22 results about "Bacillus siamensis" patented technology

The invention provides a tobacco and sweet potato relay intercropping field planting method and application thereof, and belongs to the technical field of agricultural planting. The planting method provided by the invention comprises the following steps: after tobacco is harvested, intensively burning diseased roots, diseased plants and weeds in a field, deeply ploughing soil in winter, carrying out soil drying and sterilization, applying an organic fertilizer or a special fertilizer for sweet potatoes by adopting a strip application mode, carrying out tobacco and sweet potato intercropping by adopting a single-side opposite planting mode, applying a bacillus siamensis XZ92 bacterial liquid and a bacillus velezensis XZ93 bacterial liquid to rhizosphere of the intercropped tobacco, and carrying out intercropping on the tobacco and the sweet potatoes by adopting a single-side opposite planting mode. And pouring methyl jasmonate liquid into the tobaccos, and supplementing trace elements in stages to finish planting. The comprehensive prevention and control system for the tobacco bacterial wilt is constructed by optimizing the relay intercropping space layout of tobacco and sweet potatoes, cooperatively applying strains with specific functions and methyl jasmonate and designing a staged nutrient supply scheme.

This invention relates to the field of microbial technology. This invention provides a strain of Bacillus sicca (…). Bacillus siamensis This invention provides strain F019, which was deposited on July 8, 2025, at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 35129. The invention also provides a microbial inoculant. Furthermore, the invention provides the application of Bacillus sicca strain F019 or the microbial inoculant. The Bacillus sicca strain F019 provided by this invention exhibits highly effective antibacterial activity against various plant diseases, including black shank, and significantly promotes plant disease resistance, plant growth, and seed germination. Strain F019 and AM fungi mutually promote each other's colonization in the tobacco rhizosphere; the combined application of strain F019 and AM fungi can significantly promote tobacco growth and enhance tobacco resistance to black shank.

The invention discloses a compound microbial fertilizer suitable for flue-cured tobacco production as well as a preparation method and application thereof. The compound microbial fertilizer is prepared from the following components in percentage by mass: 4-8% of a functional microbial agent, 12-18% of a first modifier, 1-2% of a second modifier, 30-70% of an inorganic material and the balance of pure water, wherein the first modifier is a polyglutamic acid stock solution in which the mass percent of polyglutamic acid is greater than or equal to 3.5%, and the second modifier is a gamma-aminobutyric acid stock solution in which the mass percent of gamma-aminobutyric acid is greater than or equal to 50%; the functional microbial agent is prepared by compounding a bacterial strain XNSD01 and a bacterial strain XNSD02, the bacterial strain XNSD01 is bacillus siamensis with the preservation number of CCTCC (China Center for Type Culture Collection) NO.M 20252934, and the bacterial strain XNSD02 is bacillus mycoides with the preservation number of CCTCC NO.M 20252935. And unification of disease resistance, growth promotion and successive cropping obstacle alleviation is realized.

The invention discloses bacillus siamensis and application thereof, and relates to the technical field of microorganisms. The strain is bacillus siamensis LYQ15 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO: 30024. The strain provided by the invention can be used for effectively inhibiting the growth and reproduction of phytophthora and pseudomonas solanacearum as well as related diseases caused by the growth and reproduction of the phytophthora and the pseudomonas solanacearum; in addition, the bacillus siamensis LYQ15 can inhibit reproduction of pathogenic bacteria and epidemic of diseases in an environment with the pathogenic bacteria, and healthy growth of tobacco plants is guaranteed.

The invention relates to the technical field of agricultural microbial fertilizers, in particular to a preparation method of a high-activity multifunctional foliar bacterial fertilizer, which comprises the following steps: preparing a composite culture medium, and accurately calculating the mass ratio of fish peptone to trehalose based on a target carbon nitrogen ratio; inoculating bacillus atrophaeus, bacillus siamensis and pseudomonas fluorescens in a specific proportion into the culture medium, and performing dynamic fed-batch fermentation; after fermentation, thalli are wrapped by forming protective colloid, and the addition condition of the microelement mother liquor is strictly controlled to protect the activity of the thalli; and finally, based on the feedback of the survival rate of the finished product thalli, intelligently adjusting the technological parameters of the next batch. The prepared foliar bacterial fertilizer has the advantages of being high in thallus activity, comprehensive in function, good in storage stability and the like, and upgrading from a fixed formula to self-adaptive process optimization is achieved.

The invention provides a strain of bacillus siamensis Bsiamensis and application thereof, the strain is classified and named as bacillus siamensis Bsiamensis, the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 35785, and the preservation date is September 1, 2025. The bacterial suspension, the fermentation liquor and the ethyl acetate extract of the fermentation liquor of the bacterial strain have good bacteriostatic activity on fusarium graminearum, fusarium oxysporum, fusarium laminatum, alternaria longibrachiata, colletotrichum gloeosporioides and colletotrichum frutescens.

The invention discloses a compound microorganism for preventing and treating chestnut fruit rot and application of the compound microorganism. The compound microorganism is composed of trichoderma atroviride, bacillus siamensis and bacillus velezensis, and is constructed by directionally screening strains with broad-spectrum inhibition effect on seven core pathogenic bacteria from Chinese chestnut fruit endophytes and verifying compatibility. The method has the advantages that the compound flora synergistically prevents and controls diseases through direct antagonism and induction of plant system resistance, and the defensive enzyme activity and the content of total polyphenol and total flavone of Chinese chestnuts are remarkably improved; according to the compound microorganism, the indoor control effect reaches 90% (the morbidity is reduced), the diseased fruit rates of Chinese chestnut fruits treated in a chestnut garden are reduced by 60% and 42.86% respectively after the Chinese chestnut fruits are stored for 30 days and 120 days, the effectiveness and the stability are obviously higher than those of a single strain, the compound microorganism can be introduced in the flowering period or sprayed in the storage period, use is convenient and flexible, and an efficient and stable biocontrol strategy is provided for green prevention and control of the Chinese chestnut fruit rot.

The invention discloses a bacillus siamensis fungicide, a biological selenium fertilizer and application of the bacillus siamensis fungicide and the biological selenium fertilizer in ecological restoration, and belongs to the technical field of soil salinization and heavy metal pollution prevention and control. A bacillus siamensis strain is screened from soil, and biological selenium particles which are uniform in particle size and rich in protein and polysaccharide functional groups on the surface are prepared in a culture system containing sodium selenite through biological reduction; then, the prepared biological selenium is independently applied or applied to plant rhizosphere or soil together with living bacillus siamensis to relieve salt-cadmium dual stress, and by adjusting the oxidation-reduction state and rhizosphere micro-ecology in the plant, Cdtoxicity is relieved, and the growth and physiological activity of the plant under salt-cadmium composite stress are improved. The prepared biological selenium is low in toxicity, good in stability, remarkable in synergistic effect with microorganisms, suitable for green remediation and efficient planting of salinized and heavy metal polluted farmlands and good in application prospect.

The invention discloses an alfalfa stress-resistant high-yield breeding method based on a microbiome regulation and control technology, and belongs to the technical field of microbial breeding. According to the breeding method, compound fertilizer doped with a microbial agent is applied in a seedling stage, a branching stage and a squaring stage respectively, and the compound fertilizer comprises a substrate and the microbial agent, the microbial agent is prepared from sinorhizobium meliloti, bacillus stearothermophilus, peste des petits ruminants, bacillus licheniformis, azotobacter brown, bacillus siamensis and bacillus amyloliquefaciens; the substrate comprises at least two of cow dung, pig manure, bean bran, corn straw and wood chips. According to the breeding method, the new microbial composition is prepared for preparing the compound fertilizer, the nutrition absorption capacity of the alfalfa can be improved through a microbial regulation function, and then the stress resistance and yield of the alfalfa are improved.

The application discloses a bacillus siamensis strain and application of the bacillus siamensis strain in fermentation of rapeseed meal. Bacillus siamensis The application provides the bacillus siamensis (BSJ-2) strain with a preservation number of CGMCC No.32638. The strain or culture solution or bacteria liquid or fermentation liquor thereof is applied to any one of the following or preparation of any one of the following products: 1) degradation of rapeseed meal storage protein; 2) improvement of rapeseed meal nutritional quality; 3) improvement of rapeseed meal protein utilization rate; the application finds that the bacillus siamensis (BSJ-2) strain can degrade rapeseed meal storage protein, improve rapeseed meal nutritional quality and improve rapeseed meal protein utilization rate. Bacillus siamensis The fermentation product of the bacillus siamensis (BSJ-2) strain can be used for preparing animal feed.

TITLE: USE OF A BACILLUS SIAMENSIS STRAIN AS A BIOSOLUTION IN TOMATO CULTIVATION The present invention relates to the use of a particular bacterial species as a biosolution in tomato production and cultivation. More specifically, the invention relates to the use of a Bacillus siamensis strain, deposited with the National Collection of Microorganism Cultures (CNCM) under the order number CNCM I-5921, in stimulating the development and growth of tomato seeds and seedlings or improving tomato crop yields, and in improving the resistance and tolerance to abiotic stress of seeds, seedlings, and tomato crops. [Fig. 1]

The invention relates to bacillus siamensis and an application of the bacillus siamensis in aquaculture. The bacillus siamensis is obtained by carrying out antagonistic strain separation and screening by taking vibrio parahaemolyticus as an indicator bacterium. Through verification, the strain has a relatively obvious inhibition effect on vibrio parahaemolyticus causing vibriosis in aquaculture, also has certain resistance to pathogenic bacteria such as vibrio alginolyticus and aeromonas hydrophila, in addition, the strain has relatively good high-temperature resistance, acid and alkali resistance and salt tolerance, and when the strain or a microbial agent prepared from the strain is applied to aquaculture, the yield of aquaculture is greatly increased. The compound has high inhibition rate on various germs including vibrio parahaemolyticus in a water body, does not influence the water body environment, has potential effects of promoting the growth of bred animals, stimulating the response of an immune system, improving the immunity, improving the water quality condition, repairing the ecological environment and the like, and has a wide application prospect.

The application discloses a kind of for preventing and treating chestnut fruit rot disease complex microorganism and its application.The application complex microorganism is composed of Trichoderma atroviride and Bacillus siamensis, Bacillus velezensis, by from chestnut fruit endophyte directional screening to seven kinds of core pathogenic bacteria have broad-spectrum inhibitory effect strain, is constructed after compatibility verification.It is superior in that: complex microbial population is synergistically controlled disease by direct antagonism and induced plant system resistance, significantly improves chestnut defense enzyme activity and total polyphenol, total flavone content;Indoor control efficiency reaches 90% (reduces the incidence), chestnut fruit in chestnut orchard treatment is reduced 60% and 42.86% respectively in 30 days and 120 days, effective and stability are obviously stronger than single strain, and the application complex microorganism can be introduced by flowering period or sprayed in storage period, and is convenient and flexible, provides efficient, stable biocontrol strategy for chestnut fruit rot green control.

The present application relates to the technical field of biocontrol bacteria, and particularly relates to a bacillus siamensis (Bacillus siamensis) ARF-SR1, a biocontrol bacterium and application thereof. Bacillus siamensis The present application provides the preservation number of the bacillus siamensis ARF-SR1 as CGMCC No.33715. The bacillus siamensis ARF-SR1 provided by the present application is a biocontrol strain isolated from a healthy alfalfa field in Ningxia, has a significant inhibitory effect on alfalfa root rot pathogenic bacteria (especially Fusarium solani, Fusarium oxysporum and Phoma sp.), an antagonistic rate of 57.71-68.86%, can reduce the incidence of alfalfa root rot, enriches the biocontrol bacteria resource library, and is suitable for green control of alfalfa root rot.

The invention discloses a compound microbial fertilizer and a preparation method thereof, and the compound microbial fertilizer is prepared from the following raw materials in parts by weight: 100-150 parts of a bacillus siamensis microbial agent, 150-200 parts of a streptomyces pourpuensis microbial agent, 130-175 parts of a synergist, 185-215 parts of an auxiliary material, 110-140 parts of a chelate material, 41-78 parts of a catalyst, 10-30 parts of an active agent, 1-3 parts of an inhibitor, 0.5-1.5 parts of a thickening agent and 100-150 parts of water. The preparation method of the compound microbial fertilizer is simple and easy to implement, all the materials are added according to a specific sequence, it can be guaranteed that the materials are fully dissolved and mixed, the stability and uniformity of the fertilizer are guaranteed, and industrial production is facilitated; by applying the compound microbial fertilizer disclosed by the invention, the problem of lack of trace elements of crops can be directly solved, the growth and development of the crops are comprehensively optimized through the synergistic interaction of amino acids, the goals of quality improvement, yield increase, disease resistance and environmental protection are achieved, and the compound microbial fertilizer conforms to the development requirements of green, high-end and efficient agriculture and has wide popularization and application prospects.

This invention discloses a strain of *Corynebacterium lucida* CYPJ1 and its applications. The *Corynebacterium lucida* CYPJ1 has the preservation number CGMCC No. 35719, and its saccharifying enzyme activity is 73.19 ± 0.09 U / mL. Using homogenized raw materials and fermentation with this strain results in increased soluble dietary fiber content and enhanced physical and functional properties. Foods or health products fermented with homogenized raw materials and this strain have lipid-lowering effects. This invention also found that using *Corynebacterium lucida* (…) after homogenization of raw materials… Clavispora lusitaniae CYPJ1 and Kluyveromyces macrocephala ( Kluyveromyces marxianus SXJ2 or Bacillus sicca ( Bacillus siamensis The DL-RXL01 combination fermentation yields even better results.

The application discloses a kind of rotten agent with disease prevention and control function and preparation method and application, belong to the cross field of agricultural microorganism technology and biological control.It is composed of compound bacteria powder and microcapsule fungicide by the rotten agent, the compound bacteria powder is made of bacillus subtilis ZKY01 bacteria powder and bacillus velezensis ZKY02 bacteria powder, and the microcapsule fungicide is bacillus siamensis ZKY03 bacteria powder embedded in microcapsule carrier;Wherein, the bacteria powder of three kinds of bacteria is the effective viable count not less than 1×10 11 CFU / g powder obtained by fermentation culture.The application can shorten the rotting period of agricultural organic waste such as straw, livestock and poultry manure to 12~15 days through the synergistic effect of three strains, and effectively prevent and control tobacco root black rot, black foot disease and other soil-borne diseases through the secretion of antibacterial substances, competition for nutrients and other mechanisms.

This invention provides a Bacillus sicca Z4, Bacillus siamensis The strain Z4 was deposited on March 3, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37830, located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The optimal fermentation medium for strain Z4 was 1000 mL of water, with yeast extract at 15 g / L, maltose at 20 g / L, and sodium chloride at 4 g / L. The optimal fermentation conditions were: 1% inoculum in a 250 mL Erlenmeyer flask (75 mL), temperature 35℃, pH 7, rotation speed 289 r / min, and incubation time 18 h. Strain Z4 antagonized the growth and spore germination of *Spodoptera litura*, the causal agent of sorghum smut. The aseptic fermentation filtrate of strain Z4, diluted 10-20 times, could be used to accelerate sorghum seed germination and emergence. The fermentation broth of strain Z4, diluted 10-100 times, could be used for the control of sorghum smut. This invention provides new biocontrol resources for the prevention and control of sorghum smut and other diseases.

The application belongs to the field of biological environmental protection technology, and particularly relates to a kind of antibiotic resistance reduction bacillus siamensis and microbial agent and application thereof.The preservation number of the antibiotic resistance reduction bacillus siamensis is CCTCC NO: M 20232193.The bacillus siamensis provided by the application can effectively reduce the antibiotic resistance of soil, can significantly reduce the abundance of antibiotic resistance bacteria and antibiotic resistance genes in soil, the average reduction rate of different antibiotic resistance bacteria and antibiotic resistance genes reaches 38.92% and 46.32%, can greatly reduce the proliferation and diffusion of ARGs and ARB in soil, significantly reduces the enrichment level, provides a new solution for efficient, pollution-free elimination of soil antibiotic resistance, has the potential to be applied to promote sustainable agriculture, animal husbandry development and improve soil health;In addition, the strain has strong biofilm formation ability, and is expected to be used as a biocontrol agent.

The invention provides an antagonistic bacterium P3 strain for brown spot of oyster mushroom, the classification name of the antagonistic bacterium P3 strain is bacillus siamensis, the antagonistic bacterium P3 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.34763. The antagonistic bacterium P3 strain has the advantages that the antagonistic bacterium P3 strain can be used for resisting brown spot of oyster mushroom; the P3 strain is found to have a good antagonistic effect on the brown spot of the oyster mushrooms for the first time, so that a solid foundation is laid for screening of biocontrol strains of the oyster mushrooms and biological prevention and control of diseases, and the P3 strain plays a role in promoting development of safe and efficient biopesticide preparations and has important theoretical significance and practical value. The result shows that the treatment effect of the P3 strain is 24.12%, and the prevention effect is 56.57%. In conclusion, the P3 strain has the biocontrol potential of preventing and treating the brown spot of the oyster mushrooms.

The present application relates to the technical field of biological control, and discloses bacillus siamensis and application thereof.The bacillus siamensis of the present application is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.32576.The bacillus siamensis has good prevention effect on pepper blight, and the field prevention effect can reach 71.69+ / -8.00%, and can be used for preventing and treating various plant diseases.

This invention relates to a strain of *Bacillus sicca* capable of producing volatile broad-spectrum antibacterial substances and its applications, belonging to the field of biological control technology. To address the shortcomings of chemical control of tomato gray mold, this invention provides a strain of *Bacillus sicca* and its applications. This strain, isolated from honeysuckle, produces IAA (inhibitory acetic acid), antimicrobial peptides, and volatile organic compounds. The volatile organic compounds produced by this strain exhibit broad-spectrum antibacterial activity, achieving a 100% inhibition rate against *Botrytis cinerea*, *Fusarium oxysporum*, *Fusarium graminearum*, and *Cucumis mellitus* var. *cubiclesii*. Furthermore, this strain demonstrates excellent control efficacy against tomato gray mold caused by *Botrytis cinerea* and can be used for the control of gray mold in tomato plants and for fruit preservation. Compared to traditional control measures, the *Bacillus sicca* strain provided by this invention offers advantages such as high efficiency, broad-spectrum disease resistance, easy spread and transmission, wide coverage, and low likelihood of developing drug resistance, making it a promising candidate for controlling diseases in greenhouse fruits and vegetables.