8 results about "Electron mediator" patented technology

This invention provides an electrochemical glucose sensor for continuous blood glucose monitoring, belonging to the field of biochemical sensing technology. The sensor includes a substrate and a three-electrode system, wherein the three-electrode system includes a working electrode, a reference electrode, and an auxiliary electrode. The working electrode comprises a conductive substrate, and an electrocatalytic enzyme composite layer and an outer membrane layer sequentially modified on the surface of the conductive substrate, with the outer membrane layer covering the outside of the electrocatalytic enzyme composite layer. The electrocatalytic enzyme composite layer is composed of glucose oxidase, an electron mediator, and a cross-linking agent. The glucose oxidase and the electron mediator are cross-linked and fixed to the surface of the conductive substrate by the cross-linking agent. This invention solves the technical problems of traditional electrochemical glucose sensors, such as long detection time, limited linear response range, low sensitivity and high detection limit, poor stability due to short-term signal drift, weak resistance to interference from sugar impurities and endogenous electroactive small molecules, and poor linearity and large detection error when directly detecting blood samples.

The application relates to the technical field of microbial remediation methods, and specifically discloses an ex-situ microbial remediation method for river sewage, aiming to solve the multiple contradictions of redundancy of existing ex-situ microbial remediation technology, single function of bacterial flora, weak environmental adaptability and difficulty in giving consideration to high efficiency and engineering simplicity. The method comprises the following steps: guiding the river sewage out and pretreating the river sewage to remove large-particle impurities; introducing a biological reactor to inoculate a composite functional bacterial flora composed of the genus Raoultella, the genus Pseudomonas and the genus Bacillus in proportion; implementing gradient dissolved oxygen regulation to realize simultaneous nitrification and denitrification; adding iron-manganese modified biochar as a microbial carrier and an electron mediator; and recycling the biochar after solid-liquid separation and reaction. Through the above technical scheme, the application can realize multi-target and simultaneous high-efficiency removal of ammonia nitrogen, refractory organic matter and trace heavy metals, simplify a process flow, improve system stability and low-temperature adaptability, and support modular deployment and intelligent operation and maintenance.

This application discloses a redox complex and preparation method therefor and use thereof. The redox complex contains a bridged 2,2′-biimidazole structure, through bridging two nitrogen atoms with active hydrogen, stabilizing the structure of the imidazole ring, preventing conformational isomerism of the 2,2′-biimidazole. When the bridged 2,2′-biimidazole structure coordinates with transition metals to form a complex, which significantly improves the efficiency and stability of the complex. Therefore, when the redox complex is used as the electron mediator in sensors, it exhibits a lower oxidation potential, while significantly extending the service life of the sensors.

This invention belongs to the field of electrochemical sensor technology, specifically relating to an electrochemical sensor for continuous monitoring of lactic acid, its preparation method, and its application. The sensor includes a substrate electrode, a sensitive layer, and a diffusion confinement layer. The sensitive layer, coated on the surface of the substrate electrode, contains a lactic acid oxidation catalyst and an electron mediator. The diffusion confinement layer is a multilayer cross-linked polymer film or a multilayer non-cross-linked polymer film. The electrochemical sensor of this invention precisely controls the pore size and affinity of the membrane layer by adjusting the monomer ratio and graft modification of the diffusion confinement layer copolymer, achieving both high sensitivity and a wide linear detection range. It is highly adaptable and can meet the needs of different concentration ranges in medical monitoring, exercise physiological monitoring, etc. Simultaneously, the curing conditions simulate the human body environment, ensuring that the actual performance is consistent with laboratory tests, and its stability is reliable.

This invention provides a method for hydrogen production from organic wastewater through dark fermentation, belonging to the field of fermentation hydrogen production technology. The method includes: mixing organic wastewater, biochar loaded with metal nanoparticles, and a domesticated butyric acid-producing mixed bacterial community; performing micro-electrolysis and bioactivation treatment to obtain a pretreated solution; then subjecting the pretreated solution to hydrogen-producing phase fermentation treatment, adding an electron mediator to obtain a fermentation broth containing volatile acids; further, subjecting the fermentation broth to de-inhibition phase treatment, using adsorbent materials to adsorb volatile acids, and simultaneously converting some of the adsorbed volatile acids into butyric acid through acid-producing bacteria to obtain a post-fermentation solution; electrolyzing the post-fermentation solution, utilizing electroactive hydrogen-producing bacteria for extracellular electron transfer to convert residual organic matter in the solution into hydrogen gas to obtain crude hydrogen, which is then purified to obtain hydrogen gas. This invention solves the problems of low utilization rate of organic wastewater, inhibition of hydrogen production by volatile acids, and the impact of environmental parameter fluctuations on yield in existing processes.

This invention discloses a rapid decomposition nutrient release promoter for organic materials and its preparation method, relating to the field of biology. This rapid decomposition nutrient release promoter and its preparation method overcome the limitations of traditional composting agents, achieving a dual improvement in decomposition efficiency and temperature adaptability. Through the synergistic action of psychrophilic functional bacteria, exogenous complex enzymes, and redox electron mediators, decomposition can be initiated within 24 hours at a low temperature of 5℃, and the pile temperature can rise to above 55℃ within 48 hours at temperatures above 10℃. Under normal temperature conditions, the decomposition cycle of organic materials is shortened from 30-45 days to 12-15 days, with a total degradation rate of lignocellulose ≥75%, an improvement of more than 65% compared to conventional microbial agents. The degree of composting fully meets relevant industry standards, effectively solving the industry pain points of difficult fermentation start-up and long cycles in northern autumn and winter seasons, and significantly reducing the cost of organic solid waste treatment.

The application discloses a device and a method for in-situ online analysis of rhizosphere oxygen fluctuation abiotic methane production, which comprises an electrolytic cell composed of a working electrode chamber and a counter electrode chamber separated by a proton exchange membrane. Sterilized nutrient solution is arranged in the counter electrode chamber and the working electrode chamber. The working electrode chamber also contains an electron mediator. A working electrode and a reference electrode are arranged in the working electrode chamber, and a counter electrode is arranged in the counter electrode chamber. The liquid in the working chamber contains the roots of living aquatic plants, which are provided with an iron film and are subjected to sterilization treatment. The working electrode chamber is connected with a gas detection device working electrode chamber in sequence through a gas circulation pipeline, and the working electrode chamber is provided with airtightness. The device and the method are suitable for in-situ quantitative determination of rhizosphere abiotic aerobic methane production of aquatic plants, and can effectively analyze the amount of rhizosphere abiotic methane production in the normal growth process of the plant roots. The device is simple in structure, the method is simple, and the environmental requirements are low.

The application discloses construction and application of multiple electron transfer pathway Escherichia coli, selects non-natural electrically active microorganism Escherichia coli as a research object, and introduces the electron transfer pathway of natural electrically active microorganism into the Escherichia coli. In the Escherichia coli with the Mtr pathway, the appropriate endogenous electron mediator is introduced, and the multiple electron transfer pathways are used to strengthen the transmembrane electron transfer of the Escherichia coli. In order to further improve the electron transfer capacity, the IPTG concentration, the induction temperature, the induction time, the induction initial OD are optimized, the best fermentation condition is selected, the production of phenazine-1-carboxylic acid is improved, and the electron transfer efficiency is improved.