Method for extracting and purifying fucoxanthin

A fucoxanthin and extraction method technology, which is applied in the field of extraction and purification of fucoxanthin, can solve the problems of product safety impact, large amount of organic solvent consumption, time-consuming, etc., achieve product safety guarantee, shorten extraction time, reduce Environmental Risk Effects

Inactive Publication Date: 2015-02-04
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dimethyl sulfoxide (DMSO) has also been tried for the extraction of fucoxanthin, but DMSO has a high boiling point and is difficult to recover directly by distillation. It must be extracted with ethyl acetate under the action of ammonium sulfate, thus increasing the separation The complexity of the process and the amount of solvent used are not conducive to environmental protection. At the same time, because DMSO has certain toxicity, it also has a certain impact on the safety of the product.
In these technologies, there are disadvantages such as large amount of organic solvent and time-consuming

Method used

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  • Method for extracting and purifying fucoxanthin
  • Method for extracting and purifying fucoxanthin
  • Method for extracting and purifying fucoxanthin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Different solvents and extraction methods compare the extraction efficiency of fucoxanthin in Wakame

[0068] (1) Accurately weigh 1.0g / part of crushed undaria pinnatae dry powder in a 50mL Erlenmeyer flask, divide it into three groups, add 25mL methanol, ethanol and acetone:methanol (volume ratio 7:3), and soak in the dark at room temperature 30min; three repetitions were set for each group.

[0069] (2) Three extraction methods are used for extraction, and the extraction methods and procedures are shown in Table 1 below.

[0070] Table 1 Methods and conditions used in different extraction methods

[0071]

[0072]

[0073] (3) After the extraction, centrifuge at 5000rpm for 5min, and take the supernatant.

[0074] (4) Repeat steps (2) and (3) twice after the precipitation is added to the extraction solvent; respectively merge the supernatants extracted three times in each group, distill under reduced pressure, concentrate to dryness, and dissolve wit...

Embodiment 2

[0083] The extraction of fucoxanthin in embodiment 2 laver

[0084] (1) Weigh 1.0 g of commercially available dried seaweed cake, crush it, add 25 mL of ethanol, and soak for 30 min at room temperature in the dark.

[0085] (2) Put the above materials in a microwave oven, adjust the microwave power to 600W, control each microwave time to 5s, with an interval of 10s, and extract for 90s.

[0086] (3) Centrifuge the extract in (2) at 5000rpm for 5min, take the supernatant, concentrate it to dryness under reduced pressure at 35°C, and dissolve it with 5mL ethanol.

[0087] (4) Measure the absorbance value of the extract at 450 nm with a spectrophotometer, and calculate the content of fucoxanthin according to formula ① to be 0.654 mg.

Embodiment 3

[0088] Extraction and extraction separation of fucoxanthin in embodiment 3 Undaria pinnatae

[0089] (1) Accurately weigh 15 g of dry and pulverized Undaria pinnatifida, add 200 mL of ethanol, soak in dark at room temperature for 30 min, use the microwave extraction method in Example 1 to extract, centrifuge at 5000 rpm for 5 min, take the supernatant, and concentrate under reduced pressure to dryness Afterwards, dissolve with 10 mL ethanol.

[0090] (2) Take 5 mL of the above extract, dilute it with 25 mL of 90% methanol, add 30 mL of n-hexane for extraction, and repeat 3 times. Then add double-distilled water to the methanol / water phase until the concentration of methanol is 70%, and then extract with 25 mL of n-hexane to further separate the pigment. In order to investigate the separation of pigments and the removal of impurities by the number of extractions, each extraction and raffinate were scanned for visible spectrum. The finally obtained n-hexane phase and methanol / ...

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Abstract

The invention discloses a method for extracting and purifying fucoxanthin. According to the method, a microwave assisted extraction technology is utilized, and ethanol serves as a solvent, so that fucoxanthin in brown seaweed raw materials can be rapidly extracted; and a liquid-liquid extraction and column chromatography two-step separation process is adopted, so that the fucoxanthin can be effectively separated from chlorophyll and other impurity components. The fucoxanthin is low in toxicity, safe and environment-friendly, ethanol is taken as an extraction solvent, and the use amount of a toxic organic solvent is effectively reduced; and moreover, a microwave extraction method is adopted, extraction can be finished within 5 minutes only, and the extraction time is greatly shortened. The method disclosed by the invention is simple, rapid and high-efficiency in the whole extraction and purification process, the operation is convenient, large-scale production is easily realized, and a foundation is laid for development and utilization of natural health food additives such as the fucoxanthin with extremely high values and biological drug resources.

Description

technical field [0001] The invention relates to the fields of biotechnology, bioseparation engineering, analytical chemistry and biopharmaceutical development, and more specifically relates to a method for extracting and purifying fucoxanthin. Background technique [0002] Fucoxanthin is an important class of light-harvesting pigments found in edible brown algae such as Undaria pinnatifid. It exists widely in the thylakoid membrane of brown algae. Like β-carotene, it is one of the important natural carotenoids First, it has a variety of biological activities and has a wide range of uses in poultry aquaculture. Studies in recent years have also shown that fucoxanthin has strong anti-tumor activity, and has strong anti-tumor activity on a variety of tumor cells, such as neuroma cells, rectal cancer cells, colon cancer cells, liver cancer cells, leukemia cells, prostate cancer cells and other tumor cell lines. proliferation-inhibiting and killing effects. In addition, fucoxan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32
CPCC07D301/32
Inventor 刘永梅陈伟陈晶高永智张秀梅
Owner WUHAN UNIV
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