206 results about "Growth medium" patented technology

The invention discloses a method for improving lipid droplet generation and promoting the differentiation process in the bovine adipocyte differentiation process, which comprises the following steps: (1) recovering primary bovine adipocytes, and culturing in a complete growth culture medium; (2) performing induced differentiation on the bovine adipocytes in an induced differentiation culture medium; and (3) replacing the bovine adipocytes subjected to induced differentiation with a culture medium containing 50-100 [mu] mol / L of linoleic acid, and culturing the bovine adipocytes. It is found for the first time that after bovine adipocyte differentiation is finished, culture continues to be conducted in a culture medium added with LA, lipid droplet generation in the bovine adipocyte differentiation process is improved, and the differentiation process is promoted.

The present invention is directed at a method to produce a substantial number of seeds (e.g., 10,000) from a single plant. The method involves growing a mother plant under conditions designed to substantially maintain the mother plant in a vegetative state and substantially delay reaching reproductive stage. Then, removing the apical meristem once the mother plant has reached a desired stage of growth. The method further involves removing one or more branches from the mother plant (as branch cuttings). The method additionally involves maintaining each of the branch cuttings in growth media under acclimation conditions until each of the branch cuttings resume growing vegetatively as clone plants, then inducing the clone plants to flower and once the clone plants have been induced to flower, returning them to vegetative growth conditions. The clone plants are then maintained under vegetative growth conditions until they have produced a desired number of seeds.

The present disclosure provides a raft design for use in hydroponically growing crop seeds. The raft has a plurality of ribs that have a plurality of furrows between them. Media and crop seeds are placed in the furrows. The ribs have rounded tops so that the media and crop seeds do not get stuck on the top of the rib, but rather fall into the furrows. The raft also has a curved or arched underside, so that when the rafts are stacked on top of one another, air can circulate. The present disclosure also provides a process for filing the raft with growing media, seeding, and / or a topcoat. The furrows of the raft are oriented with respect to a hopper to provide a high efficiency.

The invention discloses a vertical greening system in the technical field of vertical greening. The system comprises a plant planting module, a resource collecting and preprocessing module, a seed collecting module and a seed and growth medium printing module. The resource collection and pretreatment module and the seed collection module are both connected with the seed and growth medium printing module, so that the growth medium obtained through recombination and the collected plant seeds are transmitted to the seed and growth medium printing module; the seed and growth medium printing module comprises a printing nozzle and is used for printing the recombined growth medium and the collected plant seeds on a printing carrier to form printing paper; and the transfer mechanism is used for laying the printing paper on the back surface of the vertical display wall. Environmental resources are collected, processed and recombined to form a growth medium, plant seeds are collected and placed on printing paper, the printing paper is placed behind a plant growing in the last stage, the growth period of the plant circulation and vertical plant wall is prolonged, and the maintenance and nursing cost of the vertical plant wall is saved.

The disclosure provides a growth medium and a culturing system for a nitrogen-fixing microorganism. The disclosure also provides methods of enhancing the accumulation of a microbial intracellular storage compound (MISC) in a nitrogen-fixing microorganism. The disclosure further provides biofertilizers comprising a nitrogen-fixing microorganism, as well as methods of improving and / or maintaining crop or plant yield, yield quality, or plant aesthetics, and / or improving soil health using the biofertilizers.

An electrochemical cell has an active alloy anode including an active alloy and a passive alloy cathode including a passive alloy with the active alloy having a higher reduction potential than the passive alloy within growth media. The active alloy anode and the passive alloy cathode are positioned to drive a plurality of transport ions into a plant in some embodiments to enhance plant growth and to kill weeds in other embodiments.

The invention relates to the technical field of preparation of rice seedling raising substrates, in particular to a preparation method and application of a rice seedling raising substrate based on rapid fermentation of reed foam, and the rice seedling raising substrate comprises the following components in parts by weight: 35-50 parts of reed residue, 15-22 parts of cow dung, 8-15 parts of carbonized rice husk, 10-20 parts of turf, 13-22 parts of modified bacterium-loaded vermiculite and 3-8 parts of a nutritional composition, the modified bacterium-loaded vermiculite comprises nitrogen-fixing bacteria and a vermiculite carrier subjected to functional modification. According to the invention, humic acid and other active substances generated in the composting fermentation process of reed residues and cow dung can chelate metal ions in the nutritional composition, the bioavailability of the nutritional composition is improved, and carbonized rice husks, turf and bacterium-loaded vermiculite construct a composite substrate system with excellent physical properties and strong biological functions. Organic unification of nutrition supply, physical support and biological regulation is realized, and an ideal growth medium is provided for cultivating robust rice seedlings suitable for mechanical transplanting.

A living paint comprises a living photosynthetic microorganism, binder, growth medium, and water. The microorganism may comprise algae, cyanobacteria, or lichens. The binder may be composed of primary

A system for producing a seed and / or fertilizer filled plant growth medium pot, rod, or band is provided. The system includes a supporting means (adapted for receiving and supporting a plant growth medium pot, rod, or band), a feeding tube adapted to be in a retracted position or an extended position (for insertion into the growth medium), and a delivery unit (adapted for delivering a seed and / or a dosage of fertilizer through the feeding tube and into the growth medium). The feeding tube includes a cutting means, preferably a knife blade, mounted at the tip of the feeding tube. The cutting means is adapted for cutting into the plant growth medium pot, rod, or band, thereby facilitating the movement of the feeding tube into the plant growth medium pot, rod, or band.

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to improve the growth of various farming plants exposed to acidity stress by selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then infused to the soil or other growth medium. Optionally, the KHP solution can be diluted by water, as disclosed in the specification, for applying to the soil or other growth medium.

The invention discloses application of a microbial composition in industrial hazardous waste treatment, and belongs to the technical field of industrial hazardous waste treatment.The application comprises the following steps that S1, industrial hazardous waste is collected, the industrial hazardous waste is pretreated to obtain a hazardous waste material, and a fermentation tank is filled with the hazardous waste material; s2, adding a growth culture medium into a bioreactor, inoculating the domesticated bacterial liquid into the bioreactor, and carrying out enlarged culture to obtain a fermentation material of the composite acidophilic thiobacillus; s3, adding a biosurfactant into the fermentation material of the composite acidophilic thiobacillus, and uniformly stirring to obtain the composite acidophilic thiobacillus with hydrophobically modified cell membrane surface; and S4, adding an acidic pH buffer solution to obtain a to-be-treated material. According to the method, in the industrial hazardous waste treatment process, the stability of the pH required by the acidithiobacillus acidophilus can be maintained, and the cell membrane surface hydrophobicity of the composite acidithiobacillus acidophilus is improved.

The invention relates to the technical field of plant cultivation. The invention provides a method for cultivating long fruit ginger. The method comprises the following steps: pre-treating long fruit ginger seeds; placing the pretreated long fruit ginger seeds in a culture medium containing specific hormones for culturing to obtain germinated long fruit ginger seeds; the specific hormones comprise GA, IAA and 6-BA; transferring the germinated long fruit ginger seeds into a culture medium for seedling strengthening and rooting; when the seedlings of the long fruit ginger reach a certain growth stage, seedling hardening treatment is carried out, and then the seedlings are transplanted to soil or other suitable growth media. According to the method for cultivating the zingiber longfruit, the germination rate and the germination potential of the zingiber longfruit seeds can be increased, the germination time lag of the zingiber longfruit can be reduced, and the survival rate of seedlings is increased.

This invention concerns a method for providing genetically modified cells having a defined phenotype comprising providing a microorganism or a cell of a multicellular organism, performing random mutagenesis on said microorganism or said cell such as to obtain a population of genetically modified cells, optionally, incubating the population of genetically modified cells in the absence of light between 300 nm and 500 nm for a period of resting time. In a subsequent step, culturing the cells in growth medium for a period of recovery time, encapsulating of at least a portion of the genetically modified cells in a plurality of droplets, detecting the presence of a phenotypic marker which is indicative of a defined phenotype of the genetically modified cell in the droplets, and selecting the droplets comprising the detected phenotypic marker, or comprising a quantity of the detected phenotypic marker which exceeds a predefined threshold. The invention also concerns the use of the so fabricated droplets containing the genetically-modified cells with the desired phenotype for the production of macroscopic engineered living materials or other industrial applications.

The application discloses application of a coumarin compound or a composition thereof in inhibition of a plant Huanglongbing pathogen, prevention of plant Huanglongbing, improvement of plant resistance to Huanglongbing, inhibition of a plant pathogenic bacterium and preparation of a product for preventing and treating plant Huanglongbing, improving plant Huanglongbing resistance, inhibiting a plant Huanglongbing pathogen and inhibiting a plant pathogenic bacterium. The coumarin compound is isofraxidiformin or ostruthin. By applying the coumarin compound or the composition thereof to a receptor plant or a growth medium thereof, the content of the coumarin compound or the composition thereof in the receptor plant is increased, and then the citrus Huanglongbing pathogen in the plant body is significantly reduced C Las quantity. Under field application conditions, the coumarin compound or the composition thereof also shows good prevention and treatment potential. In addition, the coumarin compound shows broad-spectrum bacteriostatic activity on a plurality of other plant pathogenic bacteria, and shows good plant antibacterial prevention and treatment potential.

The invention relates to the technical field of pepper anther tissue culture, and particularly discloses a method for improving pepper anther tissue culture embryoid induction rate and reducing pollution rate, and the method is characterized in that a culture medium containing GABA (gamma-aminobutyric acid) is adopted for culture. Specifically, the culture medium comprises an induction culture medium for inducing embryoid formation, an embryoid culture medium for culturing embryoid seedlings and a seedling growth culture medium for culturing seedlings to be mature. On the basis of a conventionally used culture medium formula, 50 mM of GABA is added into the culture medium in each stage, so that the embryoid induction rate can be remarkably improved, the pollution rate can be reduced, and finally the purpose of improving the culture success rate of the pepper anther is achieved. In the anther tissue culture process, compared with a conventional culture medium, the pollution rate of the culture medium added with 50 mM of GABA is reduced by 4.5-2.5 times, the embryoid induction rate is averagely improved by 1.6-2.2 times, and the survival haploid seedling is improved by 1.6-3.3 times.

A vertical farming system includes: a plurality of grow trays for receiving a growth medium for germinating, propagating and / or growing living organisms, each grow tray including a bottom tray surface; a plurality of support units, each support unit including a single platform including+ a top platform surface for supporting the bottom tray surface of a grow tray from below, wherein each bottom tray surface and / or each top platform surface are configured such that when a grow tray is supported on top of a support unit, a ventilation space is defined between the bottom tray surface and the top platform surface. Each support unit includes a fan mounted to the platform, the fan being configured to generate an airflow through the ventilation space. A support structure supports the plurality of support units in a vertically spaced arrangement.

Tray assemblies for germinating and growing plant seeds are provided. Preferably, the tray assemblies comprise outer trays, inner trays, inner divider frames, and covers. The dividers or partitions of the inner divider frame form a plurality of compartments across the frame. Each compartment is configured to receive at least one seed for germination and growth. Seeds are placed in in the compartments and allowed to float in water and / or sit in humid air. A cover is placed over the divider frame to block-out light and retain heat. Once the seeds have germinated, they can be placed in a growth medium that helps promote the plant growth and development.

Systems and Methods for determining response of microbe cultures to antimicrobials by measuring properties of individual microbes and / or other culture particles. Methods include incubating microbes in liquid cultures which may contain antimicrobials and growth media, detecting individual microbes and particles, and measuring their properties, which may include the mass of individual microbes and particles. Measurement data, such as masses of a plurality of individual microbes or particles, may be collected after an incubation duration. These data may be analyzed to determine susceptibility of microbes to one or more antibiotics. Measurement may be performed after incubation durations shorter than required to produce growth by microbe replication, and analysis may include assessment of metrics other than the growth of the culture. The method enables measurement of susceptibility at earlier times than needed for sufficient microbe replication required by many existing techniques enabling accurate results with relatively minimal sample volumes of a culture.

A microbiological testing device includes a growth medium support plate and a cap attached to the growth medium support plate, the growth medium support plate including a microbiological testing area, wherein the microbiological testing area includes at least one recess for receiving a microbiological growth medium, wherein the least one recess includes a bottom surface and at least one anchoring element protruding from the bottom surface, and wherein the at least one anchoring element includes an undercut for engaging with the microbiological growth medium. Further, a microbiological testing assembly and a cap for the microbiological testing device are described.

A bioremediating composition including a consortial culture with at least one fungal culture and at least one bacterium culture, a growth medium with a waste material that includes synthetic rubber and method for producing a biodegradable byproduct from a waste material that includes synthetic rubber, preparing the growth medium with the waste material, and incubating the growth medium with at least one bioremediating composition with at least one fungal and one bacterium culture.

There are disclosed processes and systems of culturing and mixing algal growth medium in at least one open algal aquaculture pond for a continuous flow system without requiring mechanical mixing devices. The process involves constructing an open algal aquaculture pond possessing a fetch selected as a function of a specified wind speed and a wave mixed layer (WML) defined by a specified depth from the surface of the pond; supplying algal growth medium to the open algal aquaculture pond, the pond having at least partially a manmade configuration; and culturing algae in the algal growth medium in the open algal aquaculture pond; and wherein a ratio of the WML of the algal growth medium to a total depth of the algal growth medium in the pond is greater than about 0.2. Also disclosed are uses of the mixed algal aquaculture medium and systems for culturing algae.

The invention provides a rice seedling raising substrate sheet and a preparation method thereof and a seedling raising method, and belongs to the technical field of rice seedling raising, and the preparation method comprises the following steps: mixing decomposed straw, an adhesive and biochar, spraying water to adjust the water content to be 50-60%, and performing hot pressing at the temperature of 120-150 DEG C to form a latticed separator; thoroughly decomposed straw, thoroughly decomposed livestock and poultry manure and water are mixed and stirred and then ground into slurry, the pH is adjusted to 6.0-6.5, and substrate slurry is obtained; and filling meshes of the latticed separator with the matrix slurry, carrying out vacuum suction filtration molding, and drying to obtain the rice seedling raising matrix sheet. The matrix slurry in the rice seedling raising matrix sheet adopts an acid regulation and nutrient controlled release technology, a stable and coordinated growth medium with a water, gas and fertilizer structure can be provided for seedlings, meanwhile, root systems of the seedlings are orderly separated by the latticed separators in the growth process, the root systems of the seedlings are coiled in meshes, the root system activity is high, and the quality of the seedlings is good.

Various embodiments relate to a culture system configured to capture, retain, and effectively maintain spores, and more particularly, to a culture system with a growth medium configured to provide efficient algae capture, attachment, and growth while providing anti-biofouling properties. The culture system may include a growth medium supported by a support structure, the growth medium having a rough surface.

There are disclosed processes and systems of culturing and mixing algal growth medium in at least one open algal aquaculture pond for a continuous flow system without requiring mechanical mixing devices. The process involves constructing an open algal aquaculture pond possessing a fetch selected as a function of a specified wind speed and a wave mixed layer (WML) defined by a specified depth from the surface of the pond; supplying algal growth medium to the open algal aquaculture pond, the pond having at least partially a manmade configuration; and culturing algae in the algal growth medium in the open algal aquaculture pond; and wherein a ratio of the WML of the algal growth medium to a total depth of the algal growth medium in the pond is greater than about 0.2. Also disclosed are uses of the mixed algal aquaculture medium and systems for culturing algae.

A method for large-scale expansion of natural killer (NK) cells in a cell culture medium according to the invention, comprising the steps of: adding said cells to a culture system comprising a growth medium supplemented with serum selected from the group consisting of human serum and autologous plasma; cRX 527 with an effective concentration is added; the cells are expanded and heated within the system wherein the effective concentration of CRX 527 in the growth medium is in the range of 0.01 [mu] g / ml to 10 [mu] g / ml, preferably 100 ng / ml, and the ratio between the cell density and the total volume of the growth medium is in the range of 1.5 to 2.

The present invention relates to the production of propagation plugs, primarily blocks of growth medium for the growing of cuttings and seed plants, and of the type consisting of a cylindrical block having an envelope of sheet material and an associated filling of sphagnum or a corresponding substrate material. The sheet material is here a netting or mesh, preferably biodegradable, having a mesh size within the range of 0.1 to 1.5 mm.

The application discloses a long-term in-vitro preservation method for endangered Dendrobium germplasm, which comprises the following two steps: a first stage of inhibiting growth medium for long-term preservation of sterile Dendrobium seeds, and a second stage of recovery growth medium for seedling induction culture; the inhibiting growth medium formula is oat 40-80 g / L+ agar 6 g / L; and the recovery growth medium formula is 1 / 2MS+ peptone 1.0 g / L+ activated carbon 1.0 g / L+ sucrose 25 g / L+ agar 6.0 g / L+ potato 50 g / L+ banana 80 g / L+ apple 15 g / L. The method can realize long-term preservation and utilization of endangered Dendrobium germplasm resources by using organic additives, preparing suitable culture medium, adjusting the pH value and the like, and has the advantages of high preservation rate, long preservation time, low cost, good germplasm traits and the like.

The invention relates to a method of producing an ester of a fatty acid and a C1-C4 alcohol, comprising successive steps of: cultivating cells of a microalga strain on a growth medium, harvesting the cultivated cells and concentrating them to obtain a wet cell pellet, suspending said wet cell pellet in an hydroalcoholic solution comprising said C1-C4 alcohol, incubating the cell suspension thus formed so as to form said ester of a fatty acid and a C1-C4 alcohol by transesterification and / or esterification of lipids produced by the microalga cells catalyzed by endogenous enzymes of said cells, and recovering said ester of a fatty acid and a C1-C4 alcohol from said cell suspension. The growth medium can advantageously be a dark fermentation effluent.

To provide a method for purifying recombinant silk polypeptide sustainably and by an environment-friendly process.SOLUTION: A method for isolating a recombinant spider silk protein from a host cell culture solution includes: a) a step of obtaining a cell culture solution, in which the cell culture solution contains a host cell and a growth medium, and the host cell expresses a recombinant spider silk protein; b) a step of collecting a part of the cell culture solution containing the recombinant spider silk protein; c) a step of incubating a part of the cell culture solution in an aqueous solution under an alkaline condition, by which the recombinant spider silk protein is solubilized in the aqueous solution; and d) a step of isolating the recombinant spider silk protein from the aqueous solution, by which an isolated recombinant spider silk protein sample is produced.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of cosmetics, and discloses a saussurea involucrate callus extract as well as a preparation method and application thereof. The preparation method of the saussurea involucrata callus extract comprises the following steps: inoculating saussurea involucrata into an induction culture medium for culturing to obtain saussurea involucrata callus; inoculating the saussurea involucrata callus into a growth culture medium for culturing, and centrifuging to obtain saussurea involucrata suspension cells; and mixing the saussurea involucrata suspension cells with an extraction solvent, and performing high-pressure homogenization to obtain the saussurea involucrata callus extract. According to the preparation method disclosed by the invention, callus culture, high-pressure homogenization and the deep-eutectic solvent are combined, the efficiency of obtaining active ingredients in the saussurea involucrata callus is improved through a green process, and the saussurea involucrata callus extract with a remarkable anti-inflammatory effect is prepared.