304 results about "Stem rot" patented technology

The invention discloses a microbial agent for preventing and treating soil-borne diseases of food crops and a preparation method of the microbial agent, and belongs to the technical field of microorganisms. The microbial inoculum comprises three functional strains, namely pseudomonas guayensis, paenibacillus polymyxa and trichoderma koningiopsis, the three strains are mixed in equal proportion and cooperate with one another, so that the microbial inoculum has a remarkable disease-resistant effect, has an antagonistic effect on various diseases of wheat and corn, can be used for preventing and treating wheat root rot, take-all and banded sclerotial blight as well as corn stem rot and banded sclerotial blight, and has a wide application prospect. Particularly, the inhibition effect on the take-all disease of wheat is optimal. Meanwhile, the coating agent and the auxiliaries are used, so that the acting durability and stability of the microbial inoculum are improved. The microbial agent is matched with a conventional base fertilizer for use, use of chemical bactericides is reduced, growth of common pathogenic bacteria of food crops is effectively inhibited, and the yield of the food crops is increased.

The invention relates to the field of fertilizers, in particular to a stable fertilizer for preventing and treating peanut root rot and a preparation method thereof, and the fertilizer comprises a fertilizer inner core layer, a nano microbial metabolite layer and a microbial protective layer from inside to outside. According to the stable fertilizer for preventing and treating peanut rhizome rot, the capsule embedding inhibitor made of humic acid and the trichoderma aureoviride metabolite are adopted to construct a dual nitrification inhibition system, and the capsule embedding inhibitor and the trichoderma aureoviride metabolite have a synergistic effect, so that the nitrification effect is reduced, the nitrogen fertilizer utilization rate is increased, and non-point source pollution is reduced.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus velezensis and application thereof. The bacillus velezensis strain is separated and screened from rhizosphere soil of wheat in a main wheat area in Hebei Province, the growth characteristics and physiological and biochemical characteristics of the bacterial colony are identified, then 16S rDNA sequencing is carried out, Blast homology comparison is carried out in a GenBank database, the bacterial strain is identified to be bacillus velezensis through the analysis, and the bacillus velezensis strain is identified to be bacillus velezensis. The fungicide containing the bacillus velezensis has a good prevention and treatment effect on wheat stem rot through seed coating.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention discloses bacillus velezensis and application of the bacillus velezensis in prevention and treatment of basal stem rot, the classification name of the bacillus velezensis is bacillus velezensis GN-22, the bacillus velezensis is preserved in China Center for Type Culture Collection (CCTCC), the preservation number of the bacillus velezensis is CCTCC NO: M2025566, and the preservation date is March 24, 2025. The bacillus velezensis GN-22 disclosed by the invention belongs to rhizosphere growth-promoting bacteria, has a remarkable inhibition effect on different pathogenic fungi such as fusarium graminearum, fusarium pseudograminearum, fusarium asiaticum, fusarium verticillioides and fusarium oxysporum, and plays an important role in biological control of diseases such as corn and wheat stem rot; meanwhile, the strain has a growth promoting effect and can promote corn growth. The strain provided by the invention has the advantages of simple culture conditions, easiness in preservation, easiness in industrial production and good development and application prospects.

The present invention relates to the technical field of molecular markers for maize resistance to stalk rot and genetic breeding, and in particular, to a QTL related to maize resistance to stalk rot, SNP molecular markers closely linked with the QTL, and a use of the SNP molecular markers. Provided is a QTL related to maize resistance to stalk rot, wherein the QTL is located on maize chromosome 10. Also provided are SNP molecular markers Ks4 and Ks6 closely linked with a QTL site for maize resistance to stalk rot. By detecting the SNP molecular markers, the genotype of a maize material under test can be accurately identified in a high-throughput manner, a material resistant to stalk rot is selected, and phenotype identification and assisted screening of the maize resistance to stalk rot can also be carried out in a seedling stage, thus significantly reducing production cost and significantly enhancing the efficiency of breeding maize resistant to stalk rot.

The invention discloses application of a ZmFS1 gene in corn disease prevention and control, and belongs to the technical field of crop disease prevention and control. The nucleotide sequence of cDNA of the ZmFS1 gene is as shown in SEQ ID NO.1. By inhibiting expression of the gene, resistance of corn to various diseases including southern leaf blight, northern leaf blight, southern rust disease, anthracnose, stem rot, seed rot and the like can be remarkably enhanced, a new solution thought is provided for preventing and treating corn diseases from the perspective of molecular biology, and the application prospect is broad. The method can be applied to corn disease-resistant breeding.

The invention discloses application of a compound of paenibacillus polymyxa and a chemical bactericide in preparation of a medicament for preventing and treating wheat stem rot. Belongs to the technical field of plant disease control. The invention studies the control effect of compound seed dressing treatment of paenibacillus polymyxa NPDY05-8 and seven common bactericides on the growth condition of wheat in the seedling stage and the control effect of wheat stem rot in the seedling stage, and the result shows that the compound seed dressing treatment of paenibacillus polymyxa NPDY05-8 and the bactericides can effectively improve the control effect of the bactericides on wheat stem rot; and compared with wheat treated by a single bactericide, wheat treated by mixed seed dressing of the bactericide and the paenibacillus polymyxa NPDY05-8 is thicker in leaf color and thicker in stalk, has the effects of promoting rooting and growth, and has huge application potential.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention provides application of a pseudomonas parabarium YC15 strain in efficient prevention and treatment of wheat stem rot and various pathogenic bacteria. The invention provides a new application of the pseudomonas parabarium YC15 strain in prevention and control of plant diseases, and research shows that the YC15 strain has broad-spectrum bacteriostatic activity, has remarkable inhibitory activity on various plant pathogenic bacteria, can inhibit pathogenic fungi such as wheat stem rot, sesame root rot and colletotrichum gloeosporioides, and has a wide application prospect in prevention and control of plant diseases. And the compound has a relatively good inhibition effect on pathogenic bacteria, namely sesames pseudomonas solanacearum. The YC15 strain is applied to prevention and control of wheat stem rot, and pot experiments show that after the YC15 strain is adopted for treatment, plant lesions can be reduced, the inhibition effect of pathogenic bacteria on plants can be broken, the pathogenic bacteria can be effectively inhibited, plant growth can be promoted, and the wheat stem rot can be efficiently prevented and controlled. The invention provides new application of the YC15 strain, and the YC15 strain can be used for preventing and treating various plant protobacteria and diseases thereof, and has good development and application potential as a biocontrol bacterium.

The invention provides a Trichoderma asperellum TAZ19, a biocontrol inoculant and application of the Trichoderma asperellum TAZ19, the Trichoderma asperellum TAZ19 is preserved in the China Center for Type Culture Collection on October 25, 2024, and the preservation number of the Trichoderma asperellum TAZ19 is CCTCC M 20242341. The active ingredients of the biocontrol inoculant comprise the trichoderma asperellum TAZ19 strain, and the trichoderma asperellum TAZ19 strain is one or more of conidia, hypha, fermentation filtrate or active extracts of the trichoderma asperellum TAZ19 strain of the trichoderma asperellum TAZ19 strain. The trichoderma asperellum TAZ19 has an excellent antagonistic effect on a plurality of plant disease pathogenic bacteria such as dispora pinnatifida, a plurality of fusarium oxysporum, apple black beancurd skin fungi and botryosphaeria dothidea, has broad-spectrum antibacterial activity, is high in growth speed, high in antibacterial activity and high in hyperparasitism ability, has better saline-alkaline tolerance, also has a growth promoting effect on plants, and can be widely applied to the field of plant diseases. The strain can be used as an active component of a biocontrol inoculant to be widely applied to prevention and treatment of various plant diseases such as pine tree branch rot, wheat stem rot and tomato fusarium wilt, and has a wide application prospect.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to Lecanicillium lecanii as well as a fungicide and application thereof, Lecanicillium lecanii GC-2 is obtained by screening and separating phyllospheric microorganisms, and the strain is proved to have relatively strong inhibitory activity on wheat stripe rust, fusarium graminearum and pseudocereal fusarium; a pot experiment verifies that the strain has a very strong inhibition effect on uredospores of wheat stripe rust, has a relatively strong antagonism effect on pathogenic bacteria of wheat scab and wheat stem rot, has a remarkable prevention and treatment effect on the wheat stripe rust, the wheat stem rot and the wheat scab, has a better prevention effect than a treatment effect, and can be widely applied to prevention and treatment of wheat stripe rust, wheat stem rot and wheat scab. Meanwhile, the strain has no adverse effect on growth and development of wheat and can be applied to prevention and treatment of wheat stripe rust, basal stem rot and gibberellic disease. The invention provides a new strain resource for a biocontrol resource library of plant diseases.

The invention belongs to the technical field of biological control, and particularly relates to the technical field of crop stem rot control. The invention provides a bacillus velezensis Gc8, and the preservation number of the bacillus velezensis Gc8 is CGMCC (China General Microbiological Culture Collection Center) No. 28991. The bacillus velezensis Gc8 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 14, 2023, the preservation number of the bacillus velezensis Gc8 is CGMCC No.28991, the center is called CGMCC for short, and the address of the center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67, kitasapora fluorescens pf27, kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27. The compound microbial agent can inhibit spore germination of pathogenic microorganisms, and has the effect of promoting growth of corn while resisting diseases.

The invention relates to the technical field of biological prevention and control, in particular to a trichoderma subatroviride Thp30 strain and application thereof. The trichoderma subatroviride Thp30 is preserved in Guangdong Microbial Culture Collection Center on May 22, 2025, and the preservation number is GDMCC No: 66381. The trichoderma pseudoviride Thp30 provided by the invention can inhibit the growth of wheat stem rot pathogen fusarium graminearum, apple anthracnose pathogen colletotrichum gloeosporioides, botrytis cinerea pathogen botrytis cinerea, wax gourd fusarium wilt pathogen fusarium oxysporum and pear anthracnose pathogen colletotrichum gloeosporioides, and promote the growth of wheat, soybean and wax gourd; the wheat basal stem rot can be effectively prevented and treated, and the freezing resistance of wheat can be enhanced.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a method for identifying a compound for inhibiting synthesis of vomitoxin from wheat stem rot pathogen. The method comprises the following steps: cloning an open expression cassette of a key catalytic enzyme gene FpgTri4 in a process of biologically synthesizing vomitoxin by pathogenic bacteria, constructing recombinant expression plasmids from the open expression cassette, and transduction the recombinant expression plasmids into a Fusarium pseudograminearum strain to induce expression to generate toxosome; and the inhibitor with inhibition on the generation of vomitoxin can inhibit the generation of toxosome in the recombinant strain, so that the related inhibitor can be screened by using the method. The method disclosed by the invention is rapid, simple and easy to implement, can be used for high-throughput screening, explores compounds capable of preventing diseases and controlling toxicity, and has important significance on scientific prevention and treatment of wheat stem rot.

The invention discloses a multifunctional talaromyces sp. Fungus RB-2 for preventing and controlling ginger stem rot and application of the multifunctional talaromyces sp. Fungus RB-2, and belongs to the technical field of microorganisms. The talaromyces sp. Fungus RB-2 is preserved in the China Center for Type Culture Collection, the preservation number of the talaromyces sp. Fungus RB-2 is CCTCC NO: M 20251722, and the preservation date of the talaromyces sp. Fungus RB-2 is July 28, 2025. The talaromyces sp. Strain RB-2 with the effect of inhibiting the growth of pathogenic bacteria is separated and purified, and the strain RB-2 has remarkable antagonistic activity on ginger stem rot pathogenic bacterium group pythium aphanidermatum and various economic crop pathogenic bacteria and has the potential of being developed into a biocontrol inoculant. The talaromyces sp. Strain RB-2 also has phosphorus dissolving and growth promoting capabilities, can remarkably promote the growth of ginger, and has the potential of preparing a soil conditioner and a microbial fertilizer, so that the micro-ecology of soil is improved, and the productivity of crops is enhanced.

The invention relates to the technical field of genetic engineering, and discloses a fusarium pseudograminearum effect protein FpE01 and application thereof in wheat disease resistance. The amino acid sequence of the Fusarium pseudograminearum effect protein FpE01 is as shown in SEQ ID NO. 1. The nucleotide sequence of the coding gene of the Fusarium pseudograminearum effect protein FpE01 is as shown in SEQ ID NO. 2. The fusarium pseudograminearum effect protein FpE01 and the application thereof in wheat disease resistance are adopted, a knockout mutant of the coding gene FpE01 is constructed, the toxic function of the coding gene FpE01 in the process that wheat is infected by fusarium pseudograminearum is determined, then the effect protein is utilized to construct a wheat strain resistant to stem rot, and the wheat strain resistant to stem rot is obtained. And an excellent wheat material is provided for cultivation of basal stem rot resistant varieties.

The invention relates to the technical field of gene engineering, and particularly discloses application of a pathogenic gene FpMetR for controlling stem rot pathogenic bacteria Fusarium pseudograminearum, specifically, the colony morphology of a deletion mutant strain of the FpMetR gene is changed, the sporulation quantity is reduced, the spore germination rate is reduced, and the pathogenicity of the mutant strain is completely lost, the DNA sequence of the FpMetR gene is as shown in SEQ ID No: 1, and the DNA sequence of the FpMetR gene is as shown in SEQ ID No: 2. According to the invention, the pathogenic mechanism is clarified: a scientific basis is provided for deeply understanding the pathogenic mechanism of pathogenic bacteria by revealing the key effect of the FpMetR gene in the pathogenic process of the fusarium pseudograminearum, and a new thought and a target gene are provided for the field of plant anti-fusarium pseudograminearum genetic engineering by virtue of the effect of the FpMetR gene in the aspects of regulating and controlling the growth and pathogenicity of wheat. By applying the gene or a regulation mechanism thereof to genetic improvement of crops such as wheat, new varieties with disease resistance are expected to be cultivated, dependence on chemical pesticides is reduced, and environmental friendliness is improved.

The invention discloses a bactericidal composition containing WML-01 and a triazole compound, and is characterized in that the bactericidal composition is composed of the WML-01 and the triazole compound, and the mass ratio of the WML-01 to the triazole compound is (10: 1)-(1: 10). The bactericidal composition taking the WML-01 and the triazole medicament as effective components has a synergistic effect on prevention and treatment of rice bakanae disease, wheat stem rot, wheat scab, plant root rot and sclerotinia rot of colza, can effectively improve the disease prevention and treatment effect, reduces the pesticide consumption, delays the occurrence of drug resistance, improves the crop yield, and has a good application prospect. The method is safe, effective and economical, and has a wide market application prospect.

The invention discloses a molecular marker of a wheat stem rot disease-resistant site Qfcr.cau-1B and application of the molecular marker, and belongs to the technical field of molecular markers and plant genetic breeding. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1, and T / A mutation exists at the 201st basic group of the sequence as shown in SEQ ID NO.1. A pair of STARP primer groups is designed for the molecular marker, and the primer groups can accurately detect the molecular marker. The molecular marker can be used for identifying the resistance of wheat to stem rot or screening varieties or strains which are resistant to and susceptible to wheat stem rot. The molecular marker provided by the invention can rapidly and accurately identify the resistance of wheat to FCR, and provides a very valuable molecular tool for screening wheat anti-FCR germplasm resources.

The invention belongs to the technical field of biological prevention and control of plant diseases, and provides bacillus subtilis and application thereof in prevention and control of corn diseases. The preservation number of the bacillus subtilis SEB-34 is CGMCC (China General Microbiological Culture Collection Center) No. 26419. The invention also provides a microbial agent produced by using the SEB-34 strain, and the microbial agent can be used for preventing and treating plant diseases caused by fusarium graminearum or pelamys maydis. The bacillus subtilis provided by the invention is applied through seed treatment before sowing or root drip irrigation after seedling emergence, has the effects of preventing diseases, promoting growth and increasing yield, and can effectively reduce the occurrence of corn southern leaf blight and basal stem rot. The microbial agent provided by the invention is in a semi-dormant state, has relatively strong stress resistance, is more stable to store, ensures the effect, and effectively reduces the production and transportation cost at the same time. The invention also provides beneficial microbial resources for green prevention and control of corn diseases, and has good application prospect and high application value.

The invention discloses application of a TpGST gene in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. According to the application of the TpGST gene disclosed by the invention in regulating and controlling the ear rot and stem rot resistance of maize fusarium graminearum, the nucleotide sequence of the TpGST gene is shown as SEQ ID NO. 1. Fusarium graminearum is inoculated to ears and stems of an overexpressed TpGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic line is improved; fusarium graminearum is inoculated to ears and stems of the corn hybrid agricultural large 3138 overexpression TpGST transgenic line, and the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic hybrid is improved.

The invention relates to talaromyces cordiformis and application thereof. The talaromyces sp. Strain Dz-1118 is found to have an antagonistic inhibition effect on fusarium solani, fusarium oxysporum, fusarium pseudograminearum, fusarium verticillium, fusarium graminearum or botrytis cinerea, so that the talaromyces sp. Strain Dz-1118 can be used for preventing and treating root rot, fusarium wilt, stem rot, spike rot, gibberellic disease or gray mold.

The invention relates to a wheat stem rot regulatory gene Tatrx-m, and an encoding protein, a recombinant vector and an application of the wheat stem rot regulatory gene Tatrx-m. The genomic sequence of the gene is SEQ ID NO.1, the CDS sequence of the gene is SEQ ID NO.2, and thioredoxin shown as SEQ ID NO.3 is encoded. The invention further provides a specific primer pair used for gene identification, a silent vector (Tatrx-m-VIGS) and an overexpression vector (LGY-OE3-Tatrx-m) are constructed, and the function of the primer pair is verified through a virus-induced gene silencing (VIGS) technology and agrobacterium-mediated transformation of wheat. Experiments show that silence of Tatrx-m results in significant reduction of wheat stem rot resistance, and overexpression of the gene can improve resistance. Based on hypha quantification, DAB staining, H2O2 content and cell death analysis, Tatrx-m is revealed to enhance wheat resistance by regulating active oxygen removal and cell wall strengthening pathways. The gene TaTrx-m has the positive regulation effect in wheat stem rot for the first time, can be applied to disease-resistant molecular marker development, gene editing and transgenic breeding, provides core genetic resources for disease-resistant variety breeding, and has theoretical and application values.

The invention relates to application of tryptophol in prevention and treatment of corn bacterial stem rot and relates to the technical field of biological prevention and control, the pathogenic bacterium of the corn bacterial stem rot is Dickeya zeae, it is found for the first time that tryptophol can inhibit the mobility of the Dickeya zeae, reduce the activity of cell wall degrading enzyme and relieve the disease degree, and the tryptophol can inhibit the activity of the Dickeya zeae, so that the tryptophol can be used for preventing and treating the corn bacterial stem rot. The tryptophol can effectively reduce the pathogenicity of tryptophol, but has no adverse effect on the cell viability and form of the corn dibasic bacteria, which indicates that the effect of tryptophol on the corn dibasic bacteria is realized by inhibiting the pathogenicity of bacteria instead of killing bacteria, so that tryptophol acts on the corn bacterial stem rot, the drug resistance is not easy to generate, and the tryptophol can be used for treating the corn bacterial stem rot. The tryptophol is expected to become a substitute of antibiotics and is used for preventing and treating the corn bacterial stem rot, the diseases can be effectively controlled, the selective pressure of drug resistance development can be relieved, and a new solution is provided for sustainable prevention and treatment of the corn bacterial stem rot.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

The invention provides a Brevibacillus formmosus strain SXM-17, a derivative product of the Brevibacillus formmosus strain SXM-17 and application of the Brevibacillus formmosus strain SXM-17 to prevention and control of crop diseases, and belongs to the technical field of functional microorganisms. The strain SXM-17 is separated and purified from wheat plants, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.30198, and the strain SXM-17 has a good inhibition effect on gibberellic disease bacteria, physalospora piricola, botrytis cinerea, alternaria solani, stem rot bacteria, gaeumannosis and the like. Experiments show that a culture solution, a bacterial suspension and a supernatant of the strain SXM-17 have good prevention and treatment effects. In addition, the strain SXM-17 also has a growth promoting effect on crops planted in a natural planting environment and a salinization environment. The strain SXM-17 provided by the invention enriches a gibberellic disease biocontrol strain resource library and green and efficient biological control microbial resources.

The invention relates to the field of microorganisms, in particular to streptomyces noursei S83, a biological agent and application of the streptomyces noursei S83. Aiming at the occurrence of common tobacco diseases, a strain of streptomyces noursei S83 capable of effectively preventing and treating the tobacco target leaf spot is separated and screened, and the preservation number of the streptomyces noursei S83 is CGMCC No.31252. The application performance of the compound is evaluated in specific embodiments, it is found that the compound has prevention and treatment effects on tobacco target leaf spot, rice sheath blight disease, rice blast, wheat stem rot and other diseases, and the prevention effect on the tobacco target leaf spot reaches 52.8%; meanwhile, the growth of the tobacco can be promoted. Therefore, the streptomyces noursei S83 provided by the invention is a multifunctional strain, and provides powerful support for green prevention and control of plant diseases such as tobacco and the like.

The present invention discloses a kit for detecting Fusarium pseudo-verticillium based on RPA-CRISPR / Cas system and its application, wherein the Cas protein is Cas12, the kit includes RPA primers and crRNA, the Fusarium includes Fusarium pseudo-verticillium, and the RPA primers and crRNA of Fusarium pseudo-verticillium are primer combinations FverF1 / FverR1 and Fv-crRNA, respectively. The present invention develops a field visualization rapid detection technology for corn stalk rot pathogens based on recombinase polymerase amplification technology combined with CRISPR / Cas12a for the widely distributed, highly isolated and highly pathogenic corn stalk rot pathogens. The technology can react at a constant temperature of 37-42°C without the need for complex instruments and equipment, and CRISPR / Cas12a can recognize and cut specific nucleic acid sequences, thereby further increasing the specificity and sensitivity of the detection on the basis of RPA.

The invention belongs to the technical field of plant biology, and particularly relates to wheat stem rot related protein TaNDR1 and application thereof. According to the invention, a gene with a non-microspecies specific disease resistance function is cloned from wheat and is named as TaNDR1. According to the invention, a barley stripe mosaic virus induced vector for silencing the TaNDR1 gene in wheat is constructed, the expression of the TaNDR1 gene is reduced, a silent TaNDR1 wheat plant is obtained, and after the silent TaNDR1 wheat is inoculated with stem rot pathogen, the stem rot resistance of the wheat is obviously reduced. Therefore, after the expression quantity of the TaNDR1 in the susceptible wheat plant is increased, the stem rot resistance of the susceptible wheat plant can be obviously improved, so that the gene can be used for cultivating the stem rot resistant wheat.