named as Bacillus amyloliquefaciens cd5bc and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2016232, and the preservation time is April 28, 2016. The invention further provides application of the strain in the aspect of preventing and controlling crop diseases and a biocontrol preparation prepared from the strain. The strain has a very significant preventing and controlling effect on red pepper phytophthora blight, the biocontrol effect reaches up to 100% after inoculation is conducted for 15 days, and the biocontrol effect reaches up to 82.36% after inoculation is conducted for 60 days. The bacillus amyloliquefaciens cd5bc has excellent disease-resistant effects on multiple crop diseases, the bacteriostasis rates of the strain on pathogenic bacteria of red pepper sclerotinia blight, bitter gourd wilt, cucumber wilt, cucumber scabs, rice blast, a rice sheath blight disease, broad bean chocolate spots and broad bean brown spots all reach up to 70% or above, and the bacteriostasis rate of the strain on the broad bean chocolate spots and broad bean brown spots reaches up to 100%.

Citrus canker and brown spot are common diseases of citrus. At present, few drugs are available to prevent and control the two diseases and have certain defects. Amino groups at the 7th positions of fluoroquinolone drugs are linked with an active fragment by means of a connecting structure so as to obtain compounds shown in a formula I or a formula II. Experiments prove that the compounds providedby the invention have effects of preventing and controlling the citrus canker and the brown spot and have very good application prospect.

The invention discloses a bamboo dice online detection method based on vision. The method includes the following steps that 1, bamboo dices to be detected move through a second conveyor belt, and an image triggering and collecting module carries out photographing to collect images and numbers the images; 2, the images are transmitted to a PC for white balance processing so that images according with human vision can be obtained, then whether defects exist in the images of the bamboo dices to be detected or not is judged according to a bamboo dice defect detection algorithm, and defective bamboo dices to be detected are removed, wherein the detection process specifically includes the steps of outline defect detection, white spot detection and brown spot detection. According to the method, the bamboo dices can be automatically conveyed and automatically turned over, image information of the bamboo dices to be detected is collected through the image triggering and collecting module, the appearance defect, the white spot defect and the brown spot defect of the bamboo dices are detected and judged on line through the PC according to the defined bamboo dice defect detection algorithm, and the defective bamboo dices are removed; the manual picking cost is significantly reduced, the bamboo dice detection efficiency is improved, the product percent of pass is increased, input is small, operation is convenient, and the method has broad market prospects.

The invention discloses a method for detecting the 4-methyl imidazole in food quickly belonging to food additives detection range. The sample containing caramel pigment is extracted in solid phase through solid phase extraction pile (XTR is more than 500mg), after the sample passes through the pile, it is rinsed with organic solvent, the purged liquid is condensed, uses micro sample applicator to spot sample directly on the chromatography material polyamide film, and it is developed in the developing agent with the same proportion, observes it with naked eye after it is color developed with iodine steam, it observes the brown spots with the rf values the same to the standard sample in rf range between 0.5 and 0.7, then considers that the measured material has 4-methyl imidazole. It can detect if the measured sample is natural or is confected manually, especially proper to detect the sample such as sauce, vinegar and cola containing caramel pigment.

The invention discloses a bactericidal composition containing trifloxystrobin and tebuconazole, which contains the effective active components trifloxystrobin and tebuconazole. The weight ratio of the trifloxystrobin to the tebuconazole is 60:1-1:60. The bactericide composition contains at least one surfactant, and thus, the active components can be easily dispersed in water. The bactericidal composition has the activities of protection, treatment and removal, and can be used on multiple crops, such as wheat, rice, vegetables, fruit trees and the like, for preventing and treating leaf blight, brown leaf rust, glume blight, brown spot, powdery mildew and the like.

The invention provides acid-resistant pseudomonas protegens CLP-6 and application thereof. According to the acid-resistant pseudomonas protegens CLP-6 and the application thereof, the bacterial strainof the pseudomonas protegens CLP-6 is preserved in China General Microbiological Culture Collection Center on October 27th, 2016, and the biological preservation number of the pseudomonas protegens CLP-6 is CGMCC No.13205; the bacterial strain of the pseudomonas protegens CLP-6 has acid resistance, is good in disease prevention and plant growth promotion effects and high in stress resistance, hasthe antagonistic activity of pathogenic bacteria, is wide in soil acidity tolerance range and wide in antibacterial spectrum, and has application and development potential in prevention and treatmentof bacterial wilt, black shanks and brown spots; and meanwhile, under the general background that the acidification conditions in China is increasingly severe, the research on the bacterial strain ofthe pseudomonas protegens CLP-6 has reference significance in prevention and treatment of plant diseases under various soil qualities, a microbial resource is provided for green prevention and control over the bacterial wilt, the black shanks and the brown spots in acid soil, and secondary metabolites of microorganisms are excavated, so that an important foundation can be laid for the biocontrolefficacy on the bacterial wilt, the black shanks and the brown spots in the acid soil.

The invention provides an agricultural fungicide WYLZ-1349 taking azalomycin F3, azalomycin F4 and a nyphimycin composition as effective constituents, as well as preparation method and application thereof. The preparation method of the agricultural fungicide WYLZ-1349 comprises the following steps of: firstly culturing Streptomyces malaysiensis ECO 00002 with the preservation number of CGMCCNo. 1680; then separately extracting a fermentation culture; breaking the wall of mycelium with acetone or methanol and mixing with a fermentation supernate; and absorbing by macroporous absorption resin for separation; eluting by the acetone or the methanol; concentrating or further purifying an eluent so as to obtain the agricultural fungicide WYLZ-1349 or the azalomycin F3, the azalomycin F4 and nyphimycin; and then adding a carrier or an auxiliary agent so as to obtain a finished product. Test results show that the agricultural fungicide WYLZ-1349 has good effect especially in preventing and controlling rice blast, tobacco brown spot, tomato gray mold and pepper anthracnose and has small using amount, low using cost for peasants, wide usable range, and the like compared with the traditional medicament.

Disclosed is the agricultural pesticide containing camptothecine or camptothecine derivatives and the use thereof, wherein the pesticide comprises camptothecine or its derivatives 0.02-2.0%, emulsifying agent 1.0-20%,penetrating agent 0.5-10%, and balancing organic solvent. The pesticide can be used for controlling agronomic crop downy mildew, leaf streak, pythium blight, head blight, root and stem rot, rhizoctonia rot, damping-off, wilt,bitter rot, powdery mildew, black shank, brown spot, leaf scab, ring rot, black pox, gray mold,phytophthora rot, ulcer disease, black spot, bacterial wilt, angular leaf spot, leaf streak and leaf scar.

The invention discloses a biocontrol bacterium and azoxystrobin composition belonging to the technical field of bactericides. The biocontrol bacterium and azoxystrobin composition contains active components including bacillus subtilis and azoxystrobin. The biocontrol bacterium and azoxystrobin composition disclosed by the invention can be used for remarkably reducing the medication cost while effectively preventing and controlling various diseases such as rice sheath blight disease, rice false smut, rice blast, ear rot, seedling-stage damping-off, tomato late blight, gray mold, wheat powdery mildew, sheath blight, cotton rhizoctoniosis, foxysporum, verticillium dahliae, soybean root rot, corn sheath blight, cucumber gray mold, blight, tobacco brown spot, bacterial wilt, various field crop and garden crop root rots and damping off, so that the medication cost can be reduced, the preventing and controlling ranges can be widened, the environment pollution can be reduced, and the phytotoxicity and pesticide resistance risks can be reduced.

The invention relates to a phytoplankton culture method, belonging to the field of fresh water plant culture. The method for culturing high-density fresh water attached diatom is characterized by comprising the following steps: preparation of feed rotten liquid: by using a common fresh water fish compound feed, adding 3-5 times by volume of tap water, and carrying out rotting fermentation until the odor becomes weak; adding tap water and the feed rotten liquid into a glass container, wherein larger particles are filtered out before adding the feed rotten liquid; putting the glass container in a glass greenhouse or in an outdoor place under solar radiation (keeping out rain in rainy days), and ensuring the lighting condition to be solar radiation intensity of winter and spring; and heating the water body in the glass container to keep the temperature of the water body at 20-30 DEG C while aerating the water body for 10-14 days, wherein red brown spots, which are diatom, can grow on the wall of the glass container.

The invention discloses a high-yield cultivation method of interplanting of pinellia ternata in apple orchards, belonging to the technical field of interplanting cultivation. The high-yield cultivation method of interplanting of pinellia ternata in apple orchards has the following beneficial effects: apple trees are utilized to shield pinellia ternate from intense light such that pinellia ternate is protected against sprout tumble or death due to exposure under intense light, thereby decreasing production cost; gaps among apple trees are fully utilized so that land resources are saved; meanwhile, fertilizers and water resources in the process of growing pinellia ternate are utilized; without additional fertilizers and water nutrients, the nutrition demand during the growth of apple trees is met; as a result, yield of apples is increased and the incidence rate of brown spots and rotten diseases during the process of planting apples is reduced; yield of pinellia ternate is greatly improved; and the content of needle-like calcium oxalate crystal with stimulating components in pinellia ternata is also decreased, thereby ensuring medical security of pinellia ternate to the large extent.

The invention discloses a biocontrol bacterium and pyraclostrobin composition belonging to the technical field of bactericides. The biocontrol bacterium and pyraclostrobin composition contains active components including bacillus subtilis and pyraclostrobin. The biocontrol bacterium and pyraclostrobin composition disclosed by the invention can be used for remarkably reducing the medication cost while effectively preventing and controlling various diseases such as rice sheath blight disease, rice false smut, rice blast, ear rot, seedling-stage damping-off, tomato late blight, gray mold, wheat powdery mildew, sheath blight, cotton rhizoctoniosis, foxysporum, verticillium dahliae, soybean root rot, corn sheath blight, cucumber gray mold, blight, tobacco brown spot, bacterial wilt, various field crop and garden crop root rots and damping off, so that the medication cost can be reduced, the preventing and controlling ranges can be widened, the environment pollution can be reduced, and the phytotoxicity and pesticide resistance risks can be reduced.

The invention discloses a bactericidal composition containing pyraclostrobin and prothioconazole and application thereof. The bactericidal composition uses the pyraclostrobin and prothioconazole as main effective components, wherein the weight ratio of the pyraclostrobin to the prothioconazole is (1-70):(1-50). The bactericidal composition can be used for preventing and treating diseases on cereals, melons and fruits, or vegetable crops, has higher synergic effect, overcomes and postpones the drug resistance of pathogenic bacteria, has the advantages of high bactericidal speed and long duration time, lowers the use cost, and has obviously better preventing and treating effects than a single agent. The bactericidal composition can be used for preventing and treating fungus diseases on the crops, especially can be used for preventing and treating leaf blight, rust disease, powdery mildew, downy mildew, epidemic disease, anthracnose, scab, brown spot, seedling blight, gray mold, banana freckle, penicilliosis and other fungus diseases, and has better effect than a single agent.

The invention discloses a method for detecting the titre of live viruses, which comprises the following steps: firstly, infecting diploid cells or other adherent cells with dilute viruses to be detected, culturing and fixing the infected cells; secondly, adding virus antiserum (first antibody) into the fixed cells, culturing and washing, adding a second antibody marked by horseradish peroxidase (HRP), culturing and washing, adding coloring solution for color development and producing red or brown spots, or adding virus antiserum marked by the HRP into the fixed cells, culturing the virus antiserum and washing, adding the coloring solution for color development and producing red or brown spots; thirdly, calculating the virus titre according to the quantity of the red or brown spots and the dilution multiple, or calculating the 1gTCID50 value of the viruses according to the dilution degree and the hole numbers of the spots. The detection method can be applicable to the titre detection of various live viruses, can shorten the detection time of the titre of the viruses due to fast detection speed, has good specificity as the immune spots are caused by special immune reaction, low detection cost as well as simple and convenient operation and does not need special instruments of optical or fluorescent microscopes, and the like or other reagents.

The invention discloses an alternaria alternate spore suspension liquid and an application thereof. The alternaria alternate spore suspension liquid is prepared through germ separation, germ cultivation, spore production through induction, and preparation of spore suspension liquid. The alternaria alternate spore suspension liquid is used in measurement of tobacco brown spot, or in screening of drug resisting the tobacco brown spot, biocontrol bacterium or natural products, or in research of soil ecology of the alternaria alternate and germ infect circulation. The alternaria alternate spore suspension liquid adopts a multipoint inoculation method, has fast cultivation speed and short cultivation cycle; the alternaria alternate is promoted to generate large quantity of spores rapidly through the comprehensive action of methods such as low temperature high humidity stimulation, gradient cooling, dark induction and the like, and the quantity of the spore generated is 50-100 times that of conventional spore generation quantity. The alternaria alternate spore suspension liquid is light in contamination, the time for cultivating hypha and generating spore through induction is relatively fixed and concentrated, spore generation and growing are synchronous, and the spores prepared in the same batch and different batches are small in quality difference and more stable in quality.