58 results about "Cytochrome b" patented technology

The present invention provides agents and compositions for modulating the apoptotic state of a cell. The agents comprise derivatives of antimycins which bind to an anti-apoptotic Bcl-2 family member protein. Further, the agents preferentially induce apoptosis in cells that over-express anti-apoptotic Bcl-2 family member proteins and typically exhibit reduced binding affinity for cytochrome B. Pharmaceutical uses of the agents and compositions include treating apoptosis-associated disease, such as neoplasia and drug resistance, are also disclosed.

The present invention provides agents and compositions for modulating the apoptotic state of a cell. The agents comprise derivatives of antimycins which bind to an anti-apoptotic Bcl-2 family member protein. Further, the agents preferentially induce apoptosis in cells that over-express anti-apoptotic Bcl-2 family member proteins and typically exhibit reduced binding affinity for cytochrome B. Pharmaceutical uses of the agents and compositions include treating apoptosis-associated disease, such as neoplasia and drug resistance, are also disclosed.

The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and / or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochrome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant Δ5 desaturases in oleaginous yeast, are disclosed.

The present invention relates to a PCR (polymerase chain reaction) based assay that is useful for detecting, identifying, quantitating and analysis of a target nucleic acid (target nucleic acid hereinafter) in a sample. More specifically, the present invention relates to a PCR based assay that can improve accuracy in detecting, identifying, and quantitating contamination in mammalian cell lines using a PCR-based assay of nucleic acid oligonucleotides (oligoprobes) having a sequence expected to be complementary to a target nucleic acid sequence in a sample. More specifically, the sample may contain either cells or nucleic acid isolated from a cell line. The present invention also relates to a detection kit using the PCR-based assay. The invention also involves a method for using specifically produced nucleic acids complementary to specific sequences or populations of different sequences of the cytochrome c oxidase I (COI) and / or cytochrome b, to detect, identify, and quantify specific organisms, groups of organisms, groups of eukaryotic cells or viruses in cells.

The present invention relates to one pair of PCR primers named as G-dloop, and provides one pair of amplification primers capable of amplifying the high variation zone of rockfish mitochondrial genome in high efficiency and its design method. The amplification primers consist of two single strand oligonucleotides. Through logging-on Genebank to search vertebrate mitochondrion DNA cytochrome b gene and the high conservation area of 16S rRNA gene sequence and homologous comparison, one pair of amplification primers is obtained. Through long PCR amplification, the target segments of 32 varieties of rockfish are obtained and sequenced. The obtained sequences are compared by means of using homologous comparison software Clustal X 1.83 to fine the conservation sequences in the cytochrome b5' ends and the 12S rRNA 3' ends of the mitochondrial genomes of the 32 varieties of rockfish, and the said amplification primers are designed based on the degeneracy principle.

The invention provides novel universal primers that can amplify the fragment of cytochrome b gene of any animal species in polymerase chain reaction (PCR) and reveal the identity of the biological material of any unknown animal origin and a method for identification of the specific animal from a given biological sample.

The invention discloses a method for rapidly detecting pig source components in red meat through a real-time fluorescence loop-mediated isothermal amplification (LAMP) method. According to the method, the pig specific cytochrome b genes in the red meat are detected by employing a method for combining the LAMP technology with real-time fluorescence. According to the detection method, the genome DNA of the meat to be detected is extracted, six specific primers and Bst DNA polymerase fragments with strand displacement activity are amplified at the temperature of 60-65 DEG C for 45-60 minutes, SYBR GREEN I is added into the reaction system, and the amplification conditions of the sample template can be detected in real time, so that whether pig source components are contained in beef and mutton samples can be judged. The method has the advantages of high sensitivity, high specificity, simplicity and rapidness, and the result is not required to be subjected to gel electrophoresis.

A cytochrome b-glucose dehydrogenase fusion protein having modified electron transfer properties, and a glucose measurement method and measuring kit using the cytochrome b-glucose dehydrogenase fusion protein are provided. Provided are a cytochrome b-glucose dehydrogenase fusion protein in which glucose dehydrogenase having homology with SEQ ID NO: 1 or SEQ ID NO: 4 and cytochrome b are linked together, as well as a glucose measurement method, a measurement reagent kit and a sensor using the cytochrome b-glucose dehydrogenase fusion protein. The cytochrome b-glucose dehydrogenase fusion protein of the present invention has modified electron transfer properties, and can be used for measuring glucose in the presence of a free-form mediator in reduced concentration or in the absence of a free-form mediator, and can be used, for example, in continuous glucose monitoring.

The invention discloses a puffer fish ingredient fluorescence quantitative PCR detection reagent and a preparation method and application thereof, in the technical scheme, relatively conservative cytochrome B gene fragments of different puffer fishes are selected to be the target sequences, after elaborately design and synthesis, a pair of specific primer and a specific fluorescent probe are contained, the length of the target segment is enlarged to 102bp, the sequences of the primer and the probe are respectively as follows: primer: Takifugu TF:5'-TCTTCACGAAACAGGCTCCAAC-3', Takifugu TR: 5'-GTGAAGCCCAGGAGGTCTTTGTA-3'; and probe: 5'-FAM-CGCAGACAAAATCCCATTCCACCCATA-TAMRA-3'. The invention is the fluorescence quantitative PCR detection reagent with high specificity, strong sensibility and rapid detection to the puffer fish ingredient mixed with minced fish products; and the method of the invention has reasonable design and accurate definiteness.

The invention discloses a primer for amplifying marten species cytochrome b gene and a method for identifying sable and pine marten, and relates to a primer and a species identifying method. The method solves the problem that the conventional morphologic identification method for the sable and the pine marten easily causes error judgment. The primer consists of an upstream primer and a downstream primer. The method comprises the following steps: 1, extracting DNA, and then performing PCR amplification; 2, performing enzyme digestion; and 3, performing electrophoresis detection. The skin, skeleton, muscle and claw of an animal are used as biological detection materials and then detected by using the identifying method of the invention, and the detection result is completely accurate. The method of the invention not only can be used for wild animal law enforcement, but also can be applied in commercial inspection.

The invention provides novel universal primers that can amplify the fragment of cytochrome b gene of any animal species in polymerase chain reaction (PCR) and reveal the identity of the biological material of any unknown animal origin and a method for identification of the specific animal from a given biological sample.

The invention provides a method for rapid identification of adelphocoris suturalis populations in middle area and edge area by virtue of a mitochondria molecular marker, and in particular identification of populations in Hebei and Hubei and populations in Gansu and Guizhou. According to the method disclosed by the invention, specific primers are respectively designed in accordance with three genes, namely mitochondria cytochrome oxidase I (COI), cytochrome b (Cytb) and mitochondria NADH dehydrogenase 5 (ND5), and PCR amplification products are subject to serial comparison, so that identification of the adelphocoris suturalis populations in middle area and edge area of China is achieved. The method is simple and reliable, strong in operability, good in stability and strong in repeatability.

The invention discloses a method for identifying the traditional Chinese medical material corn cervi pantotrichum based on PCR-RFLP. A reagent kit used for identifying the traditional Chinese medical material corn cervi pantotrichum comprises a primer pair shown in (a) or (b) and restriction enzyme DdeI or isoschizomers of the restriction enzyme DdeI, wherein (a) is the primer pair composed of two lines of single-stranded DNA molecules shown in a sequence 1 and a sequence 2 in a sequence table, (b) is the primer pair which is composed of two lines of single-stranded DNA molecules shown in the sequence obtained by replacing and / or deleting and / or adding one or several nucleotides shown in the sequence 1 and the sequence 2 in the sequence table and is identical with the primer pair in (a) in function. According to the method, cytochrome b gene sequences of the traditional Chinese medical material corn cervi pantotrichum and counterfeit drug of the traditional Chinese medical material corn cervi pantotrichum are analyzed, different SNP loci of the traditional Chinese medical material corn cervi pantotrichum and the counterfeit drug of the traditional Chinese medical material corn cervi pantotrichum are obtained, identification primers and the restriction enzyme are designed, and the traditional Chinese medical material corn cervi pantotrichum and the counterfeit drug of the traditional Chinese medical material corn cervi pantotrichum are accurately identified through the restriction fragment length polymorohism polymerase chain reaction technology.

The invention discloses PCR detection primers for seven different species of cells such as MDBK, MDCK, BHK21, ST, SP20, 293 and VERO commonly used in a laboratory and a detection method and use thereof. The detection primers comprise seven pairs of primers, each pair of the primers only produce specific amplification effects on a cytochrome b (Cytb) gene fragment of the corresponding specie of cells so that the primers can synchronously detect seven different species of cells, have high specificity, realize identification of specie sources (ie. cell source reliability) of cells commonly used in a laboratory, realize determination of cross contamination of different species of cells in cell culture and provides a scientific basis for cell culture and subsequent experiment smooth implementation. The PCR detection primers have the advantages of good specificity, high sensitivity, fastness and simpleness.

The invention provides a method for rapidly identifying Lygus pratensis populations, especially Xinjiang populations, Gansu populations and Ningxia populations, by mitochondrial molecular markers. According to the method, specific primers are designed according to four gene segments including mitochondrial cytochrome oxidase II (COII), cytochrome b (Cytb), mitochondrial NADH dehydrogenase subunit 5(ND5) and 16S rDNA of Lygus pratensis respectively, and PCR (polymerase chain reaction) amplification products are subjected to series-connection comparison, so that identification for the Lygus pratensis populations in China is realized. The method is simple and reliable and has high operability, good stability and high repeatability.

The invention provides a CYBB (cytochrome B-245 beta chain) lentiviral vector, lentiviral vector-transfected stem cells and a preparation method and application of the lentiviral vector-transfected stem cells. The lentiviral vector comprises a hEF1alpha promoter and CYBB tandem co-expression. The lentiviral vector carries CYBB gene; under promotion of the hEF1alpha promoter, the carried CYBB genecan be expressed safely and efficiently differentiated or non-differentiated stem cells; in addition, the stems can act as a latent transport vector, and the expression quantity of the CYBB gene in transgenic cells is increased.

The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and / or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochrome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant Δ5 desaturases in oleaginous yeast, are disclosed.

The invention provides a method for accurately and quickly identifying two morphological variants of marsupenaeus japonicus, which relates to the marsupenaeus japonicus. The method comprises the following steps of: designing and amplifying primers of cytochrome b gene segments of the marsupenaeus japonicus; and extracting the DNA of the variants I and II of the marsupenaeus japonicus, performing polymerase chain reaction (PCR) by taking the DNA as a template, performing enzyme cutting on PCR products and performing electrophoresis identification on an enzyme-cutting products, wherein the variant I of the marsupenaeus japonicus has the enzyme cutting sites of gatatc and produces the two enzyme-cutting segments of 284bp and 299bp by the enzyme cutting; and the variant II of the marsupenaeus japonicus does not have any enzyme-cutting site and still has the strip of 583bp, so that the two variants of the marsupenaeus japonicus can be identified.

The invention relates to a method for detecting the polymorphism of 12 loci of human mitochondrial cytochrome b genes simultaneously, and provides a method for detecting the polymorphism of 12 loci of mitochondrial cytochrome b genes (Cytochrome b, Cytb) simultaneously in the same system, which realizes the detection of the polymorphism of the 12 loci of the cytochrome cytb genes simultaneously in the same reaction system by combining the technology of polymerase chain reaction (PCR) with the technology of single basic group extension and fluorescence microarray hybridization. The fluorescence intensity and fluorescence type which are generated by single basic group extension only correspond to a genotype, and a polymorphic locus has only three kinds of characteristic fluorescence at most, and thus the genotypes of samples corresponding to all the same peak types can be obtained only by one-time DNA sequencing verification. Compared with a detection method by direct sequencing, the method of the invention has short time, namely experiments are finished within one day, and has high flux, namely the 12 polymorphic loci are detected directly in the same reaction; in addition, the method has low cost and visual result judgment.

The invention relates to a PCR amplification primer for a chondriosome cytb gene segment of Marsupenaeus japonicus and an identification method thereof, belonging to the field of shrimps population identification. The invention provides a pair of PCR amplification primers for identifying the geographic group of the Marsupenaeus japonicus, respectively containing the nucleotide sequences of L10945:5'-TGAGGAGGTTTCGCAGTA-3' or H11563: 5'-AGATGAGGGTGAGTGGGT-3'. The identification method comprises the steps: firstly, carrying out PCR amplification on a cytochrome b gene segment in the chondriosome DNA of a sample of four geographic groups to be detected by using the pair of amplification primers; and secondly, purifying the amplification primer and sequencing, wherein a sample to be detected with the 528-locus and the 534-locus bases of the nucleotide sequences to G is from Fujian group. The invention has the characteristics of stable amplification result, favorable repeatability and simple and effective identification method.

The invention provides a primer and a method for molecular identification of channel catfish and leiocassis longirostris. The nucleotide sequence of the primer is shown as SEQ NO: 1 and SEQ NO: 2 or SEQ NO: 3 and SEQ NO: 4. Similar fishes such as channel catfish are identified from the cytochrome oxidase subunit I gene and the cytochrome b gene, and the two fishes can be rapidly, conveniently, accurately and reliably identified by utilizing the primers through a mitochondrial genome DNA bar code technology; the primer and the method can quickly, conveniently, accurately and reliably identify channel catfish, ietalurus punetaus and the like, are good in result stability and high in repetition rate, fill the blank of identifying channel catalurus punetaus, ietalurus punetaus and pelteobagrus fulvidraco according to domestic molecular biological standards at present, play an important role in ecological resource investigation and fish variety identification, and have good application prospects. Wide application prospects are realized.

Provided herein is a method for screening a modulator of apoptosis by contacting a cell comprising a FAS mediated apoptosis system, major vault protein and cytochrome b, with a candidate modulator, and measuring the level of apoptosis of the cell. The modulator of apoptosis is identified by a change in apoptosis in comparison to a control.

The invention discloses a durable graphene antivirus antibacterial liquid and a preparation method thereof. The antibacterial liquid is prepared from the following raw materials in parts by weight: 3-8 parts of graphene oxide, 10-15 parts of an antibacterial synergist, 3.5-5 parts of sodium dodecyl benzene sulfonate, 3-5 parts of an emulsifier, 1-3 parts of glycerol, 1-3 parts of propylene glycol and 80-100 parts of water. The antibacterial synergist is prepared in the process of preparing the antibacterial liquid, the antibacterial synergist can act with a head group of acidic phospholipid in a bacterial cell membrane, so the infiltration capacity of the cell membrane is reduced, bacterial cell sap leaks, and bacterial cells die; meanwhile, the synergist can be combined with a Q0 site of cytochrome b in bacterial mitochondria, and electron transfer between the cytochrome b and the cytochrome c1 is blocked, so mitochondrial respiration is inhibited, mitochondria cannot supply energy required by normal metabolism of cells, and sterilization is more thorough.

The DNA probes produced by molecular cloning and the characterization of specific gene region sequences is provided, these can be used as genetic markers for the genes such as Cytochrome b (cyt b); Mitochondrial control region (D-Loop); Inter Transcribed Spacers (ITS2) and Rhodopsin (ROD), 12S rRNA and 16S rRNA in mesopelagic lantern fishes which are found in the mesopelagic zones of the oceans where the photic regime is of dim light and associate themselves with the oxygen minimum layer, it also includes the recombinant DNA techniques for the preparation of specific gene probes and sequences of species specific primers of lantern fishes, novel gene probes and novel oligonucleotides for amplification of myctophid genes are disclosed.

The present invention discloses a RFQ-PCR detection method of skeletonema costatum cytochrome b gene. Said method includes the following steps: (a), extracting skeletonema costatum RNA as standard sample; (b), separating and cloning skeletonema costatum cytochrome b gene to obtain skeletonema costatum cytochrome b gene sequence, and obtaining cytochrome b sequences of several algal seeds which are approaching to the skeletonema costatum in status from GenBank, and constructing system tree; (c), making RFQ-PCR; and (d) utilizing obtained skeletonema costatum cytochrome b gene sequence with 700-740bp to construct standard curve and utilizing said standard curve to detect the skeletonema costatum cytochrome b gene to be detected.

The present invention relates to one pair of PCR primers named as G-dloop, and provides one pair of amplification primers capable of amplifying the high variation zone of rockfish mitochondrial genome in high efficiency and its design method. The amplification primers consist of two single strand oligonucleotides. Through logging-on Genebank to search vertebrate mitochondrion DNA cytochrome b gene and the high conservation area of 16S rRNA gene sequence and homologous comparison, one pair of amplification primers is obtained. Through long PCR amplification, the target segments of 32 varietiesof rockfish are obtained and sequenced. The obtained sequences are compared by means of using homologous comparison software Clustal X 1.83 to fine the conservation sequences in the cytochrome b5' ends and the 12S rRNA 3' ends of the mitochondrial genomes of the 32 varieties of rockfish, and the said amplification primers are designed based on the degeneracy principle.

The invention provides a fluorogenic quantitative PCR specific primer, a probe and a kit of the primer and relates to the technical field of food check and molecular biological detection. The primer, the probe combination, real-time fluorescence PCR and the detection kit are utilized and combined, and therefore the camel source ingredients contained in meat products can be identified fast. The real-time fluorescence quantitative PCR technology and the fluorescence probe nucleic acid DNA molecular marker technology are applied, the camel source specific primer and the specific probe are designed according to the mitochondrion cytochrome b (Cytb) gene height keeping area, and the primer has the advantages of being high in sensitivity, good in accuracy, fast, small in degradation degree (mtDNA is kept completely in the processing process), stable and easy to operate.

An economical process for in vivo production of the pigment astaxanthin, and particularly a process for enhancing astaxanthin content of cultures of microorganisms of genus Phaffia, the process comprising culturing a microorganism of genus Phaffia in a nutrient medium containing an antibiotic, a cytochrome B inhibitor, or a terpenoid synthetic pathway inhibitor, cultivating surviving pigment enhanced microorganisms, and harvesting the yeast.

The invention relates to the technical field of food detection, in particular to a multiplex PCR detection kit for mouse meat, goat meat and mutton, which is characterized by comprising an animal tissue mitochondrial DNA extraction system and three multiplex PCR reaction systems (a positive control reaction system, a negative control reaction system and an identification reaction system). The total PCR reaction system is 50 microliters, containing 5 microliters of reaction buffer solution, 2-5 microliters of dNTP, 23 microliters of MgCl, 1-5 microliters of Taq DNA polymerase, 0.5-2 microlitersof each primer, 1-3 microliters of DNA of a sample to be detected, and the balance being double distilled water. A multiplex PCR identification method for the mouse meat, the goat meat and the muttoncomprises the steps of designing three specific oligonucleotide primers by utilizing species specificity of cytochrome b, determining a reaction procedure, judging results and the like; the multiplexPCR identification method can simultaneously distinguish the specificity of the mouse meat, the goat meat and the mutton; and the multiplex PCR identification method for the mouse meat, the goat meatand the mutton has the advantages of simplicity, convenience, rapidness, reliable detection result and the like.