213 results about "Cytochrome c" patented technology

The present invention relates to cupredoxin and cytochrome and their use, separately or together, to inhibit the spread of parasitemia in mammalian red blood cells and other tissues infected by the malaria parasite, and in particular the parasitemia of human red blood cells by P. falciparum. The invention provides isolated peptides that are variants, derivatives or structural equivalents of cupredoxins or cytochrome c, and compositions comprising cupredoxins and / or cytochrome c, or variants, derivatives or structural equivalents thereof, that are useful for treating or preventing malaria infection in mammals. Further, the invention provides methods to treat mammalian patients to prevent or inhibit the growth of malarial infection in mammals. The invention also provides methods to prevent the growth of malaria infection in insect vectors.

The present invention relates to cupredoxin, specifically Pseudomonas aeruginosa azurin, and / or Pseudomonas aeruginosa cytochrome C551 and their use in inhibiting of viral infection, and in particular infection of mammalian cells by the Human Immunodeficiency Virus (HIV). The invention also relates to variants and derivatives of cupredoxin and cytochrome c that retain the ability to inhibit viral infection, and in particular infection by the Human Immunodeficiency Virus (HIV). The invention also relates to research methods for studying viral and bacterial infection in mammalian cells.

The invention discloses a method for preparing a meso-porous silicon nano medicine carrier with cell specificity target, reduction responsiveness and triple anticancer treatment effects. The method comprises the following steps: firstly, synthesizing meso-porous silicon nano particles by using a gel dissolution method, subsequently, introducing a disulfide bond onto the surface of a meso-porous silicon nano reservoir by using a chemical modification method, innovatively fixing cytochrome C with an apoptosis-inducing function onto the surface of the meso-porous silicon nano reservoir, blocking meso-porous channels with medicines, finally modifying DNA (Deoxyribose Nucleic Acid) aptamer single chain molecules (AS1411, with a cancer cell apoptosis-inducing function) onto the surface of a meso-porous silicon / cytochrome C nano composite system, and taking the system as specificity ligand of a receptor (nucleolin protein) which is overexpressed on the surface of liver cancer cytomembrane, thereby establishing a multifunctional composite type nano medicine carrier system for achieving triple anticancer treatment under combined action of medicines, blocking substances and target molecules inside meso-pores.

The invention provides two preparation methods of a high-water-stability conductive poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) composite electrode. One preparation method comprises the steps of adding PEDOT:PSS into a polyvinyl alcohol solution, directly drippingly coating the mixed solution on the electrode surface, or adding multi-wall carbon nanotubes, carboxyl functionalized graphene and cytochrome c into the mixed solution for further modification to obtain the PEDOT:PSS composite electrode having excellent properties; and the other preparation method comprises the steps of grafting acrylic acid on EDOT, carrying out copolymerization of EDOT and EDOT-AA, dispersing the obtained copolymer into a sodium polystyrenesulfonate solution, and directly drippingly coating the P(EDOT:EDOT-AA):PSS dispersion liquid onto the electrode surface. The preparation methods solve the problems that the PEDOT:PSS composite electrode is quite easily expanded, absorbs moisture and is degraded in a water solution; and the prepared composite electrode has the advantages of excellent water stability, low preparation cost, simple process and the like.

The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using assays that detect one or more markers selected from the group consisting of cytochrome c and insulin-like growth factor IA as diagnostic and prognostic biomarkers in renal injuries.

Disclosed is an enzyme electrode having an oxidoreductase (for instance, glucose oxidase, cholesterol oxidase, fructosylamine oxidase and glucose dehydrogenase) and an electron-transfer protein (for instance, cytochrome C, cytochrome b562 and cytochrome c551), as well as a sensor utilizing the enzyme electrode as working electrode. The enzyme electrode of the invention can provide high response current values.

The present invention relates generally to assays and methods involving Cytochrome c (Cyt c) and leucine-rich alpha-2-glycoprotein-1 (LRG), and related antibodies. In an embodiment, the invention includes a method of detecting LRG in a sample, the method including disposing Cyt c on a substrate; contacting the sample with the Cyt c; contacting bound components of the sample with an antibody or antibody fragment specific for LRG; and quantitating the amount of the antibody or antibody fragment bound to LRG. In an embodiment, the invention includes a method of purifying or enhancing the purity of LRG from a sample, the method including contacting the sample with Cyt c; forming a complex between LRG in the sample and Cyt c; removing uncomplexed components of the sample; releasing LRG from the complex with Cyt c; and collecting the released LRG. In an embodiment, the invention includes an isolated antibody produced by a hybridoma cell line (ATCC Accession Number PTA-8131), or antibody fragment thereof that specifically binds to LRG. In an embodiment, the invention includes a kit comprising an antibody that specifically binds to LRG, or a fragment thereof that specifically binds to LRG, and a compartment, wherein the antibody or fragment is contained within the compartment. Other embodiments are described herein.

The invention provides a preparation method of a Bi2Te3 dimensional nano tablet. The preparation method comprises a step of subjecting a Bi2Te3 nano material, water, and functional ligand molecules to ultrasonic exfoliation so as to obtain the Bi2Te3 dimensional nano tablet; wherein the functional ligand molecule is polyvinylpyrrolidone, cytochrome C, or bovine serum albumin. In the preparation method, the functional ligand molecule is taken as the stripping agent to carry out ultrasonic exfoliation on a Bi2Te3 nano material so as to obtain a functional ligand molecule-Bi2Te3 dimensional nano tablet with high water solubility; and the dimensional nano tablet has the advantages of uniform particle size, controllable morphology, high water solubility, good biocompatibility, and high photo-thermal conversion efficiency, is a good photo-thermal agent, and can be applied to tumor photo-thermal therapy. The provided preparation method can synthesize massive water-soluble Bi2Te3 with a photo-thermal property, the preparation process is simple, the cost is low, and the preparation method is suitable for industrial production.

The invention discloses an enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor and a preparation method thereof. A denovo method is used for citric acid modification on the surface of the Fe3O4 magnetic nanometer particles; then, ZIF-8 is coated to form a ZIF-8@ZIF-8@Fe3O4 core-shell structure; meanwhile, chloroperoxidase, horse radish peroxidase or cytochrome C are immobilized onto ZIF-8 in the ZIF-8 coating process, so that the enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor with the magnetic separation effect is obtained, wherein the particle diameter of the citric acid modified Fe3O4 magnetic nanometer particles is 250 to 300 nm; the thickness of the ZIF-8 shells of the surface coated immobilization enzyme is 50 to 80 nm. The preparation method provided by the invention is simple and convenient; the thermal stability and the acid and base stability of the obtained enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor are obviously improved through being compared with that of free enzyme; good repeated use performance is realized.

Live, attenuated recombinant rabies virus vaccines are generated using reverse genetics to combine the antigenic determinants that render the rabies virus non-pathogenic with the determinants that are responsible for the elicitation of an effective anti-rabies immune response. These vaccines do not affect the antigenic, and therefore the immunogenic, properties of the virus. The present invention further relates to recombinant rabies virus vaccines that express a pro-apoptotic protein, such as cytochrome c, to increase the capacity to induce apoptosis, thereby enhancing the protective immunity against rabies. This new generation of live rabies virus vaccines represents a safe and effective approach to the eradication of rabies in wildlife, and subsequently humans and livestock.

The invention provides a method for preparing H2O2 biosensor of MWNTs-IL / Cyt c / GCE, which includes the following steps: performing chemical reaction between a multi-wall carbon nano tube subjected to carboxylation and ionic liquid subjected to amidation, so as to form a carbon nano tube subjected to amidation; and then fixing a cytochrome c on a glassy carbon electrode modified by the carbon nanotube subjected to amidation to increase the adsorption and catalytic activity of the cytochrome c. Through adopting the method, the detection line of the hydrogen peroxide obtained from the ampere response can reach 1.3*10<-8>M, and the MWNTs-IL / Cyt c / GCE has sensitive response to the oxidation of the hydrogen peroxide.

The present invention relates to a technique for measuring a glucose level by utilizing a reaction system containing an enzyme and an electron carrier. In accordance with the glucose level measuring method of the present invention, glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier. The present invention further provides a glucose sensor in which glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier.

The invention discloses a method for detecting cytochrome c in a living cell based on a Raman-fluorescent dual-mode probe. The detecting method realizes dual-signal detection based on on-off conversion of Raman scattering and fluorescence resonance energy transfer through a nano-sensor which is constructed through assembling an adapter of the cytochrome c(Cyt c) on a gold triangle sheet (AuNTs). The method of the invention can simultaneously detect the Cyt c in the living cell through a Raman signal and a fluorescent signal. Furthermore the method has advantages of high detection singularity,high sensitivity and high accuracy. Furthermore compared with ELISA, the error is lower than 5%.

A high-sensitivity analyzer for nitric oxide in exhaled breath at levels of 200 ppb or less with a sensor containing cytochrome C is rendered capable of multiple uses without the need for installing a new sensor for each use. This capability is achieved by regenerating the analyzer after each use by purging the sensor and surrounding regions with NOx-free air in a controlled manner, preferably in pulses separated by equilibration periods.

The subject invention concerns N-thiolated β-lactam compounds of formula A, wherein R1is a hydrocarbon group having 1-8 carbon atoms; R3 is an organothio group; and R4 is alkyl, alkenyl, alkynyl, aryl, heteroaryl, cycloalkyl, heterocycloalkyl, cycloalkenyl, or heterocycloalkenyl, and analogs and pharmaceutically acceptable salts, esters and amides thereof. The subject invention also concerns methods for inducing tumor cell death or inhibiting tumor cell proliferation, and methods for inducing DNA damage, inhibiting DNA replication, activating p38 MAP kinase, or activating caspase cascade activation, or releasing cytochrome C from mitochondria into the cytoplasm in a tumor cell. Methods for treating cancer using N-thiolated β-lactam compounds, as well as pharmaceutical compositions comprising the same are further disclosed.

Disclosed is a preparation method of an artemisinin molecular imprinted photoelectrochemical sensor. The invention discloses the preparation method of an artemisinin molecular imprinted photoelectrochemical sensor based on the action of cytochrome C and pyronine B. The preparation method comprises the following steps: 1) performing electrochemical polymerization on a glassy carbon electrode to prepare a molecular imprinted polymer film and performing elution to remove template molecules and obtain imprinted holes; 2) immersing imprinted polymer in an artemisinin-contained solution to shelter holes in the imprinted film, putting the obtained product in a solution containing cytochrome-C-marked template molecules for replacing the template molecules in the imprinted holes and causing a competitive reaction, and putting the obtained product in a potassium-ferricyanide-contained electrolyte solution to observe the change of electrical signals; and 3) achieving photoelectrochemical detection of artemisinin with decrease of the cytochrome-C-marked template molecules in the solution and reduction of the fluorescence of pyronine B taken as a substrate. The obtained artemisinin molecular imprinted sensor has advantages of simple operation, high selectivity, and low cost. Combination of optical and electrical signals is adopted, and the sensor can be used as a novel molecular imprinted sensor used for efficient detection of artemisinin in a biological sample and a complex system.

The invention discloses a protein and amorphous metal organic framework compound and a preparation method thereof. The organic framework compound has a 2 nm-50 nm mesoporous structure. The preparationmethod comprises the following step of enabling a protein, zinc ions and an organic ligand to react in a solvent, wherein the organic ligand is a compound containing an imidazole group; the protein is one or a combination of several kinds of cytochrome C, cytochrome P450, horse radish peroxidase, alcohol dehydrogenase, lipase, acetylcholin esterase, laccase, a green fluorescent protein, glucose dehydrogenase, glucose oxidase, trypsin, bacillus subtilis protease, carbonic anhydrase, aldehyde ketone reductase, amylase, saccharase, superoxide dismutase, urease and catalase. The preparation method of the protein and amorphous metal organic framework compound provided by the invention is simple to operate and mild in condition, the obtained product is high in protein embedding rate and good inprotein stability, and the biologic al activity of the protein is reserved to a greater extent.

The invention relates to a cell apoptosis double-color detection and imaging probe and application thereof. The probe adopts a fluorescently-labeled cytochrome c nucleic acid aptamer and a caspase-3 substrate sequence polypeptide sequence as recognition elements and golden nanoparticles as a fluorescent energy receptor, response molecules are subjected to covalent modification on the surface of the receptor, and then fluorescence of two fluorescent dyes is quenched off in the energy transfer process simultaneously. When no target is available, the dye fluorescence is quenched off. When target molecules are available, the cytochrome c is combined with the aptamer, a substrate polypeptide of the cytochrome c is hydrolyzed with caspase-3, then the fluorescent dye is far away from the surfaces of golden nanoparticles, a fluorescence signal is generated, and detection and imaging of cytochrome c and caspase-3 are achieved. The probe provided by the invention is simple and convenient to synthesize, high in sensitivity and good in selectivity; two biomarkers related to cell apoptosis can be detected at the same time; and the probe is effectively applied to cell apoptosis imaging detection and cell apoptosis medicine evaluation.

The invention provides a sensing material based on an up-conversion nanoparticle. The sensing material comprises cytochrome C and polyacrylic acid modified up-conversion nano-particles (UCNPs). The invention also discloses a preparation method of the sensing material. The sensing material has the advantages that the polyacrylic acid modified UCNPs are introduced to be used as a carrier for providing a relatively-large surface area and are also introduced to be used as a fluorescent material for providing a fluorescent signal. The molecularly-imprinted sensing material synthesized by using a sol-gel method is small and uniform in particle size and has relatively high selectivity for cytochrome C. The sensing material combines strong fluorescence of the UCNPs and the specificity recognition of a molecularly-imprinted polymer so as to be wide in application prospect.

A precursor medicine targetting to kidney is composed of the low-molecular protein chosen from lysozyme, insulin, cytochrome C and pepetide suppressing enzyme or its polypeptide fragment, and the extract of common threewingnut root or its derivative, or glucocorticoid. It can be used as the immune depressant for treating renal disease, or the medicines for treating the rejection reaction and nephritis.

The disclosure discloses an enzyme electrode comprising; an electrode comprising a current collector; a monolayer-forming molecule bound to the surface of the current collector; and a glucose dehydrogenase comprising a cytochrome C subunit bound to the monolayer-forming molecule; wherein electrons are transferred between the glucose dehydrogenase and the current collector by oxidation-reduction reaction of the glucose dehydrogenase.

The present invention relates to a protein-immobilized membrane (14) including a cell membrane homologous layer (14A) and a protein (14B) immobilized to the cell membrane homologous layer (14A), where the protein contains cytochrome or a cytochrome complex. The present invention also relates to a method for forming a protein-immobilized membrane (14), and an enzyme-immobilized electrode and a biosensor (X1) provided with a protein-immobilized membrane (14). Preferably, the cell membrane homologous layer (14A) may contain a phospholipid polymer, and the protein (14B) may be CyGDH including an α subunit having a glucose dehydrogenase activity and cytochrome C having a function of electron transfer.

The present invention relates to nanometer cytochrome C liposome medicine and its preparation process. The nanometer cytochrome liposome medicine consists of neutral phosphatide 40-80 weight portions, sitosterol 10-20 weight portions, cholesterol 20-40 weight portions, cytochrome C 10-30 weight portions, polyglycol 20-25 weight portions, reductive glutathione 5-10 weight portions, and vitamin C 40-50 weight portions. The nanometer cytochrome liposome medicine is used in the first aid and auxiliary treatment of anoxia clinically.

The invention belongs to technology of qualitative detection of animal-derived components in food and feed, and particularly relates to a primer / probe group for detecting fox component in food and feed. The invention selects mitochondrion cytochrome C redox enzyme I subunit gene sequence of fox, and designs a group of specific primers and probes. After using the primers and probes, the real-time fluorescent PCR (polymerase chain reaction) technology can be adopted to quickly, sensitively and specifically detect the fox component in food and feed. The primers and probes can also be provided together with other reagents in the form of a kit, and is used for nucleic acid amplification reaction. The method is simple to operate and has favorable repetitiveness.

The invention discloses a nitrogen-doped graphene quantum dot, a preparation method and applications thereof, and belongs to the technical field of fluorescent nanometer materials. The preparation method comprises: carrying out a hydrothermal reaction on an aminopyrene-containing aqueous solution under an alkaline condition to prepare the nitrogen-doped graphene quantum dot. According to the invention, the graphene quantum dots with blue fluorescence are prepared in the alkaline solution through the one-step hydrothermal method by simultaneously using aminopyrene as the carbon source and the nitrogen source, so that the preparation method is simple and easy to operate, the yield is high, and the fluorescence quantum yield of the product is high; the synthesized nitrogen-doped graphene quantum dot has 2-3 layers of graphene thickness, is uniform in size, and has single crystallinity; the fluorescence of the product can be specifically quenched by iron ions and cytochrome C so as to be expected to be used for selectively detecting trace iron ions or cytochrome C; and the nitrogen-doped graphene quantum dot synthesized by the method has electrochemical luminescence property, and is expected to construct a high-sensitivity electrochemical sensing system.

The present invention relates to a lyophilized preparation of a cytochrome C-containing pharmaceutical composition for injection, and the lyophilized preparation comprises the following components by weight: 75-300 parts of cytochrome C; 30-60 parts of L-arginine; 2-5 parts of disodium edetate; and 150 parts of mannitol. In the lyophilized preparation, disodium edetate and L-arginine are selectd as excipients, thereby helping to improve the stability of cytochrome C; in the preparation process of the liquid pharmaceutical composition, mannitol is used as a lyophilizing excipient, and phosphoric acid or a sodium phosphate solution is used as an pH adjusting agent, thereby enabling the product quality more stable; and after a mannitol solution is treated activated carbon, the cytochrome C is added, thereby preventing the problem of decrease in the cytochrome C level due to the adsorption by activated carbon. The preparation method of the present invention shortens the lyophilized time from 23 hours to 14 to 16 hours, and reduces energy consumption by 30%; while the production capacity is improved by about 30% without increasing hardware input, thereby significantly reducing the cost of production.