42 results about "Thylakoid" patented technology

The present invention relates to a process on a large industrial scale for the production of thylakoids, from photosynthetic organisms, such as from green plant leaf material, to be used as ingredients in food, or additions to food, or dietary supplements, or pharmaceuticals for the purpose of preventing overweight, promoting satiety, reducing food intake, reducing bodyweight, reducing blood insulin concentration and reducing blood fats and percentage body fat in humans and animals.

TGD2 proteins of Arabidopsis are proposed to be a substrate binding component of a lipid transfer complex in the inner chloroplast envelope membrane. Loss of function of this protein or other components of this complex may disrupt the endoplasmic reticulum (ER)-pathway of thylakoid lipid biosynthesis. In one embodiment, the present invention contemplates a minimal binding domain capable of specifically binding phosphatidic acid. Alternatively, the minimal binding domain may further comprise accessory binding domains that, in combination, create a complete TGD2 phosphatidic acid binding domain. Consequently, phosphatidic acid may be quantitatively detected from samples as described in the methods herein.

The invention discloses a blue green algae growth inhibitor which comprises the following components by weight: 10%-25% of plant extract, 1.5%-5% of a blue green algae complexing agent, 1.5%-3% of blue green algae growth inhibin, 20%-35% of a synergist, 1%-10% of rare earth, 30%-40% of diatom forming soil, 2.5%-8.7% of a bio-enzyme microbial inoculums and 0.5%-10% of humic acid powder. The blue green algae growth inhibitor can be used for killing blue green algae in a long time, and can deprive compulsively blue green algae cell thylakoid pseudo telescopic function for solidifying, sinking, rigidifying and dying of blue green algae cells, eliminating of water bloom and purifying of water quality. The blue green algae growth inhibitor can effectively kill spirogyra, hydrodictyon, chaetophora and bottleneck algae and other mud moss filamentous capsules in an aquaculture pond, and can eliminate a large number of algae attached to meshes in cage culture, and after biological algae removal, beneficial algae and beneficial microorganisms are recultivated to add nutrition balance elements for reactivating the water quality.

The invention discloses a rice ageing-relevant control gene OsCDC48E sequence and a coded translated protein and an amino acid sequence thereof. Through a map-based cloning technology, a gene OsCDC48E for controlling rice leaf ageing is obtained from a rice premature senile mutant W95 through cloning, and functional complementation experiments prove that the OsCDC48E is a gene which controls the rice growth and development and is relevant to leaf ageing. Observation of a transmission electron microscope, and analysis of a photosynthetic rate and a photoreaction and dark reaction detection technology prove that a premature senile gene cloned from the OsCDC48E has a remarkable adjusting function of growth and development of rice, integrity of thylakoids in chloroplast, structures of organelle of endoplasmic reticulum, and the like. By using a genetic engineering technology, multiple specific marker characters of the ageing gene can be applied to rice variety improvement, yield increase and cultivation of super rice.

The invention relates to a preparation method of thylakoid-containing artificial cells and a photosynthesis simulating method, and belongs to the fields of biotechnology, biochemistry and synthetic biology. The method comprises the following steps of extracting thylakoid particles from fresh spinach leaves, and storing the thylakoid particles in a buffer solution for use; smearing a phospholipid solution onto an ITO glass electrode; after the solvent volatilization, forming a layer of phospholipid membrane; fixing a polytetrafluoroethylene framework by vacuum grease; adding liquid phases intothe polytetrafluoroethylene framework; applying alternating current onto the ITO glass electrode for preparing a GUV solution; mixing the GUV solution and a thylakoid solution; preparing the thylakoid-containing artificial cells. The thylakoid-containing artificial cells are prepared through the enlightenment of the natural plant cell structure and function; the model can realize the optical driveelectron transfer process under the artificial sunlight irradiation. Through the model building, the attributes of chloroplasts in nature are doubly simulated from the structure and the function. Thecomplicated cells realize simplified research, so that the development and the progress of the biomimetic chemistry are promoted.

The invention relates to an extracting and purifying method of a chlorella lutein protein complex. The extracting and purifying method comprises the following steps: carrying out wall-breaking treatment onto chlorella by utilizing an ultrasonic crushing method; carrying out solubilization onto chromoprotein on a thylakoid membrane by adopting a surfactant; and purifying the lutein protein complex by adopting ammonium sulfate classification and sedimentation, ion exchange chromatography and gel filtration chromatography. A pigment composition of the complex is analyzed by utilizing full-wavelength scanning and high performance liquid chromatography, wherein the obtained lutein protein complex has remarkable ABTS radical-scavenging activity. The extracting and purifying method disclosed by the invention can ensure structure and function stability of the complex by utilizing surfactant solubilizing chromoprotein; purified lutein protein complex obtained by column chromatography is simple in purifying process and convenient to produce and expand; and the lutein existing in the form of the complex not only improves stability, but also keeps antioxidant activity.

The present invention relates to a process on a large industrial scale for the production of thylakoids, from photosynthetic organisms, such as from green plant leaf material, to be used as ingredients in food, or additions to food, or dietary supplements, or pharmaceuticals for the purpose of preventing overweight, promoting satiety, reducing food intake, reducing bodyweight, reducing blood insulin concentration and reducing blood fats and percentage body fat in humans and animals.

The invention discloses application of a chlorosis seedling lethality 1 (CSL1) gene to regulation of development of rice chloroplasts. According to the invention, a lethal rice mutant at an etiolatedseedling stage is isolated from a rice T-DNA mutant library; the lethal rice mutant is inserted into a seventh intron of the CSL1 gene by means of T-DNA insertion; a 109bp sequence is deleted at a T-DNA insertion position; the chlorophyll content of the CSL1 mutant decreases; single-layer or multi-layer autophagosomes appear in the mesophyll cells; starch grains in the chloroplasts increase; a thylakoid structure is disordered; and the expression quantity of chloroplast-related genes is changed. meanwhile, the invention further constructs a knockout plant (the CSL1 gene has a deletion of one base A at the site 46 of exon 5, or a deletion of one base A at the site 47 and substitution of the base A with the base T at the site 48) by means of gene knockout, and the knockout plant has the samephenotype as the CSL1 mutant, thus showing that the CSL1 gene is involved in regulation of development of the rice chloroplasts.

The invention provides a fusion vesicle derived from bacteria and plants as well as a preparation method and application of the fusion vesicle, and relates to the technical field of biomedicine. The fusion vesicle comprises a mixed membrane structure formed by fusing a bacterial outer membrane vesicle and a plant thylakoid membrane, wherein the bacteria are gram negative bacteria, and the plant thylakoid is a spinach thylakoid. The fusion vesicle of the present invention combines the characteristics of the bacterial outer membrane vesicle and the plant capsular membrane, the structure of the vesicle is uniform, the vesicle can efficiently and rapidly reach the tumor, the tumor targeting property is high, and the accumulation time is long; and the obvious effect is realized in the aspect of tumor treatment.

The invention relates to a composite material for simultaneously generating oxygen and active oxygen under near-infrared light excitation, and a preparation method and application of the composite material, and belongs to the technical field of composite materials. The technical problem that an oxygen generation manner during the tumor photodynamic therapy in the prior art cannot simultaneously realize the precise matching of oxygen and a photosensitizer both in space and time, and the PDT efficiency is greatly affected due to rapid oxygen consumption and slow spatial transmission is solved. The composite material of the invention is composed of up-conversion nanoparticles and thylakoid membranes. The invention also provides a preparation method and application of the composite material. The up-conversion nanomaterial in the composite material of the invention can convert near-infrared light (808-980 nm) into red light (-660 nm), the red light is used to excite a light system I and a light system II in the thylakoid membranes, photo-generated holes generated by the light system I are used to react with water to generate oxygen, the light systems I and II transfer energy to oxygen molecules to generate singlet oxygen, and thereby the tumor photodynamic therapy efficiency is improved.

The invention discloses nicotiana benthamiana TMP14 (Thylakoid Membrane Phosphoprotein 14) and application thereof in plant virus resistance. The amino acid sequence of the nicotiana benthamiana TMP14is as shown in the sequence table SEQ ID NO.1. The invention also discloses an optimized mutant of the nicotiana benthamiana TMP14. The nicotiana benthamiana TMP14 disclosed by the invention directlyinteracts with TSWV (Tomato Spotted Wilt Virus) NSm; the over-expression transgenic tobacco plant of the nicotiana benthamiana TMP14 has the function of resisting plant virus; the discovery providestheoretical and practical basis for researching transgenic anti-virus crops, and has a broad application prospect.

The invention discloses a photocatalytic material capable of rapidly capturing carbon dioxide and releasing oxygen. According to the invention, a thylakoid / manganese-based light-capturing oxygen-releasing composite material is prepared by utilizing photocatalytic oxygen-releasing properties of extracted in-vitro thylakoid and manganese dioxide through a material compounding preparation method, sothat the photosynthesis efficiency of the thylakoid is further improved; and the material has advantages of easy obtaining, simple preparation method, mild preparation condition, environmental protection, no pollution, biodegradability and the like, and has potential application prospects in the field of environmental protection, especially air purification.

The invention belongs to the technical field of biological separation engineering, and particularly relates to a method for extracting cyst-like protein. At present, a method for extracting the cyst-like protein does not exist. The invention provides the method for extracting the cyst-like protein, which comprises the following steps: 1) cutting leaves into pieces, putting the pieces into a grinding buffer solution GB, and crushing; 2) separating chloroplast in the broken leaf cells, and obtaining the chloroplast with high purity and complete form; 3) labeling a sample to be subjected to component separation, and then centrifuging; 4) resuspending a sample for component separation by using a sucrose-containing 1*TE buffer, and cracking chloroplast; and 5) separating a supernatant from theprecipitate through centrifugation to obtain the cyst-like protein, which is mainly used for analyzing and researching important functions and action mechanisms of the cyst-like protein in various life activities, and has important biological significance for explaining physiological functions and genetic rules of the cyst-like protein.

The invention provides hypocrellin composite nano particles and a preparation method thereof.The nano particles are composed of hypocrellin nano particles and nano capsule-like membranes coating the surfaces of the hypocrellin nano particles, and the preparation method comprises the steps that water-insoluble hypocrellin is dissolved in a dimethyl sulfoxide solution, then the solution is dropwise added into a phosphate buffer solution, and the hypocrellin nano particles are obtained. Performing ultrasonic dispersion treatment and dialysis, or dispersing the hypocrellin in a poloxamer aqueous solution, and performing ultrasonic dispersion to obtain a hypocrellin nanoparticle suspension; then modifying the surface of the hypocrellin nanoparticle suspension with a suspension of a thylakoid membrane extracted from green plant cells, or modifying the surface of the hypocrellin nanoparticle suspension with a suspension of a thylakoid membrane extracted from microalgae cells, and extruding the hypocrellin nanoparticle suspension through a microfiltration membrane; the composite nanoparticles have the capability of producing oxygen through photosynthesis under illumination, and can provide oxygen for the photodynamic effect or the sonodynamic effect of the hypocrellin, so that the level of singlet oxygen produced by the hypocrellin under the action of illumination or ultrasonic waves is improved.

The invention discloses a thylakoid lipid solid supporting membrane and a construction method thereof. The construction method comprises the following steps: providing a substrate, and modifying the surface of the substrate by hydrophilic polymer molecules to form a hydrophilic polymer molecule modified substrate; providing a thylakoid lipid vesicle, and integrating a photosystem II (PSII) into the thylakoid lipid vesicle to form a thylakoid lipid vesicle integrated with the PSII; and when the content of cholesterol in the thylakoid lipid vesicle is 3-8% mol and the concentration of Ca<2+> ina solution is 15-25 mM, hatching the thylakoid lipid vesicle integrated with the PSII on the surface of the hydrophilic polymer molecule modified substrate to obtain the thylakoid lipid solid supporting membrane integrated with the PSII. Through the method for constructing the thylakoid lipid vesicle solid supporting membrane provided by the invention, in-vitro activity of the PSII can be obviously improved.

The invention discloses application of an FtsHi5 gene of arabidopsis thaliana in regulation and control of development of chloroplast of arabidopsis thaliana, and relates to the technical field of genetic engineering. According to the application, an interference vector of the FtsHi5 gene of the arabidopsis thaliana is constructed, and wild-type arabidopsis thaliana is transfected; the obtained FtsHi5 interference mutant shows yellowing and dwarfness when DEX is added, the chlorophyll content is reduced, and a thylakoid structure in the chloroplast is damaged. Experiments prove that the FtsHi5gene of the arabidopsis thaliana participates in the regulation and control of the development of the chloroplast, thus being capable of regulating and controlling the development of the chloroplast.

TGD2 proteins of Arabidopsis are proposed to be a substrate binding component of a lipid transfer complex in the inner chloroplast envelope membrane. Loss of function of this protein or other components of this complex may disrupt the endoplasmic reticulum (ER)-pathway of thylakoid lipid biosynthesis. In one embodiment, the present invention contemplates a minimal binding domain capable of specifically binding phosphatidic acid. Alternatively, the minimal binding domain may further comprise accessory binding domains that, in combination, create a complete TGD2 phosphatidic acid binding domain. Consequently, phosphatidic acid may be quantitatively detected from samples as described in the methods herein.

The invention relates to a method for preparing a nanometer light adsorption material based on a plant thylakoid structure, belonging to the technical field of nanometer semiconductors. The nanometer light adsorption material is prepared by extracting chloroplast from fresh plant blades, mixing the chloroplast with precursor titanium tetrachloride solution and baking. The implemented object in the invention has low cost, rich resources, simple process, convenient operation and low cost in the synthetic process. The constructed nanometer material represents the coupling function of the structural specificity and optical property of replica and improves the light adsorption performance of the material.

The invention relates to a composite material for simultaneously generating oxygen and active oxygen under near-infrared light excitation, a preparation method and application thereof, and belongs to the technical field of composite materials. Solve the technical problems in the prior art that the oxygen production method in the photodynamic therapy of tumors cannot realize the precise matching of oxygen and photosensitizer in space and time at the same time, and the rapid oxygen consumption and slow space transfer greatly affect the PDT efficiency. The composite material of the invention is composed of up-converting nanoparticles and thylakoid membranes. The invention also provides the preparation method and application of the composite material. The composite material of the present invention can convert near-infrared light (808-980nm) into red light (~660nm) in the mid-up conversion nanomaterial, which is used to excite photosystem I and photosystem II in thylakoid membranes, and photosystem I produces Photogenerated holes are used to react with water to generate oxygen, and photosystems I and II transfer energy to oxygen molecules and generate singlet oxygen, thereby improving the efficiency of tumor photodynamic therapy.

The invention discloses application of a nanometer capsule body to preparation of anti-tumor medicines. Through the study, the inventor discovers that the nanometer capsule body can promote hydrogen peroxide accumulated in the tumor region to be decomposed into oxygen so that the tumor anoxia can be overcome; meanwhile, the light sensitive characteristic is realized, so that the nanometer capsulebody can be used as a light sensitive agent; the light power anti-tumor operation can be performed, so that the effect of combining the dual functions is achieved. Compared with a single PDT nanometermaterial, the nanometer capsule body has obviously improved PDT treatment effect.

The invention discloses nicotiana benthamiana TMP14 (Thylakoid Membrane Phosphoprotein 14) and application thereof in plant virus resistance. The amino acid sequence of the nicotiana benthamiana TMP14is as shown in the sequence table SEQ ID NO.1. The invention also discloses an optimized mutant of the nicotiana benthamiana TMP14. The nicotiana benthamiana TMP14 disclosed by the invention directlyinteracts with TSWV (Tomato Spotted Wilt Virus) NSm; the over-expression transgenic tobacco plant of the nicotiana benthamiana TMP14 has the function of resisting plant virus; the discovery providestheoretical and practical basis for researching transgenic anti-virus crops, and has a broad application prospect.

The invention discloses a rice lesion aging regulation gene and application thereof. The rice lesion aging regulation gene is named as rice lesion aging regulation gene LEMS3, and the nucleotide sequence of the rice lesion and senescence regulation gene LEMS3 is as shown in SEQ ID NO.1. The gene LMES3 for controlling rice lesion senescence is cloned from a rice lesion premature senescence mutant lmes3 through a map-based cloning technology, and a functional complementation experiment proves that the LMES3 is a related gene for controlling rice senescence. Through observation of a transmission electron microscope, a photosynthetic rate, a photoreaction detection technology and a dark reaction detection technology, the premature senility gene cloned by the invention is proved to have an obvious regulation effect on growth and development of rice, completeness of thylakoid in chloroplast and the like. The invention also provides application of the gene. Some specific marker characters of the senescence gene can be applied to rice variety improvement, yield increase and resistance screening by utilizing a gene engineering technology.

The invention discloses a method for rapidly purifying cytochrome b6f, wherein the method comprises the steps: by taking cyanobacteria as a raw material, breaking cells to obtain a thylakoid membrane, washing to remove impure protein, adding a detergent undecyl maltoside or dodecyl-beta-D-maltoside, and obtaining a crude product of cytochrome b6f by adopting a three-step ammonium sulfate precipitation method; and performing sucrose density gradient centrifugation and anion exchange column treatment to obtain the cytochrome b6f finished product. Compared with the prior art, the method has the advantages that the cytochrome b6f is purified by taking cyanobacteria which is high in growth speed and simple to culture as a raw material, the extraction rate is high, the operation steps are few, the consumed time is short, and the obtained cytochrome b6f is high in purity and good in uniformity.

The invention discloses a method for inhibiting harmful algae by applying perylenequinones compounds, and belongs to the technical field of environmental application. The perylenequinone compound provided by the invention can obviously inhibit the growth of harmful algae in the absence of illumination or visible illumination radiation, so that the cell surface is unsmooth and the cell morphology is incomplete; cell membranes, cell walls and thylakoid structures can be destroyed; degrading fatty acid in a cyanobacteria cell membrane; the content of chlorophyll a is degraded; growth of blue-green algae in the actual environment of the Taihu Lake is According to the method, the perylenequinone compound is used as the algistat for broad-spectrum inhibition of blue-green algae, and the application prospect is very wide.

The invention discloses a thylakoid lipid solid support membrane and a construction method thereof. The construction method includes: providing a substrate, and modifying the surface of the substrate with hydrophilic polymer molecules to form a substrate modified by hydrophilic polymer molecules; providing thylakoid lipid vesicles, and integrating PSII into the thylakoid In lipid vesicles, thylakoid lipid vesicles integrated with PSII are formed; the content of cholesterol in thylakoid lipid vesicles is 3-8% mol, Ca in solution 2+ When the concentration is 15-25 mM, the thylakoid lipid vesicle integrated with PSII is incubated on the substrate surface modified by the hydrophilic polymer molecules to obtain a solid support membrane of thylakoid lipid integrated with PSII. With the method for constructing thylakoid lipid solid support membrane provided by the present invention, the in vitro activity of PSII can be significantly improved.

The invention discloses an artificial thylakoid microfluidic chip and a preparation method thereof. There are photocatalytic materials, the inner surface of the lower structure of the artificial thylakoid microfluidic chip channel is fixed with oxidoreductase, and the fixed materials on the surface of the upper and lower structures do not contact and interfere with each other, and are applied in high-efficiency photocatalytic nicotinyl coenzyme regeneration aspect. The existing photocatalytic coenzyme regeneration system solves the shortcomings of complex photocatalytic material recovery steps, low utilization rate of oxidoreductase, poor stability, difficult recovery, and poor compatibility between photocatalytic materials and biocatalytic enzymes.

A method of forming a stable protein complex comprising: providing aqueous protein complexes, wherein the protein complexes are one or more of photosystem I complex from spinach, photosystem II complex from spinach, chlorophyll antennae, thylakoids, bacteriochlorophylls, chlorosomes, and photosystems from green algae, cyanobacteria, and plants; cationizing the aqueous protein complexes by the addition of stoichiometric amounts of a crosslinker in the presence of a coupling reagent; titrating the cationized protein complexes with a counter anionic polymer until the protein cation / anion pair solution becomes negative by zeta potential measurement, to create at least one antibody cation / anion pair in aqueous solution. The protein complexes cation / anion pair solution may be lyophilized to remove all of the water, forming a lyophilized solid. The lyophilized solid may be heated until a protein complex ionic liquid is generated. The cationized protein complexes may be purified from excess coupling reagents by dialysis in water.

The invention relates to related protein capable of adjusting translation efficiency of chloroplast protein and improving heat resistance of plants, and application thereof. In particular, the inventor screens one mutant rps1 related to the translation efficiency of the chloroplast protein and the heat resistance of the plants from various different arabidopsis mutants, and identifies a plant chloroplast ribosome small subunit S1 (RPS1) gene from the mutant; and the gene and polypeptide can effectively maintain the heat stability of a thylakoid membrane, can improve the translation efficiency of the chloroplast protein of the plants, can improve the survival ability and the photosynthesis efficiency of the plants under heat stress, and can effectively avoid and reduce adverse influence of high temperature on the plants.

The invention discloses an application of a gene CDA1 in regulation and control of chloroplast development, and belongs to the technical field of plant genetic engineering and cell biology. Accordingto the invention, an interference vector of an Arabidopsis thaliana CDA1 gene is constructed and then wild Arabidopsis thaliana is transfected to obtain an interfered strain. A research finds that cotyledons of the interfered strain show different degrees of green loss, and chloroplasts have no thylakoid membrane structure and are severe in vacuolation. According to the invention, the mutant cda1is obtained by inserting T-DNA into the Arabidopsis thaliana CDA1 gene, and the accumulation amount of photosynthetic proteins of the mutant cda1 is greatly reduced compared with the accumulation amount of photosynthetic proteins of the wild Arabidopsis thaliana. Experiments prove that the CDA1 gene participates in regulation and control of chloroplast development, influences accumulation of plantphotosynthetic protein, can be used for researching development and photosynthesis of plant chloroplasts, and provides theoretical guidance for research of development of chloroplasts of the plant and regulation and control of photosynthetic performance of the plant.