39 results about "Electron mediator" patented technology

The invention relates to the technical field of biological denitrification, in particular to a nitrogen-doped carbon dot-based microbial electronic control denitrification system and application thereof. The system comprises electron donor microorganisms, electron acceptor microorganisms and nitrogen-doped carbon dots, the nitrogen-doped carbon dots serve as electron mediators outside cells, an efficient extracellular electron transfer network is constructed, and interspecific electron transfer resistance is reduced; meanwhile, the nitrogen-doped carbon dots can regulate and control intracellular electron transport chains and the energy metabolism process, the improvement of the reducing power and the energy level is promoted, and the metabolism mode optimization is realized. By cooperatively regulating and controlling generation, transmission and utilization of electrons under an extreme electron donor limited oligotrophic condition, the system disclosed by the invention can realize efficient denitrification, avoid nitrite accumulation, remarkably inhibit generation of nitrous oxide (N2O), reduce dependence on an external organic carbon source and an electron source, and improve the denitrification efficiency. And a new technical approach is provided for improving the deep denitrification efficiency under the low-carbon condition.

The invention relates to a CdS / denitrifying bacteria hybrid system as well as a construction method and application thereof, and relates to the technical field of water nitrate pollution treatment. Comprising composite denitrifying bacteria, Zn-doped CdS heterojunction nanoparticles loaded on the surface of the composite denitrifying bacteria, and an electronic mediator anchored at the interface of the heterojunction nanoparticles and a flora, the composite denitrifying bacteria are prepared from Stutzeria balearica CXK-1 and Stutzeria balearica GUA-1 according to the volume ratio of the Stutzeria balearica CXK-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 being 1 to (0.8 to 1.2). The hybrid system can utilize photoelectrons to drive microbial denitrification under an illumination condition, so that the reduction of nitrate radicals is realized, and the effect of water treatment is achieved.

The invention belongs to the technical field of coal mine machinery lubrication and hydraulic transmission, and discloses an environment-friendly total-synthesis concentrated solution for a hydraulic support and a preparation method of the concentrated solution. The biological sensing microcapsule corrosion inhibitor is characterized by comprising 0.5%-3.0% of a biological sensing microcapsule, 8.0%-15.0% of a main corrosion inhibitor, 2.0%-6.0% of an auxiliary corrosion inhibitor, 4.0%-10.0% of a water-soluble lubricant, 0.05%-0.2% of an anti-foaming agent, 1.0%-3.0% of a pH buffering agent and deionized water. Wherein the biological sensing microcapsule has a core-shell-shell structure, is integrated with electroactive microorganisms, an electron mediator carrier and a corrosion inhibition precursor release layer, and can trigger targeted release of benzotriazole derivatives when the pH value of a local corrosion microarea is less than or equal to 5.5. The corrosion inhibitor has excellent corrosion protection performance and environment-friendly characteristics, effectively overcomes the problem of inactivation of microorganisms under severe hydraulic working conditions through a microcapsule encapsulation strategy, realizes accurate matching of corrosion signal in-situ sensing and corrosion inhibition response, remarkably improves the anti-rust performance under high-shear and wide-temperature-range working conditions, and has wide application prospects. And meanwhile, the requirements of environmental protection and sealing material compatibility are met.

A novel photocatalyst Bi2WO6 / NiO / Ag with hierarchical flower-like Z-scheme heterojunction, which exhibited excellent stability and photocatalytic activity over a wide light spectrum, was synthesized. The as-prepared composites were used in the remediation of real oil sands process water (OSPW) and achieved complete removal of aromatics, classical naphthenic acids (NAs). and heteroatomic NAs after 6 h of photocatalytic treatment. The acute toxicity of OSPW was completely eliminated after only 2 hours of treatment. h+, ·OH and O2·—were found to be the major oxidative species in the photocatalytic system. The enhanced photocatalytic efficiency is the result of the unique Z-scheme electron transfer among electron mediator Ag, NiO, and Bi2WO6 and the SPR effect near Ag, which was supported by the DFT calculations of the electronic properties of Bi2WO6 / NiO / Ag heterostructure.

This invention discloses a photocatalyst with a dual heterojunction structure, its preparation method, and its application. First, a solution containing iodide and a solution containing bismuth salt are mixed and the pH is adjusted to alkaline. A hydrothermal reaction is then carried out at 150–200°C. The resulting product is washed and dried to obtain product BiOI. Next, the prepared BiOI is calcined at 350–420°C to obtain product BiOI / Bi5O7I. Then, BiOI / Bi5O7I and Mn3O4 are dissolved in a first organic solvent, followed by a hydrothermal reaction at 150–200°C. The resulting product is washed and dried to obtain product Mn3O4 / BiOI / Bi5O7I. This invention introduces a Mn3O4 / BiOI / Bi5O7I dual heterojunction constructed using Mn3O4 as a redox electron mediator, ensuring that the photocatalyst maintains a suitable valence band position, thereby maintaining a strong redox reaction capability. Therefore, it can effectively remove NO and inhibit the formation of NO2 toxic byproducts.

This invention relates to the field of biological denitrification technology, and more particularly to a microbial electron-regulated denitrification system based on nitrogen-doped carbon dots and its application. The system comprises electron-donating microorganisms, electron-accepting microorganisms, and nitrogen-doped carbon dots. The nitrogen-doped carbon dots act as extracellular electron mediators, constructing an efficient extracellular electron transport network and reducing interspecies electron transport resistance. Simultaneously, the nitrogen-doped carbon dots can regulate intracellular electron transport chains and energy metabolism processes, promoting increased reducing power and energy levels, and optimizing metabolic patterns. By synergistically regulating electron generation, transport, and utilization under oligotrophic conditions with limited electron donors, this system achieves highly efficient denitrification, avoids nitrite accumulation, and significantly inhibits nitrous oxide (N2O) generation, while reducing dependence on external organic carbon sources and electron sources. This provides a new technical approach for improving the efficiency of deep denitrification under low-carbon conditions.

The present invention relates to the field of sludge treatment technology, and in particular to a process for promoting sludge hydrolysis and medium-chain fatty acid synthesis using ferrocene, comprising: S10, adding ferrocene to the sludge and adjusting the pH to 3.0-5.0; S20, adding hydrogen peroxide to carry out a Fenton-like reaction, wherein the ferrocene undergoes a solid-liquid phase transition during the reaction; S30, adding ethanol, anaerobic seed sludge, and culture medium to carry out anaerobic fermentation and carbon chain extension; ferrocene generates hydroxyl radicals in the Fenton-like reaction to destroy sludge flocs and cell wall membranes; the converted liquid ferrocene cation acts as an electron mediator in step S30 to improve electron transfer efficiency; the liquid ferrocene cation simultaneously inhibits ethanol oxidation and methanogenesis. The present invention combines the Fenton-like reaction with a biological anaerobic process to construct a sludge hydrolysis, fermentation, and carbon chain extension process based on ferrocene regulation, thereby simultaneously promoting sludge hydrolysis, enhancing medium-chain fatty acid synthesis, and inhibiting substrate competition reactions, thereby expanding the high-value recovery rate of sludge resources.

The present application belongs to the technical field of organic waste anaerobic fermentation, and discloses a method for producing medium-chain fatty acids by using blue vitriol to promote residual sludge anaerobic fermentation, which uses residual activated sludge from a municipal sewage treatment plant as a fermentation substrate, inoculates anaerobic sludge, adds blue vitriol and a methanogenesis inhibitor, adjusts pH value, and carries out anaerobic fermentation to produce medium-chain fatty acids under the participation of an electron donor; due to the addition of blue vitriol, the electron transfer capacity of microorganisms in the carbon chain extension process can be strengthened, thereby improving the yield of medium-chain fatty acids in the fermentation product; the addition amount of blue vitriol in the system is 2-20 g / L. By introducing blue vitriol into the sludge medium-chain fatty acid production system and strictly controlling the addition amount of blue vitriol in the system, the present application uses blue vitriol as an electron mediator to strengthen the electron transfer efficiency of microbial fermentation in the sludge medium-chain fatty acid production process, thereby improving the yield of medium-chain fatty acids, and further realizing efficient utilization of sludge resources.

The invention discloses an aqueous zinc ion battery electrolyte for promoting rapid activation and an aqueous zinc ion battery, and relates to the technical field of battery electrolytes. The electrolyte comprises a water solvent, a soluble zinc salt, a soluble manganese salt and persulfate serving as an additive, the molar concentration of the persulfate is 0.001-0.5 mol / L, and the molar concentration of the soluble manganese salt is 0.01-3 mol / L. The persulfate can be selectively adsorbed on the surface of an active site of manganese oxide, and is used as an electron mediator to promote electrochemical regeneration of an ineffective active site in the manganese-based positive electrode material; and meanwhile, manganese ions in the electrolyte can be induced to generate directional deposition on the surface of the positive electrode, so that the repair and supplement of active substances are realized. Through cooperation of the dual effects, the capacity increasing rate of the aqueous zinc ion battery in the initial activation stage is remarkably increased, the battery capacity rapidly reaches the highest stable value, and therefore the comprehensive electrochemical performance of the aqueous zinc ion battery is effectively improved.

The application provides a method for enhancing electricity generation of a microbial fuel cell by modifying the interface between a free magnetic carbon three-dimensional biological anode and bacteria, and the method adopts a solvothermal method to prepare a hydrophilic magnetic nano Fe3O4@ formed porous carbon composite material which has strong electromagnetic performance, a large specific surface area and high biocompatibility, can be used as a three-dimensional biological anode in a microbial fuel cell (MFC), is beneficial to the adhesion of microorganisms and maximizes the colonization space of the microorganisms, can promote the extracellular electron transfer efficiency of bacteria and enhance interspecies electron transfer between bacteria and archaea as an excellent electron medium, improves the conversion rate of a biological anode and the output of electrons, and enhances electricity generation of the MFC.

The invention discloses heat-resistant glucose dehydrogenase and application thereof in an enzyme fuel cell, and belongs to the crossing field of bioelectrochemistry and new energy technologies. According to the invention, firstly, a heat-resistant glucose dehydrogenase TeGDH is screened, and the heat-resistant glucose dehydrogenase TeGDH is subjected to heterologous expression in escherichia coli BL21 (DE3) and shows good heat stability and catalytic activity; teGDH is dissolved in an anolyte containing an electron mediator anthraquinone-2, 6-disulfonic acid disodium salt, and the heat-resistant high-energy enzyme fuel cell is prepared by adopting a non-immobilized system; the maximum power density of the battery at 50 DEG C reaches 1.74 mW / cm, and the battery has good thermal stability and long-term operation performance; the invention combines big data mining, enzyme engineering and electrochemical technologies, provides a novel enzyme fuel cell solution with high activity, high stability and low cost, and has a good application prospect.

The present application belongs to the technical field of medical equipment, and relates to an electrochemical biosensor for metabolite detection equipment. The sensor has: (1) an electrode system comprising a working electrode and a counter electrode; (2) a reaction reagent arranged on the working electrode and the counter electrode. The reaction reagent comprises: (a) an enzyme; (b) an electron mediator composition; (c) a buffer solution; wherein the electron mediator composition comprises a ruthenium complex and an aniline derivative. The sensor of the present application adopts the same biosensing electrochemical technology route, and as long as the enzyme in the reaction reagent is replaced, electrochemical detection of corresponding metabolites such as blood lactic acid, blood ketone and blood glucose can be realized, which is beneficial to production quality control and improvement of product performance. The sensor of the present application has the advantages of simple manufacturing process, large detection range and rapid and accurate detection.

This invention provides an electrochemical glucose sensor for continuous blood glucose monitoring, belonging to the field of biochemical sensing technology. The sensor includes a substrate and a three-electrode system, wherein the three-electrode system includes a working electrode, a reference electrode, and an auxiliary electrode. The working electrode comprises a conductive substrate, and an electrocatalytic enzyme composite layer and an outer membrane layer sequentially modified on the surface of the conductive substrate, with the outer membrane layer covering the outside of the electrocatalytic enzyme composite layer. The electrocatalytic enzyme composite layer is composed of glucose oxidase, an electron mediator, and a cross-linking agent. The glucose oxidase and the electron mediator are cross-linked and fixed to the surface of the conductive substrate by the cross-linking agent. This invention solves the technical problems of traditional electrochemical glucose sensors, such as long detection time, limited linear response range, low sensitivity and high detection limit, poor stability due to short-term signal drift, weak resistance to interference from sugar impurities and endogenous electroactive small molecules, and poor linearity and large detection error when directly detecting blood samples.

Provided is a new metallocene compound with a formal electron count of 20 to 22. The metallocene compound may be represented by formula (1). This application also provides methods for producing the compound, which can be used, amongst other things, as a catalyst, an electron mediator, or as an electrolyte in a redox flow battery. The unique electronic structure of these compounds provides for novel and highly tunable redox properties.

The invention discloses a magnetic field modulated electrochemical detection method for horseradish peroxidase, and belongs to the field of electrochemical detection. According to the method, a magnetic field is used for controlling horse radish peroxidase taking magnetic beads as carriers to be in contact with the surface of an electrode of a modified enzyme substrate as required, and ultra-low-amount horse radish peroxidase detection is completed through a time domain detection method and a frequency domain detection method. In time domain detection, an enzyme substrate is fixed on the surface of an electrode, and enzyme is controlled to be far away from and contact with the surface of the electrode through a magnetic field, so that an accurate response signal for shielding noise is obtained. In order to detect a lower amount of enzyme, the magnetic beads are controlled to be in periodic contact with the surface of the electrode by externally adding a periodically changing magnetic field, so that a periodic electrochemical response signal with frequency characteristics is formed. The detection method can be applied to an electrochemical sensing detection system taking horse radish peroxidase or an enzyme-labeled biomarker as a detection object or an electronic mediator, and the detection limit of a traditional horse radish peroxidase electrochemical detection method is improved.

A UiO-66-NH2 / Pt / UiO-66-NH2 catalyst, its preparation, and its application belong to the field of photocatalytic reduction of CO2. It has a "sandwich" structure: defective UiO-66-NH2 serves as the active component substrate, with a noble metal interlayer of Pt acting as the photocatalytic active center, and UiO-66-NH2 as the outer layer. Pt nanoparticles act as electron mediators, thereby efficiently improving photocatalytic activity. The close contact between the highly dispersed Pt and UiO-66-NH2 shortens the electron transport distance and promotes interfacial charge transfer. Furthermore, UiO-66-NH2 not only inhibits Pt aggregation and leaching but also provides more active sites for the reaction, enabling the material to exhibit excellent activity and stability in CO2 reduction under visible light.

The invention relates to the field of organic synthesis and molecular medicine, in particular to a preparation method of a phenazine derivative electron mediator 1-methoxy-5-methylphenazine methyl sulfate salt (1-methoxyl PMS) and application of the phenazine derivative electron mediator 1-methoxy-5-methylphenazine methyl sulfate salt in the electron mediator and a sensor. According to the synthesis method, o-vanillin is taken as a starting raw material, 1-methoxyphenazine is synthesized through m-chloroperoxybenzoic acid oxidation (a solvent-free system), sodium periodate water-phase oxidation and alkaline aqueous solution coupling in sequence, and then methylation reaction is performed in a tetrahydrofuran solvent to prepare a target product. According to the method, a water / THF dual-phase system is innovatively adopted, a'one-pot method 'oxidative coupling simplified process is adopted, acidification pulping replaces traditional column chromatography purification, and a noble metal catalyst is not needed in the whole process. The total yield reaches 60%, the heavy metal residue of the product is less than 1ppm, the solvent toxicity is reduced by 90%, the reaction condition is mild (room temperature / normal pressure), and a new green manufacturing scheme is provided for localization of the CCK-8 kit.

The application relates to the technical field of microbial remediation methods, and specifically discloses an ex-situ microbial remediation method for river sewage, aiming to solve the multiple contradictions of redundancy of existing ex-situ microbial remediation technology, single function of bacterial flora, weak environmental adaptability and difficulty in giving consideration to high efficiency and engineering simplicity. The method comprises the following steps: guiding the river sewage out and pretreating the river sewage to remove large-particle impurities; introducing a biological reactor to inoculate a composite functional bacterial flora composed of the genus Raoultella, the genus Pseudomonas and the genus Bacillus in proportion; implementing gradient dissolved oxygen regulation to realize simultaneous nitrification and denitrification; adding iron-manganese modified biochar as a microbial carrier and an electron mediator; and recycling the biochar after solid-liquid separation and reaction. Through the above technical scheme, the application can realize multi-target and simultaneous high-efficiency removal of ammonia nitrogen, refractory organic matter and trace heavy metals, simplify a process flow, improve system stability and low-temperature adaptability, and support modular deployment and intelligent operation and maintenance.

The invention discloses an LDHA enzyme activity detection method based on an MTS color development system. According to the method, a standard substance and a sample to be detected are respectively mixed and reacted with a buffer solution containing excessive pyruvic acid and NADH, then MTS and a phenazine electron mediator are added into a reaction system, and residual NADH is reduced into formazan capable of absorbing visible light at 490 nm, so that weak NADH change is amplified into an end point signal which is easy to detect and high in reproducibility, and the detection sensitivity is high. And substituting the end point signal of the to-be-detected sample into a standard curve constructed by the enzyme activity of the standard substance and the end point signal of the standard substance to obtain the LDHA enzyme activity of the to-be-detected sample. According to the method, the detection sensitivity of LDHA can be improved by about 2-3 times, background interference is remarkably reduced, the method is suitable for high-throughput drug screening and large-scale sample detection, and a sensitive and reliable detection means with industrialization potential is provided for the fields of tumor metabolism research, LDHA targeted drug screening, clinical metabolism marker detection and the like.

To provide test paper for amino acid quantitation with superior color development stability in storage.SOLUTION: Test paper of the present invention is for quantitating amino acid concentration in a test sample by a colorimetric method, and comprises a liquid-permeable carrier carrying a dehydrogenase that catalyzes a dehydrogenation reaction of amino acids, a coenzyme, an electron mediator, a reductive chromogenic reagent, and a stabilizer.SELECTED DRAWING: None

The invention relates to the technical field of battery treatment, solves the problem of low microbial metabolism efficiency caused by local microenvironment imbalance in the existing waste lead-acid storage battery lead plaster biological hydrometallurgy process, and particularly relates to a hydrometallurgy recovery method for lead plaster in a waste lead-acid storage battery. Comprising the following steps: acquiring structural parameters of a reaction container, characteristic parameters of lead plaster and physiological parameters of microorganisms, and deducing a temporal and spatial change rule of a metabolic rate in an undisturbed state; a full-dimensional monitoring network is constructed in the reactor, and spatial and temporal distribution of microbial metabolism key parameters is captured in real time. Through a dynamic precise regulation and control strategy based on root discrimination, micro-bubble cleaning and local turbulence can be adaptively optimized for physical contact failure, and missing protons and electron mediators can be accurately calculated and injected for chemical environment imbalance, so that the spanning from extensive full-field intervention to micro-region-level precise repair is realized, and the method has the advantages of simple operation, low cost and high efficiency. And finally, the leaching rate and the recovery efficiency of the lead plaster are remarkably improved.

The invention discloses a method for removing florfenicol by using an iron-doped biochar reinforced microbial electrolysis cell, which is characterized in that florfenicol in a water body is removed by using the microbial electrolysis cell, and a carbon-based material loaded with iron-doped biochar is used as a cathode in the microbial electrolysis cell. In the invention, the carbon-based material loaded with the iron-doped biochar is used as the cathode, so that the specific surface area and roughness of the electrode can be remarkably increased, florfenicol can be degraded by utilizing different functional microorganisms, and the carbon-based material can be used as an electron mediator to promote extracellular electron transfer between the cathode and the microorganisms, so that the efficient removal of florfenicol is facilitated; the method is used for reducing and degrading florfenicol in a water body, can quickly reduce florfenicol into low-toxicity products, can realize quick removal of florfenicol, has the advantages of simple process, low cost, low energy consumption, high treatment efficiency, good removal effect and the like, is a green technology capable of efficiently treating florfenicol-polluted wastewater, is high in use value and has wide application prospects. The application prospect is good.

An enzyme electrode that includes: an electrode support; an oxidoreductase; a conjugate of a silane coupling agent and an electron mediator; and a sol-gel matrix, wherein the oxidoreductase and the conjugate are fixed to the electrode support by the sol-gel matrix, and the silane coupling agent has a silicon atom, a reactive functional group, and a hydrolyzable group, and a structure in which the silicon atom and the reactive functional group are linked by a linking group having 4 or more carbon atoms.

An electrochemical biosensor including: an electrode; an electron mediator; and a biological enzyme; the electron mediator and the biological enzyme are disposed on a surface of the electrode, wherein the electron mediator comprises a transition metal complex directly bonded to the electrode via a chemical bond, and the biological enzyme is directly bonded to the electrode via a chemical bond or the biological enzyme is physically adsorbed onto the electrode.

This application discloses a redox complex and preparation method therefor and use thereof. The redox complex contains a bridged 2,2′-biimidazole structure, through bridging two nitrogen atoms with active hydrogen, stabilizing the structure of the imidazole ring, preventing conformational isomerism of the 2,2′-biimidazole. When the bridged 2,2′-biimidazole structure coordinates with transition metals to form a complex, which significantly improves the efficiency and stability of the complex. Therefore, when the redox complex is used as the electron mediator in sensors, it exhibits a lower oxidation potential, while significantly extending the service life of the sensors.

This invention belongs to the field of electrochemical sensor technology, specifically relating to an electrochemical sensor for continuous monitoring of lactic acid, its preparation method, and its application. The sensor includes a substrate electrode, a sensitive layer, and a diffusion confinement layer. The sensitive layer, coated on the surface of the substrate electrode, contains a lactic acid oxidation catalyst and an electron mediator. The diffusion confinement layer is a multilayer cross-linked polymer film or a multilayer non-cross-linked polymer film. The electrochemical sensor of this invention precisely controls the pore size and affinity of the membrane layer by adjusting the monomer ratio and graft modification of the diffusion confinement layer copolymer, achieving both high sensitivity and a wide linear detection range. It is highly adaptable and can meet the needs of different concentration ranges in medical monitoring, exercise physiological monitoring, etc. Simultaneously, the curing conditions simulate the human body environment, ensuring that the actual performance is consistent with laboratory tests, and its stability is reliable.

This invention provides a method for hydrogen production from organic wastewater through dark fermentation, belonging to the field of fermentation hydrogen production technology. The method includes: mixing organic wastewater, biochar loaded with metal nanoparticles, and a domesticated butyric acid-producing mixed bacterial community; performing micro-electrolysis and bioactivation treatment to obtain a pretreated solution; then subjecting the pretreated solution to hydrogen-producing phase fermentation treatment, adding an electron mediator to obtain a fermentation broth containing volatile acids; further, subjecting the fermentation broth to de-inhibition phase treatment, using adsorbent materials to adsorb volatile acids, and simultaneously converting some of the adsorbed volatile acids into butyric acid through acid-producing bacteria to obtain a post-fermentation solution; electrolyzing the post-fermentation solution, utilizing electroactive hydrogen-producing bacteria for extracellular electron transfer to convert residual organic matter in the solution into hydrogen gas to obtain crude hydrogen, which is then purified to obtain hydrogen gas. This invention solves the problems of low utilization rate of organic wastewater, inhibition of hydrogen production by volatile acids, and the impact of environmental parameter fluctuations on yield in existing processes.

The invention relates to the technical field of microbial synthesis, and provides a method for promoting microorganisms to reduce carbon dioxide to synthesize multi-carbon fatty acid, which comprises the following steps: in a reactor, inoculating an electroactive carbon chain in a microbial electrosynthesis system to prolong a microbial mixed flora, adding sodium bicarbonate, and aerating by using nitrogen and carbon dioxide to obtain the multi-carbon fatty acid. Applying external voltage to the reactor, and reacting to obtain the multi-carbon fatty acid; the inorganic substrate carbon dioxide is used as a unique carbon source, green electric energy is used as an energy source, conversion of carbon dioxide to fatty acid is promoted, additional organic electron donors or expensive electron mediators do not need to be added, the synthesis process of acetic acid generated by reduction of carbon dioxide to butyric acid and hexanoic acid is remarkably promoted, and the yield of fatty acid is increased. Good application prospects are realized.

The invention discloses an energy-saving and less-manganese-residue decoupling electrolytic manganese preparation system and method. The system comprises a cathode chamber and an anode chamber which are separated by a proton membrane, cathode electrolyte for manganese reduction is circularly introduced into the cathode chamber, and a reduction reaction for manganese deposition is carried out; an inert anode is adopted in the anode chamber, and a preferable oxidation-reduction medium is introduced to carry out rapid electrode reaction on the inert anode, so that oxygen is prevented from being separated out from the anode. According to the method disclosed by the invention, by virtue of a strategy of separating cathode and anode electrolytic bath liquid and introducing redox mediation on an anode side, ferricyanide / ferrocyanide is used as an electron mediator between anode half electrolytic baths, so that more favorable dynamic half reaction is realized, the bath voltage is effectively reduced, and the consumption of electric energy and the electrodeposition efficiency of manganese are greatly reduced; and the generation of manganese deposition byproducts is effectively eliminated.

An enzyme electrode (1) includes an electrode base material (2), an oxidoreductase (3), a conjugate (6) of an electron mediator (5) and a silane coupling agent (4) having a silicon atom, a reactive functional group, and a hydrolyzable group, the oxidoreductase (3) and the conjugate (6) being immobilized on the electrode base material (2) via the sol-gel matrix (7). And a structure in which the silicon atom and the reactive functional group are linked via a linking group having 4 or more carbon atoms.