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Bacillus atrophaeus is a species of black-pigmented bacteria. Its type strain is NRRL NRS-213. B. atrophaeus strains have been used extensively in biomedicine as indicator strains for heat- and chemical-based decontamination regimens. Most of the strains in use are derivatives of a lineage of B. atrophaeus that originated at Camp Detrick in the 1950s, where many modern biocontainment procedures were developed. B. atrophaeus has historically been known by several other names, including B. globigii (the origin of its military moniker "BG") and B. subtilis var. niger. Modern phylogenetic analyses using multiple genetic methods have placed B. atrophaeus close to B. subtilis. Its original and still most prominent use is as a surrogate organism for pathogenic B. anthracis, beginning in the U.S. bio-weapons program, as its pigmentation readily facilitated discrimination from non-pigmented background organisms in environmental samples. Subsequent genomic and phenotypic analysis of strains derived from the Camp Detrick isolates revealed that they had been deliberately selected for strains that exhibited elevated rates of sporulation.
Saline-alkali tolerant strain capable of producing IAA and application thereof
The invention discloses and relates to a strain capable of resisting saline and alkaline and producing IAA (Indoleacetic Acid). The strain is classified and named as bacillus atrophaeus, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.29799. The strain is preserved in China General Microbiological Culture Collection Center on January 29, 2024. The bacillus atrophaeus HHQGTS13-1 can grow under the condition that the salt content is higher than 3%, can generate indoleacetic acid, cellulase, amylase, protease and ligninase, can inhibit various plant pathogenic bacteria, such as banana anthracnose, tomato gray mold, cotton verticillium wilt, rice sheath blight disease, wheat head blight, banana fusarium wilt, capsicum white spot and the like, and can be used for preparing the bacillus atrophaeus HHQGTS13-1. Particularly, the compound plays an important role in preventing and treating wheat scab, banana anthracnose and capsicum white spot. The strain can promote plant growth in a saline-alkali environment, and can be used for improving soil quality, developing special microbial fertilizers, microbial agents and biopesticides for saline-alkali soil, relieving crop salt stress damage and increasing crop yield.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP
Bacillus atrophaeus for preventing and treating wheat gibberellic disease caused by gibberellic maize and application of bacillus atrophaeus
The invention relates to the technical field of biological control of plant diseases, in particular to application of a bacillus atrophaeus strain and a preparation thereof to control wheat gibberellic disease caused by gibberellic maize. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.29799, and the strain is classified and named as the bacillus atrophaeus strain HHQGTS13-1 (Bacillus atrophaeus HHQGTS13-1). The bacillus atrophaeus strain HHQGTS13-1 is wide in application range to natural environment conditions such as temperature and pH, and has the capability of antagonizing fusarium maydis when the temperature is 10-45 DEG C and the pH value is 4-10. The bacillus atrophaeus strain HHQGTS13-1 can be used for obviously inhibiting the growth of fusarium maydis hyphae and enabling the hyphae to be deformed; the antagonistic bacillus HHQGTS13-1 strain has an obvious inhibition effect on spore germination of fusarium maydis, the spore germination inhibition rate is 97.10%, the prevention and treatment effect of the antagonistic bacillus HHQGTS13-1 strain fermentation liquor after wheat is inoculated with fusarium maydis pathogenic bacteria is 63.43%, and the antagonistic bacillus HHQGTS13-1 strain has good potential of preventing and treating plant diseases caused by fusarium maydis.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP +2
Microbial complex microbial inoculant, preparation method thereof and application of microbial complex microbial inoculant in prevention and treatment of potato scab
The invention relates to the technical field of microbial agents, and discloses a composite microbial agent, a preparation method thereof and application of the composite microbial agent in prevention and treatment of potato scab. Wherein the microbial complex microbial inoculant is prepared from bacillus aglaia pairwise 6096, streptomyces sp. 6219 and bacillus atrophaeus 4618; the aglaia odorata pairwise bacillus 6096, the streptomyces 6219 and the bacillus atrophaeus 4618 are all preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the aglaia odorata pairwise bacillus 6096, the streptomyces 6219 and the bacillus atrophaeus 4618 are respectively CGMCC NO.34691, CGMCC NO.34692 and CGMCC The composite microbial inoculant is good and stable in antibacterial effect, and the morbidity and disease index of potato scab can be remarkably reduced after the composite microbial inoculant is used.
Owner:INNER MONGOLIA UNIVERSITY
Microbial composite agent, preparation method thereof and application in preventing and controlling potato scab
The present invention relates to the technical field of microbial agents, and discloses a microbial composite agent, a preparation method thereof, and its use in preventing and treating potato scab. The microbial composite agent comprises: P. milanoxanus 6096, Streptomyces 6219, and Bacillus atrophaeus 4618; P. milanoxanus 6096, Streptomyces 6219, and Bacillus atrophaeus 4618 are all deposited in the General Microbiology Center of the China National Center for Microbiological Culture Collection, with deposit numbers CGMCC No. 34691, CGMCC No. 34692, and CGMCC No. 34952, respectively. The microbial composite agent of the present invention has a good and stable antibacterial effect and can significantly reduce the incidence and disease index of potato scab after use.
Owner:INNER MONGOLIA UNIVERSITY
Compound microbial agent as well as preparation method and application thereof
The invention relates to the technical field of biological agents, and particularly discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent provided by the invention comprises compound bacillus, a carrier and a coating agent, the composite bacillus comprises bacillus velezensis, bacillus atrophaeus and bacillus firmus with the viable count ratio of (3-7): (1-4): 2; the viable count of the compound microbial agent is greater than or equal to 5 * 10 < 9 > cfu / g; the invention also provides a preparation method of the compound microbial agent. The preparation method comprises the following steps: strain fermentation, carrier adsorption, granulation and enveloping and low-temperature drying. The compound microbial agent provided by the invention can improve the soil structure, enhance the soil fertility and inhibit soil-borne diseases, so that the growth condition of crops is improved, and the quality and yield of the crops are improved.
Owner:BEIJING GOLDENWAY BIO TECH
Multi-strain microbial agents for the prevention and control of potato scab, their preparation methods and applications
This invention relates to the field of microbial inoculants, and discloses a multi-strain microbial preparation for the prevention and control of potato scab, its preparation method, and its application. The multi-strain microbial preparation includes: *Bacillus atrophicus* 6052, *Bacillus compostii* 6069, *Microbacterium natriureticum* 6035, and *Bacillus atrophicus* 4618; all of these strains are deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession numbers CGMCC NO.34693, CGMCC NO.34694, CGMCC NO.34695, and CGMCC NO.34952, respectively. The multi-strain microbial preparation of this invention exhibits good and stable antibacterial effects, and its use can significantly reduce the incidence and disease index of potato scab.
Owner:INNER MONGOLIA UNIVERSITY
A microbial composite bacteria H2 for antagonizing multiple pathogenic fungi of potato, and its application and preparation method
A microbial composite strain H2, antagonistic to multiple potato pathogens, and its application and preparation method, relates to the field of biological control. The microbial composite strain H2 is a mixture of Bacillus subtilis, Bacillus atrophaeus, and Bacillus Widmannii. It exhibits strong inhibitory effects against potato early blight pathogens Alternaria solani and Alternaria alternata; wilt / dry rot pathogens Fusarium solani, Fusarium oxysporum, and Fusarium equiseti; and leaf / stem spot pathogen Vitis vinifera, with inhibition rates exceeding 46%. The highest inhibition rate against Alternaria alternata reached 75.97%. H2 also exhibits significant growth-promoting and yield-increasing effects, providing a high-quality strain for the comprehensive control of soil-borne potato diseases and promising prospects for field application.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Compound bacteria and application thereof in kitchen waste recycling
The invention relates to the technical field of kitchen waste treatment, and particularly discloses compound bacteria and application thereof in kitchen waste recycling. The compound bacteria disclosed by the invention comprise a component A and a component B, the component A is formed by mixing bacillus velezensis, bacillus amyloliquefaciens, pseudomonas, bacillus atrophaeus, brevibacillus laterosporus and trichoderma harzianum; and the component B is prepared by mixing bacillus parlicheniformis, bacillus licheniformis, urea bacillus thermophilus and fat bacillus thermophilus. According to the technical scheme of resourceful treatment on the kitchen garbage by using the compound bacteria provided by the invention, the degradation effect of the kitchen garbage can be effectively improved, and the purposes of reduction, harmlessness and resourceful treatment of the kitchen garbage and energy consumption reduction in the treatment process are achieved; and the kitchen waste resourceful treatment material has an excellent application effect as a soil conditioner and a feed.
Owner:BEIJING GOLDENWAY BIO TECH
A method for promoting production of gamma-polyglutamic acid by bacillus fermentation using an oxygen carrier
This invention relates to the field of microbial fermentation, specifically to a method for promoting the fermentation of Bacillus atrophaeus to produce γ-polyglutamic acid (PGA) using oxygen carriers. This invention involves adding specific alkane oxygen carriers during the initial stage of γ-PGA production via Bacillus atrophaeus NWS-01 fermentation. Hexane, heptane, and hexadecane were selected as effective oxygen carriers, and the optimal single-addition concentration and optimal synergistic addition ratio of each oxygen carrier were determined. This solves the problem of limited γ-PGA yield due to low oxygen transfer efficiency in traditional fermentation processes. Specifically, adding 0.3% heptane alone achieves a γ-PGA yield of 39.4 ± 0.19 g / L, while the synergistic addition of 0.240% hexane, 0.407% heptane, and 1.014% hexadecane results in a maximum yield of 42.1 g / L, representing a 150.6% increase compared to the fermentation system without added oxygen carriers. This method is simple to operate, has high oxygen utilization, low energy consumption, and produces fewer bubbles and lower liquid shear force in the fermentation system, making it suitable for the industrial-scale aerobic high-density fermentation production of γ-PGA.
Owner:XUANKAI BIOTECHNOLOGY (SHANDONG) CO LTD
Sterilization biological indicator and application thereof
PendingCN121065021ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologySporeling
The invention relates to the technical field of biology, in particular to a sterilization biological indicator and application thereof. The invention provides a biological indicator composed of sulfhydrylated spores containing bacillus atrophaeus and a spore culture solution containing a gold nanoprobe. The biological indicator is used for monitoring the sterilization effect, solves the problems of low spore detection speed of bacillus atrophaeus after ethylene oxide sterilization and high use cost of a rapid ethylene oxide indicator, can realize rapid detection within 10 minutes after sterilization, and does not need a complex instrument. The method has a wide application prospect in the monitoring of the sterilization effect of ethylene oxide sterilization equipment.
Owner:SHINVA MEDICAL INSTR CO LTD
Biocontrol bacillus strain for relieving parasitic sunflower of orobanche coerulescens as well as derivative product and application of biocontrol bacillus strain
The invention provides a biocontrol bacillus strain for relieving parasitic sunflower of orobanche coerulescens as well as a derivative product and application of the biocontrol bacillus strain, and belongs to the technical field of biocontrol microorganisms. The invention firstly provides an application of bacillus atrophaeus or a biocontrol microbial inoculum thereof in prevention and control of Orobanche coerulescens parasitic sunflowers, and further provides an application of the Orobanche coerulescens in prevention and control of Orobanche coerulescens. Meanwhile, the invention also provides a method. The strain B45 is prepared into a biocontrol microbial inoculum, the biocontrol microbial inoculum has the advantages of being safe, economical and high in specialization, and the biocontrol microbial inoculum achieves the purpose of preventing and treating orobanche coerulescens by reducing the population density of pests and does not cause pollution to the environment.
Owner:CHINA AGRI UNIV
Bacillus atrophaeus SCAU-H1 capable of repairing heavy metal pollution and application thereof
The present application belongs to the technical field of pollution remediation, and particularly relates to Bacillus atrophaeus SCAU-H1 capable of remediation of heavy metal pollution and application thereof. The present application obtains a strain of Bacillus atrophaeus SCAU-H1, which is preserved in Guangdong Microbial Culture Collection Center on December 22, 2023, with a preservation number of GDMCC NO: 64181. The strain SCAU-H1 can effectively remove As(III), As(V), Cd, Pb and Sb heavy metals in water, and particularly has excellent removal effect on As(V). Moreover, the strain can significantly reduce the effective content of heavy metals Cd and Pb in soil, and has a significant immobilization effect. Therefore, the strain SCAU-H1 can be used for remediation and treatment of heavy metal contaminated water and soil.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
A bacterium-chemical composition for preventing and treating potato scab and application thereof
ActiveCN119867083BImprove the effect of prevention and controlreduce morbidityBiocideBacteriaBiotechnologyChemical control
This invention belongs to the field of potato scab control technology, specifically relating to a fungal-agent composition for controlling potato scab and its application. The fungal-agent composition for controlling potato scab provided by this invention comprises the following raw materials: a chemical control agent for potato scab and a compound fungal agent; the volume ratio of the chemical control agent to the compound fungal agent is 75-150:5000; the compound fungal agent includes *Bacillus belyceta* BEV2 and *Bacillus atrophus* FM2-4. Using the fungal-agent composition of this invention can reduce the dosage of chemical agents by at least half, while providing rapid, long-lasting, and effective control of potato scab, and also taking into account the advantages of environmental friendliness.
Owner:HEBEI AGRICULTURAL UNIV.
Synthetic bacterial flora co-culture fermentation broth, bacterial agent and application thereof
This invention relates to the field of agricultural biological control technology, specifically to a synthetic microbial co-culture fermentation broth, its inoculum, and its application. The synthetic microbial co-culture fermentation broth is prepared by sequential co-inoculation fermentation of *Trichoderma harzianum* TW21990, *Burkholderia viminalis* P418, and *Bacillus atrophicus* BsR05; wherein *Trichoderma harzianum* TW21990 has the CGMCC No. 12864, *Burkholderia viminalis* P418 has the CGMCC No. 1212, and *Bacillus atrophicus* BsR05 has the CGMCC No. 11665. Compared to single biocontrol strains, the antibacterial and growth-promoting secondary metabolites produced by the above-mentioned synthetic microbial co-culture fermentation are broad-spectrum, making it less likely to induce drug resistance in the control of gray mold in leeks. The control effect is stable, there is no antagonism within the synthetic microbial community, and the efficacy of the strains is synergistically enhanced, with a significant growth-promoting effect on leeks.
Owner:BIOTECH CENT OF SHANDONG ACAD OF SCI
Preparation method of high-activity multifunctional foliar bacterial fertilizer
The invention relates to the technical field of agricultural microbial fertilizers, in particular to a preparation method of a high-activity multifunctional foliar bacterial fertilizer, which comprises the following steps: preparing a composite culture medium, and accurately calculating the mass ratio of fish peptone to trehalose based on a target carbon nitrogen ratio; inoculating bacillus atrophaeus, bacillus siamensis and pseudomonas fluorescens in a specific proportion into the culture medium, and performing dynamic fed-batch fermentation; after fermentation, thalli are wrapped by forming protective colloid, and the addition condition of the microelement mother liquor is strictly controlled to protect the activity of the thalli; and finally, based on the feedback of the survival rate of the finished product thalli, intelligently adjusting the technological parameters of the next batch. The prepared foliar bacterial fertilizer has the advantages of being high in thallus activity, comprehensive in function, good in storage stability and the like, and upgrading from a fixed formula to self-adaptive process optimization is achieved.
Owner:XINJIANG ZHONGNONG HONGYUAN AGRI TECH CO LTD +2
Application of Bacillus biopreparations in reducing the contamination of antibiotic resistance genes in vegetables
The present invention discloses the application of a Bacillus biological agent in reducing the contamination of antibiotic resistance genes in vegetables, belonging to the field of agricultural biotechnology. The present invention constructs a microbial agent containing one or more strains of Bacillus amyloliquefaciens B3, Bacillus subtilis B1, Bacillus atrophaeus B2, and Peribacillus frigoritolerans B4. By spraying the microbial agent constructed in the present invention on the leaf surface, the pressure of ARGs in hydroponic lettuce can be effectively alleviated, and the growth status and nutritional quality of hydroponic vegetables can be improved.
Owner:JIANGNAN UNIV
Microbial agent and application thereof in preventing and treating persimmon anthracnose
The present application belongs to the technical field of microorganisms, and particularly relates to a microbial agent and application thereof in prevention and treatment of persimmon anthracnose. The microbial agent comprises Bacillus amyloliquefaciens QGFCH3-3 and Bacillus atrophaeus HHQGTS13-1. The microbial agent can effectively inhibit the persimmon anthracnose pathogen and has a good prevention and treatment effect on the persimmon anthracnose.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP
A bacterial strain antagonistic to multiple pathogenic fungi of potato and its application
A bacterial strain antagonistic to multiple pathogenic fungi of potato and its application, which relate to the field of biological control, the strain is Bacillus atrophaeus Ba45, which is deposited in the General Microbiology Center of China Culture Collection Administration, with the deposit address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the deposit date: November 11, 2024, and the deposit number is CGMCC No. 32577. Bacillus atrophaeus Ba45 is used to prepare a bacterial agent antagonistic to potato pathogenic fungi. The Ba45 of the present invention greatly improves the comprehensive inhibition rate of Alternaria solani, Alternaria alternata, Fusarium solani, Fusarium oxysporum, Fusarium equisetum and grape stem blight. Therefore, Ba45 provides a more resistant high-quality strain for the comprehensive prevention and control of potato soil-borne diseases, and has good application prospects.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
A traditional Chinese medicine composite microbial fertilizer for inhibiting angelica root rot
The present invention relates to the technical field of fertilizer preparation, and in particular to a traditional Chinese medicine composite microbial fertilizer for inhibiting angelica root rot. The composite microbial fertilizer for inhibiting angelica root rot provided by the present invention comprises 45-55 parts of composite fertilizer, 20-26 parts of plant ash, 6-10 parts of humic acid, 3-7 parts of diatomaceous earth, 4-8 parts of a composite microbial agent, and 8-12 parts of a traditional Chinese medicine extract. The composite microbial agent comprises Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride, and Trichoderma harzianum, with a mass ratio of 14-18:12-16:11-15:8-12:8-12:5-9:5-9, each containing 400-600 million bacteria per gram. The traditional Chinese medicine extract comprises Scutellaria baicalensis, Ephedra sinica, Tagetes erecta, Nerium oleander, Ginkgo biloba, and Sophora flavescens. This fertilizer not only prevents and treats angelica root rot but also significantly improves the yield and quality of angelica, achieving excellent results.
Owner:INST OF SOIL FERTILIZER & WATER SAVING AGRI GANSU ACAD OF AGRI SCI
Bacillus atrophaeus and application thereof in salt tolerance and growth promotion of plants
The invention discloses bacillus atrophaeus and application thereof in salt tolerance and growth promotion of plants, and belongs to the technical field of microorganisms. The preservation number of the bacillus atrophaeus is CGMCC (China General Microbiological Culture Collection Center) Experimental results show that the bacillus atrophaeus provided by the invention has the salt-tolerant characteristic and the functions of fixing nitrogen, dissolving phosphorus, dissolving silicate, producing siderophores and the like, and can effectively promote absorption and utilization of plants to elements such as nitrogen, phosphorus, iron and the like so as to promote plant growth. The bacillus atrophaeus has an obvious improvement effect on the growth of wild barley plants under the stress of high-concentration salt and the endurance capacity of the wild barley plants, and can also remove heavy metals in high-salt wastewater. The invention provides a new growth-promoting bacterium for improving the high salt tolerance of plants, and provides a new technical means for high-concentration saline-alkali soil crop planting and wastewater heavy metal pollution treatment.
Owner:LANZHOU UNIV
Biocontrol bacteria and biocontrol bacterial agent for preventing and treating botrytis cinerea and application thereof
The present application relates to the field of microbial technology, a kind of biocontrol agent for preventing and treating tomato botrytis, biocontrol agent and its application.The biocontrol agent is Bacillus atrophaeus HLTL04 strain, preserved in China General Microbiological Culture Collection Center on June 20, 2023, and the preservation number is CGMCC NO.27658.The biocontrol agent of the present application has a strong inhibitory effect on tomato botrytis, and the HLTL04 strain can effectively inhibit the development of tomato rot caused by botrytis.The HLTL04 has a good growth-promoting effect on tomato plants.The HLTL04 strain can successfully colonize in tomato fruits.The Rif-resistant HLTL04 can colonize in tomato stems, leaves and roots, and shows a dynamic change trend of more at first and less later, gradually decreasing.The colonization amount in tomato rhizosphere soil gradually decreases, and the colonization amount in each part is different, with relatively large increase in roots compared to stems and leaves, indicating that HLTL04 can colonize and parasitize in tomato roots, stems and leaves for a long time, and the root surface of tomato plants is more suitable for its growth and reproduction than other parts.
Owner:CHENGDU UNIV
Microbial complex bacteria H1 for antagonizing multiple pathogenic fungi of potato and application and preparation method thereof
The application relates to a microbial compound bacterium H1 antagonizing multiple potato pathogenic fungi and an application and a preparation method thereof, and relates to the field of biological bacterial agents. The microbial compound bacterium H1 is mixed by bacillus subtilis, bacillus atrophaeus and bacillus cereus. The microbial compound bacterium H1 is used for preparing a bacterium agent antagonizing potato pathogenic fungi. The H1 has a strong inhibiting effect on the early blight pathogenic fungi alternaria solani and alternaria alternata, the dry rot pathogenic fungi fusarium solani, fusarium oxysporum and fusarium equiseti, and the leaf / stem spot pathogenic fungi botryodiplodia theobromae, and the inhibiting rates are all higher than 45%. The inhibiting rate on the botryodiplodia theobromae is the highest, reaching 76.92%. The H1 provides a high-quality strain for comprehensively preventing and treating potato soil-borne diseases, and has a good field application prospect.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Fermented poultry feed and preparation method thereof
The invention discloses a fermentation type poultry feed and a preparation method thereof, the fermentation type poultry feed comprises a fermentation inoculant and a fermentation substrate suitable for the fermentation type poultry feed, the fermentation inoculant suitable for the fermentation type poultry feed comprises bacillus atrophaeus (preservation number: CCTCC NO: M 2015765) and hansenula polymorpha (preservation number: CCTCC NO: M 20221245), and the fermentation substrate suitable for the fermentation type poultry feed comprises bacillus atrophaeus (preservation number: CCTCC NO: M 2015765) and hansenula polymorpha (preservation number: CCTCC NO: M 20221245). The fermentation substrate comprises fungus chaff powder, corncob powder, wheat bran powder, soybean meal powder, monopotassium phosphate, calcium carbonate and sugar, and the fungus chaff powder is prepared after black fungus is cultivated with the mulberry twig edible fungus cultivation material; when the feed prepared by the invention is used for feeding the broiler chickens, the feed-gain ratio in the feeding process can be effectively reduced, the feed utilization rate is increased, the immunity of the broiler chickens in the feeding process can be remarkably improved, and the meat quality of the broiler chickens is improved to a certain extent.
Owner:SICHUAN ACAD OF AGRI SCI SERICULTURE INST
Multi-strain microbial preparation for preventing and treating potato scab as well as preparation method and application of multi-strain microbial preparation
The invention relates to the technical field of microbial agents, and discloses a multi-strain microbial preparation for preventing and treating potato scab as well as a preparation method and application of the multi-strain microbial preparation. Wherein the multi-strain microbial preparation is prepared from bacillus atrophaeus 6052, bacillus compost 6069, microbacterium natalicum 6035 and bacillus atrophaeus 4618, and the bacillus atrophaeus 6052, the bacillus compost 6069, the microbacterium natalicum 6035 The bacillus atrophaeus 6052, the bacillus composting bacillus 6069, the microbacterium nataleckii 6035 and the bacillus atrophaeus 4618 are all preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the bacillus atrophaeus 6052, the bacillus composting bacillus 6069, the microbacterium nataleckii 6035 and the bacillus atrophaeus 4618 are respectively CGMCC NO.34693, CGMCC NO.34694, CGMCC NO.34695 and CGMCC The multi-strain microbial preparation disclosed by the invention is good and stable in antibacterial effect, and the morbidity and disease index of potato scab can be remarkably reduced after the multi-strain microbial preparation is used.
Owner:INNER MONGOLIA UNIVERSITY
A composite microbial fertilizer resistant to root rot
ActiveCN119661280BBio-organic fraction processingOrganic fertilisersBiotechnologyAngelica Sinensis Root
The present application relates to the technical field of fertilizer preparation, and particularly relates to a compound microbial fertilizer for preventing root rot. The compound microbial fertilizer comprises 45-55 parts of compound fertilizer, 20-26 parts of wood ash, 6-10 parts of humic acid, 3-7 parts of diatomite, 4-8 parts of compound microbial agent and 8-12 parts of traditional Chinese medicine extract. The compound microbial agent is composed of bacillus licheniformis, bacillus cereus, bacillus atrophaeus, pseudomonas chlororaphis, pseudomonas brassicacearum, trichoderma virens and trichoderma harzianum. The traditional Chinese medicine extract is composed of radix scutellariae, ephedra, tagetes, oleander, ginkgo leaves and sophora flavescens in a mass ratio of 10-16:13-17:7-11:8-12:4-8:3-7. The traditional Chinese medicine extract is obtained by mixing the above-mentioned traditional Chinese medicine materials, adding water and decocting, and then filtering. The compound microbial fertilizer can not only prevent and treat the root rot of angelica, but also significantly improve the yield and quality of angelica.
Owner:INST OF SOIL FERTILIZER & WATER SAVING AGRI GANSU ACAD OF AGRI SCI
Application of bacillus atrophaeus in fermentation production of gamma-polyglutamic acid
The invention belongs to the technical field of biology, and particularly discloses application of bacillus atrophaeus to fermentation production of polyglutamic acid, the bacillus atrophaeus is bacillus atrophaeus NWS-01 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus atrophaeus is CGMCC No. 29755. The invention further discloses a preparation method of the polyglutamic acid. In the invention, the main component of the extracellular polymeric substance produced by fermentation of the bacillus atrophaeus NWS-01 is reported and identified to be gamma-polyglutamic acid for the first time, the yield of the gamma-polyglutamic acid reaches 37.2 g / L through optimization of culture conditions and culture medium composition and is increased by 121.43% compared with that before optimization, the fermentation period is shortened by 8.33%, and the bacillus atrophaeus NWS-01 is suitable for industrial production and has a remarkable industrial application prospect.
Owner:XUANKAI BIOTECHNOLOGY (SHANDONG) CO LTD +1
Bacillus atrophaeus DG-19 and application of bacillus atrophaeus DG-19 in lily yellowing and / or successive cropping obstacles
The invention provides bacillus atrophaeus DG-19 and application of the bacillus atrophaeus DG-19 in lily yellowing and / or successive cropping obstacles, and belongs to the technical field of functional strains. The invention provides bacillus atrophaeus DG-19 and a microbial inoculum containing the bacillus atrophaeus DG-19, which are mainly used for improving chlorophyll content, jasmonic acid content and oxidation resistance, promoting cyclic utilization of nitrogen and phosphorus and absorption of potassium, degrading autotoxic substances, increasing abundance of probiotic bacteria and fungi and reducing abundance of pathogenic bacteria and fungi, so that the bacillus atrophaeus DG-19 and the microbial inoculum containing the bacillus atrophaeus DG-19 can be used for preparing the microbial inoculum. Therefore, physiological and biochemical states and rhizosphere microflora of the lily are effectively changed, lily yellowing is relieved, and continuous cropping obstacles of the lily are reduced.
Owner:GANSU ACAD OF SCI INST OF BIOLOGY +1
Cucumber root-knot nematode control method
The invention belongs to the technical field of pest control, and particularly discloses a cucumber root-knot nematode control method. The prevention and control method comprises the following steps: (1) field soil treatment: firstly scattering farmyard manure into soil, then scattering lime nitrogen, then deeply ploughing the soil, and then ridging and bedding; (2) transplanting and field planting: transplanting and field planting the cucumber seedlings into field soil; (3) management: applying a control composition on the day of cucumber field planting, and applying bacillus atrophaeus after 6-8 days; in the initial flowering stage of the cucumbers, gamma-aminobutyric acid is applied, and after 6-8 days, escort bacillus is applied; applying the prevention and treatment composition in the fruiting period of cucumbers; the prevention and treatment composition comprises gamma-aminobutyric acid and 5-aminolevulinic acid. According to the control method, specific preparations are applied in stages, so that root-knot nematodes can be effectively controlled, cucumber growth can be effectively promoted, and the yield is increased.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP +1
Microbial agent and application thereof in prevention and treatment of persimmon anthracnose
The invention belongs to the technical field of microorganisms, and particularly relates to a microbial agent and application thereof in prevention and treatment of persimmon anthracnose. The microbial agent disclosed by the invention is prepared from bacillus amyloliquefaciens QGFCH3-3 and bacillus atrophaeus HHQGTS13-1, and the microbial agent disclosed by the invention is prepared from bacillus amyloliquefaciens QGFCH3-3 and bacillus atrophaeus HHQGTS13-1, and the microbial agent disclosed by the invention is prepared from bacillus amyloliquefaciens QGFCH3-3 and bacillus atrophaeus HHQGTS13-1. The microbial agent can effectively inhibit persimmon anthracnose pathogenic bacteria, and has a good control effect on persimmon anthracnose.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP
Composite bacteria and application thereof in resource utilization of kitchen waste
The application relates to the technical field of kitchen garbage treatment, and particularly discloses a composite bacteria and application of the composite bacteria in kitchen garbage resource utilization. The composite bacteria disclosed by the application comprises A components and B components; the A components are composed of bacillus velezensis, bacillus amyloliquefaciens, pseudomonas, bacillus atrophaeus, bacillus laterosporus and trichoderma harzianum; and the B components are composed of bacillus paralicheniformis, bacillus licheniformis, bacillus calidus and bacillus stearothermophilus. The technical scheme of the composite bacteria provided by the application can effectively improve the degradation effect of kitchen garbage, realizes the purposes of kitchen garbage reduction, harmlessness, resource utilization and energy consumption reduction in the treatment process, and the kitchen garbage resource utilization treatment product has excellent application effects as a soil conditioner and feed.
Owner:BEIJING GOLDENWAY BIO TECH