27 results about "Fucoxanthin" patented technology

The application discloses a fucoxanthin-maltodextrin delivery system based on ultrasonic-assisted encapsulation and a preparation method thereof. The delivery system comprises a maltodextrin derivative obtained by hydrophobic modification of maltodextrin with octanoyl chloride and fucoxanthin encapsulated therein. The preparation method of the fucoxanthin-maltodextrin delivery system based on ultrasonic-assisted encapsulation disclosed in the application can graft octanoyl chloride onto a maltodextrin chain through esterification to prepare an amphiphilic maltodextrin derivative which can self-aggregate into aggregates. The ultrasonic-assisted encapsulation can load more fucoxanthin and target the delivery of the fucoxanthin to the intestinal tract. The method has the advantages of short encapsulation time, high efficiency of embedding and delivery of hydrophobic substances, etc. The maltodextrin aggregates prepared by the method have high efficiency of encapsulating and delivering fucoxanthin, have a more compact structure, a smaller particle size and a larger specific surface area, so that the maltodextrin aggregates have a higher drug loading capacity. The maltodextrin derivative prepared has acid stability and can be released in the intestinal tract in a targeted manner, and has great application value in the transportation of nutrients of food and the design of functional food.

The present application relates to a kind of by chrysophyte fermentation-light coupling high-efficiency production of fucoxanthin method, comprising: chrysophyte is inoculated into fermenter and is carried out heterotrophic culture, while the algal liquid is continuously or intermittently lighted;Culture medium includes basic medium and feed medium, C / N is 4:1-40:1, nitrogen source uses organic nitrogen source or inorganic ammonium nitrogen;The glucose concentration in basic medium is 5-15g / L, temperature is 25-32℃, dissolved oxygen is controlled at 5-80%, pH is 4-8, light intensity 5-600 μmol m ‑2 s ‑1 ;Glucose concentration is monitored in time during the cultivation process, when glucose concentration is reduced to 0.5-3g / L, using peristaltic pump to flow feed medium is added to flow culture, control the glucose concentration in whole flow culture process in 1-10g / L;After 100h of cultivation, every 1-5h is sampled to detect the biomass dry weight contained in the culture medium in fermenter, when the detection value is unchanged or begins to drop, fermentation is finished, harvest, and the harvested product is used for extracting fucoxanthin.The present application establishes the feasibility basis of fermentation-light coupling chrysophyte cultivation to scale production of fucoxanthin.

The application discloses a culture method for enhancing the body color of Eaststar Prawn and relates to the technical field of aquaculture. The method comprises the following steps: (1) light induction: white-red-blue-yellow light sources are alternately used for irradiation, (2) color enhancer feeding: the color enhancer is fed simultaneously with the basic feed, and the components of the color enhancer include Camellia nitidissima polysaccharide, Sargassum fusiforme polysaccharide, fucoxanthin, scallop peptide, silymarin, guanidino acetic acid and sodium butyrate, and (3) water flow stimulation. Through the regulation of physiological metabolism, nutrient absorption and environmental adaptability, the method jointly acts on the body color performance of Eaststar Prawn, is favorable for enhancing the body color of Eaststar Prawn and improving the economic value of Eaststar Prawn.

The application belongs to the field of microalgae biotechnology, and particularly relates to a brownish chrysochromulina and a method for producing fucoxanthin by using the brownish chrysochromulina. The brownish chrysochromulina is named PCSM-1, and was preserved in China Center for Type Culture Collection on January 11, 2024, with a preservation number of CCTCC NO: M 2024076. The content of fucoxanthin in the brownish chrysochromulina is as high as 1.58 wt%, and the brownish chrysochromulina can grow under photoautotrophic conditions and heterotrophic conditions. The brownish chrysochromulina is first subjected to heterotrophic fermentation to rapidly proliferate and ferment, and then is subjected to induction under photoautotrophic and heterotrophic conditions to rapidly accumulate fucoxanthin. The whole process has a short cycle, low energy consumption, and a far higher yield of fucoxanthin than existing microalgae, and can solve the problems of low production efficiency and high cost of fucoxanthin.

The invention discloses an MIE3 gene for improving the fucoxanthin content in phaeodactylum tricornutum and application thereof, and is characterized in that the nucleotide sequence of the MIE3 gene is shown as SEQ ID NO.1, the amino acid sequence of protein coded by the MIE3 gene is shown as SEQ ID NO.2, the MIE3 gene in the phaeodactylum tricornutum is knocked out, so that the content of synthesized fucoxanthin is improved, and the content of fucoxanthin in the phaeodactylum tricornutum is increased. The sgRNA sequence adopted for knocking out the MIE3 gene in the phaeodactylum tricornutum is as follows, the nucleotide sequence of sgRNA1 is as shown in SEQ ID NO.5, and the nucleotide sequence of sgRNA2 is as shown in SEQ ID NO.6. The method has the advantages that the MIE3 is found and proved to be a key negative regulation factor for synthesis of the fucoxanthin, and the content of the fucoxanthin in the phaeodactylum tricornutum can be remarkably increased through gene modification.

Compositions with weight-loss effects and their applications belong to the field of biomedicine. To improve the fat-reducing effect and further solve the off-target effect problem caused by existing weight-loss mechanisms, the composition includes fucoxanthin 0.8%~2.5%, ginsenoside CK 0.6%~1.2%, black ginseng active peptide 0.3%~0.6%, amylase inhibitor 12%~30%, prickly pear cactus extract 6%~15%, allicin 0.2%~0.6%, and excipients. This invention achieves weight regulation through comprehensive synergy of multiple targets.

The invention relates to the technical field of natural product extraction, and discloses a fucoxanthin extraction, separation and solvent circulation method based on quaternary ammonium salt-metal salt composite ionic liquid, and the method comprises the following steps: S1, mixing a quaternary ammonium salt cation precursor and metal salt to obtain the composite ionic liquid; s2, mixing the algae powder with a compound ionic liquid, and performing ultrasonic stirring extraction to obtain an extraction mixture; s3, adding a salting-out agent into the extraction mixture, centrifuging and collecting an upper phase rich in fucoxanthin; s4, filtering the upper phase with diatomite or a short silica gel column, and performing vacuum dehydration; separating and purifying the composite ion phase subjected to vacuum dehydration to obtain high-purity fucoxanthin, and recycling the composite ion liquid. According to the scheme, fucoxanthin in algae is extracted with the quaternary ammonium salt-metal salt composite ionic liquid, links such as extraction, primary separation and solvent circulation are integrated, and compared with a traditional organic solvent method, the method has remarkable advantages in the aspects of selectivity, efficiency, product purity and environmental influence.

The invention provides nitzschia glauca capable of resisting low temperature and application of the nitzschia glauca, and the provided nitzschia glauca has the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 20252152. According to the invention, the novel nitzschia oryzae strain with remarkable competitive advantages is successfully screened through a 300MeV proton heavy ion physical mutagenesis technology. The growth rate of the strain in a low-temperature environment of 10-15 DEG C is obviously superior to that of a wild type, and the problem of short outdoor culture cycle in high-latitude areas such as northeast is effectively solved; meanwhile, the mutant strain realizes double increase of biomass and high-value components, the fucoxanthin content of the mutant strain is remarkably increased at normal temperature, the lipid and protein content higher than those of a wild type can still be kept at low temperature, and the mutant strain shows extremely high broad-spectrum temperature adaptability and application economic value.

Provided is a technology capable of improving antimicrobial properties in a fucoxanthin-containing composition. A method for producing a fucoxanthin-containing composition includes the step of culturing a diatom in a medium at pH 1.0 or more and pH 4.0 or less, wherein the diatom has 18S rRNA composed of the following (a) or (b): (a) a polynucleotide consisting of a nucleotide sequence represented by SEQ ID NO: 1; or (b) a polynucleotide consisting of a nucleotide sequence having 85% or more identity to the nucleotide sequence represented by SEQ ID NO: 1.

The invention pertains to a nutritional composition comprising xanthohumol, fucoxanthin and c- phycocyanin. Further, the invention pertains to a nutritional composition comprising xanthohumol, fucoxanthin and c-phycocyanin for use as a medicament, more particularly for therapeutic use in treating and / or preventing muscle wasting.

The present application belongs to the field of biotechnology, and particularly relates to a method for improving accumulation of fucoxanthin, oleic acid and arachidonic acid in brown flagellate culture. The method is to add microcystis to brown flagellate for mixed culture, and continuously aerate, and the microcystis is phagocytosed by the brown flagellate. The method of phagocytosis of microcystis by brown flagellate is used to realize higher accumulation of fucoxanthin in brown flagellate. Through the experiment, it is proved that the brown flagellate has higher removal efficiency for non-toxic microcystis, and can also effectively ingest toxic microcystis, and a new way for obtaining higher accumulation of fucoxanthin in biological manufacturing is provided.

The invention belongs to the technical field of chemical materials, and particularly relates to fucoxanthin-coated lipidosome as well as a preparation method and application thereof. The fucoxanthin-coated liposome provided by the invention comprises the following components in percentage by weight: 30-50% of water; 30 to 50 percent of polyhydric alcohol; 5-10% of an antioxidant; 1-5% of a solubilizer; 1% to 5% of isosorbide dimethyl ether; 1-5% of lecithin; 1-5% of a dispersant; 0.1-1% of a stabilizer; and the fucoxanthin accounts for 0.05 to 5 percent. The fucoxanthin liposome has the advantages that by optimizing the components of the fucoxanthin liposome, the permeability of the fucoxanthin on the skin surface is enhanced, and the skin is prevented from being stained; meanwhile, the fucoxanthin-coated liposome provided by the invention has very good water solution solubility, can be directly added into a water phase, is small in skin irritation and high in stability, and has a wide practical application value.

The invention relates to the technical field of biological environment engineering, and provides a fucoxanthin synthesis optimization method and system based on reinforcement learning, and the method comprises the following steps: a real-time data acquisition system; constructing a state space; a deep reinforcement learning decision network; optimizing a dynamic strategy; and strategy verification is linked with the process. Through a light quality-stimulant collaborative reinforcement learning regulation strategy, the static operation limitation of a traditional process is broken through, the photosynthetic physiological state of microalgae is perceived in real time, the light environment and chemical signals are dynamically optimized, the fucoxanthin synthesis efficiency is remarkably improved, and a multi-omics closed-loop verification mechanism is combined, so that the fucoxanthin synthesis efficiency is improved. The molecular cascade effect of a light signal driving pigment synthesis pathway is illuminated, and a precise target spot is provided for metabolic engineering transformation.

This invention discloses a cryopreservation diluent containing fucoxanthin and its applications. Adding fucoxant to the cryopreservation diluent enhances the antioxidant capacity of frozen-thawed semen, reduces oxidative damage to goat sperm from reactive oxygen species during freezing-thawing, significantly improves sperm viability, motility, plasma membrane integrity, and total antioxidant capacity after freezing-thawing, enhances catalase and total superoxide dismutase activity, and reduces reactive oxygen species and malondialdehyde content. The effect is best when the fucoxant concentration is 2 μM during cryopreservation. The cryopreservation diluent of this invention is simple to prepare and operate, significantly improves the cryopreservation effect of semen, and has important practical application value.

The invention relates to a high-fucoxanthine algae cell culture method, which realizes rapid accumulation of fucoxanthine by adjusting the nitrogen-carbon ratio and starvation culture transition in the microalgae growth process and the fucoxanthine accumulation process, and finally obtains microalgae cells with high fucoxanthine content.

This invention provides a composition that improves the permeability of fucoxanthin into cell tissue. [Solution] The composition of the present invention comprises at least one compound selected from the group consisting of fucoxanthin and its derivatives, and linoleic acid or a salt thereof, characterized in that the weight ratio of the at least one compound selected from the group consisting of fucoxanthin and its derivatives to the linoleic acid or salt thereof ([at least one compound selected from the group consisting of fucoxanthin and its derivatives] / [linoleic acid or a salt thereof]) is 1.1 to 22.0.

The invention discloses a fucoxanthin derivative as well as a preparation method and application thereof, and belongs to the technical field of compounds. The chemical structure of the fucoxanthin derivative is as shown in formulas 1-6, and the fucoxanthin derivative with high stability is obtained by carrying out structural derivatization modification on fucoxanthin. Under the dark condition, the retention rate of the fucoxanthin derivative is higher than that of maternal fucoxanthin, the stability of the fucoxanthin derivative is better than that of fucoxanthin, under the constant illumination condition, the retention rate of the fucoxanthin derivative is high, the stability of the fucoxanthin derivative is high, and the fucoxanthin derivative can be used for preparing products or drugs for resisting oxidation, cancer and diabetes or reducing blood fat.

The invention discloses a nucleic acid aptamer OUC-Fx11-10T with high affinity to fucoxanthin, and belongs to the technical field of nucleic acid aptamers. The nucleotide sequence of the nucleic acid aptamer OUC-Fx11-10T with high affinity to fucoxanthine is as shown in SEQ ID NO. 18. The nucleic acid aptamer OUC-Fx11-10T has high affinity to fucoxanthine. The invention further discloses the application of the nucleic acid aptamer OUC-Fx11-10T in identification or detection of the fucoxanthin. According to the nucleic acid aptamer OUC-Fx11-10T with high affinity to the fucoxanthine, the affinity and dissociation constant of the nucleic acid aptamer OUC-Fx11-10T with the fucoxanthine is measured to be 86.1 nM through an isothermal titration thermal method, and the nucleic acid aptamer OUC-Fx11-10T has no obvious affinity to structural analogues of the fucoxanthine such as violaxanthin, neoxanthin, zeaxanthin and beta-carotene; the fucoxanthine aptamer has high affinity and good specificity to fucoxanthine, and can be used for identification or detection of fucoxanthine.

The invention relates to the field of natural product extraction, in particular to fucoxanthine-containing grease as well as a preparation method and application thereof, and the method comprises the following steps: mixing a seaweed raw material with diglyceride oil, and performing extraction operation, so that fucoxanthine is extracted from the seaweed raw material, and the fucoxanthine-containing grease is obtained. Compared with traditional edible oil (such as soybean oil, olive oil and the like), the diglyceride oil is adopted as an extraction medium, the extraction efficiency of fucoxanthine in seaweed is remarkably better, the content of fucoxanthine in the diglyceride oil can be optimally increased by 50% under the same condition, and therefore the yield of target active ingredients is greatly increased, and the application prospect is wide. The method is simple in process route, only relates to physical steps such as mixing, extraction and separation, does not need to use any organic solvent, fundamentally avoids safety and environmental risks caused by solvent residue and volatilization, and meets the requirements of green production.

The application discloses a spermidine composition with an anti-aging effect and a preparation method thereof, and relates to the technical field of medical cosmetics. The spermidine composition with the anti-aging effect is composed of the following components in mass parts: 5-15 parts of spermidine, 3-8 parts of fucoxanthin, 1-4 parts of a SASP inhibitor, 10-20 parts of nicotinamide, 2-6 parts of sodium hyaluronate, 12-25 parts of soybean lecithin and 3-5 parts of ergothioneine. The SASP inhibitor is a compound with a specific structure. The composition can synergistically act on multiple pathways such as cell autophagy, oxidative stress and aging-related secretory phenotype by compounding spermidine, fucoxanthin and the specific SASP inhibitor, and compared with a compounding scheme of single components or other alternative components, the composition shows a more optimal comprehensive effect in protecting skin fibroblasts and delaying cell aging.

The invention discloses algae testing equipment with a pretreatment function and a testing method, and relates to the technical field of algae testing, and the algae testing equipment comprises a testing host and a pretreatment device. Feeding, oscillation wall-breaking extraction, centrifugal separation, diversion filtration and detection output are sequentially completed in the same test host, manual transfer and multi-device switching are reduced, and batch detection efficiency and result consistency are improved. The two-way clutch mechanism is subjected to displacement control through the adjusting mechanism, so that the same pretreatment device realizes rapid and reliable switching between an oscillation mode and a centrifugal mode under the condition that a power source and a bearing piece are not replaced, and wall breaking extraction and centrifugal separation are connected in series to form a continuous and controllable process. In the oscillation mode, the end assembly drives the two-way clutch mechanism to do reciprocating motion and drives the bearing piece and a mixed solution in the double-cavity centrifugal tube to do reciprocating oscillation, so that the glass grinding beads crush algae cell walls, fucoxanthin is promoted to be released and fully mixed with the solution, and the extraction efficiency is improved.

The application discloses an alga testing device with pretreatment function and a testing method, and relates to the technical field of alga testing, which comprises a testing host and a pretreatment device. The application sequentially completes feeding, oscillation wall breaking extraction, centrifugal separation, flow guiding filtration and detection output in the same testing host, reduces manual transfer and multi-device switching, and improves batch detection efficiency and result consistency. Displacement control is performed on the two-way clutch mechanism through the blending mechanism, so that the same pretreatment device realizes quick and reliable switching between the oscillation mode and the centrifugal mode without changing the power source and the bearing, thereby connecting the wall breaking extraction and the centrifugal separation into a continuous controllable process. In the oscillation mode, the end assembly drives the two-way clutch mechanism to reciprocate, drives the bearing and the mixed solution in the double-cavity centrifugal tube to reciprocate and oscillate, breaks the alga cell wall through the glass grinding beads, promotes the release of fucoxanthin and fully mixes the solution, and improves the extraction efficiency.

The invention belongs to the technical field of marine natural product extraction and biological medicine, and particularly relates to a preparation method of fucoxanthin and application of the fucoxanthin to preparation of a product with the effect of relieving xerophthalmia. The fucoxanthin disclosed by the invention is prepared by the following steps: crushing cleaned and dried seaweed, performing alcohol extraction, extracting and purifying to obtain a fucoxanthin product. The method has the beneficial effects that the purity of the fucoxanthin prepared by adopting the method disclosed by the invention is higher, in-vivo and in-vitro experiments prove that the extracted fucoxanthin has the effect of relieving the tear secretion deficiency type xerophthalmia, and a new raw material source and theoretical support are provided for treating the xerophthalmia.

This invention provides a method for simultaneously increasing the biomass and fucoxanthin yield of *Phaeodactylum tricornutum*, utilizing a *Haloxylon ammodendron*-*Phaeodactylum tricornutum* symbiotic system to simultaneously enhance algal growth efficiency and the accumulation of the high-value product fucoxanthin. Specifically, *Haloxylon ammodendron* and *Phaeodactylum tricornutum* are co-inoculated in F / 2 medium at a bacterial-algal ratio of 10:1 to 100:1 and co-cultured under constant temperature and light for 16 days. This invention utilizes *Haloxylon ammodendron* and *Phaeodactylum tricornutum* to construct a stable and efficient algal-bacterial symbiotic system, which can simultaneously increase the biomass of *Phaeodactylum tricornutum* and the fucoxanthin content, providing a new method for the large-scale, low-cost production of fucoxanthin.

This application relates to the field of materials technology, and discloses a method for preparing and applying water-soluble fucoxanthin with lipid-lowering and weight-loss effects. The eutectic consists of fucoxanthin and a eutectic forming agent, with a molar ratio of fucoxanthin to the eutectic forming agent of 1:1 to 1:3. The eutectic forming agent is selected from one or a combination of two of L-proline, glycine, β-cyclodextrin, hydroxypropyl-β-cyclodextrin, citric acid, and vitamin C. The fucoxanthin eutectic of this application can be directly added to various foods / health foods such as beverages, baked goods, dairy products, meal replacement foods, capsules, and powders without additional modification treatment. It has good dispersibility, does not affect the sensory quality of the product, and has broad application prospects.

The invention discloses an anti-aging compound nutrient containing astaxanthin, ergothioneine and fucoxanthin, which is prepared from the following ingredients in parts by mass: 15 to 25 parts of astaxanthin powder, 10 to 20 parts of ergothioneine, 5 to 15 parts of fucoxanthin, 5 to 15 parts of fenugreek seeds, 3 to 8 parts of turmeric extracts, 3 to 8 parts of sialic acid, 1 to 5 parts of ginseng root extracts and 1 to 3 parts of rhodiola rosea extracts. According to the compound nutrient, eight active components are accurately proportioned to form the compound nutrient with a multi-channel synergistic effect, so that the multi-dimensional anti-aging effects of resisting oxidation, resisting inflammation, protecting mitochondria, maintaining telomere and the like are realized, and the problems of insufficient anti-aging effect and low bioavailability of a single component in the prior art are effectively solved.

This invention discloses a high-yield fucoxanthin and / or EPA mutant strain of *Phaeodactylum tricornutum*, its breeding method, and its applications. This invention employs ambient pressure room temperature plasma (ARTP) mutagenesis technology, combined with high-throughput flow cytometry Raman spectroscopy, to screen and obtain two mutant strains, which are named *Phaeodactylum tricornutum* (…). Phaeodactylum tricornutum X7 and X20 were deposited on January 13, 2026, at the Guangdong Provincial Microbial Culture Collection Center, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with accession numbers GDMCC No:67643 and GDMCC No:67644. These mutant strains exhibit outstanding fucoxanthin and eicosapentaenoic acid (EPA) synthesis capabilities, providing core germplasm resources for the large-scale, low-cost production of fucoxanthin and EPA from microalgae.