163 results about "Endophyte" patented technology

The invention provides a rice endophyte for promoting rice growth and reducing cadmium as well as a preparation method and application of the rice endophyte, and relates to the field of environmental microbiology and agricultural microorganisms. The rice endophyte is stenotrophomonas, is named as Stentrophomonas sp.R5, is preserved in the China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the postcode is 430072, the preservation number is CCTCC NO: M 20231758, and the preservation date is September 20, 2023. The rice endophyte not only has the capabilities of producing IAA, dissolving phosphorus, producing siderophores and the like for promoting the growth and development of rice, but also has an efficient inhibition effect on absorption and transportation of cadmium in the rice, particularly, the cadmium reduction amplitude of mature rice grains reaches up to 57.69%, and the rice endophyte has little influence on microbial communities in a soil environment, and has a good application prospect. Excessive interference is not caused, and conversion of available nutrients in the soil is promoted.

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention discloses endophyte Schizophyllanthus commune CY01-1H for improving synthesis of jasmonic acid and dendrobine of dendrobium nobile seedlings and application of the endophyte Schizophyllanthus commune CY01-1H, and belongs to the technical field of plant secondary metabolism regulation and control and microorganisms. The preservation number of the endophyte CY01-1H is CGMCC (China General Microbiological Culture Collection Center) No.42003; the strain is preserved in China General Microbiological Culture Collection Center on May 12, 2025, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the endophytic fungus CY01-1H obtained through separation comes from a healthy dendrobium nobile plant, the content of jasmonic acid of dendrobium nobile and accumulation of dendrobine can be increased by planting the endophytic fungus CY01-1H in dendrobium nobile, the normal growth state of the plant is not affected, and wide application prospects are brought for improving the quality of the dendrobium nobile medicinal material.

This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and / or seedlings (e.g., cereal seeds and / or seedlings) with heterologous seed-derived bacterial endophytes.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

Disclosed are endophyte inoculant compositions, methods for making such compositions, methods for using such compositions, and physiologically altered plants treated with such compositions. The endophyte inoculant compositions may include one or more of the endophytic species WW5, WW6, WW7, and PTD1, and when applied to a non-native host plant, promote plant mineral nutrient acquisition and uptake, vigor, health, growth, and yield.

The present invention discloses a strain of Nocardia YINM00009 and its application in the preparation of cyclic lipopeptide compounds, belonging to the field of microbial technology. The Nocardia YINM00009 is an endophyte of Polygonatum daturae in Kunming, Yunnan Province, and can metabolize a large number of novel cyclic lipopeptide compounds after fermentation; the fermentation step mainly includes: fermenting the strain under suitable culture conditions to obtain a fermentation broth, after solvent extraction and concentration, combined with column chromatography, 9 cyclic lipopeptide compounds can be prepared, the total concentration of the 9 cyclic lipopeptide compounds in the fermentation broth can reach 10.2~16.5mg / L, and the purity after extraction and separation can reach more than 99%, of which 2 cyclic lipopeptide compounds have strong anti-tumor cytotoxic activity. The method is low in cost, simple in process, and environmentally friendly, and can be used for the mass production and preparation of such cyclic lipopeptide compounds, which not only meets the needs of a low-carbon economy, but also lays the foundation for the production of cyclic lipopeptide compounds and the development of related products in the later stage.

The invention relates to the technical field of agricultural planting, in particular to a method for promoting rice growth by using metarhizium anisopliae as rice endophyte, which comprises the following steps: step 1, preparing metarhizium anisopliae spore suspension with the concentration of 1 * 10 * 8 / mL; step 2, soaking rice seeds in the spore suspension for 12 hours; 3, the treated seeds are subjected to soil culture or water culture, and the culture conditions are that the day and night temperature is set to be 26 DEG C, and the photoperiod is set to be 16 L: 8 D; and 4, colonizing the metarhizium anisopliae in root, stem and leaf tissues of the rice to promote the growth of the rice and enhance the resistance to brown planthopper and spodoptera frugiperda. According to the invention, various growth indexes of rice can be obviously improved, and the highest synergism of plant height, root length, fresh weight and dry weight under the soil culture condition is 37.9%, 10.2%, 22.7% and 19.9% respectively; under the water culture condition, the contents are respectively 46.0%, 14.8%, 50.6% and 13.0%.

The present invention relates to the delivery of a methane inhibitor to a plant or a grazing animal. This is achieved by infecting grass with an endophyte which has been modified to produce the methane inhibitor in planta. The endophyte produces the methane inhibitor in planta, which may be consumed by the grazing animal.

The present invention discloses a seed endophyte for preventing and treating wheat fungal diseases and its application, belonging to the technical field of crop planting and crop disease biological control. The wheat endophyte (Pseudescherichia vulneris) GSE25 in the present invention was deposited in the China General Microbiological Culture Collection Center (CGMCC) on November 21, 2022, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC NO.26158. The GSE25 strain has the ability to significantly reduce the pathogenicity of gibberellins and stripe rust on wheat plants, and can significantly reduce the mycotoxins produced by pathogens on wheat grains, has a promoting effect on crop growth and development, and has a broad-spectrum effect. The GSE25 strain of the present invention is of great value in the healthy growth, yield increase, control of pathogenic fungi and mycotoxin pollution of crops.

The invention belongs to the technical field of microorganisms, and particularly relates to a kelp endophyte, i.e., presteria megaterium H403 and an application of the kelp endophyte, i.e., presteria megaterium H403. In order to solve the problems that the kelp seedling raising period is long and the cost is high, an endophytic bacterium is separated from kelp and is named as presteria megaterium H403, a co-culture experiment shows that the strain can remarkably improve the germination rate and the growth rate of kelp seedlings, and the length of kelp in a treatment group added with 14 * 10 < 6 > cfu bacterial liquid is 3.6 times that of a control group. The invention discloses a molecular mechanism for promoting the growth of the kelp through multiple ways by virtue of the Pristeria megaterium H403, a theoretical basis is provided for developing a microorganism-assisted seedling raising technology, the kelp culture cost is expected to be reduced, and the sustainable development of the industry is promoted.

The application discloses application of a bacteria-sugar composition in stress resistance regulation of saline-alkali soil and belongs to the technical field of plant growth regulation. The microorganism is Bacillus, and the plant source extract is a salvia miltiorrhiza-safflower composite polysaccharide. The biological preservation number of the Bacillus in the composition is CGMCC No. 25566, the bacterial concentration is 8.5*10 5 CFU / mL, and the salvia miltiorrhiza-safflower composite polysaccharide is prepared by mixing salvia miltiorrhiza and safflower as raw materials according to a mass ratio of (10~1):(1~10) and adopting polysaccharide extraction, and the concentration is 1 ug / mL. The Bacillus with the biological preservation number of CGMCC No. 25566, which is screened from medicinal plant salvia miltiorrhiza symbiotic microorganism, is combined with the salvia miltiorrhiza-safflower composite polysaccharide to obtain a bacteria-sugar composition for regulating crop tolerance to moderate to severe saline-alkali soil, and the application of salvia miltiorrhiza endophyte and plant source polysaccharide is further developed.

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of Erwinia gerundensis which provides bioprotection and / or biofertilizer phenotypes to plants into which it is inoculated. The present invention also discloses plants infected with the endophyte and related methods.

The invention discloses an endophyte for inhibiting corn spider mites. The invention provides bacillus velezensis which is bacillus velezensis 2025-BC248L1-1, and the preservation number of the bacillus velezensis 2025-BC248L1-1 is CGMCC (China General Microbiological Culture Collection Center) No. 33929. The invention also provides any one of the following applications of the bacillus velezensis or the culture thereof: A1) preventing plant diseases and insect pests; a2) treating plant diseases and insect pests; and A3) inhibiting phytopathogen. The endophyte with the activity of antagonizing the corn pathogenic bacteria is separated from corn leaves, the classification status of the endophyte is accurately determined by a molecular identification method, and the endophyte is identified as the bacillus. The endophyte is subjected to a biocontrol experiment of field endophyte liquid on spider mites, a new microbial resource is provided for biological control of corn diseases, and the endophyte is expected to be developed into a biological control preparation, so that the use of chemical pesticides can be reduced, and the environmental pollution is reduced.

The present invention relates to a composition comprising:an isolated endophyte, wherein the endophyte is a strain of Priestia aryabhattai, andan adjuvantwherein the Priestia aryabhattai strain has Accession Number DSM 34352.

The application discloses a new antibacterial compound and a preparation method and application thereof, and the new antibacterial compound is obtained by fermenting endophytic fungi (Epicoccum latusicollum) under stress of pathogenic fungi (Fusarium oxysporum), filtering, discarding mycelia, enriching the fermentation liquor by using a macroporous adsorption resin, and adopting high performance liquid preparation separation, and the chemical name of the new compound is (6E,8E)-13-acetoxy-3-hydroxy-8,10,12,14-tetramethylhexadeca-6,8-dienoic acid. The new compound obtained by fermenting endophytic fungi under stress of pathogenic fungi has significant antibacterial activity, and the application provides a scientific basis for mining endophytic fungi antibacterial natural compounds based on stress effects.

The invention relates to the technical field of microorganisms, in particular to a desert plant endophyte, a microbial agent and application, the desert plant endophyte is Pristeria aryabhattai HB-4 and is preserved in the China Center for Type Culture Collection on November 17, 2025, and the preservation number of the desert plant endophyte is CGMCC No.36665. The invention further relates to a method for preparing the desert plant endophyte. The strain can efficiently produce indoleacetic acid and has remarkable growth promoting and stress resistance functions. After the strain is inoculated, the fresh weight, the dry weight, the stem length, the chlorophyll content and the catalase activity of wheat are remarkably improved, and the malondialdehyde content is reduced. The invention further provides a microbial agent containing the strain and application of the microbial agent in the aspects of promoting plant growth and improving stress resistance, the microbial agent is particularly suitable for cultivation of crops such as wheat in drought and barren environments, and a new strain resource is provided for development of agricultural microbial preparations.

The present invention relates to the field of biotechnology, and in particular to a lotus root endophyte Bacillus Velez subtilis strain LS8 and its application. The present invention provides a lotus root endophyte Bacillus Velez subtilis LS8, which is deposited in the General Microbiology Center of the China Microorganism Culture Collection Committee with a deposit number of CGMCC No. 24563. The lotus root endophyte Bacillus Velez subtilis LS8 and its biocontrol agent have the characteristics of a wide range of antifungal effects and significant antagonistic effects, and can be used to prevent and control plant fungal diseases, thus having good industrial application prospects. At the same time, the lotus root endophyte Bacillus Velez subtilis strain LS8 provided by the present invention has a significant preventive effect in potted plant experiments and has great practical value for the prevention and control of crop diseases.

The invention relates to the technical field of microorganisms, in particular to two strains of Sporosarcina koreensis and application thereof, the Sporosarcina koreensis is Sporosarcina koreensis HPN-QB-3-R '2.2, the preservation number of the Sporosarcina koreensis HPN-QB-3-R' 2.2 is CGMCC No: 34277, and the preservation number of the Sporosarcina koreensis HPN-QB-3-R '2.2 is CGMCC No: 34277. The invention discloses Sporosarcina koreensis HPN-QB-3-R '2.3, and the preservation number of the Sporosarcina koreensis HPN-QB-3-R' 2.3 is CGMCC (China General Microbiological Culture Collection Center) No: 34278. The Korean sporosarcina HPN-QB-3-R '2.2 and the Korean sporosarcina HPN-QB-3-R' 2.3 are separated and screened from roots of a medicinal plant pseudo-ginseng for the first time, the HPN-QB-3-R '2.2 has good effects of resisting oxidation, repairing and the like, the HPN-QB-3-R' 2.3 has good effects of resisting oxidation, moisturizing and the like, the application field and application prospect of the pseudo-ginseng endophyte are widened, and a foundation is laid for development of biological fermentation skin care products.

The application discloses a plough head mold GXU-F34 for promoting linaloe, and the plough head mold GXU-F34 is obtained by purifying and separating samples cut from old trees and can be used for promoting linaloe. The plough head mold GXU-F34 is a plant endophyte, can continuously produce some enzymes to stimulate the defense system of Aquilaria plants, and a series of enzymes produced by saprophytic fungi microorganisms play an important role in the defense mechanism of Aquilaria plants, and finally lead to the production of linaloe.

The invention discloses an amorpha fruticosa aerial seeding seed pelleting treatment method and equipment for increasing the germination rate, and particularly relates to the field of forest tree seed treatment.The amorpha fruticosa aerial seeding seed pelleting treatment method.The amorpha fruticosa aerial seeding seed pelleting treatment method comprises the steps that S1, amorpha fruticosa seeds are cleaned, disinfected and soaked, washed with clear water after soaking is completed, and then naturally aired; s2, collecting a fresh koelreuteria paniculata seed sample to prepare an endophyte solution, preparing an adhesive, and uniformly stirring the adhesive to fully dissolve; s3, adding the amorpha fruticosa seeds and an adhesive into a stirrer, uniformly stirring, then adding the endophyte liquid, continuously stirring, then selecting medical stone and bentonite as carriers, and quickly and uniformly wrapping the seeds to obtain a mixture; and step S4, mixing the mixture, and then carrying out granulation treatment. Through the synergistic effect of multi-step pretreatment and the pelleting process, the germination rate and the survival rate of the amorpha fruticosa seeds are remarkably improved, the physical characteristics of the seeds are optimized through pelleting treatment, and the aerial seeding adaptability is greatly improved.

This disclosure relates to compositions and methods of producing compositions for improving plant traits. This disclosure provides methods of identifying native endophytes, genetically engineering said endophytes, and recolonizing plant(s) which the endophytes were isolated from. Further, this disclosure provides compositions made by the methods disclosed herein and using the same.

The invention relates to the technical field of green prevention and control of agricultural diseases and insect pests, and discloses a green prevention and control method for agricultural planting diseases and insect pests, a composition takes a core preform carrying negative charges as a core and takes functional chitosan carrying positive charges as a shell, and a core-shell structure is formed through electrostatic self-assembly. The core preform comprises an endophyte active matter, an anionic inner shell skeleton polymer and chelated medium and trace nutrient elements; the functional chitosan is prepared by grafting signal oligosaccharide and an environmental response group on a chitosan molecular chain. According to the composition disclosed by the invention, an endophyte active matter is effectively isolated through a core-shell structure, the storage stability of the composition is improved, and slow release is realized; meanwhile, the direct biocontrol effect of the active matter, the plant immunity induction effect of the functional chitosan and the nutritional supplement effect of the medium and trace nutrient elements are organically combined, multi-mechanism synergistic interaction is achieved, and the field control effect is excellent.

Endophyte combinations, compositions, and methods of using the endophyte combinations and compositions for improving plant and algae productivity and tolerance to environmental stresses.

The invention relates to the technical field of plant tissue culture, in particular to a sterile rapid propagation method of elaeagnus pungens stem tip meristem. The method comprises the following steps: immersing a stem section in a treating agent, and then washing with sterile water; inoculating the stem tip meristem into a primary induction culture medium which does not contain an exogenous antibacterial agent, and culturing to obtain sterile cluster buds; the method comprises the following steps: selecting robust rootless seedlings, and soaking the base parts of the seedlings in a solution containing IBA for pretreatment; then, the seedlings are inoculated into a rooting culture medium without exogenous auxin to be cultured, and complete regenerated plants are obtained; and hardening seedlings and transplanting. According to the invention, the long-acting silver ion / nano-silver composite protective layer is constructed, and the functional synergistic application of the protective layer in the whole process of subsequent primary culture, proliferation and seedling strengthening and rooting induction is realized; technical contradictions between efficient sterilization and explant activity maintenance, long-term sterile culture and endophyte pollution control, and high-quality root system induction and culture link disjunction in a traditional rapid propagation technology are synergistically solved.

The invention provides a bacillus velezensis strain and application thereof. The bacillus velezensis strain Amzn015 is firstly separated from the interior of a healthy bighead atractylodes rhizome plant, and the preservation number of the strain is CCTCC M 20252027. The bacterial strain has good antagonistic activity on Atractylodes macrocephala koidz root rot pathogen fusarium oxysporum, Fusarium oxysporum pathogen and Epicoccus catenarius, and the inhibition rates of the bacterial strain and the Fusarium oxysporum pathogen, the Fusarium oxysporum pathogen and the Epicoccus catenarius are respectively 85.75%, 76.43% and 72.11%. The bacterial strain has good antagonistic activity on Atractylodes macrocephala koidz root rot pathogen, the Fusarium oxysporum pathogen, the Fusarium oxysporum pathogen, the Fusarium oxysporum pathogen and the Epicoccus catenarius. The strain has a good treatment effect on bighead atractylodes rhizome root rot caused by fusarium oxysporum, the treatment effect is 66.2%, the strain is a potential biological agent, and the strain also has the capability of promoting plant growth. The bacillus velezensis disclosed by the invention has a wide development space and a good application prospect.

The invention discloses an environment-friendly potassium sulfate organic-inorganic compound fertilizer and a preparation method thereof, and relates to the technical field of agricultural fertilizers. The compound fertilizer disclosed by the invention adopts a core layer-wrapping layer double-layer structure and is matched with the plant endophyte-porous mineral microcapsules, so that the core problem of low survival rate of functional microorganisms in the prior art is solved. The porous mineral shell of the microcapsule provides multiple protection for microorganisms, and the fertilizer is prepared from natural and environment-compatible raw materials and has no potential environmental risk; the double-layer structure realizes quick-slow synergistic release of nutrients, integrates quick-acting supply of inorganic fertilizers, soil improvement of organic fertilizers and microbial synergistic functions, and can improve the soil structure, enhance the stress resistance of crops and improve the quality and yield of the crops. The process is simple and controllable, complex equipment is not needed, and large-scale popularization is easy.

The application discloses a broad-spectrum growth promoting bacterial agent combination for oil camellia and oil tea, a biological preparation and application thereof, and relates to the field of microbial technology.The endophytic bacteria are separated from healthy oil camellia plants, the abilities of the strains in dissolving phosphorus, fixing nitrogen, producing IAA, producing iron carrier and antagonizing the pathogenic bacteria of oil tea anthracnose are determined, the onion Burkholderia and Aspergillus niger with excellent comprehensive growth promoting functions are screened to prepare a combined bacterial agent, and the combined bacterial agent is verified to have a significant growth promoting effect on the oil camellia and the oil tea through a potting experiment.The combined bacterial agent promotes the growth, nutrient absorption and root development of the oil tea, significantly improves the enzyme activities related to the carbon, phosphorus and nitrogen cycles of the soil, and realizes the overall optimization of the "plant-soil" system.The application finds a broad-spectrum mixed bacterial agent suitable for the artificial planting of the oil camellia and the oil tea in a rocky desertification area, effectively improves the adaptability of the plants in the rocky desertification habitat, and provides a new microbial technical approach for the collaborative repair of multiple tree species in the area.

The present application belongs to the field of traditional Chinese medicine microbial technology, and particularly relates to a method for increasing the content of rapeseed pollen polysaccharide. The method is to increase the content of rapeseed pollen polysaccharide through microbial fermentation, and the microorganism is selected from plant endophytic bacteria and known microbial flora. The microorganism can be a single flora or a mixed flora. The microbial fermentation method can significantly increase the content of plant medicine polysaccharide, create a product probiotic environment, improve the pharmacological activity, and can be widely used in the deep fermentation production of plant medicines.