75 results about "Alternaria tenuissima" patented technology

The invention discloses a fungicidal composition containing an alternaria tenuissima-activated protein. The fungicidal composition contains active ingredients A and B, wherein the active ingredient A is alternaria tenuissima-activated protein, the active ingredient B is selected from any one of the following compounds: chitosan, glucosan, lentinan and oligosaccharin, and the weight ratio of the active ingredient A to the active ingredient B is (1: 500)-(500: 1). The composition disclosed by the invention has relatively high activity against a variety of diseases of a variety of crops and an obvious synergistic effect, and a fungicidal spectrum is expanded. The fungicidal composition further has the characteristics of activating metabolic systems of plants, promoting cell growth of the plants and enhancing vitality of root systems.

The invention relates to the field of pesticide preparations, and discloses a sterilization composition and application thereof. The sterilization composition disclosed by the invention comprises an alternaria tenuissima activator protein and an active ingredient A, wherein the weight ratio of the alternaria tenuissima activator protein to the active ingredient A is (1:50)-(30:1), the active ingredient A is selected from amide fungicides, strobilurin fungicides, pyrrole fungicides, triazole fungicides, pyrimidine fungicides, pyridine fungicides or carbamate fungicides, and one or more than two fungicides is selected from each type of fungicides. According to the sterilization composition, the amide fungicides, the strobilurin fungicides, the pyrrole fungicides, the triazole fungicides, the pyrimidine fungicides, the pyridine fungicides or the carbamate fungicides and the alternaria tenuissima activator protein are compounded, and have complementary advantages, thus the resistance of vegetables on diseases can be improved through once application of the sterilization composition, the yield of vegetable crops can be increased, and the amount and frequency of applying the pesticide are reduced.

The invention relates to the field of biotechnology, in particular to application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control. In the invention, endophytic fungus of camphor trees is separated from camphor tree living bodies collected from Tianmu mountain in Hangzhou, Zhejiang province by adopting endophytic fungus separation technology, and isnamed alternaria alternata 31 through microbial taxonomy identification, and the strain is stored in China General Microbiological Culture Collector Center (CGMCCC) on December 31, 2010, and the collection register number is CGMCC No.4508. The invention has a most obvious characteristic that the strain liquid can be fermented to produce active metabolic products which have inhibiting effect on pathogenic bacteria of plant fungus diseases such as Rhizoctonia solani, Fusarium oxysporium, Botrytis cinerea and the like, thus alternaria alternate is an important microbial resource in agriculture.

The invention discloses an application of an activator protein prepared from Alternaria tenuissima by microbial fermentation to a pesticide preparation. The activator protein from Alternaria tenuissima which is prepared from Alternaria tenuissima by fermentation and special extraction processes is a single and stable protein with biological activities. The protein can be prepared with glucosamine oligosaccharides and related protein protectants into wettable powder, the protein can be combined with a receptor protein on a plant cell membrane, in order to induce increases of a series of related enzymatic activity and gene expression quantity in the plant, stimulate a series of metabolism regulation in the plant, promote growth of roots, stems and leaves of the plant, increase chlorophyll content, and increase crop yield. The wettable powder prepared by the protein from Alternaria tenuissima completely accords with each national index, and has the effects for preventing and controlling plant virus diseases, greatly improving physiological functions of plants, enhancing disease resistance of plants, and increasing crop output to a certain degree.

The invention relates to a biological compound preparation for controlling plant virus diseases and an application thereof. The purpose is to provide a biological medicament which can give full play to respective efficacy of alternaria tenuissima activator protein and amino-oligosaccharin so as to greatly improve the activity of the medicament and realize more effective control of the generation of plant virus diseases. The preparation of the invention is prepared by compounding alternaria tenuissima activator protein mother liquor with a concentration of 1000 mg/L and amino-oligosaccharin mother liquor with a concentration of 1000 mg/L according to a volume ratio of 1:(0.5-2.0); and when the volume ratio is 1:1, the compound preparation has the highest activity and the best control effect.

The invention relates to a method for rapidly detecting alternaria tenuissima ketone acid. The method is based on a horse radish peroxidase catalysis luminol-hydrogen peroxide luminescence system, indirect competitive chemiluminescent enzyme-linked immunosorbent assay of the alternaria tenuissima ketone acid is built, and the method has the advantages of high sensitivity of a chemiluminescence and high specificity of an immunoassay. The detection range of the method is 0.032ng/mL-30.244ng/mL, IC50 (half maximal inhibitory concentration) is 0.973ng/ mL, lowest detection limit is 0.010ng/mL, a linear regression equation is y=49.82-20.14x, and R20=0.9966. Compared with a traditional ELISA (enzyme-linked immunosorbent assay) method, the IC50 and the lowest detection limit are lower and high in specificity, duration time of light-emitting signals is long and can reach several hours, linear detection range is wider, and the method provides bases for research of limit standards of the alternaria tenuissima ketone acid, detection of pollution level of the alternaria tenuissima ketone acid and risk analysis.

The invention provides a biofertilizer containing amino-oligosaccharin and fermented animal dung. The biofertilizer comprises the following raw materials: fulvic acid, peat, tartaric acid, fermented cow dung, broad bean shell, chloroprene rubber powder, amino-oligosaccharin, Alternaria tenuissima activated protein, D-limonene, mung bean plant, medical stone, nano anion powder, wheat dietary fiber, Lactobacillus zeae, and Halobacterium salinarium. The invention also provides a preparation method of the fertilizer. After application of the fertilizer to saline-alkali soil, six months later, 0-30cm soil layer salinity is 0.18-0.34%, the soil pH is 7.0-7.5, the total nitrogen content of the soil is 0.091-0.127%, the breathable porosity of the soil is 13-17%, the mechanical stability of 5-7mm water-stable granules in the soil is 507-515g, the water-holding capacity of the soil is 20-28%, and the available potassium content of the soil is increased by 45-60%.

The invention belongs to the technical field of pesticides, and particularly relates to a plant growth regulator and an application method thereof. Effective components of the plant growth regulator comprise alternaria tenuissima activated protein and any one selected from diethyl aminoethyl hexanoate, zeatin and compound sodium nitrophenolate. According to the plant growth regulator, and wettablepowder, water dispersible granule, suspension and water aqua thereof, the alternaria tenuissima activated protein and any one selected from diethyl aminoethyl hexanoate, zeatin and compound sodium nitrophenolate are mixed with each other to be used as the effective components, and the action mechanisms are different; after compounding, a synergistic effect is achieved under a defined usage concentration and usage method according to various crops such as rice, corns, wheat, peanuts, cotton, vegetables, tobaccos, fruit trees, gardens, flowers and the like, and the yield and the income are increased; an obvious weight-increasing effect is achieved for fruits of the fruit trees, and the chlorophyll content of plants is effectively improved; and moreover greenness preservation and an anti-aging effect are achieved, the sugar content is improved, growth of the crops is promoted, and quality of the fruits is effectively improved.

The invention discloses alternaria tenuissima, which is classified and named as alternaria tenuissima of deuteromycotina alternaria with a bacterial strain number of JSNL005-1, and is already preserved in the general microbiology center of China microbe preservation management committee with a preservation number of CGMCC No. 11311 and a preservation date of September 11, 2015. The alternaria tenuissima JSNL 005-1 disclosed by the invention has remarkable effects in aspects of improving growth rate and drought resistance ability of the dendrobium officinale, and the artificial culture can be realized; the reproductive speed is fast; the alternaria tenuissima is convenient for large-scale production, and is good in development and application prospect.

The invention provides a bactericidal composition and applications thereof, and belongs to the field of combination of pesticides. The effective components of the bactericidal composition are methiadinil (A) and biostimulant (B), and the weight ratio of (A) to (B) is 10 : 0.01 - 0.01 : 10; the biostimulant (B) is humic acid, a seaweed extract product, an alternaria tenuissima activator protein orbeta-lupin globulin polypeptide; the compounding of the effective component (A) and (B) has obvious synergism, so that the application amount of each single dosage can be effectively reduced. The composition can add auxiliary components that are allowed to use in other pesticides and be processed into any dosage form; the composition is used for enhancing the autoimmune abilities of plants, so that the prevention of viral diseases, fungal and bacterial pathogens can be achieved; and the composition is mainly used for preventing fungal and bacterial diseases such as downy mildew, phytophthora,anthrax, early blight, rice blast, wheat powdery mildew, leaf spots of banana, viral diseases, bacterial angular spot diseases and soft rot diseases.

The invention relates to the application of a compound to the prevention and treatment of plant fungal diseases caused by alternaria alternata and particularly relates to the application of 2-methyl-5-isopropyl phenol to the prevention and treatment of plant fungal disease caused by alternaria alternate, belonging to the technical field of pesticides. An OP-10 type emulsifying agent is added into the 2-methyl-5-isopropyl phenol according to the proportion of 1-45% of the total volume and dissolved by acetone to prepare a 1-80% (w/w) 2-methyl-5-isopropyl phenol solution used for preventing and treating the tomato early blight, cabbage black spot, tobacco brown spot caused by alternaria alternata. The 2-methyl-5-isopropyl phenol has a strong function of inhibiting various pathogenic alternaria alternata and a good effect of inhibiting the plant fungal diseases caused by various pathogenic alternaria alternata, has a high activity and can take effect quickly, thereby being a novel pesticide for preventing and treating the plant fungal diseases caused by the alternaria alternata.

The invention discloses a method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus. The method comprises following steps: preparation of a fluid nutrient medium; culturing of alternaria alternata; preparation of alternaria alternata toxin, and application of alternaria alternata toxin, wherein active toxin is prepared into a mixed solution with a concentration ranging from 125 to 2000<mu>g/ml, and the mixed solution is sprayed onto Alternanthera philoxeroides, so that obvious toxic symptoms of Alternanthera philoxeroides can be observed in three days, and weeds are killed. The alternaria alternata toxin possesses killing effect on most symbiotic weeds of Alternanthera philoxeroides. Operation of the preparation method of the liquid nutrient medium is simple; culture medium raw materials are easily available; needed equipment is simple; stability of the used toxin is high; extraction purification technology is simple; large-scale batch production can be realized; added accessory ingredients possess excellent synergistic effects on alternaria alternata toxin; the alternaria alternata toxin is applied via stem and leaf spraying; application is convenient; target weed Alternanthera philoxeroides can be killed effectively, and most symbiotic weeds can be killed at the same time; and environmental safety of the method is relatively high.

The invention discloses a loquat endophytic unciform trichoderma strain and applications of the loquat endophytic unciform trichoderma strain. The endophytic trichoderma strain is obtained through separation, then morphological and molecular biological identification is carried out, meanwhile, the antibacterial activity of the endophytic trichoderma strain is tested, and thus a material is provided for the development and utilization of the strain; the endophytic trichoderma strain has the antibacterial activity for five phytopathogens, wherein the inhibiting effect for fungi is obviously stronger than that for bacteria, specifically, the inhibiting effect for pyricularia grisea is the strongest and is 83.17%, the antibacterial rate for alternaria tenuissima takes the second place and is 75.10%, the antibacterial rates for fusarium proliferatum and fusarium sambucinum have no obvious differences and are respectively 52.02% and 49.07%, and the antibacterial rate for Xanthomonas oryzae pv.oyzae is the poorest, and is 38.24%. The trichoderma strain has the antibacterial wide spectrum, and has the development and utilization values and prospects.

The invention discloses a Huperzia serrata endophytic fungus resisting Botrytis cinerea. The fungus is classified and named Alternaria alternata HR38-7, and is preserved in the China Center For Type Culture Collection, with the preservation number of CCTCC No. 2019388. The strain is screened from the Huperzia serrata endophytic fungus for the first time, and can be used for preventing and controlling the Botrytis cinerea of crops, and can be used as an active ingredient for preparing a biocontrol preparation for preventing and controlling the Botrytis cinerea of crops.

The invention relates to the technical field of microorganisms, mainly relating to a hypha extract of a strain of endophyte separated from wild ginseng fibrous roots in the Changbai mountain under artificial culturing conditions and application of the hypha extract in prompting the growth of corns, improving the stress resistance and increasing the yield. The strain is preserved in China general microbiological culture collection center, and the category name of the fungus is Alternaria tenuissima. The invention aims to prompt the growth of the root system of corns, increase the utilization ratio of the fertilizer, improve the drought resistance and increase the yield of corns by the application of related products.

The invention relates to a bactericidal composition containing an alternaria tenuissima activator protein. The alternaria tenuissima activator protein and a compound A serve as active ingredients, andthe compound A is a tetrahydro-beta-carboline compound containing an acylhydrazone structure. The composition can be used for controlling viral diseases of fruit trees, vegetables and field crops andcan achieve the effects of effectively improving stress resistance of the crops, regulating growth of the crops, promoting flowering, improving yield, improving the fruit quality and the like.

The invention relates to a yield increasing method of Alternaria tenuissima activator protein. According to the method, spores are prepared, a spore suspension liquid is prepared, and beta-sitosterol and betaine are added to a fermentation culture medium when a strain liquid is cultured, so that the yield of Alternaria tenuissima activator protein is increased. With the adoption of the yield increasing method, the culture medium and the culture conditions are optimized, so that the activator protein is increased by four times or more; ingredients of the culture medium all come from plants, the sources are convenient and safe, the cost is low, and large-scale production is facilitated; dynamic parameters of fermentation in a shaking flask are researched, and a fermentation culture medium suitable for strains to grow is acquired; influences of the inoculation amount, the initial pH value of the fermentation culture medium, the quantity of liquids in the shaking flask, the temperature and the like on fermentation are researched, and the composition of Alternaria tenuissima activator protein culture medium and the optimum fermentation culture conditions in the shaking flask are determined.

The invention discloses a Fermentation method of alternaria tenuissima. The fermentation method comprises the following steps: taking out an alternaria tenuissima culture HZ-1 stored at 4 DEG C; picking up part of bacterial colony and inoculating the same to a fresh PDA culture medium to be cultured, then picking up part of the bacterial colony from the culture medium and inoculating the same to afresh PDA culture medium, and repeating the step 2-3 times to obtain an activated HZ-1 culture which is vigorous in growing power; taking the HZ-1 culture cultured for 7d in a petri dish, perforating8mm bacterial cakes by a perforator, and inoculating the bacterial cakes 10/L into the PDA liquid culture medium; and culturing the cakes in an oscillator, and filtering the mixture to obtain a HZ-1fermenting liquid. According to the fermentation method, the culture HZ-1 is reproduced massively, thereby laying a theoretical foundation for industrial production and further popularization and application of a biological fungicide.

The present invention relates to protein, and features that inhibin gene phb is cloned from the cDNA library of Alternaria tenuissima and expressed in Pichia pastoris and colibacillus and the expressed prohibitin has the functions of antagonizing diseases and promoting the growth of plant.

The invention discloses a mulch type green manure for an orchard. The mulch type green manure is prepared from the following raw materials by weight: 80-100 parts of the surface soil of the orchard, 50-60 parts of the paste of stems and leaves of legume crops, 10-20 parts of the paste of fallen leaves, 10-20 parts of the paste of weeds, 1-10 parts of the paste of fallen fruit, 5-10 parts of a starch graft copolymer, 2-4 parts of the protein of Alternaria tenuissima, 1-3 parts of amino-oligosaccharin and 1-5 parts of enzyme microorganisms. The mulch type green manure of the invention realizes the resource utilization or harmless treatment of agricultural production waste, realizes the modern ecocycle agriculture of conservation and intensive utilization before production, clean production and pollution control during production and waste utilization after production, can realize prevent and control pests and diseases in the orchard and reduce the use of inorganic fertilizers and pesticides, and provides abundant nutrients for soil. The mulch type green manure achieves the prevention and control of pests and diseases in the orchard, and decreases the use of inorganic fertilizers andpesticides.

The invention relates to the technical field of plant disease prevention and treatment, in particular to a fungicide composition for preventing and treating plant fungal diseases caused by alternariaalternata and a preparation method and use of the fungicide composition. The fungicide composition for preventing and treating the plant fungal diseases caused by the alternaria alternata is preparedfrom the components in parts by weight: 20 parts of endophytic streptomyces violascensfermentation liquid of ginkgo biloba, 30-50 parts of an exotesta extract of the ginkgo biloba and 1-2 parts of Tween 80, and the fungicide composition is used for preventing and treating the plant diseases caused by the alternaria alternata after being diluted with water for 100-150 times. The preparation methodof the fungicide composition comprises the steps of the extraction of the exotesta extract of the ginkgo biloba, the preparation of the endophytic streptomyces violascensfermentation liquid of the ginkgo biloba and the compounding of the endophytic streptomyces violascensfermentation liquid of the ginkgo biloba and the exotesta extract of the ginkgo biloba. According to the fungicide composition,the incidence and disease indexes of the plant diseases caused by the alternaria alternata can be effectively lowered, the relative inhibition rate is increased, the excellent prevention and treatmenteffect is achieved, and the advantages of stability, low toxicity, no residue, no environmental pollution and the like are achieved.

The invention discloses an alternaria alternata strain and an application of the alternaria alternata strain in resisting pathogenic bacteria of potato ring rot. The invention provides alternaria alternata SHM-1, which is assigned with the accession number of CGMCC No.15984. The invention further protects a preparation method of an extract. The preparation method comprises the following steps: (1)culturing the alternaria alternata SHM-1; (2) collecting a filtrate, carrying out concentrating, and thus a concentrated solution is obtained; (3) carrying out organic phase extracting, and collecting an organic phase; and (4) carrying out reduced pressure distillation, so that the extract is obtained. The extract prepared by adopting the method also belongs to the protection scope of the invention. The invention further protects the application of the alternaria alternata SHM-1 in preparing an antagonist for the pathogenic bacteria of bacterial plant diseases. The invention further protectsan application of the extract, and the application is (a) or (b): (a) preparing the antagonist for the pathogenic bacteria of bacterial plant diseases; and (b) serving as the antagonist for the pathogenic bacteria of the bacterial plant diseases. With the technical scheme provided by the invention, a novel path is provided for preventing and treating the diseases caused by the pathogenic bacteriaof the potato ring rot.

The invention discloses an application of itraconazole in the preparation of fungicides for the prevention and control of plant pathogenic bacteria. Experiments prove herein that the itraconazole hasgood inhibitory activity against the main diseases of important grain crops, including fusarium graminearum causing wheat scab, magnaporthe oryzae causing rice blast, rhizoctonia cerealis causing sheath blight and curvularia maize causing maize leaf spot; moreover, the itraconazole has good inhibitory activity against the physalospora piricola causing apple ring rot, valsa mali causing apple rot and alternaria tenuifolia causing leaf spot disease of fruits and vegetables; the itraconazole also has good inhibitory activity against soil-borne diseases that are extremely difficult to prevent andcontrol in agricultural production, such as fusarium oxysporum causing blight, and the inhibitory activity is even better than those of traditional fungicides such as carbendazim and tebuconazole. Forthe first time, the itraconazole, a green small-molecular compound screened through three-dimensional structures of target proteins, is applied to the prevention and control of important plant diseases in agricultural production and has a good market application prospect.

The invention discloses a bactericidal composition containing alternaria tenuissima activator protein and zhongshengmycin and application of the bactericidal composition, and relates to the field of agricultural chemicals. The bactericidal composition comprises two active components: alternaria tenuissima activator protein and zhongshengmycin, wherein the weight ratio of the alternaria tenuissima activator protein to the zhongshengmycin is (0.5-3.5): 1. Test results show that the bactericidal composition provided by the invention has obvious synergism, the application amount is reduced, and the use cost is reduced. According to the bactericidal composition, bactericides with different action mechanisms and action modes can be compounded, the application amount of each single agent is effectively reduced, and the bactericidal composition has very good effects in the aspects of enlarging a bactericidal spectrum, delaying the drug resistance of bacteria and fungi, improving the prevention and treatment effect and the like.

The invention discloses a monoclonal antibody for identifying alternaria tenuissima, and a hybridoma cell strain of the monoclonal antibody. The monoclonal antibody for identifying alternaria tenuissima is secreted and generated by a hybridoma cell strain a the preservation number of CCTCC No:C2019228. The hybridoma cell strain is named as a hybridoma cell strain AtD2, is preserved in China Centerfor Type Culture Collection (CCTCC) on Oct.17,2019, and the preservation number of the hybridoma cell strain is CCTCC No:C2019228. The monoclonal antibody is used for identification and dynamic monitoring of animal and plant diseases caused by infection of alternaria tenuissima and biological research of alternaria tenuissima, and a large amount of the monoclonal antibodies can be obtained by carrying out intraperitoneal injection on a BaLb/c mouse. Compared with polyclonal antibodies, the monoclonal antibody has the advantages of high purity, strong specificity, good repeatability and the like.