78 results about "Bacillus atrophaeus" patented technology

The invention relates to the technical field of Bacillus, particularly a salt-tolerant Bacillus strain. The Bacillus is Bacillus atrophaeus. The Bacillus atrophaeus strain is selected from a strain named Ba10, the Ba10 is collected by China General Microbiological Culture Collection Center, and the collection number is CGMCC No.12053; and/or the Bacillus atrophaeus strain is selected from a strain named Ba27, the Ba27 is collected by China General Microbiological Culture Collection Center, and the collection number is CGMCC No.12054. The Bacillus atrophaeus strain can inhibit multiple pathogens from growth, and has tolerance to high-concentration salts.

The invention relates to a micro-capsulated suspended microbial seed coating agent and a preparation method thereof. The micro-capsulated suspended microbial seed coating agent comprises a functional bacteria microcapsule and auxiliaries, wherein the functional bacteria microcapsule is prepared from wall materials such as sodium alginate and gelatin by virtue of an endogenous emulsion process, the grain size of the microcapsule is 20-60 mu m, the content of functional bacteria is not lower than 10<9>cfu/g, and the embedding rate is over 80%. Functional microbes are selected from two or more of pseudomonas, bacillus subtilis, bacillus amyloliquefaciens, enterobacter cloacae, raoultella planticola and bacillus atrophaeus. The auxiliaries comprise one or more of a film-forming agent, a dispersant, a tackifier, an anti-freezing agent and a coloring agent. The prepared functional bacteria microcapsule is uniformly mixed with the auxiliaries to obtain a finished product. The product has the advantages of good film-forming performances, thallus persistence, high survivability, good slow-controlled release, strong application stability, environment friendliness, convenience in use, and the like, can be used for promoting seed germination and seedling growth of cottons, soya beans, wheat and potatoes, and the like, controlling the diseases at the seedling stage, improving stress resistance, and the like.

The invention discloses a bacillus atrophaeus strain DPPG-28 and an application in crop disease control, and belongs to the field of agricultural biotechnology. The strain is separated from soil of drip irrigation fields with damping off in tomato processing production areas, and has an accession number of CGMCC No. 9394. The strain can be used for biological control of crop pathogens such as rhizoctonia solani, pythium aphanidermatum, alternaria solani, phytophthora capsici, tomato scab fungi, and the like; the control effects on tomato damping off, capsicum early blight, and cotton damping off reach respectively 74.17%, 66.83%, and 70.12%; the strain can significantly improve the crop germination rate, plant height, fresh weight, and dry weight at the seedling stage. The strain of the invention has high-efficient wide-spectrum germicidal activity, and has wide application prospects in biocontrol agents.

The invention discloses Bacillus atrophaeus and an application thereof. The Bacillus atrophaeus JZB120050CGMCC No.10919 has stable, efficient and broad-spectrum antibacterial properties. The Bacillus atrophaeus can generate a biological membrane, has strong capability of generating siderophore, chitinase, cellulase and protease, which shows that the strain has relatively strong biocontrol potential. The control effect of fermentation liquor of the strain to tomatoes inoculated with botrytis cinerea for culturing 11d is 100% (the control effect of a 5-time diluent treatment group of JZB120050 strain fermentation liquor is 48%, the control effect of a treatment group of JZB120028 strain fermentation liquor is 50%, the control effect of a 5-time diluent treatment group of JZB120028 strain fermentation liquor is 26%, and the control effects of a blank control group, a 1000-time liquid treatment group of 3% polyoxin, and 1000-time liquid treatment group of 50% carbendazim are 0%), which shows that the strain has an effective inhibitory action on the botrytis cinerea. The strain is safe to human and domestic animals, does not have environmental pollution problems, is simple in culture condition, easy to store and suitable for industrial production, and has a good development application prospect.

The invention discloses a multifunctional composite microecological preparation for sea cucumber cultivation, which comprises: Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, and a carrier. The invention screens good symbiosis between probiotics, has good antagonistic effect on sea cucumber pathogenic bacteria, and has the function of improving sea cucumber growth speed, immunity and intestinal digestive enzyme activity, and achieves antibacterial, Multiple functions of immunity and growth promotion. The indigenous bacteria screened from the intestines of sea cucumbers are safe and non-toxic to sea cucumbers, have a fast reproduction speed, are easy to cultivate, have strong adaptability to the environment, and can reproduce quickly to form dominant bacteria. The method of use is simple, and the preparation is only required to be added to the feed according to the ratio for feeding, and is suitable for young ginseng breeding and sea cucumber disease control in various breeding modes. It plays an important role in improving the quality of sea cucumber products and ensuring food safety.

The present invention discloses a strain of Bacillus atrophaeus HMB22922 for prevention and control of cotton boll blight, wherein the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 20, 2011, and the preservation number is CGMCC No.5614. The present invention further discloses a microbial agent produced by using the strain, and a preparationmethod thereof. Active ingredients of the microbial agent are HMB22922 thallus and extracellular metabolites thereof. The HMB22922 strain of the present invention provides special prevention and control effects for cotton boll blight, wherein the prevention and control efficiency is high, and the average prevention and control efficiency is more than 80%. The microbial agent of the present invention is safe for human and animals, and does not cause environmental pollution. Drug resistance is not easily generated when the strain and the microbial agent are adopted to prevent and control the cotton boll blight. In addition, the preparation method for the microbial agent of the present invention has characteristics of simpleness, low cost, and easy use.

The invention discloses a Bacillus atrophaeus strain BsR05 and an application thereof. The Bacillus atrophaeus strain BsR05 is collected in the CGMCC (China General Microbiological Culture Collection Center) with the collection number being CGMCC NO.11665. A Bacillus atrophaeus strain BsR05 liquid suspending agent prepared from the strain has a specific control effect on cucumber gray mold, and the control effect is high and is averagely 70% or higher.

The invention discloses compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is prepared from Bacillus atrophaeus and trichoderma which are taken as main active components, and can be used for controlling fungal diseases such as vegetable gray mold and the like in the field. The Bacillus atrophaeus in the compound microbial fertilizer has an inhibiting action on various plant pathogenic fungi, and meanwhile, the trichoderma LTR-2 has phytase activity, so that the compound microbial fertilizer can be used for controlling various plant diseases caused by the plant pathogenic fungi, has the functions of increasing utilization of phosphorus in soil by plants, improving the soil structure, promoting the plant growth and the like, and is multifunctional.

The invention discloses a strain of antagonistic bacteria for preventing and controlling poplar colletotrichumgloeosporioides and application thereof. The antagonistic bacteria are bacillus atrophaeus XW2, and the microbial collection number is CGMCC NO. 7689. The bacillus atrophaeus XW2 is obtained through plate face-to-face culturing and screening of poplar endophytes via a flat plate, has an obvious antagonistic effect on the poplar colletotrichumgloeosporioides, can be used for effectively inhibiting the growth of poplar colletotrichumgloeosporioides hypha and germination of spores and preventing and controlling plant diseases caused by the poplar colletotrichumgloeosporioides, is a biological prevention and control potential strain with high protection effect and good environmental safety and has good development and application prospect.

The invention belongs to the technical field of gene engineering, and particularly relates to a chitosanase gene csnbaa, chitosanase and a preparation method and application thereof. The nucleotide sequence of the chitosanase gene csnbaa is shown as SEQ ID NO: 1, and the chitosanase gene csnbaa is obtained by comprehensively optimizing the sequence of chitosanase derived from Bacillus atrophaeus.The amino acid sequence of the chitosanase CsnBaa is as shown in SEQ ID NO: 2, so that not only are the types and source ways of chitosanase enriched, but also the fermentation enzyme activity can reach 4630 U/mL, and the chitosanase CsnBaa shows good stability in a relatively wide temperature range and a relatively wide pH range and has good application prospect and industrial value.

The invention relates to antibiological inoculant. The effective active component of the antibiological inoculant is bacillus atrophaeus YL3. The antibiological inoculant is characterized in that the antibiological inoculant is an aqueous solution and is prepared from, by weight, 89.5-99.8% of bacillus atrophaeus YL3, 0.1-10% of sodium dodecyl sulfate and 0.1-0.5% of potassium sorbate. During application, the aqueous solution is used for preventing and treating strawberry anthracnose and strawberry powdery mildew when sprayed on leaf surfaces and used for preventing and treating various types of crop wilt and the like when irrigated to roots. The antibiological inoculant has the advantages that by introducing bacillus atrophaeus YL3 which is safe to humans and livestock and has a good effect on preventing and treating diseases of strawberries and various plants, and the antibiological inoculant can prevent and treat plant diseases through biological agents; the dosage of chemical agents is reduced; meanwhile, the biological environment is improved, and the amount of chemical agents left in agricultural products is decreased.

The invention discloses a bacillus atrophaeus strain Aj080319IA-12. The bacillus atrophaeus strain is characterized by being separated from an intestinal tract of a wild stichopus japonicus selenka and collected in China Center for Type Culture Collection (CCTCC) along with a collection number of CCTCC M 2010316. The screened bacillus atrophaeus has remarkable antagonistic action on pathogenic bacteria of a sea cucumber, and has the effects of increasing the growth speed, immunity and intestinal digestive enzyme activity of the stichopus japonicus selenka. The screened bacillus atrophaeus is indigenous bacterium screened from the intestinal tract of the sea cucumber, which is safe and non-toxic for the sea cucumber, fast in breeding speed, easy to culture and strong in adaptability on environment, and is capable of being bred quickly to form predominant bacteria. A microbiological preparation, as a feed additive, can increase the specific growth rate of the sea cucumber, reduce the death rate of the sea cucumber, improve the immunity of the sea cucumber and the digestive enzyme activity of the intestinal tract, and improve the disease resistance of the sea cucumber at the same time, so that the preparation achieves the effects of resisting bacteria, immunizing and promoting growth, and is capable of reducing and replacing the use of antibiotics.

The invention relates to the fields of biopesticide, and specifically discloses a Brevibacillus atrophaeus wettable powder containing a spore germinator, and a preparation method thereof. The wettable powder consists of the following components by weight: 40-70 parts of a Brevibacillus atrophaeus fermentation broth, 20-30 parts of a kaolin filler, 1-4 parts of a wetting agent sodium lauryl sulfate, 0.2-1 part of a dispersant of Morwet D-425, a spore germinator containing 0.1-1 part of fructose, 0.1-1 part of threonine and 0.1-0.5 part of pyridine-2,6-dicarboxylic acid calcium, a UV protective agent containing 1-2 parts of mycose and 0.2-1 part of carboxymethyl cellulose, and a drying protecting agent containing 1-5 parts of cane sugar and 0.2-1 part of monosodium glutamate. After application of the Brevibacillus atrophaeus wettable powder into the field, the spores have high germination speed and high germination rate; and the control effect of the Brevibacillus brevis wettable powder can be significantly improved, the usage amount of spores and the production costs can be reduced.

A process for obtaining gold magnetic nanoparticles or of other metallic elements by fermenting a culture medium in the presence of producer microorganisms such as Brevibacterium halotolerans, Bacillus mojavensis, Bacillus subtilis subsp. Inaquosorum, Bacillus subtilis subsp. Spizizenii, Bacillus tequilensis, Bacillus amyloliquefaciens subsp. Amyloliquefaciens, Bacillus siamenensis, Bacillus amyloliquefaciens subsp. Plantarum, Bacillus subtilis, Bacillus subtilis subsp. Inaquosorum, Bacillus atrophaeus, or Bacillus vallismortis, inter alia. The process of the invention can be used to effectively control the size and shape of the nanostructure to be obtained, with production levels above those found at laboratory level.

The invention provides bacillus atrophaeus BA-7 used for preventing and treating clubroot of cruciferae crops. The bacillus atrophaeus BA-7 is characterized in that the classification name of the bacillus atrophaeus is Bacillus atrophaeus BA-7, and the bacillus atrophaeus BA-7 is preserved in the China Center for Type Culture Collection (CCTCC) on Dec 21, 2015, and is assigned with the accession number of CCTCC NO:M2015765. Under the low concentration, the bacterial strain has the obvious prevention and treatment effects for the clubroot of the cruciferae crops, and the prevention and treatment effects of a 1000-fold diluent (obtained after culture for 24h) for the clubroot of oilseed rape achieve 87.2%

The invention discloses a microbial organic fertilizer, which is prepared from the following steps: inoculating Bacillus atrophaeus QHZ-3 having a preservation number of CGMCC No.13639 in a LB culture liquid, and carrying out liquid fermentation to obtain a QHZ-3 bacterial liquid having the effective viable count of more than or equal to 1*10<9> CFU/mL; preparing an amino acid organic fertilizer, wherein an animal source amino acid stock solution is added to an organic fertilizer according to a ratio of 15 ml/100 g, and fermenting is performed for 5 d to achieve the pH value of 6.5-6.8; and inoculating the QHZ-3 bacterial liquid in the amino acid organic fertilizer according to a ratio of 5 ml/100 g, and carrying out secondary solid fermentation, such that the effective viable count of the QHZ-3 is more than or equal to 0.2*10<9> cfu/g, and the water content is evaporated to less than 30%. According to the present invention, the microbial organic fertilizer has antagonistic effect on Rhizoctonia solani, and can effectively promote the growth of potato plants and increase the tuber yield.

The invention provides a method for preventing and controlling a clubroot disease of cruciferous crops by means of bacillus atrophaeus BA-7. The method comprises any one of the following methods that 1, root irrigation treatment is conducted on the cruciferous crops at least three times with bacillus atrophaeus BA-7 liquid on the first day after sowing, the seventh day after sowing and the fifteenth day after sowing, wherein 80-100 ml of the bacillus atrophaeus BA-7 liquid is applied to each plant every time; 2, spraying treatment is conducted on the cruciferous crops at least three times with bacillus atrophaeus BA-7 liquid on the first day after sowing, the seventh day after sowing and the fourteenth day after sowing, wherein 150-200 ml of the bacillus atrophaeus BA-7 liquid is applied to every square meter of the crops. The cruciferous crops comprise oilseed rapes, Chinese cabbages and broccoli; the classification name of the bacillus atrophaeus is bacillus atrophaeus BA-7, the bacillus atrophaeus BA-7 is preserved in the China Center for Type Culture Collection on December 21, 2015, and the preservation number is CCTCC NO:M 2015765.

The invention relates to a bacterial strain, a microbial fertilizer containing the bacterial strain, and a preparation method and a use of the microbial fertilizer. The microbial fertilizer is prepared by reasonable combination and blending of a bacillus atrophaeus strain agent, fulvic acid, monopotassium phosphate, phosphoric acid, zinc sulfate, ferrous sulfate, ammonium molybdate, copper sulfate and manganese sulfate as raw materials. The microbial fertilizer can improve salinity soil, has effects of salt decomposition and growth promotion of a plant, and adjustment of a structure and a pH value of soil, also has high fertilizer efficiency and a long quality guarantee period, and solves the problems of saline-alkali soil harm, a short quality guarantee period and poor fertilizer efficiency.

Bacillus atrophaeus CPB072 having poisoning and controlling effects on potato beetles is separated from natural environment, can grow and massively produce spores under conventional culture conditions, an optimum culture medium is a Luria-Bertani culturing medium, an optimum temperature is 30DEG C, and an optimal pH value is 7.0; and an optimum condition of fungal infection pathogenicity is that the inoculation concentration is more than 10<8>/mL. The above bacterium has strong pathogenic poisoning effects on potato beetle larvae, has strong insecticidal activity on lepidopteran insects, can be processed through a bacterium fermentation technology to produce a safe and environmentally-friendly bacterial pesticide without pollution or residual, can also clone other insecticidal functional genes for research and development of transgenic beetle-resistant potato, and has latent commercial development and application values.

The invention discloses a microbial compound fungicide for degrading waste gas of a rubber plant and a preparation method and application thereof. The compound fungicide comprises, by mass, 5-10 partsof bacillus atrophaeus, 5-10 parts of empedobacter brevis, 2-5 parts of photobacterium beijerinck, 2-5 parts of pseudomonas alcaligenes, 2-5 parts of virgibacillus pantothenticus and 5-10 parts of pseudomonas putida. The microbial compound fungicide can be applied to degradation of the waste gas of the rubber plant, the degradation effect is good, the culture cost is low, the microbial compound fungicide is easy and convenient to use, the VOCs removal rate is 90% or above, and the national emission standard is met.

The invention discloses a calibration bin, calibration system and method for the sampling physical efficiency of an air microorganism sampler. The calibration bin comprises an aerosol chamber, a clean air system, an environment temperature and humidity adjustment module, an environment parameter monitoring module, a negative pressure adjustment module, a turbulent flow fan set, a cleaning, disinfection and sterilization module, a waste gas treatment module, an upper computer monitoring module, an an aerosol generating interface. The calibration system comprises the calibration bin for the sampling physical efficiency of the air microorganism sampler, a monodisperse aerosol generator, an air particulate matter particle counter, a membrane filtration air microorganism sampler comparator and a potassium iodide ethanol solution containing bacillus atrophaeus standard substances for calibration. According to the invention, the problem that no calibration device is provided for the sampling physical efficiency of the existing air microorganism sampler is solved, and the calibration of the sampling physical efficiency of five bacteria-containing particles with the particle size of 0.7-15 [mu]m generated by the monodisperse aerosol generator can be realized.

The invention relates to construction of a recombinant bacterium for efficiently expressing chitinase and screening of a mutant with high enzyme activity. According to the invention, efficient secretory expression of BcchiA1 in bacillus subtilis is realized by means of a recombinant protein technology, and the problem of insufficient chitinase secretion amount of most microorganisms is solved. Meanwhile, a novel chitinase high-throughput screening method is created, and the screening throughput and efficiency are greatly improved. In addition, the invention relates to several strains of chitinase mutants with high enzyme activity, the enzyme activities of BcchiA1 (Y10A), BcchiA1 (R301A), BcchiA1 (E327A), BcchiA1 (Y10A/R301A), BcchiA1 (Y10A/E327A), BcchiA1 (R301A/E327A) and BcchiA1 (Y10A/R301A/E327A) are respectively improved by 2.49 times, 0.67 times, 3.61 times, 2.39 times, 3.46 times, 4.75 times and 16.89 times compared with the enzyme activities of the wild type BcchiA1, and the enzyme activity of BcchiA1 (Y10A/R301A/E327A) is optimal. Finally, after the chitinase BcchiA1 and the optimal mutant BcchiA1 (Y10A/R301A/E327A) of the chitinase BcchiA1 and the monooxygenase BatLPMO10 from bacillus atrophaeus are subjected to a synergistic effect respectively, the hydrolysis efficiency of the chitinase BcchiA1 can be improved by 50.00%, and the hydrolysis efficiency of the chitinase BcchiA1 (Y10A/R301A/E327A) can be improved by 46.71%.

The invention discloses a sterilizing composite of bacillus atrophaeus and lentinan. The sterilizing composite comprises effective ingredients of bacillus atrophaeus and lentinan, wherein the content of bacillus atrophaeus is one hundred million to five billion CFU/g; the mass percent of lentinan is (0.1-1.5)%. The sterilizing composite is prepared by compounding bacillus atrophaeus and lentinan and can be used for preventing and treating tobacco bacterial wilt disease and virus disease of tobacco, and the sterilizing composite has good preventing and treating effects; meanwhile, the sterilizing composite can improve the tobacco resistance and improve the tobacco yield and quality, and moreover, the sterilizing composite is safe to human, animal, beneficial organisms and environment.

The invention provides a fermentation method of bacillus atrophaeus BA-7 capable of preventing and treating clubroot of cruciferae crops. The fermentation method comprises the following steps: (1) culturing the bacillus atrophaeus BA-7 in a solid medium, picking single colonies, placing the single colonies in a fluid medium, carrying out shake culture for 10-12 h at the temperature of 30-32 DEG C, thus obtaining the seed solution, wherein the rotation speed is 200-220 rpm; (2) after inoculation, carrying out fermentation for 24-48 h, wherein the temperature of the tank is 28-32 DEG C, the stirring speed of the fermentation tank is 220-250 rpm, the culture medium in the fermentation tank is prepared from the following components in percentage by weight: 4-5 % of soya flour, 0.1-0.15 % of peptone, 0.5-0.7 % of glucose, 2-3 % of starch, 0.1% of manganese sulfate, 0.06% of ferrous sulfate, 0.2% of dipotassium phosphate, 0.1% of calcium carbonate, 0.01% of a defoamer, the balance of water, and the pH is 7.0-7.4. The classification name of the bacillus atrophaeus is bacillus atrophaeus BA-7, and the bacillus atrophaeus BA-7 is preserved in the China Center for Type Culture Collection (CCTCC), and is assigned with the accession number of CCTCC NO:M 2015765.

The invention discloses Bacillus atrophaeus E20303 and application thereof. The Bacillus atrophaeus E20303 has been preserved in Guangdong Microbial Culture Collection Center under the preservation number GDMCC No: 60695 since June 17, 2019. The Bacillus atrophaeus E20303 is capable of effectively inhibiting potato diseases caused by fusarium solani and potato virus Y. The Bacillus atrophaeus E20303 is a moderately halophilic bacterium which is isolated and purified from the water of Qarhan Salt Lake in Qinghai Province, and provides a new way for controlling potato diseases caused by fusariumsolani and potato virus Y; and moreover, the Bacillus atrophaeus E20303 is capable of effectively improving the problem of failure to achieve prevention and control effects caused by selective resistance of pathogens. Therefore, the Bacillus atrophaeus E20303 has good prospects of application.