The invention discloses a
virus circulating batch culture device and a culture method, and particularly relates to the technical field of
virus culture. The device comprises a
fixed bed reactor, a circulating tank, a water bath interlayer, a
chip carrying
bed, an upper basket, a lower basket, a liquid
flow divider and a water bath jacket, wherein a water bath jacket is arranged on the outer side of the circulating tank so as to control the temperature in the circulating tank; liquid in the circulating tank enters the
fixed bed reactor for reaction; the
fixed bed reactor comprises an upper basket at the upper part and a lower basket at the lower part, and the middle part is a
chip loading
bed for culturing viruses; a water bath interlayer is arranged on the outer side of the fixed
bed reactor and used for controlling the temperature in the fixed bed reactor, and cultured viruses are collected through a liquid
flow divider. According to the fixed bed reactor, the
shear force and stress borne by cells are small,
suspension culture components such as a defoaming agent do not need to be added into a culture medium,
perfusion can be achieved without
cell interception, the upper limit of the
perfusion flow speed is high, continuous cells are friendly, and
virus production can be amplified by dozens of times or dozens of times.