1984 results about "Root rot" patented technology

The invention provides bacillus amyloliquefaciens K-8 and bactericide thereof. The preservation number of the bacillus amyloliquefaciens K-8 strain is CGMCCNO.6486. The bactericide takes the bacillus amyloliquefaciens K-8 strain as an active ingredient. The bacillus amyloliquefaciens K-8 strain is cultured to obtain a liquid bactericide, and a solid bactericide can be obtained by adding a carrier or mixture of the carrier and auxiliaries into the liquid bactericide. The bacillus amyloliquefaciens K-8 strain and the bactericide thereof provided by the invention can be used for effectively preventing and treating diseases such as bacterial wilt, wilt, angular spot, banded sclerotial blight, rice false smut, soft rot, rot, canker and root rot. The bactericide is simple to prepare and easy to use, is applicable to industrialized production and has wide application prospect.

Fungal/bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal/bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal/bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

The invention relates to a vegetable planting method. The method comprises a seed treating step, a seedbed seedling raising step, a fertilizer applying and uniform plowing step, a ridging and ditch feeding step, a bare root planting step, a black plastic film covering step and a field management step, wherein the seed treating step comprises the following specific steps of: irradiating seeds with ultraviolet rays for 10 to 45 minutes to sterilize the seeds; soaking the sterilized seeds in solution of potassium permanganate in a ratio of potassium permanganate to water of 1:500 for 10 minutes; cleaning the soaked seeds with water; and soaking the cleaned seeds in 10 percent solution of trisodium phosphate for 5 to 10 minutes for disinfecting; and mixing the soaked seeds by using lactobacillus. No chemical fertilizer or pesticide is used in a planting process, so that the produced vegetables are not polluted. Actinomycetes and the lactobacillus are used in the seedbed seedling raising step, the fertilizer applying and uniform plowing step and the field management step, so that vegetable root rotting can be prevented and the infestation of plant diseases and insect pests to root systems is reduced.

The invention relates to an application method of shenqinmycin in controlling crop diseases and a product of shenqinmycin. Shenqinmycin has the advantage of substantially improving the root rot and basal decay capability of crops. The application method concretely comprises the following steps: uniformly mixing shenqinmycin original drug with a nitrogenous fertilizer, a phosphate fertilizer and a potash fertilizer to obtain a mixer; and applying the mixer to the crops. The prouct has protruding control effects on crop root rot and basal decay, and the effective rate improves by more than 10% than the effective rate of individually applying shenqinmycin. The product has a wide purpose in agriculture, allows problems of the crop root rot and basal decay caused by planting same crops all year round to be solved, and has substantial economic benefits and environmental protection benefits.

The invention provides a liquid biological organic fertilizer with high content of organic substances and a preparation method. The fertilizer is characterized by comprising the following components by weight: 60% of a humic acid release inhibitor, 0.4% of a composite biological agent, 10% of fulvic acid potassium, 12% of soluble nitrogen-phosphorus-potassium raw materials, 9% of a chelating medium trace element agent, 8.47% of a soil conditioner, 0.93% of compound sodium nitrophenolate and 0.1% of naphthalene acetic acid; and the fertilizer is prepared by mixing, heating, chelating, concentrating, cooling, microorganism adding, filtering and residue removing. The invention has the following beneficial effects: plant nutrition is directly supplemented; the contents of absorbable organic substances for a root system in soil are rapidly supplemented; growth of the root system is rapidly promoted; absorption area of the root system is enlarged; the chelating medium trace element agent can not be fixed by the soil and exists in a free state in the soil, so the chelating medium trace element agent is easy to be captured and absorbed by the root system in the soil; physiological diseases and pests are prevented from occurrence; quality of an agricultural product is improved; root-rot disease and stem-rot disease are prevented from occurrence; occurrences of fusarium and verticillium wilts and bacterial wilt are reduced; meanwhile, the fertilizer is capable of resisting salinity and alkalinity, resisting continuous cropping diseases, and killing soil-borne diseases.

The invention provides bacillus amyloliquefaciens SZ-60 which is characterized in that the collection number is CGMCC No.8277. The bacterial colony formed by the strain on a beef-extract peptone (NB) medium by virtue of single-cell reproduction is irregular with oyster white color, slight upheaval at the center and wet and semitransparent surface; the microscopy is of a rod shape, and the size is (0.3-0.4)*(3.2-3.3)microns; with flagella, G- and spores, the strain can grow on the beef-extract peptone (NB) medium containing 2-5% of NaCl; the formula of the beef-extract peptone (NB) medium contains 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 17g of agar and 1,000ml of water, and the pH is 6.8-7.2. The bacillus amyloliquefaciens realizes a remarkable antagonistic action on the main pathogenic bacteria causing ginseng root rot, epidemic diseases, sclerotinia sclerotiorum, cylindrocarpon destructans, black spot and damping off. The invention also provides an application of the bacillus amyloliquefaciens SZ-60 in preventing plant fungal diseases, or application in preparing a microbial preparation for preventing plant fungal diseases.

The invention discloses application of streptomyces griseoflavus in biological prevention and control of plant diseases, particularly streptomyces griseoflavus NMG6-3-9 CGMCC No. 3441 in biological prevention and control of plant diseases. The provided strains have good antagonism effect on pathogenic bacteria of alfalfa root rot, can be used for the biological prevention and control of alfalfa root rot and other plant diseases, and have wide application potential in the field of biological prevention and control of plant diseases. The invention lays a foundation for the biological prevention and control of alfalfa root rot and further provides a scientific basis for the development and the application of a biological prevention actinomycete preparation.

The invention relates to application and preparation of bacillus amyloliquefaciens subsp. plantarum and a bacterial agent thereof. An XH-9 bacterial strain is identified as bacillus amyloliquefaciens subsp. plantarum according to mycelial morphology, colony characteristics, physiological and biochemical indexes and 16S rDNA sequence analysis and is preserved in the China General Microbiological Culture Collection Center, and a preservation number is CGMCC NO.13151. The strain is high in antagonistic effect on pathogenic fungi such as fusarium oxysporum, bipolaris sorokiniana, fusarium pseudograminearum, colletotrichum gloeosporioides, botryosphaeria dothidea and alternaria alternata and is capable of generating heteroauxin and 1-aminocyclopropanecarboxylic acid (ACC) deaminase to stimulate plant growth and capable of degrading celluloses and can be stably colonized at rhizospheres of crops such as wheat, corn, peppers and the like. The bacterial agent prepared from the bacterial strain can be applied to prevention and treatment of root rot diseases or other soil-borne fungal diseases of crops such as wheat, corn, peppers and the like and has dual functions of disease prevention and growth promotion. The bacterial agent is simple in preparation process, short in fermentation period, low in cost and beneficial to industrial production and transport.

The invention discloses bacillus subtilis CYY-25 and belongs to the field of microorganisms. The bacillus subtilis CYY-25 is preserved with the preservation number CGMCC No.8995 in China General Microbiological Culture Collection Center and is capable of producing cellulase when salinity ranges from 0% to 15%. The bacillus subtilis CYY-25 is capable of synthetizing IAA and siderophore, dissolving sparingly soluble inorganic phosphorus, promoting plant growth and well inhibiting multiple kinds of plant pathogenic fungi such as fusarium oxysporum, pyricularia oryzae, sclerotinia sclerotiorum, fusarium moniliforme and sclerotinia sclerotiorum, is of great significance to prevention and control of alfalfa root rot and is suitable for being developed and used as an agricultural fungicide.

The invention relates to a bacillus subtilis preparation for preventing and controlling soil-borne diseases, as well as a preparation method thereof. The bacillus subtilis preparation is prepared by taking the thalli and metabolites of bacillus subtilis B579 with preservation number of CGMCC No.2270 as an effective active constituent which are matched with agriculturally acceptable carrier and a heat-resistant protective agent; the thalli content of the effective active constituent is 8 to 80 billion thalli per gram; and the mass content of the heat-resistant protective agent is 3 to 5 percent. The bacillus subtilis preparation can effectively prevent and control cucumber drooping diseases, cucumber wilt, eggplant root rot, eggplant damping-off, pepper blight, tomato basal stalk rot, grape botrytis cinerea and banana anthracnose. The bacillus subtilis preparation has a control efficiency more than 70 percent, has obvious effect of promoting plant growth, and is characterized in high efficiency, wide antimicrobial spectrum, simple use, low cost, no environmental pollution and improvement on the micro-ecological environment of soil.

The invention provides a composite microbial inoculant for controlling plant continuous-cropping diseases, which comprises trichoderma harzianum, bacillus subtilis, and gliocladium roseum, and also comprises phosphate-solubilizing bacteria and potassium-solubilizing bacteria; the accession number of the trichoderma harzianum strain is CGMCC NO. 7861; the accession number of the bacillus subtilis strain is CGMCC NO. 7850; the accession number of the gliocladium roseum strain is CGMCC NO. 9008. The invention also provides a preparation method and an application of the composite microbial inoculant. Through the selection of high-efficient bacterial strains such as disease-preventing trichoderma, bacillus subtilis, gliocladium roseum, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, the composite microbial inoculant of the invention promotes the growth of crops, improves the utilization rate of fertilizers, enhances the stress resistance such as disease resistance and the like, improves the quality of crops, and can control diseases such as root rot, verticillium wilt, damping off, botrytis, epidemic diseases, powdery mildew, sclerotinia rot, seedling-stage damping off, and the like.

The invention discloses a production method for an organic fertilizer. The production method can be used for mixing excrements of herbivore animals and plasters or plant ashes and coal ashes which are obtained by combusting lime sludge of a sugar factory or manioc waste and bagasse of a starch factory and quickly converting organic waste into available organic fertilizers by a zymophyte ecological method. According to the method provided by the invention, the stack fertilizer preparing time can be shortened, and the labor and the time can be saved. By using the organic fertilizer produced by the method, a perfect root environment can be constructed, so that rot diseases can be avoided; plants can fully realize the functions of absorbing the fertilizers, and crops can quickly grow up; the physical property and the chemical property as well as the microbial state of the soil are thoroughly balanced, so that the soil can be forever kept in an optical state for culturing crops. The organic fertilizer produced by the invention can be mixed with other trace elements, a plant growth promoter, chemical fertilizers and the like to prepare a composite fertilizer. Beneficial microflora can be added for further treatment; the non-toxic and harmless microorganism fertilizer can be compounded.

The invention discloses a compound microbial fertilizer for antagonism of soil-borne fungal diseases, and a preparation method and an application thereof; the fertilizer takes humic acid as a compound carrier, includes bacillus amyloliquefaciens HFJ-7 with the bacteria content of 1.8*10<8>-2.1*10<8> CFU/g, and also includes bacillus megatherium B3. The bacillus amyloliquefaciens HFJ-7 in the compound microbial fertilizer has the preservation number of CGMCC No.10011. The bacillus amyloliquefaciens HFJ-7 strain can be antagonistic to rhizoctonia solani, fusarium oxysporum, root rot pathogen, cucumber fusarium wilt pathogen, phytophthora capsici leon pathogen and other soil-borne disease pathogenic fungi. After the bacillus amyloliquefaciens HFJ-7 is compounded with the bacillus megatherium B3, the resistance to rhizoctonia solani and pepper root rot pathogen can be significantly improved, a disease state index of pepper seedling root rot disease can be significantly reduced, and the occurrence and spreading of pepper root rot can be effectively prevented and controlled.

The invention discloses a cultivation method of Dendrobium officinale. Natural growth environment for Dendrobium officinale is simulated successfully, a cultivation bed is made of cement pantiles, substrate is made of pine bark, self-made nutrient solution is used as fertilizer, and accordingly supply of water and fertilizer is guaranteed, root rotting caused by waterlogging and damage of insects, rats, weeds and diseases are avoided, transplant survival rate of Dendrobium officinale seedlings reaches more than 98%, Dendrobium officinale grows fast and blossoms or grow stems and leaves, one to three years early, yield is high (yield per unit area is more than 250kg), content of effective ingredients is high, quality is good, and the problems of low transplant survival rate, growth slowness, low yield and high cultivation cost of Dendrobium officinale seedlings are solved. The cultivation method includes the steps of making the cultivation bed, making the culture substrate, fixation and distribution of the cultivation bed, planting Dendrobium officinale, management of water and fertilizer, insect damage control and harvesting.

The invention provides bacillus methylotrophicus SZ-3 and SZ-81, with collection numbers of CGMCC No.8275 and CGMCC No.8276 respectively. The bacillus methylotrophicus SZ-3 has obvious antagonistic effects on pathogenic bacteria causing root rot, black spot and sclerotinia sclerotiorum of ginseng. The bacillus methylotrophicus SZ-81 has obvious antagonistic effects on pathogenic bacteria causing epidemic diseases, root rust rot and black spot of panax ginseng. The invention also provides an application of the bacillus methylotrophicus SZ-3 and SZ-81 to control of fungal diseases of plants or an application to preparation of microbial preparations for controlling fungal diseases of plants.

The invention discloses a streptomyces microflavus. The streptomyces microflavus is the streptomyces microflavus NMG2-4-8, and the preservation number thereof is CGMCG No.3442. The streptomyces microflavus is separated and screened from alfalfa healthy tree rhizosphere soil for the first time in the invention, and experiments prove that the streptomyces microflavus has remarkable antagonistic action for 18 pathogenic fungi and 9 bacteria and broad antibacterial spectrum. The streptomyces microflavus has strong inhabiting action for crop pathogenic bacteria, such as alfalfa downy mildew and the like, shows a good disease prevention effect for the alfalfa downy mildew, powdery mildew, brown spots, stemphylium leaf spot disease, root rot disease and the like, and indicates that the bacterial strain has broad application prospect in the biological prevention and cure fields of alfalfa diseases.

The invention discloses bacillus velezensis and an application thereof to preventing and treating tomato phytophthora root rot. The invention provides bacillus velezensis (bacillus velezensis) ZF50, and the preservation number of the bacillus velezensis in the China General Microbiological Culture Collection Center is CMCC No. 16775. The invention provides an efficient biocontrol strain for preventing and treating the tomato phytophthora root rot, and bacillus velezensis has important production significance for a green prevention and control technique of tomato diseases and promotion of sustainable development of the tomato industry.

The invention discloses a special-purpose seedling-raising controlled-release fertilizer for a tobacco seedling-raising plate and a preparation method of the fertilizer, belongs to the technical field of pesticides and solves problem that the existing fertilizer is low in utilization rate and cannot prevent tobacco diseases and insect pests. A weight ratio of N to P to K in a film-coating controlled-release fertilizer is (15-20):(4-10):(6-15); the film-coating controlled-release fertilizer is further added with 0.1%-0.5% of fungicide, wherein the fungicide is prepared from the following materials: tobacco, black false hellebore, garlic, pyrethrum, ailanthus, mandala, uniflower swisscentaury root and weeping willow. The controlled-release fertilizer disclosed by the invention has a long release period, realizes satisfying demands of crops on nutrients such as nitrogen, phosphor, potassium, calcium, sulfur and the like, in the whole growth period by single-dose application. Meanwhile, an occurrence rate of diseases such as tobacco bacterial wilt, black shank and root rot, is greatly reduced, infection of germs in a seedling-raising period is avoided, and occurrence of diseases of stalks which are transplanted in a large field is greatly reduced. And meanwhile, the fertilizer is prevented from adding for many times, and labor cost is greatly lowered.

The invention provides a nutrient fertilizer for fuchsia. The nutrient fertilizer can meet the basic nutrient requirements of the whole growth period of the fuchsia, and is favorable for promoting growth and development of roots and leaves of the fuchsia. The fertilizer has sterilization, pest killing and pest and disease prevention while being applied, and has a good effect of preventing, controlling or relieving aphids, blight, rust disease, root rot, powdery mildew and the like possibly produced in the fuchsia. After the fertilizer is applied, the fuchsia has the advantages of large and multiple flower buds, bright color, few pest and disease and quick growth and development speed and also has high ornamental value.

The invention discloses a planting method for regulating and controlling continuous cropping obstacles of atractylides macrocephala koidz, which comprising the following steps of: applying 40 to 100g of lime nitrogen to each square meter of a continuous cropping field, performing deep tillage, watering soil by using 500 to 1,000 times of prochloraz or fluazinam, covering by using a thin film for 15 to 20 days, uncovering the thin film, ventilating for 10 to 15 days, and planting the atractylides macrocephala koidz and applying 0.5 to 3.5kg of biological organic fertilizer per 5 square meters; and irrigating the root of the atractylides macrocephala koidz once by using 500 to 1,000 times of the prochloraz or the fluazinam or hymexazol before disease outbreak in June and July; or the planting method comprises the following steps of: ploughing and covering the thin film after the crop rotation of spring corn, irrigating until water is above the soil surface, keeping hermetic for 20 to 90 days, and uncovering the thin film to plant the atractylides macrocephala koidz. By the planting method, the incidence rate of diseases such as root rot, southern blight and the like of the atractylides macrocephala koidz is reduced greatly, the seedling survival rate of the atractylides macrocephala koidz is improved, and the yield of atractylides macrocephala koidz rhizomes is improved. Simultaneously, the utilization rate of land can be improved to improve economic benefits.

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.