71 results about "Fucoxanthin" patented technology

The invention discloses a nucleic acid aptamer OUC-Fx11-5T of fucoxanthin and application of the nucleic acid aptamer OUC-Fx11-5T, and belongs to the technical field of nucleic acid aptamers. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ ID NO. 19. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ The invention further discloses the application of the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthin in identification or detection of the fucoxanthin. According to the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine, the affinity and dissociation constant of the nucleic acid aptamer OUC-Fx11-5T and the fucoxanthine is determined to be 68.2 nM through an isothermal titer thermal method, the nucleic acid aptamer OUC-Fx11-5T has no obvious affinity to structural analogues of the fucoxanthine such as violaxanthin, neoxanthin, zeaxanthin and beta-carotene, has high affinity and good specificity to the fucoxanthine, and can be used for preparing the fucoxanthine aptamer OUC-Fx11-5T of the fucoxanthine. The method can be used for identification or detection of fucoxanthine.

The invention discloses an antibacterial film loaded with a fucoxanthin nano material and a preparation method of the antibacterial film. The antibacterial film is prepared by taking a nitrogen-rich hollow carbon material-fucoxanthin (NHC-FX) nano material as an antibacterial photosensitizer, chitosan as a substrate of a film and glycerol as a plasticizer of the film through a solution casting method. The antibacterial film disclosed by the invention is good in hydrophobicity and high in stability, can reduce transmission of ultraviolet light, and has a good photodynamic antibacterial effect. The antibacterial food packaging material can be used as an antibacterial food packaging material or a preservative film, can prevent food from being polluted by microorganisms, and can isolate damage of ultraviolet rays to the food.

The invention discloses a preparation method and application of a targeted delivery system of a metal polyphenol nano system. The invention discloses a targeted delivery system of a metal polyphenol nano system, and a preparation method of the targeted delivery system comprises the following steps: taking the metal polyphenol nano system formed based on epigallocatechin gallate and iron ions as a carrier, and loading fucoxanthin to prepare fucoxanthin nano particles with active oxygen response characteristics; then, after chondroitin sulfate capable of targeting macrophages and (5-carboxyl amyl) triphenyl phosphorus bromide capable of targeting mitochondria are coupled, the fucoxanthin delivery system with the active oxygen response characteristic and the targeting characteristic is further prepared through a double emulsion anti-solvent evaporation method. The system can realize release of fucoxanthin under stimulation of active oxygen, and realizes aggregation of a targeted delivery system in mitochondria of a specific inflammation site. The carrying system can effectively improve or relieve the inflammatory bowel disease, and the raw materials used in the carrying system are low in cost, the preparation conditions are mild, and large-scale production is easy.

The invention discloses a culture method for enhancing the body color of Dongmaotai, and relates to the technical field of aquaculture. The method comprises the following steps: (1) illumination induction: alternately irradiating by adopting a light source white-red-blue-yellow, (2) feeding with a toner: feeding with basal feed at the same time, and (3) water flow stimulation: regulating physiological metabolism, nutrition absorption and environmental adaptability to realize the purpose of improving the quality of the feed by regulating the physiological metabolism, nutrition absorption and environmental adaptability. The method is beneficial to enhancing the body color of the east astroplatia and improving the economic value of the east astroplatia.

The invention belongs to the technical field of natural products, and provides application of fucoxanthin in preparation of oral anti-alopecia drugs. Animal experiments find that long-term high-fat diet can lead to large-piece shedding of hair accompanied by skin inflammation, and fucoxanthin has a treatment effect on high-fat diet induced mouse back hair shedding and skin inflammation, can obviously relieve high-fat diet induced subcutaneous cholesterol crystal accumulation and sebaceous gland cell hypertrophy, and has the effects of preventing and treating the high-fat diet induced mouse back hair shedding and skin inflammation and preventing and treating the high-fat diet induced mouse back hair shedding and the high-fat diet induced sebaceous gland cell hypertrophy. And skin epidermal hyperplasia and cuticle thickening induced by high fat diet can be improved. Besides, cell experiments also find that fucoxanthin intervention can reduce oleic acid infiltration of the hair follicle stem cells and reduce oleic acid induced death and apoptosis of the hair follicle stem cells, and prove that fucoxanthin has a protection effect on the hair follicle stem cells, has low toxic and side effects on cells and animals as a natural algae extract, and has a good application prospect. Good clinical application prospects are realized.

The invention discloses a saccharomyces cerevisiae strain for de novo synthesis of fucoxanthine precursor neoxanthine and application of the saccharomyces cerevisiae strain, and belongs to the technical field of biology. According to the recombinant saccharomyces cerevisiae disclosed by the invention, the 3-hydroxy-3-methylglutaryl coenzyme A reductase, the isopentenyl pyrophosphate isomerase, the farnesyl pyrophosphate synthase and the endoplasmic reticulum size regulation factor are expressed in an enhanced manner; according to the method, geranyl geranyl diphosphate synthase, phytoene dehydrogenase, 15-cis-phytoene synthase, bifunctional lycopene cyclase / phytoene synthase, beta-carotene hydroxylase, zeaxanthin epoxidase truncated mutant and violaxanthin deep oxidase-like protein are subjected to heterologous expression, so that the zeaxanthin deep oxidase-like protein is obtained; the ROX1 and GAL80 genes in the saccharomyces cerevisiae are knocked out, and the expression of squalene synthase ERG9 is down-regulated. When the strain is subjected to shake flask fermentation, the contents of neoxanthine and violaxanthin of the strain respectively reach 1.78 mg / L and 26.77 mg / L, so that the strain has a wide application prospect.

The invention belongs to the technical field of biology, and particularly relates to a method for increasing the fucoxanthine accumulation amount by using microcystis subjected to depolymerization treatment to feed palmarthria formosana. According to the method, microcystis subjected to depolymerization treatment are added in the culture process of the palmitothecium for heterotrophic culture, meanwhile, continuous inflation is conducted, the microcystis subjected to depolymerization treatment are swallowed by the palmitothecium, and the higher fucoxanthine accumulation amount in the palmitothecium is obtained by means of the culture mode. The experiment proves that after the microcystis is subjected to pretreatment and depolymerization, the removal efficiency of the microcystis is higher by the brown algae, the limitation of treatment of harmful cyanobacteria and ingestion of protozoa is made up, and meanwhile, an integrated solution is provided for biological treatment of cyanobacterial bloom and resource utilization of the microalgae; meanwhile, a new way is provided for obtaining fucoxanthine with higher accumulation amount.

The application discloses a fucoxanthin-maltodextrin delivery system based on ultrasonic-assisted encapsulation and a preparation method thereof. The delivery system comprises a maltodextrin derivative obtained by hydrophobic modification of maltodextrin with octanoyl chloride and fucoxanthin encapsulated therein. The preparation method of the fucoxanthin-maltodextrin delivery system based on ultrasonic-assisted encapsulation disclosed in the application can graft octanoyl chloride onto a maltodextrin chain through esterification to prepare an amphiphilic maltodextrin derivative which can self-aggregate into aggregates. The ultrasonic-assisted encapsulation can load more fucoxanthin and target the delivery of the fucoxanthin to the intestinal tract. The method has the advantages of short encapsulation time, high efficiency of embedding and delivery of hydrophobic substances, etc. The maltodextrin aggregates prepared by the method have high efficiency of encapsulating and delivering fucoxanthin, have a more compact structure, a smaller particle size and a larger specific surface area, so that the maltodextrin aggregates have a higher drug loading capacity. The maltodextrin derivative prepared has acid stability and can be released in the intestinal tract in a targeted manner, and has great application value in the transportation of nutrients of food and the design of functional food.

The invention relates to the field of microalgae active component extraction, and discloses a method for extracting fucoxanthine and polyunsaturated fatty acid from microalgae and an extracted product thereof, the method comprises the following steps: S1, carrying out first extraction on the microalgae by using a low-concentration alcohol solution, and carrying out solid-liquid separation to obtain a first algae cake and a first extracting solution; and S2, carrying out second extraction on the first algae cake obtained in the step S1 by using a high-concentration alcohol solution, and carrying out solid-liquid separation to obtain a second algae cake and a second extracting solution. According to the method, the low-concentration alcoholic solution and the high-concentration alcoholic solution are matched for extraction, the method is simple, efficient, low in cost and suitable for large-scale production, the problem of residues of toxic solvents such as n-hexane is avoided, the alcoholic solution can be recycled, the proportion of the low-concentration alcoholic solution and the high-concentration alcoholic solution in a final finished product is close to that in natural microalgae cells, and the method is suitable for large-scale production. And the composition does not need to be compounded again.

The application discloses a nucleic acid aptamer OUC-Fx11-5T of fucoxanthin and application thereof, and belongs to the technical field of nucleic acid aptamer. The nucleic acid aptamer OUC-Fx11-5T of fucoxanthin has a nucleotide sequence as shown in SEQ ID NO. 19. The nucleic acid aptamer OUC-Fx11-5T of fucoxanthin is applied to the recognition or detection of fucoxanthin. The nucleic acid aptamer OUC-Fx11-5T of fucoxanthin has an affinity dissociation constant of 68.2 nM with fucoxanthin by isothermal titration calorimetry, has no obvious affinity to structural analogs of fucoxanthin such as violaxanthin, neoxanthin, zeaxanthin and beta-carotene, has high affinity and good specificity to fucoxanthin, and can be used for the recognition or detection of fucoxanthin.

The invention belongs to the technical field of health-care food and traditional Chinese medicine preparation, and discloses a medicinal and edible drink for treating prostatitis and a preparation method thereof.The drink is prepared by taking houttuynia cordata, dandelion, purslane and endive as monarch drugs, matching lalang grass rhizome, reed stem root, talc and yam rhizome as ministerial drugs, assisting saffron, and supplementing astragalus membranaceus, Chinese yam, fructus lycii and oyster; and meanwhile, lycopene and fucoidan are added. The preparation method comprises the following steps: pretreatment of raw material cleaning, drying and grouped crushing; carrying out low-temperature cold extraction by adopting water or an ethanol-water mixed solvent at 0-10 DEG C; the cold extraction liquid is heated to 80-95 DEG C and kept for 10-30 min, and high-temperature glowing is completed; the obtained liquid is sequentially subjected to rough filtration, fine filtration and vacuum concentration, instant high-temperature sterilization is conducted under the condition of 121 DEG C, and finally a liquid beverage, a concentrated solution or instant powder is prepared. Through combination of a reasonable formula and a specific technology, stable extraction and retention of various effective components are achieved, and the product quality consistency is good.

The present invention discloses a heat shock transcription factor for increasing the content of fucoxanthin in Phaeodactylum tricornutum bd1650 gene and its application, characterized in that bd1650 the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein encoded by bd1650 is as shown in SEQ ID NO: 2. By knocking out the bd1650 gene in Phaeodactylum tricornutum, the content of fucoxanthin synthesized by it is increased. The advantage is that the content of fucoxanthin in Phaeodactylum tricornutum can be significantly increased.

The present application relates to a kind of by chrysophyte fermentation-light coupling high-efficiency production of fucoxanthin method, comprising: chrysophyte is inoculated into fermenter and is carried out heterotrophic culture, while the algal liquid is continuously or intermittently lighted;Culture medium includes basic medium and feed medium, C / N is 4:1-40:1, nitrogen source uses organic nitrogen source or inorganic ammonium nitrogen;The glucose concentration in basic medium is 5-15g / L, temperature is 25-32℃, dissolved oxygen is controlled at 5-80%, pH is 4-8, light intensity 5-600 μmol m ‑2 s ‑1 ;Glucose concentration is monitored in time during the cultivation process, when glucose concentration is reduced to 0.5-3g / L, using peristaltic pump to flow feed medium is added to flow culture, control the glucose concentration in whole flow culture process in 1-10g / L;After 100h of cultivation, every 1-5h is sampled to detect the biomass dry weight contained in the culture medium in fermenter, when the detection value is unchanged or begins to drop, fermentation is finished, harvest, and the harvested product is used for extracting fucoxanthin.The present application establishes the feasibility basis of fermentation-light coupling chrysophyte cultivation to scale production of fucoxanthin.

The invention discloses an integrated extraction device for fucoxanthine, and relates to the technical field of extraction equipment. Due to the fact that materials on the bottom layer and materials on the upper layer cannot be exchanged in the heating and stirring process of solvents and materials in an extraction container, heating is uneven, and the extraction effect is affected, according to the fucoxanthine integrated extraction device, due to the fact that the zigzag appearance of a bottom scraper is matched with an inner guide plate, in the process of stirring and shoveling the materials at the bottom of a tank body, the materials on the bottom layer cannot be exchanged; the zigzag appearance of the bottom scraper is used for driving a solvent and a material, centrifugal force in a rotating state is matched with the inner guide plate, so that the material and the volume of the bottom layer move towards the outer side, and the side scraper and the outer guide plate are matched, so that the internal material and the solvent circularly flow in the tank body, the heating uniformity is improved, and the extracted fucoxanthine is uniformly fused into the solvent.

The invention discloses a heat shock transcription factor for increasing the content of fucoxanthin in Phaeodactylum tricornutum 49557 Genes and their applications are characterized by 49557 The nucleotide sequence of the gene is shown in SEQ ID NO: 3, and the amino acid sequence of the protein encoded by 49557 is shown in SEQ ID NO: 4. 49557 The gene increases the content of fucoxanthin synthesized by it, which has the advantage of significantly increasing the content of fucoxanthin in Phaeochromis triangularis.

The application discloses a culture method for enhancing the body color of Eaststar Prawn and relates to the technical field of aquaculture. The method comprises the following steps: (1) light induction: white-red-blue-yellow light sources are alternately used for irradiation, (2) color enhancer feeding: the color enhancer is fed simultaneously with the basic feed, and the components of the color enhancer include Camellia nitidissima polysaccharide, Sargassum fusiforme polysaccharide, fucoxanthin, scallop peptide, silymarin, guanidino acetic acid and sodium butyrate, and (3) water flow stimulation. Through the regulation of physiological metabolism, nutrient absorption and environmental adaptability, the method jointly acts on the body color performance of Eaststar Prawn, is favorable for enhancing the body color of Eaststar Prawn and improving the economic value of Eaststar Prawn.

The present invention relates to a water-soluble fucoxanthin and a preparation method thereof. The water-soluble fucoxanthin is a water-soluble particle formed by self-assembly of fucoxanthin, fatty acids, bovine serum albumin, and water-soluble chitosan. The preparation method comprises the following steps: (1) preparing water-soluble chitosan; (2) preparing a fucoxanthin-fatty acid mixture; (3) preparing alkaline electrolyte water; (4) preparing a fucoxanthin-protein complex by electrolysis water coupling ultrasound technology; (5) preparing a chitosan-protein-fatty acid-fucoxanthin complex; and (6) obtaining water-soluble fucoxanthin. The present invention adopts alkaline electrolysis water coupling ultrasound technology and non-covalent self-assembly technology to prepare water-soluble fucoxanthin with good stability and high absorption rate. The preparation process is simple, the embedding rate is high, the safety is good, the production cost is low, the fucoxanthin is suitable for industrial production, and the fucoxanthin can be widely used in food systems as a nutritional enhancer.

The application belongs to the field of microalgae biotechnology, and particularly relates to a brownish chrysochromulina and a method for producing fucoxanthin by using the brownish chrysochromulina. The brownish chrysochromulina is named PCSM-1, and was preserved in China Center for Type Culture Collection on January 11, 2024, with a preservation number of CCTCC NO: M 2024076. The content of fucoxanthin in the brownish chrysochromulina is as high as 1.58 wt%, and the brownish chrysochromulina can grow under photoautotrophic conditions and heterotrophic conditions. The brownish chrysochromulina is first subjected to heterotrophic fermentation to rapidly proliferate and ferment, and then is subjected to induction under photoautotrophic and heterotrophic conditions to rapidly accumulate fucoxanthin. The whole process has a short cycle, low energy consumption, and a far higher yield of fucoxanthin than existing microalgae, and can solve the problems of low production efficiency and high cost of fucoxanthin.

The invention discloses an MIE3 gene for improving the fucoxanthin content in phaeodactylum tricornutum and application thereof, and is characterized in that the nucleotide sequence of the MIE3 gene is shown as SEQ ID NO.1, the amino acid sequence of protein coded by the MIE3 gene is shown as SEQ ID NO.2, the MIE3 gene in the phaeodactylum tricornutum is knocked out, so that the content of synthesized fucoxanthin is improved, and the content of fucoxanthin in the phaeodactylum tricornutum is increased. The sgRNA sequence adopted for knocking out the MIE3 gene in the phaeodactylum tricornutum is as follows, the nucleotide sequence of sgRNA1 is as shown in SEQ ID NO.5, and the nucleotide sequence of sgRNA2 is as shown in SEQ ID NO.6. The method has the advantages that the MIE3 is found and proved to be a key negative regulation factor for synthesis of the fucoxanthin, and the content of the fucoxanthin in the phaeodactylum tricornutum can be remarkably increased through gene modification.

The invention relates to a fucoidan composition and a preparation method thereof. The fucoidan composition comprises the following components in percentage by weight: 20-30% of nemacystus japonicus fucoidan, 20-30% of almacystus japonicus fucoidan, 20-30% of 25-35% of sporophyll carbohydrate gum of the undaria pinnatifida in the Tasmanni Australian sea area; 25-35% of fucus saccharide gum in the Australian Tasmanni subsea area; 5 to 10% of microalgae fucoidan; and 1-5% of microalgae fucoxanthin. According to the fucoidan composition disclosed by the invention, the effective inhibition rate of the prepared fucoidan composition on human colon cancer, human pancreatic cancer, human liver cancer, human prostatic cancer, human breast cancer or human lung cancer and the like reaches 89% or above through an optimized formula of multiple components.

The present invention discloses a method for preparing fucoxanthin-loaded pectin@β-cyclodextrin, comprising the following steps: (1) preparing a fucoxanthin stock solution; (2) preparing a PBS stock solution; and (3) preparing fucoxanthin-loaded pectin@β-cyclodextrin. The method for preparing fucoxanthin-loaded pectin@β-cyclodextrin of the present invention has simple process steps and strong operability. By loading fucoxanthin on pectin@β-cyclodextrin, the stability of fucoxanthin in vitro and in vivo is improved, so that fucoxanthin can better reach the target site and improve its bioavailability in the gastrointestinal tract. The present invention also provides an application of fucoxanthin-loaded pectin@β-cyclodextrin in lipid-lowering foods, which can provide ideas for the application and further development of fucoxanthin in foods.

Compositions with weight-loss effects and their applications belong to the field of biomedicine. To improve the fat-reducing effect and further solve the off-target effect problem caused by existing weight-loss mechanisms, the composition includes fucoxanthin 0.8%~2.5%, ginsenoside CK 0.6%~1.2%, black ginseng active peptide 0.3%~0.6%, amylase inhibitor 12%~30%, prickly pear cactus extract 6%~15%, allicin 0.2%~0.6%, and excipients. This invention achieves weight regulation through comprehensive synergy of multiple targets.

The invention discloses an industrial application method of smooth nitzschia in biosynthesis of environment-friendly fucoxanthin. A high-yield mutant strain is obtained by combining multiple rounds of ARTP mutagenesis with CRISPR / Cas9 gene editing, and an excellent strain is quickly locked by utilizing intelligent high-throughput screening. High-density culture and efficient synthesis are realized by combining multi-stage dynamic fermentation with metabolic flow regulation and control. The culture is optimized in the intelligent photobioreactor, the energy consumption is reduced, the cyclic utilization of waste liquid and algae residues is realized in cooperation with a green process, and the environmental pollution is reduced. And the high-purity fucoxanthin is extracted by combining supercritical CO2 extraction with a microfluid crushing technology. The content is obviously increased, the cost is reduced, and the method is environment-friendly, is suitable for large-scale production in food, medicine and feed industries, and has a wide market prospect.

The invention belongs to the field of medicines, foods and health-care products, and particularly relates to a compound with an ionizing radiation protection effect and application thereof. The effective component of the compound with the ionizing radiation protection effect is fucoxanthin or a pharmaceutically acceptable salt thereof. The compounds are used independently or jointly to be prepared into medicines, health foods, functional foods or food additives. The fucoxanthin is a natural compound, can be extracted from seaweed, does not depend on limited chemical raw materials and complex synthesis processes, has the advantages of safety, effectiveness, clear molecular mechanism and the like, and has potential advantages in safety and compliance in long-term use compared with current marketed drugs.

The application discloses a preparation method and application of food-derived cellulose-based nanocomposite particles, and belongs to the field of precise nutrition delivery. The preparation method of the food-derived cellulose-based nanocomposite particles comprises the following steps: preparing oxidized food-derived nanocellulose through a TEMPO oxidation method on the basis of food-derived cellulose; mixing a protein aqueous solution with the oxidized food-derived nanocellulose suspension; then adding an ethanol solution containing a hydrophobic active substance, homogenizing, and removing the ethanol to obtain the food-derived cellulose-based nanocomposite particles. The method increases the overall structural stability of the nanoparticles, improves the encapsulation efficiency of fucoxanthin and the stability of the fucoxanthin during storage, and the fucoxanthin encapsulated in the nanoparticles can be effectively absorbed by cells, significantly improves the bioavailability of the fucoxanthin, and effectively inhibits the formation of lipid droplets by activating the Nrf2 / HO-1 / NQO1 signal pathway to reduce oxidative stress.

The present invention relates to a vision protection functional food and its production process, which specifically comprises the following steps: (1) preparing fucoxanthin nanoparticles by alkaline electrolyzed water-ultrasonic coupling technology; (2) mixing the fucoxanthin nanoparticle powder with quercetin, β-sitosterol, docosahexaenoic acid, water or solid filler excipients in proportion to obtain an aqueous solution mixture or a solid mixture; (3) homogenizing and filtering the aqueous solution mixture; or sieving the solid mixture; (4) sterilizing and filling the filtered aqueous solution mixture, or tableting and granulating the sieved solid mixture, and preparing the mixture into products respectively. The present invention can significantly improve the antioxidant capacity of the eyes and retinal function, effectively prevent myopia and reduce the risk of retinal-related ophthalmic diseases; the product can be made into capsules, tablets, granules, oral liquids or beverages, has a simple production process, is low in cost, and is suitable for large-scale industrial production.

The invention relates to the technical field of natural product extraction, and discloses a fucoxanthin extraction, separation and solvent circulation method based on quaternary ammonium salt-metal salt composite ionic liquid, and the method comprises the following steps: S1, mixing a quaternary ammonium salt cation precursor and metal salt to obtain the composite ionic liquid; s2, mixing the algae powder with a compound ionic liquid, and performing ultrasonic stirring extraction to obtain an extraction mixture; s3, adding a salting-out agent into the extraction mixture, centrifuging and collecting an upper phase rich in fucoxanthin; s4, filtering the upper phase with diatomite or a short silica gel column, and performing vacuum dehydration; separating and purifying the composite ion phase subjected to vacuum dehydration to obtain high-purity fucoxanthin, and recycling the composite ion liquid. According to the scheme, fucoxanthin in algae is extracted with the quaternary ammonium salt-metal salt composite ionic liquid, links such as extraction, primary separation and solvent circulation are integrated, and compared with a traditional organic solvent method, the method has remarkable advantages in the aspects of selectivity, efficiency, product purity and environmental influence.

The invention discloses functional feed for litopenaeus vannamei, and belongs to the field of feed for the litopenaeus vannamei. According to the technical scheme, the functional feed for the litopenaeus vannamei consists of a basal feed and a brown algae source additive, the addition amount of the brown algae source additive in the basal feed is 1-2 g / kg; the brown algae source additive is prepared from fucoxanthin and sodium alginate; fucoxanthin and sodium alginate are extracted from the same brown algae in a co-production manner; the brown algae source additive is a microcapsule formed by wrapping fucoxanthin with sodium alginate as a wall material. In order to solve the technical problems that most of the existing prawn feed additives are from chemical synthesis sources and have certain limitations in the aspects of promoting growth and enhancing immunity, the invention provides the functional feed for the litopenaeus vannamei, which contains natural bioactive substances, has definite and controllable components and can promote the growth of the prawns and improve the immunity of the prawns.

The invention provides nitzschia glauca capable of resisting low temperature and application of the nitzschia glauca, and the provided nitzschia glauca has the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 20252152. According to the invention, the novel nitzschia oryzae strain with remarkable competitive advantages is successfully screened through a 300MeV proton heavy ion physical mutagenesis technology. The growth rate of the strain in a low-temperature environment of 10-15 DEG C is obviously superior to that of a wild type, and the problem of short outdoor culture cycle in high-latitude areas such as northeast is effectively solved; meanwhile, the mutant strain realizes double increase of biomass and high-value components, the fucoxanthin content of the mutant strain is remarkably increased at normal temperature, the lipid and protein content higher than those of a wild type can still be kept at low temperature, and the mutant strain shows extremely high broad-spectrum temperature adaptability and application economic value.

The invention discloses a preparation method of a sargassum fusiforme extract, and belongs to the technical field of sargassum fusiforme extraction and separation, and the preparation method comprises the following steps: S1, extracting dry sargassum fusiforme powder to obtain an extracting solution A and residues; s2, carrying out concentration and alcohol precipitation on the extracting solution A to respectively obtain a precipitate A and a supernatant A; s2.1, dissolving the precipitate A, enabling the precipitate A to pass through macroporous resin, and collecting effluent; and S2.2, concentrating the effluent, and treating to obtain a fucoxanthine product. S3, carrying out water extraction, concentration and alcohol precipitation on the residues to respectively obtain a precipitate C and a supernatant B; and S4, combining the supernate A and the supernate B, concentrating, passing through macroporous resin, concentrating and drying effluent to obtain a brown algae polyphenol product, and the like. According to the preparation method, fucoxanthine, fucoidan and brown algae polyphenol can be extracted at the same time, the recovery rate of the three active ingredients is high, and the product purity is high.