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64 results about "Trichoderma spp" patented technology
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Trichoderma spp.(BOX 1) are free-living fungi that are. highly interactive in root,soil and foliar environments. It has been known for many years that they produce a. wide range of antibiotic substances 1 and that they par-. asitize other fungi (BOX 2) .They can also compete with.
Solid-chemical composition for sustained release of organic substrates and complex inorganic phosphates for bioremediation
InactiveUS6620611B2Improve solubilityIncreasing speed and effectivenessBacteriaWater treatment compoundsPseudomonasTrichoderma spp
Owner:HINCE ERIC CHRISTIAN MR
Biological organic repairing agent for soil pollution and application and preparation method thereof
InactiveCN105347884AReduce absorptionReduced enrichmentBio-organic fraction processingExcrement fertilisersBiotechnologyPollution
The invention relates to the field of soil fertilizer and provides a biological organic repairing agent for soil pollution and its application and preparation method. The biological organic repairing agent of the invention is composed of, by weight, 1.5-3 parts of eymogenic bacterium, 1-1.5 parts of wheat bran, 1.5-2 parts of molasses meal, 45-50 parts of ammonium humate, 45-50 parts of hydrolase dregs, 150-200 parts of soybean meal, 100-150 parts of humic acid powder, 80-100 parts of zeolite powder, 25-40 parts of a calcium magnesium phosphate fertilizer, 150-200 parts of cattle and sheep manure, 150-204 parts of plant straw, 25-40 parts of potassium humate, 1.5-3 parts of bacillus mucilaginosus, 1-1.5 parts of Gliocladium roseum, 1.5-2 parts of Streptomycesvinaceus-drappus, 1-1.5 parts of trichoderma spp, 1-1.5 parts of Paecilomyces lilacinus, 1-1.5 parts of bacillus subtilis and 1-1.5 parts of micrococcus. According to the invention, soil pollution control, improvement and nutrition are integrated.
Owner:HEBEI CIXIN ENVIRONMENTAL PROTECTION TECH CO LTD
Microbial formulation and method of using the same promote plant growth
A plant growth promoting composition includes beneficial microbes and microbial activators. The beneficial microbes may include Bacillus spp., Azotobacter spp., Trichoderma spp., Saccharomyches spp., or combinations of these, the microtrial activators include enzymes, metabolites, carbohydrate yeast, humic material, staich, amino acids and micornutrients. The plant growth promoting composition may also include organic and inorganic fertilizers.
Owner:ULTRA BIOTECH
Method for producing trichoderma chlamydosporium
A trichoderma spp chlamydospore production method of obtaining trichoderma spp from crop root surface soil is disclosed. The method obtains chlamydospore through controlling nutrition condition , adding chlamydospore promotor, regulating other technical parameter to control the fermentation condition. First the trichoderma spp from corp root is purified and then transferred to tube slant as stock bacteria, then vibrated in triangular tube to become bacteria liquid, at last fermented in fermenter to produce chlamydospore suspension liquid. The merit of inventive chlamydospore is long life.
Owner:SHANGHAI JIAO TONG UNIV
Microbial organic fertilizer, and preparation method and purpose thereof
ActiveCN103588544AImprove the effect of disease controlReduce the disease indexOrganic fertilisersFertilizer mixturesPlant diseaseTrichoderma harzianum
The invention provides a preparation method of a microbial organic fertilizer. The method comprises the following steps: (1) chlamydospore preparation by carrying out submerged fermentation on Trichoderma harzianum strains with preservation number of CGMCC NO:5812 to produce chlamydospores; (2) manure particle preparation by mixing manure, humic acid and water, and inoculating deodorizing bacteria for fermentation deodorization and granulating and drying to obtain the manure particles; and (3) mixing the manure particles and the chlamydospores. The invention also provides a microbial organic fertilizer obtained from the above preparation method. The invention also provides purpose of the microbial organic fertilizer in control of plant diseases. Through the above technical scheme, the invention can significantly improve the disease control effect of Trichoderma spp.
Owner:珠海横琴协同投资管理有限公司
Trichoderma spp. strain antagonizing soil-borne diseases
InactiveCN103484376AFast growthHigh sporulationFungiMicroorganism based processesDiseaseTrichoderma asperellum
Owner:上海大井生物工程有限公司
Trichoderma wettable powder and preparation method thereof
The invention provides a Trichoderma wettable powder and a preparation method thereof. The method comprises the following steps: mixing Trichoderma liquid, a filling material and a raw powder protective agent, drying to obtain the Trichoderma raw powder, crushing an auxiliary agent containing a carrier, a dispersant, vitamin C, a sticker and a wetting agent and mixing; mixing the Trichoderma raw powder and the auxiliary agent, and drying to obtain the Trichoderma wettable powder. The method of the invention employs liquid fermentation, can be directly used for preparing the wettable powder; dextrin and vitamin C are added as the protective agents, viability stage of Trichoderma can be protected and prolonged under room temperature and drying condition, loss rate of Trichoderma is greatly reduced, shelf period of the product is increased; the raw powder and the auxiliary agent are separately prepared, Trichoderma spore mixing and stirring time can be reduced, Trichoderma spp loss rate is greatly reduced; the materials of the auxiliary agent are chemical substances, so that large scale production can be realized, and Trichoderma survival rate is not influenced.
Owner:SHANGHAI WANLIHUA BIOTECH
Method for preparing distillers' grain protein feed through three-stage mixed bacterium solid state fermentation
The invention belongs to the technical field of microbial fermentation, and concretely relates to a method for preparing a distillers' grain protein feed through three-stage mixed bacterium solid state fermentation. The method comprises the following steps: 1, first stage fermentation: carrying out high temperature fermentation on an A group bacillus combined seed liquid at 60DEG C with distillers' grains as a substrate to obtain a distillers' grain fermentation material; 2, adding locust tree leaf powder, poplar tree leaf powder and wheat bran to the distillers' grain fermentation material obtained after the first stage fermentation, inoculating a Geotrichum candidum, yeast and Trichoderma spp. seed liquid, and blending to obtain a fermentation material, and carrying out second stage fermentation; and 3, inoculating a final product obtained after the second stage fermentation to a B group bacillus combined seed liquid, carrying out third stage fermentation to obtain a final fermentation product fresh distillers' grain protein feed, or drying, crushing, granulating and packaging to obtain a particulate distillers' grain protein feed. The content of crude proteins in the distillers' grain protein feed prepared by adopting the method is 29-32% averagely, and is 58-74% higher than the content of crude proteins in dry distillers' grains.
Owner:FUJIAN NORMAL UNIV
Method for producing biological organic fertilizer by using livestock manure
InactiveCN101250065ASolve pollutionPromote sustainable developmentClimate change adaptationExcrement fertilisersBiotechnologyFeces
A method for using livestock manure to produce organic fertilizer belongs to the technical field of agriculture and environment sciences. The invention uses livestock manure as raw material and the preparation method comprises mixing and stocking straw powder and aged compost, grafting livestock manure efficient mature bacterial, to complete high-temperature fermentation in short time, thereby realizing harmless and resource recovery, and leading beneficial microbes as phosphorus bacterial, silicate bacterial, photosynthetic bacterial and trichoderma spp or the like into a fermentation system at the later period of fermentation, to obtain biological organic fertilizer. The invention can produce biological organic fertilizer of agriculture planting industry and resolves the pollution of livestock manure.
Owner:SHANGHAI ACAD OF AGRI SCI
Botanical bactericidal preparation and application of preparation in blight control
ActiveCN102388923AImprove applicabilityRaw materials are easy to getBiocidePlant growth regulatorsPest controlMildew
Owner:JIANGSU ACAD OF AGRI SCI
Special fertilizer for ornamental flower plants
The invention discloses a special fertilizer for ornamental flower plants, relates to a special fertilizer for flowers, and belongs to the technical field of biologic fertilizers. The fertilizer is characterized in that the fertilizer comprises 25-35wt% of Trichoderma spp., 15-20wt% of urea, 12-13wt% of ammonium biphosphate, 12-13wt% of potassium oxide, 6-13wt% of attapulgite powder, 10-15wt% of humic acid and 1-2wt% of amino acid chelated rare earth. The fertilizer has the characteristics of water retention, fertilizer retention, long fertilizing time and good ventilating performance, is benefit for the root growth and the absorption and utilization of phosphorus by plants, and can effectively prevent ammonia and potassium loss and prevent the fixation of soil plant active mineral components by phosphoric acid to make soil in a loosened ventilating state for a long time, so the growth of crops is promoted.
Owner:苏丽娟
Trichoderma spp. strain antagonizing maize stem rot and sheath blight and application thereof
Owner:上海大井生物工程有限公司
Preparation method of compound bacterium fermentation product for preventing and treating plant phytophthora, compound bacterium fermentation product and application thereof
The invention belongs to the technical field of biological prevention and treatment, and particularly relates to a preparation method of a compound bacterium fermentation product for preventing and treating plant phytophthora, the compound bacterium fermentation product and application thereof. The preparation method comprises the following steps: (1), performing spore culturing on marine trichoderma spp., and inoculating the obtained spores into an oscillation culture medium for oscillation culture to obtain a marine Trichoderma spp. oscillation culture; (2), performing seed culturing on bacillus subtilis, inoculating the obtained seed culture into an oscillation culture medium for oscillation culture, mixing the bacillus subtilis oscillation culture with the marine trichoderma spp. oscillation culture obtained in the step (1), carrying out co-fermentation to obtain a co-fermentation product, and centrifuging and filtering the co-fermentation product to obtain a compound bacterium fermentation product. The compound bacterium fermentation product prepared by the invention remarkably reduces the growth of plant phytophthora, and has a better prevention and treatment effect on plantfungal diseases compared with the prevention and treatment effect of a single strain.
Owner:TOBACCO RES INST CHIN AGRI SCI ACAD
Production process for fermenting trichoderma spp by taking orange peels as raw materials
ActiveCN103468629AHigh sporulationAchieve high density cultureBiocideContaminated soil reclamationBiotechnologyTrichoderma sp.
Owner:INST OF SOIL SCI CHINESE ACAD OF SCI
Application of improved ATMT (Agrobacterium Tumefaciens-Mediated Transformation) to construction of trichoderma atroviride T23 deltaCrel
ActiveCN105368866ARepress transcriptionInhibition of secretionFungiMicroorganism based processesEscherichia coliCarbon metabolism
The invention discloses an application of improved ATMT (Agrobacterium Tumefaciens-Mediated Transformation) to construction of trichoderma atroviride T23 deltaCrel. The application is characterized in that the genomic DNA of trichoderma atroviride T23 is taken as a template, PCR (Polymerase Chain Reaction) amplification is carried out on the Crel gene sequence, and thus upstream and downstream flanking sequences of the Crel gene are obtained; then leading in respectively to knock out upstream and downstream of plasmid pC1300qh, so as to form recombinant plasmid pC1300qh-deltaCrel; converting escherichia coli competence of the recombinant plasmid pC1300qh-deltaCrel, performing verification, screening positive clone, extracting recombinant plasmid pC1300qh-deltaCrel, leading in agrobacterium tumefaciens AGL1, and carrying out improved ATMT. By using the improved ATMT technique, a trichoderma atroviride carbon metabolism inhibiting factor CRE1-knocked-out mutant strain is successfully constructed; the T23 deltaCrel bacterial strain has the functions of promoting secretion and activity of degrading enzyme of cell walls of trichoderma spp., and indirectly improving the function of trichoderma spp. to antagonize pathogenic bacteria.
Owner:SHANGHAI JIAO TONG UNIV
Fertilizer synergist and preparation method thereof
InactiveCN104591843APrevent compactionRestorationMagnesium fertilisersAlkali orthophosphate fertiliserTrace element compositionTrichoderma sp.
Owner:YANTAI NAKE ENVIRONMENTAL PROTECTION TECH
Making method of candied Chinese chestnuts
InactiveCN107996792APrevent oxidation and discolorationGood colorConfectionerySweetmeatsAmylaseMicrowave
The invention discloses a making method of candied Chinese chestnuts. The making method comprises the following steps of (1) pretreating raw materials: removing shells from Chinese chestnuts, stripping out coats, and boiling the treated Chinese chestnuts with water for 3-5min; (2) performing color protection: immersing the boiled Chinese chestnuts in a color protecting liquid containing a chestnutshell extract, wherein in the color protecting liquid, the percentage by mass of a Chinese chestnut extract is 20-25%; (3) performing enzyme treatment: placing the Chinese chestnuts obtained in step(2) in enzymatic hydrolysate containing amylases and a trichoderma spp fermented product for enzymolysis; (4) performing treatment in a sugar solution: placing the Chinese chestnuts after enzyme treatment in the sugar solution, performing sugaring with microwaves for 30min, and the sugar solution consists of the following raw materials in percentage by mass: 10-20% of glucose, 5-10% of a persimmoncolloid extract, and the balance of water; and (5) performing drying: drying the Chinese chestnuts treated in the sugar solution through microwave drying until the water content is 8-12%. The made candied Chinese chestnuts are good in shaping, not liable to break, low in hardness and good in texture structure.
Owner:广西驰胜农业科技有限公司
Production of trichoderma spp. microbial fertilizer by waste water of kitchen garbage
The invention relates to a production method of a trichoderma spp. biocontrol agent, and belongs to technical fields of microbial fertilizers and the resource utilization of waste water. According to the method, grape peel dregs and grain stillages produced during grape wine production are used as raw materials for the production, and a method of solid state fermentation is adopted to produce the trichoderma spp. biocontrol agent. The production process comprises: (1) taking waste water of kitchen garbage as a medium to inoculate trichoderma spp. and preparing liquid seeds by liquid culture; (2) inoculating the liquid seeds to prepare a large number of liquid trichoderma spp. products with waste water of kitchen garbage as raw materials for the fermentation; (3) adding the cultured liquid seeds or the liquid trichoderma spp. products into biomass materials, and performing solid state fermentation to prepare solid trichoderma spp. products; (4) drying the fermented products, adding stabilizing agents and auxiliary agents, pulverizing, and packaging. The process for producing trichoderma spp. microbial fertilizers has low production cost and is environment-friendly; the products can significantly improve the quality of soil environment, increase crop yields, and control various plant diseases caused by fungi. A processing method for effectively processing plenty of high concentration waste water produced during the production of kitchen garbage is simultaneously realized and environmental pollution is reduced.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Microbial straw fermentation agent and method for producing straw forage by utilizing fermentation agent
The invention discloses a microbial straw fermentation agent and a method for producing a straw forage by utilizing the fermentation agent, and concretely relates to a microbial straw fermentation agent used for fully fermenting straw for forming a forage and a method for producing a straw forage by utilizing the fermentation agent. The to-be-solved technical problem is that the current quantity of straw used as a forage is relatively small, majority of straw is burned on site, air is polluted and resource is waste. The microbial straw fermentation agent comprises an anaerobic bacteria fermentation agent and an anaerobic bacteria fermentation agent, wherein the anaerobic bacteria fermentation agent is microzyme, trichoderma spp and bacillus cereus, and the anaerobic bacteria fermentation agent is lactobacillus bifidus. By employing the technical scheme, the microbial straw fermentation agent improves the palatability of a fermented straw forage, substantially reduces the crude fiber content of the straw forge, improves the digestion absorption rate of ruminants, and reduces stomach and intestine disease incidence of animals. Working intensity of workers is mitigated, fermentation cost is reduced, and digestion rate is improved.
Owner:JIYUAN JIABAINONG BIOTECH CO LTD
Chlamydospore obtained through liquid fermentation of trichoderma spp and preparation method of chlamydospore
PendingCN110591921AHigh sporulationImprove labor productivityFungiMicroorganism based processesHydrolysateFermentation
The invention relates to chlamydospore obtained through liquid fermentation of trichoderma spp and a preparation method of the chlamydospore. The chlamydospore is obtained through the steps of using corn straw enzymatic hydrolysate as a culture medium raw material, wherein the straw enzymatic hydrolysate contains sugar components, dispersing agent components and the like, inoculating straw enzymatic hydrolysate culture mediums with trichoderma spp cakes, and performing liquid fermentation so as to obtain the chlamydospore. According to the chlamydospore and the preparation method thereof disclosed by the invention, the corn straw enzymatic hydrolysate is used as a main raw material, by methods of adding vitamins, trace elements, dispersing agents and the like, the spore production quantityof the chlamydospore is substantially increased, the production cost is reduced, the fermentation period is also shortened by 2 days, the working and production efficiency is improved, besides, the shelf life can reach 1 year, and in addition, promotion and application of a trichoderma spp agent are guaranteed.
Owner:YANGZHOU POLYTECHNIC INST
Novel microbial enzymes and their use
InactiveCN101137749ABiochemical treatment with enzymes/microorganismsOxidoreductasesBiotechnologyMicroorganism
Owner:VALTION TEKNILLINEN TUTKIMUSKESKUS
Application of Trichoderma harzianum LTR-2
The invention relates to the technical field of microorganisms, in particular to a method for preparing a metabolite for controlling tobacco mosaic virus disease and its application. The invention provides a Trichoderma harzianum LTR-2 to control tobacco mosaic virus disease, more specifically application of Trichoderma harzianum LTR-2 metabolites in the control of tobacco mosaic virus disease. The invention obtains a strain for controlling tobacco mosaic virus disease from Trichoderma spp. stored in laboratory, and carries out virus control research on the metabolite of the strain so as to directly and rapidly control tobacco mosaic virus disease, ensure tobacco yield and quality, and provide reference for maintaining microbial community balance in tobacco planting.
Owner:ECOLOGY INST SHANDONG ACAD OF SCI +1
Soybean root rot disease composite biocontrol fungi granule and preparation method thereof
The invention belongs to the field of agricultural production technologies. It is a composite biocontrol fungi particles agent of soybean root rot and its preparation method. The invention is granular formulations and its production process which takes the four Trichoderma and Chaetomium fungi separated from natural soil as the mainly effective ingredients and is mainly used for the prevention and treatment of bean root rot. The present invention changes the disorder prevention and cure limits of applying biocontrol agents made of a single Trichoderma, in the invention there are multiple biocontrol fungi which has preventing and curing effect on the crop root rot, and compared with agent with single biocontrol fungi it has a lot advantages such as wide control scope and complementary role. The granules agent is proper for controlling root rot of plants, crops, trees and flowers, and it is also has promoting effect on crops, meanwhile it is right for people and livestock and crop safety, with advantages such as long-term action, no pollution to the environment, easy application and low-cost.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
Test method for inhibiting Phytophthora nicotianae
InactiveCN110229868ABest antibacterial rateImprove stabilityMicrobiological testing/measurementBiological material analysisSnow moldNicotiana tabacum
The invention relates to a test method for inhibiting Phytophthora nicotianae. A plate dilution coating method is adopted to separate rhizosphere soil microorganisms of healthy tobacco plants in the tobacco field with the tobacco black shank disease, and two antagonistic bacteria and three Trichoderma spp. are screened out from Phytophthora nicotianae by a plate confrontation test; and the numberof two antagonistic bacteria is B1 and B2 respectively, and the single-bacterium bacteriostatic rates of both of the bacteria are more than 80 percent, wherein the inhibition rate of the B1 to Phytophthora nicotianae is more than 90 percent, and both of the bacteria are identified to be paenibacillus polymyxa; the number of the three Trichoderma strains are YCS-Tr1, YCS-Tr2 and YCS-Tr3 respectively, the single-bacterium bacteriostatic rates of the strains are more that 94%, and the three strains are identified as green Trichoderma spp., Trichoderma paraviridescens and Trichoderma koningiopsisrespectively. The test data analysis obtains the strain combination mode with the optimal bacteriostatic rate, is beneficial to more accurately implementing the prevention and treatment work of inhibiting the Phytophthora nicotianae in the later field application, and provides a reference test method for acquiring a composition for efficiently and stably inhibiting the Phytophthora nicotianae.
Owner:TOBACCO RES INST HENAN ACADEMY OF AGRI SCI
Pseudo-ginseng continuous cropping soil biocontrol microbe safely adding method
InactiveCN106376373AImprove germination rateReduce usageBiocideDead animal preservationContinuous croppingSnow mold
The invention discloses a pseudo-ginseng continuous cropping soil biocontrol microbe safely adding method; the method uses microbe inocula to carry out hole application treatment before a pseudo-ginseng seed strip is transplanted, and / or to carry out fertigation treatment after the pseudo-ginseng seed strip is transplanted, wherein the microbe inocula refers to Bacillus spp and / or Trichoderma spp. The biocontrol microbe safely adding method can effectively control pseudo-ginseng diseases, and can effectively improve soil conditions especially on a pseudo-ginseng continuous cropping obstacle land, thus alleviating continuous cropping obstacles, improving pseudo-ginseng emergence rate and survival rate, and improving soil usage efficiency.
Owner:文山苗乡三七科技有限公司
SCAR marker of biocontrol Hypocrea virens, its application and quantitative detection method
Belonging to the technical field of bioengineering, the invention relates to an SCAR (sequence-characterized amplified region) marker of biocontrol Hypocrea virens, its application and quantitative detection method. The sequence of the marker is shown as SEQ ID NO:1. According to the invention, an RAPD (random amplified polymorphic DNA) technology is employed to analyze the Hypocrea virens SS161 and 12 strains of the same species and genus to successfully screen a specific fragment of the Hypocrea virens SS161, after recovery and sequencing of the specific fragment, the SCAR marker of the Hypocrea virens SS161 can be obtained by cloning. Fluorescent quantitative PCR primers and a TaqMan probe can be designed based on the sequence, and by means of continuous optimization, the real-time quantitative PCR detection method of the biocontrol Hypocrea virens SS161 is successfully established and developed. The quantitative detection method provided in the invention can be used for identification and tracking detection of the biocontrol Hypocrea virens SS161. The invention provides a simple and efficient method for studying the ecological adaptability of Trichoderma spp. in soil and other natural environments and judging whether trichoderma biocontrol bacteria are applicable in actual production. The method has important studying and application value.
Owner:CHINA NAT RICE RES INST
Trichoderma SPP strains with high capactiy of fungus biological control and selection process thereof by molecular markers
ActiveUS20060034806A1Large capacityGood biological controlBiocideFungiOrganismDrug biological activity
Owner:COSMOCEL
Application method of medicine for treating melon leaf downy mildew
Owner:QINGDAO DONGYI JINHE AGRI TECH
High-yield IAA Trichoderma viride engineering bacterial strain, and construction method and application thereof
ActiveCN112553230AImprove the ability to produce IAAStrong resilienceBiocideFungiBiotechnologyTrichoderma sp.
Owner:BIOLOGY INST OF SHANDONG ACAD OF SCI +1
Preparation method of seaweed fertilizer degraded by mixed microorganisms
InactiveCN106278572AAvoid mutual inhibitionPromote decompositionBio-organic fraction processingBiological combination treatmentFiltrationPseudomonas fulva
The invention discloses a preparation method of a seaweed fertilizer degraded by mixed microorganisms. The preparation method comprises the following steps of performing cutting up, performing homogenating, performing microbial degradation, performing filtration, performing initial inspection, performing sterilization, and performing rechecking so as to prepare finished products, wherein the step of microbial degradation is performed through the following steps in sequence: firstly, adding trichoderma spp, then adding streptomycesgriseus and pseudomonad. According to the preparation method disclosed by the invention, the mixed microorganisms of the trichoderma spp, the streptomycesgriseus and the pseudomonad are added, and homogenated phaeophyceae is subjected to temperature-controlled aerobic fermentation, so that microorganism strains can be sufficiently cultivated, rapid degradation is realized, strain dosage is reduced, the cost is reduced, and the yield is increased. The prepared seaweed fertilizer finished products have the function of replacing a pesticide for controlling mycotic plant diseases, and particularly have good control effects on brown rots of peach trees.
Owner:NINGBO AVANUOV BIOTECH CO LTD
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