37 results about "Cytochrome c" patented technology

The invention discloses a chemoautotrophic bacteria detection method based on a single cell Raman spectrum and 13C stable isotope labeling. 13C stable isotope is added into a bacterial culture solution or an environmental sample for enrichment culture, centrifugation is performed, cells are collected and used for Raman spectrum detection, and according to the deviation of two characteristic spectrum peaks, namely, the cytochrome c peak deviates from 747 cm <-1 >, 1112 cm <-1 >, 131 cm <-1 > and 1584 cm <-1 > to 725 cm <-1 >, 111 cm <-1 >, 1300 cm <-1 > and 1536 cm <-1 > under exciting light with the wavelength of 532 nm; the peak of phenylalanine shifts from 1002cm <-1 > to 965cm <-1 > under exciting light with the wavelength of 532nm, and chemoautotrophic bacteria can be identified. According to the detection method, the limitation of pure culture is overcome, the bottleneck that the metagenome cannot characterize the carbon sequestration activity is solved, and the single-cell and non-destructive detection of the chemoautotrophic bacteria in the natural environment is realized.

The invention provides a biological sewage treatment accelerant as well as a preparation method and application thereof, and belongs to the technical field of sewage treatment. According to the application, the fruit and vegetable fermentation liquor, the inorganic carbon source, the lipase, the protease, the amylase, the cellulase, the B vitamins, the cytochrome c, the coenzyme Q and the ethylene glycol are used as main components to prepare the accelerant, the accelerant is added into a biological sewage treatment reaction tank, and the biological sewage treatment effect is enhanced by increasing the microbial nitration reaction and denitrification reaction rate.

The present invention belongs to the technical field of anti-cancer drugs, and particularly relates to a cerium vanadate targeted nanoparticle, a preparation method thereof and an application. The cerium vanadate targeted nanoparticle provided by the present invention comprises a drug core, a protective shell layer wrapping the drug core; and a targeting material modified on the outer surface of the protective shell; the drug core comprises CeVO<subgt;4< / subgt> nanoparticles. The cerium vanadate targeted nanoparticle provided by the present invention uses CeVO<subgt;4 nanoparticles as a drug carrier. Since the CeVO<subgt;4 nanoparticles have cytochrome c oxidase-like catalytic activity and cooperate with endogenously overexpressed cytochrome c in cancer cells to reduce and consume O<subgt;2, the oxygen concentration in the tumor microenvironment is effectively reduced. The cerium vanadate targeted nanoparticle provided by the present invention enhances the targeting of the nanoparticle to cancer cells, increases the probability of the nanoparticle entering cancer cells, can activate the drug to play a role in a hypoxic environment, and effectively kills tumor cells.

The present invention discloses a dual-channel colorimetric fluorescence composite probe for detecting trypsin, a detection method and an application thereof. The dual-channel colorimetric fluorescence composite probe comprises orange-red carbon quantum dots and cytochrome C with a volume ratio of 1.5:1. The dual-channel colorimetric fluorescence composite probe of the present invention can, on the one hand, conveniently and sensitively detect trypsin in samples including serum and urine samples, which is of great significance for studying the relationship between trypsin and the occurrence and development of diseases; on the other hand, the presence of a trypsin inhibitor can cause changes in the colorimetric fluorescence signal, thereby enabling the preliminary screening of novel trypsin inhibitors, providing a simple and rapid new method for the discovery of novel trypsin inhibitors. This detection method is expected to achieve the rapid diagnosis of trypsin-related diseases and the rapid screening of novel trypsin inhibitors.

The present disclosure is directed to compositions comprising mitochondrial optogenetics-based gene therapies and their methods of use. In some embodiments, a composition described herein comprises an expression vector comprising a first nucleic acid sequence encoding a channelrhodopsin fusion protein and a second nucleic acid sequence encoding a luciferase protein. In some cases, the first nucleic acid sequence and the second nucleic acid sequence are operably linked to an expression control sequence. In some instances, the channelrhodopsin fusion protein comprises a channelrhodopsin protein linked to an inner mitochondrial membrane-mitochondrial localization signal (IMM-MLS). In some implementations, when the expression vector is expressed, the luciferase protein is localized to the cytosol. In some cases, the IMM-MLS comprises a leading sequence from a mitochondrial inner membrane protein selected from ABCB10, ABCB140, Cytochrome C, and renal outer medullary potassium channel (ROMK).

The invention belongs to the field of protein separation and purification materials, and particularly discloses a polyoxometallate composite material and a preparation method and application thereof in protein separation and purification, 2 g of polyoxometallate C8H30N2O21W5Zr is added into 30 mL of deionized water, stirring is performed until the mixture is clear and transparent, 5 mL of a polyethyleneimine PEI solution is slowly dropwise added into the mixture under the stirring condition, stirring is performed under the room temperature condition for 7 h, and the polyoxometallate composite material is obtained. Washing with deionized water for several times, and drying at 60 DEG C for 24 hours to obtain a white solid. Compared with the prior art, the polyoxometallate composite material ZrW5-coated PEI modified by a high polymer material is prepared through electrostatic interaction among molecules and is applied to separation and purification of cytochrome c in pig hearts; results show that ZrW5-coated PEI has selective adsorption performance on cytochrome c under corresponding conditions. The novel method using ZrW5-coated PEI as the adsorbent has been successfully used for selective separation of cytochrome c in pig hearts, and shows great potential in separation and identification of cytochrome c in pig hearts.

The invention belongs to the technical field of enzyme composite materials and biological catalysis, and particularly relates to an immobilized enzyme catalyst based on a copper-based metal organic framework as well as a preparation method and application of the immobilized enzyme catalyst. Cytochrome c (Cyt c), squaric acid and copper ions are uniformly mixed through an in-situ co-precipitation method, in-situ embedding of Cyt c is achieved through hydrogen bonds and coordinate bonds generated in the Cu-MOF forming process, the Cyt c-coated Cu-MOF compound enzyme catalyst is prepared, a constructed Cu-MOF carrier is of a mesoporous structure, a stable protective microenvironment can be provided for Cyt c, enzyme leaching is effectively inhibited, and the catalyst has the advantages of being capable of being used for preparing the complex enzyme catalyst. The operation stability and the environmental tolerance are remarkably improved, and the catalyst shows efficient catalytic degradability in organic pollutants including phenolic compounds and dyes, and has wide application prospects in the fields of biological catalysis and the like. Moreover, the method is mild in condition, simple and convenient to operate, low in cost and easy for large-scale production.

The invention belongs to the technical field of biomaterial chemistry, and particularly discloses a preparation method and application of functional escherichia coli for expressing laccase. The method comprises the following steps: firstly, according to a full-length cDNA (complementary deoxyribonucleic acid) sequence of a laccase gene, integrating a cotA target gene into a vector plasmid and constructing a recombinant expression plasmid; and introducing the recombinant expression plasmid into escherichia coli to prepare functionalized recombinant escherichia coli. Wherein the active center of the laccase contains copper ions [Cu (II)]. The binding domain of the laccase is mainly composed of histidine, cysteine, methionine and the like, and the laccase can utilize molecular oxygen as an electron acceptor to oxidize various phenolic and non-phenolic compounds and increase active sites except cytochrome c in escherichia coli. Meanwhile, when the substrate is oxidized, the method has the advantages of substrate specificity, mild reaction conditions, harmlessness to the environment and the like, molecular oxygen in air is used as an electron acceptor, only water is generated as a byproduct, the principle of environmental protection is met, and the oxygen reduction capacity of the microbial fuel cell is accelerated.

This invention discloses a method and its application for isolating mitochondria from mesenchymal stem cells (MSCs) to improve their yield, purity, and activity. Compared to existing methods for isolating MSCs, the method of this invention effectively improves the yield and purity of MSCs, increasing the number of mitochondria by 2.96 times. The method also better maintains the activity of MSC mitochondria, significantly increasing cytochrome C content and exhibiting superior activity in treating autoimmune hepatitis. Furthermore, the method is simple, requires minimal reagents, and has broad application prospects in the field of mitochondrial transplantation therapy.

The invention discloses a sensor for detecting cytochrome c and caspase in a single cell as well as a preparation method and application of the sensor. The sensor comprises a theta-type nanotube with two channels, wherein a cytochrome c aptamer and a caspase recognition peptide are respectively fixed on the two channels; one end of the theta-shaped nanotube is a tip, is provided with a theta-shaped nanopore and is used for puncturing into a single cell for sampling. Different detection molecules are respectively fixed in two channels of the theta-type nanotube, and synchronous, high-sensitivity and high-selectivity detection of two key apoptosis markers in a single cell is realized by utilizing the change of charge density on the inner wall of the channel when a target exists. The sensor has good reversibility, stability and reproducibility, can be used for monitoring dynamic changes of cyt c release and caspase-3 activation in a cell apoptosis process in real time, reveals spatial-temporal heterogeneity of an apoptosis mechanism, and provides a powerful tool for cell apoptosis research, disease diagnosis and treatment evaluation.

The invention discloses a high-performance tissue sealant as well as a preparation method and application thereof, and relates to the technical field of medical materials. The tissue sealant is prepared from the following raw materials: amino-containing protein, a multi-arm polyethylene glycol derivative and an optional multi-amino polymer, wherein the amino-containing protein comprises at least one of lactoferrin, phycocyanin, whey protein, beta-lactoglobulin, cytochrome C, recombinant collagen, recombinant fibronectin and alpha-lactalbumin. The sealant provided by the invention has the characteristics of rapid gelling, strong adhesion, high mechanical strength, low swelling, antibacterial property and the like, can realize efficient tissue wound sealing, hemostasis and repair in a wet complex environment, is simple and convenient to prepare, safe in material source and good in biocompatibility, is suitable for being used in various wound scenes, and has wide application prospects. Good application prospects are realized.

The invention relates to a method for noninvasively evaluating age-dependent metabolic characteristics of mouse oocytes. The method comprises the following steps: S1, collecting oocytes of mice at different month ages; s2, treating the oocytes to obtain a sample capable of being used for spontaneous Raman spectroscopy and imaging; s3, performing spontaneous Raman spectroscopy and imaging of different oocytes; s4, processing and analyzing Raman spectrum data; s5, processing and analyzing two-dimensional and three-dimensional Raman imaging; s6, analyzing morphological and spectral characteristics of oocytes of mice of different age groups; and S7: determining the specific age-related change of the lipid content and distribution, the cytochrome c level and the metabolic profile in the mouse oocytes. Compared with the prior art, the method has the advantages that the metabolic change of the oocytes of mice with different maternal ages is analyzed by Raman spectrum for the first time, the age-dependent distribution change of lipid and cytochrome c is revealed through high-resolution subcellular mapping, and a new research scheme is provided for the biochemical mechanism of oocyte senescence.

The invention relates to the technical field of microbial electrosynthesis, and aims to provide a method for promoting microbial electrochemical reduction of CO2 to produce methane by using a high-entropy alloy. Comprising the steps that after pure metal powder of Fe, Co, Cr, Ni, Mn and Mo is treated in a ball milling and mixing mode, high-entropy alloy spherical particles are obtained through sintering and spray granulation; an H-shaped double-chamber electrolytic cell is built, an isometric mixture of a methanogen culture solution and methanogen activated sludge is used as a catholyte, a high-entropy alloy is added, and the methanogen culture solution is used as an anolyte; n2 / CO2 mixed gas is introduced into the cathode chamber, voltage is applied to the cathode chamber, methanogen electrosynthesis reaction and hydrogen evolution reaction are carried out in the cathode chamber, and methane and H2 are generated; and the anode chamber is subjected to oxygen evolution reaction to generate oxygen. The high-entropy alloy FeCoCrNiMnMo is used as a substitute of the cytochrome c, so that the stability, electron shuttling efficiency and metabolic activity of the biological membrane are enhanced; while the methane biosynthesis amount is increased, the energy conversion efficiency of the system is improved, and the reaction energy consumption is reduced.

The invention relates to application of beauveria bassiana in preparation of a medicine for treating multiple myeloma. The invention shows that the Beauverin has a remarkable effect of resisting multiple myeloma, and the action mechanism of the Beauverin is that the expression of anti-apoptotic protein Bcl-2 is inhibited, the expression of pro-apoptotic protein Bax is promoted, the release of cytochrome C is promoted, the activation of Caspase proteases such as Caspase 3 and the like is caused, and finally the apoptosis of cells is caused. Beauveria bassiana regulates an apoptosis pathway through multiple targets, plays a role in resisting multiple myeloma, and provides an experimental basis for developing a novel treatment strategy. Compared with the prior art, the specific action mechanism of the beauveria bassiana is defined, and the value of the beauveria bassiana as a potential anti-tumor drug is highlighted.

The invention discloses a multi-channel nanoprobe, a preparation method thereof and application of the multi-channel nanoprobe in detection of liver ischemia reperfusion injury biomarkers, and belongs to the technical field of in-situ detection and nanoprobe preparation. The nanoprobe is a functionalized metal organic framework nanomaterial which is loaded with gold nanoparticles and a fluorescent dye in sequence and is coated with a hyaluronic acid film; the metal organic framework is prepared by taking 2-methylimidazole and zinc nitrate as raw materials; raman active molecule cytochrome C is modified by the gold nanoparticles through an amidation reaction. The preparation process is simple, the cost is low, the operation is easy, the prepared multi-channel nanoprobe has high sensitivity and excellent biocompatibility, fluorescence and SERS dual-channel simultaneous detection of ATP and O2 <.-> biomarkers in the liver cell injury process can be achieved, and the application prospect is wide. The method can be used for clinically, accurately, efficiently and quickly diagnosing the liver ischemia reperfusion injury and monitoring the disease course in real time.

The invention belongs to the technical field of biomaterial chemistry, and particularly discloses a preparation method of functional escherichia coli coated ascorbic acid for expressing laccase. The method comprises the following steps: firstly, according to a full-length cDNA sequence of a laccase gene, integrating a cotA target gene into a vector and constructing a recombinant expression plasmid; and introducing the recombinant expression plasmid into escherichia coli to prepare functionalized escherichia coli. The functional escherichia coli is coated with ascorbic acid to obtain the product. The laccase can utilize molecular oxygen as an electron acceptor to oxidize various phenolic and non-phenolic compounds and increase active sites except cytochrome c in escherichia coli. Meanwhile, the method has the advantages of substrate specificity, mild reaction conditions, harmlessness to the environment and the like when the substrate is oxidized, molecular oxygen in air is used as an electron acceptor, only water is generated as a byproduct, the method conforms to the principle of environmental protection, and the oxygen reduction capacity of the microbial fuel cell is accelerated.

The invention relates to a combined drug delivery system based on a T cell adapter and adoptive T cells as well as a preparation method and application of the combined drug delivery system, and belongs to the technical field of biological medicines. The invention provides a combined drug delivery system consisting of a T cell adapter D-C-aPDL1 and an azide metabolism labeled adoptive T cell T-N3. The combined drug delivery system is prepared by the following steps: modifying cytochrome C by using DBCO to obtain DBCO-CC, connecting the DBCO-CC and aPD-L1 by using a BS3 cross-linking agent to obtain D-C-aPDL1, and then carrying out metabolism labeling on the adoptive T cell by using azide sugar to obtain T-N3 of which the surface expresses an azide group. Through intravenous injection administration, the T cell adapter is efficiently combined with T-N3 in a targeted manner in vivo, adoptive T cells and tumor cells are coupled in a tumor, and the killing effect of the adoptive T cells on the tumor cells is improved; or the adoptive T cells and the dendritic cells are coupled, activation of the dendritic cells on the adoptive T cells is improved, the anti-tumor curative effect is synergistically improved, and the problem that in the prior art, the adoptive T cell therapy is limited in solid tumor curative effect is solved.

The application discloses a new use of cytochrome C in preparing an ELISA kit and relates to the field of biotechnology. The application uses cytochrome C (Cytochrome c, CC) and streptavidin (Streptavidin, SA) to construct a fusion protein, and the fusion protein has a good detection effect in an ELISA experiment. The fusion protein can simplify a production process, reduce production cost and has a good market conversion prospect.

The application discloses a kind of electroactive microorganism detection method and kit.The application utilizes the catalytic action of electroactive microorganism extracellular electron transfer key protein (outer membrane cytochrome c) and chemiluminescence technology, combined with the photographing function of smart phone, picture information processing technology, provide a kind of electroactive microorganism detection method and detection kit.No need to use any nanomaterial or special equipment, by commonly used smart phone and computer application program can realize signal acquisition, processing, calculation and result output;Convenient and fast, good stability, low cost, simple operation, the whole detection process can be completed without professional training;And response speed is fast, the whole detection process can be completed in 2min;It can be widely used in field detection.

The present application relates to the technical field of nanocomposite, and particularly relates to a photocatalytic nanocomposite, a preparation method and application thereof. The preparation method comprises three steps of molybdenum disulfide nanoparticle preparation, precursor dispersion and in-situ compounding, and has the advantages of simple process, low energy consumption and high production efficiency, and can realize efficient and low-cost large-scale preparation of the photocatalytic nanocomposite. The photocatalytic nanocomposite is composed of two kinds of semiconductor photocatalytic materials with staggered energy bands, can not only adjust the light absorption range, but also realize effective separation of photo-generated electron-hole pairs by transferring photo-generated electrons and holes to different semiconductor photocatalytic materials. The photo-generated electrons and holes generated by light excitation can make the composite material have strong oxidation-reduction capacity, and can be used for preparing products for adjusting mitochondrial function, so as to accurately interfere with the mitochondrial electron transport chain and realize oxidation of NADH and reduction of cytochrome C.

The invention discloses a novel application of cytochrome C in preparation of an ELISA kit, and relates to the technical field of biology. According to the present invention, the cytochrome C (Cytochrome c, CC) and the streptavidin (SA) are adopted to construct the fusion protein, and the good detection effect is provided in the ELISA experiment. By utilizing the fusion protein, the production process can be simplified, the production cost can be reduced, and the fusion protein has a very good market transformation prospect.

The present invention discloses a method for precision engineering of protein bioconjugates by harnessing complex multi-equilibria systems. The method allows for exclusive control over reaction pathways, achieving highly selective outcomes, even with complex substrates such as proteins. By manipulating proteinogenic functionalities, the invention enables pathway selectivity, chemoselectivity, and site selectivity, leading to single-site modifications in proteins. The versatility of the present invention is demonstrated through diverse protein examples, including insulin, RNase A, ubiquitin, cytochrome C, myoglobin, α-lactalbumin, and serum albumins, showcasing its broad applicability in protein engineering. Additionally, the method facilitates the synthesis of antibody-fluorophore conjugates (AFC) and antibody-drug conjugates (ADC), providing promising prospects for therapeutic applications.

The invention provides an autotrophic-heterotrophic synergistic nitrogen and phosphorus removal method. The method comprises the following steps: providing a reactor filled with active hematite; the method comprises the following steps: carrying out biofilm culturing on a mixed bacterial liquid containing a heterotrophic denitrification strain and an autotrophic denitrification strain on a reactor filled with active hematite; introducing a nitrogen and phosphorus-containing water body to be treated into the reactor to realize nitrogen and phosphorus removal; wherein in the nitrogen and phosphorus removal reaction process, the active hematite adsorbs phosphorus in the to-be-treated water body, and meanwhile, the heterotrophic denitrification strains and the to-be-treated water body are promoted to be subjected to a denitrification reaction to realize nitrogen removal; and the cytochrome c generated in the denitrification reaction process is used as an electron donor to promote the nitrogen removal of the autotrophic denitrification strain through the denitrification reaction.

The invention discloses an anti-biological reduction protective agent applicable to phenothiazine photosensitive dyes and application of the anti-biological reduction protective agent in the aspects of phototherapy and immunological enhancement. The protective agent is a biosafe electron acceptor (such as thymoquinone TQ, cytochrome c and ethidium dihydrogen), can effectively inhibit MB from being reduced into colorless LMB in a physiological simulation environment containing NADH / NADPH, accelerates reoxidation of LMB into active MB, and significantly improves the ROS yield (up to 4 times or more). The combined system not only greatly enhances the photodynamic effect of MB in tumor cells and bacteria, but also can strengthen the release of immunogenic death factors (CRT, HMGB1 and ATP), and provides a brand new strategy for developing high-performance phototherapy materials, in-vitro antibacterial systems and immune regulation tools.

The application discloses a method for enhancing the methanogenic efficiency of sludge anaerobic digestion by using an iron-based MOF, wherein conductive materials of 0, 100, 150 and 200 mg / g VS are added to a residual sludge system, and anaerobic digestion is carried out at a temperature of 37±1 DEG C and an initial pH of 7.0 for 30 days. The application first applies MIL-88A(Fe) to a residual sludge anaerobic digestion system, and MIL-88A(Fe) enhances the hydrolysis rate of organic matter and the methane production rate in the anaerobic digestion system. MIL-88A(Fe) mediates the DIET pathway by promoting the secretion of cytochrome C and the production of conductive pili in the anaerobic digestion system, thereby enhancing the electron transport capacity between microorganisms in the system. The application can improve the methane production, alleviate the accumulation and inhibition of volatile fatty acids, promote the acid production rate and the intracellular and extracellular electron transport rate in the anaerobic digestion process, enhance the in-situ conversion of CO2 in the anaerobic digestion system, improve the methane production rate, and provide a new idea for efficient resource utilization of residual sludge produced by a sewage biological treatment process and solid organic waste.

A protein product capable of immobilizing a target and being cleared as required includes a protein (lysozyme, bovine serum albumin (BSA), whey albumin, insulin, α-lactalbumin, fibrinogen, ribonuclease A, cytochrome c, α-amylase, horseradish peroxidase (HRP), pepsin, myoglobin, collagen, keratin, or the like), a protein modifier (a strong oxidizing agent or a strong reducing agent), and a matter to be immobilized (a coating, a pesticide, a chemical fertilizer, a cosmetic, a medical drug, or the like). A protein film can be generated through the phase transition of the protein to immobilize the matter to be immobilized on a target surface, thereby improving the utilization rate of the matter to be immobilized and reducing the resource waste and environmental pollution caused by the loss of the matter to be immobilized. In addition, after the protein film is decomposed, the matter to be immobilized will also be effectively cleared.

The invention discloses an engineered exosome for inducing pyroptosis of tumor through GSDME as well as a preparation method and application of the engineered exosome. The exosome is constructed by loading a DNA (Deoxyribose Nucleic Acid) methyltransferase inhibitor into the exosome which naturally expresses the GZMB and is derived from immune cells. According to the invention, a methyltransferase inhibitor is used for reversing GSDME methylation and a synergistic effect of GZMB naturally expressed by an NK exosome is used for forming a dual-channel pyroptosis induction mechanism for directly cutting GZMB and indirectly activating caspase-3 cascade to amplify pyroptosis. Besides, the GSDME-N destroys a mitochondrial membrane to release cytochrome C, caspase-3 is continuously activated, positive feedback circulation is formed, and the pyroptosis efficiency is remarkably improved. The engineered exosome can efficiently induce pyroptosis of tumor cells in solid tumors with insufficient immune cell infiltration, remodel an immunosuppression microenvironment and promote CD8T cell infiltration and anti-tumor immune response.

This invention discloses a vitamin C smart delivery nanocomponent based on biomimetic imprinting technology and its anti-tumor application. The core of the nanocomponent is a SiO2 nanoparticle containing cytochrome C, and the surface is directionally imprinted with a vitamin C-specific imprinted cavity using ascorbyl glucoside (AA2G), a stable derivative of vitamin C, as a molecular imprinting template. The preparation steps include: SiO2 nanoparticles containing cytochrome C. 2 The synthesis, amino-functionalization, boric acid-functionalization, template immobilization, directional imprinting, secondary amino-functionalization, template removal, and DMMA modification of NPs-CC led to the construction of the nano-component vMIPDcc containing cytochrome C in its core and having a vitamin C-specific imprinted cavity on its surface. This nanocomponent exhibits high selectivity (IF=8.4) and reliable recognition ability for vitamin C. vMIPDcc achieved an in vivo inhibition rate of 60.2% against colon cancer, providing new ideas and options for the development of anti-colon cancer drugs.

According to the invention, a single-cell Raman technology and a stable isotope labeling combined technology are established, the identification of methane-oxidizing bacteria at the single-cell level and the quantification of the functional activity of the methane-oxidizing bacteria are realized, and the functional microbial cells are sorted. Based on the technology, the invention develops a methane-oxidizing bacteria screening method based on a single-cell Raman spectrum technology and stable isotope labeling, and the methane-oxidizing bacteria are identified and sorted by taking a Raman peak which causes shift of cytochrome c after assimilation as a characteristic peak; and quantifying the functional activity of the methane-oxidizing bacteria according to the deviation degree of the Raman peak. The method has the characteristics of single cell level research, no dependence on culture and non-destructive detection, and is suitable for screening methane-oxidizing bacteria in complex environments such as soil.