62 results about "Oxidoreductase" patented technology

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The present disclosure relates to a method of forming electrical signal sensor. The method includes the following operations. An oxidoreductase and an amphiphilic molecule are dissolved into a solvent to form a solution, in which the amphiphilic molecule includes alkyl sulfuric acid, alkyl sulfate, alkyl sulfonic acid, alkyl sulfonate, alkyl ammonium, alkyl ammonium salt, alkyl phosphoric acid, alkyl phosphate, alkyl carboxylic acid, alkyl carboxylate, alkylboronic acid, alkylborate, or combinations thereof. The solution is coated on an electrode layer. The solution is dried.

The application provides a human Helicobacter pylori detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology, amplifies the genomic fragment containing the four-strand DNA sequence in the human Helicobacter pylori genome, and realizes rapid and visual detection of the human Helicobacter pylori by adding hematin and oxidoreductase substrate for color reaction.

The invention belongs to the field of microorganism application, and particularly relates to a bacterium capable of degrading pyrethroid pesticides, and a preparation and application thereof. The invention relates to the field of biotechnology, and in particular relates to a pseudomonas aeruginosa, which is named as paracoccus panotrophus BB-2 and is preserved in the China Center for Type Culture Collection on May 26, 2025 with the preservation number of CCTCC NO: M 20251184, and pseudomonas aeruginosa, which is named as pseudomonas aeruginosa BS-3 and is preserved in the China Center for Type Culture Collection on May 26, 2025 with the preservation number of CCTCC NO: M 20251185. The invention further relates to a mixed bacterial agent of the paracoccus pantotrophus BB-2 and the pseudomonas aeruginosa BS-3, it is found that the paracoccus pantotrophus BB-2 and the pseudomonas aeruginosa BS-3 can be subjected to synergistic symbiosis and secrete and produce extracellular hydrolase and multiple oxidoreductases, pyrethroids are efficiently degraded, and the removal efficiency of the multiple pyrethroids reaches 90% or above.

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.

Process for the production of 5-hydroxymethyl-2-furancarboxylic acid (HMFA), wherein 5-hydroxymethylfurfural (HMF), which is present in an aqueous solution, is oxidized to obtain 5-hydroxymethyl-2-furancarboxylic acid (HMFA) by treatment with an NAD(P)+-dependent aldehyde dehydrogenase in vitro with NAD(P)H being formed; after said oxidation step the NAD(P)H formed during the oxidation is enzymatically regenerated with an oxidoreductase back to NAD(P)+, and a keto compound is used as a cosubstrate of the oxidoreductase.

The application provides a porcine parvovirus detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology to amplify the genomic fragment containing the four-strand DNA sequence in the porcine parvovirus genome, and realizes rapid and visual detection of porcine parvovirus by adding hematin and oxidoreductase substrate for color reaction.

This invention discloses a Bio-Fenton-driven in-situ polyacrylamide injection fluid depolymerization composition, its preparation method, and its application. The composition comprises an oxidoreductase, reducing sugar, and a transition metal ion source. In use, the composition is added to the injection fluid. The oxidoreductase catalyzes the in-situ oxidation of the reducing sugar to generate hydrogen peroxide, and the transition metal ions from the transition metal ion source mediate a Bio-Fenton reaction, generating highly reactive hydroxyl radicals that attack the polyacrylamide backbone, causing it to break and depolymerize. The final depolymerization viscosity can be reduced to below 5 mPa·s. The composition of this invention works effectively within the temperature range of 45–90°C and pH range of 4.0–10.0. The depolymerization process is stable and controllable. All components and degradation products are non-toxic and biodegradable. All components are in solid form, making storage and transportation safe and convenient.

An immobilized enzyme fiber contactor includes a plurality of fibers disposed within a hollow conduit. The fibers have attached thereto an enzyme selected from the group consisting of an oxidoreductase, a transferase, a hydrolase, a lyase, an isomerase or a ligase. The enzyme may be attached to the fiber via an anchor group and optionally a bifunctional crosslinking agent. The enzyme may be applied, stripped, and re-applied without detaching the reactor or discarding the fibers. The immobilized enzyme fiber contactor can be used for carrying out a two-phase or single-phase enzymatic reaction.

Disclosed herein are methods, devices and kits suitable for high throughput screenings of extracellular pyridine nucleotide levels, such as NAD+ levels which are suitable for monitoring pyridine nucleotide induced slowdown of not only pathogenesis of multiple systemic diseases but also aging. In particular, assaying methods quantifying extracellular pyridine nucleotide(s), such as NAD+, in the low micromolar to the low nanomolar range in a sample that may have been subjected to long term storage are disclosed using a two-step enzymatic cycling reaction employing an oxidoreductase such as alcohol dehydrogenase. A modified revised simulated body fluid is also disclosed that is employed as a standard matrix to optimise enzymatic activity, linearity and / or sensitivity of the methods, devices and kits.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The present application has an object to provide a novel protein cross-linking method. By allowing both an oxidoreductase such as laccase and a protein deamidase such as protein glutaminase to act on a substrate protein, a cross-linking reaction is promoted.

The invention discloses recombinant escherichia coli for producing D-tagatose by using redox enzyme driven by cofactor regeneration as well as a construction method and application of the recombinant escherichia coli, and belongs to the technical field of biological engineering. The method comprises the following steps: introducing xylose reductase xyrB, glucose dehydrogenase Gox2015, galactitol dehydrogenase RlGDH mutant T193G / G98C and water-producing NADH oxidase SpNox into escherichia coli, so as to construct recombinant escherichia coli; according to the method disclosed by the invention, a way for synthesizing the D-tagatose by regenerating and driving oxidoreductase through cofactors is designed and constructed, and lactose in whey can be completely converted into the D-tagatose and sodium gluconate by combining the way with beta-galactosidase, so that high-valued utilization of a dairy product processing by-product whey is realized.

The application belongs to the technical field of genetic engineering. The application provides application of a thiol-oxidoreductase in promoting keratin enzymolysis and a method for promoting keratin enzymolysis. The thiol-oxidoreductase is applied to a keratin enzymolysis system, and the enzymolysis effect is obviously improved. Through testing, the enzymolysis effect of the thiol-oxidoreductase combined with a protease on keratin is not only obviously higher than the enzymolysis effect of a single protease, but also is better than the hydrolysis of keratin by a chemical reducing agent with the same concentration. The application retains the advantages of enzyme method "green chemistry" and is friendly to the environment.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel oxidoreductase variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The oxidoreductase variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting oxidoreductase to change the enzymatic activity of the oxidoreductase, and a recombinant microorganism comprising the oxidoreductase variant is capable of efficiently producing L-glutamic acid.

This application provides a method and kit for detecting pathogenic microorganisms based on the properties of tetrastranded DNA oxidoreductase. The method utilizes isothermal amplification technology to amplify genomic fragments containing tetrastranded DNA / RNA sequences in the pathogenic microorganism genome, and then performs a colorimetric reaction by adding heme and oxidoreductase substrates, achieving rapid and visual detection of pathogenic microorganisms.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The present invention relates to the technical field of microbial metabolic engineering, and in particular to a Corynebacterium glutamicum strain capable of efficiently producing D-pantothenic acid, a construction method therefor, and the use thereof. The construction method specifically comprises the following steps: (1) using wild-type Corynebacterium glutamicum ATCC 13032 as a chassis, attenuating ilvA and performing mutation expression so as to construct DPA1; (2) knocking out pyruvate quinone oxidoreductase and inserting a panBCE gene expression cassette into same so as to construct DPA2; (3) introducing into the DPA2 an acetohydroxy acid synthase, an acetolactate isomeroreductase and a 2-hydroxyacid dehydratase which are overexpressed by using a pEC-xk99E overexpression plasmid and derived from Escherichia coli W3110 and Bacillus subtilis 168, and are endogenous and are obtained by screening; and (4) under the control of a strong promoter, subjecting the selected ilvBNCD gene to tandem overexpression by means of pEC-xk99E to construct DPA3, so that by enhancing the driving force in a principle synthesis pathway of pantothenic acid and upstream pathways, the Corynebacterium glutamicum strain capable of efficiently producing D-pantothenic acid is obtained. The strain provided by the present invention can use carbon sources to produce D-pantothenic acid with a high yield and high conversion rate.

The invention belongs to the technical field of synthetic biology and metabolic engineering, and particularly relates to a foreign protein expression system with low-degree interference on normal metabolism of a host as well as construction and application of the foreign protein expression system. The system is based on an orthogonal ribosome-mRNA pairing mechanism, an orthogonal SD sequence with complementarity lower than 20% with a host natural anti-SD sequence is obtained through design and screening, a candidate sequence with the host growth inhibition rate lower than 5% is screened from the orthogonal SD sequence, and the optimal orthogonal SD sequence and an orthogonal ASD sequence completely complementary with the optimal orthogonal SD sequence are determined. The system is suitable for high-efficiency expression of esterase, nitrilase and NADPH dependent oxidoreductase, and the enzyme activities of whole cells are respectively improved by 2.5 times, 3.4 times and 2.8 times, so that the system can effectively solve the problems of translation resource competition, high metabolic load, strong protein toxicity, inclusion body formation and the like in the foreign protein expression process; and a universal and stable technical platform is provided for efficient soluble expression of industrial enzymes.

The invention also relates to a method for preparing 2, 5-furandicarboxylic acid. The invention relates to a method for the in vitro oxidation of 5-formyl-2-furancarboxylic acid present in an aqueous solution to 2, 5-furandicarboxylic acid by treatment with an NAD (P) +-dependent oxidoreductase, in which NAD (P) H generated during the oxidation is enzymatically oxidized back to NAD (P) + by means of a dehydrogenase; and then removing the enzyme.

This invention discloses a system, method, and reagent kit for detecting biochemical oxygen demand (BOD) in wastewater, belonging to the field of environmental monitoring technology. The detection system of this invention includes at least two independent detection channels and a signal processing module. Each detection channel independently includes an enzymatic conversion module, which contains hydrolytic enzyme systems, oxidoreductase systems, and coenzyme NAD for specific types of organic matter. + The signal transduction and amplification module includes a CRISPR-Cas effector protein, a fluorescent reporter probe, and a molecular switch. The signal processing module detects the fluorescence signal intensity of each detection channel and converts the fluorescence signal into the biochemical oxygen demand (BOD) contribution value of each detection channel based on a pre-stored standard curve, summing them to obtain the total BOD. This invention effectively overcomes the detection uncertainty caused by fluctuations in microbial activity and differences in substrate degradation rates in traditional methods, significantly shortening the detection cycle to within 30 minutes.

Provided herein are methods and host cells for producing a polypeptide containing two chains, such as an antibody, half-antibody, antibody fragment, or one-armed antibody. The methods and host cells allow for two-chain polypeptide production using expression of polynucleotides encoding the polypeptide chains from extra-chromosomal polynucleotide(s), and expression of one or more chaperone protein(s) (e.g., peptidyl-prolyl isomerases and / or protein disulfide oxidoreductases) from the host cell chromosome using non-native combination(s) of promoters and translational units encoding a chaperone protein.

The present application belongs to the field of microbial fermentation and its metabolites, and particularly relates to a fermentation method and product composition for simultaneously producing superoxide dismutase and nano silver particles. The present application utilizes the stress tolerance characteristics of Deinococcus radiodurans, controls the concentration of Ag+, improves the enzyme activity of the oxidoreductase of Deinococcus radiodurans including SOD, and at the same time, Ag+ is reduced into nano silver particles (DR-Ag NPs). After the stress of Ag+ on Deinococcus radiodurans, the product is rich in oxidoreductase including SOD and nano silver particles (DR-Ag NPs), and after compounding with hyaluronic acid and the like, a functional cosmetic product can be prepared, which has super strong anti-aging, anti-wrinkle, whitening, antibacterial and anti-inflammatory effects, and at the same time, the use of preservatives is avoided.

The invention discloses an application of stevioside in preparation of drugs for preventing immunological liver injury, and experimental results show that administration of stevioside with different concentrations can significantly reduce ALT, AST and ALP levels in mouse serum, inhibit expression of TNF-alpha, IL-6 and IL-1beta, alleviate liver tissue injury and inflammatory cell infiltration, and inhibit oxidative stress. Liver transcriptome GO enrichment analysis finds that differential expression genes are related to oxidoreductase activity. Gene knockout is carried out on the NRF2, so that the protection effect of stevioside on the liver is obviously weakened. The invention discloses the protection effect of stevioside in an immunological liver injury model for the first time, and provides a new safe, natural and effective prevention strategy for immunological liver injury.