6 results about "Cytochrome b" patented technology

The invention relates to a group of specific amplification primers for mitochondrial genes of procambarus clarkia and application thereof, and belongs to the technical field of molecular biology, and the mitochondrial genes of the procambarus clarkia comprise cytochrome c oxidase I subunit genes and cytochrome b genes. The specific amplification primer of the CO I gene comprises a CO I gene upstream primer and a CO I gene downstream primer, wherein the base sequences of the CO I gene upstream primer and the CO I gene downstream primer are respectively shown as SEQ NO.1 and SEQ NO.2; the specific amplification primer of the Cytb gene comprises a Cytb gene upstream primer and a Cytb gene downstream primer of which the base sequences are respectively as shown in SEQ NO.3 and SEQ NO.4. The invention further discloses a kit for detecting the Cytb gene. According to the amplification method of the mitochondrial gene of the procambarus clarkia, PCR (Polymerase Chain Reaction) amplification is carried out on a DNA (Deoxyribonucleic Acid) template solution of the procambarus clarkia by adopting amplification primers of a mitochondrial COI gene and a Cytb gene of the procambarus clarkia. The procambarus clarkia mitochondrial gene specific amplification primer has high specificity, a single target gene can be stably amplified, the length of the obtained procambarus clarkia mitochondrial COI gene segment reaches about 1000 bp, and the length of the obtained procambarus clarkia mitochondrial Cytb gene segment reaches about 800 bp.

This invention relates to the design of specific primers for buffalo (Bubalus bubalis) cytochrome b (Cytb) gene for the detection of buffalo DNA using conventional PCR method. The primer sequence of buffalo species specific Cytb gene consists of forward sequence 5'- TTAGTACTATTCGCACCCGACCTC-3' and reverse 5'- TCGTTGTTTGGATGTATGTAGCAG-3'. This primer sequence has an amplicon size of 216 bp. The primers were used in a conventional PCR process operated under the following conditions, initiation stage of denaturation at 95ºC for 60 seconds, denaturation stage at 95ºC for 15 seconds, annealing stage at 63ºC for 15 seconds, elongation at 72ºC for 10 seconds cycle, final elongation at 72ºC for 60 seconds and at 35 cycles. This invention aims to amplify buffalo species specifically using conventional PCR methods so that it can detect the presence of buffalo DNA in processed products, especially for processed buffalo skin crackers.

This invention relates to the fields of molecular biology and forensic identification, specifically to a method for bird species identification based on multi-gene co-amplification and nanopore sequencing. The method involves extracting genomic DNA from avian biological samples; using the extracted DNA as a template, amplification is performed using four independent PCR primer pools that specifically target the avian mitochondrial cytochrome C oxidase subunit I gene, cytochrome B gene, 12S rRNA gene, and 16S rRNA gene; after purification of the amplification products, a nanopore sequencing library is constructed and sequenced; finally, bioinformatics analysis, including read length clustering, draft consensus sequence generation, and polishing, is performed on the raw sequencing data to obtain consensus sequences for each target gene. Species identification is then completed by comparing the sequence with a avian sequence database. This method effectively solves the problem that existing technologies cannot simultaneously meet the practical needs of large-scale, rapid, and high-success-rate identification of avian biological samples.

The present invention relates to the use of cytoplasmic compounds of the cytoplasmic type of formula I and N-oxides and salts thereof for combating plant pathogenic fungi which contain the amino acid substitution F129L in the cytochrome b protein of the mitochondria (also referred to as F129L mutation in the cytochrome b gene of the mitochondria) which confers resistance to Qo inhibitors and to methods for combating such fungi. The invention also relates to novel compounds, to processes for preparing these compounds, to compositions comprising at least one of the compounds and to seeds coated with at least one of the compounds.

The application relates to the field of biomaterials, and particularly relates to application of a giant salamander mucus extract hydrolysate in preparation of an anti-dentin sensitivity agent. The anti-dentin sensitivity agent comprises a hydrolysate retaining the biological activity of SSAD, the hydrolysate being a product of reduction of the giant salamander mucus extract by an acid solution with a pH value of 1-5, and the product comprising five active proteins, namely MHC class I molecule alpha chain, homeobox protein Hox-D13, abnormal heavy chain 3 of axonemal dynein, ATP synthase protein and cytochrome b; the particle size of the giant salamander mucus extract is 1-50 mu m; and the pH value of the anti-dentin sensitivity agent is 5.5-7.5. The giant salamander mucus extract is treated by the acid solution to obtain the hydrolysate containing the multiple active proteins, the hydrolysate can effectively promote deep closure of dentin tubules, and has long-term stable biocompatibility; the pH value is controlled to ensure that the biological activity is completely retained, and the curative effect of the anti-dentin sensitivity agent is remarkably improved.

The invention discloses a visualized rapid detection method for solenopsis invicta, and relates to the technical field of biological detection, and the technical key points are as follows: a rapid molecular detection method for invading species solenopsis invicta is developed on the basis of a loop-mediated isothermal amplification technology, mitochondrial DNA (mitochondrial DNA, mtDNA) cytochrome b (Cytochrome b, Cytb) genes of the solenopsis invicta are taken as targets, and the primers are used for detecting the mitochondrial DNA of the solenopsis invicta and the cytochrome b of the solenopsis invicta; the method comprises the following steps: designing and screening specific LAMP primers (F3 / B3, FIP / BIP); the method can accurately distinguish the solenopsis invicta from nine related species of solenopsis invicta, breaks through the dependence of traditional molecular detection on professional equipment and complex operation, and provides a high-sensitivity and high-specificity field detection tool for solenopsis invicta invasion monitoring and port quarantine.