172 results about "Fucoxanthin" patented technology

The present invention provides a composition for a medicinal or health effect of a treatment of liver fat and body fat, a reduction of blood pressure, an increase of the energy expenditure rate, a reduction of inflammatory C-reactive proteins and a reduction of plasma aminotransferase enzymes, comprising an effective amount of fucoxanthin alone or in combination with pomegranate seed oil, a pharmaceutically acceptable salt, a prodrug thereof, or a salt of the prodrug; and a method of using the same. The fucoxanthin may be used in pure form, or as a component of a brown marine vegetable extract.

The invention relates to a method for extracting fucoxanthin from brown algae, which is characterized by comprising the following steps: pulping fresh brown algae, adding an organic reagent into the brown algae, and then adding 0.05 to 0.1 mass permillage of antioxidant into the brown algae; leaching the mixture for 22 to 26 hours, and performing suction filtration to obtain leaching solution; leaching the mixture repeatedly until an organic layer is colorless; combining the organic layer liquid of each time into a dark brown bottle; performing rotary evaporation on the organic layer liquid to remove an organic solvent; adding ethyl acetate and water according to a volume ratio of 1:1-2 into an adherence material obtained in a rotary evaporation utensil, oscillating the mixture for layering after dissolving the adherence material, collecting the organic layer, and repeating the operations for 3 to 6 times; combining each organic layer liquid and then performing rotary evaporation and concentration to obtain a total pigment of the brown algae; and separating and purifying the total pigment by using silica gel column chromatography to obtain the high-purity fucoxanthin. The fucoxanthin prepared by the method has high purity, and the method has simple and practical operation, is convenient for industrial production, and provides a basic condition for further development of correlative industrial products and medicines and health care products aiming at the market vacancy of the high-purity fucoxanthin at home and abroad.

The present invention relates to the extraction and separation of fucoxanthin, and is especially the process of separating fucoxanthin from algae. The process of separating fucoxanthin from algae includes the following steps: cleaning fresh algae or defrozen algae to eliminate surface salt ion, eliminating surface water, leaching algae with dimethyl sulfoxide in 2-6 ml for each gram of algae in dark for 15-60 min, and final extracting coloring matter from dimethyl sulfoxide leached liquid with the mixture solution of ethyl acetate and ammonium sulfate. The present invention has the advantages of simple operation, high separation speed and high yield, and is suitable for industrial production.

The invention discloses a method for extracting high-purity fucoxanthin from seaweeds. The method is characterized by comprising the following steps: cleaning the seaweeds by using the distilled water so as to remove saline ions on the surfaces of the seaweeds, and then removing moisture on the surfaces of the seaweeds; then adding an organic reagent to the seaweeds, digesting in the dark for 8-24 hours, and carrying out suction filtration so as to obtain a leaching liquid; repeatedly digesting the leaching liquid till the leaching liquid is colorless, and combining each leaching liquid into a brown bottle; finally carrying out rotary evaporation for a period of time till black green precipitates occur in a liquid, and filtering so as to remove pigment and impurities; and continuously carrying out rotary evaporation till red solids are separated out in the liquid, and filtering so as to obtain the red solids which are the high-purity fucoxanthin. The method has the advantages that the safely healthy high-purity fucoxanthin raw material is obtained for the first time under the condition that no chromatographic techniques are used for separation, and the preparation process is safe, harmfulless, economic and environment-friendly.

The invention provides a method for preparing algae fucosan and fucoxanthin in an enzymic way. The method specifically comprises the following steps of: preparing brown algae dry powder; processing the brown algae dry powder by enzymolysis: uniformly mixing the brown algae dry powder with a complex enzyme preparation, then adding deionized water to perform enzymolysis; performing acid treatment; acquiring concentrated liquor; and acquiring fucosan: acquiring crude fucoxanthin, and then acquiring pure fucoxanthin. The complex enzyme is composed of pectinase, hemicellulase and cellulose. The brown algae fucosan and the fucoxanthin prepared by the method are high in yield. The method is simple in operation, environment-friendly, efficient, and suitable for being used for synchronously extracting high-purity fucosan and fucoxanthin from brown algae with massive operation.

The invention discloses a method for comprehensively extracting fucoxanthin and brown alga polyphenol from gulfweed, which comprises the following steps: carrying out repeated freeze-thawing and centrifugation on fresh gulfweed or frozen reserved gulfweed to control the water content of brown alga; adding an organic reagent into the seaweed, leaching in a dark place for 1-24 hours, carrying out vacuum filtration to obtain a leaching solution, repeating the leaching operation until the leaching solution is colorless, merging the leaching solutions, concentrating the leaching solution until abundant precipitate appears in the solution, and filtering to remove the precipitate and obtain the filtrate; crystallizing the filtrate at low temperature, and filtering to obtain fucoxanthin; and adding a fat-soluble solvent into the filtrate, carrying out repeated liquid-liquid extraction until the fat-soluble solvent layer is colorless, merging the fat-soluble solvent layers of multiple extraction processes, and carrying out low-temperature concentration until a greenish yellow solid precipitates in the solution, thereby obtaining the brown alga polyphenol extract. The method has the advantages of high raw material utilization ratio and high extraction efficiency, and is simple to operate.

The invention relates to a method for extracting and purifying fucoxanthin from brown alga, which comprises the steps of primary extraction sample preparation, methanol dissolution, column chromatography separation and HPLC (high performance liquid chromatography) purification. The invention provides a fucoxanthin preparation scheme which has the advantages of high safety, low toxicity, high economy and environmental protection. The invention adopts kieselguhr as a column filler to separate the fucoxanthin for the first time. The kieselguhr has the characteristics of low price and environmental protection. Meanwhile, edible petroleum ether is utilized for separation, thereby lowering the residues of toxic substances in the final product. After the kieselguhr is separated, the fucoxanthin health product raw material which is cheap, safe and healthy can be obtained. The fucoxanthin is separated and purified by HPLC to obtain the chromatographically pure fucoxanthin. The invention provides the safe low-toxicity separation technique for satisfying the high-purity fucoxanthin market at home and abroad, greatly enhances the added value of the brown alga aquatic products, and thus, has important economic value.

The invention relates to extraction and separation of fucoxanthin, and in particular to a method for extracting and separating fucoxanthin from marine unicellular diatom. A fat-soluble extract is added into and mixed with the marine unicellular diatom mud, the mixture is extracted under the conditions of low temperature, darkness and no oxidation for 2-10 min, supernatant extract is collected by centrifugation, the precipitate is repeatedly extracted again by an extract, and the supernatants are merged and separated by liquid chromatography, so as to obtain high-purity fucoxanthin, wherein the usage amount of the extract is 4-6ml for per gram of algae mud. The invention has the advantages of simple operation, fast separation and high yield of fucoxanthin, lays the foundation for the factory production, and ultimately can be used for large-scale industrialized production.

The invention discloses a preparation method of fucoxanthin and relates to the fucoxanthin. The preparation method of the fucoxanthin comprises the steps that a cleaned brown algae material is aired, and soaked by an organic solvent for extraction; an obtained extracting solution is condensed, and a fucoxanthin crude extract is obtained; the fucoxanthin crude extract is subjected to column chromatography separation; a fraction of the fucoxanthin is collected and condensed, and a fucoxanthin raw material is obtained; the obtained fucoxanthin raw material is dissolved by the organic solvent and transferred to a sample introduction bottle of a semipreparative/preparative HPLC (high performance liquid chromatograph); the semipreparative/preparative HPLC is started for separation and purification; fucoxanthin purification liquid is collected automatically through ultraviolet on-line detection or by a fraction collector triggered by a mass spectrum signal; and after the collected fucoxanthin purification liquid is decompressed, condensed, frozen and dried, the high-purity fucoxanthin is obtained. Through testing, the purity of the prepared fucoxanthin is greater than 99%.

Compounds having the structure of general formula I or pharmaceutically acceptable salts thereof which are derivatives of fucoxanthin and fucoxanthol are disclosed. A preparation method of the compound and its use in weight reducing are also disclosed.

The invention discloses a method for extracting and purifying fucoxanthin. According to the method, a microwave assisted extraction technology is utilized, and ethanol serves as a solvent, so that fucoxanthin in brown seaweed raw materials can be rapidly extracted; and a liquid-liquid extraction and column chromatography two-step separation process is adopted, so that the fucoxanthin can be effectively separated from chlorophyll and other impurity components. The fucoxanthin is low in toxicity, safe and environment-friendly, ethanol is taken as an extraction solvent, and the use amount of a toxic organic solvent is effectively reduced; and moreover, a microwave extraction method is adopted, extraction can be finished within 5 minutes only, and the extraction time is greatly shortened. The method disclosed by the invention is simple, rapid and high-efficiency in the whole extraction and purification process, the operation is convenient, large-scale production is easily realized, and a foundation is laid for development and utilization of natural health food additives such as the fucoxanthin with extremely high values and biological drug resources.

The invention discloses a pretreatment method of brown alga samples for fucoaxanthin extraction. The pretreatment method is characterized by concretely including the steps that step1, the fresh brown algae are frozen for 1-30 days at the temperature of below 5 DEG C-80 DEG C and then taken out, air thawing is performed at room temperature until the center temperature of the brown algae reaches 0 DEG C-2 DEG C, and then the freeze thawing process is completed; the brown algae are placed into a centrifugal machine and centrifuged for 3-10 minutes at the rotation speed of 800 rpm-10000 rpm, and then water is removed; step2, the step1 is repeated 1-4 times, and a solvent extraction and precipitation method is adopted for preparing high-purity fucoxanthin from the brown algae after freeze thawing treatment is performed. The pretreatment method has the advantages that the fucoxanthin and other effective components in the brown algae can be effectively prevented from being damaged and decomposed, the difference between the water content of the surfaces of the brown alga samples and the water content inside the brown alga samples is small, inside and outside distribution is even, and the water content of the treated brown algae is 50-90%.

A method comprising a series of selective extraction techniques for the parallel production of biodiesel and isolation of several valuable co-products including an alkenone hydrocarbon mixture of the kerosene/jet fuel range (primarily C10-, C12-, and C17-hydrocarbons) and fucoxanthin, a high-valued carotenoid, from the marine alkenone-producing microalgae Isochrysis.

The invention relates to a preparation method of fucoxanthol. The preparation method comprises the following steps: under the situation of adding a catalyst, enzymolyzing fucoxanthin by adopting lipase, reacting, generating fucoxanthol, concentrating and drying, extracting through methanol and concentrating to obtain a crude fucoxanthol extract; enabling the crude fucoxanthol extract to be used as a solution medium in a form of a mobile phase, configuring into preparation liquid to be separated, transferring the preparation liquid to be separated into a semi-preparative/preparative efficient liquid phase chromatography sampling bottle and starting a semi-preparative/preparative efficient liquid phase chromatography, wherein the mobile phase is selected from one of a methanol-water solution, an ethanol-water solution or an acetonitrile-water solution; balancing a chromatographic column, automatically sampling, separating and purifying the fucoxanthol, triggering a fraction collector through ultraviolet on-line detection and in combination with mass spectrometry detection signals, automatically collecting fucoxanthol purification liquid, and decompressing, condensing, freezing and drying the purification liquid to obtain the fucoxanthol of which the purity is larger than 99 percent. The preparation method is simple in operation, high in yield, suitable for automatic control and preparation of the high-purity fucoxanthol.

The invention belongs to culture of microorganisms, in particular to a method for producing fucoxanthin through light culture of nitzchia laevis. The method comprises the following steps: preparing a seed solution; performing fermentation culture; extracting the fucoxanthin from fermentation liquor obtained after fermentation. By adopting the method, the problems of low biomass concentration, low fucoxanthin yield, low fucoxanthin content and the like of the nitzchia laevis under a phototrophic condition in the prior art are solved. Prepared nitzchia laevis dry powder has high fucoxanthin content and high fucoxanthin yield, the fucoxanthin produced by the method is safer than fucoxanthin derived from a large-sized seaweed source, the production efficiency is increased, the production cost is lowered, and meanwhile the pollution risk in a culturing process is lowered greatly.

The invention relates to the field of aquaculture and discloses a preparation method of a nonreactive aquatic product immunity enhancer. The preparation method comprises the following steps: (1) uniformly mixing 80 to 120 parts of aquatic product polypeptide, 40 to 60 parts of camellia seed meal modified extract, 10 to 30 parts of astaxanthin, 10 to 30 parts of fucoxanthin, 10 to 20 parts of pearl powder, 40 to 80 parts of seaweed extract and 300 to 500 parts of water to obtain a component A; (2) dissolving 20 to 30 parts of calcium chloride into 500 to 700 parts of water to obtain a component B; (3) dropwise adding the component A into the component B at the speed of 0.5mL/s to 1.5mL/s to produce solid sediment; filtering, taking the solid sediment and drying to obtain the nonreactive aquatic product immunity enhancer. The nonreactive aquatic product immunity enhancer provided by the invention is prepared from pure natural green raw materials and does not contain antibiotic drugs; the immunity of aquaculture animals can be effectively improved by the nonreactive aquatic product immunity enhancer, and the nonreactive aquatic product immunity enhancer does not cause pollution to the breeding environment and is green and environment-friendly.

The invention relates to a health-care food containing natural fucoxanthin, which is a soft capsule. Moreover, an obesity preventing model method is adopted, and the fucoxanthin is used as a functional factor for carrying out a fat reducing experiment on a rat. By the display of data analysis, the weight and the perinephric spermary fat weight of the rat fed with the fucoxanthin are obviously lowered in comparison with those of a model set. Furthermore, the fat cell area is obviously reduced in comparison with that of the model set and a blank set. The result shows that the fucoxanthin has a very good fat reducing effect, and a favorable basis is provided to the application of substances containing the fucoxanthin in the field of health-care foods.

The present invention relates to a technology for extracting high purity fucoxanthin from algae. The technology comprises steps of pretreatment of algae, solvent extraction of the fucoxanthin, and purification of the fucoxanthin. The technology of the invention solves the problems of low product purity and solvent residues of the extracted fucoxanthin in the prior art. Because it is easy to get raw materials employed in the technology of the invention, the extracted solvent is recoverable, the extraction yield is high, purification materials can be re-used, and the extraction and purification processes are simple, the technology of the invention has low cost and high efficiency and lays the foundation for the industrial production of fucoxanthin related products.

The invention discloses a fucoxanthin microcapsule powder and a preparation method thereof. The fucoxanthin microcapsule powder comprises water and non-water raw materials, wherein the mass of the water is 0.1-9 times of the total mass of the non-water raw materials; the non-water raw materials comprise the following components in percentage by weight: 0.1-10% of fucoxanthin, 0.5-90% of wall materials, 30-60% of an oil phase, 0.001-99% of a filling agent, 0.001-5% of an anticaking agent, 0.001-5% of edible essence, 0.001-5% of an aqueous phase antioxidant, 0.001-5% of an aqueous phase stabilizing agent and 0.001-5% of an oil phase emulsifying agent. The fucoxanthin microcapsule powder disclosed by the invention is high in fluidity and high in stability; after the fucoxanthin microcapsule powder melts in water, the fucoxanthin microcapsule powder can be stably stored for a long term, and the phenomena that oil floats, precipitate is generated, and the fucoxanthin microcapsule powder is stuck to bottles cannot appear. In the production process of the fucoxanthin microcapsule powder disclosed by the invention, waste water, waste residues and waste gas are not produced, the production technology is environment-friendly, and the fucoxanthin microcapsule powder is suitable for industrialized popularization.

The invention belongs to culture of microorganisms, and particularly relates to a method for producing fucoxanthin by heterotrophic culture of smooth nitzschia. The method comprises the following steps: preparation of a seed solution, fermental cultivation, extraction of the fucoxanthin from fermentation liquor after fermentation is finished and the like. The problem that the biomass concentration of the smooth nitzschia, the yield of the fucoxanthin and the content of the fucoxanthin are low under the condition of heterotrophic culture in the prior art is solved. The method has the advantages that the content of fucoxanthin in prepared dry smooth nitzschia powder is high, the yield of the fucoxanthin is high, the fucoxanthin is safer than fucoxanthin from large seaweeds, and while the production efficiency is improved and the production cost is reduced, pollution risks in a culture process is greatly reduced.