28 results about "Fraction Collector" patented technology

The utility model provides an automatic sample injection-fraction collection-automatic sample application integrated system, and belongs to the technical field of liquid chromatography. According to the utility model, the sample injection function of the automatic sample injector, the fraction collection function of the fraction collector and the automatic sample application function of the automatic sample application instrument are integrated, sample injection, collection and sample application parameters are set through the control system, the moving mechanism is controlled to drive the sample injection needle to move to required positions according to different stages, and the sample injection function of the sample injector is realized by arranging the two-position three-way valve and the two-position six-way valve. And the complete process of sample introduction, fraction collection and sample application is completed in sequence by switching pipelines of the control system. According to the utility model, automatic sample injection can be realized, after liquid chromatography separation and detection, effluent is collected to a specified collection bottle as required, and after collection, automatic sample application of each fraction on the thin-layer plate is realized.

The invention discloses a multi-column parallel automatic chromatographic system, which is provided with more than two chromatographic columns connected in parallel, and all the chromatographic columns share a mobile phase device, a detection device and other devices, and has the following advantages: (1) the chromatographic columns connected in parallel share a set of software control system, so that the simultaneous operation of the plurality of chromatographic columns is realized; the product analysis, separation and purification time is shortened, and the labor cost is reduced; (2) the chromatographic columns connected in parallel share a mobile phase device, a detector device, a fraction collector and other devices, and equipment is reduced; and (3) common parts are used, so that the plant area is reduced, the load-bearing requirement is reduced, and the load-bearing and area requirements of the plant are reduced, thereby reducing the construction cost.

The utility model relates to the technical field of chromatography, in particular to a fraction collector which comprises a collector body, and the collector body comprises a container containing area, a gas inlet and a gas outlet. A dispensing head located above the container placement area; the moving assembly is used for driving the distribution head to move in the horizontal direction; the collector comprises a plurality of collector bodies, and the plurality of collector bodies are stacked in the vertical direction, so that the plurality of container placing areas are formed in the vertical direction. Therefore, the containers can be placed in the container placing areas with different heights and do not need to be placed in the same horizontal plane, so that the occupied area of the fraction collector can be reduced when the same number of containers are accommodated.

The invention discloses a double-column circulating chromatographic system which comprises an isocratic elution device, a gradient elution device, a pre-column valve, a chromatographic column device, a post-column valve, a detector device, a waste liquid tank, a fraction collection device, a reflux device and a connecting pipeline, the isocratic elution device and the gradient elution device are connected with the pre-column valve, the chromatographic column device and the post-column valve through connecting pipelines after being connected in parallel, the post-column valve is connected with the detector device and the waste liquid tank which are arranged in parallel through connecting pipelines, and the detector device is connected with the fraction collector through a connecting pipeline. The method has the beneficial effects that (1) not only can the purification efficiency and yield be improved, but also stable peak appearance time and a highly consistent purification spectrum can be obtained, so that stable impurity spectrum distribution is obtained, and continuous automatic production is realized; and (2) multi-time multi-system automatic circulation purification of the same system is realized, the purification efficiency is remarkably improved, human intervention and operation errors are reduced, the pollution risk is reduced, and industrialization of continuous flow purification is realized.

1. The name of the design product: fraction collector. 2. The use of the design product: the design product is used for fraction collector. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view 1.

A preparative chromatograph system includes: a chromatograph part including a separation column and a detector; a fraction collector for executing a fractionation collecting operation of dropping and collecting the eluate flowed out from the separation column through a nozzle into a plurality of collection containers; a controller configured to control an operation of the fraction collector based on a preset fractionation collecting condition; an information processing device configured to perform information processing relating to the fractionation collecting operation; and a display. The information processing device is configured to output a chromatogram created based on a signal of the detector to the display in a state where fractionated sections on the chromatogram collected in the plurality of collection containers by the fraction collector and collection information including a collection purpose for which each fractionation section has been collected are added to the chromatogram.

The invention provides a method for chiral resolution of a cyflumetofen enantiomer. The cyflumetofen enantiomer in a cyflumetofen test solution is separated by adopting an ultrahigh liquid chromatography, a stationary phase is a polysaccharide derivative chiral chromatographic column, a mobile phase A is an organic solvent, a mobile phase B is deionized water, the mobile phase B is subjected to gradient elution according to a volume percentage ratio of 15-5% within 0-5 minutes, and the mobile phase B is subjected to gradient elution according to a volume percentage ratio of 15-5% within 5-10 minutes. The mobile phase B is subjected to isocratic elution according to the volume percent of 5%, the column temperature is 35 DEG C, and the flow velocity is 0.7 mL / min; a detector is adopted for determination, and cyflumetofen chiral monomer fractions are collected in a fraction collector. The invention provides the cyflumetofen chiral enantiomer which can be effectively separated and can be used for preparing a certain scale, the method overcomes the defects in the prior art, and the method has the characteristics of mild and simple operation conditions, high sensitivity, high separation speed, high purity of the separated monomer, green and environment-friendly mobile phase and the like.

The utility model discloses an automatic chromatographic system with multiple parallel columns, which is provided with more than two parallel chromatographic columns, and all the chromatographic columns share a mobile phase device, a detection device and other devices, and has the following advantages: (1) the parallel chromatographic columns share a set of software control system, so that the simultaneous operation of the multiple chromatographic columns is realized; the product analysis, separation and purification time is shortened, and the labor cost is reduced; (2) the chromatographic columns connected in parallel share a mobile phase device, a detector device, a fraction collector and other devices, and equipment is reduced; and (3) common parts are used, so that the plant area is reduced, the load-bearing requirement is reduced, and the load-bearing and area requirements of the plant are reduced, thereby reducing the construction cost.

A preparative chromatograph system includes: a chromatograph section having a separation column and a detector fluidly connected downstream of the separation column and outputting a signal based on a component concentration of an eluent from the separation column; a fraction collector, which has a plurality of collection containers, and which performs, downstream of the chromatograph unit, a separation operation in which fractions of the eluent from the separation column are dropped into the collection containers from the nozzles and collected; a controller that controls the fraction collector based on a fractionation condition; an information processing device; the information processing device is configured so as to associate all fractions collected in the collection container by the fraction collector with a chromatogram created on the basis of a signal from the detector, thereby dividing the chromatogram into fraction sections corresponding to the respective fractions collected in the collection container. On the basis of the separation conditions, separation result information is created and output to a display, said separation result information being obtained by adding collection information including the separation purposes of the fractions corresponding to all of the fraction sections.

The utility model discloses a fraction collector capable of preventing sample loss, which comprises an adjusting assembly, a control assembly fixedly connected to the adjusting assembly, a device shell fixedly connected to the adjusting assembly, a supporting assembly fixedly connected to the device shell, a collecting assembly detachably connected to the device shell, and a fixing buckle fixedly connected to the device shell. The control assembly comprises a special-shaped connecting block, a small air cylinder, a liquid outlet pipe, a connecting opening, a collected liquid buffer, a liquid inlet pipe and a rubber plug, the special-shaped connecting block is internally provided with a leak-proof reserved groove and a liquid circulation hole, and the fraction collector can control outflow and closing of fraction liquid samples, so that economic loss and resource waste are avoided, and the fraction collector is suitable for popularization and application. The adjusting assembly can conveniently and rapidly collect the fraction liquid sample and control the precision of the outflow position, and the fraction collector can be adjusted through the supporting assembly so that collection of the fraction liquid sample can be observed more visually.

1. Name of the designed product: Fraction collector. 2. Use of the designed product: For collecting fractions. 3. Design points of the designed product: In shape. 4. Picture or photo best indicating the design points: Fig. 1.

The invention relates to a method for measuring the dead volume of a fraction collector, which comprises the following steps of: respectively collecting and performing polynomial fitting by setting a plurality of set values in ranges on two sides of an estimated value, so as to calculate the optimal dead volume.

The invention discloses a method for separating kinsenoside in anoectochilus formosanus by high-speed counter-current chromatography, which comprises the following steps: firstly, extracting anoectochilus formosanus with methanol, dissolving the extract with pure water after the extract is dried by distillation under reduced pressure, extracting with chloroform, drying a water layer by distillation under reduced pressure to obtain a sample, then selecting a solvent system consisting of ethyl acetate, ethanol and water (15: 1: 15), taking the following phase as a stationary phase and the upper phase as a mobile phase, the method comprises the following steps: separating and purifying a sample by adopting a tail-to-head elution mode and using high-speed counter-current chromatography, collecting a micro fraction containing kinsenoside by using a BSZ-100 type fraction collector, finally detecting by using high performance liquid chromatography, and determining the purity of the micro fraction, according to the method, the kinsenoside in the anoectochilus formosanus can be separated in one step through high-speed counter-current chromatography, the separation purity of the kinsenoside is larger than 96%, aftertreatment is simpler, and efficient and rapid preparation of the kinsenoside is achieved.

A fraction collector (14) that is provided downstream of a detector (10) and for separating an eluate eluted from a separation column (8) into a plurality of parts and collecting each of the parts into a plurality of containers respectively, and a controller (16) configured to execute fractionation operation for each sample injected at one time into the mobile phase are provided. In the fractionation operation, the controller detects components separated from each other in the separation column (8) as peaks individually based on a signal output from the detector (10), and controls operation of the fraction collector (14) such that the components detected as the peaks are collected in different containers. The controller (16) is configured to set a reference number of the number of components in a sample injected into the mobile phase, to count the number of components detected as the peaks for each sample injected into the mobile phase at a time by the injector (6), and to determine that the fractionation operation of the sample is completed when the number of components counted reaches the reference number and all components as many as the reference number are collected in the container.

The present invention includes a support system (1, 2), a carrier (42) movably supported by the support system (1, 2), an extension arm (3) connected to the carrier (42), and at least one dispensing head (31) movably connected to the extension arm (3) for dispensing droplets, wherein the dispensing head (31) and the carrier (42) are configured to move relative to the support system in a first plane, a tray area (11) is designed to support at least one rack (6, 7), and the at least one rack (6, 7) is designed to hold at least one collection container (12, 14), and movement of the carrier (42) is achieved by a linear bearing Y-slide unit (41), the linear bearing Y-slide unit (41) is arranged below a linear bearing Y-rail (40), and the carrier (42) is attached to the linear bearing Y-slide unit (41).

The utility model discloses a circulating device for high-speed counter-current chromatography. The circulating device comprises a plurality of control valves, a plurality of capture rings, a mobile phase storage tank, a pump, a fraction collector, a counter-current chromatography host, a detector and a chromatography workstation, the mobile phase storage tank is communicated with the pump pipeline; the pump is communicated with the counter-current chromatography host through a pipeline; the counter-current chromatography host is communicated with the detector through a pipeline; the detector is connected with the chromatographic workstation computer; circulating control valves are arranged on a pipeline for communicating the mobile phase storage tank with the pump and a pipeline for communicating the capturing ring with the fraction collector; and the capture rings are respectively communicated with the detector and the fraction collector through capture control valves through opening and closing pipelines. By arranging a plurality of capture rings connected in parallel and correspondingly arranged state switching control valves, various substances with similar solubility / partition coefficients and isomer substances contained in natural products can be circulated so as to achieve effective separation, and the purity of difficult-to-separate substances obtained after the substances are separated by the device can reach 98% or above.

A transmission device is arranged on a mounting frame, a liquid dropping device is movably connected to the transmission device, a collecting frame is arranged on the mounting frame, a splash-proof device is arranged in a first placing plate of the collecting frame, a collecting device is arranged in a second placing plate of the collecting frame, and the bottom of the splash-proof device is inserted into the collecting device. The collecting device comprises a primary collecting pipe and a secondary collecting pipe, and a final collecting device is arranged below the collecting device; according to the utility model, the splash-proof device is arranged, so that the receiving area of fraction liquid is effectively increased, the possibility that the liquid splashes outwards is reduced, and meanwhile, the enclosure is arranged to surround the whole collecting area, so that the liquid is further prevented from splashing to the outside; by arranging the primary collecting pipe, the secondary collecting pipe and the final collecting device, multi-stage protection is set for preventing the fraction liquid from flowing out, even if the upper collecting pipe overflows excessively, the fraction liquid can be collected by the lower collecting device, equipment is prevented from being polluted by accumulated liquid, and meanwhile the loss of the fraction liquid is reduced.

To enable a user to easily recognize the purpose of each fraction collected in a collection container on the basis of preparation result information.SOLUTION: A preparative chromatograph system includes a separation column 16, a chromatograph part 2 having a detector 18, a fraction collector 4 having a collection container 24, a controller 6 configured so as to control operation of the fraction collector 4, an information processing device 8 configured so as to perform information processing about preparation operation of the fraction collector 4 and a display 10 for displaying information outputted from the information processing device 8. The information processing device 8 divides chromatograms into fraction sections that respectively correspond to all fractions collected in the collection container 24 by associating all fractions collected in the fraction collector 4 with created chromatograms, and creates preparation result information obtained by attaching respective preparation purposes of fractions corresponding to all fraction sections on the basis of a preparation condition to output the preparation result information to the display 10.SELECTED DRAWING: Figure 1

The utility model relates to the field of environmental monitoring and analytical chemistry, in particular to a high-concentration matrix eliminating device which comprises an automatic sample injector, a first reagent bottle for storing nitric acid, a second reagent bottle for storing ammonium acetate, a third reagent bottle for storing ultrapure water, a three-way pipe, an enriching column, a six-way valve and an automatic fraction collector, the first reagent bottle, the second reagent bottle and the third reagent bottle are sequentially connected with a first connector through pipelines, the first connector is used for switching and selecting passages, the outlet end of the first connector is connected with a first plunger pump through a selector valve, and six connecting points are arranged in the six-way valve. A first connecting point of the six-way valve is connected with the first plunger pump, the three-way pipe is provided with three connecting ends, a second connecting point of the six-way valve is connected with a first connecting end of the three-way pipe, and a sample injection needle of the automatic sample injector is connected with the second connector through a pipeline. The utility model solves the problem that a target analyte exists in a high-concentration matrix and is difficult to detect.

The invention relates to a fraction collector apparatus 100 comprising: a support system 1, 2, a carrier 42 moveably supported by the support system 1, 2, an extension arm 3 connected to the carrier 42, at least one dispensing head 31 for dispensing droplets and moveably connected to the extension arm 3, wherein the dispensing head 31 and carrier 42 are configured to move relative to the support system in a first plane, a tray area 11 designed to support at least one rack 6, 7, wherein the at least one rack 6, 7 is designed to hold at least one collection vessel 12, 14, wherein motion of said carrier 42 is achieved by a linear bearing Y-slide unit 41 wherein the linear bearing Y-slide unit 41 is arranged underneath a linear bearing Y-rail 40 and wherein the carrier 42 is attached to the linear bearing Y-slide unit 41.

A preparative liquid chromatograph for separating and extracting components in a sample, the preparative liquid chromatograph including a separation channel (2) through which a mobile phase flows, an injector (6) that injects the sample into the mobile phase, a separation column (8) for separating components in the sample injected into the mobile phase, the separation column (8) being provided downstream of the injector (6) on the separation channel (2), a detector (10) for detecting peaks of the components separated by the separation column (8), a fraction collector (12) configured to collect an eluate from the separation column (8) into a plurality of collection containers (20:22) at a downstream of the detector (10) while fractionating the eluate, and a controller (14) for controlling operation of the fraction collector (12), wherein the fraction collector (12) is provided with peak collection containers (20) and waste liquid collection containers (22), each peak collection container (20) is used for collecting a peak portion including a collection target peak detected by the detector (10) in the eluate, and each waste liquid collection container (22) is used for collecting a waste liquid portion not including the collection target peak in the eluate, and the controller (14) is configured to collect the peak portion including the collection target peak detected by the detector (10) in the eluate in the peak collection container (20), and to collect at least a part of the waste liquid portion in the waste liquid collection container (22).

A separation channel on which a liquid delivery pump, a separation column, and a detector are provided, the liquid delivering pump being for feeding a mobile phase, the separation column being for separating a component in a sample, and the detector being for detecting peaks of components separated from each other by the separation column, a plurality of liquid handlers each including an injecting part and a collecting part, wherein when one of the plurality of liquid handlers is introduced into the separation channel, the injecting part of the one of the plurality of liquid handlers functions as an injector that executes injection operation of a sample into a mobile phase flowing through the analysis channel upstream of the separation column in the analysis channel and the collecting part of the one the plurality of liquid handlers functions as a fraction collector that executes collection operation of peaks of components separated from each other in the separation column, a selector for selectively switching a liquid handler to be introduced into the separation channel among a plurality of the liquid handlers, and a controller for controlling operation of a plurality of the liquid handlers and the selector.

This invention provides a preparative liquid chromatograph that can also handle situations where the actual second delay time changes after the start of sequence separation. During sequence separation, after a specific component is detected as a peak in either the first or second detector, the control device performs a maintenance operation on the time difference information stored in the information storage area. In this maintenance operation, the control device performs a peak determination to determine whether the difference between the first and second retention times is within a specified allowable range, thereby confirming whether the second delay time from the detection of the component in the second detector to the arrival of the component in the fraction collector has changed compared to the previous state. If the control device determines, through peak determination, that the second delay time has changed compared to the previous state, it updates the time difference information stored in the information storage area to the latest state using the calculated difference between the first and second retention times.

The utility model discloses a double-column circulating chromatographic system which comprises an isocratic elution device, a gradient elution device, a pre-column valve, a chromatographic column device, a post-column valve, a detector device, a waste liquid tank, a fraction collecting device, a reflux device and a connecting pipeline, the isocratic elution device and the gradient elution device are connected with the pre-column valve, the chromatographic column device and the post-column valve through connecting pipelines after being connected in parallel, the post-column valve is connected with the detector device and the waste liquid tank which are arranged in parallel through connecting pipelines, and the detector device is connected with the fraction collector through a connecting pipeline. The device disclosed by the utility model has the beneficial effects that (1) not only can the purification efficiency and the yield be improved, but also stable peak appearance time and a highly consistent purification spectrum can be obtained, stable impurity spectrum distribution can be obtained, and automatic production can be realized; and (2) multi-time multi-system automatic circulation purification of the same system is realized, the purification efficiency is remarkably improved, human intervention and operation errors are reduced, the pollution risk is reduced, and industrialization of continuous flow purification is realized.