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17 results about "Bacillus atrophaeus" patented technology
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Bacillus atrophaeus is a species of black-pigmented bacteria. Its type strain is NRRL NRS-213. B. atrophaeus strains have been used extensively in biomedicine as indicator strains for heat- and chemical-based decontamination regimens. Most of the strains in use are derivatives of a lineage of B. atrophaeus that originated at Camp Detrick in the 1950s, where many modern biocontainment procedures were developed. B. atrophaeus has historically been known by several other names, including B. globigii (the origin of its military moniker "BG") and B. subtilis var. niger. Modern phylogenetic analyses using multiple genetic methods have placed B. atrophaeus close to B. subtilis. Its original and still most prominent use is as a surrogate organism for pathogenic B. anthracis, beginning in the U.S. bio-weapons program, as its pigmentation readily facilitated discrimination from non-pigmented background organisms in environmental samples. Subsequent genomic and phenotypic analysis of strains derived from the Camp Detrick isolates revealed that they had been deliberately selected for strains that exhibited elevated rates of sporulation.
A method for promoting production of gamma-polyglutamic acid by bacillus fermentation using an oxygen carrier
This invention relates to the field of microbial fermentation, specifically to a method for promoting the fermentation of Bacillus atrophaeus to produce γ-polyglutamic acid (PGA) using oxygen carriers. This invention involves adding specific alkane oxygen carriers during the initial stage of γ-PGA production via Bacillus atrophaeus NWS-01 fermentation. Hexane, heptane, and hexadecane were selected as effective oxygen carriers, and the optimal single-addition concentration and optimal synergistic addition ratio of each oxygen carrier were determined. This solves the problem of limited γ-PGA yield due to low oxygen transfer efficiency in traditional fermentation processes. Specifically, adding 0.3% heptane alone achieves a γ-PGA yield of 39.4 ± 0.19 g / L, while the synergistic addition of 0.240% hexane, 0.407% heptane, and 1.014% hexadecane results in a maximum yield of 42.1 g / L, representing a 150.6% increase compared to the fermentation system without added oxygen carriers. This method is simple to operate, has high oxygen utilization, low energy consumption, and produces fewer bubbles and lower liquid shear force in the fermentation system, making it suitable for the industrial-scale aerobic high-density fermentation production of γ-PGA.
Owner:XUANKAI BIOTECHNOLOGY (SHANDONG) CO LTD
Sterilization biological indicator and application thereof
PendingCN121065021ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologySporeling
The invention relates to the technical field of biology, in particular to a sterilization biological indicator and application thereof. The invention provides a biological indicator composed of sulfhydrylated spores containing bacillus atrophaeus and a spore culture solution containing a gold nanoprobe. The biological indicator is used for monitoring the sterilization effect, solves the problems of low spore detection speed of bacillus atrophaeus after ethylene oxide sterilization and high use cost of a rapid ethylene oxide indicator, can realize rapid detection within 10 minutes after sterilization, and does not need a complex instrument. The method has a wide application prospect in the monitoring of the sterilization effect of ethylene oxide sterilization equipment.
Owner:SHINVA MEDICAL INSTR CO LTD
Biocontrol bacillus strain for relieving parasitic sunflower of orobanche coerulescens as well as derivative product and application of biocontrol bacillus strain
The invention provides a biocontrol bacillus strain for relieving parasitic sunflower of orobanche coerulescens as well as a derivative product and application of the biocontrol bacillus strain, and belongs to the technical field of biocontrol microorganisms. The invention firstly provides an application of bacillus atrophaeus or a biocontrol microbial inoculum thereof in prevention and control of Orobanche coerulescens parasitic sunflowers, and further provides an application of the Orobanche coerulescens in prevention and control of Orobanche coerulescens. Meanwhile, the invention also provides a method. The strain B45 is prepared into a biocontrol microbial inoculum, the biocontrol microbial inoculum has the advantages of being safe, economical and high in specialization, and the biocontrol microbial inoculum achieves the purpose of preventing and treating orobanche coerulescens by reducing the population density of pests and does not cause pollution to the environment.
Owner:CHINA AGRI UNIV
Bacillus atrophaeus SCAU-H1 capable of repairing heavy metal pollution and application thereof
The present application belongs to the technical field of pollution remediation, and particularly relates to Bacillus atrophaeus SCAU-H1 capable of remediation of heavy metal pollution and application thereof. The present application obtains a strain of Bacillus atrophaeus SCAU-H1, which is preserved in Guangdong Microbial Culture Collection Center on December 22, 2023, with a preservation number of GDMCC NO: 64181. The strain SCAU-H1 can effectively remove As(III), As(V), Cd, Pb and Sb heavy metals in water, and particularly has excellent removal effect on As(V). Moreover, the strain can significantly reduce the effective content of heavy metals Cd and Pb in soil, and has a significant immobilization effect. Therefore, the strain SCAU-H1 can be used for remediation and treatment of heavy metal contaminated water and soil.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
A bacterium-chemical composition for preventing and treating potato scab and application thereof
ActiveCN119867083BImprove the effect of prevention and controlreduce morbidityBiocideBacteriaBiotechnologyChemical control
This invention belongs to the field of potato scab control technology, specifically relating to a fungal-agent composition for controlling potato scab and its application. The fungal-agent composition for controlling potato scab provided by this invention comprises the following raw materials: a chemical control agent for potato scab and a compound fungal agent; the volume ratio of the chemical control agent to the compound fungal agent is 75-150:5000; the compound fungal agent includes *Bacillus belyceta* BEV2 and *Bacillus atrophus* FM2-4. Using the fungal-agent composition of this invention can reduce the dosage of chemical agents by at least half, while providing rapid, long-lasting, and effective control of potato scab, and also taking into account the advantages of environmental friendliness.
Owner:HEBEI AGRICULTURAL UNIV.
Synthetic bacterial flora co-culture fermentation broth, bacterial agent and application thereof
This invention relates to the field of agricultural biological control technology, specifically to a synthetic microbial co-culture fermentation broth, its inoculum, and its application. The synthetic microbial co-culture fermentation broth is prepared by sequential co-inoculation fermentation of *Trichoderma harzianum* TW21990, *Burkholderia viminalis* P418, and *Bacillus atrophicus* BsR05; wherein *Trichoderma harzianum* TW21990 has the CGMCC No. 12864, *Burkholderia viminalis* P418 has the CGMCC No. 1212, and *Bacillus atrophicus* BsR05 has the CGMCC No. 11665. Compared to single biocontrol strains, the antibacterial and growth-promoting secondary metabolites produced by the above-mentioned synthetic microbial co-culture fermentation are broad-spectrum, making it less likely to induce drug resistance in the control of gray mold in leeks. The control effect is stable, there is no antagonism within the synthetic microbial community, and the efficacy of the strains is synergistically enhanced, with a significant growth-promoting effect on leeks.
Owner:BIOTECH CENT OF SHANDONG ACAD OF SCI
Preparation method of high-activity multifunctional foliar bacterial fertilizer
The invention relates to the technical field of agricultural microbial fertilizers, in particular to a preparation method of a high-activity multifunctional foliar bacterial fertilizer, which comprises the following steps: preparing a composite culture medium, and accurately calculating the mass ratio of fish peptone to trehalose based on a target carbon nitrogen ratio; inoculating bacillus atrophaeus, bacillus siamensis and pseudomonas fluorescens in a specific proportion into the culture medium, and performing dynamic fed-batch fermentation; after fermentation, thalli are wrapped by forming protective colloid, and the addition condition of the microelement mother liquor is strictly controlled to protect the activity of the thalli; and finally, based on the feedback of the survival rate of the finished product thalli, intelligently adjusting the technological parameters of the next batch. The prepared foliar bacterial fertilizer has the advantages of being high in thallus activity, comprehensive in function, good in storage stability and the like, and upgrading from a fixed formula to self-adaptive process optimization is achieved.
Owner:XINJIANG ZHONGNONG HONGYUAN AGRI TECH CO LTD +2
Microbial agent and application thereof in preventing and treating persimmon anthracnose
The present application belongs to the technical field of microorganisms, and particularly relates to a microbial agent and application thereof in prevention and treatment of persimmon anthracnose. The microbial agent comprises Bacillus amyloliquefaciens QGFCH3-3 and Bacillus atrophaeus HHQGTS13-1. The microbial agent can effectively inhibit the persimmon anthracnose pathogen and has a good prevention and treatment effect on the persimmon anthracnose.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP
Bacillus atrophaeus and application thereof in salt tolerance and growth promotion of plants
The invention discloses bacillus atrophaeus and application thereof in salt tolerance and growth promotion of plants, and belongs to the technical field of microorganisms. The preservation number of the bacillus atrophaeus is CGMCC (China General Microbiological Culture Collection Center) Experimental results show that the bacillus atrophaeus provided by the invention has the salt-tolerant characteristic and the functions of fixing nitrogen, dissolving phosphorus, dissolving silicate, producing siderophores and the like, and can effectively promote absorption and utilization of plants to elements such as nitrogen, phosphorus, iron and the like so as to promote plant growth. The bacillus atrophaeus has an obvious improvement effect on the growth of wild barley plants under the stress of high-concentration salt and the endurance capacity of the wild barley plants, and can also remove heavy metals in high-salt wastewater. The invention provides a new growth-promoting bacterium for improving the high salt tolerance of plants, and provides a new technical means for high-concentration saline-alkali soil crop planting and wastewater heavy metal pollution treatment.
Owner:LANZHOU UNIV
Biocontrol bacteria and biocontrol bacterial agent for preventing and treating botrytis cinerea and application thereof
The present application relates to the field of microbial technology, a kind of biocontrol agent for preventing and treating tomato botrytis, biocontrol agent and its application.The biocontrol agent is Bacillus atrophaeus HLTL04 strain, preserved in China General Microbiological Culture Collection Center on June 20, 2023, and the preservation number is CGMCC NO.27658.The biocontrol agent of the present application has a strong inhibitory effect on tomato botrytis, and the HLTL04 strain can effectively inhibit the development of tomato rot caused by botrytis.The HLTL04 has a good growth-promoting effect on tomato plants.The HLTL04 strain can successfully colonize in tomato fruits.The Rif-resistant HLTL04 can colonize in tomato stems, leaves and roots, and shows a dynamic change trend of more at first and less later, gradually decreasing.The colonization amount in tomato rhizosphere soil gradually decreases, and the colonization amount in each part is different, with relatively large increase in roots compared to stems and leaves, indicating that HLTL04 can colonize and parasitize in tomato roots, stems and leaves for a long time, and the root surface of tomato plants is more suitable for its growth and reproduction than other parts.
Owner:CHENGDU UNIV
Microbial complex bacteria H1 for antagonizing multiple pathogenic fungi of potato and application and preparation method thereof
The application relates to a microbial compound bacterium H1 antagonizing multiple potato pathogenic fungi and an application and a preparation method thereof, and relates to the field of biological bacterial agents. The microbial compound bacterium H1 is mixed by bacillus subtilis, bacillus atrophaeus and bacillus cereus. The microbial compound bacterium H1 is used for preparing a bacterium agent antagonizing potato pathogenic fungi. The H1 has a strong inhibiting effect on the early blight pathogenic fungi alternaria solani and alternaria alternata, the dry rot pathogenic fungi fusarium solani, fusarium oxysporum and fusarium equiseti, and the leaf / stem spot pathogenic fungi botryodiplodia theobromae, and the inhibiting rates are all higher than 45%. The inhibiting rate on the botryodiplodia theobromae is the highest, reaching 76.92%. The H1 provides a high-quality strain for comprehensively preventing and treating potato soil-borne diseases, and has a good field application prospect.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Application of bacillus atrophaeus in fermentation production of gamma-polyglutamic acid
The invention belongs to the technical field of biology, and particularly discloses application of bacillus atrophaeus to fermentation production of polyglutamic acid, the bacillus atrophaeus is bacillus atrophaeus NWS-01 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus atrophaeus is CGMCC No. 29755. The invention further discloses a preparation method of the polyglutamic acid. In the invention, the main component of the extracellular polymeric substance produced by fermentation of the bacillus atrophaeus NWS-01 is reported and identified to be gamma-polyglutamic acid for the first time, the yield of the gamma-polyglutamic acid reaches 37.2 g / L through optimization of culture conditions and culture medium composition and is increased by 121.43% compared with that before optimization, the fermentation period is shortened by 8.33%, and the bacillus atrophaeus NWS-01 is suitable for industrial production and has a remarkable industrial application prospect.
Owner:XUANKAI BIOTECHNOLOGY (SHANDONG) CO LTD +1
Bacillus atrophaeus DG-19 and application of bacillus atrophaeus DG-19 in lily yellowing and / or successive cropping obstacles
The invention provides bacillus atrophaeus DG-19 and application of the bacillus atrophaeus DG-19 in lily yellowing and / or successive cropping obstacles, and belongs to the technical field of functional strains. The invention provides bacillus atrophaeus DG-19 and a microbial inoculum containing the bacillus atrophaeus DG-19, which are mainly used for improving chlorophyll content, jasmonic acid content and oxidation resistance, promoting cyclic utilization of nitrogen and phosphorus and absorption of potassium, degrading autotoxic substances, increasing abundance of probiotic bacteria and fungi and reducing abundance of pathogenic bacteria and fungi, so that the bacillus atrophaeus DG-19 and the microbial inoculum containing the bacillus atrophaeus DG-19 can be used for preparing the microbial inoculum. Therefore, physiological and biochemical states and rhizosphere microflora of the lily are effectively changed, lily yellowing is relieved, and continuous cropping obstacles of the lily are reduced.
Owner:GANSU ACAD OF SCI INST OF BIOLOGY +1
Cucumber root-knot nematode control method
The invention belongs to the technical field of pest control, and particularly discloses a cucumber root-knot nematode control method. The prevention and control method comprises the following steps: (1) field soil treatment: firstly scattering farmyard manure into soil, then scattering lime nitrogen, then deeply ploughing the soil, and then ridging and bedding; (2) transplanting and field planting: transplanting and field planting the cucumber seedlings into field soil; (3) management: applying a control composition on the day of cucumber field planting, and applying bacillus atrophaeus after 6-8 days; in the initial flowering stage of the cucumbers, gamma-aminobutyric acid is applied, and after 6-8 days, escort bacillus is applied; applying the prevention and treatment composition in the fruiting period of cucumbers; the prevention and treatment composition comprises gamma-aminobutyric acid and 5-aminolevulinic acid. According to the control method, specific preparations are applied in stages, so that root-knot nematodes can be effectively controlled, cucumber growth can be effectively promoted, and the yield is increased.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP +1
Microbial agent and application thereof in prevention and treatment of persimmon anthracnose
The invention belongs to the technical field of microorganisms, and particularly relates to a microbial agent and application thereof in prevention and treatment of persimmon anthracnose. The microbial agent disclosed by the invention is prepared from bacillus amyloliquefaciens QGFCH3-3 and bacillus atrophaeus HHQGTS13-1, and the microbial agent disclosed by the invention is prepared from bacillus amyloliquefaciens QGFCH3-3 and bacillus atrophaeus HHQGTS13-1, and the microbial agent disclosed by the invention is prepared from bacillus amyloliquefaciens QGFCH3-3 and bacillus atrophaeus HHQGTS13-1. The microbial agent can effectively inhibit persimmon anthracnose pathogenic bacteria, and has a good control effect on persimmon anthracnose.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP
Compound microbial agent for preventing and treating ginger bacterial wilt of small yellow ginger
The invention discloses a compound microbial agent for preventing and treating ginger bacterial wilt of small yellow ginger, and belongs to the technical field of biology, the compound microbial agent is prepared from a bacillus velezensis microbial agent, a bacillus atrophaeus microbial agent and a trichoderma asperellum microbial agent according to the volume ratio of 1: (1-3): (2-5), and the compound microbial agent is prepared from a bacillus velezensis microbial agent, a bacillus atrophaeus microbial agent and a trichoderma asperellum microbial agent according to the volume ratio of the bacillus velezensis microbial agent to the bacillus atrophaeus microbial agent to the trichoderma asperellum microbial agent according to the volume ratio of 1: (1-3): (2-5). The effective viable count in the bacillus velezensis microbial inoculum, the bacillus atrophaeus microbial inoculum and the trichoderma asperellum microbial inoculum is not less than 1 * 10 < 8 > CFU / ml. The compound microbial agent provided by the invention can be used for effectively preventing and treating the ginger bacterial wilt caused by R.S (ralstonia solanacearum).
Owner:INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
Bacillus atrophaeus GTW-1 and application thereof
The invention belongs to the field of biocontrol microorganisms, and discloses bacillus atrophaeus GTW-1 and application thereof. The invention provides a heat-resistant strain GTW-1 which is separated from a healthy wheat plant in a wheat disease parcel in Hebei Handan Greenhouse, the heat-resistant strain GTW-1 is classified and named as bacillus atrophaeus, and the preservation number of the heat-resistant strain GTW-1 is CGMCC No.36676. The heat-resistant strain GTW-1 is named as bacillus atrophaeus. The strain GTW-1 disclosed by the invention is high in control effect, and the control effect on the wheat powdery mildew caused by the Blumeria brucellosis f.sp. Tritici of the gramineous Blumeria brucellosis is 92.89%; the compound also has a relatively high prevention effect of 92.21% on wheat scab caused by fusarium graminearum schw., and the compound also has a relatively high prevention effect of 92.21% on wheat scab caused by fusarium graminearum schw. The strain provided by the invention has the advantages of lasting drug effect, difficult generation of drug resistance and environmental friendliness, and has a good application prospect.
Owner:INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI