76 results about "Growth medium" patented technology

A living paint comprises a living photosynthetic microorganism, binder, growth medium, and water. The microorganism may comprise algae, cyanobacteria, or lichens. The binder may be composed of primary

A system for producing a seed and / or fertilizer filled plant growth medium pot, rod, or band is provided. The system includes a supporting means (adapted for receiving and supporting a plant growth medium pot, rod, or band), a feeding tube adapted to be in a retracted position or an extended position (for insertion into the growth medium), and a delivery unit (adapted for delivering a seed and / or a dosage of fertilizer through the feeding tube and into the growth medium). The feeding tube includes a cutting means, preferably a knife blade, mounted at the tip of the feeding tube. The cutting means is adapted for cutting into the plant growth medium pot, rod, or band, thereby facilitating the movement of the feeding tube into the plant growth medium pot, rod, or band.

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to improve the growth of various farming plants exposed to acidity stress by selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then infused to the soil or other growth medium. Optionally, the KHP solution can be diluted by water, as disclosed in the specification, for applying to the soil or other growth medium.

The application discloses application of a coumarin compound or a composition thereof in inhibition of a plant Huanglongbing pathogen, prevention of plant Huanglongbing, improvement of plant resistance to Huanglongbing, inhibition of a plant pathogenic bacterium and preparation of a product for preventing and treating plant Huanglongbing, improving plant Huanglongbing resistance, inhibiting a plant Huanglongbing pathogen and inhibiting a plant pathogenic bacterium. The coumarin compound is isofraxidiformin or ostruthin. By applying the coumarin compound or the composition thereof to a receptor plant or a growth medium thereof, the content of the coumarin compound or the composition thereof in the receptor plant is increased, and then the citrus Huanglongbing pathogen in the plant body is significantly reduced C Las quantity. Under field application conditions, the coumarin compound or the composition thereof also shows good prevention and treatment potential. In addition, the coumarin compound shows broad-spectrum bacteriostatic activity on a plurality of other plant pathogenic bacteria, and shows good plant antibacterial prevention and treatment potential.

Tray assemblies for germinating and growing plant seeds are provided. Preferably, the tray assemblies comprise outer trays, inner trays, inner divider frames, and covers. The dividers or partitions of the inner divider frame form a plurality of compartments across the frame. Each compartment is configured to receive at least one seed for germination and growth. Seeds are placed in in the compartments and allowed to float in water and / or sit in humid air. A cover is placed over the divider frame to block-out light and retain heat. Once the seeds have germinated, they can be placed in a growth medium that helps promote the plant growth and development.

A microbiological testing device includes a growth medium support plate and a cap attached to the growth medium support plate, the growth medium support plate including a microbiological testing area, wherein the microbiological testing area includes at least one recess for receiving a microbiological growth medium, wherein the least one recess includes a bottom surface and at least one anchoring element protruding from the bottom surface, and wherein the at least one anchoring element includes an undercut for engaging with the microbiological growth medium. Further, a microbiological testing assembly and a cap for the microbiological testing device are described.

There are disclosed processes and systems of culturing and mixing algal growth medium in at least one open algal aquaculture pond for a continuous flow system without requiring mechanical mixing devices. The process involves constructing an open algal aquaculture pond possessing a fetch selected as a function of a specified wind speed and a wave mixed layer (WML) defined by a specified depth from the surface of the pond; supplying algal growth medium to the open algal aquaculture pond, the pond having at least partially a manmade configuration; and culturing algae in the algal growth medium in the open algal aquaculture pond; and wherein a ratio of the WML of the algal growth medium to a total depth of the algal growth medium in the pond is greater than about 0.2. Also disclosed are uses of the mixed algal aquaculture medium and systems for culturing algae.

The invention provides a rice seedling raising substrate sheet and a preparation method thereof and a seedling raising method, and belongs to the technical field of rice seedling raising, and the preparation method comprises the following steps: mixing decomposed straw, an adhesive and biochar, spraying water to adjust the water content to be 50-60%, and performing hot pressing at the temperature of 120-150 DEG C to form a latticed separator; thoroughly decomposed straw, thoroughly decomposed livestock and poultry manure and water are mixed and stirred and then ground into slurry, the pH is adjusted to 6.0-6.5, and substrate slurry is obtained; and filling meshes of the latticed separator with the matrix slurry, carrying out vacuum suction filtration molding, and drying to obtain the rice seedling raising matrix sheet. The matrix slurry in the rice seedling raising matrix sheet adopts an acid regulation and nutrient controlled release technology, a stable and coordinated growth medium with a water, gas and fertilizer structure can be provided for seedlings, meanwhile, root systems of the seedlings are orderly separated by the latticed separators in the growth process, the root systems of the seedlings are coiled in meshes, the root system activity is high, and the quality of the seedlings is good.

A method for large-scale expansion of natural killer (NK) cells in a cell culture medium according to the invention, comprising the steps of: adding said cells to a culture system comprising a growth medium supplemented with serum selected from the group consisting of human serum and autologous plasma; cRX 527 with an effective concentration is added; the cells are expanded and heated within the system wherein the effective concentration of CRX 527 in the growth medium is in the range of 0.01 [mu] g / ml to 10 [mu] g / ml, preferably 100 ng / ml, and the ratio between the cell density and the total volume of the growth medium is in the range of 1.5 to 2.

The application discloses a long-term in-vitro preservation method for endangered Dendrobium germplasm, which comprises the following two steps: a first stage of inhibiting growth medium for long-term preservation of sterile Dendrobium seeds, and a second stage of recovery growth medium for seedling induction culture; the inhibiting growth medium formula is oat 40-80 g / L+ agar 6 g / L; and the recovery growth medium formula is 1 / 2MS+ peptone 1.0 g / L+ activated carbon 1.0 g / L+ sucrose 25 g / L+ agar 6.0 g / L+ potato 50 g / L+ banana 80 g / L+ apple 15 g / L. The method can realize long-term preservation and utilization of endangered Dendrobium germplasm resources by using organic additives, preparing suitable culture medium, adjusting the pH value and the like, and has the advantages of high preservation rate, long preservation time, low cost, good germplasm traits and the like.

The invention belongs to the technical field of cosmetics, and discloses a saussurea involucrate callus extract as well as a preparation method and application thereof. The preparation method of the saussurea involucrata callus extract comprises the following steps: inoculating saussurea involucrata into an induction culture medium for culturing to obtain saussurea involucrata callus; inoculating the saussurea involucrata callus into a growth culture medium for culturing, and centrifuging to obtain saussurea involucrata suspension cells; and mixing the saussurea involucrata suspension cells with an extraction solvent, and performing high-pressure homogenization to obtain the saussurea involucrata callus extract. According to the preparation method disclosed by the invention, callus culture, high-pressure homogenization and the deep-eutectic solvent are combined, the efficiency of obtaining active ingredients in the saussurea involucrata callus is improved through a green process, and the saussurea involucrata callus extract with a remarkable anti-inflammatory effect is prepared.

This invention provides a method for the germination and regeneration of mature rubber tree embryos into seedlings outside of test tubes, belonging to the field of seedling cultivation technology. The method uses somatic embryos derived from anther tissue culture as raw materials, and involves three stages of cultivation in a growth medium: dark culture, low-light culture, and strong-light culture. Then, the mature embryos that meet the requirements undergo closed-bottle / open-bottle acclimatization. After this acclimatization, they can be rooted and grown into seedlings in river sand outside the test tube. This method allows mature embryos to germinate and regenerate directly into seedlings outside the test tube, eliminating the need for a sterile culture room and specialized rooting medium, thus requiring less specialized knowledge from the operator. Furthermore, by combining the embryo germination and seedling regeneration with the in-test-tube and sand-bed acclimatization in one step, the cultivation process is simplified, the cultivation cycle is shortened by two months, and cultivation efficiency is improved while production costs are reduced.

The disclosure is directed to a method for seed train expansion of adherent cells comprising culturing cells with a serum-supplemented growth medium in a N-2 vessel; removing the cells from the serum-supplemented medium; inoculating the cells from step into a serum-free growth medium in a N-1 vessel; culturing the cells in the N-1 vessel under suspension conditions; and inoculating a growth medium in a bioreactor with the suspension-cultured cells. In some aspects, the adherent cells are not suspension-adapted. In some aspects, the adherent cells are suspension-adapted. In some aspects, the adherent cells produced by the seed train expansion method are used to produce viral vectors. In some aspects, the viral vectors are AAV vectors.

The application discloses application of a microbial composition in industrial hazardous waste treatment and belongs to the technical field of industrial hazardous waste treatment and comprises the following steps: S1, collecting industrial hazardous waste, obtaining hazardous waste materials after pretreatment of the industrial hazardous waste, and filling the hazardous waste materials in a fermentation tank; S2, adding a growth medium in a bioreactor, connecting domesticated bacterial liquid to the bioreactor for expansion culture, and obtaining fermentation materials of compound acidophilic bacteria; S3, adding a biosurfactant to the fermentation materials of the compound acidophilic bacteria, uniformly stirring, and obtaining cell membrane surface hydrophobic modified compound acidophilic bacteria; and S4, then adding an acidic pH buffer solution to obtain a to-be-treated material. The application can maintain the stability of the pH required by the acidophilic bacteria in the process of treating the industrial hazardous waste and improve the cell membrane surface hydrophobicity of the compound acidophilic bacteria.

Loose growth media, such as potting mixtures, that support the growth of plants and crops are disclosed. At least a portion of the natural components (e.g., peat, coconut fiber) of the potting mixture is replaced with an artificial component in the form of a glass fiber material.

In one aspect the invention provides a process of fabricating a growing media involving providing a mould. The mould defines a shape. The process deposits a defined amount of media selected for characteristics into the mould. The media is wetted. The media is compressed in the mould. The process agitates the media while compressed. The process needles the media while wet so as to promote felting and create structure. The media is dried to provide a growing media with engineered layers of media of selected characteristics deposited iteratively.

Provided are a growth medium and a method for growing cells.

A cultivation system for indoor farming of saffron plants (Crocus sativus) and other plants can include one or more compartments coupled with one or more climate modules which are controlled by one or more control systems. The control system can be configured to control the climate modules to provide different climate conditions to different compartments for different stages of the plants growth. Further, the temperature, humidity, and lighting in the compartments can include cyclic sequences configured to accelerate the life cycle of the plants. In addition, the plants can be trimmed, genome-specific fertilizer can be added to growth medium, absorbance pads can be used under the plant corms.

The present disclosure relates to a hydroponic growth medium comprising at least two vertical pieces, each comprising a top downwardly tapered portion and a bottom elongated portion, the pieces being configured and adapted to be assembled together to form a rooting structure having a top conical body including a top wide opening and gradually tapering downwards to a junction transitioning to the bottom narrow extension where the bottom elongated portions come into longitudinal contact with each other to form a narrow extension distal to the top wide opening; and a circular element for securing the rooting structure, wherein the at least two vertical pieces comprise at least one ester-linked polymer. Also disclosed is a method of cultivating a plant, a method of manufacturing the hydroponic growth medium, and a kit relating to the hydroponic growth medium.

A plant growing system includes a growth medium preparation unit and a plant growing unit. The growth medium preparation unit includes a supply of water that feeds water through a water treatment unit, a nutrition module, and a reactor sub-system to have the water treated and modified and added with nano-elements and other nutrition components to provide a nutrition formula. The nutrition formula is supplied to the plant growing unit that includes a growing box inside which a tank is provided for receiving and holding the nutrition formula. A plant plate is disposed on the tank and is formed with at least one through opening for receiving and holding a plant. The root of plant is allowed to extend into the nutrition formula inside the tank, while the stem of the plant is growing in a void space above the plant plate and inside the growing box.

This invention belongs to the field of biology and discloses a method for co-culturing enteric glial cells (EGCs) and intestinal organoids. Primary cultured and purified enteric glial cells are digested and seeded onto the membrane of a Transwell chamber. Intestinal organoids are placed in individual Matrigel drops in a co-culture plate. The Transwell chambers are placed in the co-culture plate, and growth medium is added to ensure free exchange of the growth medium through the Transwell membrane. The EGC-organoid co-culture system obtained based on this method provides a model for studying diarrhea and intestinal health in large mammals. It also lays the research foundation for developing innovative intervention strategies targeting the enteric nervous system and provides a research model for further exploring the regulatory roles of nutrients or related genes in intestinal health.

The application belongs to the technical field of plant cultivation and particularly relates to a Blumea riparia growth medium, a tissue culture medium, a tissue culture method and application. The Blumea riparia growth medium takes MS medium as a basic medium and further comprises 0.2-0.3 mg / L NAA, 0.08-0.16 mg / L DA-6, 10 mg / L vitamin C, 20 mg / L myo-inositol, 30 g / L sucrose and 6 g / L agar. DA-6 and NAA are jointly applied to improve the rooting efficiency, root length and plant height of Blumea riparia. The Blumea riparia germplasm with high content of levorotatory borneol is used as an explant to carry out tissue culture and seedling raising, the Blumea riparia tissue culture seedling has high content of levorotatory borneol, and Blumea riparia stable genetic good seedlings can be produced.

An apparatus includes a processing chamber configured to contain a growth medium, at least one reservoir configured to contain at least one fluid, and a plasma reactor. The plasma reactor includes a plurality of electrodes including a first electrode and a second electrode, and a reaction zone is disposed between the first electrode and the second electrode. The apparatus also includes an ignition unit electrically connected to at least one of the plurality of electrodes, an injector in fluid communication with the at least one reservoir, and a pressure regulator configured to transfer the fluid to the injector. Various other apparatuses, systems, and methods are also disclosed.

The application discloses a tissue culture and rapid propagation medium and method for African marula fruits, which is composed of lateral bud germination medium, cluster bud induction medium, cluster bud growth medium and rooting medium; 1L of the lateral bud germination medium is composed of MS medium, 0.05-0.07 mg of TIBA, 0.1-0.8 mg of GA3 and 3-10 g of agar; 1L of the cluster bud induction medium is composed of MS medium, 0.03-0.15 mg of TIBA, 0.2-1.2 mg of ZT, 0.3-0.8 mg of NAA, 20-40 g of sucrose and 3-10 g of agar; and the cluster bud growth medium is added with phosphate fertilizer on the basis of the cluster bud induction medium. The propagation coefficient of the method can reach 6.8 at most, the propagation effect is good, and the rooting rate of the marula fruit tissue culture seedlings is up to 95% or above. The application solves the problem of local planting of the African marula fruits.

The invention relates to a system for distributing a growth media to plants. The system comprises a container for receiving the plants, a first conduit for fluidly connecting the container to a reservoir having the growth media, and a suction device fluidly connected to the container. The suction device is operable to draw air from the container so as to cause the growth media to flow from the reservoir into the container and at least partially immerse the plants.

To provide a method for Bordetella fermentation and virulence factor production, especially PT production, for large-scale production, and in particular, to provide a method for medium adaptation performed before inoculation.SOLUTION: A method of producing a conditioned growth media comprising the steps of a. providing a sterile growth media; b. holding the sterile growth media at a temperature between about 28 to about 35 °C. for about 20 to 35 hours; and c. agitating and / or aerating the sterile growth media to generate a volumetric oxygen transfer coefficient (kLa) of about 10h-1 to about 130h-1, thereby providing a conditioned growth media.SELECTED DRAWING: Figure 1

A method and kit for for determining of an effective concentration of an antimicrobial agent for inhibiting bacterial growth are provided. The method and kit involve the use of a first and second multiwell receptacle. A fluid sample containing or suspected to contain one or more microbial organisms is dispensed into the wells of the first multiwell receptacle containing a range of dilutions of an antimicrobial agent, growth medium and a bacteriophage to form assay mixtures in the wells. The assay mixtures are then transferred to the corresponding wells of the second multiwell receptacle for detection.

The present invention relates to systems and methods for managing plant irrigation schedules. The system includes an outer housing and an inner housing disposed in the outer housing. The inner housing receives growth media for supporting a plant. The system includes a first fluid reservoir and a second fluid reservoir. The second fluid reservoir is fluidically coupled to the first fluid reservoir via a control valve. The system further includes a plurality of sensors configured to detect ambient parameters of the growth environment and one or more parameters of the growth media. The system includes a control circuitry operatively coupled to the control valve, the plurality of sensors, and a terminal device. The control circuitry determines a fluid dispensing schedule from predefined plant profiles stored in the terminal device and operates the control valve to dispense the fluid to the growth media based on the fluid dispensing schedule.

Disclosed herein are methods useful for determining the susceptibility of a bacterial strain to a siderophore beta-lactam in combination with a beta-lactamase inhibitor in an iron-containing growth medium. Disclosed herein are methods for determining the susceptibility of a bacterial strain to a siderophore beta-lactam in combination with a beta-lactamase inhibitor. In some embodiments, the method for determining the susceptibility of a bacterial strain to a siderophore beta-lactam comprises growing the bacterial strain in a growth medium having an iron concentration greater than 1 μM, treating the bacterial strain with a beta-lactamase inhibitor and a siderophore, and determining the minimum inhibitory concentration of the siderophore for the bacterial strain.