Provided in the present invention are a
glucose dehydrogenase mutant and use thereof. The
glucose dehydrogenase mutant comprises: (a) a
protein having an
amino acid sequence shown in SEQ ID NO: 1; or (b) a
protein that has been subjected to
amino acid mutation at at least one of the following sites of the
amino acid sequence in (a): E96, A47, V72, K137, V11, V88, V140, I183, A100, D202, D255, or Q170, and has a
glucose dehydrogenase activity function; and (c) a
protein having at least 80% homology with the amino acid sequence defined in any one of (a) and (b) and having a glucose
dehydrogenase function. The glucose
dehydrogenase mutant of the present application has better tolerance and higher activity under extreme conditions, is suitable for
industrial scale-up, and has low cost and high yield.