42 results about "Thylakoid" patented technology

The invention discloses a blue green algae growth inhibitor which comprises the following components by weight: 10%-25% of plant extract, 1.5%-5% of a blue green algae complexing agent, 1.5%-3% of blue green algae growth inhibin, 20%-35% of a synergist, 1%-10% of rare earth, 30%-40% of diatom forming soil, 2.5%-8.7% of a bio-enzyme microbial inoculums and 0.5%-10% of humic acid powder. The blue green algae growth inhibitor can be used for killing blue green algae in a long time, and can deprive compulsively blue green algae cell thylakoid pseudo telescopic function for solidifying, sinking, rigidifying and dying of blue green algae cells, eliminating of water bloom and purifying of water quality. The blue green algae growth inhibitor can effectively kill spirogyra, hydrodictyon, chaetophora and bottleneck algae and other mud moss filamentous capsules in an aquaculture pond, and can eliminate a large number of algae attached to meshes in cage culture, and after biological algae removal, beneficial algae and beneficial microorganisms are recultivated to add nutrition balance elements for reactivating the water quality.

The invention discloses a rice ageing-relevant control gene OsCDC48E sequence and a coded translated protein and an amino acid sequence thereof. Through a map-based cloning technology, a gene OsCDC48E for controlling rice leaf ageing is obtained from a rice premature senile mutant W95 through cloning, and functional complementation experiments prove that the OsCDC48E is a gene which controls the rice growth and development and is relevant to leaf ageing. Observation of a transmission electron microscope, and analysis of a photosynthetic rate and a photoreaction and dark reaction detection technology prove that a premature senile gene cloned from the OsCDC48E has a remarkable adjusting function of growth and development of rice, integrity of thylakoids in chloroplast, structures of organelle of endoplasmic reticulum, and the like. By using a genetic engineering technology, multiple specific marker characters of the ageing gene can be applied to rice variety improvement, yield increase and cultivation of super rice.

The present invention relates to a process on a large industrial scale for the production of thylakoids, from photosynthetic organisms, such as from green plant leaf material, to be used as ingredients in food, or additions to food, or dietary supplements, or pharmaceuticals for the purpose of preventing overweight, promoting satiety, reducing food intake, reducing bodyweight, reducing blood insulin concentration and reducing blood fats and percentage body fat in humans and animals.

The invention provides hypocrellin composite nano particles and a preparation method thereof.The nano particles are composed of hypocrellin nano particles and nano capsule-like membranes coating the surfaces of the hypocrellin nano particles, and the preparation method comprises the steps that water-insoluble hypocrellin is dissolved in a dimethyl sulfoxide solution, then the solution is dropwise added into a phosphate buffer solution, and the hypocrellin nano particles are obtained. Performing ultrasonic dispersion treatment and dialysis, or dispersing the hypocrellin in a poloxamer aqueous solution, and performing ultrasonic dispersion to obtain a hypocrellin nanoparticle suspension; then modifying the surface of the hypocrellin nanoparticle suspension with a suspension of a thylakoid membrane extracted from green plant cells, or modifying the surface of the hypocrellin nanoparticle suspension with a suspension of a thylakoid membrane extracted from microalgae cells, and extruding the hypocrellin nanoparticle suspension through a microfiltration membrane; the composite nanoparticles have the capability of producing oxygen through photosynthesis under illumination, and can provide oxygen for the photodynamic effect or the sonodynamic effect of the hypocrellin, so that the level of singlet oxygen produced by the hypocrellin under the action of illumination or ultrasonic waves is improved.

TGD2 proteins of Arabidopsis are proposed to be a substrate binding component of a lipid transfer complex in the inner chloroplast envelope membrane. Loss of function of this protein or other components of this complex may disrupt the endoplasmic reticulum (ER)-pathway of thylakoid lipid biosynthesis. In one embodiment, the present invention contemplates a minimal binding domain capable of specifically binding phosphatidic acid. Alternatively, the minimal binding domain may further comprise accessory binding domains that, in combination, create a complete TGD2 phosphatidic acid binding domain. Consequently, phosphatidic acid may be quantitatively detected from samples as described in the methods herein.

The invention relates to a method for preparing a nanometer light adsorption material based on a plant thylakoid structure, belonging to the technical field of nanometer semiconductors. The nanometer light adsorption material is prepared by extracting chloroplast from fresh plant blades, mixing the chloroplast with precursor titanium tetrachloride solution and baking. The implemented object in the invention has low cost, rich resources, simple process, convenient operation and low cost in the synthetic process. The constructed nanometer material represents the coupling function of the structural specificity and optical property of replica and improves the light adsorption performance of the material.

The invention relates to a composite material for simultaneously generating oxygen and active oxygen under near-infrared light excitation, a preparation method and application thereof, and belongs to the technical field of composite materials. Solve the technical problems in the prior art that the oxygen production method in the photodynamic therapy of tumors cannot realize the precise matching of oxygen and photosensitizer in space and time at the same time, and the rapid oxygen consumption and slow space transfer greatly affect the PDT efficiency. The composite material of the invention is composed of up-converting nanoparticles and thylakoid membranes. The invention also provides the preparation method and application of the composite material. The composite material of the present invention can convert near-infrared light (808-980nm) into red light (~660nm) in the mid-up conversion nanomaterial, which is used to excite photosystem I and photosystem II in thylakoid membranes, and photosystem I produces Photogenerated holes are used to react with water to generate oxygen, and photosystems I and II transfer energy to oxygen molecules and generate singlet oxygen, thereby improving the efficiency of tumor photodynamic therapy.

The invention discloses application of a nanometer capsule body to preparation of anti-tumor medicines. Through the study, the inventor discovers that the nanometer capsule body can promote hydrogen peroxide accumulated in the tumor region to be decomposed into oxygen so that the tumor anoxia can be overcome; meanwhile, the light sensitive characteristic is realized, so that the nanometer capsulebody can be used as a light sensitive agent; the light power anti-tumor operation can be performed, so that the effect of combining the dual functions is achieved. Compared with a single PDT nanometermaterial, the nanometer capsule body has obviously improved PDT treatment effect.

The invention discloses nicotiana benthamiana TMP14 (Thylakoid Membrane Phosphoprotein 14) and application thereof in plant virus resistance. The amino acid sequence of the nicotiana benthamiana TMP14is as shown in the sequence table SEQ ID NO.1. The invention also discloses an optimized mutant of the nicotiana benthamiana TMP14. The nicotiana benthamiana TMP14 disclosed by the invention directlyinteracts with TSWV (Tomato Spotted Wilt Virus) NSm; the over-expression transgenic tobacco plant of the nicotiana benthamiana TMP14 has the function of resisting plant virus; the discovery providestheoretical and practical basis for researching transgenic anti-virus crops, and has a broad application prospect.

The present invention provides a method of producing a higher plant having cytochrome c6 in the thylakoid space of chloroplast, which is characterized in that it comprises introducing a gene encoding a fused protein formed by adding a signal peptide consisting of 50 to 80 amino acid residues to a cytochrome c6 protein into the genome of a higher plant.

The invention discloses a thylakoid lipid solid support membrane and a construction method thereof. The construction method includes: providing a substrate, and modifying the surface of the substrate with hydrophilic polymer molecules to form a substrate modified by hydrophilic polymer molecules; providing thylakoid lipid vesicles, and integrating PSII into the thylakoid In lipid vesicles, thylakoid lipid vesicles integrated with PSII are formed; the content of cholesterol in thylakoid lipid vesicles is 3-8% mol, Ca in solution 2+ When the concentration is 15-25 mM, the thylakoid lipid vesicle integrated with PSII is incubated on the substrate surface modified by the hydrophilic polymer molecules to obtain a solid support membrane of thylakoid lipid integrated with PSII. With the method for constructing thylakoid lipid solid support membrane provided by the present invention, the in vitro activity of PSII can be significantly improved.

The invention discloses an artificial thylakoid microfluidic chip and a preparation method thereof. There are photocatalytic materials, the inner surface of the lower structure of the artificial thylakoid microfluidic chip channel is fixed with oxidoreductase, and the fixed materials on the surface of the upper and lower structures do not contact and interfere with each other, and are applied in high-efficiency photocatalytic nicotinyl coenzyme regeneration aspect. The existing photocatalytic coenzyme regeneration system solves the shortcomings of complex photocatalytic material recovery steps, low utilization rate of oxidoreductase, poor stability, difficult recovery, and poor compatibility between photocatalytic materials and biocatalytic enzymes.

The invention relates to related protein capable of adjusting translation efficiency of chloroplast protein and improving heat resistance of plants, and application thereof. In particular, the inventor screens one mutant rps1 related to the translation efficiency of the chloroplast protein and the heat resistance of the plants from various different arabidopsis mutants, and identifies a plant chloroplast ribosome small subunit S1 (RPS1) gene from the mutant; and the gene and polypeptide can effectively maintain the heat stability of a thylakoid membrane, can improve the translation efficiency of the chloroplast protein of the plants, can improve the survival ability and the photosynthesis efficiency of the plants under heat stress, and can effectively avoid and reduce adverse influence of high temperature on the plants.

The invention discloses an application of a gene CDA1 in regulation and control of chloroplast development, and belongs to the technical field of plant genetic engineering and cell biology. Accordingto the invention, an interference vector of an Arabidopsis thaliana CDA1 gene is constructed and then wild Arabidopsis thaliana is transfected to obtain an interfered strain. A research finds that cotyledons of the interfered strain show different degrees of green loss, and chloroplasts have no thylakoid membrane structure and are severe in vacuolation. According to the invention, the mutant cda1is obtained by inserting T-DNA into the Arabidopsis thaliana CDA1 gene, and the accumulation amount of photosynthetic proteins of the mutant cda1 is greatly reduced compared with the accumulation amount of photosynthetic proteins of the wild Arabidopsis thaliana. Experiments prove that the CDA1 gene participates in regulation and control of chloroplast development, influences accumulation of plantphotosynthetic protein, can be used for researching development and photosynthesis of plant chloroplasts, and provides theoretical guidance for research of development of chloroplasts of the plant and regulation and control of photosynthetic performance of the plant.