414 results about "Adenosine" patented technology

Provided are S-adenosylmethionine synthetase mutants and the use thereof, belonging to the technical field of biological enzyme engineering. Site-directed combinatorial mutations are performed on positions 189, 200, 234, 266 and 371 of S-adenosylmethionine synthetase MAT to obtain a series of mutants with improved enzyme activity, wherein the specific enzyme activity of I189V / V266H and V266H / N371R is increased by more than 10 times relative to the parent. The yields of S-adenosylmethionine synthesized by whole cells of recombinant bacteria expressing mutants I189V / V266H / N371R, V266H / Q234N / N371R and I189V / V266H / Q234N / N371R all reach 1550 mg / L or more. The provided enzyme mutants have broad application prospects in the production of S-adenosylmethionine and the construction of genetically engineered microorganisms for S-adenosylmethionine.

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

The invention discloses an anti-inflammatory and anti-aging composition containing a crocus sativus extract and application of the anti-inflammatory and anti-aging composition, and relates to the technical field of cosmetics, the anti-inflammatory and anti-aging composition comprises the crocus sativus extract, a kangaroo claw extract, a dendrobium devonianum stem extract and adenosine; the anti-inflammatory and anti-aging composition can promote human dermal fibroblasts to secrete III-type collagen, can promote human dermal fibroblasts to proliferate and improve the anti-oxidation and anti-inflammatory capacity of the human dermal fibroblasts, has certain anti-inflammatory and anti-aging effects, and can be widely applied to cosmetics with the anti-inflammatory and anti-aging effects.

The purpose of the present invention is to provide a novel beer-flavored fermented beverage in which the purine concentration is reduced and the deterioration in flavor due to the reduction in purine concentration is improved. The present invention provides a beer-flavored fermented beverage having a purine concentration of 35,000 ppb or less, an adenosine concentration of 15,000 ppb or less, or a guanosine concentration of 56,000 ppb or less, wherein the 5-methylfurfural concentration is 10 ppb or more.

The present disclosure features useful compositions and methods to treat disorders for which deamination of an adenosine in an mRNA produces a therapeutic result.

The present application relates to a kind of pH / H2O2 dual-response drug-loaded hydrogel and its preparation method and application, by ε-polylysine derivative as antitumor active ingredient, with oxidized dextran through Schiff base reaction crosslinking rapid gelation. While using 2-formylphenyl boronic acid covalently package CD73 small molecule inhibitor APCP. ε-polylysine derivative has oncolytic activity, can induce ICD and promote the infiltration of immune cells in tumor cells. CD73 small molecule inhibitor APCP can block extracellular CD39-CD73-adenosine pathway, reduce the level of immunosuppressive metabolite adenosine in tumor tissue, so as to activate the infiltrated immune cells, restore its anti-tumor function. The hydrogel of ε-polylysine derivative and APCP co-delivery synergistically inhibits the effect of tumor growth, and its curative effect is greater than the treatment effect of single drug, and is superior to positive control drug oxaliplatin (OXA). The pH / H2O2 dual-response hydrogel is rapidly gelled, and has good biological safety under mild reaction conditions.

The application discloses a composition for protecting liver and strengthening kidney and reducing uric acid, which is made of the following raw material components: Anoectochilus roxburghii, dried tangerine or orange peel, chicory, gardenia, turmeric, Hovenia dulcis Thunb, Cistanche deserticola Y.C.Ma, Rehmannia glutinosa, potassium citrate, sodium citrate and goose myo-peptide. The composition combines the immortal concept of TCM (Traditional Chinese Medicine) "treating disease from the root" and the uric acid control method of modern nutritional medicine as an organic whole, and achieves multiple effects of protecting liver and strengthening kidney, mutual benefit of yin and yang and reducing uric acid through multiple action targets. Animal experiment results show that the composition can effectively reduce the activities of transaminase ALT and AST, relieve liver damage, reduce serum creatinine level and improve urea ammonia excretion rate, improve kidney function, inhibit the activities of xanthine oxidase and adenosine dehydrogenase and reduce uric acid level. The composition is safe and has no toxic side effects, is suitable for people with insufficient liver and kidney functions and hyperuricemia and has a wide application prospect.

The invention belongs to the field of microbial breeding and fermentation engineering, and particularly relates to a saccharomyces cerevisiae mutagenesis strain with high yield of S-adenosyl-L-methionine as well as a mutagenesis method and application of the saccharomyces cerevisiae mutagenesis strain. Saccharomyces cerevisiae is induced to generate genetic diversity through multiple rounds of ultraviolet mutagenesis, and high-throughput screening is performed in combination with strain color phenotypic difference; and the mutant strain HY2402-Z18 with high yield of S-adenosine-L-methionine and genetic stability is obtained. The SAM yield of the mutagenic strain is increased by 40.1% compared with that of an original strain, the SAM yield reaches 13.44 g / L when the mutagenic strain is fermented for 60 h in a 5 L fermentation tank system, the unit yield reaches 0.156 g / L / OD, and the methionine conversion rate is 75%. After thalli are collected through centrifugation of fermentation liquor, an S-adenosine-L-methionine product with the purity of 98%-100% can be obtained through purification of ion exchange resin, no by-products are accumulated, the yield, the substrate conversion rate and the production efficiency of the S-adenosine-L-methionine can be remarkably improved when the mutagenesis strain is used for fermentation production, and the production cost is reduced. The production cost is effectively reduced, the product purity and quality are improved, and the method is suitable for industrial large-scale production of SAM.

The invention discloses an ergothioneine-producing recombinant engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of gene recombination fermentation. The method comprises the following steps: by taking escherichia coli as a starting bacterium, carrying out recombinant expression on a histidine trimethyl inner salt cysteine sulfoxide synthetase egt1 gene, an L-histidine methyltransferase egtD gene, a histidine trimethyl inner salt cysteine sulfoxide lyase egt2 gene, a methionine adenosine transferase metK gene, an adenylate kinase adK gene and an adenine phosphoribose transferase apt gene; on this basis, supply of precursor substances including histidine, cysteine and methionine is further improved through metabolic transformation, the yield of the finally obtained recombinant engineering bacterium for producing ergothioneine reaches 2.54 g / L after 48 h of shake-flask culture, the yield of a 5L fermentation tank reaches 18 g / L after further enlarged culture, the yield of ergothioneine is guaranteed while energy consumption and cost are reduced, and the method is suitable for industrial production. The method is suitable for practical popularization.

Novel and known substituted purine compounds and salts thereof act as adenosine A2a receptor (A2aR) antagonists for cancer immunotherapy, depression, anxiety, multiple sclerosis, NASH, scleroderma, ADHD, Alzheimer's and Parkinsons. Pharmaceutical compositions comprising such compounds, and methods of their use in treating depression are also taught.

The application discloses a synthesis method of 2'-OMe adenosine, comprising the following steps: S1. dissolving adenosine in DMF, and reacting with a methylation reagent in the presence of a base to obtain a mixture of 2'-OMe adenosine and 3'-OMe adenosine; S2. reacting the mixture of 2'-OMe adenosine and 3'-OMe adenosine with acetic anhydride in a solvent to obtain an acetylated mixture; S3. deacetylating the acetylated mixture under the action of ammonia, and crystallizing to obtain 2'-OMe adenosine; the application uses cheap and easily available raw materials and a safe alkylating reagent, synthesizes 2'-OMe adenosine through a shorter route, avoids column chromatography operation, and provides a new synthesis method of 2'-OMe adenosine which is easy to process.

The present disclosure provides methods of treating a cancer in a subject having an established adenosine fingerprint. An established adenosine fingerprint includes assessing the blood concentration of one or more adenosine machinery proteins, assessing the enzymatic activity of one or more adenosine machinery proteins, and / or assessing the tumor expression level of adenosine machinery proteins. The methods disclosed herein include administering to said subjects a therapeutic agent selected from the group consisting of an agent targeting the extracellular production of adenosine, and an agent antagonizing the activation by adenosine of one of its receptors.

The present invention aims to provide a beverage with increased fullness. In particular, the present invention aims to provide a non-alcoholic beer-taste beverage with increased fullness. The present invention relates to, for example, a non-alcoholic beer-taste beverage containing adenosine at a concentration of 8 ppm or more.

This invention discloses a highly active compound health supplement made from Cordyceps sinensis, Artemisia argyi, and rhubarb, and its preparation method, belonging to the field of health food and pharmaceutical preparation processing technology. The method includes: adding artificial Cordyceps sinensis ultrafine powder, prepared Rhubarb tanguticum fine powder, Artemisia argyi leaf extract, microcrystalline cellulose PH102, and maltodextrin into a mixer and premixing at 25-35 rpm to allow the powder to adhere to the surface of the excipients; after tableting, the tablets are subjected to ultra-high pressure treatment at 350-380 MPa. This invention achieves uniform distribution and performance optimization of the formulation components without damaging the activity of heat-sensitive components through precise coupling of premixing strength and ultra-high pressure modification parameters. Experiments show that the obtained formulation has an adenosine retention rate ≥96%, a dissolution rate ≥85% after 5 minutes, and excellent moisture resistance and mechanical strength, solving the technical problems of large activity loss, easy moisture absorption, and slow dissolution in traditional formulations.

The present invention relates to an RNA hydrogel for tissue regeneration, and more specifically, to an RNA hydrogel for tissue regeneration, which can provide enhanced cell proliferation effect and anti-inflammatory effect, regenerate tissue through wound healing, can be efficiently delivered to cells, does not contain additional chemical components other than nucleic acids, thereby ensuring safety, can deliver a large amount of substances in vivo without toxicity, and can be used for tissue regeneration. The defect that the existing nucleic acid medicine is easy to decompose due to various enzymes in plasma is overcome, and meanwhile, the RNA hydrogel of adenosine is generated in quantity through cell metabolism.

The invention provides a yeast rich in polyphosphate and application of the yeast in synthesis of adenosine triphosphate, and the content of polyphosphate is 15-35 wt% based on the dry matter mass of the yeast rich in polyphosphate; according to the method disclosed by the invention, the yeast is subjected to phosphorus starvation culture and then is subjected to phosphorus supplementary culture, so that the yeast is enriched with polyphosphates, and the yeast rich in polyphosphates is obtained; then, the yeast rich in polyphosphate is used as a biocatalyst to synthesize ATP, that is, self-related enzyme systems, mainly including adenosine kinase, endo-polyphosphate kinase and excision-polyphosphate kinase, in the yeast rich in polyphosphate are used for replacing exogenous polyphosphate kinase and adenosine kinase, and in addition, ATP is synthesized by using the yeast rich in polyphosphate as a biocatalyst. According to the method for synthesizing ATP by using the yeast rich in polyphosphate as the biocatalyst, exogenous adenosine kinase and exogenous polyphosphate kinase do not need to be additionally added, the cost is low, the efficiency of converting adenosine into ATP is high, the batch-to-batch stability of the yeast is realized, and the enzyme activity of the self-related enzyme system is high.

The invention discloses a method for catalytically synthesizing adenosine triphosphate by using microcrystalline cellulose carrier purification immobilized enzyme, which comprises the following steps: preparing bacterial suspension by using escherichia coli wet thalli and Tris-HCL buffer solution, crushing, centrifuging, and taking supernate to obtain crude enzyme; adding a microcrystalline cellulose carrier and the wet thalli into the crude enzyme liquid, and washing the immobilized enzyme after immobilization to obtain the immobilized enzyme; the method comprises the following steps: adding immobilized enzyme adenosine kinase and immobilized polyphosphate kinase into a substrate system with the volume of 1L according to a weight ratio, and starting a catalytic reaction under the conditions that the pH is 5.5-7.5 and the temperature is 20-40 DEG C; after catalysis is finished, the immobilized enzyme adenosine kinase and the immobilized polyphosphate kinase are subjected to solid-liquid separation with reaction liquid through a Buchner funnel, the immobilized enzyme continues to be subjected to catalytic reaction, and the method for preparing the immobilized enzyme is simple in mode, low in cost, easy to industrially produce, high in stability and capable of being repeatedly used for many times, and products are easy to separate.

The application belongs to the technical field of bioengineering, and particularly relates to application of a riboflavin kinase / FMN adenosine transferase truncated protein and a mutant thereof in FMN synthesis. The application obtains a mutant with improved riboflavin kinase enzyme activity by truncating and mutating RibFM-1, and uses the mutant in FMN production. The FMN yield of the strain containing the truncated protein gene is increased by 49.14%, 33.97%, 43.58%, 22.42%, 76.31% and 113.70% respectively compared with the strain containing the non-truncated gene. It can be seen that the riboflavin kinase / FMN adenosine transferase can significantly improve the yield of FMN after being truncated or mutated, and has a wide application potential in FMN industrial production.

The invention provides an antrodia camphorata sporocarp extract, which is prepared from the following raw materials in parts by weight: 20 to 24 parts of wild ganoderma lucidum extract, 8 to 12 parts of herba taraxaci and 8 to 12 parts of bolete. The antrodia camphorata sporocarp extract disclosed by the invention contains various physiologically active components such as triterpenoids, polysaccharides, superoxide disproportionated enzymes, adenosine, proteins (containing immune proteins), vitamins, trace elements, nucleic acid, lectin, amino acid, sterols, lignin, blood pressure stabilizing substances and the like; the traditional Chinese medicine composition can relieve various tumors such as hangover, gastrointestinal discomfort, heatstroke, hypertension, virus infection, diabetes mellitus, nephritis, liver cirrhosis and liver cancer, has the effects of resisting oxidation, resisting inflammation, inhibiting hypertension, protecting liver nerves, inhibiting tumor growth and the like, can bidirectionally regulate immunity and protect the liver, can also treat tumors at multiple targets, and has a wide application prospect. The discomfort of a patient in an anti-tumor process is reduced.

The present invention provides pharmaceutical compositions and methods for use in the treatment of diseases or conditions involving adenosine A2a and / or A2b receptor activity, such as cancer. [Solution] A compound of the following formula I is provided. Also provided are a process for preparing these compounds, pharmaceutical compositions containing them, and their use in the treatment of diseases or conditions involving adenosine A2a and / or A2b receptor activity, such as cancer. JPEG2026086419000278.jpg69170

The present invention relates to a compound, the compound is a compound represented by a formula (I), or an isomer, an isotope derivative, a polymorphic substance, a prodrug, a pharmaceutically acceptable salt or a solvate thereof, and the definitions of A1, B1 and L are the same as the definitions in the specification. Also provided are pharmaceutical compositions and compounds of formula (I) for use in methods of treating related diseases by inhibition of MAT2A, including some cancers in which a gene encoding a methylthioadenosine phosphorylase (MTAP) is deleted and / or not fully functioning.

This invention provides a fluorescence-enhanced composite probe based on gold nanoclusters anchored on a zinc-based coordination polymer, its preparation method, and its application, belonging to the field of probe preparation technology. The method involves first mixing an aqueous solution of glutathione and an aqueous solution of tetrachlorohydrin to obtain a gold nanocluster solution; then adding adenine and water to a 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid buffer solution and stirring until homogeneous, followed by the addition of a zinc chloride solution to obtain a zinc-based coordination polymer; finally, the gold nanocluster solution and the zinc-based coordination polymer solution are mixed homogeneously to obtain the fluorescence-enhanced composite probe based on gold nanoclusters anchored on the zinc-based coordination polymer. The probe of this invention can rapidly and sensitively recognize adenosine triphosphate (ATP). It can also be used to construct fluorescent hydrogels by mixing this material with κ-carrageenan; the hydrogels exhibit a sensitive response to ATP and good self-healing ability.

The present invention is directed to improved materials and methods related to cytosine and adenosine base editing of genomic DNA.

This invention discloses a firming and wrinkle-reducing eye cream, comprising a moisturizer, a whitening agent, a skin repair additive composition, a thickener, a preservative, a film-forming agent, a skin moisturizer, and a surfactant. The weight ratio of the moisturizer, whitening agent, anti-aging additive composition, thickener, preservative, film-forming agent, skin moisturizer, and surfactant is 20:1:2:3:0.3:0.5:1:0.2. The moisturizer includes butylene glycol, glycerin, polydimethylsiloxane, and hydrogenated polyisobutylene. The skin-repairing additive composition includes hydroxypropyl tetrahydropyranotriol, adenosine, cell growth factors, ceramides, and salicylic acid, comprising olefins, betaine, trehalose, 1,2-hexanediol, hydrogenated lecithin, allantoin, and Euglena galactosides. The film-forming agents include polyacrylamide, cyclohexylsiloxane, and polydimethylsiloxane alcohol. The emollients include C13-14 isoparaffins, polydimethylsiloxane / vinyl dimethylsiloxane crosspolymers, and cyclopentamethoxysiloxane. This invention can repair cells and provide long-lasting anti-wrinkle and whitening protection for the corners of the eyes.

The present application belongs to the technical field of contact lenses, and provides a contact lens containing PDRN and a preparation method thereof. The contact lens is pretreated to obtain a plasma lens; the plasma lens is sequentially soaked in a first PDRN solution, a hyaluronic acid solution, a second PDRN solution and a taurine solution to obtain a semi-finished lens; and the semi-finished lens is crosslinked to obtain the contact lens containing PDRN. Through the design of a multi-layer structure, the present application realizes the synchronous and synergistic implantation of PDRN, hyaluronic acid and taurine in the contact lens. Among them, PDRN activates the A2A adenosine receptor signaling pathway, plays a core therapeutic role in promoting corneal epithelial cell proliferation, inhibiting the release of inflammatory factors and accelerating tissue repair; hyaluronic acid plays a role in lubricating and protecting the cornea; and taurine can scavenge active oxygen free radicals and reduce oxidative stress damage.

This invention relates to the regulation of pancreatic islet cell differentiation by regulatory factors related to N6-adenosine methylation modification of mRNA. Specifically, it relates to the use of (a) the ALKBH5 gene, or its protein, or its promoter; and / or (b) the YTHDF2 gene, or its protein, or its inhibitor, for the preparation of compositions or formulations for (1) promoting the differentiation of endodermal cells into pancreatic islet precursor cells and / or islet organoids; and / or (2) preventing and / or treating diabetes. The ALKBH5 of this invention regulates the N6-adenosine methylation modification of mRNA by... 6 Modification A controls the in vitro differentiation process of human pancreatic cells. Furthermore, the method of this invention holds promise as an effective method for in vitro culture of pancreatic islet cells, and could subsequently be applied to the treatment of diabetes.

Cosmetic products containing specific amounts of adenosine and at least one pentose in a cosmetic carrier are suitable for the treatment of hair and / or scalp and lead to - Revitalization of the hair and / or - Stimulation / activation of energy metabolism in hair follicles / hair roots and / or - Stimulation / enhancement of hair growth and / or - Prevention / reduction of premature hair loss and / or - Delaying scalp aging and / or - Increased keratin synthesis and / or - Improvement of the tensile strength of keratin fibers.

The invention relates to the technical field of freeze-drying preparations, in particular to a plasma protective agent, a freeze-drying method for keeping the pH of plasma and freeze-dried plasma, and the plasma protective agent comprises the following components: an instant protective agent which comprises 5mg / ml of glycine; the feed additive is prepared from sodium dihydrogen phosphate, trehalose, hydroxyethyl starch, polyethylene glycol, mannitol, taurine, tryptophan lysine or glutamic acid. The sustained-release protective agent comprises antioxidant components encapsulated by lipidosome, wherein the antioxidant components comprise glutathione, adenosine and vitamin C. The invention also discloses a preparation method of the sustained-release protective agent. The novel protective agent vitamin C is adopted, the plasma pH adjusting operation is remarkably simplified, and the vitamin C is composed of multiple components with different functions, so that the stability of key components such as blood coagulation factors in plasma is greatly improved.

The application provides a method for preparing cross-linking enzyme based on a tyrosinase mutant, the cross-linking enzyme and application thereof. The tyrosinase mutant is mixed with an ultrasonic broken solution of S-adenosylmethionine synthetase containing a tyrosine tag to obtain water-insoluble cross-linking enzyme particles, the cross-linking enzyme is collected by centrifugation or filtration and the like, and enzymatic conversion is carried out. It is found that the cross-linking enzyme of S-adenosylmethionine synthetase can be continuously and stably used for 6 batches, and the activity retention is 85%. The cross-linking enzyme particles prepared by the method have the characteristics of easy recovery, reusability and low cost, and have good industrial application value.