145 results about "Growth medium" patented technology

An electrochemical cell has an active alloy anode including an active alloy and a passive alloy cathode including a passive alloy with the active alloy having a higher reduction potential than the passive alloy within growth media. The active alloy anode and the passive alloy cathode are positioned to drive a plurality of transport ions into a plant in some embodiments to enhance plant growth and to kill weeds in other embodiments.

The invention relates to the technical field of preparation of rice seedling raising substrates, in particular to a preparation method and application of a rice seedling raising substrate based on rapid fermentation of reed foam, and the rice seedling raising substrate comprises the following components in parts by weight: 35-50 parts of reed residue, 15-22 parts of cow dung, 8-15 parts of carbonized rice husk, 10-20 parts of turf, 13-22 parts of modified bacterium-loaded vermiculite and 3-8 parts of a nutritional composition, the modified bacterium-loaded vermiculite comprises nitrogen-fixing bacteria and a vermiculite carrier subjected to functional modification. According to the invention, humic acid and other active substances generated in the composting fermentation process of reed residues and cow dung can chelate metal ions in the nutritional composition, the bioavailability of the nutritional composition is improved, and carbonized rice husks, turf and bacterium-loaded vermiculite construct a composite substrate system with excellent physical properties and strong biological functions. Organic unification of nutrition supply, physical support and biological regulation is realized, and an ideal growth medium is provided for cultivating robust rice seedlings suitable for mechanical transplanting.

A living paint comprises a living photosynthetic microorganism, binder, growth medium, and water. The microorganism may comprise algae, cyanobacteria, or lichens. The binder may be composed of primary

A system for producing a seed and / or fertilizer filled plant growth medium pot, rod, or band is provided. The system includes a supporting means (adapted for receiving and supporting a plant growth medium pot, rod, or band), a feeding tube adapted to be in a retracted position or an extended position (for insertion into the growth medium), and a delivery unit (adapted for delivering a seed and / or a dosage of fertilizer through the feeding tube and into the growth medium). The feeding tube includes a cutting means, preferably a knife blade, mounted at the tip of the feeding tube. The cutting means is adapted for cutting into the plant growth medium pot, rod, or band, thereby facilitating the movement of the feeding tube into the plant growth medium pot, rod, or band.

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to improve the growth of various farming plants exposed to acidity stress by selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then infused to the soil or other growth medium. Optionally, the KHP solution can be diluted by water, as disclosed in the specification, for applying to the soil or other growth medium.

The application discloses application of a coumarin compound or a composition thereof in inhibition of a plant Huanglongbing pathogen, prevention of plant Huanglongbing, improvement of plant resistance to Huanglongbing, inhibition of a plant pathogenic bacterium and preparation of a product for preventing and treating plant Huanglongbing, improving plant Huanglongbing resistance, inhibiting a plant Huanglongbing pathogen and inhibiting a plant pathogenic bacterium. The coumarin compound is isofraxidiformin or ostruthin. By applying the coumarin compound or the composition thereof to a receptor plant or a growth medium thereof, the content of the coumarin compound or the composition thereof in the receptor plant is increased, and then the citrus Huanglongbing pathogen in the plant body is significantly reduced C Las quantity. Under field application conditions, the coumarin compound or the composition thereof also shows good prevention and treatment potential. In addition, the coumarin compound shows broad-spectrum bacteriostatic activity on a plurality of other plant pathogenic bacteria, and shows good plant antibacterial prevention and treatment potential.

A vertical farming system includes: a plurality of grow trays for receiving a growth medium for germinating, propagating and / or growing living organisms, each grow tray including a bottom tray surface; a plurality of support units, each support unit including a single platform including+ a top platform surface for supporting the bottom tray surface of a grow tray from below, wherein each bottom tray surface and / or each top platform surface are configured such that when a grow tray is supported on top of a support unit, a ventilation space is defined between the bottom tray surface and the top platform surface. Each support unit includes a fan mounted to the platform, the fan being configured to generate an airflow through the ventilation space. A support structure supports the plurality of support units in a vertically spaced arrangement.

Tray assemblies for germinating and growing plant seeds are provided. Preferably, the tray assemblies comprise outer trays, inner trays, inner divider frames, and covers. The dividers or partitions of the inner divider frame form a plurality of compartments across the frame. Each compartment is configured to receive at least one seed for germination and growth. Seeds are placed in in the compartments and allowed to float in water and / or sit in humid air. A cover is placed over the divider frame to block-out light and retain heat. Once the seeds have germinated, they can be placed in a growth medium that helps promote the plant growth and development.

Systems and Methods for determining response of microbe cultures to antimicrobials by measuring properties of individual microbes and / or other culture particles. Methods include incubating microbes in liquid cultures which may contain antimicrobials and growth media, detecting individual microbes and particles, and measuring their properties, which may include the mass of individual microbes and particles. Measurement data, such as masses of a plurality of individual microbes or particles, may be collected after an incubation duration. These data may be analyzed to determine susceptibility of microbes to one or more antibiotics. Measurement may be performed after incubation durations shorter than required to produce growth by microbe replication, and analysis may include assessment of metrics other than the growth of the culture. The method enables measurement of susceptibility at earlier times than needed for sufficient microbe replication required by many existing techniques enabling accurate results with relatively minimal sample volumes of a culture.

A microbiological testing device includes a growth medium support plate and a cap attached to the growth medium support plate, the growth medium support plate including a microbiological testing area, wherein the microbiological testing area includes at least one recess for receiving a microbiological growth medium, wherein the least one recess includes a bottom surface and at least one anchoring element protruding from the bottom surface, and wherein the at least one anchoring element includes an undercut for engaging with the microbiological growth medium. Further, a microbiological testing assembly and a cap for the microbiological testing device are described.

A bioremediating composition including a consortial culture with at least one fungal culture and at least one bacterium culture, a growth medium with a waste material that includes synthetic rubber and method for producing a biodegradable byproduct from a waste material that includes synthetic rubber, preparing the growth medium with the waste material, and incubating the growth medium with at least one bioremediating composition with at least one fungal and one bacterium culture.

There are disclosed processes and systems of culturing and mixing algal growth medium in at least one open algal aquaculture pond for a continuous flow system without requiring mechanical mixing devices. The process involves constructing an open algal aquaculture pond possessing a fetch selected as a function of a specified wind speed and a wave mixed layer (WML) defined by a specified depth from the surface of the pond; supplying algal growth medium to the open algal aquaculture pond, the pond having at least partially a manmade configuration; and culturing algae in the algal growth medium in the open algal aquaculture pond; and wherein a ratio of the WML of the algal growth medium to a total depth of the algal growth medium in the pond is greater than about 0.2. Also disclosed are uses of the mixed algal aquaculture medium and systems for culturing algae.

The invention provides a rice seedling raising substrate sheet and a preparation method thereof and a seedling raising method, and belongs to the technical field of rice seedling raising, and the preparation method comprises the following steps: mixing decomposed straw, an adhesive and biochar, spraying water to adjust the water content to be 50-60%, and performing hot pressing at the temperature of 120-150 DEG C to form a latticed separator; thoroughly decomposed straw, thoroughly decomposed livestock and poultry manure and water are mixed and stirred and then ground into slurry, the pH is adjusted to 6.0-6.5, and substrate slurry is obtained; and filling meshes of the latticed separator with the matrix slurry, carrying out vacuum suction filtration molding, and drying to obtain the rice seedling raising matrix sheet. The matrix slurry in the rice seedling raising matrix sheet adopts an acid regulation and nutrient controlled release technology, a stable and coordinated growth medium with a water, gas and fertilizer structure can be provided for seedlings, meanwhile, root systems of the seedlings are orderly separated by the latticed separators in the growth process, the root systems of the seedlings are coiled in meshes, the root system activity is high, and the quality of the seedlings is good.

There are disclosed processes and systems of culturing and mixing algal growth medium in at least one open algal aquaculture pond for a continuous flow system without requiring mechanical mixing devices. The process involves constructing an open algal aquaculture pond possessing a fetch selected as a function of a specified wind speed and a wave mixed layer (WML) defined by a specified depth from the surface of the pond; supplying algal growth medium to the open algal aquaculture pond, the pond having at least partially a manmade configuration; and culturing algae in the algal growth medium in the open algal aquaculture pond; and wherein a ratio of the WML of the algal growth medium to a total depth of the algal growth medium in the pond is greater than about 0.2. Also disclosed are uses of the mixed algal aquaculture medium and systems for culturing algae.

A method for large-scale expansion of natural killer (NK) cells in a cell culture medium according to the invention, comprising the steps of: adding said cells to a culture system comprising a growth medium supplemented with serum selected from the group consisting of human serum and autologous plasma; cRX 527 with an effective concentration is added; the cells are expanded and heated within the system wherein the effective concentration of CRX 527 in the growth medium is in the range of 0.01 [mu] g / ml to 10 [mu] g / ml, preferably 100 ng / ml, and the ratio between the cell density and the total volume of the growth medium is in the range of 1.5 to 2.

The present invention relates to the production of propagation plugs, primarily blocks of growth medium for the growing of cuttings and seed plants, and of the type consisting of a cylindrical block having an envelope of sheet material and an associated filling of sphagnum or a corresponding substrate material. The sheet material is here a netting or mesh, preferably biodegradable, having a mesh size within the range of 0.1 to 1.5 mm.

The application discloses a long-term in-vitro preservation method for endangered Dendrobium germplasm, which comprises the following two steps: a first stage of inhibiting growth medium for long-term preservation of sterile Dendrobium seeds, and a second stage of recovery growth medium for seedling induction culture; the inhibiting growth medium formula is oat 40-80 g / L+ agar 6 g / L; and the recovery growth medium formula is 1 / 2MS+ peptone 1.0 g / L+ activated carbon 1.0 g / L+ sucrose 25 g / L+ agar 6.0 g / L+ potato 50 g / L+ banana 80 g / L+ apple 15 g / L. The method can realize long-term preservation and utilization of endangered Dendrobium germplasm resources by using organic additives, preparing suitable culture medium, adjusting the pH value and the like, and has the advantages of high preservation rate, long preservation time, low cost, good germplasm traits and the like.

The invention relates to a method of producing an ester of a fatty acid and a C1-C4 alcohol, comprising successive steps of: cultivating cells of a microalga strain on a growth medium, harvesting the cultivated cells and concentrating them to obtain a wet cell pellet, suspending said wet cell pellet in an hydroalcoholic solution comprising said C1-C4 alcohol, incubating the cell suspension thus formed so as to form said ester of a fatty acid and a C1-C4 alcohol by transesterification and / or esterification of lipids produced by the microalga cells catalyzed by endogenous enzymes of said cells, and recovering said ester of a fatty acid and a C1-C4 alcohol from said cell suspension. The growth medium can advantageously be a dark fermentation effluent.

The invention belongs to the technical field of cosmetics, and discloses a saussurea involucrate callus extract as well as a preparation method and application thereof. The preparation method of the saussurea involucrata callus extract comprises the following steps: inoculating saussurea involucrata into an induction culture medium for culturing to obtain saussurea involucrata callus; inoculating the saussurea involucrata callus into a growth culture medium for culturing, and centrifuging to obtain saussurea involucrata suspension cells; and mixing the saussurea involucrata suspension cells with an extraction solvent, and performing high-pressure homogenization to obtain the saussurea involucrata callus extract. According to the preparation method disclosed by the invention, callus culture, high-pressure homogenization and the deep-eutectic solvent are combined, the efficiency of obtaining active ingredients in the saussurea involucrata callus is improved through a green process, and the saussurea involucrata callus extract with a remarkable anti-inflammatory effect is prepared.

A liquid-chemical sterilization system with a biological indicator is disclosed. The sterilization system includes a vial, a carrier of microorganisms disposed in the vial, and a fluid management system having an output connectable to the vial for delivering a liquid chemical sterilant and a neutralizing agent to the vial. Additionally, a growth medium can be delivered to the vial so that the vial and growth medium can be evaluated for a change in a visual characteristic indicative of a sterilization failure.

This invention provides a method for the germination and regeneration of mature rubber tree embryos into seedlings outside of test tubes, belonging to the field of seedling cultivation technology. The method uses somatic embryos derived from anther tissue culture as raw materials, and involves three stages of cultivation in a growth medium: dark culture, low-light culture, and strong-light culture. Then, the mature embryos that meet the requirements undergo closed-bottle / open-bottle acclimatization. After this acclimatization, they can be rooted and grown into seedlings in river sand outside the test tube. This method allows mature embryos to germinate and regenerate directly into seedlings outside the test tube, eliminating the need for a sterile culture room and specialized rooting medium, thus requiring less specialized knowledge from the operator. Furthermore, by combining the embryo germination and seedling regeneration with the in-test-tube and sand-bed acclimatization in one step, the cultivation process is simplified, the cultivation cycle is shortened by two months, and cultivation efficiency is improved while production costs are reduced.

Disclosed herein is a system for self-administering an infection culture test, the system including a housing having a cover and a compartment for storing a bacterial culture and to facilitate bacterial growth, wherein the compartment includes a pad having a partitioning element attached to a film, wherein the partitioning element separates a dry chromogenic medium into regions, wherein the medium is coated with a growth medium and a chromogenic substrate, and a transparent cover to hermetically seal the compartment, and one or more smart devices configured to record one or more images of the bacteria within the apparatus and provide a determination whether an infection is present and to identify what specific bacteria is present.

The disclosure is directed to a method for seed train expansion of adherent cells comprising culturing cells with a serum-supplemented growth medium in a N-2 vessel; removing the cells from the serum-supplemented medium; inoculating the cells from step into a serum-free growth medium in a N-1 vessel; culturing the cells in the N-1 vessel under suspension conditions; and inoculating a growth medium in a bioreactor with the suspension-cultured cells. In some aspects, the adherent cells are not suspension-adapted. In some aspects, the adherent cells are suspension-adapted. In some aspects, the adherent cells produced by the seed train expansion method are used to produce viral vectors. In some aspects, the viral vectors are AAV vectors.

The application discloses application of a microbial composition in industrial hazardous waste treatment and belongs to the technical field of industrial hazardous waste treatment and comprises the following steps: S1, collecting industrial hazardous waste, obtaining hazardous waste materials after pretreatment of the industrial hazardous waste, and filling the hazardous waste materials in a fermentation tank; S2, adding a growth medium in a bioreactor, connecting domesticated bacterial liquid to the bioreactor for expansion culture, and obtaining fermentation materials of compound acidophilic bacteria; S3, adding a biosurfactant to the fermentation materials of the compound acidophilic bacteria, uniformly stirring, and obtaining cell membrane surface hydrophobic modified compound acidophilic bacteria; and S4, then adding an acidic pH buffer solution to obtain a to-be-treated material. The application can maintain the stability of the pH required by the acidophilic bacteria in the process of treating the industrial hazardous waste and improve the cell membrane surface hydrophobicity of the compound acidophilic bacteria.

The invention pertains to a growth medium composition selective for filamentous fungi, its use in quality control and / or for detecting filamentous fungi in a sample, a method for detecting filamentous fungi in a sample and a kit-of-parts to prepare the growth medium composition.

An inverted conical bioreactor is provided for growing cells or microorganisms. The bioreactor has an internal space and a perforated barrier within the vessel, through which a liquid may flow, where cells or microorganisms cannot pass through the perforated barrier. The perforated barrier divides the internal space of the bioreactor into a first chamber and a second chamber. Cells are grown within the second chamber and can be perfused by re-circulating the liquid, for example a growth medium, through the bioreactor. Various inlet ports and outlet ports allow controlling the parameters of flow of the growth medium.

The invention relates to methods for extracting, purifying and concentrating mesenchymal stromal cell exosomes enriched with micro-RNA, proteins and lipids, and can be used in cosmetology and pharmacology for rehabilitation and regeneration. The technical result of the invention is a more efficient process for producing exosomes. This technical result is achieved in that the present method for extracting, purifying and concentrating exosomes from mesenchymal stromal cells includes cultivating cells, collecting the conditioned culture medium, depleting high-molecular-weight proteins, extracting, purifying and concentrating exosomes, and freezing and lyophilizing the exosome concentrate; in order to enrich the culture medium with exosomes, multipotent mesenchymal stem cells (MSCs) are grown in a medium having a lowered glucose concentration and containing platelet lysate and 5 mM of L-alanyl-L-glutamine, wherein, in the case of static cell cultivation, the growth medium is placed in a culture vessel and once a level of not less than 80% monolayer confluence is reached, the culture medium is harvested and replaced once every 24 hours, and in the case of flow-through cell cultivation, cells are placed on cell carriers in a bioreactor and 72-96 hours after the start of cultivation, the culture medium is harvested and replaced once every 24 hours, the exosome-enriched culture medium is subjected to concentration, which includes successive stages of centrifugation, tangential flow filtration and isopycnic centrifugation: in the first stage, the culture medium is centrifuged for 10 mins at 2000 g to remove large cell particles and other inclusions; in the tangential flow filtration stage, the supernatant is concentrated using hollow fibre filters having a molecular weight cutoff of 500 kDa, after which the concentrate is subjected to fivefold diafiltration with an equal volume of DPBS phosphate buffer solution using the same hollow fibre cartridge; and in the isopycnic centrifugation stage, the concentrated supernatant is mixed with a saccharose solution and deuterium oxide and subjected to isopycnic centrifugation at 100000 g and 4˚С for 75 mins, after which the concentrate is subjected to further fivefold diafiltration with a buffer solution, then the concentrate is filtered through filters with a pore diameter of 0.22 µm and 0.1 µm, the exosome concentrate is subjected to initial freezing to -75˚C in a freezing chamber, and drying is carried out in a lyophilization chamber, wherein in a first drying stage, the concentrate is cooled to -40˚С and subsequently held for 100 mins at an absolute pressure in the chamber of not more than 10 Pa, then cooled to -30˚С and subsequently held for 610 mins at an absolute pressure in the chamber of not more than 10 Pa, then cooled to -10˚С and subsequently held for 315 mins at an absolute pressure in the chamber of not more than 10 Pa, after which it is cooled to 0˚С and subsequently held for 130 mins at an absolute pressure in the chamber of not more than 10 Pa, then heated to +10˚С and subsequently held for 130 mins at an absolute pressure in the chamber of not more than 10 Pa; in a second drying stage, drying is carried out at +22˚С for not less than 130 minutes at an absolute pressure in the chamber of not more than 10 Pa.

Loose growth media, such as potting mixtures, that support the growth of plants and crops are disclosed. At least a portion of the natural components (e.g., peat, coconut fiber) of the potting mixture is replaced with an artificial component in the form of a glass fiber material.

A propagation stopper comprising a radiated hydrophilic matrix body comprising a growth medium with bound organic fibers, the matrix body being rewettable and having a moisture content of less than about 20% by weight based on the total weight of the body, as measured according to ASTM Test Method D2216, and being sterile, as measured according to ASTM Test Method D2216, and having a moisture content of less than about 20% by weight based on the total weight of the body. The number of aerobic bacteria measured by the number 4833-1 of the ISO test method is lt; 10 cfu / g.

In one aspect the invention provides a process of fabricating a growing media involving providing a mould. The mould defines a shape. The process deposits a defined amount of media selected for characteristics into the mould. The media is wetted. The media is compressed in the mould. The process agitates the media while compressed. The process needles the media while wet so as to promote felting and create structure. The media is dried to provide a growing media with engineered layers of media of selected characteristics deposited iteratively.