258 results about "Endophyte" patented technology

The invention discloses bacillus velezensis capable of tolerating various environmental pollution and promoting growth and application of the bacillus velezensis. A plant endophyte bacillus velezensis L381 strain is identified in wild rice, and the strain is high in environmental adaptability, can grow in the environment with the temperature of 15-40 DEG C and the pH value of 5-8, and can tolerate the NaCl concentration of 4%; the rice growth promoting agent has a good growth promoting function, can secrete auxin IAA, solubilize phosphorus, dissolve potassium and secrete siderophores, and can effectively promote rice growth. Meanwhile, the strain has stress resistance to environmental stress such as heavy metals and antibiotics, and tetracycline can be degraded; the strain has a wide antibacterial spectrum, and can be used as a biocontrol bacterium for preventing and treating various plant diseases. The plant endophyte L381 can adapt to variable temperature conditions and variable soil environments in the natural environment, has very strong practical application potential, not only improves the functions of bacillus velezensis, but also widens the application range of the strain.

The invention provides a rice endophyte for promoting rice growth and reducing cadmium as well as a preparation method and application of the rice endophyte, and relates to the field of environmental microbiology and agricultural microorganisms. The rice endophyte is stenotrophomonas, is named as Stentrophomonas sp.R5, is preserved in the China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the postcode is 430072, the preservation number is CCTCC NO: M 20231758, and the preservation date is September 20, 2023. The rice endophyte not only has the capabilities of producing IAA, dissolving phosphorus, producing siderophores and the like for promoting the growth and development of rice, but also has an efficient inhibition effect on absorption and transportation of cadmium in the rice, particularly, the cadmium reduction amplitude of mature rice grains reaches up to 57.69%, and the rice endophyte has little influence on microbial communities in a soil environment, and has a good application prospect. Excessive interference is not caused, and conversion of available nutrients in the soil is promoted.

The invention provides bacillus velezensis NR10, and the preservation number of the bacillus velezensis NR10 is CGMCC (China General Microbiological Culture Collection Center) NO.31771. The bacillus velezensis NR10 is a strawberry endophyte, has good bacteriostatic activity, has good antagonistic action on botrytis cinerea, fusarium oxysporum, colletotrichum gloeosporioides, colletotrichum oxysporum, trichoderma harzianum, trichoderma longibrachiatum and the like, is a potential biological preparation for preventing and treating strawberry diseases and the like, and has broad application prospects. And the bacteriostatic action of the bacillus velezensis NR10 has thermal stability. In addition, the bacillus velezensis also has good capacities of producing siderophores, fixing nitrogen and dissolving potassium, producing protease and cellulase and the like. The bacillus velezensis NR10 provided by the invention has wider-spectrum disease resistance, wide development space and good development and application prospects.

The invention discloses a complex microbial inoculant for promoting growth of leguminous plants and application of the complex microbial inoculant, and relates to the technical field of fertilizers and biologication.The complex microbial inoculant is prepared from 25-35 parts of a microbial inoculant, 10-20 parts of a biological active agent, 10-15 parts of a biological activator and 30-55 parts of water. The endophyte 3G1 which is screened and separated from rice seeds and can promote the growth of leguminous roots can be completely and successfully planted in plants by utilizing the effects of preventing and treating plant diseases and / or promoting plant growth of microorganisms, and the growth of the plants is directly influenced; in combination with the functions of low temperature resistance, plant disease resistance and the like of other complex microbial inoculants, the compound microbial inoculants have growth promoting and disease preventing effects on leguminous plants; the biological active agent is lipopeptide, so that the spreading performance of plant leaves can be improved, the absorption of the complex microbial inoculant is promoted, the activity of the microbial inoculant is improved, and the crop yield is promoted to be increased by 3%; the biological activator can provide trace elements in the plant growth process, and is matched with the complex microbial inoculants for use, so that the growth of crop seedlings can be effectively promoted, the stress resistance of the crop seedlings is improved, and the transplanting survival rate of the seedlings reaches up to 98%.

The invention belongs to the field of microorganisms, and particularly relates to an endophytic bacterium for producing isoamyl alcohol and 3-hydroxy-2-butanone and application of the endophytic bacterium. The name of the endophytic bacterium is Raoultella planticola HuaYu-2, the preservation number of the endophytic bacterium is GDMCC No: 65269, and the endophytic bacterium is preserved in the Guangdong Microbial Culture Collection Center on October 15, 2024. The endophytic bacteria are separated from perfume lemons and can generate endophytic bacteria strains of aroma compounds isoamyl alcohol and 3-hydroxy-2-butanone through fermentation, on one hand, a strain library for fermentation production of perfume compounds is enriched, and on the other hand, a certain basis is provided for screening aroma-producing microorganisms from perfume plants.

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention discloses a gradient internal reference-based absolute quantification method for plant endophyte bacteria, which comprises the following steps of: designing a specific primer for amplifying a plant endophyte bacteria absolute quantification internal reference sequence of a part matched with conserved sequences on two wings of an internal reference sequence; the method comprises the following steps: obtaining an internal reference sequence for amplifying absolute quantification of plant endophyte bacteria and increasing conserved sequences on two wings, and a specific primer for amplifying the plant endophyte bacteria 16SV5V7, mixing the series of internal reference sequences according to a certain gradient proportion, and jointly amplifying and building a library together with total DNA of a plant tissue sample, so as to obtain a specific primer for amplifying the plant endophyte bacteria 16SV5V7. The abundance of the endophyte flora in the plant tissue sample is absolutely quantified, so that the condition of quantitative distortion in plant endophyte detection is eliminated.

This invention relates to compositions and methods for improving plant health, wherein a plant is heterologously disposed to one or more endophytes, or derived from a plant element heterologously disposed to one or more endophytes.

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of Pseudomonas poae which provides bioprotection and / or biofertilizer phenotypes to plants into which it is inoculated. The present invention also relates to plants infected with the endophyte and related methods.

The invention belongs to the technical field of microorganisms, and discloses a compound microbial agent and application thereof in crop disease control. According to the invention, two plant endophytes, namely trichoderma asperellum and trichoderma atroviride, are separated, the trichoderma asperellum is Trichoderma asperellum M11, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.40752; the trichoderma atroviride is Trichoderma atroviride T5, and the preservation number of the trichoderma atroviride is CGMCC (China General Microbiological Culture Collection Center) No. 22432. Fermentation liquor of two trichoderma strains and auxiliaries are prepared into the compound microbial agent, the compound microbial agent has excellent prevention and treatment effects on pepper phytophthora blight, cucumber fusarium wilt, watermelon fusarium wilt, cucumber damping off, tomato sesame spot, tomato early blight and tomato leaf mold, in addition, the compound microbial agent can promote growth and increase yield, the disease resistance of plants is improved, and the application prospect is wide.

The invention discloses endophyte Schizophyllanthus commune CY01-1H for improving synthesis of jasmonic acid and dendrobine of dendrobium nobile seedlings and application of the endophyte Schizophyllanthus commune CY01-1H, and belongs to the technical field of plant secondary metabolism regulation and control and microorganisms. The preservation number of the endophyte CY01-1H is CGMCC (China General Microbiological Culture Collection Center) No.42003; the strain is preserved in China General Microbiological Culture Collection Center on May 12, 2025, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the endophytic fungus CY01-1H obtained through separation comes from a healthy dendrobium nobile plant, the content of jasmonic acid of dendrobium nobile and accumulation of dendrobine can be increased by planting the endophytic fungus CY01-1H in dendrobium nobile, the normal growth state of the plant is not affected, and wide application prospects are brought for improving the quality of the dendrobium nobile medicinal material.

Endophyte combinations, compositions, and methods of using the endophyte combinations and compositions for improving plant and algae productivity and tolerance to environmental stresses.

Disclosed are endophyte inoculant compositions, methods of making the compositions, methods of using the compositions, and physiologically altered plants treated with the compositions. The endophyte inoculant compositions may include one or more of endophyte strains WW5, WW6, WW7, and PTD1, which when applied to a non-native host plant, promote plant mineral nutrient acquisition and uptake, plant vigor, health, growth, and yield.

The invention relates to the technical field of soil carbon improvement, and discloses a composite biological stimulant for tropical rainforest soil carbon improvement and a preparation method and application thereof, the composite biological stimulant is obtained by mixing a microbial preparation, an immobilized carrier, porous biochar, an inorganic mineral compound, a slow release fertilizer, a soil conditioner and a plant symbiotic inoculant; the microbial preparation comprises one or more of lactic acid bacteria, nitrifying bacteria, phosphate solubilizing bacteria, nitrogen-fixing bacteria and arbuscular mycorrhizal fungi; the inorganic mineral compound comprises feldspar, apatite and montmorillonite; the slow-release fertilizer comprises biochar, humic acid and phosphate; the soil conditioner comprises biological colloid prepared from seaweed extract and natural plant fiber prepared from straw and palm fiber; the plant symbiotic inoculant comprises rhizobium and / or endophyte. According to the composite biological stimulant, the soil carbon fixing capacity of the tropical rainforest is remarkably improved, the soil void structure is improved, and the plant growth capacity is enhanced.

The invention provides an endophyte Gliocladium roseum NG-12, which is named as Gliocladium roseum NG-12, and the preservation number of the endophyte Gliocladium roseum NG-12 is CGMCC (China General Microbiological Culture Collection Center) No. 41801. The endophyte Gliocladium roseum NG-12 provided by the invention has good root-knot nematode killing activity and antibacterial activity, has a good antagonistic effect on various plant pathogens such as fusarium oxysporum, colletotrichum gloeosporioides and colletotrichum gloeosporioides, is a potential biological preparation for preventing and treating root-knot nematode diseases, fusarium wilt, anthracnose and the like, and also has the effects of dissolving potassium, fixing nitrogen, dissolving phosphorus, preventing and treating the root-knot nematode diseases and the like. The capability of producing siderophore, protease and cellulase is realized. The gliocladium roseum provided by the invention has a wide development space and a good development and application prospect.

This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and / or seedlings (e.g., cereal seeds and / or seedlings) with heterologous seed-derived bacterial endophytes.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

The invention discloses a method for in-vitro induction of tulip tree cluster buds and regeneration seedlings. The method comprises the following steps: firstly, cutting 3-4cm of a plant material containing terminal buds or axillary buds from a liriodendron twig, disinfecting, removing plant endophyte, then cutting 0.5-1cm of the terminal buds or axillary buds as explants, then putting the explants on a cluster bud induction culture medium for induction culture for about 30 days, then cutting off the induced cluster buds, and putting the induced cluster buds on an elongation culture medium for culture; when the buds extend to be rootless seedlings with the length of 2-3 cm, the seedlings are transferred to a rooting culture medium for rooting induction, and the seedlings can root after being cultured in the rooting culture medium for about 40 days. According to the method, terminal buds and axillary buds are used as explants, growth of endophytes is effectively controlled, a cluster bud induction culture medium, a bud elongation culture medium and a rooting culture medium are screened out, an in-vitro regeneration system is established, and technical support is provided for propagation of liriodendron plants.

Disclosed are endophyte inoculant compositions, methods for making such compositions, methods for using such compositions, and physiologically altered plants treated with such compositions. The endophyte inoculant compositions may include one or more of the endophytic species WW5, WW6, WW7, and PTD1, and when applied to a non-native host plant, promote plant mineral nutrient acquisition and uptake, vigor, health, growth, and yield.

The invention discloses an endophyte extract-containing coated fertilizer and a preparation method thereof, the endophyte extract-containing coated fertilizer sequentially comprises a fertilizer, an inner membrane layer on the surface of the fertilizer and a slow-release outer membrane from inside to outside; and the inner membrane layer contains an endophyte extract. The preparation method of the coated fertilizer containing the endophyte extract comprises the following steps: step 1, preparing a polymer, step 2, adding the endophyte extract into the polymer, step 3, putting fertilizer particles into a coating machine, carrying out rotary preheating, adding the polymer containing the endophyte extract into the surface of the preheated fertilizer particles, and carrying out coating treatment to form an inner layer film; and adding a polyol substance and a polyisocyanate substance for reaction to form a slow-release outer membrane.

The present invention discloses a bipolaris sorokiniana strain SZN‑1 and its application, wherein the strain SZN‑1 is preserved in the China Center for Type Culture Collection, and is classified and named as: Bipolaris sorokiniana SZN‑1, with a preservation number of: CCTCC NO: M2024468, and a preservation date of: March 11, 2024. The present invention separates and screens out a strain bipolaris sorokiniana strain SZN‑1 having a strong inhibitory effect on fusarium wilt from materials such as roots, stems, leaves, flowers, and fruits of fusarium wilt, and finds that it has a preventive and therapeutic effect on plant wilt. The present invention provides new possibilities for the green and effective prevention and control of fungal diseases such as fusarium wilt of fusarium wilt, and also lays a foundation for the further development and utilization of endophyte resources of fusarium wilt in biological pesticides, which has important practical significance for the sustainable development of the fusarium wilt industry.

The present invention discloses a strain of Nocardia YINM00009 and its application in the preparation of cyclic lipopeptide compounds, belonging to the field of microbial technology. The Nocardia YINM00009 is an endophyte of Polygonatum daturae in Kunming, Yunnan Province, and can metabolize a large number of novel cyclic lipopeptide compounds after fermentation; the fermentation step mainly includes: fermenting the strain under suitable culture conditions to obtain a fermentation broth, after solvent extraction and concentration, combined with column chromatography, 9 cyclic lipopeptide compounds can be prepared, the total concentration of the 9 cyclic lipopeptide compounds in the fermentation broth can reach 10.2~16.5mg / L, and the purity after extraction and separation can reach more than 99%, of which 2 cyclic lipopeptide compounds have strong anti-tumor cytotoxic activity. The method is low in cost, simple in process, and environmentally friendly, and can be used for the mass production and preparation of such cyclic lipopeptide compounds, which not only meets the needs of a low-carbon economy, but also lays the foundation for the production of cyclic lipopeptide compounds and the development of related products in the later stage.

The invention relates to the technical field of agricultural planting, in particular to a method for promoting rice growth by using metarhizium anisopliae as rice endophyte, which comprises the following steps: step 1, preparing metarhizium anisopliae spore suspension with the concentration of 1 * 10 * 8 / mL; step 2, soaking rice seeds in the spore suspension for 12 hours; 3, the treated seeds are subjected to soil culture or water culture, and the culture conditions are that the day and night temperature is set to be 26 DEG C, and the photoperiod is set to be 16 L: 8 D; and 4, colonizing the metarhizium anisopliae in root, stem and leaf tissues of the rice to promote the growth of the rice and enhance the resistance to brown planthopper and spodoptera frugiperda. According to the invention, various growth indexes of rice can be obviously improved, and the highest synergism of plant height, root length, fresh weight and dry weight under the soil culture condition is 37.9%, 10.2%, 22.7% and 19.9% respectively; under the water culture condition, the contents are respectively 46.0%, 14.8%, 50.6% and 13.0%.

The present invention relates to the delivery of a methane inhibitor to a plant or a grazing animal. This is achieved by infecting grass with an endophyte which has been modified to produce the methane inhibitor in planta. The endophyte produces the methane inhibitor in planta, which may be consumed by the grazing animal.

The present invention discloses a seed endophyte for preventing and treating wheat fungal diseases and its application, belonging to the technical field of crop planting and crop disease biological control. The wheat endophyte (Pseudescherichia vulneris) GSE25 in the present invention was deposited in the China General Microbiological Culture Collection Center (CGMCC) on November 21, 2022, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC NO.26158. The GSE25 strain has the ability to significantly reduce the pathogenicity of gibberellins and stripe rust on wheat plants, and can significantly reduce the mycotoxins produced by pathogens on wheat grains, has a promoting effect on crop growth and development, and has a broad-spectrum effect. The GSE25 strain of the present invention is of great value in the healthy growth, yield increase, control of pathogenic fungi and mycotoxin pollution of crops.

The invention belongs to the technical field of microorganisms, and particularly relates to a kelp endophyte, i.e., presteria megaterium H403 and an application of the kelp endophyte, i.e., presteria megaterium H403. In order to solve the problems that the kelp seedling raising period is long and the cost is high, an endophytic bacterium is separated from kelp and is named as presteria megaterium H403, a co-culture experiment shows that the strain can remarkably improve the germination rate and the growth rate of kelp seedlings, and the length of kelp in a treatment group added with 14 * 10 < 6 > cfu bacterial liquid is 3.6 times that of a control group. The invention discloses a molecular mechanism for promoting the growth of the kelp through multiple ways by virtue of the Pristeria megaterium H403, a theoretical basis is provided for developing a microorganism-assisted seedling raising technology, the kelp culture cost is expected to be reduced, and the sustainable development of the industry is promoted.

The invention relates to the technical field of peucedanum praeruptorum planting, and discloses a method for improving the quality of peucedanum praeruptorum based on peucedanum praeruptorum endophyte-restrictive penicillium, which comprises the following steps: step 1, separating a sample plant with high abundance from the root of peucedanum praeruptorum, separating restrictive penicillium from the sample plant, performing metabonomics analysis on the restrictive penicillium, and culturing; and 2, preparing a bacterium solution of restrictive penicillium, and inoculating the bacterium solution back to the root of peucedanum praeruptorum by using a root irrigation method. In the step 1, endophyte-restrictive penicillium is obtained from the root of peucedanum praeruptorum. According to the method disclosed by the invention, various coumarin compounds can be generated by tieback of the bacterial liquid of the restrictive penicillium, and bolting and coumarin accumulation of peucedanum praeruptorum can be influenced after tieback. According to the method, restrictive penicillium has an inhibiting effect on bolting of peucedanum praeruptorum dunn and has a promoting effect on coumarin accumulation, the quality of peucedanum praeruptorum dunn can be improved by applying bacterial liquid, and good economic benefits are achieved.

The application discloses application of a bacteria-sugar composition in stress resistance regulation of saline-alkali soil and belongs to the technical field of plant growth regulation. The microorganism is Bacillus, and the plant source extract is a salvia miltiorrhiza-safflower composite polysaccharide. The biological preservation number of the Bacillus in the composition is CGMCC No. 25566, the bacterial concentration is 8.5*10 5 CFU / mL, and the salvia miltiorrhiza-safflower composite polysaccharide is prepared by mixing salvia miltiorrhiza and safflower as raw materials according to a mass ratio of (10~1):(1~10) and adopting polysaccharide extraction, and the concentration is 1 ug / mL. The Bacillus with the biological preservation number of CGMCC No. 25566, which is screened from medicinal plant salvia miltiorrhiza symbiotic microorganism, is combined with the salvia miltiorrhiza-safflower composite polysaccharide to obtain a bacteria-sugar composition for regulating crop tolerance to moderate to severe saline-alkali soil, and the application of salvia miltiorrhiza endophyte and plant source polysaccharide is further developed.

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of Erwinia gerundensis which provides bioprotection and / or biofertilizer phenotypes to plants into which it is inoculated. The present invention also discloses plants infected with the endophyte and related methods.