The invention discloses a natural bait
composition analysis method for
nematode fishes and application thereof. The
analysis method comprises the steps of fish body
sample collection, gastrointestinal inclusion collection and food
composition analysis. According to the
analysis method and the application thereof, evaluation is carried out by adopting the three indexes of the weight percentage of the bait, the occurrence rate of the bait and the relative importance index of the specific food,
data redundancy of traditional feeding
habit analysis indexes is removed, and the ecological interpretation capacity is enhanced. Compared with an environment
DNA (eDNA) technology, the method directly reflects the
ingestion behavior, and avoids false positive of eDNA,
database dependency defects and the like; according to the method, the
living body sample treatment process is optimized, the influence of an anatomical digestive
system on natural bait
pollution and the like is eliminated, meanwhile, the composition of the natural bait of the
nematode fish can be comprehensively reflected, the method has the advantages of being rapid, accurate, easy and convenient to operate and the like, a new technical method is provided for
stream fish
ecology research, and the method is worthy of application and popularization.