322 results about "Sucrase" patented technology

A process for enzymatic preparation of a highly linear poly(α 1, 3 glucan) from sucrose is disclosed. The glucosyltransferase enzyme (gtfJ) from Streptococcus salivarius is used to convert sucrose to a highly linear poly(α 1, 3 glucan) in high titers. Hydrolyzed poly(α 1, 3 glucan) is used as the primer for the gtfJ enzyme reaction resulting in the formation of highly linear poly(α 1, 3 glucan).

Nucleic acid sequences of truncated or mutated alternansucrases, vectors containing these nucleic acids sequences, host cells transformed with the nucleic acid sequences encoding truncated or mutated alternansucrases are provided. Furthermore, a process to recombinantly alternansucrase with a high level of expression, while retaining the enzymatic activity is described.

The invention discloses a method for determining influence of exogenous dsRNA on toxicity of ladybugs. The method comprises the following specific steps: uniformly mixing the exogenous dsRNA into a sucrose solution, directly feeding the ladybugs for 2-3 days and then feeding with pea aphids; then detecting and analyzing the expression changes of target genes in the ladybugs, and observing the biological changes of the ladybugs so as to evaluate the toxicity of the exogenous dsRNA to the ladybugs, wherein the ladybugs include harmonia axyridis, coccinella septempunctata or henosepilachna pusillanima. By the method, direct influence of the exogenous dsRNA on the toxicity of the harmonia axyridis, the coccinella septempunctata or the henosepilachna pusillanima or other ladybugs can be effectively determined; in addition, the method is simple, feasible and high in effectiveness and sensitivity, and has a great significance and an application prospect in study on functions of related genesas well as environmental risk assessment on related RNAi transgenic crops.

The invention belongs to the technological field of environmentally-friendly water treatment, in particular to an enzymology method for preparing polysaccharide bio-flocculant. In the method, sucrose solution with different concentrations of substrate is prepared according to the requirements of products with different molecular weights; and then 1 percent of calcium chloride solution is added and 30 percent of acetic acid buffer solution is used for regulating the pH to be 5.2 to 5.4; the amount of enzyme added is regulated and the pH is regulated to range from 5.2 to 5.4; simultaneously, reaction conditions of temperature, stirring speed, reaction time and the like are controlled; termination is determined to stop the reaction by measuring kinetic viscosity, and then ethanol, methanol or isopropanol is adopted for precipitation, or a ultrafiltration membrane system is used for separation and purification so that various technological indexes of products with different molecular weights can fully reach the quality standard of international market on a novel polysaccharide bioflocculant. A controllable molecular weight can be synthesized by the method, particularly the polysaccharide bioflocculant with ultra-high molecular weight and also can be used for large scale industrialized production.

The invention relates to a novel method for preparing fructo-oligosaccharide, which adopts a thallus anaerobic fermentation technology to produce the fructo-oligosaccharide with the component contentover 50 percent. The method has simple process, does not need a sterile air preparation system equipped for producing the fructo-oligosaccharide by anaerobic fermentation and relevant electric power appliance, and has less equipment investment and low energy resource consumption, thereby greatly saving energy sources. The novel method is applicable to producing the fructo-oligosaccharide of Aspergillus niger BLB-11, which comprises the following steps: carrying out primary or secondary cultivation to obtain a strain for enlarging the culture; transferring the strain to an anaerobic fermentation tank; under the anaerobic condition, adjusting sucrose concentration to 10-60 percent, adding 1-40 percent of bacteroid cells, setting the pH value to be 4-7, carry out the anaerobic fermentation for 10-36 hours at the reaction temperature of 25-60 DEG C to obtain a reaction solution with the content of the fructo-oligosaccharide over 50 percent, micro-filtering, decolorizing, refining and condensation the reaction solution to obtain a fructo-oligosaccharide liquid product, drying the fructo-oligosaccharide liquid product to to obtain a solid fructo-oligosaccharide product after concentration.

The invention relates to a method for producing alternan sucrase by fermenting Leuconostoccitreum and its application, which belongs to the food biology technical field. According to the invention, an alternan sucrase is used for producing Leuconostoccitreum SK24.002 for three-stage cultivation of slant cultivation, seed cultivation and fermentation cultivation, and the alternan sucrase is obtained by separating and purifying a broth and mycelium cells. The method for producing alternan sucrase by using the Leuconostoccitreum SK24.002 possesses the advantages of short fermentation time and low cost, the safety performance is reliable, and the deep fermentation of liquid and cell cultivation with high density can be carried out, thereby the method of the invention is suitable for large scale production. The activity of the produced alternan sucrase can reach more than 10U/mg. A fermented product is non-toxic, and can be directly used for producing a plurality of novel functional carbohydrates possessing prebiotics efficacy.

The invention aims to provide a sucrose phosphorylase mutant and application thereof in the production of glycerol glucoside. The sucrose phosphorylase mutant which is protein is obtained by replacingthe 341st-site amino acid residue, which is leucine, of sucrose phosphorylase with other amino acid residues. The protein can be BaSP/L341W protein obtained by replacing the 341st-site amino acid residue, which is the leucine, of the sucrose phosphorylase with tryptophan. The sucrose phosphorylase mutant has the advantages that by the primer point mutation of the 341st-site amino acid residue ofthe wild sucrose phosphorylase derived from bifidobacterium adolescentis, the specificity of 2-glycerol glucoside produced by using the sucrose phosphorylase mutant, sucrose and glycerin as the raw materials, and the yield of the byproduct 1-glycerol glucoside is lowered; the sucrose phosphorylase mutant has an important application value in the field of the production of the 2-glycerol glucoside.

The invention relates to a compound feed for a weaned pig. The compound feed is prepared from the following raw materials in parts by weight: 25-40 parts of corn, 15-20 parts of extruded corn, 15-20 parts of bean pulp, 2-5 parts of whey powder, 7-11 part of soybean peptide, 2-6 parts of plasma protein powder, 1-5 parts of soya bean lecithin, 1-5 parts of palm kernel oil, 0.012-0.015 part of 5000-unit phytase, 1-2 parts of mountain flour, 0.4-0.5 part of monocalcium phosphate, 0.05-0.2 part of choline, 0.1-0.5 part of salt, 2-5 parts of saccharose, 1-3 parts of vitamin premix, 1-3 parts of microelement premix, 0.2-0.8 part of lysine, 0.1-0.3 part of methionine, and 0.1-0.3 part of threonine. The compound feed can well meet nutritional requirement of the weaned pig, improves the digestibility of the weaned pig for nutrient substance, improves the immunity of the weaned pig, promotes rapid and healthy growth of the weaned pig, and guarantees the survival rate of the weaned pig.

The invention provides a method for on-line synthesizing saccharose-6-laurate by lipase catalysis, comprising: using saccharose and vinyl laurate, with a mol ratio of 1:8-12, as raw materials, using 0.5-10g of Lipozyme TLIM as a catalyst, and using a mixed solvent of tertiary amyl alcohol and DMSO as a reaction solvent, uniformly filling Lipozyme TLIM in a reaction channel of a microfluidic channel reactor, wherein the internal diameter of the reaction channel of the microfluidic channel reactor is 0.8-2.4mm, and the length of the reaction channel is 0.5-1.0m; continuously introducing the raw materials and the reaction solvent into the reaction channel to perform acylation reaction under 40-55 DEG C for 20-35min, on-line collecting the reaction solution, and then obtaining the saccharose-6-laurate after conventional post-treatment on the reaction solution. The method of the invention has advantages of short reaction time, high selectivity and high yield.

The invention relates to exoinulinase Z2-5 with low-temperature activity and a gene of the exoinulinase Z2-5. An amino acid sequence of the exoinulinase Z2-5 from sphingobacterium sp. is shown as SEQIDNO.1. The invention provides the gene Z2-5 encoded with the exoinulinase, and a recombinant vector and a recombinant strain of the exoinulinase gene Z2-5. The exoinulinase has the properties that the optimum pH value is 7; after the exoinulinase is treated by a 0.1 M buffer solution of which the pH value is 9.0 at room temperature for 1 hour, the activity of the exoinulinase also can be kept at over 30 percent; the optimum temperature is 40 DEG C, the enzyme activity of the exoinulinase is about 40 percent at the temperature of 10 DEG C and 50 DEG C, and the enzyme activity of the exoinulinase is about 10 percent at the temperature of below 0 DEG C; after the exoinulinase is treated at the temperature of 50 DEG C for 1 hour, the enzyme activity of exoinulinase also can be kept at over 30percent; and substrates of cane sugar, fructosan and the like can be hydrolyzed, so the exoinulinase belongs to the reaction selectivity of the hydrolysis of a fructose glycosidic bond. By the exoinulinase Z2-5, synanthrin can be hydrolyzed to prepare high fructose corn syrup, so the exoinulinase Z2-5 is used for food industry.

The invention discloses a co-catalytic reaction system for preparing rare ginsenosides. The co-catalytic reaction system comprises glycosyltransferase GT (a); sucrose synthase SUS (b); uridine diphosphate UDP (c); and saccharose (d). Experiments show that in the presence of the saccharose and little UDP, an enzyme combination composed of the glycosyltransferase and the saccharose is capable of replacing an in-vitro reaction system to synthesize UDP sugar, one of expensive materials for the rare ginsenosides, and efficiently and economically converting substrates such as protopanoxadiol or protopanaxatriol into the rare ginsenosides, wherein the UDP is about 1/4 of the UDP sugar in price and is 1% of its original dosage; thus, preparation cost of the rare ginsenosides is greatly saved, and large-scale commercial preparation of the ginsenosides is better facilitated.

The invention concerns aspergillus niger 9891 CGMCC NOú‘0991, which produces alantin endonuclease, and clones alantin endonuclease gene. The result of target gene fragment sequence analysis indicates that open reading frame of gene not containing signal peptide is 1485bp, and codes 509 amino acid, the said protein molecular weight is 55.9KD. The homolog of said gene with counterpart of aspergillus niger (Ohtak.et alú¼ 1998)and Aspergillus ficuum(Uhmú¼t.ú¼et alú¼1998) are respectively 92úÑ and 95úÑ. The alantin endonuclease gene is inserted into Pichia pastoris expression vector, thus recombinant of transformed Pichia pastoris is obtained. The said gene expresses in Pichia pastoris, and expressed product has its function. The expression amount of good recombinant I3-50 is 84 times higher than alantin endonuclease of initial strain. Recombinant yeast is induced and fermented. The analysis about recombinant enzyme shows that its optimum PH is 5.5ú¼ and optimum reaction temperature is 55íµ..

The invention belongs to the technical field of extraction of natural products and in particular relates to an extraction method for blueberry anthocyanin. The extraction method comprises the following steps: firstly, crushing blueberries into blueberry pulp; then, adding a biological complex enzyme for enzymolysis; carrying out ultrasound-assisted extraction, centrifuging and collecting supernatant, carrying out ultrafiltration concentration and spray drying to obtain the anthocyanin. The biological complex enzyme is a compound of acidic cellulase, acidic sucrase and tannase. The blueberry anthocyanin extracted by adopting biological complex enzyme enzymolysis and ultrasound-assisted extraction manners is high in purity and low in cost, and the operation is simple and convenient; an extraction process is green and environmentally friendly; an anthocyanin product has no residues of toxic chemical reagents and the obtained anthocyanin is safer and more reliable; the extraction efficiency of the anthocyanin reaches 90 percent or more.

The invention relates to B. vallismortis HSB-2 and application thereof. The preservation number of the B. vallismortis HSB-2 is CGMCC No.15305, and the sequence of 16S rDNA is shown as SEQ.ID.NO.1. The invention further relates to the application of the B. vallismortis HSB-2 to preparation of bacterial fertilizer for relieving the successive cropping obstacle of apples. The B. vallismortis HSB-2 has an obvious antagonistic effect on four types of Fusarium fusarium pathogenic bacteria causing the successive cropping obstacle of the apples; the bacterial fertilizer manufactured through the B. vallismortis HSB-2 can promote growing of the overground parts and the underground parts of Malus malus hupehensis seedlings, and at the rhizosphere, colonization can be stabilized, and growing of the four types of Fusarium fusarium pathogenic bacteria of solani, proliferatum, verticillioides and oxysporum, causing the successive cropping obstacle of the apples, in soil can be remarkably restrained;and activity of soil urease, phosphatase, sucrase and root system protective enzymes, namely SOD, POD and CAT can be remarkably improved, and the successive cropping obstacle can be relieved.

The invention provides heat-resisting cryoprotectant for classical swine fever live vaccines and a preparation method and application thereof, and belongs to the technical field of veterinary biological product manufacturing. The cryoprotectant is prepared from, by mass, 0.2-2.5% of synanthrin, 0.5-12% of glucan, 1-6% of polyvinylpyrrolidone, 0.5-12% of lactose, 0.2-6% of saccharose, 0.05-2.5% of polyethylene glycol, 0.005-0.6% of histidine, 0.05-4% of cysteine and 0.2-4% of a DMEM culture medium, and the rest is water for injection. The invention further provides a preparation method and application of the heat-resisting cryoprotectant. The heat-resisting cryoprotectant does not contain foreign protein or gelatin, stress reactions and side effects on animals are avoided, the safety of the vaccines is greatly improved, the antigen activity can be efficiently protected in the freeze-drying and storing process, and the stability of the vaccines is improved.

A long-cycle lipoid of 10-hydroxyamptothecin and its freeze dried medicine for treating cancer are prepared from lecithin, PEG modified bipid, cholesterol, mycose or cane sugar, alcohol and dichloromethane.

The invention discloses a bacterium for degrading benzo(a)pyrene and application thereof. The bacterium is acinetobacter sp.Bap30, CGMCC No.4586. The strain can grow with benzo(a)pyrene as the unique carbon source and energy, undergoes shake culture at the temperature of 37 DEG C for 20 days in the inorganic salt culture medium with benzo(a)pyrene concentration being 40mg/L and has degradation rate toward benzo(a)pyrene being 28.66%. The degradation rate of the strain toward benzo(a)pyrene can be effectively improved by adding defined amount of co-metabolic carbon sources, such as cane sugar and maltose. When another polycyclic aromatic hydrocarbon phenanthrene is taken as a co-metabolic substrate and exists by being mixed with benzo(a)pyrene, the degradation rate of the strain toward benzo(a)pyrene can be improved to 48.87% and meanwhile, the strain can realize complete removal of phenanthrene. The strain provided by the invention can provide new microorganism resources for degradation of polycyclic aromatic hydrocarbons in the water environment or soil environment.

The present invention discloses to an organic and inorganic compound fertilizer special for promoting growth and improving disease resistance of lotus roots. The compound fertilizer is prepared from fermented chicken manure, wheat straw, mushroom residue, plant ash, wormcast, urea, ammonium zinc phosphate, ammonium polyphosphate, potassium nitrate, trace elements, compound bacteria, humic acid, chitosan, alginic acid, 3, 4-dimethylpyrazole phosphate, sodium tetraphenylborate, ammonium citrate, potassium thiosulfate, polyaspartic acid, dicyandiamide, sodium nitrophenolate, yttrium nitrate, nanocarbon, rotenone and sucrase. The compound fertilizer is reasonable in formula, balanced in nutrition, capable of promoting the growth of the lotus roots and improving disease resistance of the lotus roots at the same time, and high in fertilizer utilization rate.

The invention discloses an indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit of an animal rabies neutralizing antibody. Rabies virus totivirus particles purified by using a linear sucrose gradient zonal centrifugation method are coated on an enzyme label plate of the kit. The coating has high antigen purity and is capable of detecting antibodies generated by proteins, such as rabies virus glycoprote, nucleoprotein and the like. Found from researches, the kit disclosed by the invention has specificity of being up to 100%, minimum detectable quantity of sensitivity of 0.0625 IU/ml and a variation coefficient CV (repeatability) of 2.74%. The kit has good stability, simple operation and low cost and is applied to popularization and application.

The invention belongs to the technical field of biosensors, and in particular, relates to a high-throughput nucleic acid aptamer sensor for detecting insulin and a preparation method thereof. Nano gold particles are immobilized and combined on a micro-porous plate, and the nano gold particles and an insulin nucleic acid aptamer are combined on the micro-porous plate by Au-S bonds; at the same time, sucrase and a DNA complementary strand partially complementary with the insulin nucleic acid aptamer are immobilized on the nano gold particles, the insulin nucleic acid aptamer and the DNA complementary strand form a stable double-strand structure according to a base pairing principle, and a DNA complementary strand/sucrase/nano gold detection probe is prepared; the probe is immobilized to a nano gold micro-porous plate of the insulin nucleic acid aptamer, and the high-throughput nucleic acid aptamer sensor for detecting insulin is obtained. The application potential of a nucleic acid aptamer molecular recognition system in the biomedical field becomes reality, and simple and real-time detection can be really realized in accident spots or other occasions, so the high-throughput nucleic acid aptamer sensor has wide application prospects.

An exercise-enhancing food tablet, preferably a hard or chewable candy or mint, is disclosed for enhancing exercise and physical fitness when ingested before, during, and/or after exercise. The edible tablet includes a mixture of carbohydrate and protein, the protein portion being between 20% and 40% of the mixture by weight, and preferably between 25% and 33% of the mixture by weight. The protein portion is preferably whey protein, but can be any combination of whey protein, soy protein, and pea protein. The carbohydrate portion of the mixture is preferably 85% dextrose and 15% fructose, but can be any combination of dextrose, maltodextrin, fructose, and sucrose. In preferred embodiments, sodium, potassium, magnesium, vitamin C, and/or caffeine are also included. The tablets can also include a flavoring, such as spearmint, peppermint, or fruit flavoring. In preferred embodiments the tablets weigh substantially 2 grams, and an adult serving is eight tablets.

The invention provides a microwell plate for quantitatively detecting biotin with a microbiological method, a kit and a preparation method of the microwell plate. By means of addition of a trehalose/saccharose and calcium chloride mixed liquid protective agent during preparing and improvement of a microbial solution drying method, microbial strain loss in the kit is reduced, and accuracy of a biotin detection result is improved. Viable counting proves that the number of active microbes preserved in lactobacillus plantarum dry foam in the microwell plate prepared with the method is 93%-95% of the total number of microbes in the added microbial solution while at least about 20% of microbes are lost with a common freeze-drying method.