122 results about "Bacillus siamensis" patented technology

The invention discloses a Bacillus siamensis strain for preventing and treating pear ring rot and soft rot and application thereof. The Bacillus siamensis strain is separated from peels of Laiyang pears with sterilized surfaces, the strain has broad-spectrum resistance for various types of phytopathogens and has a good effect on preventing and treating the pear fruit rot caused by pear ring rot and soft rot. The strain comes from pear fruits and is safe to pear fruits; the strain is produced on a large scale, is low in raw material cost, convenient to operate and stable in prevention and treatment effect, and can effectively solve the problems of agricultural residues and environment pollutions caused by pear fruit disease and insect pesticide prevention and treatment. The strain has huge application potential on the aspects of biological prevention and treatment of after-harvest diseases and insects of pears, and the excellent basic strain is provided for research and development of microbial pesticides.

The invention provides bacillus siamensis and application of the bacillus siamensis in the preparation of a microbial seaweed fertilizer. The bacillus siamensis L13 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on 6th March 2013 under the CGMCC No: 7285. The screened high-activity bacillus siamensis fermentation liquor provided by the invention is used for preparing a disinfectant and a promoter, and also can be applied to preparing the efficient microbial seaweed fertilizer. The microbial seaweed fertilizer is not only complete in nutrition, but also prominent in stress resistance, disease and insect resistance, and growth promoting capacity, can replace a farm chemical, a fertilizer, and a hormone, improves soil properties and product quality and causes the product to reach the green or organic standard.

The invention relates to the field of biological prevention and treatment of plant diseases, in particular to a bacillus siamensis strain and application thereof. The strain is preserved in CGMCC (China General Microbiological Culture Collection Center); the preservation code is CGMCC NO.12903; the preservation time is August 24, 2016. The strain is used for preventing and treating rhizoctonia solani kuhn; the potential adverse influence of chemical drug used in the current stage on the human environment and the rice safety can be avoided. The strain has the characteristics that the growth speed is high; the sporulation quantity is large; the action spectrum is wide; the stress resistance is great; the plant growth is promoted, and the like. Therefore the strain belongs to a biological prevention and treatment preparation with great application prospects.

The invention discloses Siam bacillus and application thereof to prevention and control of fusarium graminearum. The collection number of the disclosed Siam bacillus is CGMCC No.9501. As a biological prevention and control material of wheat scab, the Siam bacillus has a very good application prospect on the aspect of development of novel bio-pesticides or biological prevention and control microbial agents.

The invention relates to a compound microbial agent for high-temperature compost organic fertilizer of animal manure and straw and a preparation method thereof. The compound microbial agent comprises,by weight, 20 to 30 part of Siamese bacillus preparation, 40 to 60 parts of licheniformis preparation and 10 to 20 part of aspergillus niger powder preparation, wherein that composite microbial agentcomprises 20 to 30 parts of Siamese bacillus preparation, 40 to 60 part of licheniformis preparation and 10 to 20 parts of aspergillus niger powder preparation. A composite microbial inoculum is obtained by uniformly mixing the microbial inoculum. The compound microbial agent prepared by the invention is suitable for innocuous treatment and resource utilization of waste resources produced in agricultural production, such as livestock manure and straw, for high-temperature compost organic fertilizer. The fermentation period is shortened and the treatment efficiency is improved; Kill pathogensand parasite eggs thoroughly, improve the maturity of fertilizer. Removal of fecal odor in the fermentation process to reduce the impact of fermentation on the surrounding environment.

The invention provides Bacillus siamensis and application thereof, and belongs to the technical field of microbial prevention and control. Research shows that Bacillus siamensis LZ88 provided by the invention has very good effects on the biological control of plant diseases such as tobacco brown spot, tobacco black shank, wheat scab, grape anthracnose, cucumber fusarium wilt, peanut fusarium and the like, the induction of the resistance to tobacco plant diseases and the like, and provides a new strain resource for biological prevention and control of main diseases of tobacco, such as brown spot, and the like, thereby having good practical application value.

The invention relates to a composite microbial agent used for degradation of garden waste. The composite microbial agent is composed of composite bacterial liquid and a carrier, wherein the composite bacterial liquid comprises Bacillus subtilis, Bacillus licheniformis, Bacillus siamensis, Bacillus tequilensis and Phanerochaete chrysosporium. The bacterial strains of the composite microbial agent produce synergism, so the composite microbial agent can substantially degrade a variety of macro-molecular components in the garden waste, improve the decomposition degree of the waste, enhance the nutrient structure and microfloras of compost and reduce heavy metal pollution. The invention also relates to a preparation method for the composite microbial agent, a method for degrading and converting the garden waste by using the composite microbial agent and fertilizer prepared from products obtained after degradation and conversion of the garden waste with the composite microbial agent.

The invention discloses a bacillus siamensis (bacillus siamensis), and the preservation number of the bacillus siamensis is CGMCC No.19505. The bacillus siamensis has a very high inhibition effect on root rot caused by fusarium solani, the bacterial liquid of the bacillus siamensis has a relatively good prevention and treatment effect on the root rot of oil peonies, the growth of oil peony plants can be promoted, so that the investment of chemical fertilizers and pesticides can be reduced, the environmental pollution can be alleviated, and the sustainable development of agriculture can be realized.

The invention relates to a Bacillus siamensis YJ15 and an application thereof in biological control of blueberry gray mold. Bacillus siamensis strain YJ15 of that invention belong to the genus Bacillus sp., Deposited in the Common Microorganism Center of China Microorganism Culture Collection Management Committee (CGMCC), strain collection No. CGMCC No. 15952. Bacillus siamensis YJ15 is inoculatedin LB liquid culture medium to obtain fermentation seed liquid, and then the fermentation seed liquid is inoculated in fermentation culture liquid to obtain fermentation broth or supernatant of fermentation broth to obtain biological microbial agent. The biological bacterial agent prepared by the invention can control the blueberry gray mold and has good control effect equivalent to carbendazim.

The invention discloses a method for increasing content of ethyl caproate in Luzhou-flavor liquor. The method comprises the following steps: preparing a bacterial solution A, preparing a bacterial solution B, and preparing a composite functional bacterial solution C; preparing reinforced Daqu D; and preparation of pit mud microorganism enrichment liquid E and fermentation in a pool. According to the method, bacillus siamensis and candida utilis are selected for culture and fermentation, reinforced yeast is prepared, and the content of an ethyl caproate precursor substrate in the pit is increased through the synergistic effect of the reinforced yeast and the pit mud microorganism enrichment liquid, so that synthesis of ethyl caproate is promoted. When the method is used for brewing the Luzhou-flavor liquor, the ethyl caproate content of the raw liquor produced by the new cellar and the aging cellar is obviously improved, the Luzhou-flavor style of the liquor body is prominent, and the aroma is mellow.

The invention provides a salt-tolerant biocontrol bacterium B268 for the bacterial wilt of horsetail beefwood. The salt-tolerant biocontrol bacterium B268 for the bacterial wilt of horsetail beefwood is classified and named as Bacillus siamensis, and is preserved in China General Microbiological Collection Center, the preservation number is CGMCC No.13225, and the preservation date is November, 1, 2016. The strain is separated from seawater immersed root soil of horsetail beefwood, has an antagonistic activity for pathogenic bacterial strains of the bacterial wilt of horsetail beefwood and tomatoes, and also has the an activity of dissolving phosphorus, an activity of synthesizing a siderophore and a plant growth hormone and other activities of promoting plant growth. In addition, the tolerance scope of sea salt of the strain is wide, a stable biological film can be formed at high salt content, and the capabilities of growth and colonization are high, and therefore, the strain B268 can be applied to the biological prevention and control of the bacterial wilt of horsetail beefwood, is particularly applied to the preparation of a biological control agent or a pesticide for the bacterial wilt of horsetail beefwood, and is also applied to the preparation of a bacterial manure for promoting the growth and improving the disease resistance of horsetail beefwood.

The invention provides bacillus siamensis GL-02, the preservation number of which is CGMCC No.16068. A single colony formed by the strain propagating and growing on a beef extract peptone (NB) culturemedium is round, milky white, slightly bulgy in center, and moist and transparent on surface; through microscopic examination, the single colony is rod-shaped, flagellated, 0.7-0.8*1.6-2.2[mu]m in size, and G+,with spores. The single colony can grow on a beef extract peptone culture medium containing 2-5% NaCl. The formula of the beef extract peptone (NB) culture medium comprises 3g of beef extract, 10g of peptone, 12g of agar, and 1L of water, and the pH value is 6.8-7.0. The bacillus siamensis GL-02 has a significant antagonistic effect on pathogens causing corn stalk rot, bipolaris maydisand curvularia leaf spot. The invention also provides application of bacillus siamensis GL-02 in controlling plant fungal diseases, or application in preparing microbial preparations for controlling plant fungal diseases.

The invention discloses bacillus siamensis and application thereof in acetoin-rich table vinegar, bacillus siamensis QH-20009 can grow and metabolize under an acidic condition and produce amylase, granular amylase, pullulanase and protease at the same time, is applied to the field of food, and can remarkably improve the starch utilization rate and the nonvolatile acid content of a table vinegar brewing raw material, so that the yield of the vinegar is increased, and the production cost is reduced. The problem that the raw material utilization rate is not high in the vinegar brewing process can be solved, full utilization of grains is guaranteed, the amino acid content in the vinegar is increased, carbohydrates can be metabolized to produce acetoin, the acetoin content in the brewed vinegar can be increased, the flavor of the brewed vinegar can be improved, and the vinegar product quality can be improved.

The invention discloses a Siamese bacillus and an application of the same in controlling tomato root-knot nematode disease. The strain is identified as Bacillus siamensis LK0006 (CGMCC No. 15215). Theresults showed that the inhibition rate of nematodes is 90.35% when the fermentation broth is diluted 25 times and treated for 48 hours in vitro. The hatching of eggs could be inhibited by 25, 50, 100 times dilution of fermentation broth. Bacillus siamensis LK0006 wettable powder had a significant control effect on tomato root-knot nematode disease (75.02%) under pot culture condition, and tomatohad strong growth and no pesticide harm, so it had a good prospect in biocontrol of tomato root-knot nematode disease.

The invention discloses a microbial preparation for degrading aflatoxin and application, and belongs to the technical field of aflatoxin detoxification. An active ingredient of the microbial preparation for degrading the aflatoxin, which is disclosed by the invention, is a bacillus siamensis bacterium suspension or a culture of a bacillus siamensis whole culture solution or a crude extract of bacillus siamensis or an extracellular metabolite of the bacillus siamensis; and the bacillus siamensis is bacillus siamensis A2025 and is preserved in the China General Microbiological Culture CollectionCenter on April 12, 2018, the preservation number is CGMCC NO:15611, the address is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, and the preservation requiring institution is Shandong Peanut Research Institute. The bacillus siamensis A2025 disclosed by the invention has a high-efficiency degrading effect on the aflatoxin.

The invention relates to a bacterium. The bacterium is extracted from soil surrounding rhizospheres of diseased or infected plants and healthy plants in plot suffering from serious peanut soil-borne diseases, is biochemically identified as Bacillus siamensis ZHX-10, and is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.15609. The abovestrain can secrete antibacterial substances, so the bacterium can be used to prevent and control root rot and southern blight diseases, especially peanut sclerotium blight, Neocosmospora vasinfecta,Fusarium verticillioides, Fusarium oxysporum, Fusarium solani, Fusarium graminearum, Fusarium oxysporum Schlecht., Fusarium proliferatum and Gibberella fujikuroi.

The invention discloses a method for ensiling enteromorpha and a method for preparing enteromorpha crude polysaccharide. According to the method for ensiling the enteromorpha and the method for preparing the enteromorpha crude polysaccharide, a compound microbial agent which is a mixture of lactobacillus plantarum, lactobacillus brevis, bacillus siamensis and bacillus psychrosaccharolyticus is used for carrying out enteromorpha ensiling processing; after the enteromorpha is processed through the method for ensiling the enteromorpha, a solid cellulose structure can be easily resolved to be utilized; due to the fact that the acid hydrolysis technology is adopted, consumption of acid can be reduced, the degradation efficiency is also improved, the content of solid content in hydrolysate is increased, concentration does not need to be carried out, spray drying can be directly carried out, and production cost is reduced. The prepared enteromorpha crude polysaccharide can improve immunity and stress resistance capacity of crops. The method for ensiling the enteromorpha and the method for preparing the enteromorpha crude polysaccharide effectively solve the technical problems of enteromorpha ensiling processing, achieve long-term storage of the fresh enteromorpha, and are simple in operation, small in number of technological processes, low in production cost and applicable to industrialized mass production.

The invention discloses bacillus siamensis SW-2 with the collection number of GDMCC No.60287 as well as a method for preparing a microbial agent from the strain. The method comprises the steps as follows: after bacillus siamensis SW-2 is subjected to activation culture with an LB liquid medium, a fermentation tank is inoculated with the cultured bacillus siamensis SW-2 for enlargement culture withthe LB liquid medium; a fermentation tank is inoculated with a fermentation liquid obtained after enlargement culture for fermentation culture; a fermentation liquid obtained after fermentation culture is added to an auxiliary solid material, and drying, crushing and packaging are performed. The microbial agent can remarkably promote the temperature rise speed in the early period of composting, increase the fermentation temperature and prolong the high-temperature duration. After composting, the content of organic matter is increased, the content of C/N and ammonia nitrogen is reduced, the moisture content is reduced, the composting degree is high, the effects are significant, and mass application can be realized.

The invention discloses an application of bacillus siamensis B11 in prevention and/or treatment ofFargesia fungosa with sphaeropsis blight. According to the invention, the bacillus siamensis B11 is used for preventing and/or treating the Fargesia fungosa with sphaeropsis blight, the bacillus siamensis B11 is very good in effect, is long in storage time and high in strain survival rate after beingprepared into the nano microemulsion, has a good prevention effect on the Fargesia fungosa with sphaeropsis blight, has good stress resistance, high temperature resistance, low temperature resistanceand drying resistance, is suitable for commercialized production, is pollution-free, green and environment-friendly, can continuously control diseases and is low in production cost.

The invention provides Bacillus for antagonizing blight and application thereof, wherein the strain has a scientific name of Bacillus siamensis FJAT-47582. The Bacillus siamensis FJAT-47582 is preserved in the China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC NO. 15947. The Bacillus of the invention can not only inhibit fusarium oxysporum, xanthomonas axonopodis and other plant pathogenic bacteria, but also improve the vitality of plant seeds. The bacillus can be used for preventing and treating blight, and is a biocontrol bacterium being environment-friendly, economic and effective in terms of disease prevention and control.

The invention discloses bacillus siamensis capable of being used for repairing concrete cracks, a microbial inoculum and application. The bacillus siamensis is named as bacillus siamensis, the strainnumber of the bacillus siamensis is ZJB20040, and the preservation number of the bacillus siamensis is CCTCC NO: M 2020276. The bacillus siamensis ZJB20040 provided by the invention has a preservationnumber of CCTCC NO: M2020276, has very good thermal stability and acid-base stability, can adapt to environments with different pH values when being used for repairing concrete cracks, and has good potential and application prospect when being used for preparing a biological repairing preparation for self-repairing the concrete cracks.

The invention discloses a method for transforming Bacillus siamensis by electric shock, belonging to the technical field of microbial genetic engineering. The invention comprehensively optimizes factors such as preparation of competent cell, concentration of plasmid, electric shock parameter and the like during electric shock transformation of Bacillus siamensis JFL15, and establishes a simple andeffective genetic transformation method of Bacillus siamensis for the first time. The genetic transformation method of Bacillus siamensis of the invention is simple in operation, high in conversion rate, stable in effect, and can be used for the knockout of CRISPR -cas9 gene and the large plasmid () 11kbp for homologous recombination knockout vector with high efficiency, not only laid a good foundation for genetic modification of Bacillus siamensis, and has an important significance for accelerating its application in industrial production, but also provided an important reference for genetictransformation of other Bacillus spp.