117 results about "Stem rot" patented technology

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention discloses a multifunctional talaromyces sp. Fungus RB-2 for preventing and controlling ginger stem rot and application of the multifunctional talaromyces sp. Fungus RB-2, and belongs to the technical field of microorganisms. The talaromyces sp. Fungus RB-2 is preserved in the China Center for Type Culture Collection, the preservation number of the talaromyces sp. Fungus RB-2 is CCTCC NO: M 20251722, and the preservation date of the talaromyces sp. Fungus RB-2 is July 28, 2025. The talaromyces sp. Strain RB-2 with the effect of inhibiting the growth of pathogenic bacteria is separated and purified, and the strain RB-2 has remarkable antagonistic activity on ginger stem rot pathogenic bacterium group pythium aphanidermatum and various economic crop pathogenic bacteria and has the potential of being developed into a biocontrol inoculant. The talaromyces sp. Strain RB-2 also has phosphorus dissolving and growth promoting capabilities, can remarkably promote the growth of ginger, and has the potential of preparing a soil conditioner and a microbial fertilizer, so that the micro-ecology of soil is improved, and the productivity of crops is enhanced.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

The invention relates to the technical field of microorganisms, in particular to penicillium LDFST10 and application of the penicillium LDFST10 in promoting plant growth or preventing and treating stem rot. The preservation number of the penicillium LDFST10 is CGMCC (China General Microbiological Culture Collection Center) No. 41960. The application comprises the step of applying the penicillium LDFST10 or the fermentation product thereof to rhizosphere soil of the plant so as to promote the growth of the plant and prevent and treat basal stem rot. The invention researches and screens out the penicillium frutescens LDFST10 capable of promoting plant growth and inhibiting various pathogenic bacteria of stem rot, various growth promoting preparations or bacteriostatic agents or drugs aiming at the stem rot can be developed on the basis of the penicillium frutescens, and the penicillium frutescens LDFST10 has important value in the planting field of various crops such as corn, wheat and the like.

Methods and compositions for identifying, selecting and / or producing a pathogen resistant plant, plant cell or seed (e. g,, a maize plant, plant cell or seed) are provided. Polynucleotides and polypeptides are provided, which when expressed in a plant increase disease and / or pathogen resistance (e.g., to Fusarium stalk rot) of the plant. Plants, plant cells and seed are provided comprising such polypeptides and polynucleotides, as are methods for their use.

The invention belongs to the field of plant biotechnology and plant molecular breeding, and particularly relates to a brassica napus BnabZIP1 gene and application thereof in prevention and control of sclerotinia rot of colza. According to the invention, a gene editing technical vector is constructed aiming at sgRNA of the BnabZIP1 gene, agrobacterium tumefaciens is transformed, then brassica napus is transfected, and a homozygous mutant plant after the BnabZIP1 gene is knocked out has stronger sclerotiniose resistance. Results show that the BnabZIP1 gene can be used as a molecular marker or a genetic engineering improvement target of sclerotiniose resistance of rape, is used for screening and creating disease-resistant rape varieties, and has a good molecular breeding application prospect.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

The invention relates to a fluazinam-containing pesticide composition, a seed treatment suspending agent as well as a preparation method and application of the seed treatment suspending agent. The invention provides a fluazinam-containing pesticide composition. The active ingredients of the fluazinam-containing pesticide composition comprise fluazinam, fludioxonil and clothianidin, the mass ratio of the clothianidin to the fluazinam to the fludioxonil is (6-7): (0.4-0.8): (0.2-0.6), and the composition has an excellent synergistic effect. The suspending agent for seed treatment is compounded with a film-forming agent composed of acrylate and polydimethylsiloxane according to a specific proportion, it can be ensured that seed coats are not prone to falling off after being coated, meanwhile, adjustment can be conducted according to environmental adaptability, a coating layer is evenly mixed with a pesticide and pigment, the release time of effective components of pesticides is prolonged, and the pesticide effect is improved. The problem of seed coating fading is effectively solved, and the pesticide composition has an excellent effect in prevention and treatment of agricultural diseases such as peanut root rot, grub, wheat stem rot and aphid.

The invention belongs to the technical field of molecular markers, and particularly relates to a QTL gene for regulating and controlling maize fusarium graminearum stem rot resistance, a KASP molecular marker and application of the QTL gene and the KASP molecular marker. A maize high-disease-resistance inbred line H1710 and a high-susceptibility inbred line Huangzao 4 are used for constructing an F2 group, and high-disease-resistance and high-susceptibility samples are selected from the F2 group for constructing a mixed pool for BSA-seq analysis; in combination with bioinformatics analysis, molecular markers and fine positioning, a stable QTL gene for controlling the resistance of the fusarium graminearum stem rot of the corn is positioned from the sixth chromosome bin6.01 of the corn. On the basis, the invention provides a corn genome region capable of improving the corn stem rot resistance and a corresponding DNA (Deoxyribose Nucleic Acid) fragment thereof. In addition, the invention also provides a nuclear KASP molecular marker linked with the QTL locus. After the molecular marker is screened and verified, the SNP molecular marker provided by the invention can be used for efficiently and quickly identifying the genotype of a to-be-detected corn material and screening a stem rot resistant material.

The invention discloses application of a glutathione transferase gene EuGST in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. The invention discloses an application of a glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. A nucleotide sequence of the glutathione transferase gene EuGST is shown as SEQ ID NO.1. The invention also discloses an application of the glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. Fusarium graminearum is inoculated to ears and stems of an overexpressed EuGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the maize inbred line is improved; fusarium graminearum is inoculated to ears and stems of the overexpressed EuGST transgenic hybrid, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn hybrid is improved.

This invention discloses an oxyindene compound containing substituted pyridine and its application as a fungicide. The oxyindene compound containing substituted pyridine has broad-spectrum fungicidal activity, especially effective in controlling rice sheath blight, apple tree rot, rapeseed sclerotinia stem rot, wheat scab, pepper blight, cucumber anthracnose, tomato gray mold, corn curvature blight, rice blast, tomato early blight, wheat take-all, and wheat stem rot. It can be used as a fungicide for the prevention and control of the above-mentioned plant diseases.

The invention relates to the technical field of biology, and particularly discloses application of ZmbHLH67 protein or a coding gene thereof in regulating and controlling stem rot resistance of plants. The research finds that the ZmbHLH67 gene can positively regulate and control the disease resistance of stem rot. Therefore, the invention provides the application of the ZmbHLH67 protein or the coding gene thereof, or a biological material containing the coding gene thereof in regulating and controlling the stem rot resistance of plants. The invention provides a new method for improving the stem rot resistance of plants, and a new path is opened up for creating disease-resistant plant varieties. The method can effectively enhance the disease resistance of plants and improve the growth state and production performance of the plants, and has important application value for promoting agricultural production and improving the yield and quality of crops.

This invention discloses a nanoRNA delivery system and its application in the control of Sclerotinia sclerotinia disease, relating to the field of biotechnology. The preparation method of the nanoRNA delivery system includes the following steps: mixing chitosan, sucrose, and aspartic acid uniformly and then subjecting them to a hydrothermal reaction to obtain a quantum dot-chitosan degradation product complex; mixing the quantum dot-chitosan degradation product complex with double-stranded RNA to obtain the nanoRNA delivery system; the double-stranded RNA is used to silence Sclerotinia sclerotinia virulence-related transcription factor genes. The nanoRNA delivery system provided by this invention can effectively protect RNA and exhibits a very high inhibitory effect on Sclerotinia sclerotinia in plants, providing a novel technical solution for the green and precise control of Sclerotinia sclerotinia disease.

This invention discloses a fungicide composition containing WML-01 and triazole compounds. The fungicide composition is characterized by comprising WML-01 and triazole compounds in a mass ratio of 10:1 to 1:10. The fungicide composition provided by this invention, with WML-01 and triazole agents as active ingredients, exhibits a synergistic effect in the control of rice bakanae disease, wheat stem rot, wheat scab, plant root rot, and rapeseed sclerotinia stem rot. It can effectively improve disease control efficacy, reduce pesticide usage, delay the development of pesticide resistance, and increase crop yield. It is safe, effective, and economical, and has broad market application prospects.

The present application provides a kind of streptomyces, named Streptomyces sp., is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No:65917.The present application also provides fermentation broth of the streptomyces and ethanol extract and crude extract of fermentation broth thereof.The new species of streptomyces and its crude extract and the like of the present application have broad-spectrum antibacterial activity, have good antagonism to many pathogenic bacteria such as banana long-shaped spot fungus and banana anthracnose fungus, and can make banana anthracnose fungus hyphae wrinkle, deform, break, inhibit banana anthracnose fungus spore germination, make banana anthracnose fungus spore germination wrinkle, break, inhibit banana anthracnose fungus growth, and can also improve peroxidase, polyperoxidase, CAT activity and the like in banana peel, is a potential biological agent for preventing and treating anthracnose disease, stem rot disease and the like, has broad development space, and has good development and application prospect.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide fertilizer for preventing and treating corn stem rot. The invention relates to a pesticide active component. The pesticide active component is prepared by compounding ifen trifluconazole and pydiflumetofen according to a mass ratio of (1-9): (9-1). According to the invention, the ifen trifluconazole and the pydiflumetofen are compounded into a pesticide active component with a bactericidal effect, so that the prevention and treatment effect on the corn stem rot can be improved, the drug resistance risk of the pesticide can be reduced, and a support can be provided for developing a novel efficient compound bactericide for preventing and treating the corn stem rot. In addition, the functional pesticide-fertilizer granules are formed by combining pesticide active ingredients with the fertilizer, have the pesticide effect and the fertilizer effect, have the pesticide effect and the fertilizer effect through one-time application, and have good application prospects.

The application belongs to the field of plant biotechnology and plant molecular breeding, and particularly relates to Brassica napus BnabZIP1 Gene and application thereof in prevention and control of oilseed rape sclerotinia stem rot BnabZIP1 The sgRNA of the gene is used to construct a gene editing vector, and the agrobacterium is transformed, then the Brassica napus is transfected, and it is found that BnabZIP1 The homozygous mutant plant after the gene knockout has stronger resistance to the sclerotinia stem rot. BnabZIP1 The gene can be used as a molecular marker or a genetic engineering improvement target for the resistance to the sclerotinia stem rot, is used for screening and creating the disease-resistant oilseed rape varieties, and has a good molecular breeding application prospect.

The invention relates to an RPA-CRISPR / Cas kit and method for detecting fusarium oxysporum, and belongs to a molecular detection technology, and an RPA-CRISPR / Cas system adopts an RPA primer pair and an sgRNA sequence of the following sequence information: FspoF: 5 '-TTCCATGCTGGTACCGCTGAACAGGTATAGC-3', SgRNA: SgRNA, SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: SgRNA: According to the present invention, with the application of the Fspo-sgRNA3, the FspoF: 5 '-GAGGTTGTCTCTTCCTGCGCTGAATG-3', and the Fspo-sgRNA3: 5 '-GUCUAAAGGACGGUGUGCAAAGCCGGUGGUGGUGGUGGUUGGUGGUUGGUGGUAGGAGA-3', the sample suspected to be infected with the Fusarium oxysporum can be rapidly detected on site, the detection sensitivity can achieve 30.3 * 10 <-6 > ng / [mu] L, and the important significance is provided for early detection of the infection of the corn stem rot pathogenic bacteria, prevention of the spread of the pathogenic bacteria infection, and accurate adoption of the plant

This application discloses a strain of *Priestia taiwanensis* W178 and its application in controlling soil-borne fungal diseases. This strain was deposited at the China Center for Type Culture Collection (CCTCC) on December 29, 2025, with accession number CCTCC NO:M 20253047. The strain exhibits significant antagonistic activity against 11 soil-borne pathogenic fungi, including *Sclerotinia sclerotiorum* and *Fusarium graminearum*, with an inhibition rate reaching up to 91.10%. This application also establishes a fermentation process for this strain, resulting in a spore count of 19.3 × 10⁻⁶ after fermentation. 8 CFU / mL. Pot experiments showed that the fermentation broth, diluted 10 times, was significantly effective in controlling rapeseed sclerotinia stem rot and could effectively inhibit the spread of lesions. This application is the first to discover the broad-spectrum biocontrol potential of *Priscilla taiwanensis* and provides an active strain and its fermentation product that can be developed into microbial agents, bio-organic fertilizers, or compound microbial fertilizers, suitable for the green control of various soil-borne fungal diseases such as rapeseed sclerotinia stem rot and wheat stem rot.

The application discloses a detection primer and probe for detecting a pathogenic bacterium of a crop stem base rot and a chip digital PCR detection method. TEF-1 alpha The application takes a pathogenic bacterium of a crop stem base rot as a target, and the pathogenic bacterium is a Fusarium pseudograminearum Tri Core gene cluster nucleic acid fragments are respectively designed to obtain high-specificity detection primer pairs and probes, and a double-chip digital PCR quantitative detection method for a dominant pathogenic bacterium of a wheat stem base rot is established according to the same. The correlation coefficients of ideal copy numbers and actual copy numbers of target samples detected by the double-chip digital PCR quantitative method are all greater than 0.9998, the target DNA can be more accurately detected, the double-chip digital PCR quantitative method shows higher accuracy and sensitivity than fluorescence quantitative PCR in a complex rhizosphere soil sample, and the double-chip digital PCR quantitative method has application prospects in accurate quantification and detection of nucleic acids of the dominant pathogenic bacterium of the wheat stem base rot.

The invention relates to the field of biocontrol of plant diseases. The invention provides a bacillus safensis strain HMQAU23223, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 13, 2025, and the preservation registration number is CGMCC No.36623. The bacillus safensis strain HMQAU23223 has the advantages that the preservation number is CGMCC No.36623; the invention also provides a biocontrol inoculant. The invention also provides a preparation method of the biocontrol inoculant. The invention further provides the application of the bacillus safensis strain HMQAU23223 or the biocontrol microbial inoculum or the biocontrol microbial inoculum prepared by the preparation method. The invention further provides the application of the bacillus safensis strain HMQAU23223 or the biocontrol microbial inoculum prepared by the preparation method. The invention further provides a method for preventing and treating plant diseases. The invention also provides an application of the bacillus safensis strain HMQAU23223 in preparation of a compound for inhibiting phytopathogen or preventing and treating plant pathogenic nematodes. The fermentation filtrate of the bacillus safensis strain HMQAU23223 provided by the invention has the lethal effects of 89% or above on pine wood nematode, meloidogyne incognita, wheat cyst nematode, heterodera filipjevi and ditylenchus destructor, and has better biocontrol potential.

This invention discloses the application of the protein encoded by the ZmDDRPβ gene in regulating maize stalk rot resistance and a method for cultivating maize plants. The protein encoded by the ZmDDRPβ gene not only negatively regulates maize stalk rot resistance but also negatively regulates maize salt tolerance. By reducing the expression level of the protein encoded by the ZmDDRPβ gene, maize stalk rot resistance is improved while simultaneously increasing maize's tolerance to salt stress. This reduces the severity of stalk rot outbreaks and minimizes yield loss. Simultaneously, it enables maize to grow and develop normally in saline-alkali soils. Therefore, the stalk rot-resistant and salt-tolerant maize plants cultivated are more suitable for maize stalk rot and severely saline-alkali soils. This gene provides a clear genetic improvement target for molecular breeding of maize with disease resistance and salt tolerance, offers new gene resources for cultivating more high-quality maize varieties adapted to different stalk rot resistance and saline-alkali environments, and provides new insights into the research of plant disease resistance and salt tolerance mechanisms.

Provided is a method for increasing plant disease resistance. Specifically, provided is a method for increasing plant disease resistance by means of reducing or inhibiting the expression level and / or activity of the SWEET11a gene or a protein encoded thereby. The method can increase plant disease resistance, especially increase the resistance to gray leaf spot, leaf blight, bacterial wilt and stem rot, and has important application value in biological breeding.

This invention relates to the field of microbial technology and discloses a biocontrol strain for controlling peanut stem rot, wherein the biocontrol strain is *Bacillus pilaris* (…). Paenibacillus peoriae This invention also provides a biocontrol agent containing this strain and its preparation method. Genomic analysis revealed that this strain contains gene clusters encoding active substances such as fusarium and polymyxin, exhibiting significant antagonistic activity against peanut stem rot fungus, and broad-spectrum inhibitory activity against various plant pathogenic fungi, including wheat stem base rot fungus and rapeseed sclerotinia. Furthermore, this strain possesses the ability to secrete auxin, produce proteases, and produce cellulases. The prepared inoculum, applied through root irrigation, can construct a preventative biological barrier in the rhizosphere, providing both disease prevention and growth promotion effects. This invention provides a highly efficient germplasm resource for the green control of crop diseases.

The invention discloses paenibacillus polymyxa as well as a construction method and application thereof. Antagonistic bacteria are screened from soil suffering from wheat stem rot, a paenibacillus mucilaginosus strain with broad-spectrum bacteriostasis and prevention and treatment effects is screened out through enrichment culture, the paenibacillus mucilaginosus strain is named as Paenibacillus polymyxa ZDW-24-15 and preserved in the China Center for Type Culture Collection on March 10, 2025, the address is No. 299 on eight road in Wuchang District, Wuhan City, China, and the preservation number is CCTCC NO: 2025412. According to the present invention, the recombinant ZDW-24-15-[delta] abrB bacterial strain is further obtained by carrying out gene modification on the ZDW-24-15 bacterial strain, and the recombinant ZDW-24-15-[delta] abrB bacterial strain shows the more excellent antibacterial property than the wild type ZDW-24-15 bacterial strain.

The invention discloses an application of corn E3 ubiquitinase in enhancing the resistance of corn to corn stem rot and drought. The name of the corn E3 ubiquitinating enzyme is ZmWIPF2, and the corn E3 ubiquitinating enzyme is shown as a sequence 2 in a sequence table. When the corn is subjected to abiotic stress such as drought and biological stress such as stem rot, the ZmWIPF2 cooperates with related genes to regulate and control the corn to resist the stem rot and drought and respond to pathogenic bacterium infection and drought stress. The ZmWIPF2 gene contains a zinc finger structural domain and an RING-H2 structural domain, wherein RING-Ubox is an E3 ubiquitin ligase. Transgenic material verification proves that the gene can improve the stress resistance of corn, has important significance for breeding stress-resistant corn varieties, and is suitable for wide popularization.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying wheat stem rot resistance and application of the SNP molecular marker. The invention belongs to the technical field of biology, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker for identifying wheat stem rot resistance and application of the SNP molecular marker. The substance for detecting the polymorphism or genotype of the SNP site in the wheat genome can be applied to any one of the following aspects: (1) identifying or assisting in identifying the wheat stem rot resistance; (2) wheat breeding; (3) preparing a product for identification or auxiliary identification of wheat stem rot resistance; (4) preparing a wheat breeding product; the SNP site is an SNP site on a wheat 2B chromosome, the nucleotide type of the SNP site is A or G, and the SNP site is the 24th nucleotide of a sequence 1 in a sequence table. The substance for detecting the polymorphism and genotype of the SNP site can be combined with other substances to prepare a product for identifying a wheat variety with wheat stem rot resistance, and the product is applied to wheat breeding.