Loquat endophytic unciform trichoderma strain and applications thereof

A technology of Trichoderma uncinate and Trichoderma, which is applied in the field of microorganisms, can solve problems such as destroying the environment and ecology, disease resistance, and threatening the safety of humans and animals.

Active Publication Date: 2017-03-22
YUNNAN RUNJIE AGRI SCI & TECHCO
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The object of the present invention is to provide a loquat endophytic Trichoderma hacinatum strain and its application, aiming to solve the problems of damaging the environment and ecology, threatening the safety of humans and animals, and bringing about disease resistance by chemical control of plant diseases

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  • Loquat endophytic unciform trichoderma strain and applications thereof
  • Loquat endophytic unciform trichoderma strain and applications thereof

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Embodiment Construction

[0011] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0012] The application effects of the present invention will be described in detail below in conjunction with experiments.

[0013] 1. Materials and methods

[0014] 1.1 Bacteria to be tested

[0015] The endophytic Trichoderma strain was isolated from the trunk of loquat in Mengzi, Yunnan, and the strain number was YNMZT1 preservation number CCTCC NO: M2016366, and was identified by morphology and ITS-PCR. The four phytopathogenic fungal strains were Magnaporthe oryzae, Alternaria tenopolaris of Dendrobium officinale black spot, Fusarium elderii and Fusarium laminarum root rot pathogens of Dendrobium officinale; one stra...

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Abstract

The invention discloses a loquat endophytic unciform trichoderma strain and applications of the loquat endophytic unciform trichoderma strain. The endophytic trichoderma strain is obtained through separation, then morphological and molecular biological identification is carried out, meanwhile, the antibacterial activity of the endophytic trichoderma strain is tested, and thus a material is provided for the development and utilization of the strain; the endophytic trichoderma strain has the antibacterial activity for five phytopathogens, wherein the inhibiting effect for fungi is obviously stronger than that for bacteria, specifically, the inhibiting effect for pyricularia grisea is the strongest and is 83.17%, the antibacterial rate for alternaria tenuissima takes the second place and is 75.10%, the antibacterial rates for fusarium proliferatum and fusarium sambucinum have no obvious differences and are respectively 52.02% and 49.07%, and the antibacterial rate for Xanthomonas oryzae pv.oyzae is the poorest, and is 38.24%. The trichoderma strain has the antibacterial wide spectrum, and has the development and utilization values and prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an endophytic Trichoderma hacinata strain of loquat and application thereof. Background technique [0002] Plant disease is one of the important factors leading to the loss of agricultural production, and chemical control has always been the main way of disease control. However, the use of a large number of chemical pesticides has brought a series of problems to environmental ecology, human and animal safety, and disease resistance, which prompts people to seek a safe, environmentally friendly and effective disease control strategy. A large number of studies have shown that some microorganisms or their preparations have the effect of promoting growth and preventing plant diseases, such as Trichoderma, Chaetomium, Shell and other fungi and bacteria of the genus Bacillus, among which Trichoderma is the most widely used organism. Anti-bacterial. Trichoderma spp. is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P1/00C12R1/885
CPCA01N63/30C12N1/145C12R2001/885
Inventor 孔琼袁盛勇刘艳红田学军薛春丽李珣陶宏征周银丽
Owner YUNNAN RUNJIE AGRI SCI & TECHCO
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