72 results about "Sucrase" patented technology

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention discloses a comprehensive prevention and control method for tobacco root knot nematode disease containing a biological agent. The method comprises the steps of crop rotation of tobacco and buckwheat / quinoa, host resistance induction of the microbial agent in the seedling raising period, tobacco planting soil quality improvement in the transplanting period and the like. The agronomic characters of tobacco seedlings are effectively improved, and the physical characters of soil are improved; the chemical properties of soil pH, alkali-hydrolyzable nitrogen, available phosphorus, quick-acting potassium, exchangeable calcium, exchangeable magnesium and the like are improved; the activity of soil urease, sucrase, acid phosphatase, catalase and peroxidase is improved; the content of water-soluble phenols, compound phenols and total phenols in soil is reduced; the disease index of the tobacco root knot nematode disease can be remarkably reduced, the survival rate of tobacco is increased, the application amount of chemical agents is reduced, and the healthy and sustainable development of the tobacco industry is promoted.

The invention discloses a nano-material-based nitrogen-phosphorus-potassium compound fertilizer as well as a preparation method and application thereof, and belongs to the field of plant nutrition and soil science research. According to the invention, the nano biochar prepared through nano grinding after pyrolysis of the rice straw is directly mixed with three chemical components, namely the urea, the ammonium dihydrogen phosphate and the potassium chloride, and the sodium bentonite, and the key points of the effects are triple effects of promoting early growth and rapid ripening, enhancing tillering and advancing heading. After the nano biochar is matched with balanced NPK nutrition (such as NPK-II), the activity level of soil key enzymes, including catalase (CAT), alkaline phosphatase (AKP), urease (UE), sucrase (SC) and the like, is remarkably improved. Besides, the composite particles are constructed through the cooperation of the nano-charcoal and the bentonite, and a low-temperature drying process is adopted, so that moisture absorption and caking can be inhibited, and the storage stability and field application performance of the fertilizer are remarkably improved.

The invention provides chickpea protein peptide as well as a preparation method and application thereof. The preparation method of the chickpea protein peptide provided by the invention comprises the following steps: sequentially carrying out enzymolysis on chickpea protein isolate by using neutral protease, pepsin and pancreatin; after the enzymolysis is finished, performing ultrafiltration on an enzymolysis product by using an ultrafiltration membrane with the interception amount of 3kDa, and collecting components with the molecular weight of less than 3kDa; and finally, carrying out gel chromatography on the component, and collecting the corresponding component to obtain the chickpea protein peptide. The chickpea protein peptide prepared by the method has inhibitory activity on DPP-IV, alpha-glucosidase and alpha-amylase, can effectively inhibit the activity of Caco-2 cell sucrase, maltase and DPP-IV, and has a certain inhibitory effect on glucose transport; therefore, the chickpea protein peptide provided by the invention has good hypoglycemic activity, and a new thought is provided for development of food-borne functional protein peptides.

The invention relates to a method for relieving continuous cropping obstacles of ginseng and application of the method, and belongs to the technical field of agricultural planting. The method comprises the following specific steps: planting wrinkled gianthyssop herb on a field after ginseng planting, crushing the wrinkled gianthyssop herb, returning the crushed wrinkled gianthyssop herb to the field, and improving soil for subsequent ginseng planting. The method can effectively relieve continuous cropping obstacles of ginseng, increase the content of soil organic matter, nitrogen, phosphorus and potassium, adjust the pH of soil, improve the activity of soil catalase, reduce the activity of sucrase and urease, optimize the microbial community structure of soil, reduce the abundance of pathogenic bacteria and reduce the incidence rate of root rot and rust rot of ginseng; the yield and quality of ginseng are remarkably improved, the ginsenoside content is increased, meanwhile, use of chemical fertilizers and pesticides is reduced, a green and safe planting mode is achieved, and remarkable economic benefits and ecological benefits are achieved.

The present application relates to the technical field of biology, and discloses a construction method of a Pseudomonas putida suicide vector and application thereof.The construction method of the Pseudomonas putida suicide vector provided by the present application takes a pK18mobsacB vector as a template to obtain a vector skeleton by amplification, and then takes Pseudomonas putida KT2440 bacterial liquid as a template to obtain a strong promoter Prib, upper and lower homologous arms of a gene x to be knocked out, and connects the upper and lower homologous arms to the vector skeleton to obtain the Pseudomonas putida suicide vector.The strong promoter Prib can increase the expression amount of sacB sucrose in the Pseudomonas putida, and enhance the sucrose lethal effect.The Pseudomonas putida suicide vector obtained can be introduced into the Pseudomonas putida, and then the strain with the target gene to be knocked out deleted can be obtained efficiently and accurately through kanamycin positive screening and sucrose negative screening.

The invention discloses an amylosucrase mutant and application thereof in synthesis of alpha-arbutin, the mutant is obtained by mutation of an amino acid sequence of amylosucrase derived from coprophilobacterium mediterranei, and the amino acid sequence of the mutant is shown as SEQ ID NO.4. The invention further discloses a preparation method of the amylosucrase mutant. Compared with a wild strain, the catalytic activity, the temperature stability and the substrate tolerance of the amylosucrase mutant disclosed by the invention are remarkably improved; the recombinant bacteria constructed by using the recombinant bacteria can be used as a whole-cell catalyst to efficiently catalyze a substrate to synthesize alpha-arbutin in a reaction system containing hydroquinone. Therefore, the amylosucrase mutant disclosed by the invention has a good application prospect.

Provided herein is a method for the enzymatic production of gamma-cyclodextrin from sucrose. Optionally, the method comprises contacting sucrose with one or more enzymes to convert sucrose to amylose, and subsequently contacting the amylose with one or more enzymes to convert the amylose to gamma-cyclodextrin. Optionally, the method produces gamma-cyclodextrin in a high yield compared to alpha-cyclodextrin, beta-cyclodextrin, or both.

The invention provides a sugarcane fermentation product, a food quality guarantee product, a preparation method and application, and particularly belongs to the technical field of food preservation. The preparation method of the sugarcane leavening comprises the following steps: crushing sugarcane, and performing first sterilization to obtain sterile sugarcane pulp; inoculating the compound bacteria into the sterile sugarcane pulp for fermentation; the compound bacteria comprise lactic acid bacteria, bifidobacterium bifidum and propionibacterium; a degerming compound enzyme is supplemented in the fermentation process; the compound enzyme comprises xylanase, sucrase, phytase and aminopeptidase; and after fermentation, carrying out secondary sterilization, filtering, and discarding filter residues to obtain the sugarcane fermentation product. The sugarcane leavening disclosed by the invention is prepared from natural raw materials, has good anti-corrosion and anti-oxidation effects, and can prolong the shelf life of food.

The invention belongs to the technical field of bioengineering, and particularly relates to a mutant of sucrose amylase and application of the mutant in alpha-arbutin synthesis. According to the method, a single-point mutant and a multiple mutant are obtained by carrying out site-specific treatment on an amylosucrase sequence as shown in SEQ ID NO.1. Compared with a wild enzyme, the enzyme activity of the mutants is improved to different extents, the enzyme activity of part of the mutants is gradually improved along with increase or superposition of mutation sites, and the synergistic effect of the mutation sites is shown. In all multiple mutants, the activity of a five-site heavy mutant V5 (A284V / D386P / L330 / V92E / A323R) is improved most remarkably, the enzyme activity of the mutant is improved by 6.5 times compared with that of a wild enzyme, the thermal stability is also obviously improved, and the half-life period at 45 DEG C is 1.37 times that of the wild enzyme. The mutant can be applied to synthesis of alpha-arbutin, and the conversion rate is increased by 15.6%, so that the mutant has a good industrial application prospect.

The invention discloses an amylosucrase mutant and application thereof in synthesis of alpha-arbutin, and belongs to the technical field of genetic engineering. An amylosucrase mutant is obtained through an error-prone PCR technology, the mutant is obtained by mutation on an amylosucrase amino acid sequence derived from coprophilobacterium mediterranei, and the amino acid sequence of the amylosucrase mutant is shown as SEQ ID NO.4. The invention further discloses a preparation method of the amylosucrase mutant. Compared with a wild strain, the amylosucrase mutant disclosed by the invention has higher catalytic activity, temperature stability and substrate tolerance; when the catalyst is added into a hydroquinone-containing reaction system, alpha-arbutin can be efficiently catalyzed and synthesized. Therefore, the amylosucrase mutant disclosed by the invention is suitable for industrial production and has a good application prospect.

The invention discloses a sucrase mutant, an expression strain of the sucrase mutant and application of the sucrase mutant in hydrolysis of carbohydrates in soybean milk, and belongs to the technical field of biology. The sucrase mutant disclosed by the invention is simply recorded as InvDz13-TBT-01, and the amino acid sequence of the sucrase mutant is shown as SEQ ID NO: 2. The sucrase mutant InvDz13-TBT-01 is subjected to heterologous expression in bacillus subtilis, a recombinant strain is obtained, and the specific enzyme activity and the stability of protein produced by fermentation of the recombinant strain are remarkably improved. When sucrose is used as a substrate, the specific enzyme activity of the mutant enzyme is improved by 1.94 times, and when raffinose is used as a substrate, the specific enzyme activity of the mutant enzyme is improved by 1.7 times. Under the condition of 35 DEG C, the stability of the mutant enzyme is improved by 4 times. Compared with the starting enzyme, the thermal stability of the mutant enzyme is greatly improved while the specific enzyme activity is maintained. The sucrase mutant disclosed by the invention has a potential value in the application of hydrolyzing carbohydrates such as raffinose in soybean milk.

The present application relates to a kind of recombinant starch sucrolase and its application in the production of resistant starch, belong to the technical field of enzyme engineering.The present application first prepares a kind of preparation method of recombinant starch sucrolase, starch sucrolase derived from polysaccharide Neisseria (Neisseria polysaccharea) is expressed in escherichia coli heterologously, and can significantly improve the activity of starch sucrolase by signal peptide and strengthen expression.Further, the recombinant starch sucrolase is applied to the preparation of resistant starch, and can significantly improve the production efficiency of resistant starch.

The present invention discloses compositions comprising methylxanthines in combination with disaccharides derived from enzymatic conversion of sucrose. More particularly, the present invention discloses compositions comprising methylxanthines and disaccharides derived from enzymatic conversion of sucrose selected from turanose, leucrose, trehalulose and isomaltulose or in combinations thereof.

One aspect of the invention relates to an oral health enzyme complex for the treatment of plaque and plaque bacterial biofilms and for the natural whitening of tooth enamel, comprising the combination of:-a sucrase enzyme, in particular a beta-2, 6 and / or beta-2, 1 bond capable of hydrolyzing sucrose; -a mixture of glucanohydrolases comprising a mutanase having excision activity and obtained from fungi, and a mutanase having excision activity and obtained from bacteria, in particular capable of hydrolyzing (1-3)-alpha-D-glucan and (1-6)-alpha-D-glucan; -a glycoside hydrolase enzyme, in particular a (1-6)-alpha-D glycosidic bond capable of hydrolyzing monosaccharide dimers and trimers; an oxide reductase, in particular capable of forming hydrogen peroxide from glucose and monosaccharide monomers; -a peroxidase, in particular capable of converting thiocyanates into hypothiocyanates in the presence of hydrogen peroxide.

The invention discloses a complex microbial inoculant, a biological stimulant, a preparation method and application. The composite bacterial agent disclosed by the invention comprises Pristia aryabhattai P28 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) 1.65035), bacillus subtilis S1 (the preservation number is CGMCC 1.65034) and bacillus velezensis C1 (the preservation number is CGMCC 1.65033), and the volume ratio of the P28 to the S1 to the C1 is 3 to 1 to 2. The biological stimulant is prepared from kitchen waste aerobic hydrolysate which is obtained by fermenting organic waste kitchen waste serving as a raw material with a complex microbial inoculant with the addition amount of 5% by volume. After the biological stimulant is applied to soil, the total amount of soil organisms (bacteria, fungi and protozoa) can be effectively increased, the soil aggregate stability is improved, and the soil structure is rapidly improved; the content of total organic carbon, granular organic carbon and soluble organic carbon in soil and the activity of soil enzymes (catalase, sucrase, urease and acid phosphatase) are obviously improved in a short time. The method disclosed by the invention has important significance on resource utilization of organic wastes and agricultural sustainable development.

The invention relates to the technical field of food processing, in particular to zanthoxylum armatum oil extracted based on enzymolysis-high-voltage pulse combined assistance and a preparation method thereof. The preparation method comprises the following steps: enzymolysis treatment: blending zanthoxylum armatum and a compound enzyme solution at 45-55 DEG C, wherein the compound enzyme solution comprises alkaline protease, sucrase and cellulase; high-voltage pulse treatment: the electric field intensity is 1.2-1.5 kV / cm, the pulse width is 15-20 microseconds, and the treatment time is 6-9 minutes; solvent extraction: performing centrifugal separation after edible oil extraction. According to the technical scheme, through the synergistic effect of the enzymolysis method and the high-voltage pulsed electric field technology, heat-sensitive flavor and numb-sensitive substances in the zanthoxylum armatum raw materials are reserved to the maximum extent while efficient extraction is achieved.

The present invention discloses compositions comprising methylxanthines in combination with disaccharides derived from enzymatic conversion of sucrose. More particularly, the present invention discloses compositions comprising methylxanthines and disaccharides derived from enzymatic conversion of sucrose selected from turanose, leucrose, trehalulose and isomaltulose or in combinations thereof.

The application discloses a kind of fast swelling, enzyme catalytic degradation hydrogel and its preparation method and application, in the present application based on by sucrose molecule connection multi-arm hydrophilic polymer crosslinked hydrogel, and the swelling rate is fast, and the swelling rate is high, and in the environment of sucrose enzyme degradation rate is fast, can be widely used for preparing in enzyme response biodegradable hydrogel carrier and promote weight loss product.

The invention relates to the technical field of medical detection, in particular to a sucrase-labeled solid-phase reporter, a CRISPR-POCT (clustered regularly interspaced short palindromic repeats-point-of-care testing) detection composition and application thereof. The invention relates to a sucrase labeled solid phase reporter, which is prepared by the following method: S1, modifying T30 ssDNA (deoxyribonucleic acid) or U30 ssRNA (ribonucleic acid) at the 5'end by using DBCO TEG (dibasic copper oxide), and modifying the T30 ssDNA or U30 ssRNA at the 3 'end by using biotin to obtain 5'-DBCO TEG-U30-biotin-3 'RNA or 5'-DBCO TEG-T30-biotin-3 'DNA; s2, carrying out covalent binding on the sucrase modified by the azide group and 5 '-DBCO TEG-U30-biotin-3' RNA (Ribonucleic Acid) or 5 '-DBCO TEG-T30-biotin-3' DNA (Deoxyribose Nucleic Acid) oligonucleotide; and S3, modifying the surfaces of the microbeads with the sucrase-RNA or sucrase-DNA conjugate obtained in the step S2 to obtain a sucrase-labeled solid-phase reporter (ILR).

The invention discloses an amylosucrase mutant and application thereof in synthesis of alpha-arbutin, and belongs to the technical field of genetic engineering. An amylosucrase mutant is obtained through an error-prone PCR technology, the mutant is obtained by mutation on an amylosucrase amino acid sequence derived from coprophilobacterium mediterranei, and the amino acid sequence of the amylosucrase mutant is shown as SEQ ID NO.4. The invention further discloses a preparation method of the amylosucrase mutant. The amylosucrase mutant can be used for synthesis and preparation of alpha-arbutin, the amylosucrase mutant is used as a biocatalyst to convert substrate hydroquinone to generate alpha-arbutin, and the reaction conversion rate of the reaction is proved to be high. Compared with a wild type enzyme, the amylosucrase mutant constructed by the invention has the advantages that the catalytic activity, the temperature stability and the substrate tolerance are obviously improved, the production cost is reduced, and the industrial application value of the amylosucrase is effectively improved.

The application discloses a new application of Korla fragrant pear high-activity polysaccharide in promoting expression of small intestinal disaccharidase, and belongs to the technical field of functional food and medicine. In the application, Korla fragrant pear pulp is freeze-dried, ground, sieved, extracted with hot water, alcohol precipitated, decolorized and deproteinized, separated through a membrane filtration system, further purified through DEAE-Fast flow anion exchange column chromatography, and finally, Korla fragrant pear high-activity polysaccharide KPP-2-a is obtained. The application innovatively finds that KPP-2-a can effectively promote expression and activity of disaccharidase (lactase, sucrase and maltase) in the brush border of small intestinal mucosal epithelial cells, significantly improves secondary disaccharidase deficiency and related symptoms, excavates a new use of the Korla fragrant pear high-activity polysaccharide KPP-2-a and widens the application field thereof, provides a new means for intervention of secondary disaccharidase deficiency, and has a broad prospect and market value.

The invention belongs to the field of biological medicine, and particularly relates to preparation and application of dextran for relieving ulcerative colitis. The dextran GS128 is formed by directly catalyzing and synthesizing dextransucrase Gtf128 generated by heterologous expression in vitro by taking sucrose as a substrate, and the product is obtained by ethanol precipitation, centrifugation, dissolution and dialysis. The compound shows high anti-digestion performance in the process of simulating gastrointestinal tract digestion, and can relieve the symptoms of ulcerative colitis by inhibiting oxidative stress, adjusting the balance of proinflammatory / anti-inflammatory factors and improving the colon barrier.

Provided herein is a method for the enzymatic production of beta-cyclodextrin from sucrose. Optionally, the method involves contacting sucrose with one or more enzymes to convert sucrose to amylose, and subsequently contacting amylose with one or more enzymes to convert amylose to beta-cyclodextrin. Optionally, the method produces beta-cyclodextrin in a high yield compared to alpha-cyclodextrin, gamma-cyclodextrin, or both.

The present invention discloses a self-assembling enzyme for the directed synthesis of uniform low-molecular-weight dextran, named YG-SpyTag-SpyCatcher-padex. YG represents the dextransucrase gene (GenBank No. DQ345760), padex represents the dextranase gene (GenBank No. KF999646.1), SpyTag and SpyCatcher represent two specific short peptides, and the aspartic acid in SpyTag spontaneously forms an isopeptide bond with the lysine in SpyCatcher. The present invention belongs to the field of biotechnology and relates to enzyme engineering technology. The present invention combines dextran sucrase derived from the genetically engineered Escherichia coli bacterium BL21(DE3)-YG-SpyTag with dextranase derived from the genetically engineered Pichia pastoris bacterium X33-SpyCatcher-padex through the SpyTag-SpyCatcher protein covalent coupling system. The obtained self-assembling enzyme can catalyze the direct conversion of the substrate sucrose into dextran with a molecular weight of 3000-4000Da.

One aspect of the present invention relates to an oral hygiene enzyme complex for use in the treatment of dental plaque and plaque bacterial biofilms and for the natural whitening of enamel, the complex comprising a combination of the following compounds: sucrase enzymes, particularly those capable of hydrolyzing the β-2,6 and / or β-2,1 bonds of sucrose; a mixture of glucanohydrolase enzymes, including mutanases having exolytic activity and obtained from fungi, and mutanases having endolytic activity and obtained from bacteria, particularly those capable of hydrolyzing (1→3)-α-D-glucans and (1→6)-α-D-glucans; glycoside hydrolase enzymes, particularly those capable of hydrolyzing the (1→6)-α-D-glycosidic bond in monosaccharide dimers and trimers; oxidoreductases, particularly those capable of producing hydrogen peroxide from glucose and monosaccharide monomers; and peroxidase enzymes, particularly those capable of converting thiocyanate to hypothiocyanate in the presence of hydrogen peroxide.

The invention relates to the technical field of animal breeding, and discloses a method for adjusting the activity of urease and sucrase in lumbricus intestinal tracts. The invention provides application of microalgae in enhancing the activity of urease and sucrase in the intestinal tract of lumbricus, microalgae cell sap containing scenedesmus, chlorella or a combination of scenedesmus and chlorella is used after being mixed with a basic feed for culturing lumbricus, or is directly applied to a culture medium, so that the growth traits such as weight and length of lumbricus can be remarkably improved, and meanwhile, the growth of the lumbricus is improved. By improving the activities of urease and sucrase in the intestinal tract of the lumbricus, the digestion and metabolism efficiency of the lumbricus on a nitrogen source and a carbon source is effectively improved, the problems of low feed utilization rate and slow growth in breeding are fundamentally solved, the standardization and sustainable development of the lumbricus breeding industry are powerfully promoted, and a guarantee is provided for exerting the ecological and medical values of the lumbricus.

The application discloses a method for relieving corn phytotoxicity caused by triazole fungicide seed dressing, and relates to the technical field of crop plant protection and seed treatment. The application discloses that straw source tobacco water can be used for relieving corn phytotoxicity caused by triazole fungicide seed dressing. The specific application mode is as follows: after the straw source tobacco water is diluted to a specific concentration, the straw source tobacco water is compounded with triazole fungicide to prepare a mixed pesticide; and the mixed pesticide is used for seed dressing treatment of corn seeds. Through test verification, the application proves that the straw source tobacco water can improve the germination ability of corn seeds after triazole fungicide seed dressing, promote the growth of corn seedlings, improve the activities of alpha-amylase, plant sucrose and antioxidant enzymes in the corn seeds, increase the content of reduced glutathione, and reduce the contents of malondialdehyde and superoxide anion, so as to relieve the corn phytotoxicity caused by triazole fungicide.

The invention discloses an amylosucrase mutant and application thereof in synthesis of alpha-arbutin, and belongs to the technical field of genetic engineering. An amylosucrase mutant is obtained through an error-prone PCR technology, the mutant is obtained by mutation on an amylosucrase amino acid sequence derived from coprophilobacterium mediterranei, and the amino acid sequence of the amylosucrase mutant is shown as SEQ ID NO.4. The invention further discloses a preparation method of the amylosucrase mutant. Compared with a wild type strain, the catalytic activity, the temperature stability and the substrate tolerance are remarkably improved; recombinant bacteria constructed by the method can be used as a whole-cell catalyst, and alpha-arbutin is efficiently catalyzed and synthesized by taking hydroquinone as a substrate and sucrose as a glycosyl donor. Therefore, the amylosucrase mutant disclosed by the invention has a good application prospect.

The invention discloses an RNA-carried pesticide nanomotor capable of efficiently killing sogatella furcifera and a preparation method of the RNA-carried pesticide nanomotor, and belongs to the technical field of plant protection. The method comprises the following steps: S1, preparing silicon dioxide nanoparticles with a virus surface imitating branch morphology; s2, carrying out mask-assisted selective functionalization and polyelectrolyte layer-by-layer self-assembly, and asymmetrically loading glucose oxidase and sucrase on the surfaces of the nanoparticles; s3, synthesizing double-stranded RNA (Ribonucleic Acid) of the targeted sogatella furcifera chitin synthase gene; and S4, loading the double-stranded RNA on a product obtained in the step S2. The nano-motor disclosed by the invention can decompose cane sugar in rice juice by utilizing a double-enzyme cascade reaction to generate a chemical gradient, so that autonomous chemotactic movement is realized, a vascular bundle end wall barrier of rice is effectively broken through, and an RNA pesticide is efficiently delivered to target pests, so that efficient and specific killing of sogatella furcifera is realized. The method has the advantages of being simple in synthesis, controllable in size, excellent in movement performance, large in loading capacity, environmentally friendly and the like.