The invention discloses a prevention and treatment method of
bacillus siamensis in pine
shoot disease, QX3-4 has relatively strong bacteriostatic activity on the pine
shoot disease of
masson pine, the bacteriostatic rates can reach 74.38%, 80.1% and 85.17% respectively when
bacteria are inoculated for 0 hour, 24 hours and 48 hours later, and the bacteriostatic rate can reach 100% when the
bacteria are inoculated for 72 hours later, so that the
bacillus siamensis can be used for preventing and treating the pine
shoot disease of
masson pine, and the
bacillus siamensis can be used for preventing and treating the pine shoot disease of
masson pine. The invention relates to the technical field of biocontrol
bacteria. According to the prevention and treatment method of the bacillus siamensis in the pine
wilt disease, the sterile
fermentation filtrate of the QX3-4 still has an inhibition effect on the aschersonia championii when being diluted by 100 times, the bacteriostasis rate is kept at 90% or above when being diluted by 50 times or below, the bacteriostasis rate can reach 87.49% when the sterile
fermentation filtrate of the QX3-4 is diluted by 100 times after all components of a culture medium are optimized, and in a
potting prevention effect test, the bacteriostasis rate of the sterile
fermentation filtrate of the QX3-4 can reach 87.49%. The QX3-4 can relieve the disease symptom of the
pinus massoniana seedling pine wilt in the early stage and basically stop the disease development in the later stage, and the bacterial suspension, the fermentation liquor and the fermentation filtrate of the QX3-4 have prevention and treatment effects on the aschersonia confusa.