9 results about "Thylakoid" patented technology

The invention discloses a method for researching influence on hemolytic activity of marine microalgae based on a transcriptome technology, and relates to the field of hemolytic activity analys.The method comprises the steps that pretreatment is conducted on the marine microalgae based on culture requirements, and cystic morphological cell density and hemolytic activity of the marine microalgae are measured according to experimental design rules after pretreatment is completed; carrying out transcriptome sample collection operation by utilizing the marine microalgae subjected to pretreatment, constructing a library according to a transcriptome sample collection result, and obtaining a gene function annotation and a differential expression gene result; the growth conditions of the marine microalgae under different temperature conditions are analyzed according to cystic morphological cell density and hemolytic activity, and the regulatory gene influencing the hemolytic activity of the marine microalgae is obtained by combining gene function annotation and differential expression gene results. According to the method, the hemolytic activity of the marine microalgae cultured under different temperature conditions is extracted, so that the aim of researching related metabolic pathways possibly participating in toxin synthesis and hemolytic activity regulation is fulfilled.

The present invention provides a use of a functional thylakoid extract, particularly in combination with a physiologically acceptable carrier, for medical purposes in wound healing. The thylakoid extract can be used to treat wounds in the skin or mucosa, such as open wounds or wounds caused by physical trauma. The thylakoid extract can be used to increase the production or level of GM-CSF growth factor and / or G-CSF growth factor in the skin or mucosa of a subject after wounding (e.g., wounds in the skin, mucosa, or corneal epithelium).

The invention relates to the technical field of photosynthesis power generation, and discloses a device and a method for realizing photosynthesis power generation based on a liquid metal-semiconductor junction. The negative electrode comprises a metal-semiconductor junction (MSJs) consisting of liquid metal and a silicon wafer; the thylakoid suspension is positioned between the positive electrode and the negative electrode and is extracted from spinach. The liquid metal is eGaInSn room temperature liquid metal. The method comprises the following steps: extracting crushed chloroplasts and thylakoid which are rich in photosynthetic devices such as a photosystem I (PSI), a photosystem II (PS) and the like in spinach leaves by using a salt solution separation method; in combination with flexible transparent indium tin oxide (ITO), the photosynthesis power generation device with the structure of Si / LMs / BBY / ITO is manufactured. The liquid metal and the silicon wafer are combined to obtain the MSJs as the negative electrode, the electron transfer efficiency of the power generation device is enhanced, the preparation is simple, the performance is excellent, and green power generation is realized.

A method for extracting chromoplasts is provided. Plant tissues are added with an extraction solvent, homogenized and filtered to collect a filtrate, which is further centrifuged. The supernatant is discarded, and the precipitate is collected as a crude extract. The crude extract is subjected to gradient separation with a series of sucrose solutions. A chromoplast-enriched layer is pipetted, added with the extraction solvent and centrifuged. The precipitate is collected as a purified chromoplast extract. A chromoplast extract obtained by the method and an application thereof are also provided.

This invention discloses a photosynthetic nanomotor powered by a biomolecular motor and its preparation method, belonging to the field of colloidal motors. This invention aims to address the current limitations of research on the rotational dynamics of FoF1-ATP synthase molecular machines, which is limited to the motion of single and isolated objects. The photosynthetic nanomotor is prepared based on supramolecular self-assembly technology through the reconstruction of thylakoid vesicles and lecithin vesicles. The photophosphorylation system on the thylakoid membrane surface is reconstructed on the photosynthetic nanomotor. Utilizing the preserved biomolecular motor FoF1-ATPase as the power engine, under external visible light irradiation, protons generated by the photosystem accumulate in the photosynthetic liposome motor, driving the FoF1-ATPase to synthesize the energy currency ATP. Through metabolic reactions, light energy is converted into chemical energy ATP, ultimately generating self-propulsion. Due to its unique energy currency regeneration capability, the photosynthetic liposome motor has broad application prospects in the biomedical field.

The invention belongs to the field of synthetic biology, and discloses an artificial carbon dioxide fixation method and application thereof. According to the method, an artificial carbon dioxide immobilization metabolic pathway is constructed, the pathway is a linear autocatalytic cycle pathway LATCH, acetyl coenzyme A and derivative products thereof can be synthesized by using carbon dioxide or bicarbonate, the catalytic cycle pathway takes two molecules of CO2 or HCO3 <-> as a substrate, one molecule of acetyl coenzyme A is generated through ten enzymatic steps, operation of the pathway depends on ATP and NADPH, and the acetyl coenzyme A can be synthesized by using the linear autocatalytic cycle pathway LATCH. Or is driven by an energy and reducing power supply system, such as organic matters such as glucose, formic acid and the like, high-energy compounds such as polyphosphoric acid, phosphocreatine and the like, or a photoreaction enzyme complex on a plant capsule membrane. An in-vitro multi-enzyme system and recombinant escherichia coli experiment prove that the acetyl coenzyme A and the derivative polyhydroxybutyric acid thereof can be efficiently synthesized, and the application prospect in the fields of carbon neutralization, green manufacturing and the like is wide.

The present application relates to a monocyte-carrying type oxygen-producing liposome and a preparation method and application thereof, wherein the preparation method comprises: synthesizing a lipopeptide CP with a cysteine terminal and a lipid RL containing a sulfur ketal domain through a solid-phase polypeptide synthesis method, extracting a thylakoid membrane TK, dissolving and uniformly mixing the lipid components and the lipopeptide CP using an organic solvent, dissolving and uniformly mixing the lipid RL and a STING agonist DA using an organic solvent, and removing the organic solvent in the mixed solution; adding an aqueous solution to the lipid membrane to hydrate, forming a crude liposome suspension, fusing the thylakoid membrane TK to the liposome using an ultrasonic-extrusion method, and obtaining a liposome product. Compared with the prior art, the liposome prepared by the present application can efficiently deliver DA to the deep part of pancreatic cancer, activate multiple cell STING pathways and immune responses, relieve hypoxia, improve immune suppression caused by hypoxia, and thus enhance the immunotherapy effect of DA.

A method for preparing biomimetic nanocomposite materials based on thylakoid membranes is disclosed, belonging to the field of bionanomaterials technology. This invention addresses the problems of existing nano-manganese oxide, which is easily cleared by the immune system, has difficulty penetrating complex tumor matrixes, and suffers from complex synthesis methods and high costs; it also addresses the issues of insufficient rigidity, instability, inability to accurately reach tumor sites, and limited functionality in existing single-membrane structures. The method comprises: 1. Dispersing a thylakoid membrane in pure water, then heating and adding hydrochloric acid solution to obtain a Mg-removed Nks system; 2. Adding an aqueous manganese chloride solution to the Mg-removed Nks system, then adjusting the pH and continuing stirring. This invention is used for the preparation of biomimetic nanocomposite materials based on thylakoid membranes.

The invention provides a method for synthesizing p-hydroxybenzaldehyde from p-hydroxybenzoic acid through light-driven enzyme catalysis. A used reaction system comprises carboxylic acid reductase (CAR), a photosynthesis unit, ferredoxin (Fdx) and phosphotransferase (PAP). According to the method, light energy is utilized to drive spinach capsule membranes to co-regenerate NADPH and ATP, and the problem that carboxylic acid reductase needs coenzyme supply of NADPH and ATP at the same time is solved. According to the method, a light energy driven enzyme catalysis method is adopted, a biological photosynthetic reaction is used for replacing a traditional high-energy-consumption and high-pollution chemical process, and green and efficient synthesis of p-hydroxybenzaldehyde is achieved through cooperation of double-coenzyme light regeneration and enzyme specific catalysis.