43 results about "Bacillus siamensis" patented technology

The invention provides a preparation method of a compound microbial agent and a pasture fermented feed, and belongs to the technical field of feed fermentation. The complex microbial inoculant disclosed by the invention comprises lactobacillus acidophilus, bacillus siamensis, bacillus amyloliquefaciens and pichia kluyveri. The lactobacillus acidophilus can produce acid and resist acid, and has better antibacterial performance; the bacillus siamensis can degrade lignin and reduce the content of neutral detergent fibers and acid detergent fibers in the fermented feed; the bacillus amyloliquefaciens produces cellulase and amylase with higher activity; the pichia kluyveri can increase the content of crude protein in the fermented feed and improve the nutritional performance of the feed. The four strains have a synergistic effect and jointly ferment forage feed, infectious microbes can be effectively inhibited, the fermented forage feed has excellent color, smell and texture, the palatability of the feed is improved, the appetite of animals is stimulated, and a good growth promoting effect is achieved; the fermented forage feed disclosed by the invention is high in nutritional quality and relatively high in feed utilization rate.

The invention discloses a microbial bacterium inhibitor as well as a preparation method and application thereof, the microbial bacterium inhibitor is fermentation liquor obtained by activating and fermenting bacillus siamensis YWF-25-21, and the microbial bacterium inhibitor has a relatively good prevention and treatment effect on tobacco black shank, fusarium root rot and rhizoctonia solani. The bacillus siamensis microbial agent prepared by the invention is high in spore content, can reduce crop disease loss, can effectively promote crop plant growth and development, and can enhance crop stress resistance; the method can meet the requirement of batch industrial production, ensures that products are more stably supplied on the market, directly reduces the production cost, improves the economic benefits, and is suitable for popularization and application.

The invention provides a tobacco and sweet potato relay intercropping field planting method and application thereof, and belongs to the technical field of agricultural planting. The planting method provided by the invention comprises the following steps: after tobacco is harvested, intensively burning diseased roots, diseased plants and weeds in a field, deeply ploughing soil in winter, carrying out soil drying and sterilization, applying an organic fertilizer or a special fertilizer for sweet potatoes by adopting a strip application mode, carrying out tobacco and sweet potato intercropping by adopting a single-side opposite planting mode, applying a bacillus siamensis XZ92 bacterial liquid and a bacillus velezensis XZ93 bacterial liquid to rhizosphere of the intercropped tobacco, and carrying out intercropping on the tobacco and the sweet potatoes by adopting a single-side opposite planting mode. And pouring methyl jasmonate liquid into the tobaccos, and supplementing trace elements in stages to finish planting. The comprehensive prevention and control system for the tobacco bacterial wilt is constructed by optimizing the relay intercropping space layout of tobacco and sweet potatoes, cooperatively applying strains with specific functions and methyl jasmonate and designing a staged nutrient supply scheme.

This invention relates to the field of microbial technology. This invention provides a strain of Bacillus sicca (…). Bacillus siamensis This invention provides strain F019, which was deposited on July 8, 2025, at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 35129. The invention also provides a microbial inoculant. Furthermore, the invention provides the application of Bacillus sicca strain F019 or the microbial inoculant. The Bacillus sicca strain F019 provided by this invention exhibits highly effective antibacterial activity against various plant diseases, including black shank, and significantly promotes plant disease resistance, plant growth, and seed germination. Strain F019 and AM fungi mutually promote each other's colonization in the tobacco rhizosphere; the combined application of strain F019 and AM fungi can significantly promote tobacco growth and enhance tobacco resistance to black shank.

The invention discloses a compound microbial fertilizer suitable for flue-cured tobacco production as well as a preparation method and application thereof. The compound microbial fertilizer is prepared from the following components in percentage by mass: 4-8% of a functional microbial agent, 12-18% of a first modifier, 1-2% of a second modifier, 30-70% of an inorganic material and the balance of pure water, wherein the first modifier is a polyglutamic acid stock solution in which the mass percent of polyglutamic acid is greater than or equal to 3.5%, and the second modifier is a gamma-aminobutyric acid stock solution in which the mass percent of gamma-aminobutyric acid is greater than or equal to 50%; the functional microbial agent is prepared by compounding a bacterial strain XNSD01 and a bacterial strain XNSD02, the bacterial strain XNSD01 is bacillus siamensis with the preservation number of CCTCC (China Center for Type Culture Collection) NO.M 20252934, and the bacterial strain XNSD02 is bacillus mycoides with the preservation number of CCTCC NO.M 20252935. And unification of disease resistance, growth promotion and successive cropping obstacle alleviation is realized.

The invention discloses Corynespora aspera CYPJ1 and an application of the Corynespora aspera CYPJ1. The preservation number of the corynespora aspera CYPJ1 is CGMCC (China General Microbiological Culture Collection Center) No.35719, and the saccharifying enzyme activity of the corynespora aspera CYPJ1 is 73.19 + / Raw materials are homogenized, the content of soluble dietary fibers prepared through fermentation of the strain is increased, and physical characteristics and functional characteristics are enhanced. The raw materials are homogenized, and food or health care products fermented by the strain have the effect of reducing blood fat. The invention also finds that after the raw materials are homogenized, the combined fermentation of clavipora lusitaniae CYPJ1 and kluyveromyces marxianus SXJ2 or bacillus siamensis DL-RXL01 is adopted, so that the effect is better. The invention also finds that after the raw materials are homogenized, the fermentation is carried out by adopting the combination of the clavipora lusitaniae CYPJ1 and the kluyveromyces marxianus SXJ2 or the bacillus siamensis DL-RXL01.

TITLE: USE OF A BACILLUS SIAMENSIS STRAIN AS A BIOSOLUTION IN MUSHROOM CULTIVATION The present invention relates to the use of a particular bacterial species as a biosolution in the production and cultivation of mushrooms. More specifically, the invention relates to the use of a Bacillus siamensis strain, registered with the CNCM under CNCM order number I-5921, in stimulating the development and growth of a mycelium or fungus, improving the yields of edible mushroom crops, and controlling the main fungal pathogens of edible mushroom crops. [Fig. 1]

The invention provides a composite pesticide for resisting fungal diseases of silage corn and a preparation method of the composite pesticide, and belongs to the technical field of pesticides. The composite agent comprises composite microbial pellets and an auxiliary agent, the mass ratio of the composite microbial pellets to the auxiliary agent is (9-25): (1-7). The compound microorganism bacterium balls comprise bacillus siamensis and bacillus niamurensis; the auxiliaries comprise rutin, luteolin and saikoside. The anti-fungal disease composite agent provided by the invention has an excellent prevention and treatment effect on fungal diseases of silage corn, the onset time of the composite agent can be shortened, microbial inactivation is avoided, the shelf life is prolonged, and the prevention and treatment efficiency on field fungal diseases is improved.

The invention discloses bacillus siamensis and application thereof, and relates to the technical field of microorganisms. The strain is bacillus siamensis LYQ15 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO: 30024. The strain provided by the invention can be used for effectively inhibiting the growth and reproduction of phytophthora and pseudomonas solanacearum as well as related diseases caused by the growth and reproduction of the phytophthora and the pseudomonas solanacearum; in addition, the bacillus siamensis LYQ15 can inhibit reproduction of pathogenic bacteria and epidemic of diseases in an environment with the pathogenic bacteria, and healthy growth of tobacco plants is guaranteed.

The invention relates to bacillus siamensis and application thereof. The invention finds that the bacillus siamensis strain Bs-1117 has an antagonistic inhibition effect on fusarium solani, fusarium oxysporum, fusarium pseudograminearum, fusarium verticillium, fusarium graminearum or botrytis cinerea, so that the bacillus siamensis strain Bs-1117 can be used for preventing and treating root rot, fusarium wilt, stem rot, spike rot, gibberellic disease or gray mold.

The invention relates to the technical field of microorganisms. The invention provides a bacillus siamensis strain F019 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on July 8, 2025, and the preservation registration number of the bacillus siamensis strain F019 is CGMCC No.35129. The bacillus siamensis strain F019 has the advantages that the bacillus siamensis strain F019 can be used for preparing the bacillus siamensis strain F019; the invention also provides a microbial agent. The invention further provides application of the bacillus siamensis strain F019 or a microbial agent. The bacillus siamensis F019 provided by the invention has an efficient bacteriostatic effect on various plant diseases including black shank, and has a remarkable promoting effect on plant disease resistance, plant growth and plant seed germination. The strain F019 and the AM fungus have the effect of mutually promoting colonization of the strain F019 and the AM fungus in the rhizosphere of the tobacco, and the combined application of the strain F019 and the AM fungus can obviously promote the growth of the tobacco and improve the resistance of the tobacco to the black shank.

The invention relates to the technical field of agricultural microbial fertilizers, in particular to a preparation method of a high-activity multifunctional foliar bacterial fertilizer, which comprises the following steps: preparing a composite culture medium, and accurately calculating the mass ratio of fish peptone to trehalose based on a target carbon nitrogen ratio; inoculating bacillus atrophaeus, bacillus siamensis and pseudomonas fluorescens in a specific proportion into the culture medium, and performing dynamic fed-batch fermentation; after fermentation, thalli are wrapped by forming protective colloid, and the addition condition of the microelement mother liquor is strictly controlled to protect the activity of the thalli; and finally, based on the feedback of the survival rate of the finished product thalli, intelligently adjusting the technological parameters of the next batch. The prepared foliar bacterial fertilizer has the advantages of being high in thallus activity, comprehensive in function, good in storage stability and the like, and upgrading from a fixed formula to self-adaptive process optimization is achieved.

The invention provides a strain of bacillus siamensis Bsiamensis and application thereof, the strain is classified and named as bacillus siamensis Bsiamensis, the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 35785, and the preservation date is September 1, 2025. The bacterial suspension, the fermentation liquor and the ethyl acetate extract of the fermentation liquor of the bacterial strain have good bacteriostatic activity on fusarium graminearum, fusarium oxysporum, fusarium laminatum, alternaria longibrachiata, colletotrichum gloeosporioides and colletotrichum frutescens.

TITLE: USE OF A BACILLUS SIAMENSIS STRAIN AS A BIOSOLUTION IN TOMATO CULTIVATION The present invention relates to the use of a particular bacterial species as a biosolution in tomato production and cultivation. More specifically, the invention relates to the use of a Bacillus siamensis strain, deposited with the National Collection of Microorganism Cultures (CNCM) under the order number CNCM I-5921, in stimulating the development and growth of tomato seeds and seedlings or improving tomato crop yields, and in improving the resistance and tolerance to abiotic stress of seeds, seedlings, and tomato crops. [Fig. 1]

The invention discloses a compound microorganism for preventing and treating chestnut fruit rot and application of the compound microorganism. The compound microorganism is composed of trichoderma atroviride, bacillus siamensis and bacillus velezensis, and is constructed by directionally screening strains with broad-spectrum inhibition effect on seven core pathogenic bacteria from Chinese chestnut fruit endophytes and verifying compatibility. The method has the advantages that the compound flora synergistically prevents and controls diseases through direct antagonism and induction of plant system resistance, and the defensive enzyme activity and the content of total polyphenol and total flavone of Chinese chestnuts are remarkably improved; according to the compound microorganism, the indoor control effect reaches 90% (the morbidity is reduced), the diseased fruit rates of Chinese chestnut fruits treated in a chestnut garden are reduced by 60% and 42.86% respectively after the Chinese chestnut fruits are stored for 30 days and 120 days, the effectiveness and the stability are obviously higher than those of a single strain, the compound microorganism can be introduced in the flowering period or sprayed in the storage period, use is convenient and flexible, and an efficient and stable biocontrol strategy is provided for green prevention and control of the Chinese chestnut fruit rot.

The invention discloses a bacillus siamensis fungicide, a biological selenium fertilizer and application of the bacillus siamensis fungicide and the biological selenium fertilizer in ecological restoration, and belongs to the technical field of soil salinization and heavy metal pollution prevention and control. A bacillus siamensis strain is screened from soil, and biological selenium particles which are uniform in particle size and rich in protein and polysaccharide functional groups on the surface are prepared in a culture system containing sodium selenite through biological reduction; then, the prepared biological selenium is independently applied or applied to plant rhizosphere or soil together with living bacillus siamensis to relieve salt-cadmium dual stress, and by adjusting the oxidation-reduction state and rhizosphere micro-ecology in the plant, Cdtoxicity is relieved, and the growth and physiological activity of the plant under salt-cadmium composite stress are improved. The prepared biological selenium is low in toxicity, good in stability, remarkable in synergistic effect with microorganisms, suitable for green remediation and efficient planting of salinized and heavy metal polluted farmlands and good in application prospect.

The invention relates to the technical field of microbial control, and provides bacillus siamensis and application thereof, the bacillus siamensis has been preserved in China General Microbiological Culture Collection Center (CGMCC) on June 17, 2025, and the preservation number is CGMCC No.34929. The bacillus siamensis has been applied to the field of microbial control. According to the technical scheme, the problem that no bacillus siamensis is applied to prevention and treatment of fish nocardia disease in the prior art is solved. Research finds that the bacillus siamensis provided by the invention has a very good effect in the aspect of biological prevention and control of fish nocardia disease, and a new strain resource is provided for biological prevention and control of fish nocardia disease, so that the bacillus siamensis has a good practical application value.

The invention discloses an alfalfa stress-resistant high-yield breeding method based on a microbiome regulation and control technology, and belongs to the technical field of microbial breeding. According to the breeding method, compound fertilizer doped with a microbial agent is applied in a seedling stage, a branching stage and a squaring stage respectively, and the compound fertilizer comprises a substrate and the microbial agent, the microbial agent is prepared from sinorhizobium meliloti, bacillus stearothermophilus, peste des petits ruminants, bacillus licheniformis, azotobacter brown, bacillus siamensis and bacillus amyloliquefaciens; the substrate comprises at least two of cow dung, pig manure, bean bran, corn straw and wood chips. According to the breeding method, the new microbial composition is prepared for preparing the compound fertilizer, the nutrition absorption capacity of the alfalfa can be improved through a microbial regulation function, and then the stress resistance and yield of the alfalfa are improved.

The application discloses a bacillus siamensis strain and application of the bacillus siamensis strain in fermentation of rapeseed meal. Bacillus siamensis The application provides the bacillus siamensis (BSJ-2) strain with a preservation number of CGMCC No.32638. The strain or culture solution or bacteria liquid or fermentation liquor thereof is applied to any one of the following or preparation of any one of the following products: 1) degradation of rapeseed meal storage protein; 2) improvement of rapeseed meal nutritional quality; 3) improvement of rapeseed meal protein utilization rate; the application finds that the bacillus siamensis (BSJ-2) strain can degrade rapeseed meal storage protein, improve rapeseed meal nutritional quality and improve rapeseed meal protein utilization rate. Bacillus siamensis The fermentation product of the bacillus siamensis (BSJ-2) strain can be used for preparing animal feed.

The invention relates to the technical field of compost and microbial inoculum preparation, and provides a method for preparing compound bacteria through high-temperature rapid compost maturing of sugarcane leaves and cow dung, and the method comprises the following steps: step 1, raw material pretreatment: crushing the sugarcane leaves to particle size, drying the cow dung to water content, mixing the sugarcane leaves and the cow dung according to a mass ratio of 3: 1-5: 1, and sterilizing; step 2, preparing an initial bacterial liquid: inoculating a composite strain into a culture medium, and culturing for 24-48 hours at 35-40 DEG C under the condition that the rotating speed of a shaking table is 150-180rpm to obtain the initial bacterial liquid; the composite strains comprise bacillus siamensis and bacillus pumilus, and the ratio of the strains is 2: 1; and step 3, high-temperature composting and maturing. Agricultural waste recycling is achieved by treating sugarcane leaves and cow dung, pollution is reduced, and low-cost raw materials are provided; a dynamic temperature control and forced ventilation process and a specific strain are adopted, so that decomposition is accelerated, and the activity of the microbial inoculum is improved; the system achieves modularized operation, achieves whole-process standardization and automatic control, and improves the efficiency and stability.

TITLE: USE OF A BACILLUS SIAMENSIS STRAIN AS A BIOSOLUTION IN TOMATO CULTIVATION The present invention relates to the use of a particular bacterial species as a biosolution in tomato production and cultivation. More specifically, the invention relates to the use of a Bacillus siamensis strain, deposited with the National Collection of Microorganism Cultures (CNCM) under the order number CNCM I-5921, in stimulating the development and growth of tomato seeds and seedlings or improving tomato crop yields, and in improving the resistance and tolerance to abiotic stress of seeds, seedlings, and tomato crops. [Fig. 1]

The invention relates to bacillus siamensis and an application of the bacillus siamensis in aquaculture. The bacillus siamensis is obtained by carrying out antagonistic strain separation and screening by taking vibrio parahaemolyticus as an indicator bacterium. Through verification, the strain has a relatively obvious inhibition effect on vibrio parahaemolyticus causing vibriosis in aquaculture, also has certain resistance to pathogenic bacteria such as vibrio alginolyticus and aeromonas hydrophila, in addition, the strain has relatively good high-temperature resistance, acid and alkali resistance and salt tolerance, and when the strain or a microbial agent prepared from the strain is applied to aquaculture, the yield of aquaculture is greatly increased. The compound has high inhibition rate on various germs including vibrio parahaemolyticus in a water body, does not influence the water body environment, has potential effects of promoting the growth of bred animals, stimulating the response of an immune system, improving the immunity, improving the water quality condition, repairing the ecological environment and the like, and has a wide application prospect.

The application discloses a kind of for preventing and treating chestnut fruit rot disease complex microorganism and its application.The application complex microorganism is composed of Trichoderma atroviride and Bacillus siamensis, Bacillus velezensis, by from chestnut fruit endophyte directional screening to seven kinds of core pathogenic bacteria have broad-spectrum inhibitory effect strain, is constructed after compatibility verification.It is superior in that: complex microbial population is synergistically controlled disease by direct antagonism and induced plant system resistance, significantly improves chestnut defense enzyme activity and total polyphenol, total flavone content;Indoor control efficiency reaches 90% (reduces the incidence), chestnut fruit in chestnut orchard treatment is reduced 60% and 42.86% respectively in 30 days and 120 days, effective and stability are obviously stronger than single strain, and the application complex microorganism can be introduced by flowering period or sprayed in storage period, and is convenient and flexible, provides efficient, stable biocontrol strategy for chestnut fruit rot green control.

The present application relates to the technical field of biocontrol bacteria, and particularly relates to a bacillus siamensis (Bacillus siamensis) ARF-SR1, a biocontrol bacterium and application thereof. Bacillus siamensis The present application provides the preservation number of the bacillus siamensis ARF-SR1 as CGMCC No.33715. The bacillus siamensis ARF-SR1 provided by the present application is a biocontrol strain isolated from a healthy alfalfa field in Ningxia, has a significant inhibitory effect on alfalfa root rot pathogenic bacteria (especially Fusarium solani, Fusarium oxysporum and Phoma sp.), an antagonistic rate of 57.71-68.86%, can reduce the incidence of alfalfa root rot, enriches the biocontrol bacteria resource library, and is suitable for green control of alfalfa root rot.

The invention discloses a compound microbial fertilizer and a preparation method thereof, and the compound microbial fertilizer is prepared from the following raw materials in parts by weight: 100-150 parts of a bacillus siamensis microbial agent, 150-200 parts of a streptomyces pourpuensis microbial agent, 130-175 parts of a synergist, 185-215 parts of an auxiliary material, 110-140 parts of a chelate material, 41-78 parts of a catalyst, 10-30 parts of an active agent, 1-3 parts of an inhibitor, 0.5-1.5 parts of a thickening agent and 100-150 parts of water. The preparation method of the compound microbial fertilizer is simple and easy to implement, all the materials are added according to a specific sequence, it can be guaranteed that the materials are fully dissolved and mixed, the stability and uniformity of the fertilizer are guaranteed, and industrial production is facilitated; by applying the compound microbial fertilizer disclosed by the invention, the problem of lack of trace elements of crops can be directly solved, the growth and development of the crops are comprehensively optimized through the synergistic interaction of amino acids, the goals of quality improvement, yield increase, disease resistance and environmental protection are achieved, and the compound microbial fertilizer conforms to the development requirements of green, high-end and efficient agriculture and has wide popularization and application prospects.

This invention discloses a strain of *Corynebacterium lucida* CYPJ1 and its applications. The *Corynebacterium lucida* CYPJ1 has the preservation number CGMCC No. 35719, and its saccharifying enzyme activity is 73.19 ± 0.09 U / mL. Using homogenized raw materials and fermentation with this strain results in increased soluble dietary fiber content and enhanced physical and functional properties. Foods or health products fermented with homogenized raw materials and this strain have lipid-lowering effects. This invention also found that using *Corynebacterium lucida* (…) after homogenization of raw materials… Clavispora lusitaniae CYPJ1 and Kluyveromyces macrocephala ( Kluyveromyces marxianus SXJ2 or Bacillus sicca ( Bacillus siamensis The DL-RXL01 combination fermentation yields even better results.

The invention discloses bacillus siamensis and application thereof, and belongs to the technical field of microorganisms. The bacillus siamensis EfA-3 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 13, 2024, and the preservation number is CGMCC NO.31710. The bacillus siamensis EfA-3 is a bacillus siamensis EfA-3. Experiments prove that the strain EfA-3 simultaneously has the functions of inhibiting five plant pathogenic fungi (phytophthora nicotianae, fusarium oxysporum, alternaria alternata, sclerotium rolfsii and fusarium graminearum) and three plant pathogenic bacteria (ralstonia solanacearum, carrot soft rot solidobacterium and citrus canker pathogen) and poison-killing prodenia litura, so that the strain EfA-3 has the effects of inhibiting the five plant pathogenic fungi (phytophthora nicotianae, fusarium oxysporum, alternaria alternata, sclerotium rolfsii and citrus canker pathogen). The technical blank of multifunctional biocontrol of bacillus siamensis is filled, and a new resource is provided for development of a low-cost biological control preparation.

The application discloses a kind of rotten agent with disease prevention and control function and preparation method and application, belong to the cross field of agricultural microorganism technology and biological control.It is composed of compound bacteria powder and microcapsule fungicide by the rotten agent, the compound bacteria powder is made of bacillus subtilis ZKY01 bacteria powder and bacillus velezensis ZKY02 bacteria powder, and the microcapsule fungicide is bacillus siamensis ZKY03 bacteria powder embedded in microcapsule carrier;Wherein, the bacteria powder of three kinds of bacteria is the effective viable count not less than 1×10 11 CFU / g powder obtained by fermentation culture.The application can shorten the rotting period of agricultural organic waste such as straw, livestock and poultry manure to 12~15 days through the synergistic effect of three strains, and effectively prevent and control tobacco root black rot, black foot disease and other soil-borne diseases through the secretion of antibacterial substances, competition for nutrients and other mechanisms.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus siamensis and application thereof in prevention and treatment of tobacco bacterial wilt. The bacillus siamensis XZ92 is preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus siamensis XZ92 is CCTCC NO: M 2025801. The strain shows a strong specific antagonistic effect on ralstonia solanacearum, the prevention effect of a pot experiment reaches 47.46%, the strain has no pathogenic risk on tobacco, the strain XZ92 is separated from tobacco and potato intercropping soil, is natural, has strong colonization capability on target ecological niche, has strong spore structure stress resistance, and can stably survive and continuously play a role in continuous cropping soil.

The present invention relates to the use of a particular bacterial species as a biosolution in the production and cultivation of tomatoes. More particularly, the invention relates to the use of a Bacillus siamensis strain, deposited with the National Collection of Cultures of Microorganisms (CNCM) under the accession number CNCM I-5921, in the stimulation of the development and growth of seeds and of tomato plants or the improvement of the yields of tomato crops, and in the improvement of the resistance and tolerance of seeds, tomato plants and tomato crops to abiotic stress.