The invention discloses a biofilter packing composition. The biofilter packing composition comprises upper-layer packing and lower-layer packing, wherein the upper-layer packing is prepared from activated carbon, peat and sawdust, and the lower-layer packing is prepared from epidermal fiber structures of arbor plants, shells, zeolite and lava. According to the biofilter packing composition, the upper-layer packing and the lower-layer packing are adopted for layering treatment, the organic matter packing such as the activated carbon, the peat, the sawdust, the epidermal fiber structures of the arbor plants, the shells, the zeolite and the lava and inorganic matter packing are combined together, matching is scientific and reasonable, and therefore the defects that for existing biofilter packing, the deodorization process is difficult to control, nutritional substances need to be continuously put in an inert material which cannot provide nutrients, the pollutant treatment range of the peat packing is limited, large floor area is occupied, and the replacement cycle is short are simultaneously overcome.