64 results about "Trichoderma spp" patented technology

A slow-release solid chemical composition for environmental bioremediation is provided. The composition comprises a source of soluble organic substrates which include sugars, soluble organic polymers and mixtures of them in an amount of 7% to 90%, insoluble organic substrates an amount of 10% to 70%, complex inorganic phosphates in an amount of 0.5% to 7% and soluble organic salts in an amount of 2% to 70%. The insoluble organic substrates include fibrous plant materials, starches, cellulosic materials and mixtures of these substrates. The complex inorganic phosphates include ringed metaphosphates, linear polyphosphates and mixtures. The organic salts include lactates, formates, acetates, citrates, etc. Also the composition further comprises microorganisms which include Bacillus spp., Rhizobium spp., Bradyrhibzobium spp., Fibrobacter spp., Clostridium spp., Pseudomonas. spp., Geobacter spp., Arthrobacter spp., Nocardia, spp., aspergillus spp., Trichoderma spp., Candida spp., Yarrowia spp. and combinations of these microorganisms. The composition can be prepared in various forms, including granules, briquettes, pellets, tablets or capsules.

A trichoderma spp chlamydospore production method of obtaining trichoderma spp from crop root surface soil is disclosed. The method obtains chlamydospore through controlling nutrition condition , adding chlamydospore promotor, regulating other technical parameter to control the fermentation condition. First the trichoderma spp from corp root is purified and then transferred to tube slant as stock bacteria, then vibrated in triangular tube to become bacteria liquid, at last fermented in fermenter to produce chlamydospore suspension liquid. The merit of inventive chlamydospore is long life.

The invention provides a preparation method of a microbial organic fertilizer. The method comprises the following steps: (1) chlamydospore preparation by carrying out submerged fermentation on Trichoderma harzianum strains with preservation number of CGMCC NO:5812 to produce chlamydospores; (2) manure particle preparation by mixing manure, humic acid and water, and inoculating deodorizing bacteria for fermentation deodorization and granulating and drying to obtain the manure particles; and (3) mixing the manure particles and the chlamydospores. The invention also provides a microbial organic fertilizer obtained from the above preparation method. The invention also provides purpose of the microbial organic fertilizer in control of plant diseases. Through the above technical scheme, the invention can significantly improve the disease control effect of Trichoderma spp.

The invention relates to a Trichoderma strain for antagonizing soil-borne disease, belonging to the field of biological control. The Trichoderma strain is Trichoderma asperellum ZJSX5003 CGMCC No.6480. The Trichoderma strain provided by the invention has the characteristics of high growth speed and high spore production amount; the in-vitro standoff pathogen inhibition rates are respectively up to 79.79%, 73.68% and 72.25%, the pathogen spore survival rates are respectively 0.00%, the in-vivo preventive effects for cucumber fusarium wilt and maize stem rot are respectively 85.08% and 66.67%, the enzyme activity of chitinase is 4.0783U, the enzyme activity of beta-1,3-glucanase is 0.8050U, the enzyme activity of extracellular proteinase is 4.8503U, and the content of silicone, terpene and carboxylic acid having antagonistic actions in antibiotic secondary metabolites is 44.44%. The Trichoderma strain provided by the invention has the advantages of high antagonism and high specificity. The biological pesticide produced from the strain can effectively control the soil-borne disease, thereby ensuring the stable increase of crop and vegetable yields.

The invention discloses a botanical bactericidal preparation. The botanical bactericidal preparation comprises the following components, by weight, 0.1-50% of thymol, 5-30% of an emulsifier, 0.1-20% of azone, 10-60% of ethanol, and 0.1-84.8% of water, and 1ml of the botanical bactericidal preparation comprises Trichoderma spp with the number of 1*10<7-9>. The invention also discloses a preparation method of the botanical bactericidal preparation and an application of the botanical bactericidal preparation in the blight control. The thymol-based botanical bactericidal preparation of the invention, which has effective components from plants with the insecticidal function or extracts from the plants, allows the blight of crops to be effectively controlled, and has the multiple efficacies of crop growth promotion, yield and income increase, quality improvement, nutrient utilization rate improvement, pest control, no pollution, and the like, is suitable for the production of nuisance-free,green and organic agricultural products.

The invention relates to a Trichoderma strain for antagonizing maize stem rot and banded sclerotial blight and application thereof, belonging to the field of biological control. The Trichoderma strain is Trichoderma atroviride SG3403 CGMCC No.6479. The invention also relates to application of the Trichoderma strain in preparing pesticides for controlling maize stem rot and banded sclerotial blight. The Trichoderma strain provided by the invention has the advantages of high growth speed and high spore production amount, the in-vitro standoff pathogen inhibition rates are respectively up to 71.91%, 74.77% and 73.11%, the enzyme activity of chitinase is 4.1452U, the enzyme activity of beta-1,3-glucanase is 0.5969U, and the enzyme activity of extracellular proteinase is 2.4545U. The strain provided by the invention can be used for producing biological pesticides for efficiently and quickly controlling maize stem rot and banded sclerotial blight.

The invention discloses a production process for fermenting trichoderma spp by taking orange peels as raw materials, which belongs to the technical field of microorganisms. The production process comprises the following steps: (1) preprocessing: drying, grinding and sieving the orange peels; (2) mixing preprocessed orange peel powder with wheat bran and de-ionized water according to a certain ratio, and sterilizing by high-pressure steam; (3) inoculating trichoderma onto a sterilized material for solid-state fermentation; (4) drying a solid fermentation product to obtain a trichoderma preparation product. The trichoderma spp preparation produced by the process has capabilities of promoting plant growth and inducing plants to resist adversity and diseases, can be used for preventing and treating soil-borne diseases and promoting repair of plants in polluted soil, and provides a new way for resource utilization of the orange peels.

The invention relates to a fertilizer synergist and a preparation method thereof. The fertilizer synergist contains, in percent by weight, 60-65% of humic acid, 25-30% of trace elements, and 5-15% of composite bacterial strains. The trace element composition is composed of the following raw materials in percent by weight based on the total weight of the race element composition: 10-20% of calcium sulfate, 10-20% of magnesium sulfate, 0.5-1.5% of borax, 2.5-4.5% of ferrous sulphate, 0.5-1.5% of manganese sulfate, 0.5-1.5% of zinc sulfate, 0.25-0.75% of copper sulphate, and 0.25-0.75% of ammonium molybdate. The composite bacterial strains is composed of the following raw materials in percent by weight based on the total weight of the composite bacterial strains: 30% of azotobacter, 25% of a phosphate-dissolving bacterium, 10% of actinomyces, 10% of potassium bacteria, 12% of ferment bacteria, and 13% of trichoderma spp. The fertilizer synergist is capable of obviously improving fertilizer utilization rate and absorption rate and improving soil fertility, and helps to substantially increase crop yield and improve economic benefit.

The invention discloses a making method of candied Chinese chestnuts. The making method comprises the following steps of (1) pretreating raw materials: removing shells from Chinese chestnuts, stripping out coats, and boiling the treated Chinese chestnuts with water for 3-5min; (2) performing color protection: immersing the boiled Chinese chestnuts in a color protecting liquid containing a chestnutshell extract, wherein in the color protecting liquid, the percentage by mass of a Chinese chestnut extract is 20-25%; (3) performing enzyme treatment: placing the Chinese chestnuts obtained in step(2) in enzymatic hydrolysate containing amylases and a trichoderma spp fermented product for enzymolysis; (4) performing treatment in a sugar solution: placing the Chinese chestnuts after enzyme treatment in the sugar solution, performing sugaring with microwaves for 30min, and the sugar solution consists of the following raw materials in percentage by mass: 10-20% of glucose, 5-10% of a persimmoncolloid extract, and the balance of water; and (5) performing drying: drying the Chinese chestnuts treated in the sugar solution through microwave drying until the water content is 8-12%. The made candied Chinese chestnuts are good in shaping, not liable to break, low in hardness and good in texture structure.

The invention relates to a production method of a trichoderma spp. biocontrol agent, and belongs to technical fields of microbial fertilizers and the resource utilization of waste water. According to the method, grape peel dregs and grain stillages produced during grape wine production are used as raw materials for the production, and a method of solid state fermentation is adopted to produce the trichoderma spp. biocontrol agent. The production process comprises: (1) taking waste water of kitchen garbage as a medium to inoculate trichoderma spp. and preparing liquid seeds by liquid culture; (2) inoculating the liquid seeds to prepare a large number of liquid trichoderma spp. products with waste water of kitchen garbage as raw materials for the fermentation; (3) adding the cultured liquid seeds or the liquid trichoderma spp. products into biomass materials, and performing solid state fermentation to prepare solid trichoderma spp. products; (4) drying the fermented products, adding stabilizing agents and auxiliary agents, pulverizing, and packaging. The process for producing trichoderma spp. microbial fertilizers has low production cost and is environment-friendly; the products can significantly improve the quality of soil environment, increase crop yields, and control various plant diseases caused by fungi. A processing method for effectively processing plenty of high concentration waste water produced during the production of kitchen garbage is simultaneously realized and environmental pollution is reduced.

The invention relates to extracellular tyrosinases obtainable from Trichoderma spp. and to methods and means for producing them by recombinant technology. The enzymes are particularly useful in cross-linking food proteins.

Trichoderma spp. strains with high capacity for fungus biological control in wide ranges of temperature and pH are described, such strains being compatible with each other. Likewise, a process of selection of such strains through molecular markers is described. The described process reduce the number of necessary experiments to determine if a Trichoderma strain, not previously described, can display a biological activity more acceptable than those well known.

The invention provides a high-yield IAA Trichoderma viride engineering bacterial strain, and a construction method and application thereof, and belongs to the technical field of microorganisms. Encoding genes which independently encode tryptophane synthase and tryptophane decarboxylase are authenticated from the genome of the (Trichoderma viride) Tv-1511, and Trichoderma viride engineering bacteria (TvTRPS-OE, TvTDC-OE and TvTRPS / TDC-OE) subjected to TvTRPS and / or TvTDC overexpression are successfully constructed. An experiment proves that compared with an original Trichoderma spp. bacterialstrain, the IAA production capacity of the constructed three types of Trichoderma viride engineering bacteria is obviously enhanced, meanwhile, the three types of Trichoderma viride engineering bacteria have a higher stress resistance and plant growth promotion capacity, application of the Trichoderma spp. bacteria in industrial production can be accelerated, and therefore, the three types of Trichoderma viride engineering bacteria have a good practical application value.