125 results about "Oxidoreductase" patented technology

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The invention relates to the technical field of microbial metabolism engineering, in particular to a corynebacterium glutamicum producing strain for efficiently producing D-pantothenic acid as well as a construction method and application of the corynebacterium glutamicum producing strain. The construction method specifically comprises the following steps: (1) by taking wild corynebacterium glutamicum ATCC 13032 as a chassis, weakening ilvA, and constructing DPA1 through mutation expression; (2) knocking out the quinone pyruvate oxidoreductase and inserting a panBCE gene expression cassette into the quinone pyruvate oxidoreductase to construct DPA2; (3) introducing and using a pEC-xk99E overexpression plasmid to screen and express Escherichia coli W3110, Bacillus subtilis 168, and endogenous acetohydroxy acid synthase, acetolactate isomerized reductoisomerase and 2-hydroxy acid dehydratase, and carrying out a step (2) of carrying out a step (3) of screening and expressing the Escherichia coli W3110, Bacillus subtilis 168, and endogenous acetohydroxy acid synthase, acetolactate isomerized reductoisomerase and 2-hydroxy acid dehydratase in the DPA2; and (4) under the control of a strong promoter, carrying out tandem overexpression on the screened ilvBNCD gene by using pEC-xk99E, constructing DPA3, and enhancing a pantothenic acid main synthesis pathway and an upstream pathway pulling force, thereby obtaining the corynebacterium glutamicum for efficiently producing D-pantothenic acid. The strain provided by the invention can produce D-pantothenic acid with high yield and high conversion rate by utilizing a carbon source, and has important industrial application value.

The present disclosure provides recombinant adeno-associated virus (rAAV) vectors, methods of treating ocular conditions, pharmaceutical compositions, and other compositions and methods, wherein the rAAV vectors comprise a polynucleotide encoding an oxidoreductase. Methods of treatment may include administration to the lacrimal gland.

The invention discloses an aspergillus oryzae engineering bacterium as well as a construction method and application of the aspergillus oryzae engineering bacterium, and relates to the technical field of genetic engineering. The method comprises the following steps of: (a) connecting and fusing an open reading frame of an endogenous gene with a fungal laccase copper oxidoreductase structural domain and a target heterologous protein gene through a dual-basic amino acid endopeptidase cleavage site gene; or connecting and fusing an open reading frame of an endogenous gene with a multi-copper oxidase structural domain and a target heterologous protein gene through a dual-basic amino acid endopeptidase cleavage site gene; and (b) at least one of a gene Aovps10, a gene AosedD and a gene Aoemp47 is knocked out. The aspergillus oryzae engineering bacterium provided by the invention can realize high-efficiency expression of the heterologous protein, and the extracellular yield of the heterologous protein is obviously improved.

The invention discloses an oxidoreductase mutant and application thereof, and belongs to the field of enzyme engineering. The invention provides a recombinant oxidoreductase HbKR mutant which is used for catalytically producing (R)-decalactone. The oxidoreductase HbKR mutant disclosed by the invention realizes soluble expression in Escherichia coli BL21 (DE3), and the oxidation activity of the oxidoreductase HbKR mutant is improved by 4.61 times compared with that of a parent enzyme. An oxidoreductase HbKR mutant and lactone hydrolase Rpa3624 derived from Rhodopseudomonas palustris are combined for use, reaction is performed for 0.5 h under the conditions that the temperature is 35 DEG C and the pH value is 10.5, compared with a wild type, the yield of (R)-delta-decalactone is increased to 48.2% from 42.3%, and the stereoselectivity is increased to 98.7% from 94.2%.

The invention provides application of aspergillus aculeatus in fermentation processing of traditional Chinese medicine indigo naturalis. The invention further provides application of the combined use of the pediococcus pentosaceus and the aspergillus aculeatus in fermentation processing of the traditional Chinese medicine indigo naturalis. The combination of the aspergillus aculeatus and the pediococcus pentosaceus has obvious advantages in the fermentation of the indigo naturalis. The invention also provides a preparation method of indigo naturalis. In the aspect of an enzyme system, beta-glucosidase generated by the aspergillus aculeatus and the pediococcus pentosaceus can promote dissolution of an indigo precursor substance indole glycoside. In addition, other specific enzymes, such as glucoside hydrolase or oxidoreductase and the like, can also be generated, complex components in indigo naturalis are further decomposed through mutual cooperation of the two enzymes, more precursor substances are released, generation and accumulation of indigo blue and indirubin are jointly promoted, and the content of indigo blue and indirubin is increased. In addition, growth and metabolism of harmful microorganisms can be inhibited, harmful substances possibly generated in the indigo naturalis fermentation process are reduced, and the quality and safety of indigo naturalis products are improved.

The present invention relates to a variant of a parent NAD(P)H dependent oxidoreductase comprising (a) a Rossman fold with a consensus motif GXnGXmG / A, wherein n is 1, 2 or 3 and m is 1 or 2, and (b) a glycine residue being located 8 to 14 amino acid residues downstream of said consensus motif, wherein in the variant the glycine residue is mutated.

The present disclosure relates to a method of forming electrical signal sensor. The method includes the following operations. An oxidoreductase and an amphiphilic molecule are dissolved into a solvent to form a solution, in which the amphiphilic molecule includes alkyl sulfuric acid, alkyl sulfate, alkyl sulfonic acid, alkyl sulfonate, alkyl ammonium, alkyl ammonium salt, alkyl phosphoric acid, alkyl phosphate, alkyl carboxylic acid, alkyl carboxylate, alkylboronic acid, alkylborate, or combinations thereof. The solution is coated on an electrode layer. The solution is dried.

The application provides a human Helicobacter pylori detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology, amplifies the genomic fragment containing the four-strand DNA sequence in the human Helicobacter pylori genome, and realizes rapid and visual detection of the human Helicobacter pylori by adding hematin and oxidoreductase substrate for color reaction.

The present invention discloses the use of an oxidoreductase and its mutants in the biosynthesis of naringol, belonging to the field of bioengineering technology. The present invention, for the first time, obtains an oxidoreductase with high conversion capacity for naringol from the marine killer yeast (Wickerhamomyces anomalus M15), and obtains an oxidoreductase mutant with even higher capacity through site-directed mutagenesis. Compared with existing oxidoreductases capable of catalyzing the conversion of naringol, the oxidoreductase and its mutants have good substrate tolerance, high conversion rate, and high salt tolerance. The oxidoreductase and its mutants provided by the present invention provide conditions for the in vitro enzymatic synthesis of naringol, achieving an environmentally friendly and efficient catalytic conversion of naringol to the naringol product. The present invention will provide an important tool enzyme for the synthesis of naringol and bring significant economic benefits to the naringol synthesis industry.

The purpose of the present invention is to provide a sensor for measuring a biological component concentration with a means for improving the reaction efficiency.　The present invention provides a sensor for measuring a biological component concentration, the sensor comprising: an electrode that includes at least a working electrode; and a reagent layer that is formed on the electrode. The reagent layer includes an oxidoreductase, an electron transfer body having an electron transfer material bonded to a terminal of branched-chain polyethylene glycol, and a single-walled carbon nanotube.

The invention belongs to the field of microorganism application, and particularly relates to a bacterium capable of degrading pyrethroid pesticides, and a preparation and application thereof. The invention relates to the field of biotechnology, and in particular relates to a pseudomonas aeruginosa, which is named as paracoccus panotrophus BB-2 and is preserved in the China Center for Type Culture Collection on May 26, 2025 with the preservation number of CCTCC NO: M 20251184, and pseudomonas aeruginosa, which is named as pseudomonas aeruginosa BS-3 and is preserved in the China Center for Type Culture Collection on May 26, 2025 with the preservation number of CCTCC NO: M 20251185. The invention further relates to a mixed bacterial agent of the paracoccus pantotrophus BB-2 and the pseudomonas aeruginosa BS-3, it is found that the paracoccus pantotrophus BB-2 and the pseudomonas aeruginosa BS-3 can be subjected to synergistic symbiosis and secrete and produce extracellular hydrolase and multiple oxidoreductases, pyrethroids are efficiently degraded, and the removal efficiency of the multiple pyrethroids reaches 90% or above.

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.

Process for the production of 5-hydroxymethyl-2-furancarboxylic acid (HMFA), wherein 5-hydroxymethylfurfural (HMF), which is present in an aqueous solution, is oxidized to obtain 5-hydroxymethyl-2-furancarboxylic acid (HMFA) by treatment with an NAD(P)+-dependent aldehyde dehydrogenase in vitro with NAD(P)H being formed; after said oxidation step the NAD(P)H formed during the oxidation is enzymatically regenerated with an oxidoreductase back to NAD(P)+, and a keto compound is used as a cosubstrate of the oxidoreductase.

The invention relates to the technical field of microorganisms, and provides a rhizobium mutant and application thereof, the rhizobium mutant is HH103 delta nuo, and is a mutant obtained by taking sinorhizobium fredii (sinorhizobium fredii) HH103 as a starting strain and performing gene knockout on a nuo control unit in an NADH oxidoreductase subunit I of the sinorhizobium fredii HH103. When the HH103 delta nuo is inoculated in the soybean seedling stage, the plant shows that the leaves are darker green, the plant biomass is up-regulated, the nitrogenase activity of a single plant is obviously higher than that of a wild type strain, and the plant height is integrally increased, so that the HH103 delta nuo improves the symbiotic effect of the rhizobium and the soybean.

In one embodiment of the present invention, there is provided a reagent layer comprising a polymer comprising a proton accepting group in a repeating unit and having a pH buffering capability, and an oxidoreductase which oxidizes or dehydrogenates an analyte.

The application provides a porcine parvovirus detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology to amplify the genomic fragment containing the four-strand DNA sequence in the porcine parvovirus genome, and realizes rapid and visual detection of porcine parvovirus by adding hematin and oxidoreductase substrate for color reaction.

To provide compositions for increasing the level of NAD(P)H:quinone oxidoreductase 1 (NQO-1), or increasing its gene expression.SOLUTION: Provided is a composition for increasing the level of NAD(P)H:quinone oxidoreductase 1 (NQO-1) or increasing its gene expression in a subject. The composition comprises a combination of sulforaphane and a glucan as active ingredients, which combine to produce a synergistic effect between the active ingredients. The glucan is provided as a Ganoderma lucidum extract or powder. The Ganoderma lucidum extract preferably contains about 1 to about 75 wt.% of one or more glucans. Furthermore, the one or more glucans are preferably β-glucan and / or 1,3-β-glucan and / or 1,6-β-glucan.SELECTED DRAWING: Figure 1

This invention discloses a Bio-Fenton-driven in-situ polyacrylamide injection fluid depolymerization composition, its preparation method, and its application. The composition comprises an oxidoreductase, reducing sugar, and a transition metal ion source. In use, the composition is added to the injection fluid. The oxidoreductase catalyzes the in-situ oxidation of the reducing sugar to generate hydrogen peroxide, and the transition metal ions from the transition metal ion source mediate a Bio-Fenton reaction, generating highly reactive hydroxyl radicals that attack the polyacrylamide backbone, causing it to break and depolymerize. The final depolymerization viscosity can be reduced to below 5 mPa·s. The composition of this invention works effectively within the temperature range of 45–90°C and pH range of 4.0–10.0. The depolymerization process is stable and controllable. All components and degradation products are non-toxic and biodegradable. All components are in solid form, making storage and transportation safe and convenient.

An immobilized enzyme fiber contactor includes a plurality of fibers disposed within a hollow conduit. The fibers have attached thereto an enzyme selected from the group consisting of an oxidoreductase, a transferase, a hydrolase, a lyase, an isomerase or a ligase. The enzyme may be attached to the fiber via an anchor group and optionally a bifunctional crosslinking agent. The enzyme may be applied, stripped, and re-applied without detaching the reactor or discarding the fibers. The immobilized enzyme fiber contactor can be used for carrying out a two-phase or single-phase enzymatic reaction.

A novel quantitative method, an enzyme for quantitative determination, a quantitative kit, and a quantitative sensor for quantitative determination of the concentration of EAP, which is a biomarker of depression, are provided. A quantitative method for quantifying phosphoethanolamine is provided, wherein an oxidoreductase is added to a sample containing phosphoethanolamine. A mediator can be reduced by adding the oxidoreductase, and the reduced mediator can be reacted with a reagent to determine the concentration of the phosphoethanolamine. Alternatively, as the oxidoreductase, hydrogen peroxide generated by adding an oxidase can be reacted with a reagent to determine the concentration of the phosphoethanolamine.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

Disclosed herein are methods, devices and kits suitable for high throughput screenings of extracellular pyridine nucleotide levels, such as NAD+ levels which are suitable for monitoring pyridine nucleotide induced slowdown of not only pathogenesis of multiple systemic diseases but also aging. In particular, assaying methods quantifying extracellular pyridine nucleotide(s), such as NAD+, in the low micromolar to the low nanomolar range in a sample that may have been subjected to long term storage are disclosed using a two-step enzymatic cycling reaction employing an oxidoreductase such as alcohol dehydrogenase. A modified revised simulated body fluid is also disclosed that is employed as a standard matrix to optimise enzymatic activity, linearity and / or sensitivity of the methods, devices and kits.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.