The invention discloses a lactoalbumin antihypertensive
peptide and a preparation method and an application thereof. The lactoalbumin antihypertensive
peptide is prepared by the following steps: after hydrating lactoalbumin solution, adjusting the pH value to 7.0-10.0, adding
protease I for enzymolysis reaction; after reacting for 120-240 minutes, keeping
reaction temperature constant; adjusting the pH value to 6.0-8.0; adding
protease II into a
reaction system to continue reacting; after reacting for 120-240 minutes, stopping reacting to obtain rough
enzyme solution; inactivating
enzyme in the rough
enzyme solution, and adjusting the pH value to the
isoelectric point of lactoalbumin; centrifuging to obtain supernate; adjusting the pH value of the supernate to 6.0-8.0; and collecting ultrafiltrate by a 10 kDa
ultrafiltration membrane; and obtaining lactoalbumin antihypertensive
peptide.
Product analysis indicates that the prepared lactoalbumin antihypertensive peptide contains less than 2% of ash content,
protein recovery rate approaches to 90%, TCA
soluble nitrogen content is more than 85%,
free amino acid content is 70.23 mu mol / mL, and ACE suppression ratio is more than 85%.