120 results about "Fraction Collector" patented technology

The invention relates to liquid chromatography techniques for rapidly characterizing samples, such as polymer solutions, emulsions and dispersions, and to devices for implementing such techniques. The system includes a design that provides the ability to perform chromatographic separations by using mobile phase composition gradients and temperature gradients during separations. The invention accomplishes this by providing mixing zones for a plurality of fluids as well as heated and chilled feeds feeding the fluids to the mixing zones. Additionally, the system provides the ability to analyze separated components with a detector and/or collect separated fractions into vessels of a fraction collector for further separation and/or analysis.

Systems and methods for analyzing a plurality of samples in parallel include a plurality of liquid phase separation regions, a plurality of microfluidic storage regions, and a common mass spectrometer. Samples are separated in parallel in the separation regions to yield a plurality of output streams that are stored in the storage regions. The contents of each storage region, or at least a representative portion thereof, are sequentially discharged, ionized, and directed to the inlet of a mass spectrometer. In this manner, multiple separations are conducted in parallel with outputs provided serially to a common mass spectrometer without any loss of data. Microfluidic storage regions minimize diffusion between bands of separated samples. Portions of separated samples may be directed to fraction collectors.

Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable in interchangeable manner at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform recognizes the modules and their locations, coordinates fluid connections between the modules, and provides a variety of control, monitoring, data generating and data processing functions to generate chromatographic data.

The invention discloses a method for preparing ethyl eicosapentaenate (EPA) and ethyl docosahexaenoate (DHA) and relates omega-3 long-chain polyunsaturated fatty acids. The method comprises: preparing prepared solution to be separated by using a fish oil coarse raw material, which contains ethyl EPA and ethyl DHA, and using an organic solvent as a solvent; transferring the prepared solution to be separated into a material storage tube of a semi-prepared/prepared high-efficiency liquid chromatography-mass spectrum combined apparatus system, starting the semi-prepared/prepared high-efficiency liquid chromatography-mass spectrum combined apparatus system of which a mobile phase is aqueous solution of methanol or ethanol, balancing a chromatographic column, sampling automatically, separating and purifying ethyl EPA and ethyl DHA, and trigging a distillate collector by a mass spectrum online detection signal to collect pure ethyl EPA liquid and ethyl DHA liquid separately and automatically; and subjecting the pure ethyl EPA and ethyl DHA to reduced pressure concentration, extraction and reduced-pressure concentration respectively and thus obtaining ethyl EPA and ethyl DHA, of which the purities are both over 99 percent. The method is characterized by simple and convenient operation and high yield and is suitable for automatic control and synchronous separation.

A sample is introduced from an injector unit (3) into a mobile phase. One target component is separated into a plurality of vials (67) by a fraction collector (6). Next, a sampler (34) sequentially suctions the eluate from the plurality of the vials (67) and performs an LC analysis on each of the eluate portions, thereby producing a chromatogram. A peak detector (72) calculates the peak area corresponding to the amount of the target component in each chromatogram. A delay estimator (73) extracts the peak area in the fraction having the maximum peak area and the peak areas in the previous and succeeding fractions on the time axis of the foregoing fraction. Then, the delay estimator (73) estimates a delay volume from a detector (5) to the tip end of the dispenser nozzle (72) based on data such as the peak areas, the flow rate of the liquid fed from the pump (2), positions of the vials (67), and the position of the peak of the target component in the chromatogram detected by the detector (5), and stores the delay volume. Upon preparative separation of a desired component, a delay time is calculated based on the delay volume and the flow rate and the timing for the preparative separation is controlled based on the delay time.

The invention relates to a separating preparative chromatography apparatus comprising a serial preparative chromatography column, which mainly comprises a BV-3 two-position three-way electromagnetic valve, a BV-6 two-position six-way electromagnetic valve, a BV-4 two-position four-way electromagnetic valve, a C1 first-stage chromatographic separation column, a C21 second-stage chromatographic separation column 1, a C22 second-stage chromatographic separation column 2, a C23 second-stage chromatographic separation column 3, a C2n second-stage chromatographic separation column n, an MI manual injector, a PC pre-column, a PA infusion pump and a PB infusion pump, a detector and a fraction collector. The C1 first-stage chromatographic separation column is continuously utilized for separating fractions F1-Fn, a group of C21, C22, C23, ...... C2n are respectively injected into the second-stage chromatographic separation columns for separating and purifying compound monomers; the first-stage chromatographic separation column is serially connected with the second-stage chromatographic separation columns, and the second-stage chromatographic separation columns are connected in parallel for independently separating and purifying the compound monomers; and programs of the BV-3 two-position three-way electromagnetic valve, the BV-6 two-position six-way electromagnetic valve and the BV-4 two-position four-way electromagnetic valve are controlled by a chromatographic working station.

The invention provides a clinical urine sample fraction collector, which belongs to the field of medical equipment, and comprises a front segment collector and a middle segment collector, wherein a first and a second collecting pipes are detachably and vertically arranged on the top ends of the front and the middle segment collectors, the first and the second collecting pipes are communicated through a first overflow pipe which is arranged in the transverse direction, a hopper is arranged on the top portion of the first collecting pipe, a first and a second floating plugs are respectively placed in the front and the middle segment collectors, bottom openings of the first and the second collecting pipes are respectively plugged under the action of buoyancy of the first and the second floating plugs, and an overfall port is arranged on the side wall of the bottom end of the second collecting pipe. The clinical urine sample fraction collector can separate and collect front segment urine and middle segment urine at the same time, and overcomes the defects in the prior art that urine collectors just can collect front segment urine or mixed urine, thereby meeting demands of different diagnosis.

The invention is used in various branches of industry, in particular in agriculture for preparing seeds for sowing and for selection purposes. The method of separating a granular mixture in a flowing medium is based on a gravitational supply of particles, with an aerodynamic, monotonously increasing action thereon with a cascade of flat jets, said cascade being stabilized in terms of pressure and discharge. Large impurities are removed from the granular mixture beforehand, and stable parameters are maintained in the separation chamber. The device comprises a vibrating screen, an adjustable hopper with an agitator and a vibrating chute, a generator of a cascade of flat jets, which is connected to a drive for supplying air under pressure, a fraction collector and a separation chamber. The output of the separation chamber is covered with a filtering element in the form of a rotating drum with a calibrating sifter on the surface, said sifter being connected to a cyclone.

The invention discloses a preparation method of fucoxanthin and relates to the fucoxanthin. The preparation method of the fucoxanthin comprises the steps that a cleaned brown algae material is aired, and soaked by an organic solvent for extraction; an obtained extracting solution is condensed, and a fucoxanthin crude extract is obtained; the fucoxanthin crude extract is subjected to column chromatography separation; a fraction of the fucoxanthin is collected and condensed, and a fucoxanthin raw material is obtained; the obtained fucoxanthin raw material is dissolved by the organic solvent and transferred to a sample introduction bottle of a semipreparative/preparative HPLC (high performance liquid chromatograph); the semipreparative/preparative HPLC is started for separation and purification; fucoxanthin purification liquid is collected automatically through ultraviolet on-line detection or by a fraction collector triggered by a mass spectrum signal; and after the collected fucoxanthin purification liquid is decompressed, condensed, frozen and dried, the high-purity fucoxanthin is obtained. Through testing, the purity of the prepared fucoxanthin is greater than 99%.

The invention provides a multi-dimensional liquid chromatography separation system, comprising a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid dilution pump, a gradient mixer A, a gradient mixer B, a sampling valve, an enriching column array A, an enriching column array B, a fraction collector, a liquid chromatographic separation column array, a detector, a two-position ten-way valve and a connecting pipeline, wherein the two-position ten-way valve switches to realize the transformation between the last-dimensional separation state and the next-dimensional separation state, so as to realize three or more-dimensional chromatographic separation. Respective-dimensional chromatographic separation columns are selected through the liquid chromatography separation column array, thereby, based on the same gradient elution system and the same detector, realizing the full-line monitoring and control ofthe multi-dimensional chromatographic separation, and realizing the controllability of cleanliness degree of the enrichment column and the separation column. Respective-dimensional separations are connected by the enrichment column, and the liquid dilution pump is used to assist in compound enrichment or capture. The system achieves efficient separation of monomer compounds in complex system samples with high separation difficulty by selecting different combinations of chromatographic stationary phases and mobile phases.

The invention discloses a method for separating and preparing anti-tumor components ganoderic acid C1 and ganoderic acid F, which comprises the following steps: (1) primarily enriching and purifying the alcohol extract of lucid ganoderma by use of macroporous resin, and separating a ganoderic acid part with purity of 30-60% from the alcohol extract of lucid ganoderma; (2) separating the separated ganoderic acid part through high-speed counter-current chromatography to obtain ganoderic acid with relatively high purity, wherein a solvent system consists of petroleum ether, ethyl acetate, methanol and water at a volume ratio of 5:(3-7):(1-6):(4-9); the upper phase of the solvent is a stationary phase, and the lower phase is a mobile phase; a sample is dissolved with the lower phase solution and fed; the flow velocity of the stationary phase is 10-20mL/min, the flow velocity of the mobile phase is 1.5-3mL/min, and the rotation speed of the host is 600-1,000rpm; the separated object is collected by an automatic fraction collector, and one fraction is collected from every 4-10mL; (3) analyzing and detecting the components of the liquid collected by each test tube by high performance liquid chromatography, collecting, concentrating and drying according to different components, and separating to obtain ganoderic acid C1 and ganoderic acid F with purity up to 95%. The method has the characteristics of high efficiency, high speed, large separation quantity, high recovery rate, solvent saving and the like.

A method in a fraction collector for accurate positioning of a dispensing device with respect to wells of a micro plate in which prior to the dispensing a sensing device is moved over the micro plate the sensing device being able to detect the number of walls between the wells in the rows and columns of the micro plate.

The invention relates to a preparation method of fucoxanthol. The preparation method comprises the following steps: under the situation of adding a catalyst, enzymolyzing fucoxanthin by adopting lipase, reacting, generating fucoxanthol, concentrating and drying, extracting through methanol and concentrating to obtain a crude fucoxanthol extract; enabling the crude fucoxanthol extract to be used as a solution medium in a form of a mobile phase, configuring into preparation liquid to be separated, transferring the preparation liquid to be separated into a semi-preparative/preparative efficient liquid phase chromatography sampling bottle and starting a semi-preparative/preparative efficient liquid phase chromatography, wherein the mobile phase is selected from one of a methanol-water solution, an ethanol-water solution or an acetonitrile-water solution; balancing a chromatographic column, automatically sampling, separating and purifying the fucoxanthol, triggering a fraction collector through ultraviolet on-line detection and in combination with mass spectrometry detection signals, automatically collecting fucoxanthol purification liquid, and decompressing, condensing, freezing and drying the purification liquid to obtain the fucoxanthol of which the purity is larger than 99 percent. The preparation method is simple in operation, high in yield, suitable for automatic control and preparation of the high-purity fucoxanthol.

A method for fractionating a sample solution includes the steps of setting a first mass spectrometry condition and mass range information; setting a second mass spectrometry condition and mass range information; executing a mass scan by the mass spectrum acquisition portion under the first mass spectrometry condition and obtaining first mass spectrum data; extracting first chromatogram data from the first mass spectrum data based on the first mass range information; executing a mass scan by the mass spectrum acquisition portion under the second mass spectrometry condition to obtain second mass spectrum data; extracting second chromatogram data from the second mass spectrum data based on the second mass range information; switching the first and second spectrometry conditions and repeating the mass scan cyclically; adding the first and second chromatograph data to obtain a chromatogram data; and operating the fraction collector based on the chromatogram data.

The invention provides a multi-dimensional liquid chromatography separation system. The system comprises a high-performance liquid chromatography gradient pump A, a high-efficiency liquid chromatography gradient pump B, a high-performance liquid chromatography diluting pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a double-two-position four-way valve and a connecting pipeline; and switching between a previous one-dimensional separation state and a next one-dimensional separation state is realized through switching of the double-two-position four-way valve, and chromatography separation of three dimensions or above is realized. A chromatography separation column of each dimension is selected through the liquid chromatography separation column array; and on the basis of the same gradient elution system and the same detector, full online monitoring and control of multi-dimensional chromatography separation are realized, and the cleaning degree of enrichment columns and separation columns is controllable. The separationof all dimensions is connected through the enrichment columns, and the diluting pump is used for assisting in enrichment or trapping of compounds. Different chromatography stationary phase and mobilephase combinations are selected, so that efficient separation of monomeric compounds in a complex system sample with high separation difficulty is realized.

The invention relates to the field of mineral processing and can be used for separating solid particles according to density, for example in factories in the polymetal industry, in tin production plants and in gold mining plants, both at the initial enrichment stage and in the processing of industrial waste. The technical result consists in simplifying the design and increasing the operational reliability of the apparatus as well as enhancing the quality of the concentrate produced. The concentrator comprises a rotor (1), mounted on a rotational shaft, a feed pipe (2), a light fraction receiver (3) and a heavy fraction collector (4). The rotor (1) comprises a cone (5) with a base, and a cylindrical shell (6) with an annular runoff lip (7) on the light fraction receiver side and an annular lip for collecting the heavy fraction (8). An annular gap (9) is provided between the edge of the large base of the cone and the cylindrical shell (6). The inner cone of the rotor is rigidly connected to the cylindrical shell. A sleeve (11) with a seal having a stationary tube (12) inserted therein is disposed along the rotor axis on the base of the inner cone. The sleeve (11) is connected to channels oriented inside the shell (6) for supplying flushing water to the outer shell.

The invention discloses a non-target screening analysis method for intoxicating organic pollutants in wastewater based on a molecular network. The method comprises the following steps of: a) carryingout solid-phase extraction on pretreated wastewater to obtain an organic pollutant concentrated solution; b) fractionating the organic pollutant concentrated solution by using a high performance liquid chromatograph equipped with an automatic fraction collector to obtain a to-be-detected fraction; c) analyzing and detecting the to-be-detected fraction in the step b) by adopting a high performanceliquid chromatography-time-of-flight mass spectrometer; and d) processing the mass spectrum data detected in the step c) by using open source analysis software, generating a molecular network associated with the organic pollutants, and analyzing substances of network nodes. According to the non-target screening analysis method for the intoxicating organic pollutants in the wastewater based on themolecular network, the pollutant molecular network is generated, the network nodes display the response intensity of the substance in different components in a pie chart form, and the concentrations of the same substance in different components can be visually compared.

The invention provides a multi-dimensional liquid chromatography separation system. The system comprises a high-performance liquid chromatography gradient pump A, a high-efficiency liquid chromatography gradient pump B, a high-performance liquid chromatography diluting pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position eight-way valve and a connecting pipeline; and conversion between a previous one-dimensional separation state and a next one-dimensional separation state is realized through switching of the two-position eight-way valve, and chromatographic separation of three dimensions or above is realized. A chromatographic separation column of each dimension is selected through the liquid chromatography separation column array; and on the basis of the same gradient elution system and the same detector, fullonline monitoring and control of multi-dimensional chromatography separation are realized, and the cleaning degree of enrichment columns and separation columns is controllable. The separation of all dimensions is connected through the enrichment columns, and the diluting pump is used for assisting in enrichment or trapping of compounds. Different chromatography stationary phase and mobile phase combinations are selected, so that efficient separation of monomeric compounds in a complex system sample with high separation difficulty is realized.