351 results about "Stem rot" patented technology

The present invention provides novel microorganisms, compositions and methods of use thereof, for treating, inhibiting or preventing the developing of a plant pathogenic disease and for killing or inhibiting growth of a variety of pests or pathogens. Provided are compositions comprising a novel endophytic fungal organism effective to inhibit the growth of or kill pests and pathogenic microbes, including Ganoderma boninense. Invention compositions are especially useful in preventing and treating basal stem rot in the oil palm, and can be applied on or in the vicinity of the plant or used to sterilize the plant growth medium prior to or concurrent with plant growth therein. The disclosure further provides substantially purified polynucleotides and polypeptides encoded thereby, together with methods of using those products, for example for making transgenic organism.

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

The present invention provides compositions and methods for treating, inhibiting or preventing the developing of a plant pathogenic disease. The compositions comprise volatile organic compounds effective to inhibit the growth of, or kill pathogenic microbes, including Ganoderma boninense. Invention compositions are especially useful in preventing and treating basal stem rot in the oil palm, and can be applied in the vicinity of the plant or used to sterilize the plant growth medium prior to or concurrent with plant growth therein.

The invention provides carnation high-efficiency rootone which is characterized by containing the following materials according to weight part: 10 parts of rhodofix, 1-2 parts of seradix, 80-120 parts of mildothane, 15-30 parts of streptomycin and 6000-6400 parts of talcum powder. In the invention, aiming at the problems of root rot and stem rot of the carnation easily harmed by epiphyte and bacteria and being combined with the characteristics that roots produced by rhodofix NAA induction are less in quantity and thick and adventitious roots produced by seradix IBA action are thin and long, NAA and IBA are mixed in the rootone, and meanwhile, mildothane used for sterilizing and agricultural streptomycin are added; therefore, the rooting ratio of the carnation is high, the growing roots are long and thick, the antibiosis and disease-resisting capacity is enhanced, and the root rot ratio is greatly reduced, and the invention has the advantages of tidy rooting, high field production survival rate, convenient usage, low cost, good effect, and the like and is very convenient for the growth of the carnations.

The invention discloses a high-phosphorus high-potassium controlled-release fertilizer for peanuts and a preparation method of the high-phosphorus high-potassium controlled-release fertilizer, belonging to the technical field of fertilizers. The high-phosphorus high-potassium controlled-release fertilizer comprises the following raw materials in parts by weight: 20-30 parts of urea, 60-80 parts of diammonium phosphate, 30-40 parts of potassium chloride, 8-10 parts of magnesium ammonium phosphate, 8-10 parts of potassium feldspar, 1-2 parts of boric acid, 1-2 parts of ferrous sulfate, 1-2 parts of manganese sulfate, 1-2 parts of zinc sulfate, 1-2 parts of sodium molybdate, 5-10 parts of traditional Chinese medicine additive, 8-10 parts of gypsum powder and 20-30 parts of urea formaldehyde. When being used, the traditional Chinese medicine additive is decocted to form a traditional Chinese medicine extracting solution by adding water; the urea formaldehyde containing Chinese herb extracts serves as an envelope, and the gypsum powder is adhered outside the envelope; and the other raw materials are wrapped in the envelope. After being fertilized for one time, the high-phosphorus high-potassium controlled-release fertilizer meets the requirements of crops on nutrients such as nitrogen, phosphorus, potassium, calcium and sulfur in the entire growth period. Meanwhile, the high-phosphorus high-potassium controlled-release fertilizer can be used for increasing the setting ratio and the yield by above 15% and meanwhile greatly reducing the occurrence rate of stem rots, root rots and the like of peanuts.

The invention relates to a Trichoderma strain for antagonizing soil-borne disease, belonging to the field of biological control. The Trichoderma strain is Trichoderma asperellum ZJSX5003 CGMCC No.6480. The Trichoderma strain provided by the invention has the characteristics of high growth speed and high spore production amount; the in-vitro standoff pathogen inhibition rates are respectively up to 79.79%, 73.68% and 72.25%, the pathogen spore survival rates are respectively 0.00%, the in-vivo preventive effects for cucumber fusarium wilt and maize stem rot are respectively 85.08% and 66.67%, the enzyme activity of chitinase is 4.0783U, the enzyme activity of beta-1,3-glucanase is 0.8050U, the enzyme activity of extracellular proteinase is 4.8503U, and the content of silicone, terpene and carboxylic acid having antagonistic actions in antibiotic secondary metabolites is 44.44%. The Trichoderma strain provided by the invention has the advantages of high antagonism and high specificity. The biological pesticide produced from the strain can effectively control the soil-borne disease, thereby ensuring the stable increase of crop and vegetable yields.

The invention discloses a high-yield planting method for preventing and treating corn stem rot in semi-arid regions, belonging to the technical field of agricultural planting and culturing. The high-yield planting method comprises the following steps: land selection and preparation, seed selection, seed treatment, ridging, base fertilizer application, seeding, drop irrigation, water feeding, weeding, membrane lamination, top application, field management, corn stem rot prevention, harvesting and the like. According to the high-yield planting method, the problem that the seeding emergence rate of corns in the second year is influenced by the total straw application of straws is solved; by processing corn seeds, the emergence rate can be improved by more than or equal to 5%, and the straw turnover in the second year is not influenced; the preventing and treating effect of the corn stem rot reaches more than or equal to 70%; water is saved by more than or equal to 40%, and the corn yield is increased to be 800-1000 kilograms per hectare; adverse factors caused by the ecological environment to the agricultural production are overcome, the traditional agricultural production mode is changed, the unit yield is increased, the yield and the efficiency are increased, and the effects of reducing wind erosion of soil, protecting the environment and improving the drought resisting capacity and the production stability of crops are achieved.

The invention discloses a method for inoculating fresh corn indoors by using pathogenic fusarium verticillioides. The method disclosed by the invention comprises the steps of separating to obtain bacterial stains of the pathogenic fusarium verticillioides; carrying out expanding propagation of the fusarium verticillioides; preparing a corn variety to be inoculated; carrying out co-culture inoculation on the fusarium verticillioides and corn seeds; seeding; carrying out evaluation of disease resistance of a corn plant. Without depending on natural conditions of land for growing field crops, the method disclosed by the invention is few in inoculated bacteria, little in environmental influence, better in effect and higher in controllability, can be combined into field inoculation tests, and verifies resistance of the variety on corn stalk rot in an auxiliary mode. The method disclosed by the invention provides an effective corn inoculation technology for fusarium verticillioides, and can solve the early evaluation problem of corn for stalk rot resistance.

The invention relates to a panax notoginseng seedling protection film, and a preparation method and an application thereof. The film provided by the invention is a water-soluble gel prepared by using a water-soluble film-forming material, panax notoginseng polysaccharide, a root growth promoter, a preservative, and a pesticide. With the material, a protection film can be formed on the surface of a panax notoginseng seedling. With the panax notoginseng seedling protection film provided by the invention, water loss of panax notoginseng seedling during a transplanting process can be reduced, and seedling surface skin damage caused by abrasion during transportation or during transplanting can be reduced. Therefore, fungal infection or insect attack caused by damages after transplanting can be reduced, occurrence rate of panax notoginseng root rot, sclerotinia stem rot, sheath blight, anthracnose, and blight can be reduced, and panax notoginseng survival rate can be improved. The panax notoginseng seedling protection film provided by the invention has good physiochemical performances such as film-forming performance, water resistance, air permeability, water permeability, and the like. Therefore, healthy growth of crops can be protected.

The invention provides a primer combination for detecting succulent plant stem fusarium moniliforme based on LAMP (Loop-mediated Isothermal Amplification) and application thereof. The LAMP primer combination for detecting the succulent plant stem fusarium moniliforme consists of 1 pair of outside primers F3/B3 and a pair of inside primer FIP/BIP, wherein the F3/B3 and FIP/BIP primer sequences areshown as SEQ ID NO. 1 to 4. By adopting the detection system disclosed by the invention, the succulent plant stem fusarium moniliforme can be detected rapidly, conveniently and efficiently at high specificity and high sensitively through incubation at the constant temperature of 65 DEG C for 1 hour without complex and expensive instruments, and in-situ detection of the succulent plant stem fusarium moniliforme can be met well. The detection system can be widely applied to early diagnosis of field diseases of establishment units as well as monitoring and identification of viruses, and a reliable technological and theoretical basis is provided for prevention and control of the succulent plant stem fusarium moniliforme.

Disclosed is the agricultural pesticide containing camptothecine or camptothecine derivatives and the use thereof, wherein the pesticide comprises camptothecine or its derivatives 0.02-2.0%, emulsifying agent 1.0-20%,penetrating agent 0.5-10%, and balancing organic solvent. The pesticide can be used for controlling agronomic crop downy mildew, leaf streak, pythium blight, head blight, root and stem rot, rhizoctonia rot, damping-off, wilt,bitter rot, powdery mildew, black shank, brown spot, leaf scab, ring rot, black pox, gray mold,phytophthora rot, ulcer disease, black spot, bacterial wilt, angular leaf spot, leaf streak and leaf scar.

The invention relates to an inoculation and identification method for crown rot caused by fusarium graminearum of wheat at seedling stage, which pertains to the technical field of plant protection and is used for fast identifying and screening wheat germplasm resources with resistance to the crown rot caused by fusarium graminearum from wheat resources in China. After activation, fusarium graminearum is inoculated in a liquid nutrient medium of 0.6 percent of green bean soup which is sterilized with high pressure at a temperature of 121 DEG C, and cultured in a shaker for 72h under a temperature of 25 DEG C, and then fusarium graminearum suspension of 10 <5> spores/ml is obtained; wheat seeds are sprouted after disinfection and are sowed in sterilized earth after germination; inoculation is started 10 days after seedling emergence; the crown rot inspection of wheat stem base is carried out 35 days after inoculation. The inoculation and identification method has the advantages of setting up the inoculation and identification method for crown rot caused by fusarium graminearum of wheat at seedling stage, providing a basis for the research of the crown rot caused by fusarium graminearum, being capable of fast screening wheat resistance resources and improving the utilization of the wheat resources.

The invention discloses a method for grafting cultivation of a pitaya seedling, belonging to the technical field of cultivation of fruit seedlings. The method comprises the following steps: selection and cultivation of a rootstock, selection of scions, grafting, management after grafting, etc. The pitaya seedling cultivated by using the method provided by the invention has strong adaptability and stress resistance, especially has strong capability of resisting root-rot and stem-rot diseases, can maintain the characteristics of an original variety, and has the advantages of high seedling survival rate, rapid germination rate and strong growth vigor.

The invention discloses a high-yield and high-quality planting method of Paris polyphylla, which includes steps of land selection and preparation, seed selection and treatment, sowing, transplanting, field management, harvesting and others. The method uses harmless fertilizer during the Paris polyphylla planting process, so as to avoid pollutions of the Paris polyphylla products, local soil, water source and air by chemical factors; plant ash is sprayed to prevent and treat stem rot; and plant ash lixivium is sprayed to prevent and treat aphid and red spider, thereby reaching the ecological and green production; the Paris polyphylla product has the advantages of being high in drug effect, good in quality, green and environmental-friendly, low in planting method, high in benefit, simple in operation, easy to popularize, high in earnings, and low in risk; the method provides a new optimal scheme for the artificial scaled planting of the Paris polyphylla; therefore, the method is good in both economic benefit and social benefit.

The present invention provides one new gene MgKIN17 from Pyricularia gisea, and the gene features that it has the amino acid sequence from the site 1 to the site 3544 in the SEQ ID No. 1, the cDNA with the nucleotide sequence of SEQ ID No. 2, the promoter with the nucleotide sequence of SEQ ID No. 4, and coded protein with the amino acid sequence of SEQ ID No. 3. The insertion mutation and gene complementation of the present invention shows that the destruction of the gene leads the formation of Pyricularia gisea infecting organ and reduced lesion capacity of rice. Wheat bakanae fungus, corn stalk rot and other pathogenic fungus have homogenous protein with homogeneity over 40 % to MgKIN17 and maybe similar biological function. The gene of the present invention and its protein expression and modification may be used as the target site for designing and screening antifungal medicines.

The invention relates to a microorganism bacterium agent for preventing and treating the ginger basal stem rot and a preparation method and application thereof. The microorganism bacterium agent comprises trichoderma longibrachiatum leavening and a mixed material; the mixed material is formed by humic acid, amino acid and medical stones; a mass ratio of the trichoderma longibrachiatum leavening to the humic acid to the amino acid to the medical stones is (3 to 6):(1 to 2):(1 to 2):(4 to 5); the number of active living bacteria in the trichoderma longibrachiatum leavening is greater than or equal to 2*10<9> per gram. According to the invention, the trichoderma longibrachiatum leavening is applied to prevention and treatment of the ginger basal stem rot; the microorganism bacterium agent for preventing and treating the ginger basal stem rot is prepared; the obtained number of the active living bacteria in trichoderma longibrachiatum is not smaller than 2*10<9> per gram; a prevention and treatment rate for the basal stem rot is high; the morbidity of the ginger basal stem rot can be greatly reduced by not less than 80%; yield of ginger can be improved by not less than 10%; the economic benefits are obvious; quality of the ginger product is improved.

The invention provides a seed coating agent of waxy corn, which comprises the following components in percentage by weight: 1 to 2 percent of polyvinyl alcohol, 3 to 8 percent of gum arabic, 0.1 to 1percent of surfactant, 2 to 5 percent of antifreezing agent, 0.1 to 1 percent of defoaming agent, 1 to 2 percent of warning color, 0.5 to 1 percent of thiamethoxam, 0.5 to 2 percent of cypermethrin, 1to 2 percent of carboxin, 0.1 to 1 percent of fludioxonil, 0.1 to 2 percent of chitosan, 1 to 2 percent of potassium fertilizer, and the balance of water. The seed coating agent achieves effective control efficiency to soil insects (cutworms, grubs and the like), stem rot, head smut and aphids, generates obvious control efficiency to diseases and insect damages, and has low dosage. The germination speed of waxy corn seeds is increased by 0.5 day, seedlings grow robustly, the dry fresh weight is increased, and the comprehensive control efficiency of plant diseases and insect pests reaches 90.80 percent; and at the harvest time, the spike length, the spike thickness and the spike grain number are improved compared with a contrast, and the yield of the corn is improved by 10 to 15 percent.

The invention relates to a pyraclostrobin, ningnanmycin and imidacloprid-containing flowable concentrate for seed coating and a preparation method thereof. The flowable concentrate for seed coating comprises an active ingredient and auxiliary ingredients, wherein the active ingredient is formed by compounding pyraclostrobin, ningnanmycin and imidacloprid; the mass ratio of the pyraclostrobin to the ningnanmycin to the imidacloprid is (1-80): (1-80): (1-80). According to the flowable concentrate for seed coating, the pyraclostrobin, the ningnanmycin and the imidacloprid are compounded according to a certain proportion; as the pyraclostrobin, the ningnanmycin and the imidacloprid have systemicity, the flowable concentrate for seed coating has good compatibility, is capable of killing diseases and pests, has good pesticide effects for the underground and overground germs and pests, and can be used for effectively preventing and treating the plant diseases such soybean root rot disease and rice stem rot disease and the pests such as aphids, thrips and trialeurodes vaporariorum at the seedling stage of the crops; moreover, the flowable concentrate for seed coating is capable of inducing the plants to generate resistance and disease tolerance for the intrusive viruses so as to well solve the problems in practical production and save the time and labor; compared with the other drug delivery technologies, the flowable concentrate for seed coating is relatively less in pollution.

The invention discloses clone and functional analysis of corn anti-fusarium graminearum stem rot gene ZmAuxRP1. The invention provides a protein. The protein is following a) or b): a) a protein consisting of amino acid sequences shown in sequence 2 in a sequence table; b) a protein derived from (a) substituting and/or deleting and/or adding one or more amino acid residual groups to the amino acid sequence shown in the sequence 2 in the sequence table and having the same function as (a). The new gene ZmAuxRP1 is discovered, transgenic corn is obtained by interfering the ZmZuxRP1 gene expression in wild corn, and compared with the non-interfered wild ZmAuxRP1, the corn stem rot resistance can be improved; by virtue of over-expression experiment, the interfered ZmAuxRP1 gene expression can be used for the paddy rice to resist the corn stem rot disease.