298 results about "Endophyte" patented technology

The invention provides a penicilliums p. strain and application thereof. Eucalyptus endophyte is a penicilliums p. strain 1, and the collection number is CGMCC No.4297. The eucalyptus endophyte inoculated to eucalyptuses can improve the low phosphorus stress resistance of the eucalyptuses and promote phosphorus absorption of the eucalyptuses. Because the eucalyptus endophyte is used for promoting the phosphorus absorption of the eucalyptuses, and when the strains are inoculated to the eucalyptus plants, the activities of the plant phosphatase and the soil phosphatase can be improved, the pH value of the soil rhizosphere can be reduced, and the plant P utilization rate is greatly improved; therefore, the purpose of promoting normal growth of the plants in the low phosphorus soil is fulfilled, and the utilization rate of the soil phosphate fertilizer is greatly reduced.

The invention discloses a burkholderia pyrrocinia JK-SH007 which is classified and named as burkholderia pyrrocinia and preserved in China center for type culture collection (CCTCC) with the preservation number of M 209028 and the preservation date of February 18th, 2009. The invention also discloses the application of the burkholderia pyrrocinia in controlling dothiorella gregaria and promoting poplar to grow. The invention further provides a bactericide for controlling dothiorella gregaria, which is obtained by inoculating the JK-SH007 in a culture medium to be fermented and cultured. The JK-SH007 is plant endophyte which has strong inhibiting effect for botryosphaeria dothidea of the poplar and antagonism for various pathogenic fungis, and has the advantages of simple culture condition, easy storage and commercial process as well as good developing and applying prospect.

The invention discloses an extraction and purification method of total plant endophyte genome DNA for colony analysis. The method comprises the following steps of: shearing and grinding the surface bacteria removed fresh plant tissues into paste, soaking the plant tissues into sterilized phosphate buffer solution, performing thermostatic shaking for 1 hour in a table concentrator to separate out microbial cells from the plant tissues, standing, transferring the suspension to a centrifuge tube, performing centrifugal collection on microbial bacteria, then adding lysing solution, shaking and uniformly mixing the solution to release DNA from the cells, repeatedly extracting protein by chloroform-isoamylol, performing centrifugal sedimentation with isopropanol and washing with 70 percent ice ethanol, performing purification by using a DNA specific centrifugal adsorption column to obtain total crude extracted genome DNA, and finally, performing polymerase chain reaction (PCR) amplificationby using special primers to obtain high-quality plant endophyte specific DNA fragments for subsequent analysis. The invention has the advantages that: the method is simple and effective, high in quality, low in pollution and the like, and provides high-quality guarantee for comprehensively and completely researching a plant endophyte colony structure.

The invention discloses a bacillus pumilus JK-SX001 which is classified and named bacillus pumilus and collected in a China center for type culture collection (CCTCC) and has the collection number ofM 209026 on February 18th, 2009. The invention also discloses applications of the bacillus pumilus in preventing and treating dothiorella gregaria and promoting the growth of a poplar. The invention also provides a bactericide for preventing and treating the dothiorella gregaria, which is obtained by vaccinating the JK-SX001 in a culture medium to ferment and culture. The JK-SX001 is a plant endophyte, has obvious inhibiting effect on dothiorella gregaria, simple culture condition, easy storage and favorable development and application prospect and is easy for industrialized production.

The invention provides bacillus subtilis BS193 for preventing and controlling a potato late blight and application of bacillus subtilis BS193 and belongs to the technical field of crop disease prevention and control. A strain is Bacillus subtilis BS193 and has been recorded and preserved by China General Microbiological Culture Collection Center at March 27, 2017 with a preservation number being CGMCC No. 13936. The strain inhibits growth of germs of the potato late blight by generating active substances and expresses better preventing and controlling effect to the potato late blight. The fungicide can be used for atomizing or root irrigation of a potato plant when being applied and can also be used by being mixed with an organic fertilizer to prepare a bacterial fertilizer. The bacillus subtilis BS193 provided by the invention is obtained by being extracted from a tomato plant body, belongs to endophyte, is nontoxic, does not have pathogenicity, and thus having a good application prospect in biological prevention and control of the potato late blight.

The invention relates to Pennisetum purpureum Schum-derived bacillus megaterium and a use thereof and belongs to the technical field of microbes. The Pennisetum purpureum Schum-derived bacillus megaterium pp02 has a class name of Bacillus megaterium pp02, is preserved in the China general microbiological culture collection center (CGMCC) and has a preservation number of CGMCC 9415. The Pennisetum purpureum Schum-derived bacillus megaterium has iron-producing carrier, azotification and IAA production characteristics, has an IAA yield of 10.57 microgrammes per milliliter, has a strong plant root colonization ability and good salt resistance, can grow in a culture medium containing 5% of NaCl (50g/L) and is an excellent plant growth promoting endophyte strain. Through infection of hybrid pennisetum with the Pennisetum purpureum Schum-derived bacillus megaterium, plant salt resistance is substantially improved, plant growth is promoted, and fresh weight, dry weight and height of the plant above the ground are substantially improved. The Pennisetum purpureum Schum-derived bacillus megaterium has important development and application prospects in study of hybrid pennisetum microbial fertilizer and promotion of growth and development of plants in saline marginal land.

The invention provides garlic endophyte for accelerating a black garlic fermentation process and an application thereof in the black garlic fermentation, relates to the garlic endophyte and the application thereof in the black garlic fermentation, and belongs to the field of black garlic fermentation from garlic endophyte. The garlic endophyte for accelerating the black garlic fermentation process is Bacillus subtilis S<8nyzx-1> which is preserved in the common microorganism center of China general microbiological culture collection center (CGMCC) with a preservation number of CGMCC No. 6647.

The invention discloses a separation method for plant endophyte. The separation method comprises the steps of: sampling, surface sterilization, tissue grinding, separation and pure cultivation, and particularly comprises sampling health plants, storing in classification, soaking in an ethanol solution, soaking again in an sodium hypochlorite solution, washing by sterile water, weighing 0.3-0.3 g, adding the sterile water and grinding. The separation method further comprises the steps of: diluting the ground slurry in gradients, plate cultivating after coating, repeating each processing for 3-5 times, selecting single colonies according to eight phenotypic characteristics of bacterial colonies, consisting of shape, size, humidity, color, texture, surface, edge and transparency, for pure cultivation, and moving the pure cultivated single colonies into the test tube slants for preservation. Through the application of the separation method for plant endophyte, the diversity separation result of the plant endophyte is effectively prevented from being manually enlarged or lost; and the separation method for plant endophyte plays a very important role in researching the composition characteristics of plant endophyte colonies and enriching the gene resources of the endophyte.

Materials and methods for improving plant traits and for providing plant fitness benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.

The invention discloses a plant endophyte with a category name of Enterobacter sp. and a complete name of Enterobacter sp. SaCS20. The strain is preserved at China General Microbiological Culture Collection Center (CGMCC) on March 28th, 2013, and has a preservation number of CGMCC NO: 7381. The invention also discloses the application of the plant endophyte in promoting paddy rice growth, improving paddy rice yield, or promoting trace element zinc accumulation in paddy rice. The Enterobacter sp. CGMCC 7381 provided by the invention is separated from the root system of a zinc hyperaccumulator sedum alfredii. The plant endophyte has no pathogenicity upon paddy rice, and can be successfully colonized into paddy rice root systems. With the plant endophyte, paddy rice growth can be promoted, rhizosphere soil available zinc content can be increased, and grain zinc enrichment can be promoted. The application provided by the invention is simple to operate, and is safe and economical. With the plant endophyte and the application, soil micronutrient fertilizer investment can be reduced, and crop yield can be increased.

The invention provides an endophyte for promoting the growth of plants and the remediation of heavy metal-contaminated soil and application thereof. Bacterial liquid of endophyte SLS18 is utilized to dip-dye Sorghum bicolor L. in a mode of seed bacteria dipping to colonize the endophyte SLS18 into the Sorghum bicolor L. to accompany each stage of the growth of the Sorghum bicolor L., so that the growth of the Sorghum bicolor L. on heavy metal-contaminated marginal land and the remediation of soil are promoted. The endophyte SLS18 is separated from the stem of a hyperaccumulator, namely pokeberry root, is obtained through screening, and is an endophyte which can be successfully colonized in the Sorghum bicolor L., has a remarkable effect of promoting the growth of the Sorghum bicolor L. and has the heavy metal resistance. A method is easy to operate, and is safe and economic, no new pollution is introduced into soil environment, heavy metal-contaminated marginal land resources can be effectively utilized so as to avoid the rival of energy crops and food crops against finite cultivated land, and heavy metal-contaminated land can also be effectively remediated.

The invention relates to a Salvia miltiorrhiza endophyte and its use in the improvement of danshinolic acid B accumulation in the Salvia miltiorrhiza hairy root culturing process. The Salvia miltiorrhiza endophyte is Pseudomonas psychrotolerans LG4 concretely, and has a preservation number of CCTCC NO:M2015085. The Salvia miltiorrhiza endophytec an promote the accumulation of a phenolic acid substance (danshinolic acid B) in Salvia miltiorrhiza hairy root.

The invention discloses a method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating the pistacia chinensis bunge seeds. The method mainly comprises the following steps of: sterilizing the pistacia chinensis bunge seeds, of which the shells are removed by using ethanol and sodium hypochlorite; soaking the seeds in water to perform ultrasonic processing; and flushing the seeds, and then inoculating the seeds to a seed germination culture medium to obtain aseptic robust seedlings. By adoption of the method, the difficulty that the germination rate of the seeds is influenced by pollution caused by inexhaustive sterilization and long sterilization time is solved, and more importantly, the germination rate of the seeds is increased, the seedlings grow robustly, and a basis is provided for tissue culture and genetic transformation of pistacia chinensis bunge.

The invention provides a modification method for improving bacteria heavy metal adsorption capacity, an adsorbent and the application of the method. The method takes glutaraldehyde as a cross-linking agent, and comprises the step of: connecting poly allyamine hydrochloride (PAA/ HCl) to the surface of plant endophyte Pseudom onas sp. LK9 thallus so as to cause the surface of the thallus to have a great deal of amino groups and further improve the adsorption effect of the thallus for heavy metals. The maximum adsorbing capacities of the obtained adsorbent for the heavy metals Pb<2+>, Cd<2+> and Cu<2+> are respectively 269.9mg/ g, 145.0mg/ g and 148.0mg/ g; and compared with an LK9 adsorbent which is not modified, the adsorbing capacities are respectively increased by 192.98mg/ g, 121.85mg/ g and 128.35mg/ g. The invention has the characteristics of being simple in method, simple and convenient to operate, remarkable and rapid in adsorption effect, environment-friendly and the like, thus having great application potential in the aspect of heavy metal pollution water treatment.

The invention obtains an endophyte of a dioscorea zingiberensis by steps of field sampling, sample surface sterilization, isolation of microorganism, liquid culture and the like; the strain is identified as bacillus licheniformis, which can produce diosgenin of the dioscorea zingiberensis and can be used as production strain for fermentation.

The invention discloses a strain of antagonistic bacteria for preventing and controlling poplar colletotrichumgloeosporioides and application thereof. The antagonistic bacteria are bacillus atrophaeus XW2, and the microbial collection number is CGMCC NO. 7689. The bacillus atrophaeus XW2 is obtained through plate face-to-face culturing and screening of poplar endophytes via a flat plate, has an obvious antagonistic effect on the poplar colletotrichumgloeosporioides, can be used for effectively inhibiting the growth of poplar colletotrichumgloeosporioides hypha and germination of spores and preventing and controlling plant diseases caused by the poplar colletotrichumgloeosporioides, is a biological prevention and control potential strain with high protection effect and good environmental safety and has good development and application prospect.

The invention provides a novel flavonoid glycoside and isoquinoline alkaloid complex structure, preparation, antibacterial application and three complex carrier-free nano-drugs with self-assembly performance. The structural general formula of the complex is shown in the specification, the complex has excellent selective antibacterial ability, and the activity of endophyte escherichia coli and probiotics bacillus subtilis and enterococcus faecium is not influenced while pathogenic bacteria staphylococcus aureus is killed; meanwhile, the compound has a good inhibition effect on multiple drug-resistant staphylococcus aureus, and is superior to norfloxacin, oxacillin, tetracycline and ciprofloxacin. The nano-drug formed by self-assembling the three complexes is uniform in form and good in dispersity and stability, gel or fiber is formed under the condition that auxiliary materials are not needed, and support is provided for development of a carrier-free pure drug delivery preparation.

The invention discloses a method for screening endophytic fungi capable of improving the ability of crops to resist fungal diseases. The steps include: 1) collecting plant materials to obtain plant-free transplanted seedlings without any disease; 2) separating endophytic fungi from plants fungi; 3) Prepare endophyte spore suspensions from the isolated endophytic fungi, and mycelial suspensions from endophytic fungi that do not produce spores; 4) Inoculate endophytic fungal spores or mycelial suspensions until the 5) Infect the transplanted seedlings inoculated with endophytic fungi and the transplanted seedlings not inoculated with endophytic fungi with pathogenic fungi, and observe the control after one month Effect, the endophytic fungi with a control effect greater than 80% are endophytic fungi that can improve the ability of the crops to resist fungal diseases.

The invention relates to a novel method for fermenting endophyte by taking sweet potato leaves as a basic material and belongs to the field of microbial fermentation. As a depside, chlorogenic acid is produced through a shikimic acid pathway generally and exists in plants such as Cortex Eucommiae, Flos Lonicerae and Flos Chrysanthemi Indici. As an important bioactivator and valuable traditional Chinese medicine, the chlorogenic acid has the antibacterial, antiviral and anti-tumor effects and the like. At present, since the chlorogenic acid is mainly extracted from natural plants but extraction technologies are complex and yield is low, seeking a novel method for producing the chlorogenic acid is particularly important. The novel method has the advantages that the endophyte capable of producing the chlorogenic acid can be obtained by fermentation of the simple and available sweet potato leaves serving as a main material, so that the chlorogenic acid synthesis capability of the endophyte is enhanced substantially, total output of the chlorogenic acid in fermentation liquor can reach 54.60mg l<-1> which is 23.90 times of initial fermentation output (1.58mg l<-1>) of RP5, output of total chlorogenic acid includes the chlorogenic acid and two kinds of isomerides thereof, and output of the two kinds of isomerides can reach 128.04mg l<-1> which is 31.74 times of initial fermentation output (1.74mg l<-1>). The novel method has considerable economic benefit.

The invention discloses a rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin and the preservation number of the Pantoea sp. Sd-1 is CGMCC No. 6698. The Pantoea sp. Sd-1 is a bacterial strain which can grow on a culture medium with alkali lignin as a unique carbon source. After the Pantoea sp. Sd-1 is cultured for four days in a liquid culture medium containing 3g/L alkali lignin and supplemented with 1% of glucose and 0.5% of peptone, the fading rate and the degradation of lignin respectively reach 52.38% and 69.06%. And in a liquid culture medium containing 10g/L rice straws and supplemented with 1% of glucose and 0.5% of peptone, the degradation of straws can reach 54.51 at most.