35 results about "Redox enzymes" patented technology

A method of preparing glucaric acid comprising contacting D-glucose and oxygen with a first catalyst composition comprising a first copper radical oxidase, a single electron oxidizer, and a small molecule activator under conditions suitable for formation of glucodialdose; and contacting glucodialdose and oxygen with a second catalyst composition comprising a second copper radical oxidase, a single electron oxidizer and a small molecule activator under conditions suitable for the formation of a product mixture comprising glucaric acid or salts thereof. A method comprising contacting a sugar with a catalyst composition comprising an oxidoreductase, a single electron oxidizer and a small molecule activator under conditions suitable for the formation of one or more oxidized sugar oxidation products comprising glucaric acid wherein the oxidoreductase comprises at least two copper radical oxidases, at least two mutated copper radical oxidases or combinations thereof.

The present disclosure relates to a genetically modified microorganism satisfying some of predetermined conditions. The predetermined conditions include: (I) succinate dehydrogenase activity or fumarate reductase activity being reduced or inactivated relative to a wild-type microorganism; (II) lactate dehydrogenase activity being reduced or inactivated relative to the wild-type microorganism; (III) the genetically modified microorganism having modified phosphoenolpyruvate carboxylase activity showing resistance to feedback inhibition by aspartic acid in wild-type phosphoenolpyruvate carboxylase activity, or exogenous phosphoenolpyruvate carboxylase activity having higher resistance to feedback inhibition by aspartic acid than that of the wild-type phosphoenolpyruvate carboxylase activity shown by the wild-type microorganism; and (IV) pyruvate:quinone oxidoreductase being reduced or inactivated relative to the wild-type microorganism.

The present invention relates to a biocatalytic synthesis of esters that can also be implemented on an industrial scale. In particular, the invention relates to a process for the production of esters, via a hemiacetal intermediate, consisting of the following steps: – a step of bringing into contact, in a reaction medium, • a mixture of a C1– Cn alcohol and a C1– Cn aldehyde with an enzymatic catalytic system comprising an oxidized cofactor selected from NAD(P)+ and NAD+ and a polypeptide of the oxidoreductase group having preferential dehydrogenase activity toward the formed hemiacetal and the starting aldehyde, or alternatively • a C1– Cn alcohol with an enzymatic catalytic system comprising an oxidized cofactor selected from NAD(P)+ and NAD+ and a polypeptide of the oxidoreductase group having preferential dehydrogenase activity toward the formed hemiacetal, the starting alcohol, and the formed aldehyde, or alternatively • a C1– Cn aldehyde with an enzymatic catalytic system comprising a reduced cofactor selected from NAD(P)H or NADH and a polypeptide of the oxidoreductase group having preferential dehydrogenase activity toward the starting aldehyde and the formed hemiacetal, where n may be any number, and – a step of isolating the corresponding ester.

The present invention relates to the field of biochemical sensors, and discloses a biochemical sensor based on an epitaxial gate type field effect transistor and its application. The biochemical sensor of the present invention includes a substrate, a field effect transistor, a sensitive electrode, and a reference electrode. Among them, the sensitive electrode is electrically connected to the gate of the field effect transistor in the form of an epitaxial gate. The sensitive electrode includes a conductive substrate, and an ordered mesoporous carbon layer, a hydrogen peroxide catalyst, and an oxidoreductase sequentially modified on the surface of the conductive substrate. The sensitive electrode of the present invention exists in the form of an epitaxial gate, which is convenient for replacement, and appropriate oxidoreductases can be selected for different analytes to be detected, so that the biochemical sensor can achieve high-sensitivity detection of a variety of analytes with high redox potentials; since only the sensitive electrode as the epitaxial gate and the reference electrode contact the liquid to be detected during detection, and the field effect transistor does not contact the liquid to be detected, the influence of the solution on the performance of the field effect transistor can be reduced and the device stability can be improved.

The present invention relates to an Fe—S fusion protein acting as an electron transport chain, a novel carbon monoxide:formate oxidoreductase (CFOR) including the Fe—S fusion protein, novel Thermococcus strain BCF12 transformed with CFOR, and the use thereof. According to the present invention, two different enzymes may be physically linked directly to each other through the Fe—S fusion protein of the present invention, and thus electrons generated from any one enzyme may be transported directly to another enzyme through the Fe—S cluster of the Fe—S fusion protein. Accordingly, a reaction that produces a target substance with high efficiency by directly supplying electrons necessary for the production of the target substance is possible without leakage of electrons generated in any one enzyme. In addition, the present invention has an advantage in that the overall enzyme reaction rate and yield can be dramatically improved using a new electron transport reaction. Furthermore, it is possible to ensure the stability of each enzyme by allowing the enzymes to exist in a physically fixed state in cells.

The invention discloses glucose-methanol-choline oxidoreductase and application thereof, the amino acid sequence of the glucose-methanol-choline oxidoreductase is as shown in SEQ ID NO: 1, and the glucose-methanol-choline oxidoreductase is used for enhancing the activity of lignin degrading enzyme so as to improve the capability of degrading lignocellulose of crop straws.

This invention relates to the field of biopharmaceutical technology, and more particularly to the application of curcuminoid compounds in the preparation of drugs for treating schistosomiasis, and pharmaceutical compositions thereof. The curcuminoid compounds used in this invention contain different substituents on their benzene rings, which can effectively regulate the electrophilic and lipophilic properties of the compounds, making them easier to enter the schistosomiasis cell. They interact with redox enzymes within the schistosomiasis cell, disrupting the redox balance and promoting schistosomiasis death. Therefore, they exhibit highly effective therapeutic activity against schistosomiasis and show great promise for application in the preparation of drugs for treating schistosomiasis.

The present invention provides a method for treating wastewater after textile printing and dyeing, which comprises the following steps: after the printing and dyeing wastewater is pretreated by a grid and flotation, it enters a micro-nano bubble enhanced coagulation reactor, and after a mixing reaction, it is precipitated and separated; the coagulation sedimentation effluent enters an electrocatalytic-ozone coupled oxidation system, and is electrocatalytically oxidized under the action of a titanium-based boron-doped diamond electrode, while ozone is introduced for reaction; the electrocatalytic-ozone oxidation effluent enters a bioenzyme enhanced degradation system, laccase and peroxidase are added to an anaerobic granular sludge reactor, and oxidoreductase and hydrolase are added to a membrane bioreactor; the membrane bioreactor effluent enters a multifunctional enzyme immobilized photocatalytic self-repairing membrane system; the clean water after treatment by the multifunctional enzyme immobilized photocatalytic self-repairing membrane system is returned to the production line as recycled water. The present invention achieves efficient removal of new pollutants in printing and dyeing wastewater and long-term stable operation of the membrane.

Provided herein is use of a design principle for recombinantly engineering polypeptides having redox enzyme activity that have improved catalytic efficiency for a noncanonical cofactor while having reduced catalytic efficiency for a natural cofactor. The disclosure further provides utilizing the foregoing design principle for the construction of orthogonal metabolic systems that are not tied to natural metabolism by an organism. Further provided herein is the production of an orthogonal metabolic system that utilized NMN(H) as a driving force for the in vivo conversion of (m)-Bdo to (SS)-BDo or (RR)-Bdo with high purity.

Treatment with epimerase in vitro forms D-psicose from D-fructose dissolved in aqueous solution, and then D-psicose is converted into the oxidized cofactor NAD(P). + A method for preparing an aqueous solution containing allose by reducing allitol to allitol in vitro with NAD(P)H-dependent oxidoreductase, which is accompanied by the formation of a compound, and then enzymatically oxidizing allitol to allose. (Figure 1)

The present invention provides a biosensor capable of being manufactured at low cost and simply, and capable of measuring the concentration of a substance to be measured, such as glucose, with high reproducibility, and a measurement method using the biosensor. A biosensor includes an insulating substrate, an electrode pair provided on the insulating substrate, and a reagent having a redox enzyme and an electron transfer substance placed on at least a working electrode among the electrode pair. The biosensor is used to measure a substance to be measured in a sample supplied to the electrode pair. The biosensor is characterized in that the working electrode is made of one or more nickel alloys selected from a nickel-ruthenium alloy, a nickel-tungsten alloy, and a nickel-vanadium alloy.

The present application relates to engineering strains, and discloses a nicotinamide coenzyme cycle regeneration engineering strain and application thereof. By reconstructing the NAD(H) metabolic pathway with nicotinamide as a synthetic precursor, the accumulation of endogenous NAD(H) in Escherichia coli is improved from three aspects of enhancing the supply of synthetic precursors, knocking out genes related to degradation pathways and enhancing the energy supply of the synthesis path, and a high-yield nicotinamide coenzyme cycle regeneration strain E.coli-NAD(H) is obtained, and the NAD(H) synthesis titer reaches 34.6 μmol / gDCW. The strain can provide sufficient endogenous coenzyme for the whole-cell catalytic reaction based on oxidoreductase, reduce the cost, and lay a foundation for promoting the industrial application of oxidoreductase.

A catalyst composition comprising an oxidoreductase, a single electron oxidizer and a small molecule activator. A method comprising contacting a sugar with a catalyst composition comprising an oxidoreductase, a single electron oxidizer and a small molecule activator under conditions suitable for the formation of one or more oxidized sugar oxidation products.

One aspect of the present invention relates to an oral hygiene enzyme complex for use in the treatment of dental plaque and plaque bacterial biofilms and for the natural whitening of enamel, the complex comprising a combination of the following compounds: sucrase enzymes, particularly those capable of hydrolyzing the β-2,6 and / or β-2,1 bonds of sucrose; a mixture of glucanohydrolase enzymes, including mutanases having exolytic activity and obtained from fungi, and mutanases having endolytic activity and obtained from bacteria, particularly those capable of hydrolyzing (1→3)-α-D-glucans and (1→6)-α-D-glucans; glycoside hydrolase enzymes, particularly those capable of hydrolyzing the (1→6)-α-D-glycosidic bond in monosaccharide dimers and trimers; oxidoreductases, particularly those capable of producing hydrogen peroxide from glucose and monosaccharide monomers; and peroxidase enzymes, particularly those capable of converting thiocyanate to hypothiocyanate in the presence of hydrogen peroxide.

Provided is a biosensor for detecting glucose in body fluid by using a measuring device, the biosensor comprising: a substrate; a plurality of electrodes positioned on the substrate; and a reagent layer, wherein the reagent layer comprises: an oxidation-reduction enzyme; and an electron transfer medium including a ruthenium compound and neutral red.

The invention belongs to the field of microbial fermentation and metabolites thereof, and particularly relates to a fermentation method for simultaneously producing superoxide dismutase and nano-silver particles and a product composition. According to the invention, the stress tolerance characteristic of deinococcus radiodurans is utilized, and the concentration of Ag < + > is controlled, so that the enzyme activity of oxidation-reduction enzymes including SOD (superoxide dismutase) of the deinococcus radiodurans is improved, and meanwhile, the Ag < + > is reduced into nano-silver particles (DR-Ag NPs). After deinococcus radiodurans is stressed by Ag < + >, the product is rich in oxidoreductase including SOD and nano-silver particles (DR-Ag NPs), the product can be prepared into a functional cosmetic product after being compounded with hyaluronic acid and the like, the functional cosmetic product has super-strong anti-aging, anti-wrinkle, whitening, antibacterial and anti-inflammatory effects and the like, and meanwhile, the use of preservatives is avoided.

A modified redox enzyme includes a dehydrogenated redox enzyme and a modification chain segment. The dehydrogenated redox enzyme includes at least one dehydrogenated thiol group, and the modification chain segment has a structure of Formula (1) and is bonded to the dehydrogenated thiol group. The dehydrogenated redox enzyme includes at least one dehydrogenated amino group, and the modification chain segment has the structure of Formula (2) and is bonded to the dehydrogenated amino group.A1 is a first chain segment having a first π-conjugated system, X is hydrogen, alkyl, carboxyl, amide, or ester group, n1 is an integer from 0 to 20, and m1 is an integer from 0 to 20;A2 is a second chain segment having a second π-conjugated system, n2 is an integer from 0 to 20, and m2 is an integer from 0 to 20.

The invention relates to the technical field of pesticide residue treatment, in particular to a method for degrading pesticide residues in fruits and vegetables through cooperation of ultrasonic waves and natural enzymes. The method for degrading the pesticide residues in the fruits and the vegetables through the cooperation of the ultrasonic waves and the natural enzymes comprises the step that in the presence of pulse ultrasonic waves, the natural oxidoreductase is used for treating the fruit and vegetable products, wherein the pulse ultrasonic waves and the natural oxidoreductase have a synergistic effect, so that the degradation of the pesticide residues is realized; the frequency of the pulse ultrasonic waves is 20-100 kHz, the power density is 0.1-1.0 W / mL, a pulse working mode is adopted, and the duty ratio is 20-80%. According to the method, the pulse ultrasonic waves with specific parameters are cooperated with the natural oxidoreductase, so that hydrophobic pesticides such as pyrethroids and organophosphorus which are generally residual and are difficult to remove in fruits and vegetables can be efficiently degraded within a short time.

The invention belongs to the field of agricultural microbial technology and agricultural product safety, and particularly relates to application of a method for preparing a functional biological flora in a targeted biological flora and host original flora coupling mode in rice cultivation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus and host original flora are subjected to coupled symbiotic culture to form specific functional flora, and the specific functional flora is compounded with nutrient carriers such as humic acid potassium fulvate and amino acid chelated trace elements to prepare the targeted biological bacterium water-soluble fertilizer. The microbial agent effectively reduces the bioavailability of heavy metals such as cadmium, lead and arsenic in soil and migration of the heavy metals to rice; meanwhile, the functional flora secretes hydrolase, oxidoreductase and the like, degrades pesticide residues such as organophosphorus and pyrethroid and decomposes the pesticide residues into non-toxic or low-toxicity substances, the method is specially used for solving the problem that heavy metal and pesticide residues in the rice in the filling stage of the rice exceed the standard, and the quality and edible safety of the rice are guaranteed from the source.

The present invention discloses the application of a redox enzyme as a naringenin alcohol dehydrogenase in the biosynthesis of naringenin chalcone, belonging to the technical field of bioengineering. The enzyme is a redox enzyme YlADH_Bm6 derived from Yarrowia lipolytica CLIB122 (the amino acid sequence thereof is shown in SEQ ID No. 2) and a corresponding modified redox enzyme (the amino acid sequence thereof is shown in SEQ ID No. 3). Using the redox enzyme and the modified redox enzyme, together with their recombinant expression cells, they have a high tolerance to substrate concentration, a high conversion rate and mild reaction conditions; they also have excellent performance in the cell fermentation synthesis of naringenin chalcone, and a high yield of naringenin chalcone is obtained. The method for preparing naringenin chalcone according to the present invention is environmentally friendly and easy to operate. Therefore, the redox enzyme and the modified redox enzyme described in the present invention have good application prospects.

The present disclosure provides a technique that is advantageous from the viewpoint of suppressing deterioration of an enzyme while continuously activating a coenzyme. A reaction device (1a) according to the present disclosure comprises an enzyme reactor (10), an enzyme (15), and an electrochemical reactor (20). The enzyme (15) is disposed inside the enzyme reactor (10). In the reaction device (1a), the enzyme reactor (10) and the electrochemical reactor (20) are configured such that a reaction liquid (R) circulates between the enzyme reactor (10) and the electrochemical reactor (20). The reaction liquid (R) contains a substrate and a coenzyme. The enzyme (15) is, for example, an oxidoreductase. The substrate is oxidized or reduced by the enzyme (15). The coenzyme restores the activity of the enzyme (15) after the enzyme has reacted with the substrate.

Provided is a method for managing the accuracy of an electrochemical sensor which includes a base material, an electrode pair that includes a counter electrode and a working electrode including an oxidoreductase electrode provided on the base material, and an outer layer film that covers the electrode pair and which quantitatively measures a target substance on the basis of an electrical signal generated by an oxidoreductase reaction, said method comprising periodically performing electrochemical impedance measurement between the working electrode and the counter electrode and monitoring a change in electrochemical impedance.

The present invention relates to a process for preparing a core-shell microcapsule comprising the steps of: (a) mixing a hydrophobic phase comprising a hydrophobic material, preferably a perfume oil or a flavor oil, and an aqueous phase comprising a first polyelectrolyte and, optionally, a second polyelectrolyte having an opposite net charge to the first polyelectrolyte, to form an oil-in-water emulsion, (b) adding an enzyme, preferably an oxidoreductase to the mixture of step (a), optionally, adding a or said second polyelectrolyte having an opposite net charge to the first polyelectrolyte, and (c) providing conditions sufficient to induce crosslinking to the mixture of step (b) by the oxidoreductase to form a core-shell microcapsule as well as to the microcapsules obtained by said process their use and consumer products thereof.

The present invention relates to the field of food production, in particular the production of pasta. The invention discloses a method for preparing pasta comprising steps of a) mixing water and a ground plant product to obtain a pasta dough, b) kneading the pasta dough, and c) forming the pasta dough into pasta of a desired shape, wherein steps b), c) and, optionally, step a) are performed in a vacuum, and wherein during step a), at least one enzyme selected from the group comprising an oxidoreductase, a carboxylester hydrolase, a hemicellulase, a glucanase, a cellulase, a transglutaminase, a protease, an amylase or a combination thereof is added to the pasta dough.

The invention discloses formate dehydrogenase as well as a preparation method and application thereof, the amino acid sequence of the formate dehydrogenase is as shown in SEQ ID NO: 1, or the formate dehydrogenase has at least 95% sequence identity with the amino acid sequence as shown in SEQ ID NO: 1, and compared with the amino acid sequence as shown in SEQ ID NO: 1, the formate dehydrogenase contains amino acid residue difference at one or more sites of I127, C150, A203 or T344. The formate dehydrogenase disclosed by the invention can be used as a coenzyme regeneration enzyme to participate in an oxidoreductase catalysis reaction depending on a reduced coenzyme NADH, and relatively high enzyme activity and good thermal stability are kept in the reaction process, so that the regeneration efficiency of the reduced coenzyme NADH is maintained, and the generation amount of a product obtained by oxidoreductase catalysis is increased.

The application discloses a corn NADPH quinone oxidoreductase 1 coding gene ZmNQO1 and application thereof, and belongs to the technical field of genetic engineering. The application first discovers that the corn NADPH quinone oxidoreductase 1 coding gene ZmNQO1 regulates the salt stress tolerance of corn, so that ZmNQO1 as a target, the gene is transformed into the genome of corn by constructing a plant expression vector for overexpression of the gene, can drive the overexpression of the target protein, so that the corn plant can grow better under salt stress, and finally promotes the formation of crop yield under salt stress. The gene and the related application can be used for cultivating new corn salt-tolerant breeding materials, and provide a theoretical basis and key gene resources for further corn stress-resistant breeding.

The invention belongs to the technical field of enzyme immobilization, and particularly relates to an in-situ enzyme immobilization method for improving enzyme activity and application of the in-situ enzyme immobilization method. The method comprises the following steps: mixing oxidoreductase with a metal ion solution to obtain an enzyme-metal ion mixed solution; mixing a nitrogen-containing organic molecule with a ligand to obtain a nitrogen-containing organic molecule-ligand mixed solution; and mixing the enzyme-metal ion mixed solution with the nitrogen-containing organic molecule-ligand mixed solution, and carrying out immobilization to obtain the in-situ immobilized enzyme. The method can significantly improve the catalytic activity of the immobilized enzyme, wherein the activity of the immobilized catalase is 2.3 times higher than that of the free enzyme. The prepared enzyme carrier shows excellent anti-interference capability, can tolerate external disturbance of heat, solvents, protease and the like, and has remarkable in-vitro active oxygen scavenging capability. The invention provides a simple and effective strategy which can be used for improving the enzyme catalysis efficiency and has a wide application prospect in a biological catalysis system.

The invention relates to a method for producing 2, 5-furandicarboxylic acid by the in vitro oxidation of 5-formyl-2-furancarboxylic acid present in an aqueous solution to 2, 5-furandicarboxylic acid by treatment with an NAD (P) + dependent oxidoreductase, in which NAD (P) H generated during the oxidation is oxidized back to NAD (P) + by means of an NAD (P) H oxidase via an enzymatic reaction, the enzyme is then removed.

The present invention relates to a variant of a parent NAD (P) H-dependent oxidoreductase comprising (a) a Rosman fold having a common motif GXnGXmG / A, where n is 1, 2 or 3 and m is 1 or 2, and (b) a glycine residue located at 8 to 14 amino acid residues downstream of said common motif, where the glycine residue is mutated in the variant.

A composition comprising a) a curable resin or prepolymer component having ethylenically unsaturated polymerizable groups, b) an ethylenically unsaturated polymerizable monomer, c) an oxidoreductase and d) at least one of an organic peroxide and hydrogen peroxide, wherein the composition comprises between 0.0 and 20.0% by weight of water, calculated on the total weight of the composition.