22 results about "Cytochrome c" patented technology

The invention provides a biological sewage treatment accelerant as well as a preparation method and application thereof, and belongs to the technical field of sewage treatment. According to the application, the fruit and vegetable fermentation liquor, the inorganic carbon source, the lipase, the protease, the amylase, the cellulase, the B vitamins, the cytochrome c, the coenzyme Q and the ethylene glycol are used as main components to prepare the accelerant, the accelerant is added into a biological sewage treatment reaction tank, and the biological sewage treatment effect is enhanced by increasing the microbial nitration reaction and denitrification reaction rate.

The present disclosure is directed to compositions comprising mitochondrial optogenetics-based gene therapies and their methods of use. In some embodiments, a composition described herein comprises an expression vector comprising a first nucleic acid sequence encoding a channelrhodopsin fusion protein and a second nucleic acid sequence encoding a luciferase protein. In some cases, the first nucleic acid sequence and the second nucleic acid sequence are operably linked to an expression control sequence. In some instances, the channelrhodopsin fusion protein comprises a channelrhodopsin protein linked to an inner mitochondrial membrane-mitochondrial localization signal (IMM-MLS). In some implementations, when the expression vector is expressed, the luciferase protein is localized to the cytosol. In some cases, the IMM-MLS comprises a leading sequence from a mitochondrial inner membrane protein selected from ABCB10, ABCB140, Cytochrome C, and renal outer medullary potassium channel (ROMK).

The invention belongs to the technical field of enzyme composite materials and biological catalysis, and particularly relates to an immobilized enzyme catalyst based on a copper-based metal organic framework as well as a preparation method and application of the immobilized enzyme catalyst. Cytochrome c (Cyt c), squaric acid and copper ions are uniformly mixed through an in-situ co-precipitation method, in-situ embedding of Cyt c is achieved through hydrogen bonds and coordinate bonds generated in the Cu-MOF forming process, the Cyt c-coated Cu-MOF compound enzyme catalyst is prepared, a constructed Cu-MOF carrier is of a mesoporous structure, a stable protective microenvironment can be provided for Cyt c, enzyme leaching is effectively inhibited, and the catalyst has the advantages of being capable of being used for preparing the complex enzyme catalyst. The operation stability and the environmental tolerance are remarkably improved, and the catalyst shows efficient catalytic degradability in organic pollutants including phenolic compounds and dyes, and has wide application prospects in the fields of biological catalysis and the like. Moreover, the method is mild in condition, simple and convenient to operate, low in cost and easy for large-scale production.

This invention discloses a method and its application for isolating mitochondria from mesenchymal stem cells (MSCs) to improve their yield, purity, and activity. Compared to existing methods for isolating MSCs, the method of this invention effectively improves the yield and purity of MSCs, increasing the number of mitochondria by 2.96 times. The method also better maintains the activity of MSC mitochondria, significantly increasing cytochrome C content and exhibiting superior activity in treating autoimmune hepatitis. Furthermore, the method is simple, requires minimal reagents, and has broad application prospects in the field of mitochondrial transplantation therapy.

The invention discloses a sensor for detecting cytochrome c and caspase in a single cell as well as a preparation method and application of the sensor. The sensor comprises a theta-type nanotube with two channels, wherein a cytochrome c aptamer and a caspase recognition peptide are respectively fixed on the two channels; one end of the theta-shaped nanotube is a tip, is provided with a theta-shaped nanopore and is used for puncturing into a single cell for sampling. Different detection molecules are respectively fixed in two channels of the theta-type nanotube, and synchronous, high-sensitivity and high-selectivity detection of two key apoptosis markers in a single cell is realized by utilizing the change of charge density on the inner wall of the channel when a target exists. The sensor has good reversibility, stability and reproducibility, can be used for monitoring dynamic changes of cyt c release and caspase-3 activation in a cell apoptosis process in real time, reveals spatial-temporal heterogeneity of an apoptosis mechanism, and provides a powerful tool for cell apoptosis research, disease diagnosis and treatment evaluation.

The invention discloses a high-performance tissue sealant as well as a preparation method and application thereof, and relates to the technical field of medical materials. The tissue sealant is prepared from the following raw materials: amino-containing protein, a multi-arm polyethylene glycol derivative and an optional multi-amino polymer, wherein the amino-containing protein comprises at least one of lactoferrin, phycocyanin, whey protein, beta-lactoglobulin, cytochrome C, recombinant collagen, recombinant fibronectin and alpha-lactalbumin. The sealant provided by the invention has the characteristics of rapid gelling, strong adhesion, high mechanical strength, low swelling, antibacterial property and the like, can realize efficient tissue wound sealing, hemostasis and repair in a wet complex environment, is simple and convenient to prepare, safe in material source and good in biocompatibility, is suitable for being used in various wound scenes, and has wide application prospects. Good application prospects are realized.

The invention relates to application of beauveria bassiana in preparation of a medicine for treating multiple myeloma. The invention shows that the Beauverin has a remarkable effect of resisting multiple myeloma, and the action mechanism of the Beauverin is that the expression of anti-apoptotic protein Bcl-2 is inhibited, the expression of pro-apoptotic protein Bax is promoted, the release of cytochrome C is promoted, the activation of Caspase proteases such as Caspase 3 and the like is caused, and finally the apoptosis of cells is caused. Beauveria bassiana regulates an apoptosis pathway through multiple targets, plays a role in resisting multiple myeloma, and provides an experimental basis for developing a novel treatment strategy. Compared with the prior art, the specific action mechanism of the beauveria bassiana is defined, and the value of the beauveria bassiana as a potential anti-tumor drug is highlighted.

The application discloses a kind of electroactive microorganism detection method and kit.The application utilizes the catalytic action of electroactive microorganism extracellular electron transfer key protein (outer membrane cytochrome c) and chemiluminescence technology, combined with the photographing function of smart phone, picture information processing technology, provide a kind of electroactive microorganism detection method and detection kit.No need to use any nanomaterial or special equipment, by commonly used smart phone and computer application program can realize signal acquisition, processing, calculation and result output;Convenient and fast, good stability, low cost, simple operation, the whole detection process can be completed without professional training;And response speed is fast, the whole detection process can be completed in 2min;It can be widely used in field detection.

The present application relates to the technical field of nanocomposite, and particularly relates to a photocatalytic nanocomposite, a preparation method and application thereof. The preparation method comprises three steps of molybdenum disulfide nanoparticle preparation, precursor dispersion and in-situ compounding, and has the advantages of simple process, low energy consumption and high production efficiency, and can realize efficient and low-cost large-scale preparation of the photocatalytic nanocomposite. The photocatalytic nanocomposite is composed of two kinds of semiconductor photocatalytic materials with staggered energy bands, can not only adjust the light absorption range, but also realize effective separation of photo-generated electron-hole pairs by transferring photo-generated electrons and holes to different semiconductor photocatalytic materials. The photo-generated electrons and holes generated by light excitation can make the composite material have strong oxidation-reduction capacity, and can be used for preparing products for adjusting mitochondrial function, so as to accurately interfere with the mitochondrial electron transport chain and realize oxidation of NADH and reduction of cytochrome C.

The present invention discloses a method for precision engineering of protein bioconjugates by harnessing complex multi-equilibria systems. The method allows for exclusive control over reaction pathways, achieving highly selective outcomes, even with complex substrates such as proteins. By manipulating proteinogenic functionalities, the invention enables pathway selectivity, chemoselectivity, and site selectivity, leading to single-site modifications in proteins. The versatility of the present invention is demonstrated through diverse protein examples, including insulin, RNase A, ubiquitin, cytochrome C, myoglobin, α-lactalbumin, and serum albumins, showcasing its broad applicability in protein engineering. Additionally, the method facilitates the synthesis of antibody-fluorophore conjugates (AFC) and antibody-drug conjugates (ADC), providing promising prospects for therapeutic applications.

The invention provides an autotrophic-heterotrophic synergistic nitrogen and phosphorus removal method. The method comprises the following steps: providing a reactor filled with active hematite; the method comprises the following steps: carrying out biofilm culturing on a mixed bacterial liquid containing a heterotrophic denitrification strain and an autotrophic denitrification strain on a reactor filled with active hematite; introducing a nitrogen and phosphorus-containing water body to be treated into the reactor to realize nitrogen and phosphorus removal; wherein in the nitrogen and phosphorus removal reaction process, the active hematite adsorbs phosphorus in the to-be-treated water body, and meanwhile, the heterotrophic denitrification strains and the to-be-treated water body are promoted to be subjected to a denitrification reaction to realize nitrogen removal; and the cytochrome c generated in the denitrification reaction process is used as an electron donor to promote the nitrogen removal of the autotrophic denitrification strain through the denitrification reaction.

The invention discloses an anti-biological reduction protective agent applicable to phenothiazine photosensitive dyes and application of the anti-biological reduction protective agent in the aspects of phototherapy and immunological enhancement. The protective agent is a biosafe electron acceptor (such as thymoquinone TQ, cytochrome c and ethidium dihydrogen), can effectively inhibit MB from being reduced into colorless LMB in a physiological simulation environment containing NADH / NADPH, accelerates reoxidation of LMB into active MB, and significantly improves the ROS yield (up to 4 times or more). The combined system not only greatly enhances the photodynamic effect of MB in tumor cells and bacteria, but also can strengthen the release of immunogenic death factors (CRT, HMGB1 and ATP), and provides a brand new strategy for developing high-performance phototherapy materials, in-vitro antibacterial systems and immune regulation tools.

The application discloses a method for enhancing the methanogenic efficiency of sludge anaerobic digestion by using an iron-based MOF, wherein conductive materials of 0, 100, 150 and 200 mg / g VS are added to a residual sludge system, and anaerobic digestion is carried out at a temperature of 37±1 DEG C and an initial pH of 7.0 for 30 days. The application first applies MIL-88A(Fe) to a residual sludge anaerobic digestion system, and MIL-88A(Fe) enhances the hydrolysis rate of organic matter and the methane production rate in the anaerobic digestion system. MIL-88A(Fe) mediates the DIET pathway by promoting the secretion of cytochrome C and the production of conductive pili in the anaerobic digestion system, thereby enhancing the electron transport capacity between microorganisms in the system. The application can improve the methane production, alleviate the accumulation and inhibition of volatile fatty acids, promote the acid production rate and the intracellular and extracellular electron transport rate in the anaerobic digestion process, enhance the in-situ conversion of CO2 in the anaerobic digestion system, improve the methane production rate, and provide a new idea for efficient resource utilization of residual sludge produced by a sewage biological treatment process and solid organic waste.

The invention discloses an engineered exosome for inducing pyroptosis of tumor through GSDME as well as a preparation method and application of the engineered exosome. The exosome is constructed by loading a DNA (Deoxyribose Nucleic Acid) methyltransferase inhibitor into the exosome which naturally expresses the GZMB and is derived from immune cells. According to the invention, a methyltransferase inhibitor is used for reversing GSDME methylation and a synergistic effect of GZMB naturally expressed by an NK exosome is used for forming a dual-channel pyroptosis induction mechanism for directly cutting GZMB and indirectly activating caspase-3 cascade to amplify pyroptosis. Besides, the GSDME-N destroys a mitochondrial membrane to release cytochrome C, caspase-3 is continuously activated, positive feedback circulation is formed, and the pyroptosis efficiency is remarkably improved. The engineered exosome can efficiently induce pyroptosis of tumor cells in solid tumors with insufficient immune cell infiltration, remodel an immunosuppression microenvironment and promote CD8T cell infiltration and anti-tumor immune response.

This invention discloses a vitamin C smart delivery nanocomponent based on biomimetic imprinting technology and its anti-tumor application. The core of the nanocomponent is a SiO2 nanoparticle containing cytochrome C, and the surface is directionally imprinted with a vitamin C-specific imprinted cavity using ascorbyl glucoside (AA2G), a stable derivative of vitamin C, as a molecular imprinting template. The preparation steps include: SiO2 nanoparticles containing cytochrome C. 2 The synthesis, amino-functionalization, boric acid-functionalization, template immobilization, directional imprinting, secondary amino-functionalization, template removal, and DMMA modification of NPs-CC led to the construction of the nano-component vMIPDcc containing cytochrome C in its core and having a vitamin C-specific imprinted cavity on its surface. This nanocomponent exhibits high selectivity (IF=8.4) and reliable recognition ability for vitamin C. vMIPDcc achieved an in vivo inhibition rate of 60.2% against colon cancer, providing new ideas and options for the development of anti-colon cancer drugs.

According to the invention, a single-cell Raman technology and a stable isotope labeling combined technology are established, the identification of methane-oxidizing bacteria at the single-cell level and the quantification of the functional activity of the methane-oxidizing bacteria are realized, and the functional microbial cells are sorted. Based on the technology, the invention develops a methane-oxidizing bacteria screening method based on a single-cell Raman spectrum technology and stable isotope labeling, and the methane-oxidizing bacteria are identified and sorted by taking a Raman peak which causes shift of cytochrome c after assimilation as a characteristic peak; and quantifying the functional activity of the methane-oxidizing bacteria according to the deviation degree of the Raman peak. The method has the characteristics of single cell level research, no dependence on culture and non-destructive detection, and is suitable for screening methane-oxidizing bacteria in complex environments such as soil.

The invention is applicable to the technical field of biological medicines, and provides pH responsive tumor targeting nanogel based on dynamic borate bonds as well as a preparation method and application of the pH responsive tumor targeting nanogel. According to the invention, 3-aminophenylboronic acid and 3, 4-dihydroxyphenylacetic acid are used as key groups, in a PBS buffer solution or a Tris-HCl buffer solution with the pH value of 7.5-8.5, dynamic borate bonds are formed through boric acid groups of a polymer ligand 1 and a polymer ligand 2 and benzenediol groups, and the nanogel is constructed through crosslinking and is loaded with cytochrome C. The nanogel can specifically target CD44 high-expression tumor cells, drug accumulation in a tumor area is achieved by means of permeability enhancement and retention effect, pH-responsive drug release is achieved under the tumor microenvironment acidic condition, and the nanogel is good in stability and biocompatibility and remarkable in anti-tumor effect and has good application prospects. A new scheme is provided for delivery of protein drugs and application of the nanogel in cancer combined treatment, and development of cancer precise treatment is promoted.

A method of assessing mitochondria bioenergetics includes obtaining a frozen tissue or frozen biofluid sample of an individual and measuring mitochondria bioenergetics of the frozen tissue or frozen biofluid sample using a substrate for the frozen tissue or biofluid sample. The substrate may include at least one of Glucose; Alamethicin, Nicotinamide adenine dinucleotide (H), and Cytochrome C; Succinate and Rotenone; or Ascorbate+TMPD (Tetramethyl phenylenediamine).

The invention relates to the technical field of nano composite materials, in particular to a photocatalytic nano composite material as well as a preparation method and application thereof. The preparation method comprises three steps of molybdenum disulfide nanoparticle preparation, precursor dispersion and in-situ compounding, is simple in process, low in energy consumption and high in production efficiency, and can realize high-efficiency and low-cost large-scale preparation of the photocatalytic nano composite material. The photocatalytic nano composite material is composed of two semiconductor photocatalytic materials with staggered energy bands, so that not only can the absorption range of light be adjusted, but also the effective separation of photo-induced electron-hole pairs can be realized by transferring photo-induced electrons and holes to different semiconductor photocatalytic materials. Photogenerated electrons and holes generated by light excitation enable the composite material to obtain relatively strong oxidation-reduction ability, and the composite material can be used for preparing products for adjusting mitochondrial functions so as to accurately interfere mitochondrial electron transport chains and realize oxidation of NADH and reduction of cytochrome C.

The invention discloses a recombinant adeno-associated virus. The recombinant adeno-associated virus is prepared by the following steps: inserting a coding sequence of UBA52 protein into a pAAV-CMV plasmid; the preparation method comprises the following steps: co-transfecting a recombinant plasmid, a pHelper plasmid and a pAAV-RC9 plasmid to an AAV-293 cell; and culturing the cells, and extracting the recombinant adeno-associated virus. The recombinant adeno-associated virus can improve the motor ability of a cerebral hemorrhage subject; reducing encephaledema, hematoma level and cerebral moisture content in brain tissues of cerebral hemorrhage subjects; the mitochondrial DNA content is increased; the content of cytochrome C in cytoplasm is reduced, and the content of cytochrome C in mitochondria is increased; the content of caspase-3 and caspase-9 in the brain tissue is reduced; the content of ATP in brain tissue is increased; the number of degenerative neurons in brain tissues is reduced, and neuronal degeneration is recovered or slowed down; the apoptosis of brain tissue neurons is retarded. The recombinant adeno-associated virus has an application prospect.

The invention discloses a separation method for improving the yield, purity and activity of mesenchymal stem cell mitochondria and application. Compared with the existing method for separating the mitochondria of the mesenchymal stem cells, the method disclosed by the invention is used for separating and extracting the mesenchymal stem cells, so that the yield and the purity of the mitochondria of the mesenchymal stem cells can be effectively improved, and the number of the mitochondria of the obtained mesenchymal stem cells can be increased by 2.96 times. According to the separation method, the activity of mitochondria of the mesenchymal stem cells can be well maintained, the content of cytochrome C is remarkably increased, and meanwhile the mesenchymal stem cells have better activity for treating autoimmune hepatitis. In addition, the separation method is simple and convenient, the reagent dosage is small, and the method has wide application prospects in the field of mitochondrial transplantation treatment.