Simultaneous identification of anthracnose, powdery mildew, and brown spot on honeysuckle based on real-time PCR
Through Real-time PCR technology and the design of specific primers and probes using the ITS gene, the problem of rapid identification of the pathogens of honeysuckle anthracnose, powdery mildew and brown spot disease was solved, achieving efficient and accurate pathogen detection and ensuring the stability of honeysuckle yield and quality.
Patent Information
- Application Number
- CN202111582499.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2021-12-22
- Publication Date
- 2025-09-12
- Estimated Expiration
- 2041-12-22
AI Technical Summary
The existing technology lacks systematic and in-depth research on the detection methods of the pathogens of honeysuckle anthracnose, powdery mildew and brown spot disease. The detection technology is lagging behind, making it difficult to quickly and accurately identify them, which affects the yield and quality of honeysuckle.
Real-time PCR technology was used to select the ITS gene as the target gene. Specific primers and TaqMan fluorescent probes were designed. A rapid detection method for identifying the pathogens of anthracnose, powdery mildew and brown spot disease of honeysuckle was established through fluorescent PCR amplification.
It achieves rapid and accurate simultaneous identification of three pathogens in the same tube, improves the resolution and sensitivity of detection, reduces the fluorescence background signal intensity of the fluorescent PCR reaction, and ensures the accuracy and reliability of the detection.