64 results about "Electron mediator" patented technology

The invention discloses a landfill leachate anaerobic-denitrification treatment process and system based on waste molasses synergistic interaction, and belongs to the technical field of sewage treatment. The process comprises the following steps: (1) carrying out two-stage anaerobic digestion on the landfill leachate to remove organic matters and recover biogas, wherein first-stage anaerobic digestion adopts medium-temperature (35 + / -2 DEG C) anaerobic digestion, and second-stage anaerobic digestion adopts high-temperature (55 + / -1 DEG C) anaerobic digestion; (2) removing impurities from the waste molasses, diluting the waste molasses, mixing the diluted waste molasses with activated sludge, fermenting under an anaerobic condition to generate fermentation liquor rich in volatile fatty acid, and adding the fermentation liquor into a denitrification reactor; and (3) constructing an anaerobic-denitrification synergistic system, and increasing the denitrification rate by using the charcoal-loaded nano zero-valent iron (nZVI at BC) as an electronic mediator. According to the method, resource utilization of the waste molasses is realized, the problem of imbalance of the carbon nitrogen ratio of the landfill leachate is solved, anaerobic digestion and nitrification and denitrification processes are coupled, efficient nitrogen removal and organic matter removal are realized, and meanwhile, the sludge yield and the operation cost are reduced.

The invention relates to the technical field of biological denitrification, in particular to a nitrogen-doped carbon dot-based microbial electronic control denitrification system and application thereof. The system comprises electron donor microorganisms, electron acceptor microorganisms and nitrogen-doped carbon dots, the nitrogen-doped carbon dots serve as electron mediators outside cells, an efficient extracellular electron transfer network is constructed, and interspecific electron transfer resistance is reduced; meanwhile, the nitrogen-doped carbon dots can regulate and control intracellular electron transport chains and the energy metabolism process, the improvement of the reducing power and the energy level is promoted, and the metabolism mode optimization is realized. By cooperatively regulating and controlling generation, transmission and utilization of electrons under an extreme electron donor limited oligotrophic condition, the system disclosed by the invention can realize efficient denitrification, avoid nitrite accumulation, remarkably inhibit generation of nitrous oxide (N2O), reduce dependence on an external organic carbon source and an electron source, and improve the denitrification efficiency. And a new technical approach is provided for improving the deep denitrification efficiency under the low-carbon condition.

The invention belongs to the technical field of anaerobic fermentation of organic wastes, and discloses a method for producing medium-chain fatty acid by promoting anaerobic fermentation of excess sludge by using blue iron ore, which comprises the following steps: inoculating anaerobic sludge by using excess activated sludge from a municipal sewage treatment plant as a fermentation substrate, adding the blue iron ore and a methanogenesis inhibitor, adjusting the pH value, and fermenting to obtain the medium-chain fatty acid. Carrying out anaerobic fermentation in the presence of an electron donor to produce medium-chain fatty acid; due to the addition of the blue iron ore, the electron transfer capability of microorganisms in a carbon chain extension process can be enhanced, so that the yield of medium-chain fatty acid in a fermentation product is increased; the addition amount of the blue iron ore in the system is 2-20g / L. According to the method, the blue iron ore is introduced into a system for producing the medium-chain fatty acid from the sludge, the addition amount of the blue iron ore in the system is strictly controlled, the blue iron ore is used as an electron mediator, and the electron transfer efficiency of microbial fermentation in the process of producing the medium-chain fatty acid from the sludge is enhanced, so that the yield of the medium-chain fatty acid is increased; further, efficient utilization of sludge resources is realized.

The invention relates to a CdS / denitrifying bacteria hybrid system as well as a construction method and application thereof, and relates to the technical field of water nitrate pollution treatment. Comprising composite denitrifying bacteria, Zn-doped CdS heterojunction nanoparticles loaded on the surface of the composite denitrifying bacteria, and an electronic mediator anchored at the interface of the heterojunction nanoparticles and a flora, the composite denitrifying bacteria are prepared from Stutzeria balearica CXK-1 and Stutzeria balearica GUA-1 according to the volume ratio of the Stutzeria balearica CXK-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 being 1 to (0.8 to 1.2). The hybrid system can utilize photoelectrons to drive microbial denitrification under an illumination condition, so that the reduction of nitrate radicals is realized, and the effect of water treatment is achieved.

The invention belongs to the technical field of coal mine machinery lubrication and hydraulic transmission, and discloses an environment-friendly total-synthesis concentrated solution for a hydraulic support and a preparation method of the concentrated solution. The biological sensing microcapsule corrosion inhibitor is characterized by comprising 0.5%-3.0% of a biological sensing microcapsule, 8.0%-15.0% of a main corrosion inhibitor, 2.0%-6.0% of an auxiliary corrosion inhibitor, 4.0%-10.0% of a water-soluble lubricant, 0.05%-0.2% of an anti-foaming agent, 1.0%-3.0% of a pH buffering agent and deionized water. Wherein the biological sensing microcapsule has a core-shell-shell structure, is integrated with electroactive microorganisms, an electron mediator carrier and a corrosion inhibition precursor release layer, and can trigger targeted release of benzotriazole derivatives when the pH value of a local corrosion microarea is less than or equal to 5.5. The corrosion inhibitor has excellent corrosion protection performance and environment-friendly characteristics, effectively overcomes the problem of inactivation of microorganisms under severe hydraulic working conditions through a microcapsule encapsulation strategy, realizes accurate matching of corrosion signal in-situ sensing and corrosion inhibition response, remarkably improves the anti-rust performance under high-shear and wide-temperature-range working conditions, and has wide application prospects. And meanwhile, the requirements of environmental protection and sealing material compatibility are met.

A purification method for an electron mediator on a biosensor, the electron mediator having an amino. The purification method comprises the following steps: weighing an electron mediator with low purity; reacting an amino protection reagent with the electron mediator to obtain an amino-protected electron mediator, dissolving the amino-protected electron mediator in an organic solvent, and carrying out extraction to obtain an amino-protected electron mediator with high purity; and reacting the amino-protected electron mediator with high purity with a deprotection reagent for amino deprotection, thus obtaining an electron mediator with high purity. The method for purification achieves a very large treatment capacity in one time, effectively improves the purification efficiency of the electron mediator, and reduces the preparation cost. The electron mediator with high purity obtained from purification, serving as one of the components in the reagent on a biosensor, can be used for preparation of biosensors such as a blood glucose test biosensor.

A novel photocatalyst Bi2WO6 / NiO / Ag with hierarchical flower-like Z-scheme heterojunction, which exhibited excellent stability and photocatalytic activity over a wide light spectrum, was synthesized. The as-prepared composites were used in the remediation of real oil sands process water (OSPW) and achieved complete removal of aromatics, classical naphthenic acids (NAs). and heteroatomic NAs after 6 h of photocatalytic treatment. The acute toxicity of OSPW was completely eliminated after only 2 hours of treatment. h+, ·OH and O2·—were found to be the major oxidative species in the photocatalytic system. The enhanced photocatalytic efficiency is the result of the unique Z-scheme electron transfer among electron mediator Ag, NiO, and Bi2WO6 and the SPR effect near Ag, which was supported by the DFT calculations of the electronic properties of Bi2WO6 / NiO / Ag heterostructure.

This invention discloses a photocatalyst with a dual heterojunction structure, its preparation method, and its application. First, a solution containing iodide and a solution containing bismuth salt are mixed and the pH is adjusted to alkaline. A hydrothermal reaction is then carried out at 150–200°C. The resulting product is washed and dried to obtain product BiOI. Next, the prepared BiOI is calcined at 350–420°C to obtain product BiOI / Bi5O7I. Then, BiOI / Bi5O7I and Mn3O4 are dissolved in a first organic solvent, followed by a hydrothermal reaction at 150–200°C. The resulting product is washed and dried to obtain product Mn3O4 / BiOI / Bi5O7I. This invention introduces a Mn3O4 / BiOI / Bi5O7I dual heterojunction constructed using Mn3O4 as a redox electron mediator, ensuring that the photocatalyst maintains a suitable valence band position, thereby maintaining a strong redox reaction capability. Therefore, it can effectively remove NO and inhibit the formation of NO2 toxic byproducts.

The present invention relates to iron removal from silica sand, and specifically to a biological reduction method and system for iron removal from silica sand. By using Shewanella sp. bacteria to reduce crystalline iron, and simultaneously improving the iron removal rate through the synergistic effect of sodium lactate, sulfur-containing compounds and electron mediators, and accelerating the iron removal speed by washing with organic acids. Compared with the existing methods, the biological reduction method for iron removal from silica sand of the present invention can not only effectively remove crystalline iron in silica sand, but also recycle Shewanella sp. bacterial liquid and organic acid solution.

This invention relates to the field of biological denitrification technology, and more particularly to a microbial electron-regulated denitrification system based on nitrogen-doped carbon dots and its application. The system comprises electron-donating microorganisms, electron-accepting microorganisms, and nitrogen-doped carbon dots. The nitrogen-doped carbon dots act as extracellular electron mediators, constructing an efficient extracellular electron transport network and reducing interspecies electron transport resistance. Simultaneously, the nitrogen-doped carbon dots can regulate intracellular electron transport chains and energy metabolism processes, promoting increased reducing power and energy levels, and optimizing metabolic patterns. By synergistically regulating electron generation, transport, and utilization under oligotrophic conditions with limited electron donors, this system achieves highly efficient denitrification, avoids nitrite accumulation, and significantly inhibits nitrous oxide (N2O) generation, while reducing dependence on external organic carbon sources and electron sources. This provides a new technical approach for improving the efficiency of deep denitrification under low-carbon conditions.

The present invention relates to a method for improving the detection performance of an electrochemical biosensor and its application in a ratiometric electrochemical biosensor. Methylene blue and cholesterol-modified double-stranded DNA probes are immobilized on a gold electrode modified with a 1-hexanethiol assembly layer via hydrophobic interaction, thereby improving the stability of the methylene blue at the gold electrode interface and the immobilization efficiency of the double-stranded DNA probe at the gold electrode interface. In a potassium ferricyanide-mediated electrocatalytic system, the methylene blue on the gold electrode serves as a signal unit, and ferrocene is introduced as another signal unit. The 1-hexanethiol assembly layer on the gold electrode can shield [Fe(CN)6] 3‒ Electron transfer between the electrode interface and methylene blue enables simultaneous signal amplification of both methylene blue and ferrocene. Methylene blue, acting as an electron mediator, enhances the signal response of ferrocene, achieving secondary signal amplification of ferrocene, thereby improving sensor detection sensitivity. The ratiometric electrochemical biosensor constructed using this method exhibits excellent sensitivity, accuracy, and selectivity.

The present invention relates to the technical field of biochemistry, in particular to a composite enzyme solution for total cholesterol detection, a test paper and a detection system. The composite enzyme solution for total cholesterol detection comprises cholesterol oxidase, cholesterol esterase, peroxidase, an electron mediator, a surfactant composition, a thickening agent, a protective agent, a metal inorganic salt and a buffer solution, wherein the surfactant composition comprises one of or a combination of at least two of surfactants with an HLB>23 and one of or a combination of at least two of surfactants with an HLB of 12-14. The composite enzyme solution can be used for detecting total cholesterol by means of an electrochemical method, exhibiting fast detection speed, high sensitivity, and high accuracy.

The present invention discloses an α-Fe2O3 / RGO / mpg-C3N4 nanocomposite material and its preparation method and application, belonging to the field of materials science and technology. The mpg-C3N4 material is modified in terms of morphology control, element doping, and heterostructure construction, and a Z-type heterojunction photocatalyst with a solid electron mediator is synthesized based on the mpg-C3N4. By controlling the synthesis conditions, a large specific surface area, visible light response, non-toxic and environmentally friendly α-Fe2O3 / RGO / mpg-C3N4 nanocomposite material is prepared. This nanocomposite material overcomes the inherent shortcomings of single semiconductors g-C3N4 and α-Fe2O3, such as small specific surface area, low charge carrier separation efficiency, and limited visible light absorption capacity. The structure and properties of the nanocomposite material are characterized by XPS, XRD, SEM, TEM, FT-IR, BET, UV-vis DRS, and Zeta potential analysis, proving the successful preparation of the material. It was used to photocatalytically degrade the explosive 2,4,6-trinitrotoluene. A series of exploratory experiments showed that under 12 hours of illumination, the photodegradation rate of TNT by 10wt% α-Fe2O3 / RGO / mpg-C3N4 nanocomposite material can reach 99.57%, which is better than other materials and methods.

The present invention relates to the field of sludge treatment technology, and in particular to a process for promoting sludge hydrolysis and medium-chain fatty acid synthesis using ferrocene, comprising: S10, adding ferrocene to the sludge and adjusting the pH to 3.0-5.0; S20, adding hydrogen peroxide to carry out a Fenton-like reaction, wherein the ferrocene undergoes a solid-liquid phase transition during the reaction; S30, adding ethanol, anaerobic seed sludge, and culture medium to carry out anaerobic fermentation and carbon chain extension; ferrocene generates hydroxyl radicals in the Fenton-like reaction to destroy sludge flocs and cell wall membranes; the converted liquid ferrocene cation acts as an electron mediator in step S30 to improve electron transfer efficiency; the liquid ferrocene cation simultaneously inhibits ethanol oxidation and methanogenesis. The present invention combines the Fenton-like reaction with a biological anaerobic process to construct a sludge hydrolysis, fermentation, and carbon chain extension process based on ferrocene regulation, thereby simultaneously promoting sludge hydrolysis, enhancing medium-chain fatty acid synthesis, and inhibiting substrate competition reactions, thereby expanding the high-value recovery rate of sludge resources.

The invention relates to a preparation method and application of a phenanthroline-based Schiff base derivative, a Schiff base substance is synthesized by amine-aldehyde condensation reaction between 1, 10-phenanthroline-2, 9-dicarboxaldehyde (PDL) and thionine (Thi), the PDL has an excellent carbon skeleton, the Thi has an excellent electron transport performance, and the Schiff base substance can be used for preparing the phenanthroline-based Schiff base derivative. The two are combined to synthesize a novel electron mediator (PT) with good electron transmission performance and high stability. The preparation method is simple, efficient and low in cost, the current in the solution to be detected is tested through a chronoamperometry, and the concentration of the catechol in the solution to be detected is obtained according to the linear relation between the concentration of the catechol and the current. According to the invention, PT is used as an electron transporter, the composite material is formed by loading laccase through PT to serve as a catechol electrochemical sensing catalyst, the composite material shows excellent electrochemical sensing performance, catechol is detected under the constant overpotential condition of 0.23 V (vs.SCE), the lowest detection lower limit is 0.531 [mu] M, and the linear response range is 1-1000 [mu] M.

The invention relates to the technical field of sludge treatment, in particular to a process for promoting sludge hydrolysis and medium-chain fatty acid synthesis by using ferrocene, which comprises the following steps: S10, adding ferrocene into sludge and adjusting the pH value to 3.0-5.0; s20, hydrogen peroxide is added for a Fenton-like reaction, and ferrocene is subjected to solid-liquid form transformation in the reaction process; s30, adding ethanol, anaerobic seed sludge and a culture medium for anaerobic fermentation and carbon chain extension; the ferrocene generates hydroxyl radicals in the Fenton-like reaction to destroy sludge flocs and cell wall membranes; the converted liquid ferrocene cation is used as an electron mediator in the step S30 to improve the electron transfer efficiency; the liquid ferrocene cations simultaneously inhibit ethanol oxidation and methanogenesis processes. A Fenton-like reaction and a biological anaerobic process are combined, a sludge hydrolysis fermentation-carbon chain extension process based on ferrocene regulation and control is constructed, sludge hydrolysis promotion, medium-chain fatty acid synthesis enhancement and substrate competition reaction inhibition are synchronously realized, and the high-value recovery rate of sludge resources is increased.

The present application belongs to the technical field of organic waste anaerobic fermentation, and discloses a method for producing medium-chain fatty acids by using blue vitriol to promote residual sludge anaerobic fermentation, which uses residual activated sludge from a municipal sewage treatment plant as a fermentation substrate, inoculates anaerobic sludge, adds blue vitriol and a methanogenesis inhibitor, adjusts pH value, and carries out anaerobic fermentation to produce medium-chain fatty acids under the participation of an electron donor; due to the addition of blue vitriol, the electron transfer capacity of microorganisms in the carbon chain extension process can be strengthened, thereby improving the yield of medium-chain fatty acids in the fermentation product; the addition amount of blue vitriol in the system is 2-20 g / L. By introducing blue vitriol into the sludge medium-chain fatty acid production system and strictly controlling the addition amount of blue vitriol in the system, the present application uses blue vitriol as an electron mediator to strengthen the electron transfer efficiency of microbial fermentation in the sludge medium-chain fatty acid production process, thereby improving the yield of medium-chain fatty acids, and further realizing efficient utilization of sludge resources.

The invention discloses a heavy metal soil passivation remediation method for environmental protection, and belongs to the technical field of soil remediation. Aiming at the technical defects that the existing biochar is insufficient in adsorption capacity and zero-valent iron is easy to oxidize and agglomerate, a multi-mechanism synergistic remediation scheme taking the FeNi-coated TA-AC composite material as a core is provided. The surface of the zero-valent iron is coated with the porous activated carbon to form a core-shell structure, a carbon layer serves as a physical barrier to isolate oxygen and moisture and prevent the zero-valent iron from being oxidized, and the high specific surface area of the activated carbon and the high adsorbability of the zero-valent iron synergistically remove heavy metal; the surface of biochar is modified through tannic acid, tannic acid anchors activated carbon through polyphenol hydroxyl, and quinonyl of tannic acid serves as an electron mediator to accelerate heavy metal reduction; fe-Ni bimetal reduces surface energy through lattice distortion, and electron transfer is promoted through potential difference. The prepared composite material has obvious passivation efficiency on Pb, Cd and As, can be recycled after being recycled through a magnetic separation method, improves the material utilization rate, and has the advantages of being green and environmentally friendly.

The invention discloses an aqueous zinc ion battery electrolyte for promoting rapid activation and an aqueous zinc ion battery, and relates to the technical field of battery electrolytes. The electrolyte comprises a water solvent, a soluble zinc salt, a soluble manganese salt and persulfate serving as an additive, the molar concentration of the persulfate is 0.001-0.5 mol / L, and the molar concentration of the soluble manganese salt is 0.01-3 mol / L. The persulfate can be selectively adsorbed on the surface of an active site of manganese oxide, and is used as an electron mediator to promote electrochemical regeneration of an ineffective active site in the manganese-based positive electrode material; and meanwhile, manganese ions in the electrolyte can be induced to generate directional deposition on the surface of the positive electrode, so that the repair and supplement of active substances are realized. Through cooperation of the dual effects, the capacity increasing rate of the aqueous zinc ion battery in the initial activation stage is remarkably increased, the battery capacity rapidly reaches the highest stable value, and therefore the comprehensive electrochemical performance of the aqueous zinc ion battery is effectively improved.

The application provides a method for enhancing electricity generation of a microbial fuel cell by modifying the interface between a free magnetic carbon three-dimensional biological anode and bacteria, and the method adopts a solvothermal method to prepare a hydrophilic magnetic nano Fe3O4@ formed porous carbon composite material which has strong electromagnetic performance, a large specific surface area and high biocompatibility, can be used as a three-dimensional biological anode in a microbial fuel cell (MFC), is beneficial to the adhesion of microorganisms and maximizes the colonization space of the microorganisms, can promote the extracellular electron transfer efficiency of bacteria and enhance interspecies electron transfer between bacteria and archaea as an excellent electron medium, improves the conversion rate of a biological anode and the output of electrons, and enhances electricity generation of the MFC.

The invention discloses heat-resistant glucose dehydrogenase and application thereof in an enzyme fuel cell, and belongs to the crossing field of bioelectrochemistry and new energy technologies. According to the invention, firstly, a heat-resistant glucose dehydrogenase TeGDH is screened, and the heat-resistant glucose dehydrogenase TeGDH is subjected to heterologous expression in escherichia coli BL21 (DE3) and shows good heat stability and catalytic activity; teGDH is dissolved in an anolyte containing an electron mediator anthraquinone-2, 6-disulfonic acid disodium salt, and the heat-resistant high-energy enzyme fuel cell is prepared by adopting a non-immobilized system; the maximum power density of the battery at 50 DEG C reaches 1.74 mW / cm, and the battery has good thermal stability and long-term operation performance; the invention combines big data mining, enzyme engineering and electrochemical technologies, provides a novel enzyme fuel cell solution with high activity, high stability and low cost, and has a good application prospect.

The present application belongs to the technical field of medical equipment, and relates to an electrochemical biosensor for metabolite detection equipment. The sensor has: (1) an electrode system comprising a working electrode and a counter electrode; (2) a reaction reagent arranged on the working electrode and the counter electrode. The reaction reagent comprises: (a) an enzyme; (b) an electron mediator composition; (c) a buffer solution; wherein the electron mediator composition comprises a ruthenium complex and an aniline derivative. The sensor of the present application adopts the same biosensing electrochemical technology route, and as long as the enzyme in the reaction reagent is replaced, electrochemical detection of corresponding metabolites such as blood lactic acid, blood ketone and blood glucose can be realized, which is beneficial to production quality control and improvement of product performance. The sensor of the present application has the advantages of simple manufacturing process, large detection range and rapid and accurate detection.

This invention provides an electrochemical glucose sensor for continuous blood glucose monitoring, belonging to the field of biochemical sensing technology. The sensor includes a substrate and a three-electrode system, wherein the three-electrode system includes a working electrode, a reference electrode, and an auxiliary electrode. The working electrode comprises a conductive substrate, and an electrocatalytic enzyme composite layer and an outer membrane layer sequentially modified on the surface of the conductive substrate, with the outer membrane layer covering the outside of the electrocatalytic enzyme composite layer. The electrocatalytic enzyme composite layer is composed of glucose oxidase, an electron mediator, and a cross-linking agent. The glucose oxidase and the electron mediator are cross-linked and fixed to the surface of the conductive substrate by the cross-linking agent. This invention solves the technical problems of traditional electrochemical glucose sensors, such as long detection time, limited linear response range, low sensitivity and high detection limit, poor stability due to short-term signal drift, weak resistance to interference from sugar impurities and endogenous electroactive small molecules, and poor linearity and large detection error when directly detecting blood samples.

Provided is a new metallocene compound with a formal electron count of 20 to 22. The metallocene compound may be represented by formula (1). This application also provides methods for producing the compound, which can be used, amongst other things, as a catalyst, an electron mediator, or as an electrolyte in a redox flow battery. The unique electronic structure of these compounds provides for novel and highly tunable redox properties.

The invention discloses a PEO-based solid electrolyte with high ionic conductivity and a preparation method of the PEO-based solid electrolyte, and belongs to the technical field of lithium-sulfur batteries. The electrolyte is composed of a PEO matrix, amorphous MOF (aMOF), a free radical polymer and a lithium salt. The aMOF is synthesized through a solvothermal method, the vacancy concentration is regulated and controlled through calcination or reducing agent treatment, lithium ion transmission can be promoted, and polysulfide can be chemically adsorbed; the free radical polymer can be used as an electron mediator for improving the ion transference number and the mechanical strength and synergistically inhibiting the growth of lithium dendrites. The room temperature ionic conductivity of the electrolyte is greater than or equal to 1.2 * 10 <-4 > S cm <-1 >, and the coulombic efficiency is gt; the cycle life of the lithium-sulfur battery is gt; in addition, the stability is kept in a wide temperature range of-20 to 80 DEG C, and the method is suitable for high-energy density (gt; 500 Wh / kg) energy storage system.

The invention discloses a magnetic field modulated electrochemical detection method for horseradish peroxidase, and belongs to the field of electrochemical detection. According to the method, a magnetic field is used for controlling horse radish peroxidase taking magnetic beads as carriers to be in contact with the surface of an electrode of a modified enzyme substrate as required, and ultra-low-amount horse radish peroxidase detection is completed through a time domain detection method and a frequency domain detection method. In time domain detection, an enzyme substrate is fixed on the surface of an electrode, and enzyme is controlled to be far away from and contact with the surface of the electrode through a magnetic field, so that an accurate response signal for shielding noise is obtained. In order to detect a lower amount of enzyme, the magnetic beads are controlled to be in periodic contact with the surface of the electrode by externally adding a periodically changing magnetic field, so that a periodic electrochemical response signal with frequency characteristics is formed. The detection method can be applied to an electrochemical sensing detection system taking horse radish peroxidase or an enzyme-labeled biomarker as a detection object or an electronic mediator, and the detection limit of a traditional horse radish peroxidase electrochemical detection method is improved.

A UiO-66-NH2 / Pt / UiO-66-NH2 catalyst, its preparation, and its application belong to the field of photocatalytic reduction of CO2. It has a "sandwich" structure: defective UiO-66-NH2 serves as the active component substrate, with a noble metal interlayer of Pt acting as the photocatalytic active center, and UiO-66-NH2 as the outer layer. Pt nanoparticles act as electron mediators, thereby efficiently improving photocatalytic activity. The close contact between the highly dispersed Pt and UiO-66-NH2 shortens the electron transport distance and promotes interfacial charge transfer. Furthermore, UiO-66-NH2 not only inhibits Pt aggregation and leaching but also provides more active sites for the reaction, enabling the material to exhibit excellent activity and stability in CO2 reduction under visible light.

The invention discloses an electrochemical immunosensor based on an AuNPs / c-MWCNTs / PTH composite material and a detection platform, firstly, a gold nano solution and a carboxylated multi-walled carbon nanotube particle dispersion liquid are subjected to ultrasonic uniform mixing to prepare the AuNPs / c-MWCNTs composite material, then, an electron mediator thionine is electrically polymerized on the surface of a glassy carbon electrode by using a cyclic voltammetry to obtain a polythionine modified electrode, and finally, the electrochemical immunosensor based on the AuNPs / c-MWCNTs / PTH composite material is obtained. The preparation method comprises the following steps: firstly preparing an AuNPs / c-MWCNTs composite material, then dispensing the AuNPs / c-MWCNTs composite material on a polythionine modified electrode, and finally combining a recognition antigen with the composite material through a covalent bond, the detection range of the sensor on a toxoplasma gondii antibody is 1-1000pg / mL, the detection limit is 0.504 pg / mL, the sensor has good sensitivity, stability and specificity, a new detection technology is provided for diagnosis of toxoplasmosis, and the sensor has good application prospects. The important clinical application value is realized.

The invention relates to the field of organic synthesis and molecular medicine, in particular to a preparation method of a phenazine derivative electron mediator 1-methoxy-5-methylphenazine methyl sulfate salt (1-methoxyl PMS) and application of the phenazine derivative electron mediator 1-methoxy-5-methylphenazine methyl sulfate salt in the electron mediator and a sensor. According to the synthesis method, o-vanillin is taken as a starting raw material, 1-methoxyphenazine is synthesized through m-chloroperoxybenzoic acid oxidation (a solvent-free system), sodium periodate water-phase oxidation and alkaline aqueous solution coupling in sequence, and then methylation reaction is performed in a tetrahydrofuran solvent to prepare a target product. According to the method, a water / THF dual-phase system is innovatively adopted, a'one-pot method 'oxidative coupling simplified process is adopted, acidification pulping replaces traditional column chromatography purification, and a noble metal catalyst is not needed in the whole process. The total yield reaches 60%, the heavy metal residue of the product is less than 1ppm, the solvent toxicity is reduced by 90%, the reaction condition is mild (room temperature / normal pressure), and a new green manufacturing scheme is provided for localization of the CCK-8 kit.

The application relates to the technical field of microbial remediation methods, and specifically discloses an ex-situ microbial remediation method for river sewage, aiming to solve the multiple contradictions of redundancy of existing ex-situ microbial remediation technology, single function of bacterial flora, weak environmental adaptability and difficulty in giving consideration to high efficiency and engineering simplicity. The method comprises the following steps: guiding the river sewage out and pretreating the river sewage to remove large-particle impurities; introducing a biological reactor to inoculate a composite functional bacterial flora composed of the genus Raoultella, the genus Pseudomonas and the genus Bacillus in proportion; implementing gradient dissolved oxygen regulation to realize simultaneous nitrification and denitrification; adding iron-manganese modified biochar as a microbial carrier and an electron mediator; and recycling the biochar after solid-liquid separation and reaction. Through the above technical scheme, the application can realize multi-target and simultaneous high-efficiency removal of ammonia nitrogen, refractory organic matter and trace heavy metals, simplify a process flow, improve system stability and low-temperature adaptability, and support modular deployment and intelligent operation and maintenance.