21 results about "Electron mediator" patented technology

The invention relates to the technical field of biological denitrification, in particular to a nitrogen-doped carbon dot-based microbial electronic control denitrification system and application thereof. The system comprises electron donor microorganisms, electron acceptor microorganisms and nitrogen-doped carbon dots, the nitrogen-doped carbon dots serve as electron mediators outside cells, an efficient extracellular electron transfer network is constructed, and interspecific electron transfer resistance is reduced; meanwhile, the nitrogen-doped carbon dots can regulate and control intracellular electron transport chains and the energy metabolism process, the improvement of the reducing power and the energy level is promoted, and the metabolism mode optimization is realized. By cooperatively regulating and controlling generation, transmission and utilization of electrons under an extreme electron donor limited oligotrophic condition, the system disclosed by the invention can realize efficient denitrification, avoid nitrite accumulation, remarkably inhibit generation of nitrous oxide (N2O), reduce dependence on an external organic carbon source and an electron source, and improve the denitrification efficiency. And a new technical approach is provided for improving the deep denitrification efficiency under the low-carbon condition.

The invention relates to a CdS / denitrifying bacteria hybrid system as well as a construction method and application thereof, and relates to the technical field of water nitrate pollution treatment. Comprising composite denitrifying bacteria, Zn-doped CdS heterojunction nanoparticles loaded on the surface of the composite denitrifying bacteria, and an electronic mediator anchored at the interface of the heterojunction nanoparticles and a flora, the composite denitrifying bacteria are prepared from Stutzeria balearica CXK-1 and Stutzeria balearica GUA-1 according to the volume ratio of the Stutzeria balearica CXK-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 being 1 to (0.8 to 1.2). The hybrid system can utilize photoelectrons to drive microbial denitrification under an illumination condition, so that the reduction of nitrate radicals is realized, and the effect of water treatment is achieved.

The invention belongs to the technical field of coal mine machinery lubrication and hydraulic transmission, and discloses an environment-friendly total-synthesis concentrated solution for a hydraulic support and a preparation method of the concentrated solution. The biological sensing microcapsule corrosion inhibitor is characterized by comprising 0.5%-3.0% of a biological sensing microcapsule, 8.0%-15.0% of a main corrosion inhibitor, 2.0%-6.0% of an auxiliary corrosion inhibitor, 4.0%-10.0% of a water-soluble lubricant, 0.05%-0.2% of an anti-foaming agent, 1.0%-3.0% of a pH buffering agent and deionized water. Wherein the biological sensing microcapsule has a core-shell-shell structure, is integrated with electroactive microorganisms, an electron mediator carrier and a corrosion inhibition precursor release layer, and can trigger targeted release of benzotriazole derivatives when the pH value of a local corrosion microarea is less than or equal to 5.5. The corrosion inhibitor has excellent corrosion protection performance and environment-friendly characteristics, effectively overcomes the problem of inactivation of microorganisms under severe hydraulic working conditions through a microcapsule encapsulation strategy, realizes accurate matching of corrosion signal in-situ sensing and corrosion inhibition response, remarkably improves the anti-rust performance under high-shear and wide-temperature-range working conditions, and has wide application prospects. And meanwhile, the requirements of environmental protection and sealing material compatibility are met.

This invention relates to the field of biological denitrification technology, and more particularly to a microbial electron-regulated denitrification system based on nitrogen-doped carbon dots and its application. The system comprises electron-donating microorganisms, electron-accepting microorganisms, and nitrogen-doped carbon dots. The nitrogen-doped carbon dots act as extracellular electron mediators, constructing an efficient extracellular electron transport network and reducing interspecies electron transport resistance. Simultaneously, the nitrogen-doped carbon dots can regulate intracellular electron transport chains and energy metabolism processes, promoting increased reducing power and energy levels, and optimizing metabolic patterns. By synergistically regulating electron generation, transport, and utilization under oligotrophic conditions with limited electron donors, this system achieves highly efficient denitrification, avoids nitrite accumulation, and significantly inhibits nitrous oxide (N2O) generation, while reducing dependence on external organic carbon sources and electron sources. This provides a new technical approach for improving the efficiency of deep denitrification under low-carbon conditions.

The invention discloses an aqueous zinc ion battery electrolyte for promoting rapid activation and an aqueous zinc ion battery, and relates to the technical field of battery electrolytes. The electrolyte comprises a water solvent, a soluble zinc salt, a soluble manganese salt and persulfate serving as an additive, the molar concentration of the persulfate is 0.001-0.5 mol / L, and the molar concentration of the soluble manganese salt is 0.01-3 mol / L. The persulfate can be selectively adsorbed on the surface of an active site of manganese oxide, and is used as an electron mediator to promote electrochemical regeneration of an ineffective active site in the manganese-based positive electrode material; and meanwhile, manganese ions in the electrolyte can be induced to generate directional deposition on the surface of the positive electrode, so that the repair and supplement of active substances are realized. Through cooperation of the dual effects, the capacity increasing rate of the aqueous zinc ion battery in the initial activation stage is remarkably increased, the battery capacity rapidly reaches the highest stable value, and therefore the comprehensive electrochemical performance of the aqueous zinc ion battery is effectively improved.

The invention discloses heat-resistant glucose dehydrogenase and application thereof in an enzyme fuel cell, and belongs to the crossing field of bioelectrochemistry and new energy technologies. According to the invention, firstly, a heat-resistant glucose dehydrogenase TeGDH is screened, and the heat-resistant glucose dehydrogenase TeGDH is subjected to heterologous expression in escherichia coli BL21 (DE3) and shows good heat stability and catalytic activity; teGDH is dissolved in an anolyte containing an electron mediator anthraquinone-2, 6-disulfonic acid disodium salt, and the heat-resistant high-energy enzyme fuel cell is prepared by adopting a non-immobilized system; the maximum power density of the battery at 50 DEG C reaches 1.74 mW / cm, and the battery has good thermal stability and long-term operation performance; the invention combines big data mining, enzyme engineering and electrochemical technologies, provides a novel enzyme fuel cell solution with high activity, high stability and low cost, and has a good application prospect.

This invention provides an electrochemical glucose sensor for continuous blood glucose monitoring, belonging to the field of biochemical sensing technology. The sensor includes a substrate and a three-electrode system, wherein the three-electrode system includes a working electrode, a reference electrode, and an auxiliary electrode. The working electrode comprises a conductive substrate, and an electrocatalytic enzyme composite layer and an outer membrane layer sequentially modified on the surface of the conductive substrate, with the outer membrane layer covering the outside of the electrocatalytic enzyme composite layer. The electrocatalytic enzyme composite layer is composed of glucose oxidase, an electron mediator, and a cross-linking agent. The glucose oxidase and the electron mediator are cross-linked and fixed to the surface of the conductive substrate by the cross-linking agent. This invention solves the technical problems of traditional electrochemical glucose sensors, such as long detection time, limited linear response range, low sensitivity and high detection limit, poor stability due to short-term signal drift, weak resistance to interference from sugar impurities and endogenous electroactive small molecules, and poor linearity and large detection error when directly detecting blood samples.

Provided is a new metallocene compound with a formal electron count of 20 to 22. The metallocene compound may be represented by formula (1). This application also provides methods for producing the compound, which can be used, amongst other things, as a catalyst, an electron mediator, or as an electrolyte in a redox flow battery. The unique electronic structure of these compounds provides for novel and highly tunable redox properties.

A UiO-66-NH2 / Pt / UiO-66-NH2 catalyst, its preparation, and its application belong to the field of photocatalytic reduction of CO2. It has a "sandwich" structure: defective UiO-66-NH2 serves as the active component substrate, with a noble metal interlayer of Pt acting as the photocatalytic active center, and UiO-66-NH2 as the outer layer. Pt nanoparticles act as electron mediators, thereby efficiently improving photocatalytic activity. The close contact between the highly dispersed Pt and UiO-66-NH2 shortens the electron transport distance and promotes interfacial charge transfer. Furthermore, UiO-66-NH2 not only inhibits Pt aggregation and leaching but also provides more active sites for the reaction, enabling the material to exhibit excellent activity and stability in CO2 reduction under visible light.

The application relates to the technical field of microbial remediation methods, and specifically discloses an ex-situ microbial remediation method for river sewage, aiming to solve the multiple contradictions of redundancy of existing ex-situ microbial remediation technology, single function of bacterial flora, weak environmental adaptability and difficulty in giving consideration to high efficiency and engineering simplicity. The method comprises the following steps: guiding the river sewage out and pretreating the river sewage to remove large-particle impurities; introducing a biological reactor to inoculate a composite functional bacterial flora composed of the genus Raoultella, the genus Pseudomonas and the genus Bacillus in proportion; implementing gradient dissolved oxygen regulation to realize simultaneous nitrification and denitrification; adding iron-manganese modified biochar as a microbial carrier and an electron mediator; and recycling the biochar after solid-liquid separation and reaction. Through the above technical scheme, the application can realize multi-target and simultaneous high-efficiency removal of ammonia nitrogen, refractory organic matter and trace heavy metals, simplify a process flow, improve system stability and low-temperature adaptability, and support modular deployment and intelligent operation and maintenance.

The invention discloses an LDHA enzyme activity detection method based on an MTS color development system. According to the method, a standard substance and a sample to be detected are respectively mixed and reacted with a buffer solution containing excessive pyruvic acid and NADH, then MTS and a phenazine electron mediator are added into a reaction system, and residual NADH is reduced into formazan capable of absorbing visible light at 490 nm, so that weak NADH change is amplified into an end point signal which is easy to detect and high in reproducibility, and the detection sensitivity is high. And substituting the end point signal of the to-be-detected sample into a standard curve constructed by the enzyme activity of the standard substance and the end point signal of the standard substance to obtain the LDHA enzyme activity of the to-be-detected sample. According to the method, the detection sensitivity of LDHA can be improved by about 2-3 times, background interference is remarkably reduced, the method is suitable for high-throughput drug screening and large-scale sample detection, and a sensitive and reliable detection means with industrialization potential is provided for the fields of tumor metabolism research, LDHA targeted drug screening, clinical metabolism marker detection and the like.

The invention discloses a method for removing florfenicol by using an iron-doped biochar reinforced microbial electrolysis cell, which is characterized in that florfenicol in a water body is removed by using the microbial electrolysis cell, and a carbon-based material loaded with iron-doped biochar is used as a cathode in the microbial electrolysis cell. In the invention, the carbon-based material loaded with the iron-doped biochar is used as the cathode, so that the specific surface area and roughness of the electrode can be remarkably increased, florfenicol can be degraded by utilizing different functional microorganisms, and the carbon-based material can be used as an electron mediator to promote extracellular electron transfer between the cathode and the microorganisms, so that the efficient removal of florfenicol is facilitated; the method is used for reducing and degrading florfenicol in a water body, can quickly reduce florfenicol into low-toxicity products, can realize quick removal of florfenicol, has the advantages of simple process, low cost, low energy consumption, high treatment efficiency, good removal effect and the like, is a green technology capable of efficiently treating florfenicol-polluted wastewater, is high in use value and has wide application prospects. The application prospect is good.

This application discloses a redox complex and preparation method therefor and use thereof. The redox complex contains a bridged 2,2′-biimidazole structure, through bridging two nitrogen atoms with active hydrogen, stabilizing the structure of the imidazole ring, preventing conformational isomerism of the 2,2′-biimidazole. When the bridged 2,2′-biimidazole structure coordinates with transition metals to form a complex, which significantly improves the efficiency and stability of the complex. Therefore, when the redox complex is used as the electron mediator in sensors, it exhibits a lower oxidation potential, while significantly extending the service life of the sensors.

This invention belongs to the field of electrochemical sensor technology, specifically relating to an electrochemical sensor for continuous monitoring of lactic acid, its preparation method, and its application. The sensor includes a substrate electrode, a sensitive layer, and a diffusion confinement layer. The sensitive layer, coated on the surface of the substrate electrode, contains a lactic acid oxidation catalyst and an electron mediator. The diffusion confinement layer is a multilayer cross-linked polymer film or a multilayer non-cross-linked polymer film. The electrochemical sensor of this invention precisely controls the pore size and affinity of the membrane layer by adjusting the monomer ratio and graft modification of the diffusion confinement layer copolymer, achieving both high sensitivity and a wide linear detection range. It is highly adaptable and can meet the needs of different concentration ranges in medical monitoring, exercise physiological monitoring, etc. Simultaneously, the curing conditions simulate the human body environment, ensuring that the actual performance is consistent with laboratory tests, and its stability is reliable.

This invention provides a method for hydrogen production from organic wastewater through dark fermentation, belonging to the field of fermentation hydrogen production technology. The method includes: mixing organic wastewater, biochar loaded with metal nanoparticles, and a domesticated butyric acid-producing mixed bacterial community; performing micro-electrolysis and bioactivation treatment to obtain a pretreated solution; then subjecting the pretreated solution to hydrogen-producing phase fermentation treatment, adding an electron mediator to obtain a fermentation broth containing volatile acids; further, subjecting the fermentation broth to de-inhibition phase treatment, using adsorbent materials to adsorb volatile acids, and simultaneously converting some of the adsorbed volatile acids into butyric acid through acid-producing bacteria to obtain a post-fermentation solution; electrolyzing the post-fermentation solution, utilizing electroactive hydrogen-producing bacteria for extracellular electron transfer to convert residual organic matter in the solution into hydrogen gas to obtain crude hydrogen, which is then purified to obtain hydrogen gas. This invention solves the problems of low utilization rate of organic wastewater, inhibition of hydrogen production by volatile acids, and the impact of environmental parameter fluctuations on yield in existing processes.

The invention relates to the technical field of microbial synthesis, and provides a method for promoting microorganisms to reduce carbon dioxide to synthesize multi-carbon fatty acid, which comprises the following steps: in a reactor, inoculating an electroactive carbon chain in a microbial electrosynthesis system to prolong a microbial mixed flora, adding sodium bicarbonate, and aerating by using nitrogen and carbon dioxide to obtain the multi-carbon fatty acid. Applying external voltage to the reactor, and reacting to obtain the multi-carbon fatty acid; the inorganic substrate carbon dioxide is used as a unique carbon source, green electric energy is used as an energy source, conversion of carbon dioxide to fatty acid is promoted, additional organic electron donors or expensive electron mediators do not need to be added, the synthesis process of acetic acid generated by reduction of carbon dioxide to butyric acid and hexanoic acid is remarkably promoted, and the yield of fatty acid is increased. Good application prospects are realized.

This invention discloses a rapid decomposition nutrient release promoter for organic materials and its preparation method, relating to the field of biology. This rapid decomposition nutrient release promoter and its preparation method overcome the limitations of traditional composting agents, achieving a dual improvement in decomposition efficiency and temperature adaptability. Through the synergistic action of psychrophilic functional bacteria, exogenous complex enzymes, and redox electron mediators, decomposition can be initiated within 24 hours at a low temperature of 5℃, and the pile temperature can rise to above 55℃ within 48 hours at temperatures above 10℃. Under normal temperature conditions, the decomposition cycle of organic materials is shortened from 30-45 days to 12-15 days, with a total degradation rate of lignocellulose ≥75%, an improvement of more than 65% compared to conventional microbial agents. The degree of composting fully meets relevant industry standards, effectively solving the industry pain points of difficult fermentation start-up and long cycles in northern autumn and winter seasons, and significantly reducing the cost of organic solid waste treatment.

The invention discloses a disposable electrochemical biosensor as well as a detection method and a reaction substrate composition thereof. The sensor comprises an insulating substrate, an electrode layer and a reagent layer, the electrode layer is configured on the insulating substrate and comprises a working electrode, a background electrode, a reference electrode and a sample suction electrode; the working electrode and the background electrode are coated with the reagent layers, the reagent layers are formed by dispensing or printing reaction reagents on the corresponding electrodes and drying the reaction reagents, and the reaction reagents comprise a buffer reagent, a catalytic reaction substrate, an electron mediator, ascorbic acid oxidase ASO, peroxidase POD, a binder and a stabilizer; wherein the catalytic reaction substrate is AAP or AAG, and is used for respectively carrying out catalytic reaction with ACP or alpha-Glu; the reagent layer coated on the background electrode does not contain a catalytic reaction substrate, and the background electrode is used for deducting an interference signal of endogenous ascorbic acid in a sample. The invention aims to improve the detection accuracy of the sensor.

The application discloses a device and a method for in-situ online analysis of rhizosphere oxygen fluctuation abiotic methane production, which comprises an electrolytic cell composed of a working electrode chamber and a counter electrode chamber separated by a proton exchange membrane. Sterilized nutrient solution is arranged in the counter electrode chamber and the working electrode chamber. The working electrode chamber also contains an electron mediator. A working electrode and a reference electrode are arranged in the working electrode chamber, and a counter electrode is arranged in the counter electrode chamber. The liquid in the working chamber contains the roots of living aquatic plants, which are provided with an iron film and are subjected to sterilization treatment. The working electrode chamber is connected with a gas detection device working electrode chamber in sequence through a gas circulation pipeline, and the working electrode chamber is provided with airtightness. The device and the method are suitable for in-situ quantitative determination of rhizosphere abiotic aerobic methane production of aquatic plants, and can effectively analyze the amount of rhizosphere abiotic methane production in the normal growth process of the plant roots. The device is simple in structure, the method is simple, and the environmental requirements are low.

The application discloses construction and application of multiple electron transfer pathway Escherichia coli, selects non-natural electrically active microorganism Escherichia coli as a research object, and introduces the electron transfer pathway of natural electrically active microorganism into the Escherichia coli. In the Escherichia coli with the Mtr pathway, the appropriate endogenous electron mediator is introduced, and the multiple electron transfer pathways are used to strengthen the transmembrane electron transfer of the Escherichia coli. In order to further improve the electron transfer capacity, the IPTG concentration, the induction temperature, the induction time, the induction initial OD are optimized, the best fermentation condition is selected, the production of phenazine-1-carboxylic acid is improved, and the electron transfer efficiency is improved.

The invention belongs to the technical field of acid wastewater treatment, and particularly relates to a sulfate reducing bacteria nutritional agent as well as a preparation method and application thereof. According to the invention, fermentation liquor of non-edible plants which is rich in amino acids (greater than or equal to 20 g / L) and has soluble chemical oxygen demand greater than or equal to 2200 mg / L is used as a basic carrier, exogenous nutrient substances and electron mediators are compounded, and the fermentation liquor and the nutrient substances of the non-edible plants provide efficient electron donors and nutritional ingredients for sulfate reducing bacteria; the electron mediator improves the electron transfer efficiency of the SRB in an anoxic environment. According to the method, the acidic mine wastewater and the sulfate reducing bacteria are subjected to adaptive culture, so that the SRB adapts to the acidic and high-sulfate environment of AMD, the metabolic efficiency of the sulfate reducing bacteria can be remarkably improved through the synergistic effect of the two modes, the growth rate of the sulfate reducing bacteria and the sulfate reduction rate are increased, and the method has the advantages of low cost, high recycling degree, easiness in large-scale production and the like.