71 results about "Brown spot" patented technology

The invention relates to the technical field of computer vision, in particular to a jujube tree disease and insect pest recognition method and system combined with a vision technology, and the method comprises the steps: extracting closed edge contours of a disease leaf gray image, and obtaining a contour skeleton of each closed edge contour; based on the graphic features of each contour skeleton, obtaining a to-be-recognized region, analyzing the distribution of gradient amplitudes of the skeleton contour edge and inner pixel points and the gradient amplitude distribution of the skeleton contour outer pixel points, and obtaining an edge transition value of each to-be-recognized region; based on the overall distribution characteristics of the gradient amplitudes of all edge pixel points of the skeleton contour of each to-be-identified area, obtaining an edge energy value of each to-be-identified area; and determining an edge distinguishing value of each to-be-identified area, and obtaining a disease identification result of each to-be-identified area. The invention aims to improve the identification capability of the jujube brown spot and the jujube gray leaf spot and improve the identification precision of disease detection.

The invention relates to a cotton leaf disease identification method and system based on multilayer context feature screening, and belongs to the field of computer vision. According to the ConvNeXt-based cotton leaf disease identification method, a cotton leaf disease data set is constructed, and a system capable of identifying cotton physalospora piricola, brown blotch, angular leaf spot, verticillium wilt, fusarium wilt, anthracnose and powdery mildew and returning early warning information and prevention and treatment measures is established. According to the method provided by the invention, the ConvNeXt network is used as a backbone network, a feature enhancement module is added, the contrast among different regional features of the cotton leaves is improved, and on the basis, a feature screening attention mechanism of feature screening guided by multilayer context information is introduced, so that more effective feature screening is realized, and experiments show that the feature screening attention mechanism is more accurate. Compared with the recognition accuracy of the original ConvNeXt network, the recognition accuracy of the network model provided by the invention is improved by 4.1%.

The invention relates to application of receptor-like kinase MdRLK2 in comprehensive resistance breeding of apples. After the receptor-like kinase MdRLK2 is instantaneously overexpressed in the apple tissue culture seedling Gal-3 and the apple Gala branch, it is found that the improvement of the expression quantity of the receptor-like kinase MdRLK2 effectively enhances the capability of resisting alternaria leaf spot, anthrax leaf blight, brown spot, rot disease and ring spot of apples; therefore, a molecular means for improving the comprehensive disease resistance of the apples is developed by overexpressing the apple receptor-like kinase MdRLK2, and meanwhile, a theoretical basis is provided for molecular breeding of the apples.

The invention discloses a detection kit and a detection method for visually detecting citrus brown spot bacteria based on RPA-CRISPR / Cas12a. According to the scheme, an A.alternana visual rapid detection system based on an RPA-CRISPR / Cas12a technology is provided, the system can be carried out under the constant temperature condition of 37 DEG C, the detection lower limit is 10 <-3 > ng / mu L, the reaction time is only 40 min, the detection result can be visually reflected by means of a nucleic acid test strip, and the detection result can be compared with the prior art. The method has the advantages of high specificity, high sensitivity, short detection time, visualization and the like, is not limited by materials, sites and instruments, can detect target pathogenic bacteria from complex samples of various tissues of plants, and is simple and easy to use for farmer households and grassroots plant protection workers.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The application relates to the technical field of computer vision, in particular to a jujube tree disease and pest identification method and system combined with visual technology, which comprises the following steps: extracting a closed edge contour of a disease leaf gray image, and obtaining a contour skeleton of each closed edge contour; based on the graphic features of each contour skeleton, obtaining a to-be-identified region, analyzing the gradient amplitude distribution of the skeleton contour edge and the inside pixel points, the gradient amplitude distribution of the skeleton contour outside pixel points, and obtaining an edge transition value of each to-be-identified region; based on the overall distribution features of the gradient amplitude of all edge pixel points of the skeleton contour of each to-be-identified region, obtaining an edge energy value of each to-be-identified region; determining an edge distinguishing value of each to-be-identified region, and obtaining a disease identification result of each to-be-identified region. The application aims to improve the identification ability of jujube brown spot disease and jujube gray spot disease and improve the identification precision of disease detection.

The invention discloses an intelligent monitoring, prevention and control system for tobacco brown spot, and relates to the technical field of agricultural disease monitoring and intelligent prevention and control. A lower canopy monitoring array, elevation thermal infrared and red edge near infrared imaging and spore sampling (fluorescent quantitative PCR / loop-mediated isothermal amplification) are used as inputs; fog drop deposition D, leaf surface water film W and spore concentration S are predicted through multi-modal alignment and a physical field generator; carrying out long sequence risk assessment by inputting a correlation state space model and superposing a liquid state self-correlation kernel, and carrying out long sequence risk assessment by using concentric wheel patterns and narrow yellow halo (rlt; 0.15) + near-infrared / short-wave infrared black mould layer triple discrimination and checking, and a prescription is formed by combining double-pulse triggering that leaf surface wetting is larger than or equal to 8 h or a spore index reaches a threshold. The prescription comprises night microclimate intervention, variable spraying and leaf thinning control; resistance rotation compliance is realized through prescription uplink pre-check and electronic control unit unlocking; based on row belt / Thiessen polygon scheduling, 36 h coverage is larger than or equal to 95%, the dosage and residues are reduced, and the hit rate and timeliness are improved.

The utility model belongs to the technical field of agricultural product preservation, and particularly relates to a lotus root anti-brown spot preservation container which comprises a base, a splicing bin and a bin cover, a filling bin filled with natural antioxidants and microbial inhibitors is arranged in the splicing bin, and the lotus root anti-brown spot preservation container can inhibit the activity of phenol oxidase, prevent brown spots, inhibit the growth of mould and bacteria and delay lotus root decay; a sponge mat is arranged at the bottom of the container to absorb excessive moisture, keep dry and further prevent mould breeding; a microporous membrane is arranged between the splicing bin and the bin cover, gas exchange is allowed, microorganisms are prevented from entering, the concentration of oxygen and the concentration of carbon dioxide are controlled, and the preservation effect is enhanced; the quick-release lock catch structural design enables the container to be mounted and dismounted more conveniently and quickly, the air holes are formed in the partition pieces, air circulation is ensured, a suitable storage environment is provided, and the overall design is efficient, environmentally friendly and easy and convenient to operate.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof in plant disease control. A bacillus velezensis strain is separated and screened from rhizosphere soil of a wolfberry plant in a Nomuliflood farm in Qinghai province, the strain is bacillus velezensis G3 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 36136. The bacterial strain has a better antibacterial effect on pathogenic bacteria fusarium oxysporum of Chinese wolfberry root rot, also has an inhibiting effect on pathogenic bacteria of Chinese wolfberry verticillium wilt, botrytis cinerea, sclerotinia rot of colza, alternaria alternata, apple anthracnose, apple rot, pear wilt and wheat scab, and has broad-spectrum antibacterial activity; wide application prospects are realized in plant disease prevention and treatment.

The invention discloses a tea group fine manipulation degree identification method based on MobileNet V3, and the method comprises the steps: obtaining a tea group image, obtaining a tea fine manipulation data set through data enhancement, and dividing the tea fine manipulation data set into a training set, a verification set and a test set; preprocessing the group image to segment brown spots and red edging features in a fine manipulation stage; the method comprises the following steps of: improving a MobileNet V3-large model: adding an adaptive residual branch in a Bcheck module, and using a GELU activation function and an improved CA attention mechanism; the improved MobileNet V3-large model is trained, and a final improved MobileNet V3-large model is obtained through the training of the improved MobileNet V3-large model and the training of the improved MobileNet V3-large model; and predicting and outputting identification results of the color, the form, the brown spots and the red edges of the tea leaves by utilizing the final improved MobileNet V3-large model. According to the invention, intelligent judgment of the tieguanyin green-making degree is realized.

The invention discloses a method for efficiently preventing and controlling peach pulp brown spot disease, which comprises the following steps of: brushing specific liquid medicine on the surfaces of branches and trunks of peach trees, manufacturing a fence around the trunks by using a plastic flat net, filling special lime soil in gaps between the fence and the trunks, and pouring specific fertilizer liquid; the positions, above the fence, of the trunk, the main branches and the side branches are bound through non-woven fabric belts soaked with liquid medicine; afterwards, the lime soil filled in the fence is regularly watered with fertilizer liquid, and the fertilizer liquid is sprayed on the cerclaged non-woven fabric belt. The method disclosed by the invention is simple and easy to operate and efficient and safe in action, and the prevention effect on the peach pulp brown spot disease can reach 80% or above.

The utility model belongs to the technical field of agricultural machinery, and particularly relates to an anti-brown-spot layered pruning support suitable for canopies of tangelo trees. Comprising first connecting rods, second connecting rods, first connectors, second connectors and telescopic stand columns, the multiple first connecting rods and the multiple first connectors are connected to form a first closed loop, the multiple second connecting rods and the multiple second connectors are connected to form a second closed loop, and the adjacent first connectors are connected with the second connectors. Layered pruning management is achieved, the light transmittance is improved, humidity accumulation is reduced, the support can be rapidly assembled, the device adapts to different tree ages and tree forms, the manual climbing risk is reduced, operation is easy and convenient, and practicability is high.

This invention belongs to the field of biodetection technology, specifically relating to a primer set, kit, and application for detecting *Alternaria solani* brown spot disease. The base sequences of the primer set are shown in SEQ ID NO.1~SEQ ID NO.5. Based on the primer set, this invention utilizes loop-mediated isothermal amplification technology for the rapid detection of *Alternaria solani*, accurately detecting the fungus from the complex pathogenic environment in diseased plant tissues. The rapid detection system described in this invention exhibits high specificity and sensitivity, with a detection limit of 0.246 fg / μL for *Alternaria solani* DNA. Using the rapid detection system provided by this invention, *Alternaria solani* can be detected rapidly, conveniently, accurately, and sensitively from field samples within 45 minutes, which is simpler and more efficient than traditional symptom identification methods and ordinary molecular detection methods.

This invention relates to the field of microbial technology, specifically to a strain of *Bacillus laterosporus* and its applications. The *Bacillus laterosporus* provided by this invention (… Brevibacillus laterosporus HNBR05-1 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 25886. *Bacillus laterosporus* HNBR05-1 exhibits highly effective inhibition of plant pathogens, demonstrating dual efficacy against plant diseases such as brown spot and bacterial leaf spot, with high control efficacy. It shows promising application prospects in plant disease control and lays the foundation for the development of highly effective biocontrol agents.

The invention belongs to the field of pesticide compositions, and particularly relates to a composition for resisting tobacco brown spot. The active ingredients of the composition are soybean saponin and a composition A, and the composition A is composed of cuaminosulfate and hymexazol according to the mass ratio of 1: 3. The preferable mass ratio of the soybean saponin to the composition A is 0.1: 0.01. Indoor toxicity determination and field test results show that the ternary composition has a remarkable synergistic effect on tobacco alternaria alternata pathogenic bacteria, and the field control effect reaches 93.07% at most and is far superior to the effect of each single agent. According to the invention, by introducing the plant source component soyasaponin, the use amount and the residual risk of chemical pesticides are remarkably reduced while the excellent control effect is ensured, and a brand new solution is provided for green and efficient prevention and control of the tobacco brown spot.

The invention discloses a multiplex PCR (polymerase chain reaction) method for quickly detecting pathogenic bacteria of tobacco brown spot, which is used for synchronously identifying two main pathogenic bacteria causing tobacco brown spot, namely alternaria tenuissima and alternaria alternata, and comprises the following steps: S1, extracting genome DNA (deoxyribonucleic acid): extracting genome DNA of standard strains of alternaria tenuissima and alternaria alternata as a detection template; the multiple PCR system has the beneficial effects that through system verification, it is confirmed that all primers only generate expected amplification products for target pathogenic bacteria, no cross amplification reaction exists between alternaria alternata and alternaria tenuissima, and it is indicated that the multiple PCR system has high interspecific specificity. The established detection method is high in stability, good in repeatability and suitable for standardized operation under different laboratory conditions. Compared with a traditional morphological identification or single gene sequencing method, the multiplex PCR technology has the advantages that the detection period is remarkably shortened, the operation is simple and convenient, time and labor are saved, the cost is low and the like. The method is suitable for rapid identification of standard strains.

The application discloses a Trichoderma koningiopsis and application of the Trichoderma koningiopsis as a biocontrol agent in prevention and treatment of plant diseases. Trichoderma koningiopsis The Trichoderma koningiopsis GS95 screened by the application is preserved in the China Center for Type Culture Collection (CCTCC) and has a preservation registration number of CCTCC NO: M 20252046. The Trichoderma koningiopsis GS95 screened by the application has a strong inhibiting effect on a plurality of filamentous fungal pathogenic bacteria, especially in prevention and treatment of blueberry top bud brown spot caused by new outbreak of Cladosporium oxysporum on blueberries, and provides an effective biocontrol agent for the disease caused by the newly discovered pathogenic bacterium Cladosporium oxysporum.

The application discloses a Trichoderma spirale and application of the Trichoderma spirale as a biocontrol agent in prevention and treatment of plant diseases. Trichoderma spirale The Trichoderma spirale GS98 screened by the application is preserved in the China Center for Type Culture Collection, and the preservation registration number is CCTCC NO: M 20252045. The Trichoderma spirale GS98 screened by the application has a strong inhibiting effect on a plurality of filamentous fungal pathogenic bacteria, especially in prevention and treatment of blueberry top bud brown spot caused by a newly-broken-out Cladosporium oxysporum on blueberries, and provides an effective biocontrol agent for a disease caused by the newly-discovered pathogenic bacterium Cladosporium oxysporum.

The invention relates to a three-way hybrid grouper new germplasm cultivation method, and belongs to the technical field of grouper genetic breeding. According to the method, epinephelus moara and golden tiger hybridized fries (female epinephelus fuscoguttatus * male epinephelus coioides) are used as breeding materials, three-way hybridization is carried out in a matching mode of female epinephelus moara * male epinephelus coioides, and breeding of three epinephelus genetic species and fry with excellent characteristics is achieved. The method comprises the steps of parent selection, parent fish reproduction regulation and control, parent fish spawning induction, gamete collection, artificial insemination, incubation and fry rearing. According to the method, on the basis of reproductive regulation and crossbreeding technologies, time and space reproductive isolation of the groupers is broken through, and the new grouper germplasm which is high in survival rate, low in malformation rate and high in growth speed is cultivated.

The present invention discloses a method for simultaneously identifying anthracnose, powdery mildew, and brown spot diseases of honeysuckle based on real-time PCR. The method detects the three pathogens of honeysuckle, powdery mildew, and brown spot diseases, selects the ITS gene as a target gene, designs primers and TaqMan fluorescent probes, and performs fluorescent PCR amplification to rapidly and accurately identify the pathogens of anthracnose, powdery mildew, and brown spot diseases of honeysuckle. The method is used to rapidly and accurately identify the pathogens of anthracnose, powdery mildew, and brown spot diseases of honeysuckle, lays an etiological foundation for the prevention and treatment of the three diseases of honeysuckle, and is of great significance for ensuring the stable growth of honeysuckle quality and yield.

The present application relates to the technical field of pesticide compounding, and discloses a fungicidal composition containing allylazole and thiazolamide, wherein the weight ratio of the allylazole to the thiazolamide is 1:50-50:1. The pesticide composition is mainly used for preventing and treating rice sheath blight, tobacco brown spot and tomato early blight. Since the two components have different action mechanisms, the composition has obvious synergistic effect, can effectively reduce the use amount of pesticides, and has good economic and social benefits in popularization and application, and helps to realize the reduction and synergism of pesticides and meet the friendly trend of national green environmental protection.

The invention relates to a pear NAC transcription factor PpyNAC381 and application thereof in regulation and control of fruit rust formation. The DNA sequence of the pear NAC transcription factor PpyNAC381 is as shown in SEQ ID NO: 1. According to the invention, the expression mode of the gene in different treatment of peel of the Cuiguan pear is analyzed, and the consistency of the expression level of the gene and the phenotype of fruit rust is determined; the effect of the PpyNAC381 gene in pear fruit rust formation is verified by utilizing a transient overexpression technology, compared with control fruit peel, lignin and corpus of pear fruit peel overexpressing the PpyNAC381 are obviously accumulated, brown spots appear on the fruit peel, and the pear fruit rust is formed. The result of the invention is beneficial to clarification of the effect of the pear NAC transcription factor PpyNAC381 in regulation and control of the formation of the pear fruit rust from a molecular mechanism, provides a theoretical basis and gene resources for pear molecular breeding, and has a positive guiding effect on cultivation of a new rust-free / less-rust pear variety.

The invention relates to the technical field of detection kits, in particular to a chromogenic reaction detection method of a portable rapid borax detection kit.The method comprises the steps that 2 g of a sample is weighed and cut into pieces, 5 mL of distilled water is added, oscillation is carried out for multiple times, standing is carried out for 5 min, then supernate is taken as sample to-be-detected liquid, a piece of borax test paper is taken, sample soaking liquid is dropped to the middle of the test paper, and the sample to-be-detected liquid is obtained; then dropping a drop of curcumin solution at the same position, if reddish brown spots appear on the test paper, dropping a drop of hydrochloric acid on the spots, and if the test paper becomes green black or bluish violet, judging that the sample is doped with borax; the curcumin solution is used as a color developing agent, the curcumin solution and boric acid generate a specific reddish brown complex under the acidic condition, hydrochloric acid is used for reinforcing the color developing reaction, and the temperature-controlled electric hair drier is used for accelerating the drying of the test paper, so that the detection time is shortened to 5-10 minutes; therefore, the detection sensitivity is effectively improved, the anti-interference capability is enhanced, and the operation convenience and the application universality are greatly improved.

The utility model relates to the sugar beet planting technical field discloses a kind of sugar beet brown spot field monitoring devices, including support pile, support pile bottom is equipped with support base plate, and multiple groups of positioning insertion rod are passed through on support base plate, support pile top is equipped with mounting plate, and multiple groups of camera are installed on mounting plate upper side, and rain shed is equipped above camera;Multiple groups of environmental monitoring devices are installed on the outside of support pile, and environmental monitoring device includes multiple groups of humidity monitor and temperature monitor, and multiple groups of humidity monitor and temperature monitor are fixedly installed on the outside of positioning frame body, and positioning frame body is adjusted in height on the outside of support pile.The utility model has the advantages compared with prior art in that: environmental monitoring device can be adjusted in height on the outside of support pile with sugar beet growth state, monitor sugar beet on leaf upper side and rhizome, monitor sugar beet leaf growth state by camera, and can comprehensively monitor sugar beet growth state.

The invention relates to the field of crop breeding, in particular to a rice brown spot detecting and counting method, which has the technical scheme that by improving a YOLOv8 model, introducing a C2f-EFA module, an ASFPN module, a WSIoU loss function and a TADHead detection head and optimizing model performance, efficient and accurate rice brown spot detecting and counting are realized, the accuracy and efficiency of brown spot disease detecting and counting are improved, and the rice brown spot disease detecting and counting method is suitable for large-scale popularization and application. Technical support is provided for rice disease analysis; wherein the C2f-EFA module enhances the feature expression ability of the disease spots and the disease leaves of the brown spot, the parameter quantity and the calculation complexity are remarkably reduced, and the method is suitable for a brown spot detection scene with limited resources; the ASFPN module effectively improves the detection capability of the model on the brown spot disease spots and diseased leaves, and is suitable for processing scenes with large scale difference, complex background and small disease spots; the TADHead detection head enhances the generalization ability of a brown spot detection model, enhances the detection precision of brown spot disease spots and diseased leaves, and ensures robustness under the condition of complex background or low contrast; the WSIoU loss function obviously improves the positioning accuracy of the disease spots and the disease leaves.

The present application discloses a strain of antagonistic bacteria MK94‑B2 against red blight of Masson pine and its application, and relates to the field of microbial technology. The present application isolates and obtains antagonistic bacteria MK94‑B2 against red blight of Masson pine from branches of Masson pine, and the deposit number is CCTCC NO: M 20241586. Experiments have confirmed that the strain has a significant inhibitory effect on the conidia germination and germ tube elongation of the red blight pathogen of Masson pine. When treated with MK94‑B2 fermentation filtrate diluted 2.5 times, the spore germination rate of the pathogen of red blight of Masson pine is 0%, and the inhibitory effect is significant, and it can be used to prevent and treat red blight of Masson pine. In addition, the strain has an inhibitory effect on 8 important forest pathogenic fungi, especially on teak brown spot pathogen Alternaria alternata, Masson pine red blight pathogen Lushan pseudodisc multi-hairy spore and poplar leaf multi-septate sclerotinia, and can be used as a broad-spectrum antibacterial agent.

The invention belongs to the field of agriculture, and particularly relates to application of pseudomonas in soil improvement, disease prevention and growth promotion. The preservation number of the pseudomonas is CGMCC No.16020, and the pseudomonas has a bacteriostatic effect on tobacco brown spot pathogenic bacteria, can promote germination of cadmium-polluted alfalfa seeds, and also can improve soil, increase the content of nitrate nitrogen, available phosphorus and / or available potassium in the soil, reduce the content of cadmium and promote growth of alfalfa in the soil seriously polluted by cadmium.

The application belongs to the technical field of microorganisms, and discloses a bacillus velezensis and application thereof in plant disease control. Bacillus velezensis G3, which is preserved in the China General Microbiological Culture Collection Center and has a preservation number of CGMCC No. 36136. The strain has a better bacteriostatic effect on the root rot pathogen of fusarium oxysporum, and also has inhibitory effects on the pathogenic bacteria of the yellow wilt of lycium chinense, tomato gray mold, sclerotinia sclerotiorum, tobacco brown spot, apple anthracnose, apple rot, pear tree dieback and wheat scab, has a broad-spectrum bacteriostatic property, and has a wide application prospect in plant disease control.

The application discloses a fungicidal composition containing manganese edathamil-zinc, tebuconazole and difenoconazole as well as a preparation method and application thereof, relates to the field of pesticides, and includes the following as fungicidal effective components: manganese edathamil-zinc, tebuconazole and difenoconazole, wherein the weight ratio of the manganese edathamil-zinc, tebuconazole and difenoconazole is (0.1-99):(0.1-30):1. The application compounding the active ingredients of the drugs, namely the manganese edathamil-zinc, tebuconazole and difenoconazole, the three components do not produce mutual contradiction, the fungicidal spectrum is enlarged, the fungicidal efficiency is improved, obvious synergistic effect is shown, and the diseases in rice, soybean, wheat, peanut, corn, cotton, sunflower, vegetables and fruit trees can be prevented and treated, especially the diseases such as rust, sheath blight, anthracnose, leaf blight, target spot, brown spot and powdery mildew.

The present application relates to the technical field of pharmaceutical chemistry, and particularly relates to an alpha, beta-unsaturated amide or hydrazide compound containing a carbonyl structure, a preparation method and application. The present application mainly prepares the alpha, beta-unsaturated amide or hydrazide compound containing a carbonyl structure. The compound has a novel structure, and has a good inhibitory effect on plant pathogenic fungi, and particularly has a good inhibitory effect on plant pathogenic fungi such as grape colletotrichum, rice sheath blight, brassica sclerotinia, wheat scab, tomato early blight, tobacco brown spot and eggplant fusarium wilt. The compound has outstanding advantages in the prevention and treatment of plant fungal diseases.