98 results about "Endophyte" patented technology

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

The present invention relates to the delivery of a methane inhibitor to a plant or a grazing animal. This is achieved by infecting grass with an endophyte which has been modified to produce the methane inhibitor in planta. The endophyte produces the methane inhibitor in planta, which may be consumed by the grazing animal.

The invention belongs to the technical field of microorganisms, and particularly relates to a kelp endophyte, i.e., presteria megaterium H403 and an application of the kelp endophyte, i.e., presteria megaterium H403. In order to solve the problems that the kelp seedling raising period is long and the cost is high, an endophytic bacterium is separated from kelp and is named as presteria megaterium H403, a co-culture experiment shows that the strain can remarkably improve the germination rate and the growth rate of kelp seedlings, and the length of kelp in a treatment group added with 14 * 10 < 6 > cfu bacterial liquid is 3.6 times that of a control group. The invention discloses a molecular mechanism for promoting the growth of the kelp through multiple ways by virtue of the Pristeria megaterium H403, a theoretical basis is provided for developing a microorganism-assisted seedling raising technology, the kelp culture cost is expected to be reduced, and the sustainable development of the industry is promoted.

The application discloses application of a bacteria-sugar composition in stress resistance regulation of saline-alkali soil and belongs to the technical field of plant growth regulation. The microorganism is Bacillus, and the plant source extract is a salvia miltiorrhiza-safflower composite polysaccharide. The biological preservation number of the Bacillus in the composition is CGMCC No. 25566, the bacterial concentration is 8.5*10 5 CFU / mL, and the salvia miltiorrhiza-safflower composite polysaccharide is prepared by mixing salvia miltiorrhiza and safflower as raw materials according to a mass ratio of (10~1):(1~10) and adopting polysaccharide extraction, and the concentration is 1 ug / mL. The Bacillus with the biological preservation number of CGMCC No. 25566, which is screened from medicinal plant salvia miltiorrhiza symbiotic microorganism, is combined with the salvia miltiorrhiza-safflower composite polysaccharide to obtain a bacteria-sugar composition for regulating crop tolerance to moderate to severe saline-alkali soil, and the application of salvia miltiorrhiza endophyte and plant source polysaccharide is further developed.

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of Erwinia gerundensis which provides bioprotection and / or biofertilizer phenotypes to plants into which it is inoculated. The present invention also discloses plants infected with the endophyte and related methods.

The present invention relates to a composition comprising:an isolated endophyte, wherein the endophyte is a strain of Priestia aryabhattai, andan adjuvantwherein the Priestia aryabhattai strain has Accession Number DSM 34352.

The application discloses a new antibacterial compound and a preparation method and application thereof, and the new antibacterial compound is obtained by fermenting endophytic fungi (Epicoccum latusicollum) under stress of pathogenic fungi (Fusarium oxysporum), filtering, discarding mycelia, enriching the fermentation liquor by using a macroporous adsorption resin, and adopting high performance liquid preparation separation, and the chemical name of the new compound is (6E,8E)-13-acetoxy-3-hydroxy-8,10,12,14-tetramethylhexadeca-6,8-dienoic acid. The new compound obtained by fermenting endophytic fungi under stress of pathogenic fungi has significant antibacterial activity, and the application provides a scientific basis for mining endophytic fungi antibacterial natural compounds based on stress effects.

The invention relates to the technical field of microorganisms, in particular to a desert plant endophyte, a microbial agent and application, the desert plant endophyte is Pristeria aryabhattai HB-4 and is preserved in the China Center for Type Culture Collection on November 17, 2025, and the preservation number of the desert plant endophyte is CGMCC No.36665. The invention further relates to a method for preparing the desert plant endophyte. The strain can efficiently produce indoleacetic acid and has remarkable growth promoting and stress resistance functions. After the strain is inoculated, the fresh weight, the dry weight, the stem length, the chlorophyll content and the catalase activity of wheat are remarkably improved, and the malondialdehyde content is reduced. The invention further provides a microbial agent containing the strain and application of the microbial agent in the aspects of promoting plant growth and improving stress resistance, the microbial agent is particularly suitable for cultivation of crops such as wheat in drought and barren environments, and a new strain resource is provided for development of agricultural microbial preparations.

The application discloses a plough head mold GXU-F34 for promoting linaloe, and the plough head mold GXU-F34 is obtained by purifying and separating samples cut from old trees and can be used for promoting linaloe. The plough head mold GXU-F34 is a plant endophyte, can continuously produce some enzymes to stimulate the defense system of Aquilaria plants, and a series of enzymes produced by saprophytic fungi microorganisms play an important role in the defense mechanism of Aquilaria plants, and finally lead to the production of linaloe.

The invention discloses an amorpha fruticosa aerial seeding seed pelleting treatment method and equipment for increasing the germination rate, and particularly relates to the field of forest tree seed treatment.The amorpha fruticosa aerial seeding seed pelleting treatment method.The amorpha fruticosa aerial seeding seed pelleting treatment method comprises the steps that S1, amorpha fruticosa seeds are cleaned, disinfected and soaked, washed with clear water after soaking is completed, and then naturally aired; s2, collecting a fresh koelreuteria paniculata seed sample to prepare an endophyte solution, preparing an adhesive, and uniformly stirring the adhesive to fully dissolve; s3, adding the amorpha fruticosa seeds and an adhesive into a stirrer, uniformly stirring, then adding the endophyte liquid, continuously stirring, then selecting medical stone and bentonite as carriers, and quickly and uniformly wrapping the seeds to obtain a mixture; and step S4, mixing the mixture, and then carrying out granulation treatment. Through the synergistic effect of multi-step pretreatment and the pelleting process, the germination rate and the survival rate of the amorpha fruticosa seeds are remarkably improved, the physical characteristics of the seeds are optimized through pelleting treatment, and the aerial seeding adaptability is greatly improved.

The invention relates to the technical field of green prevention and control of agricultural diseases and insect pests, and discloses a green prevention and control method for agricultural planting diseases and insect pests, a composition takes a core preform carrying negative charges as a core and takes functional chitosan carrying positive charges as a shell, and a core-shell structure is formed through electrostatic self-assembly. The core preform comprises an endophyte active matter, an anionic inner shell skeleton polymer and chelated medium and trace nutrient elements; the functional chitosan is prepared by grafting signal oligosaccharide and an environmental response group on a chitosan molecular chain. According to the composition disclosed by the invention, an endophyte active matter is effectively isolated through a core-shell structure, the storage stability of the composition is improved, and slow release is realized; meanwhile, the direct biocontrol effect of the active matter, the plant immunity induction effect of the functional chitosan and the nutritional supplement effect of the medium and trace nutrient elements are organically combined, multi-mechanism synergistic interaction is achieved, and the field control effect is excellent.

Endophyte combinations, compositions, and methods of using the endophyte combinations and compositions for improving plant and algae productivity and tolerance to environmental stresses.

The invention relates to the technical field of plant tissue culture, in particular to a sterile rapid propagation method of elaeagnus pungens stem tip meristem. The method comprises the following steps: immersing a stem section in a treating agent, and then washing with sterile water; inoculating the stem tip meristem into a primary induction culture medium which does not contain an exogenous antibacterial agent, and culturing to obtain sterile cluster buds; the method comprises the following steps: selecting robust rootless seedlings, and soaking the base parts of the seedlings in a solution containing IBA for pretreatment; then, the seedlings are inoculated into a rooting culture medium without exogenous auxin to be cultured, and complete regenerated plants are obtained; and hardening seedlings and transplanting. According to the invention, the long-acting silver ion / nano-silver composite protective layer is constructed, and the functional synergistic application of the protective layer in the whole process of subsequent primary culture, proliferation and seedling strengthening and rooting induction is realized; technical contradictions between efficient sterilization and explant activity maintenance, long-term sterile culture and endophyte pollution control, and high-quality root system induction and culture link disjunction in a traditional rapid propagation technology are synergistically solved.

The invention provides a bacillus velezensis strain and application thereof. The bacillus velezensis strain Amzn015 is firstly separated from the interior of a healthy bighead atractylodes rhizome plant, and the preservation number of the strain is CCTCC M 20252027. The bacterial strain has good antagonistic activity on Atractylodes macrocephala koidz root rot pathogen fusarium oxysporum, Fusarium oxysporum pathogen and Epicoccus catenarius, and the inhibition rates of the bacterial strain and the Fusarium oxysporum pathogen, the Fusarium oxysporum pathogen and the Epicoccus catenarius are respectively 85.75%, 76.43% and 72.11%. The bacterial strain has good antagonistic activity on Atractylodes macrocephala koidz root rot pathogen, the Fusarium oxysporum pathogen, the Fusarium oxysporum pathogen, the Fusarium oxysporum pathogen and the Epicoccus catenarius. The strain has a good treatment effect on bighead atractylodes rhizome root rot caused by fusarium oxysporum, the treatment effect is 66.2%, the strain is a potential biological agent, and the strain also has the capability of promoting plant growth. The bacillus velezensis disclosed by the invention has a wide development space and a good application prospect.

The invention discloses an environment-friendly potassium sulfate organic-inorganic compound fertilizer and a preparation method thereof, and relates to the technical field of agricultural fertilizers. The compound fertilizer disclosed by the invention adopts a core layer-wrapping layer double-layer structure and is matched with the plant endophyte-porous mineral microcapsules, so that the core problem of low survival rate of functional microorganisms in the prior art is solved. The porous mineral shell of the microcapsule provides multiple protection for microorganisms, and the fertilizer is prepared from natural and environment-compatible raw materials and has no potential environmental risk; the double-layer structure realizes quick-slow synergistic release of nutrients, integrates quick-acting supply of inorganic fertilizers, soil improvement of organic fertilizers and microbial synergistic functions, and can improve the soil structure, enhance the stress resistance of crops and improve the quality and yield of the crops. The process is simple and controllable, complex equipment is not needed, and large-scale popularization is easy.

The application discloses a broad-spectrum growth promoting bacterial agent combination for oil camellia and oil tea, a biological preparation and application thereof, and relates to the field of microbial technology.The endophytic bacteria are separated from healthy oil camellia plants, the abilities of the strains in dissolving phosphorus, fixing nitrogen, producing IAA, producing iron carrier and antagonizing the pathogenic bacteria of oil tea anthracnose are determined, the onion Burkholderia and Aspergillus niger with excellent comprehensive growth promoting functions are screened to prepare a combined bacterial agent, and the combined bacterial agent is verified to have a significant growth promoting effect on the oil camellia and the oil tea through a potting experiment.The combined bacterial agent promotes the growth, nutrient absorption and root development of the oil tea, significantly improves the enzyme activities related to the carbon, phosphorus and nitrogen cycles of the soil, and realizes the overall optimization of the "plant-soil" system.The application finds a broad-spectrum mixed bacterial agent suitable for the artificial planting of the oil camellia and the oil tea in a rocky desertification area, effectively improves the adaptability of the plants in the rocky desertification habitat, and provides a new microbial technical approach for the collaborative repair of multiple tree species in the area.

The present application belongs to the field of traditional Chinese medicine microbial technology, and particularly relates to a method for increasing the content of rapeseed pollen polysaccharide. The method is to increase the content of rapeseed pollen polysaccharide through microbial fermentation, and the microorganism is selected from plant endophytic bacteria and known microbial flora. The microorganism can be a single flora or a mixed flora. The microbial fermentation method can significantly increase the content of plant medicine polysaccharide, create a product probiotic environment, improve the pharmacological activity, and can be widely used in the deep fermentation production of plant medicines.

The invention relates to an agronomic composition comprising a Pseudomonas sivasensis or a Pseudomonas arvensis sp. nov., uses of this composition and use of a Pseudomonas sivasensis or a Pseudomonas arvensis sp. nov. as a protective endophyte.

Endophyte inoculant compositions, methods of making such compositions, methods of using such compositions, and physiologically altered plants treating with such compositions are disclosed. The endophyte inoculant composition may include one or more of endophyte strains WW5, WW6, WW7, and PTD1, which promote plant mineral nutrient acquisition and uptake, vigor, health, growth, and yield when applied to non-native host plants.

The invention provides a method for producing grain and fruit flavor ester vinegar through solid-state fermentation of famous wine Daqu system mash, and belongs to the technical field of vinegar brewing. The method comprises the following steps: fermenting famous wine Daqu strains, grains and fruits to prepare an alcohol-containing fermentation product; mixing the alcohol-containing leavening with a solid loose material and an acetic acid strain, carrying out acetic acid fermentation, aging fermented grains, carrying out oxygen-controlled fermentation, and carrying out soaking, smoking fermented grains, spraying vinegar and freezing treatment on the obtained vinegar fermented grains, so as to obtain the grain and fruit flavored ester vinegar. According to the method, famous wine yeast flora and endophytic flora in fruits are synergistically domesticated and controlled in the stages of mash or fermented grain fermentation, acetic fermentation and aged fermented grain oxygen-controlled fermentation, so that the aromatic ester vinegar with both grain brewing flavor and fruit fragrance is obtained, and the aromatic ester vinegar is unique in flavor and mellow in taste.

The invention provides an application of an enterobacter and bacillus mixture in treatment of strawberry anthracnose and void disease, by exploring reasons of strawberry void disease, endophyte enterobacter of the enterobacter can generate voids in strawberry stem bases to inhibit germination of anthrax spores, antibacterial effects of different enterobacter are compared, and the enterobacter and bacillus mixture can be applied to treatment of strawberry anthracnose and void disease. A method capable of more effectively inhibiting strawberry anthracnose and reducing cavities is researched, meanwhile, the situation that the cavities can be reduced and germination of colletotrichum gloeosporioides spores can be further inhibited while bacillus and enterobacter are combined for use is found, and the disease resistance of strawberries can be effectively improved. The enterobacter and bacillus mixture provided by the invention has good biocompatibility, is simple to use, is not easy to generate resistance, and has broad prospects in the fields of microorganisms and biological control.

The application discloses a kind of planting methods for improving the content of Dendrobium candidum polysaccharide, covers seedling pretreatment, substrate preparation, light temperature water stress regulation, microbial reinforcement, selenium-silicon nutrition supplement and intelligent harvesting decision.By red light / blue light combination (3:1) and pulse drought activation secondary metabolism, inoculate beta-fructan endophyte Paenibacillus polymyxa and add chelated selenium-silicon foliar fertilizer, combined with low temperature and low humidity adversity in the later growth stage, the stem polysaccharide content reaches 38%-42%.System integration LED light source, EC sensing irrigation and bacterial agent spraying equipment, realize standardization production.Embodiment shows that the method is 35-40% higher than traditional planting polysaccharide, and there is no gene editing risk, in line with GAP specification.

The invention discloses a grape wine color protection method based on alternaria MG1, and belongs to the technical field of fermented wine brewing. According to the method, alternaria MG1 is taken as a research object, a phenylpropane metabolic pathway of alternaria MG1 is activated through various stimulation methods such as ultraviolet radiation, exogenous addition of oligomeric proanthocyanidins, co-culture with other endophytic filamentous fungi and the like, and the alternaria MG1 and saccharomyces cerevisiae are subjected to mixed fermentation, so that an optimal color protection process for grape wine color is explored. The invention deeply discusses the influence of the mixed fermentation on the color and the related quality characteristics of the wine, provides a new theoretical support and a process optimization strategy for the application of the multi-strain synergistic fermentation based on the grape endophyte in wine brewing, and has important guiding significance for production practice.

This invention discloses a strain of Codonopsis pilosula endophytic fungus that produces extracellular polysaccharides and its applications. This Codonopsis pilosula endophytic fungus is a short-spore bacterium, named... Brevibacillus schisleri CpRG-1. The Codonopsis pilosula endophytic bacteria of the present invention exhibit excellent ability to produce extracellular polysaccharides, producing high concentrations of extracellular polysaccharides, which can greatly reduce the cost of extracting polysaccharides from the medicinal herb Codonopsis pilosula; at the same time, the extracellular polysaccharides produced by the Codonopsis pilosula endophytic bacteria not only have antibacterial and hypoglycemic activities, but also can promote the healing of diabetic foot ulcers and improve the intestinal microbiota, providing new ideas for the development of antibacterial drugs, diabetes prevention foods and drugs, and diabetic foot ulcer treatment foods and drugs.

The invention belongs to the technical field of ligusticum wallichii root rot resistance, and particularly discloses a ligusticum wallichii endophytic composite flora and application thereof. According to the present invention, the composite bacterial flora comprises Bacillus subtilis, Fusarium acuminatum (Fusarium acuminatum) and Purpureocillium lilacinum (Purpureocillium lilacinum), and the composite bacterial flora comprises Bacillus subtilis, Fusarium acuminatum (Fusarium acuminatum) and Purpureocillium lilacinum (Purpureocillium lilacinum (Purpureocillium lilacinum (Purpureocillium lilacinum), the composite flora can be stably colonized in ligusticum wallichii tissue culture seedlings and continuously exerts the antagonistic effect on root rot, so that public nuisance chemical prevention and treatment agents are replaced. The method has the advantages that endophytes of ligusticum wallichii are reasonably combined, so that the colonization instability and insecurity caused by direct field application of a conventional exogenous biocontrol microbial inoculum are avoided; and meanwhile, a simplified ligusticum wallichii micro-ecosystem is constructed, and the function and the structure of the ligusticum wallichii micro-ecosystem are more stable by adopting a cross-boundary combination of multiple strains of bacteria and fungi.

The present invention relates to molluscicidal compositions. In particular, the invention relates to molluscicidal compositions comprising loline species (e.g., loline alkaloids) and endophyte- infected plant biomass and methods and uses thereof.

Described herein is a modified endophyte, methods of modification and uses thereof. The modified endophyte may be modified to enhance traits / characteristics for properties such as fungicide resistance / tolerance. Modification may be via isolation, in vitro culturing, selection and reintroduction to a plant host. The modified endophytes and methods may be used to produce endophytes and related plant:endophyte symbiotic relationships with enhanced properties such as enhanced fungicide resistance.

The invention relates to bacillus amyloliquefaciens with antibacterial, enzyme-producing and growth-promoting functions and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms. The strain is a plant endophyte separated and screened from the root of a genuine medicinal material Chinese mugwort in Hubei province, and is preserved in the China Center for Type Culture Collection on March 20, 2025, and the preservation number is CCTCC NO: M 2025535. The strain has the advantages of short fermentation time, strong antibacterial ability, simple culture conditions and the like, and has both enzyme production activity and growth promoting effect. Due to the multifunctional characteristics, the bacillus subtilis has huge application potential in the fields of medical treatment and public health, food preservation, agricultural disease prevention and control, industrial enzyme preparation production and the like.

This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and / or seedlings (e.g., cereal seeds and / or seedlings) with heterologous seed-derived bacterial endophytes.