229 results about "Stem rot" patented technology

The invention relates to the field of fertilizers, in particular to a stable fertilizer for preventing and treating peanut root rot and a preparation method thereof, and the fertilizer comprises a fertilizer inner core layer, a nano microbial metabolite layer and a microbial protective layer from inside to outside. According to the stable fertilizer for preventing and treating peanut rhizome rot, the capsule embedding inhibitor made of humic acid and the trichoderma aureoviride metabolite are adopted to construct a dual nitrification inhibition system, and the capsule embedding inhibitor and the trichoderma aureoviride metabolite have a synergistic effect, so that the nitrification effect is reduced, the nitrogen fertilizer utilization rate is increased, and non-point source pollution is reduced.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus velezensis and application thereof. The bacillus velezensis strain is separated and screened from rhizosphere soil of wheat in a main wheat area in Hebei Province, the growth characteristics and physiological and biochemical characteristics of the bacterial colony are identified, then 16S rDNA sequencing is carried out, Blast homology comparison is carried out in a GenBank database, the bacterial strain is identified to be bacillus velezensis through the analysis, and the bacillus velezensis strain is identified to be bacillus velezensis. The fungicide containing the bacillus velezensis has a good prevention and treatment effect on wheat stem rot through seed coating.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention discloses bacillus velezensis and application of the bacillus velezensis in prevention and treatment of basal stem rot, the classification name of the bacillus velezensis is bacillus velezensis GN-22, the bacillus velezensis is preserved in China Center for Type Culture Collection (CCTCC), the preservation number of the bacillus velezensis is CCTCC NO: M2025566, and the preservation date is March 24, 2025. The bacillus velezensis GN-22 disclosed by the invention belongs to rhizosphere growth-promoting bacteria, has a remarkable inhibition effect on different pathogenic fungi such as fusarium graminearum, fusarium pseudograminearum, fusarium asiaticum, fusarium verticillioides and fusarium oxysporum, and plays an important role in biological control of diseases such as corn and wheat stem rot; meanwhile, the strain has a growth promoting effect and can promote corn growth. The strain provided by the invention has the advantages of simple culture conditions, easiness in preservation, easiness in industrial production and good development and application prospects.

The present invention relates to the technical field of molecular markers for maize resistance to stalk rot and genetic breeding, and in particular, to a QTL related to maize resistance to stalk rot, SNP molecular markers closely linked with the QTL, and a use of the SNP molecular markers. Provided is a QTL related to maize resistance to stalk rot, wherein the QTL is located on maize chromosome 10. Also provided are SNP molecular markers Ks4 and Ks6 closely linked with a QTL site for maize resistance to stalk rot. By detecting the SNP molecular markers, the genotype of a maize material under test can be accurately identified in a high-throughput manner, a material resistant to stalk rot is selected, and phenotype identification and assisted screening of the maize resistance to stalk rot can also be carried out in a seedling stage, thus significantly reducing production cost and significantly enhancing the efficiency of breeding maize resistant to stalk rot.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention provides application of a pseudomonas parabarium YC15 strain in efficient prevention and treatment of wheat stem rot and various pathogenic bacteria. The invention provides a new application of the pseudomonas parabarium YC15 strain in prevention and control of plant diseases, and research shows that the YC15 strain has broad-spectrum bacteriostatic activity, has remarkable inhibitory activity on various plant pathogenic bacteria, can inhibit pathogenic fungi such as wheat stem rot, sesame root rot and colletotrichum gloeosporioides, and has a wide application prospect in prevention and control of plant diseases. And the compound has a relatively good inhibition effect on pathogenic bacteria, namely sesames pseudomonas solanacearum. The YC15 strain is applied to prevention and control of wheat stem rot, and pot experiments show that after the YC15 strain is adopted for treatment, plant lesions can be reduced, the inhibition effect of pathogenic bacteria on plants can be broken, the pathogenic bacteria can be effectively inhibited, plant growth can be promoted, and the wheat stem rot can be efficiently prevented and controlled. The invention provides new application of the YC15 strain, and the YC15 strain can be used for preventing and treating various plant protobacteria and diseases thereof, and has good development and application potential as a biocontrol bacterium.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to Lecanicillium lecanii as well as a fungicide and application thereof, Lecanicillium lecanii GC-2 is obtained by screening and separating phyllospheric microorganisms, and the strain is proved to have relatively strong inhibitory activity on wheat stripe rust, fusarium graminearum and pseudocereal fusarium; a pot experiment verifies that the strain has a very strong inhibition effect on uredospores of wheat stripe rust, has a relatively strong antagonism effect on pathogenic bacteria of wheat scab and wheat stem rot, has a remarkable prevention and treatment effect on the wheat stripe rust, the wheat stem rot and the wheat scab, has a better prevention effect than a treatment effect, and can be widely applied to prevention and treatment of wheat stripe rust, wheat stem rot and wheat scab. Meanwhile, the strain has no adverse effect on growth and development of wheat and can be applied to prevention and treatment of wheat stripe rust, basal stem rot and gibberellic disease. The invention provides a new strain resource for a biocontrol resource library of plant diseases.

The invention belongs to the technical field of biological control, and particularly relates to the technical field of crop stem rot control. The invention provides a bacillus velezensis Gc8, and the preservation number of the bacillus velezensis Gc8 is CGMCC (China General Microbiological Culture Collection Center) No. 28991. The bacillus velezensis Gc8 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 14, 2023, the preservation number of the bacillus velezensis Gc8 is CGMCC No.28991, the center is called CGMCC for short, and the address of the center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67, kitasapora fluorescens pf27, kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27. The compound microbial agent can inhibit spore germination of pathogenic microorganisms, and has the effect of promoting growth of corn while resisting diseases.

The invention relates to the technical field of biological prevention and control, in particular to a trichoderma subatroviride Thp30 strain and application thereof. The trichoderma subatroviride Thp30 is preserved in Guangdong Microbial Culture Collection Center on May 22, 2025, and the preservation number is GDMCC No: 66381. The trichoderma pseudoviride Thp30 provided by the invention can inhibit the growth of wheat stem rot pathogen fusarium graminearum, apple anthracnose pathogen colletotrichum gloeosporioides, botrytis cinerea pathogen botrytis cinerea, wax gourd fusarium wilt pathogen fusarium oxysporum and pear anthracnose pathogen colletotrichum gloeosporioides, and promote the growth of wheat, soybean and wax gourd; the wheat basal stem rot can be effectively prevented and treated, and the freezing resistance of wheat can be enhanced.

The invention discloses a multifunctional talaromyces sp. Fungus RB-2 for preventing and controlling ginger stem rot and application of the multifunctional talaromyces sp. Fungus RB-2, and belongs to the technical field of microorganisms. The talaromyces sp. Fungus RB-2 is preserved in the China Center for Type Culture Collection, the preservation number of the talaromyces sp. Fungus RB-2 is CCTCC NO: M 20251722, and the preservation date of the talaromyces sp. Fungus RB-2 is July 28, 2025. The talaromyces sp. Strain RB-2 with the effect of inhibiting the growth of pathogenic bacteria is separated and purified, and the strain RB-2 has remarkable antagonistic activity on ginger stem rot pathogenic bacterium group pythium aphanidermatum and various economic crop pathogenic bacteria and has the potential of being developed into a biocontrol inoculant. The talaromyces sp. Strain RB-2 also has phosphorus dissolving and growth promoting capabilities, can remarkably promote the growth of ginger, and has the potential of preparing a soil conditioner and a microbial fertilizer, so that the micro-ecology of soil is improved, and the productivity of crops is enhanced.

The invention discloses a bactericidal composition containing WML-01 and a triazole compound, and is characterized in that the bactericidal composition is composed of the WML-01 and the triazole compound, and the mass ratio of the WML-01 to the triazole compound is (10: 1)-(1: 10). The bactericidal composition taking the WML-01 and the triazole medicament as effective components has a synergistic effect on prevention and treatment of rice bakanae disease, wheat stem rot, wheat scab, plant root rot and sclerotinia rot of colza, can effectively improve the disease prevention and treatment effect, reduces the pesticide consumption, delays the occurrence of drug resistance, improves the crop yield, and has a good application prospect. The method is safe, effective and economical, and has a wide market application prospect.

The invention discloses application of a TpGST gene in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. According to the application of the TpGST gene disclosed by the invention in regulating and controlling the ear rot and stem rot resistance of maize fusarium graminearum, the nucleotide sequence of the TpGST gene is shown as SEQ ID NO. 1. Fusarium graminearum is inoculated to ears and stems of an overexpressed TpGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic line is improved; fusarium graminearum is inoculated to ears and stems of the corn hybrid agricultural large 3138 overexpression TpGST transgenic line, and the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic hybrid is improved.

The invention relates to talaromyces cordiformis and application thereof. The talaromyces sp. Strain Dz-1118 is found to have an antagonistic inhibition effect on fusarium solani, fusarium oxysporum, fusarium pseudograminearum, fusarium verticillium, fusarium graminearum or botrytis cinerea, so that the talaromyces sp. Strain Dz-1118 can be used for preventing and treating root rot, fusarium wilt, stem rot, spike rot, gibberellic disease or gray mold.

The invention relates to a wheat stem rot regulatory gene Tatrx-m, and an encoding protein, a recombinant vector and an application of the wheat stem rot regulatory gene Tatrx-m. The genomic sequence of the gene is SEQ ID NO.1, the CDS sequence of the gene is SEQ ID NO.2, and thioredoxin shown as SEQ ID NO.3 is encoded. The invention further provides a specific primer pair used for gene identification, a silent vector (Tatrx-m-VIGS) and an overexpression vector (LGY-OE3-Tatrx-m) are constructed, and the function of the primer pair is verified through a virus-induced gene silencing (VIGS) technology and agrobacterium-mediated transformation of wheat. Experiments show that silence of Tatrx-m results in significant reduction of wheat stem rot resistance, and overexpression of the gene can improve resistance. Based on hypha quantification, DAB staining, H2O2 content and cell death analysis, Tatrx-m is revealed to enhance wheat resistance by regulating active oxygen removal and cell wall strengthening pathways. The gene TaTrx-m has the positive regulation effect in wheat stem rot for the first time, can be applied to disease-resistant molecular marker development, gene editing and transgenic breeding, provides core genetic resources for disease-resistant variety breeding, and has theoretical and application values.

The invention relates to application of tryptophol in prevention and treatment of corn bacterial stem rot and relates to the technical field of biological prevention and control, the pathogenic bacterium of the corn bacterial stem rot is Dickeya zeae, it is found for the first time that tryptophol can inhibit the mobility of the Dickeya zeae, reduce the activity of cell wall degrading enzyme and relieve the disease degree, and the tryptophol can inhibit the activity of the Dickeya zeae, so that the tryptophol can be used for preventing and treating the corn bacterial stem rot. The tryptophol can effectively reduce the pathogenicity of tryptophol, but has no adverse effect on the cell viability and form of the corn dibasic bacteria, which indicates that the effect of tryptophol on the corn dibasic bacteria is realized by inhibiting the pathogenicity of bacteria instead of killing bacteria, so that tryptophol acts on the corn bacterial stem rot, the drug resistance is not easy to generate, and the tryptophol can be used for treating the corn bacterial stem rot. The tryptophol is expected to become a substitute of antibiotics and is used for preventing and treating the corn bacterial stem rot, the diseases can be effectively controlled, the selective pressure of drug resistance development can be relieved, and a new solution is provided for sustainable prevention and treatment of the corn bacterial stem rot.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

The invention belongs to the technical field of plant biology, and particularly relates to wheat stem rot related protein TaNDR1 and application thereof. According to the invention, a gene with a non-microspecies specific disease resistance function is cloned from wheat and is named as TaNDR1. According to the invention, a barley stripe mosaic virus induced vector for silencing the TaNDR1 gene in wheat is constructed, the expression of the TaNDR1 gene is reduced, a silent TaNDR1 wheat plant is obtained, and after the silent TaNDR1 wheat is inoculated with stem rot pathogen, the stem rot resistance of the wheat is obviously reduced. Therefore, after the expression quantity of the TaNDR1 in the susceptible wheat plant is increased, the stem rot resistance of the susceptible wheat plant can be obviously improved, so that the gene can be used for cultivating the stem rot resistant wheat.

The invention relates to the technical field of microorganisms, in particular to penicillium LDFST10 and application of the penicillium LDFST10 in promoting plant growth or preventing and treating stem rot. The preservation number of the penicillium LDFST10 is CGMCC (China General Microbiological Culture Collection Center) No. 41960. The application comprises the step of applying the penicillium LDFST10 or the fermentation product thereof to rhizosphere soil of the plant so as to promote the growth of the plant and prevent and treat basal stem rot. The invention researches and screens out the penicillium frutescens LDFST10 capable of promoting plant growth and inhibiting various pathogenic bacteria of stem rot, various growth promoting preparations or bacteriostatic agents or drugs aiming at the stem rot can be developed on the basis of the penicillium frutescens, and the penicillium frutescens LDFST10 has important value in the planting field of various crops such as corn, wheat and the like.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

The invention discloses bacillus cereus L15, and relates to the technical field of microorganisms, the bacillus cereus L15 is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 2, 2025, and the preservation number is CGMCC No.33276. The bacillus cereus L15 has the advantages that the bacillus cereus L15 can be used for preparing the bacillus cereus L15; the new bacillus cereus L15 provided by the invention has obvious broad-spectrum antibacterial property, can have a good inhibition effect on rhizoctonia dinuclear, fusarium and phaseolus vulgaris, and can be used for preventing and treating plant root rot and stem rot caused by the fusarium, the rhizoctonia and the phaseolus vulgaris.

The invention belongs to the technical field of plant biology, and particularly relates to application of TaC2H2 protein in wheat stem rot resistance. According to the invention, a barley stripe mosaic virus inducible vector for silencing the TaC2H2 gene in wheat is constructed, the expression of the TaC2H2 gene is reduced, a silent TaC2H2 wheat plant is obtained, and the stem rot resistance of the silent TaC2H2 wheat is obviously reduced after the silent TaC2H2 wheat is inoculated with stem rot pathogens. Therefore, the TaC2H2 gene plays a positive role in wheat stem rot resistance regulation and control, so that the gene can be used for cultivating stem rot resistant wheat.

Methods and compositions for identifying, selecting and / or producing a pathogen resistant plant, plant cell or seed (e. g,, a maize plant, plant cell or seed) are provided. Polynucleotides and polypeptides are provided, which when expressed in a plant increase disease and / or pathogen resistance (e.g., to Fusarium stalk rot) of the plant. Plants, plant cells and seed are provided comprising such polypeptides and polynucleotides, as are methods for their use.

The invention provides an application of a corn disease-resistant gene ZmCRK10 and a disease-resistant molecular marker thereof in improvement of corn stem rot resistance, and belongs to the technical field of plant genetic engineering. The invention provides application of a disease-resistant gene ZmCRK10 or a disease-resistant protein ZmCRK10-T03 in improvement of corn stem rot resistance. The disease-resistant gene ZmCRK10 or the disease-resistant protein ZmCRK10-T03 can be used for constructing a transgenic corn variety resistant to corn stem rot. The invention further provides application of the disease-resistant molecular marker ZmCRK10TypeB of the corn disease-resistant gene ZmCRK10 in improving the corn stem rot resistance, and effective auxiliary molecular marker selection can be provided for corn stem rot resistance breeding.

The invention provides a method for improving the disease resistance of plants, and particularly provides a method for improving the disease resistance of the plants by reducing or inhibiting the expression quantity and / or activity of SWEET11a genes or encoded proteins thereof. According to the method, the disease resistance of the plants can be improved, particularly the resistance to gray speck disease, northern leaf blight, bacterial wilt and stem rot is improved, and the method has important application value in biological breeding.

The invention provides streptomyces LZZY-S3 and application thereof, and belongs to the field of microorganisms. The streptomyces LZZY-S3 provided by the invention enriches strains for biological control, and realizes the dual functions of plant pathogenic bacterium resistance and plant growth promotion. The streptomyces LZZY-S3 has a broad-spectrum fungus inhibition function and can be applied to plant pathogenic bacterium resistance; pathogenic bacteria include: European tea leaf disease pathogen, Botryosphaeria spp., Gibberella zeae pathogen, fusarium oxysporum pathogen, wheat root rot pathogen, rice seedling rot pathogen, pumpkin gummy stem blight pathogen, wheat stem rot pathogen, Trichothecium roseum, grape white rot pathogen, and Colletotrichum graminum pathogen. The pathogenic bacteria include: European tea leaf disease pathogen, Botryosphaeria spp. Wherein the inhibition rate of tea cake disease germs reaches 91.2%. The streptomyces LZZY-S3 can also be applied to plant growth promotion, preparation of nitrogen-fixing bacterial agents, preparation and production of ACC deaminase bacterial agents and the like.

The present invention relates to the technical field of molecular markers, in particular to a QTL for regulating the corn stalk rot resistance, an SNP molecular marker closely linked therewith, and the use thereof. The QTL for regulating the corn stalk rot resistance provided herein can be used for improving the corn stalk rot resistance and performing breeding of stalk rot-resistant corns. The provided SNP molecular marker closely linked with the corn stalk rot-resistant QTL can be used for identifying the stalk rot resistance of corns and has high accuracy, and can also be used for assisted breeding and germplasm resource improvement for the corn stalk rot resistance, thus achieving an important significance in improving the breeding efficiency and breeding level in stalk rot-resistant corns.

The invention discloses bacillus siamensis and application thereof in prevention and treatment of corn stem rot. Relates to the technical field of biocontrol. The preservation number is CGMCC (China General Microbiological Culture Collection Center): NO.33096. The invention also provides a fermentation product and application of the strain. The traditional Chinese medicine composition has a good inhibition effect and a relatively wide antibacterial spectrum. In a field experiment, the fungicide disclosed by the invention has a relatively good prevention and treatment effect on fusarium graminearum after being applied, so that the attack degree of corn stem rot is effectively reduced, and the yield of corn is remarkably increased. The bacillus siamensis JLB07 strain can play a role in agricultural production in various forms, a liquid agent (bacterial liquid) can be used for root irrigation, powder can be used for seed dressing, and a biological compound fertilizer prepared from the bacillus siamensis JLB07 strain has the effects of remarkably reducing the corn stem rot disease, increasing the yield and improving the quality of a product.

The invention belongs to the field of plant biotechnology and plant molecular breeding, and particularly relates to a brassica napus BnabZIP1 gene and application thereof in prevention and control of sclerotinia rot of colza. According to the invention, a gene editing technical vector is constructed aiming at sgRNA of the BnabZIP1 gene, agrobacterium tumefaciens is transformed, then brassica napus is transfected, and a homozygous mutant plant after the BnabZIP1 gene is knocked out has stronger sclerotiniose resistance. Results show that the BnabZIP1 gene can be used as a molecular marker or a genetic engineering improvement target of sclerotiniose resistance of rape, is used for screening and creating disease-resistant rape varieties, and has a good molecular breeding application prospect.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.