4 results about "Cytochrome b" patented technology

This invention relates to the design of specific primers for buffalo (Bubalus bubalis) cytochrome b (Cytb) gene for the detection of buffalo DNA using conventional PCR method. The primer sequence of buffalo species specific Cytb gene consists of forward sequence 5'- TTAGTACTATTCGCACCCGACCTC-3' and reverse 5'- TCGTTGTTTGGATGTATGTAGCAG-3'. This primer sequence has an amplicon size of 216 bp. The primers were used in a conventional PCR process operated under the following conditions, initiation stage of denaturation at 95ºC for 60 seconds, denaturation stage at 95ºC for 15 seconds, annealing stage at 63ºC for 15 seconds, elongation at 72ºC for 10 seconds cycle, final elongation at 72ºC for 60 seconds and at 35 cycles. This invention aims to amplify buffalo species specifically using conventional PCR methods so that it can detect the presence of buffalo DNA in processed products, especially for processed buffalo skin crackers.

This invention relates to the fields of molecular biology and forensic identification, specifically to a method for bird species identification based on multi-gene co-amplification and nanopore sequencing. The method involves extracting genomic DNA from avian biological samples; using the extracted DNA as a template, amplification is performed using four independent PCR primer pools that specifically target the avian mitochondrial cytochrome C oxidase subunit I gene, cytochrome B gene, 12S rRNA gene, and 16S rRNA gene; after purification of the amplification products, a nanopore sequencing library is constructed and sequenced; finally, bioinformatics analysis, including read length clustering, draft consensus sequence generation, and polishing, is performed on the raw sequencing data to obtain consensus sequences for each target gene. Species identification is then completed by comparing the sequence with a avian sequence database. This method effectively solves the problem that existing technologies cannot simultaneously meet the practical needs of large-scale, rapid, and high-success-rate identification of avian biological samples.

The present invention relates to the use of cytoplasmic compounds of the cytoplasmic type of formula I and N-oxides and salts thereof for combating plant pathogenic fungi which contain the amino acid substitution F129L in the cytochrome b protein of the mitochondria (also referred to as F129L mutation in the cytochrome b gene of the mitochondria) which confers resistance to Qo inhibitors and to methods for combating such fungi. The invention also relates to novel compounds, to processes for preparing these compounds, to compositions comprising at least one of the compounds and to seeds coated with at least one of the compounds.

The application relates to the field of biomaterials, and particularly relates to application of a giant salamander mucus extract hydrolysate in preparation of an anti-dentin sensitivity agent. The anti-dentin sensitivity agent comprises a hydrolysate retaining the biological activity of SSAD, the hydrolysate being a product of reduction of the giant salamander mucus extract by an acid solution with a pH value of 1-5, and the product comprising five active proteins, namely MHC class I molecule alpha chain, homeobox protein Hox-D13, abnormal heavy chain 3 of axonemal dynein, ATP synthase protein and cytochrome b; the particle size of the giant salamander mucus extract is 1-50 mu m; and the pH value of the anti-dentin sensitivity agent is 5.5-7.5. The giant salamander mucus extract is treated by the acid solution to obtain the hydrolysate containing the multiple active proteins, the hydrolysate can effectively promote deep closure of dentin tubules, and has long-term stable biocompatibility; the pH value is controlled to ensure that the biological activity is completely retained, and the curative effect of the anti-dentin sensitivity agent is remarkably improved.